                         SEQUENCE LISTING

<110>  EveliQure Biotechnologies GmbH
 
<120>  HIGH DOSE SHIGELLA VACCINE PREPARATION

<130>  EQ002P

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm


<220>
<221>  X
<222>  (12)..(12)
<223>  S, N, K or R

<220>
<221>  X
<222>  (13)..(13)
<223>  P, G, L or F

<400>  1

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Xaa Xaa Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  2
<211>  19
<212>  PRT
<213>  E. coli

<400>  2

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  3
<211>  124
<212>  PRT
<213>  E. coli

<400>  3

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Pro Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn 
        115                 120                 


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  4

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Ser Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  5
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  5

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Lys Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  6
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  6

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Arg Pro Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  7
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  7

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Gly Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  8
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  8

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Leu Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  9
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  9

Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Asn Phe Ala Cys Thr 
1               5                   10                  15      


Gly Cys Tyr 
            


<210>  10
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LTB-STm


<220>
<221>  X
<222>  (136)..(136)
<223>  S, N, K or R

<220>
<221>  X
<222>  (137)..(137)
<223>  P, G, L or F

<400>  10

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Pro Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Asn Ser Ser Asn 
        115                 120                 125             


Tyr Cys Cys Glu Leu Cys Cys Xaa Xaa Ala Cys Thr Gly Cys Tyr 
    130                 135                 140             


<210>  11
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LTB-ST(N12S)

<400>  11

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Pro Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Asn Ser Ser Asn 
        115                 120                 125             


Tyr Cys Cys Glu Leu Cys Cys Ser Pro Ala Cys Thr Gly Cys Tyr 
    130                 135                 140             


<210>  12
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LTB-ST(N12S)

<400>  12

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Pro Ser Ser 
1               5                   10                  15      


Leu Cys Ala Tyr Gly Ala Pro Gln Ser Ile Thr Glu Leu Cys Ser Glu 
            20                  25                  30          


Tyr Arg Asn Thr Gln Ile Tyr Thr Ile Asn Asp Lys Ile Leu Ser Tyr 
        35                  40                  45              


Thr Glu Ser Met Ala Gly Lys Arg Glu Met Val Ile Ile Thr Phe Lys 
    50                  55                  60                  


Ser Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Thr 
                85                  90                  95      


Tyr Leu Thr Glu Thr Lys Ile Asp Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro Asn Ser Ile Ala Ala Ile Ser Met Glu Asn Gly Pro Gly Pro 
        115                 120                 125             


Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys Cys Ser Pro Ala Cys Thr 
    130                 135                 140                 


Gly Cys Tyr 
145         


<210>  13
<211>  444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LTB-ST(N12S)

<400>  13
atgaataaag taaaatgtta tgttttattt acggcgttac catcctctct atgtgcatac       60

ggagctcccc agtctattac agaactatgt tcggaatatc gcaacacaca aatatatacg      120

ataaatgaca agatactatc atatacggaa tcgatggcag gcaaaagaga aatggttatc      180

attacattta agagcggcgc aacatttcag gtcgaagtcc cgggcagtca acatatagac      240

tcccaaaaaa aagccattga aaggatgaag gacacattaa gaatcacata tctgaccgag      300

accaaaattg ataaattatg tgtatggaat aataaaaccc ccaattcaat tgcggcaatc      360

agtatggaaa acgggccggg gcccaattct tctaactact gctgtgaact ttgttgttct      420

cctgcctgta caggatgtta ctag                                             444


<210>  14
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LTB-ST(N12S)

<400>  14
atgaataaag taaaatgtta tgttttattt acggcgttac catcctctct atgtgcatac       60

ggagctcccc agtctattac agaactatgt tcggaatatc gcaacacaca aatatatacg      120

ataaatgaca agatactatc atatacggaa tcgatggcag gcaaaagaga aatggttatc      180

attacattta agagcggcgc aacatttcag gtcgaagtcc cgggcagtca acatatagac      240

tcccaaaaaa aagccattga aaggatgaag gacacattaa gaatcacata tctgaccgag      300

accaaaattg ataaattatg tgtatggaat aataaaaccc ccaattcaat tgcggcaatc      360

agtatggaaa acaattcttc taactactgc tgtgaacttt gttgttctcc tgcctgtaca      420

ggatgttact ag                                                          432


<210>  15
<211>  249
<212>  DNA
<213>  E. coli

<400>  15
gctgccgtgg ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca       60

cctttctcac attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta      120

cacattttat cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata      180

ggttaaacaa aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag      240

ttttcctcg                                                              249


<210>  16
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  16
tttgctttaa aagcatgtct aatgctagga acctatataa caactactgt acttatacta       60

atgagcctta tgctgcattt gaactaaagc ggccgccaga tcttccggat ggctcgag        118


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  17
tttgctttaa aagcatgtct aatgaatccg ctcgag                                 36


<210>  18
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Terminator

<400>  18
tttgctttaa aagcatgtct aatgctagga acctatataa caactactgt acttatacta       60

atgagcctta tgctgcattt gaaaaggcgg tagaggatgc aat                        103


<210>  19
<211>  3377
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression construct

<400>  19
gtcgcaaaac atgtcattca ggttcatctc accaataagg atatgagtga agtggaggat       60

aagtgagtct gctgtcagag ttttctggtg tatgtcagta agggtaccac ggtgcttgtt      120

ttcaccacaa gaatgaatgt tttcggcaca tttctcccca gagtgttata attgcggtcg      180

cagagttggt tacgctcatt accccgctgc cgataaggaa tttttcgcgt caggtaacgc      240

ccatcgttta tctcaccgct cccttatacg ttgcgctttt ggtgcggctt agccgtgtgt      300

tttcggagta atgtgccgaa cctgtttgtt gcgatttagc gcgcaaatct ttacttattt      360

acagaacttc ggcattatct tgccggttca aattacggta gtgatacccc agaggattag      420

atggccaaag aagacaatat tgaaatgcaa ggtaccgttc ttgaaacgtt gcctaatacc      480

atgttccgcg tagagttaga aaacggtcac gtggttactg cacacatctc cggtaaaatg      540

cgcaaaaact acatccgcat cctgacgggc gacaaagtga ctgttgaact gaccccgtac      600

gacctgagca aaggccgcat tgtcttccgt agtcgctgat tgttttaccg cctgatgggc      660

gaagagaaag aacgagtaaa aggtcggttt aaccggcctt tttattttgt gatatgtatg      720

aagtactttg gaagtataag tccataactt gtctcgatgt aggcggccgc gctgccgtgg      780

ttcaagtcgc gactaataaa aataatcagg ttgccatgat tcaatgtaca cctttctcac      840

attcgtctcc ggcatgaaaa cgatgcactc tttctttatc gctttcacta cacattttat      900

cctcgcatgg atgttttata aaaaacatga ttgacatcat gttgcatata ggttaaacaa      960

aacaagtggc gttatctttt tccggattgt cttcttgtat gatatataag ttttcctcga     1020

tgaataaagt aaaatgttat gttttattta cggcgttacc atcctctcta tgtgcatacg     1080

gagctcccca gtctattaca gaactatgtt cggaatatcg caacacacaa atatatacga     1140

taaatgacaa gatactatca tatacggaat cgatggcagg caaaagagaa atggttatca     1200

ttacatttaa gagcggcgca acatttcagg tcgaagtccc gggcagtcaa catatagact     1260

cccaaaaaaa agccattgaa aggatgaagg acacattaag aatcacatat ctgaccgaga     1320

ccaaaattga taaattatgt gtatggaata ataaaacccc caattcaatt gcggcaatca     1380

gtatggaaaa cgggccgggg cccaattctt ctaactactg ctgtgaactt tgttgttctc     1440

ctgcctgtac aggatgttac tagtttgctt taaaagcatg tctaatgcta ggaacctata     1500

taacaactac tgtacttata ctaatgagcc ttatgctgca tttgaactaa agcggccgcc     1560

agatcttccg gatggctcga ggctgccgtg gttcaagtcg cgactaataa aaataatcag     1620

gttgccatga ttcaatgtac acctttctca cattcgtctc cggcatgaaa acgatgcact     1680

ctttctttat cgctttcact acacatttta tcctcgcatg gatgttttat aaaaaacatg     1740

attgacatca tgttgcatat aggttaaaca aaacaagtgg cgttatcttt ttccggattg     1800

tcttcttgta tgatatataa gttttcctcg atgaataaag taaaatgtta tgttttattt     1860

acggcgttac catcctctct atgtgcatac ggagctcccc agtctattac agaactatgt     1920

tcggaatatc gcaacacaca aatatatacg ataaatgaca agatactatc atatacggaa     1980

tcgatggcag gcaaaagaga aatggttatc attacattta agagcggcgc aacatttcag     2040

gtcgaagtcc cgggcagtca acatatagac tcccaaaaaa aagccattga aaggatgaag     2100

gacacattaa gaatcacata tctgaccgag accaaaattg ataaattatg tgtatggaat     2160

aataaaaccc ccaattcaat tgcggcaatc agtatggaaa acgggccggg gcccaattct     2220

tctaactact gctgtgaact ttgttgttct cctgcctgta caggatgtta ctagtttgct     2280

ttaaaagcat gtctaatgaa tccgctcgag gctgccgtgg ttcaagtcgc gactaataaa     2340

aataatcagg ttgccatgat tcaatgtaca cctttctcac attcgtctcc ggcatgaaaa     2400

cgatgcactc tttctttatc gctttcacta cacattttat cctcgcatgg atgttttata     2460

aaaaacatga ttgacatcat gttgcatata ggttaaacaa aacaagtggc gttatctttt     2520

tccggattgt cttcttgtat gatatataag ttttcctcga tgaataaagt aaaatgttat     2580

gttttattta cggcgttacc atcctctcta tgtgcatacg gagctcccca gtctattaca     2640

gaactatgtt cggaatatcg caacacacaa atatatacga taaatgacaa gatactatca     2700

tatacggaat cgatggcagg caaaagagaa atggttatca ttacatttaa gagcggcgca     2760

acatttcagg tcgaagtccc gggcagtcaa catatagact cccaaaaaaa agccattgaa     2820

aggatgaagg acacattaag aatcacatat ctgaccgaga ccaaaattga taaattatgt     2880

gtatggaata ataaaacccc caattcaatt gcggcaatca gtatggaaaa cgggccgggg     2940

cccaattctt ctaactactg ctgtgaactt tgttgttctc ctgcctgtac aggatgttac     3000

tagtttgctt taaaagcatg tctaatgcta ggaacctata taacaactac tgtacttata     3060

ctaatgagcc ttatgctgca tttgaaaagg cggtagagga tgcaatgttt aaacgtgtag     3120

gctggagctg cttcgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact     3180

aaggaggata ttcatatgga tgaatgttca ggctatcttt atcttgatgg tggttcagct     3240

gtttggtaaa gaaatcgctg taacaataga agaactgcag gcagtactgg tcccacatcg     3300

tttgctcagt caactggtga agttccgtag cgcgtaaatt aatgctttgc atgaaacgtt     3360

actggagtgg atgaaag                                                    3377


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  20

Gly Pro Gly Pro 
1               


<210>  21
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STm

<400>  21

Glu Asn Gly Pro Gly Pro Asn Ser Ser Asn Tyr Cys Cys Glu Leu Cys 
1               5                   10                  15      


Cys Ser Pro Ala Cys Thr Gly Cys Tyr 
            20                  25  


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
ccccgatata catatgaaaa aatc                                              24


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
tcgcggatcc ttaatagcac ccggtac                                           27


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
aagcaggaga acaacacaat tcac                                              24


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
aagcaggaga acaacacaat tcac                                              24


