                               SEQUENCE LISTING

<110> AVITIDE, INC.
 
<120> AFFINITY LIGAND LIBRARIES OF THREE-HELIX BUNDLE PROTEINS AND USES
      THEREOF

<130> 1580.00155WO

<140> PCT/US2021/054874
<141> 2021-10-13

<150> 63/188,229
<151> 2021-05-13

<150> 63/091,201
<151> 2020-10-13

<160> 21    

<170> PatentIn version 3.5

<210> 1
<211> 75
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (8)..(9)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (12)..(12)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (16)..(25)
<223> Any amino acid

<220>
<221> SITE
<222> (16)..(25)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (28)..(28)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(32)
<223> Any amino acid

<220>
<221> VARIANT
<222> (34)..(34)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (35)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (39)..(39)
<223> Any amino acid

<220>
<221> VARIANT
<222> (43)..(43)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(75)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220>
<221> SITE
<222> (44)..(75)
<223> /note="This region may encompass one of the following sequences:
      'VD' or 'VDGQAGQGGGSGLNDIFEAQKIEWHEHHHHHH' or 'GQAGQGGGSGLNDIFEA
      QKIEWHEHHHHHH' or 'VDGLNDIFEAQKIEWHEHHHHHH' or 'GLNDIFEAQKIEWHEH
      HHHHH' or it may be absent"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 1
Xaa Gln Arg Arg Xaa Phe Ile Xaa Xaa Leu Arg Xaa Asp Pro Ser Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ala Xaa Leu Leu Ala Xaa 
            20                  25                  30          


Ala Ala Xaa Xaa Asn Asp Xaa Gln Ala Pro Ala Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75  


<210> 2
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 2
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu 
            20                  


<210> 3
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 3
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Lys Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu 
            20                  


<210> 4
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 4
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Val Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu 
            20                  


<210> 5
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 5
Val Asp Ala Lys Phe Glu Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu 
            20                  


<210> 6
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 6
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ile Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu 
            20                  


<210> 7
<211> 24
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 7
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Ala Leu Thr Glu 
            20                  


<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 8
Met Ala Gln Gly Thr 
1               5   


<210> 9
<211> 32
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 9
Val Asp Gly Gln Ala Gly Gln Gly Gly Gly Ser Gly Leu Asn Asp Ile 
1               5                   10                  15      


Phe Glu Ala Gln Lys Ile Glu Trp His Glu His His His His His His 
            20                  25                  30          


<210> 10
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 10
Gly Gln Ala Gly Gln Gly Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu 
1               5                   10                  15      


Ala Gln Lys Ile Glu Trp His Glu His His His His His His 
            20                  25                  30  


<210> 11
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 11
Val Asp Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His 
1               5                   10                  15      


Glu His His His His His His 
            20              


<210> 12
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 12
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu His 
1               5                   10                  15      


His His His His His 
            20      


<210> 13
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 13
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 14
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 14
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Lys Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 15
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 15
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Val Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 16
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 16
Val Asp Ala Lys Phe Glu Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 17
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 17
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ile Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Asn Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 18
<211> 67
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (25)..(25)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (29)..(29)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (32)..(33)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(36)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (40)..(49)
<223> Any amino acid

<220>
<221> SITE
<222> (40)..(49)
<223> /note="This region may encompass 1-10 residues"

<220>
<221> MOD_RES
<222> (52)..(52)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (56)..(56)
<223> Any amino acid

<220>
<221> VARIANT
<222> (58)..(58)
<223> /replace="K" or "R"

<220>
<221> MOD_RES
<222> (59)..(60)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (63)..(63)
<223> Any amino acid

<220>
<221> VARIANT
<222> (67)..(67)
<223> /replace="K" or "R"

<220>
<221> SITE
<222> (1)..(67)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<220> 
<221> source
<223> /note="See specification as filed for detailed description of
      substitutions and preferred embodiments"

<400> 18
Val Asp Ala Lys Phe Asp Lys Glu Leu Glu Glu Ala Arg Ala Glu Ile 
1               5                   10                  15      


Glu Arg Leu Pro Ala Leu Thr Glu Xaa Gln Arg Arg Xaa Phe Ile Xaa 
            20                  25                  30          


Xaa Leu Arg Xaa Asp Pro Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Ser Ala Xaa Leu Leu Ala Xaa Ala Ala Xaa Xaa Asn Asp Xaa Gln 
    50                  55                  60                  


Ala Pro Ala 
65          


<210> 19
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 19
Gly Gly Gly Gly Gly 
1               5   


<210> 20
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 20
Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210> 21
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (8)..(9)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (12)..(12)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (16)..(16)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (19)..(19)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (23)..(23)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (25)..(27)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (30)..(30)
<223> Any amino acid

<400> 21
Xaa Gln Arg Arg Xaa Phe Ile Xaa Xaa Leu Arg Xaa Asp Pro Ser Xaa 
1               5                   10                  15      


Ser Ala Xaa Leu Leu Ala Xaa Ala Xaa Xaa Xaa Asn Asp Xaa 
            20                  25                  30  


