                         SEQUENCE LISTING

<110>  Universiteit Gent
 
<120>  METHODS FOR PROXIMITY MEDIATED COUPLING OF A FIRST AGENT TO A 
       SECOND AGENT

<130>  UG-094-PCT

<150>  20201595.4
<151>  2020-10-13

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-M1


<220>
<221>  modified_base
<222>  (1)..(7)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  acetylation

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys modified on the side chain with 4,7-dioxooctanoyl

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Arg

<400>  1
atgatctnnn                                                              10


<210>  2
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-M2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  modification by acetylation

<220>
<221>  modified_base
<222>  (1)..(7)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys modified on the side chain with 4,7-dioxooctanoyl

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Arg

<400>  2
atcatgtnnn                                                              10


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-M3


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  modification by acetylation

<220>
<221>  modified_base
<222>  (1)..(7)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  Lys modified on the side chain with 4,7-dioxooctanoyl

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Arg

<400>  3
ttatcagnnn                                                              10


<210>  4
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-M4


<220>
<221>  modified_base
<222>  (1)..(11)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  modification by -OOH with O being 2-(2-aminoethoxy)ethoxyacetyl

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  Lys modified on the side chain with 4,7-dioxooctanoyl

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Arg

<400>  4
gtcttgagca gnnn                                                         14


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-A1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H-beta-Ala

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with acetyl

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  5
nnagatcatg cccnnn                                                       16


<210>  6
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-C1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  acetyl-beta-Ala

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with acetyl

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  6
nnagatcatg cccnnn                                                       16


<210>  7
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-H1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H2N-Gly

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with acetyl

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  7
nnagatcatg cccnnn                                                       16


<210>  8
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-Z1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H2N-NHCO(CH2)2CO-Lys modified on the side chain with acetyl

<220>
<221>  modified_base
<222>  (2)..(12)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  Arg

<400>  8
nagatcatgc ccnnn                                                        15


<210>  9
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-S1


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H2N-NHCONH-CH2CO-Lys modified on the side chain with acetyl

<220>
<221>  modified_base
<222>  (2)..(12)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  Arg

<400>  9
nagatcatgc ccnnn                                                        15


<210>  10
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-A2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H-beta-Ala

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with 5-carboxytetramethylrhodamine

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  10
nnagatcatg cccnnn                                                       16


<210>  11
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-H2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  H2N-Gly

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with 5-carboxytetramethylrhodamine

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  11
nnagatcatg cccnnn                                                       16


<210>  12
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PNA-C2


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  acetyl-beta-Ala

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Lys modified on the side chain with 5-carboxytetramethylrhodamine

<220>
<221>  modified_base
<222>  (3)..(13)
<223>  N-(2-aminoethyl) glycine nucleoside

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  Arg

<400>  12
nnagatcatg cccnnn                                                       16


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-DOP-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  ornithine modified on the side chain with 4,7-dioxooctanoyl

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  13

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


<210>  14
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-Hy-coil


<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  ornithine modified on the side chain with H2N-Gly

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  14

Glu Lys Leu Ala Ala Ile Lys Glu Lys Leu Ala Ala Ile Lys Glu Lys 
1               5                   10                  15      


Leu Ala Ala Ile Xaa 
            20      


<210>  15
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-Hy-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  ornithine modified on the side chain with H2N-Gly

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  15

Glu Lys Leu Ala Ala Ile Lys Glu Lys Leu Ala Ala Ile Lys Glu Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Lys 
            20      


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-Hy-coil(R)


<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  ornithine modified on the side chain with H2N-Gly

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  16

Glu Arg Leu Ala Ala Ile Arg Glu Arg Leu Ala Ala Ile Arg Glu Arg 
1               5                   10                  15      


Leu Ala Ala Ile Xaa 
            20      


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-Hy-coil(R)


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  ornithine modified on the side chain with H2N-Gly

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  17

Glu Arg Leu Ala Ala Ile Arg Glu Arg Leu Ala Ala Ile Arg Glu Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Arg 
            20      


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hy-random


<220>
<221>  PEPTIDE
<222>  (8)..(8)
<223>  ornithine modified on the side chain with H2N-Gly

<400>  18

Tyr Lys Ala Asp Tyr Gly Phe Xaa 
1               5               


<210>  19
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-1

<400>  19
gggcatgatc tctgataa                                                     18


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-2

<400>  20
gggcatgatc tccgacaa                                                     18


<210>  21
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-3

<400>  21
gggcatgatc tcaggtta                                                     18


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-4

<400>  22
gggcatgatc tacatgat                                                     18


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-5

<400>  23
gggcatgatc tatataat                                                     18


<210>  24
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-6

<400>  24
gggcatgatc tataagct                                                     18


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-7

<400>  25
gggcatgatc tctgctcaag ac                                                22


<210>  26
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-8

<400>  26
gggcatgatc tccgcgtaat ac                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-9

<400>  27
ggccaggatt tctgctcaag ac                                                22


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-10

<400>  28
ggccaggatt tccgcgtaat ac                                                22


<210>  29
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-11

<400>  29
gggcatgatc ttacacgcta gc                                                22


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-12

<400>  30
aatgtgtgcg cctgctcaag ac                                                22


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA-13

<400>  31
aatgtgtgcg ctacacgcta gc                                                22


<210>  32
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-X1-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  x = ornithine modified on the side chain with 
       (4,7-dioxo-7-phenyl-heptanoyl)

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  32

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


<210>  33
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-X2-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  x = ornithine modified on the side chain with 
       [7-(4-methoxyphenyl)-4,7-dioxo-heptanoyl]

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  33

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


<210>  34
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-X3-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  x = ornithine modified on the side chain with 
       [4,7-dioxo-7-(p-tolyl)heptanoyl]

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  34

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


<210>  35
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-X4-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  x = ornithine modified on the side chain with 
       [7-(4-chlorophenyl)-4,7-dioxo-heptanoyl]

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  35

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


<210>  36
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6-X5-coil


<220>
<221>  PEPTIDE
<222>  (16)..(16)
<223>  x = ornithine modified on the side chain with 
       (4-acetyl-3-methyl-6-oxo-heptanoyl)

<220>
<221>  PEPTIDE
<222>  (21)..(21)
<223>  modification of the C-terminus by 4-acetamidobenzoyl

<400>  36

Lys Glu Leu Ala Ala Ile Glu Lys Glu Leu Ala Ala Ile Glu Lys Xaa 
1               5                   10                  15      


Leu Ala Ala Ile Glu 
            20      


