                         SEQUENCE LISTING

<110>  Ĵײ̩ҽҩɷ޹˾
       ˼ҩҵ޹˾

<120>  ԽMSLNǶϿԭ弰Ӧ

<130>  IEC200445PCT

<150>  CN202011105495.6
<151>  2020-10-15

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  ˹

<220>
<223>  G16 VH

<400>  1

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Thr Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Arg Ala Ser Gly Tyr Pro Phe Ser Asn Phe 
            20                  25                  30          


Phe Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Asn Pro Ile Gly Gly Ser Thr Thr Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Asp Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Ser His Leu Gly Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser 
        115                 


<210>  2
<211>  108
<212>  PRT
<213>  ˹

<220>
<223>  G16 VL

<400>  2

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Thr Trp 
            20                  25                  30          


Val Ala Trp Tyr Gln His Lys Pro Gly Val Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ser Pro Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala His Ser Phe Pro Ile 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Arg 
            100                 105             


<210>  3
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G16 CDR-H1

<400>  3

Asn Phe Phe Met Asn 
1               5   


<210>  4
<211>  17
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G16 CDR-H2

<400>  4

Ile Ile Asn Pro Ile Gly Gly Ser Thr Thr Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  5
<211>  10
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G16 CDR-H3

<400>  5

Ser Ser Ser His Leu Gly Ala Phe Asp Ile 
1               5                   10  


<210>  6
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G16 CDR-L1

<400>  6

Arg Ala Ser Gln Ser Ile Ser Thr Trp Val Ala 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G16 CDR-L2

<400>  7

Ala Ser Pro Thr Leu Gln Ser 
1               5           


<210>  8
<211>  9
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/IMGT/Chothia G16 CDR-L3

<400>  8

Gln Gln Ala His Ser Phe Pro Ile Thr 
1               5                   


<210>  9
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G16 CDR-H1

<400>  9

Gly Tyr Pro Phe Ser Asn Phe Phe 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G16 CDR-H2

<400>  10

Ile Asn Pro Ile Gly Gly Ser Thr 
1               5               


<210>  11
<211>  12
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G16 CDR-H3

<400>  11

Ala Arg Ser Ser Ser His Leu Gly Ala Phe Asp Ile 
1               5                   10          


<210>  12
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G16 CDR-L1

<400>  12

Gln Ser Ile Ser Thr Trp 
1               5       


<210>  13
<211>  3
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G16 CDR-L2

<400>  13

Ala Ser Pro 
1           


<210>  14
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Chothia  G16 CDR-H1

<400>  14

Gly Tyr Pro Phe Ser Asn Phe 
1               5           


<210>  15
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  Chothia  G16 CDR-H2

<400>  15

Asn Pro Ile Gly Gly Ser 
1               5       


<210>  16
<211>  121
<212>  PRT
<213>  ˹

<220>
<223>  G5 VH

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asp Met Gly Ser Ser Gly Trp Ser Pro Val Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  17
<211>  110
<212>  PRT
<213>  ˹

<220>
<223>  G5 VL

<400>  17

Asp Ile Gln Val Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Asn His Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Leu Gln Thr Gly Val Ser Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Leu Asn Gly Tyr Pro Leu 
                85                  90                  95      


Gly Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110 


<210>  18
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G5 CDR-H1

<400>  18

Asp Tyr Ala Met His 
1               5   


<210>  19
<211>  17
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G5 CDR-H2

<400>  19

Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  20
<211>  12
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G5 CDR-H3

<400>  20

Asp Met Gly Ser Ser Gly Trp Ser Pro Val Asp Tyr 
1               5                   10          


<210>  21
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G5 CDR-L1

<400>  21

Arg Ala Ser Gln Gly Ile Asn His Tyr Leu Ala 
1               5                   10      


<210>  22
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G5 CDR-L2

<400>  22

Gly Ala Ser Thr Leu Gln Thr 
1               5           


<210>  23
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/IMGT/Chothia G5 CDR-L3

<400>  23

Gln Gln Leu Asn Gly Tyr Pro Leu Gly Tyr Thr 
1               5                   10      


<210>  24
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G5 CDR-H1

<400>  24

Gly Phe Thr Phe Asp Asp Tyr Ala 
1               5               


<210>  25
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G5 CDR-H2

<400>  25

Ile Ser Trp Asn Ser Gly Ser Ile 
1               5               


<210>  26
<211>  14
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G5 CDR-H3

<400>  26

Ala Lys Asp Met Gly Ser Ser Gly Trp Ser Pro Val Asp Tyr 
1               5                   10                  


<210>  27
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G5 CDR-L1

<400>  27

Gln Gly Ile Asn His Tyr 
1               5       


<210>  28
<211>  3
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G5 CDR-L2

<400>  28

Gly Ala Ser 
1           


<210>  29
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Chothia G5 CDR-H1

<400>  29

Gly Phe Thr Phe Asp Asp Tyr 
1               5           


<210>  30
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  Chothia G5 CDR-H2

<400>  30

Ser Trp Asn Ser Gly Ser 
1               5       


<210>  31
<211>  121
<212>  PRT
<213>  ˹

<220>
<223>  G9 VH

<400>  31

Glu Val Gln Leu Val Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Tyr Trp Ala Thr Ala Ala Phe Asp Ile Trp Gly 
            100                 105                 110         


Gln Gly Thr Pro Val Thr Val Ser Ser 
        115                 120     


<210>  32
<211>  111
<212>  PRT
<213>  ˹

<220>
<223>  G9 VL

<400>  32

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro 
                85                  90                  95      


Pro Thr Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105                 110     


<210>  33
<211>  5
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G9 CDR-H1

<400>  33

Ser Tyr Ala Met Ser 
1               5   


<210>  34
<211>  17
<212>  PRT
<213>  ˹

<220>
<223>  Kabat G9 CDR-H2

<400>  34

Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  35
<211>  12
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia  G9 CDR-H3

<400>  35

Gly Ser Gly Tyr Trp Ala Thr Ala Ala Phe Asp Ile 
1               5                   10          


<210>  36
<211>  12
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G9 CDR-L1

<400>  36

Arg Ala Ser Gln Ser Val Ser Ser Ser Tyr Leu Ala 
1               5                   10          


<210>  37
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/Chothia G9 CDR-L2

<400>  37

Gly Ala Ser Ser Arg Ala Thr 
1               5           


<210>  38
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  Kabat/IMGT/Chothia G9 CDR-L3

<400>  38

Gln Gln Tyr Gly Ser Ser Pro Pro Thr Trp Thr 
1               5                   10      


<210>  39
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G9 CDR-H1

<400>  39

Gly Phe Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  40
<211>  8
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G9 CDR-H2

<400>  40

Ile Ser Gly Ser Gly Gly Ser Thr 
1               5               


<210>  41
<211>  14
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G9 CDR-H3

<400>  41

Ala Lys Gly Ser Gly Tyr Trp Ala Thr Ala Ala Phe Asp Ile 
1               5                   10                  


<210>  42
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G9 CDR-L1

<400>  42

Gln Ser Val Ser Ser Ser Tyr 
1               5           


<210>  43
<211>  3
<212>  PRT
<213>  ˹

<220>
<223>  IMGT G9 CDR-L2

<400>  43

Gly Ala Ser 
1           


<210>  44
<211>  7
<212>  PRT
<213>  ˹

<220>
<223>  Chothia G9 CDR-H1

<400>  44

Gly Phe Thr Phe Ser Ser Tyr 
1               5           


<210>  45
<211>  6
<212>  PRT
<213>  ˹

<220>
<223>  Chothia G9 CDR-H2

<400>  45

Ser Gly Ser Gly Gly Ser 
1               5       


<210>  46
<211>  357
<212>  DNA
<213>  ˹

<220>
<223>  G16 VH

<400>  46
caggtgcagc tggtgcagag tggtgccgag gtgaccaagc ctggcgcttc cgtgaaggtg       60

tcatgccgtg cttccggcta tcccttcagc aacttcttta tgaactgggt gcgccaggcc      120

ccgggacaag gtctcgagtg gatgggcatc atcaatccaa tcggtggctc cacgacctac      180

gcgcagaagt tccagggacg cgtcactatg acccgcgaca cttccaccga cacggtctac      240

atggagctgt catctctgcg cagcgatgac accgccgtgt actactgtgc tcggtctagc      300

agccacctgg gcgccttcga catatggggc cagggcacta tggtgaccgt gtcctcc         357


<210>  47
<211>  324
<212>  DNA
<213>  ˹

<220>
<223>  G16 VL

<400>  47
gacatccagc tgacccagag tccctcttcc gtgtccgcta gcgtggggga cagggttacc       60

atcacctgcc gagcctccca aagcatctct acctgggtcg cgtggtacca gcacaaaccg      120

ggagtagccc ccaagctgct catttacgca tccccaaccc tgcagagcgg tgtcccgtcc      180

agattttcag ggtcgggctc tgggacagat ttcaccctca ctatttcgtc gcttcagagc      240

gaagactttg ccacctatta ctgtcagcag gcgcattcct tccccatcac cttcggccag      300

ggcactcgcc tggagatcaa gcgc                                             324


<210>  48
<211>  363
<212>  DNA
<213>  ˹

<220>
<223>  G5 VH

<400>  48
caggtgcagc tggtgcaatc tgggggaggc ttggtacagc ctggcaggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttgat gattatgcca tgcactgggt ccggcaagct      120

ccagggaagg gcctggagtg ggtctcaggt attagttgga atagtggtag cataggctat      180

gcggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa ctccctgtat      240

ctgcaaatga acagtctgag agctgaggac acggccttgt attactgtgc aaaagatatg      300

ggtagcagtg gctggtcccc cgttgactac tggggccagg gaaccctggt caccgtctcc      360

tca                                                                    363


<210>  49
<211>  330
<212>  DNA
<213>  ˹

<220>
<223>  G5 VL

<400>  49
gacatccagg tgacccagtc tccatcctcc ctgtctgcat ctgttggaga cagagtcacc       60

atcacttgtc gggccagcca gggcattaac cattatttag cctggtatca acaaaagcca      120

gggaaagccc ctaagctcct gatctacggt gcctccactt tgcaaactgg ggtctcatcg      180

cggttcagcg gcagtggatc tgggacagaa ttcactctca ccatcagcag cctgcagcct      240

gaagattttg caacttatta ctgtcaacag cttaacggtt accctctggg gtacactttt      300

ggccagggga ccaaggtgga aatcaaacgt                                       330


<210>  50
<211>  363
<212>  DNA
<213>  ˹

<220>
<223>  G9 VH

<400>  50
gaggtgcagc tggtggagac cgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggtagt      300

ggttattggg caacggccgc ttttgatatc tggggccagg gaaccccggt caccgtctct      360

tca                                                                    363


<210>  51
<211>  333
<212>  DNA
<213>  ˹

<220>
<223>  G9 VL

<400>  51
gaaattgtgt tgacacagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc       60

ctctcctgca gggccagtca gagtgttagc agcagctact tagcctggta ccagcagaaa      120

cctggccagg ctcccaggct cctcatctat ggtgcatcca gcagggccac tggcatccca      180

gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag      240

cctgaagatt ttgcagtgta ttactgtcag cagtatggta gctcacctcc cacgtggacg      300

ttcggccaag ggaccaaggt ggagatcaaa cgt                                   333


<210>  52
<211>  17
<212>  PRT
<213>  ˹

<220>
<223>  Linker

<400>  52

Ala Ser Gly Gly Gly Gly Ser Gly Gly Arg Ala Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser 
    


<210>  53
<211>  51
<212>  DNA
<213>  ˹

<220>
<223>  Linker

<400>  53
gcttctggcg gcggcgggtc cgggggccgc gcatctggcg gcggtggttc c                51


<210>  54
<211>  244
<212>  PRT
<213>  ˹

<220>
<223>  G16-scFv 

<400>  54

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Thr Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Arg Ala Ser Gly Tyr Pro Phe Ser Asn Phe 
            20                  25                  30          


Phe Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Asn Pro Ile Gly Gly Ser Thr Thr Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Asp Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Ser Ser His Leu Gly Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Arg Ala Ser Gly Gly Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro 
    130                 135                 140                 


Ser Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
145                 150                 155                 160 


Ala Ser Gln Ser Ile Ser Thr Trp Val Ala Trp Tyr Gln His Lys Pro 
                165                 170                 175     


Gly Val Ala Pro Lys Leu Leu Ile Tyr Ala Ser Pro Thr Leu Gln Ser 
            180                 185                 190         


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Thr Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Ala His Ser Phe Pro Ile Thr Phe Gly Gln Gly Thr Arg Leu 
225                 230                 235                 240 


Glu Ile Lys Arg 
                


<210>  55
<211>  732
<212>  DNA
<213>  ˹

<220>
<223>  G16-scFv 

<400>  55
caggtgcagc tggtgcagag tggtgccgag gtgaccaagc ctggcgcttc cgtgaaggtg       60

tcatgccgtg cttccggcta tcccttcagc aacttcttta tgaactgggt gcgccaggcc      120

ccgggacaag gtctcgagtg gatgggcatc atcaatccaa tcggtggctc cacgacctac      180

gcgcagaagt tccagggacg cgtcactatg acccgcgaca cttccaccga cacggtctac      240

atggagctgt catctctgcg cagcgatgac accgccgtgt actactgtgc tcggtctagc      300

agccacctgg gcgccttcga catatggggc cagggcacta tggtgaccgt gtcctccgct      360

tctggcggcg gcgggtccgg gggccgcgca tctggcggcg gtggttccga catccagctg      420

acccagagtc cctcttccgt gtccgctagc gtgggggaca gggttaccat cacctgccga      480

gcctcccaaa gcatctctac ctgggtcgcg tggtaccagc acaaaccggg agtagccccc      540

aagctgctca tttacgcatc cccaaccctg cagagcggtg tcccgtccag attttcaggg      600

tcgggctctg ggacagattt caccctcact atttcgtcgc ttcagagcga agactttgcc      660

acctattact gtcagcaggc gcattccttc cccatcacct tcggccaggg cactcgcctg      720

gagatcaagc gc                                                          732


<210>  56
<211>  248
<212>  PRT
<213>  ˹

<220>
<223>  G5-scFv 

<400>  56

Gln Val Gln Leu Val Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asp Met Gly Ser Ser Gly Trp Ser Pro Val Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Arg Ala Ser Gly Gly Gly Gly Ser Asp Ile Gln Val Thr Gln 
    130                 135                 140                 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Gly Ile Asn His Tyr Leu Ala Trp Tyr Gln Gln 
                165                 170                 175     


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu 
            180                 185                 190         


Gln Thr Gly Val Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Tyr Cys Gln Gln Leu Asn Gly Tyr Pro Leu Gly Tyr Thr Phe Gly Gln 
225                 230                 235                 240 


Gly Thr Lys Val Glu Ile Lys Arg 
                245             


<210>  57
<211>  744
<212>  DNA
<213>  ˹

<220>
<223>  G5-scFv 

<400>  57
caggtgcagc tggtgcaatc tgggggaggc ttggtacagc ctggcaggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttgat gattatgcca tgcactgggt ccggcaagct      120

ccagggaagg gcctggagtg ggtctcaggt attagttgga atagtggtag cataggctat      180

gcggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa ctccctgtat      240

ctgcaaatga acagtctgag agctgaggac acggccttgt attactgtgc aaaagatatg      300

ggtagcagtg gctggtcccc cgttgactac tggggccagg gaaccctggt caccgtctcc      360

tcagctagcg gaggtggcgg ttctggcggg cgcgcctcag gtggaggtgg gtccgacatc      420

caggtgaccc agtctccatc ctccctgtct gcatctgttg gagacagagt caccatcact      480

tgtcgggcca gccagggcat taaccattat ttagcctggt atcaacaaaa gccagggaaa      540

gcccctaagc tcctgatcta cggtgcctcc actttgcaaa ctggggtctc atcgcggttc      600

agcggcagtg gatctgggac agaattcact ctcaccatca gcagcctgca gcctgaagat      660

tttgcaactt attactgtca acagcttaac ggttaccctc tggggtacac ttttggccag      720

gggaccaagg tggaaatcaa acgt                                             744


<210>  58
<211>  249
<212>  PRT
<213>  ˹

<220>
<223>  G9-scFv 

<400>  58

Glu Val Gln Leu Val Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ser Gly Tyr Trp Ala Thr Ala Ala Phe Asp Ile Trp Gly 
            100                 105                 110         


Gln Gly Thr Pro Val Thr Val Ser Ser Ala Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Arg Ala Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln 
    130                 135                 140                 


Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser 
            180                 185                 190         


Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Pro Thr Trp Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys Arg 
                245                 


<210>  59
<211>  747
<212>  DNA
<213>  ˹

<220>
<223>  G9-scFv 

<400>  59
gaggtgcagc tggtggagac cgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac      180

gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaggtagt      300

ggttattggg caacggccgc ttttgatatc tggggccagg gaaccccggt caccgtctct      360

tcagctagcg gaggtggcgg ttctggcggg cgcgcctcag gtggaggtgg gtccgaaatt      420

gtgttgacac agtctccagg caccctgtct ttgtctccag gggaaagagc caccctctcc      480

tgcagggcca gtcagagtgt tagcagcagc tacttagcct ggtaccagca gaaacctggc      540

caggctccca ggctcctcat ctatggtgca tccagcaggg ccactggcat cccagacagg      600

ttcagtggca gtgggtctgg gacagacttc actctcacca tcagcagact ggagcctgaa      660

gattttgcag tgtattactg tcagcagtat ggtagctcac ctcccacgtg gacgttcggc      720

caagggacca aggtggagat caaacgt                                          747


<210>  60
<211>  21
<212>  PRT
<213>  ˹

<220>
<223>  ź1 

<400>  60

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  61
<211>  63
<212>  DNA
<213>  ˹

<220>
<223>  ź1 

<400>  61
atggccttgc ctgttacagc cttgttactg cccctggcgc tgcttctaca cgccgctcgt       60

cct                                                                     63


<210>  62
<211>  45
<212>  PRT
<213>  ˹

<220>
<223>  CD8 

<400>  62

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  63
<211>  135
<212>  DNA
<213>  ˹

<220>
<223>  CD8 

<400>  63
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaagcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  64
<211>  24
<212>  PRT
<213>  ˹

<220>
<223>  CD8Ĥ 

<400>  64

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  65
<211>  72
<212>  DNA
<213>  ˹

<220>
<223>  CD8Ĥ 

<400>  65
atctacattt gggcccctct ggctggtact tgcggggtcc tgctgctttc actcgtgatc       60

actctttact gt                                                           72


<210>  66
<211>  42
<212>  PRT
<213>  ˹

<220>
<223>  źŴṹ(4-1BB) 

<400>  66

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  67
<211>  126
<212>  DNA
<213>  ˹

<220>
<223>  źŴṹ(4-1BB) 

<400>  67
aagcgcggtc ggaagaagct gctgtacatc tttaagcaac ccttcatgag gcctgtgcag       60

actactcaag aggaggacgg ctgttcatgc cggttcccag aggaggagga aggcggctgc      120

gaactg                                                                 126


<210>  68
<211>  112
<212>  PRT
<213>  ˹

<220>
<223>  źŴṹ(CD3) 

<400>  68

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  69
<211>  336
<212>  DNA
<213>  ˹

<220>
<223>  źŴṹ(CD3) 

<400>  69
cgcgtgaaat tcagccgcag cgcagatgct ccagcctaca agcaggggca gaaccagctc       60

tacaacgaac tcaatcttgg tcggagagag gagtacgacg tgctggacaa gcggagagga      120

cgggacccag aaatgggcgg gaagccgcgc agaaagaatc cccaagaggg cctgtacaac      180

gagctccaaa aggataagat ggcagaagcc tatagcgaga ttggtatgaa aggggaacgc      240

agaagaggca aaggccacga cggactgtac cagggactca gcaccgccac caaggacacc      300

tatgacgctc ttcacatgca ggccctgccg cctcgg                                336


<210>  70
<211>  154
<212>  PRT
<213>  ˹

<220>
<223>  źŴṹ(4-1BB-CD3)

<400>  70

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 


<210>  71
<211>  462
<212>  DNA
<213>  ˹

<220>
<223>  źŴṹ(4-1BB-CD3)

<400>  71
aagcgcggtc ggaagaagct gctgtacatc tttaagcaac ccttcatgag gcctgtgcag       60

actactcaag aggaggacgg ctgttcatgc cggttcccag aggaggagga aggcggctgc      120

gaactgcgcg tgaaattcag ccgcagcgca gatgctccag cctacaagca ggggcagaac      180

cagctctaca acgaactcaa tcttggtcgg agagaggagt acgacgtgct ggacaagcgg      240

agaggacggg acccagaaat gggcgggaag ccgcgcagaa agaatcccca agagggcctg      300

tacaacgagc tccaaaagga taagatggca gaagcctata gcgagattgg tatgaaaggg      360

gaacgcagaa gaggcaaagg ccacgacgga ctgtaccagg gactcagcac cgccaccaag      420

gacacctatg acgctcttca catgcaggcc ctgccgcctc gg                         462


<210>  72
<211>  26
<212>  PRT
<213>  ˹

<220>
<223>  P2A 

<400>  72

Arg Ala Lys Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln 
1               5                   10                  15      


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            20                  25      


<210>  73
<211>  78
<212>  DNA
<213>  ˹

<220>
<223>  P2A -1

<400>  73
cgggccaaga gaggatctgg agccacaaat tttagcctgc tgaaacaggc cggagatgtg       60

gaggaaaacc ctggccct                                                     78


<210>  74
<211>  20
<212>  PRT
<213>  ˹

<220>
<223>  ź2 

<400>  74

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  75
<211>  60
<212>  DNA
<213>  ˹

<220>
<223>  ź2 

<400>  75
atgtatagga tgcagctgct gagctgtatt gccctgagcc ttgcactggt gaccaatagc       60


<210>  76
<211>  78
<212>  DNA
<213>  ˹

<220>
<223>  P2A -2

<400>  76
cgagctaaac gaggctcagg cgcgacgaac tttagtttgc tgaagcaagc tggggatgta       60

gaggaaaatc cgggtccc                                                     78


<210>  77
<211>  238
<212>  PRT
<213>  ˹

<220>
<223>  PD1-scFv 

<400>  77

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Asn Trp Val Arg Glu Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asn Pro Lys Tyr Gly Asp Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ile Arg Leu Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala 
    130                 135                 140                 


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Pro Ser Gly Asn Val 
145                 150                 155                 160 


His Asn Tyr Phe Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ser Pro Lys 
                165                 170                 175     


Leu Leu Val Tyr Asn Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser 
        195                 200                 205             


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys His His Tyr Tyr Thr 
    210                 215                 220                 


Ala Pro Phe Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
225                 230                 235             


<210>  78
<211>  714
<212>  DNA
<213>  ˹

<220>
<223>  PD1-scFv 

<400>  78
gaggtgcagc tggtgcagag cggcgccgag gtgaagaagc ctggcgccag cgtgaaggtg       60

agctgcaagg cctccggcta caccttcacc gactactaca tgaactgggt gcgagaggcc      120

cccggccagg gcctggagtg gatgggcgag atcaatccca agtacggcga taccacctac      180

aatcagaagt tcaaggaccg ggccaccctg accgtggata agtctacatc tacagcctat      240

atggagctga gcagcctgag atccgatgat accgcagtgt attattgtgc caaaggaatt      300

agactgtttg actcttgggg ccagggaacc ctagtgaccg taagcagcgg aggaggaggc      360

agcggaggag gcggatctgg cggcggcggc tccgacatcc agctgaccca gagccccagc      420

agcctgagcg ccagcgtggg cgacagggtg accatcacct gccggccctc cggcaacgtg      480

cacaattact tcgcctggta ccagcagaag cccggcaaga gccctaagct gctggtgtat      540

aatgccaaga cactggctga aggcgtgcct agtaggttta gcggcagcgg aagcggcact      600

gattatacac tgacaatttc tagcctgcag cctgaggatt ttgctacata ttattgtcat      660

cattactata cagccccttt tacatttgga ggcggaacaa aggtggagat taag            714


<210>  79
<211>  116
<212>  PRT
<213>  ˹

<220>
<223>  PD1-scFv VH

<400>  79

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Asn Trp Val Arg Glu Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Glu Ile Asn Pro Lys Tyr Gly Asp Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Arg Ala Thr Leu Thr Val Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Gly Ile Arg Leu Phe Asp Ser Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  80
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  PD1-scFv VL

<400>  80

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Pro Ser Gly Asn Val His Asn Tyr 
            20                  25                  30          


Phe Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ser Pro Lys Leu Leu Val 
        35                  40                  45              


Tyr Asn Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys His His Tyr Tyr Thr Ala Pro Phe 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  81
<211>  395
<212>  PRT
<213>  ˹

<220>
<223>  mIL15

<400>  81

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp 
            20                  25                  30          


Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp 
        35                  40                  45              


Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu 
    50                  55                  60                  


Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr 
65                  70                  75                  80  


Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly 
                85                  90                  95      


Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys 
            100                 105                 110         


Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe 
        115                 120                 125             


Ile Asn Thr Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Leu Gln Ile Thr 
145                 150                 155                 160 


Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser 
                165                 170                 175     


Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys 
            180                 185                 190         


Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala 
        195                 200                 205             


Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp 
    210                 215                 220                 


Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr 
225                 230                 235                 240 


Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu 
                245                 250                 255     


Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala 
            260                 265                 270         


Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr 
        275                 280                 285             


Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser 
    290                 295                 300                 


Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln 
305                 310                 315                 320 


Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr Val Ala Ile 
                325                 330                 335     


Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser Leu Leu 
            340                 345                 350         


Ala Cys Tyr Leu Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu 
        355                 360                 365             


Met Glu Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg 
    370                 375                 380                 


Asp Glu Asp Leu Glu Asn Cys Ser His His Leu 
385                 390                 395 


<210>  82
<211>  1185
<212>  DNA
<213>  ˹

<220>
<223>  mIL15

<400>  82
atggattgga cttggatttt gttcctcgtt gccgcagcga ctcgcgtcca tagtaactgg       60

gtgaatgtaa taagtgattt gaaaaaaatt gaagatctta ttcaatctat gcatattgat      120

gctactttat atacggaaag tgatgttcac cccagttgca aagtaacagc aatgaagtgc      180

tttctcttgg agttacaagt tatttcactt gagtccggag atgcaagtat tcatgataca      240

gtagaaaatc tgatcatcct agcaaacaac agtttgtctt ctaatgggaa tgtaacagaa      300

tctggatgca aagaatgtga ggaactggag gaaaaaaata ttaaagaatt tttgcagagt      360

tttgtacata ttgtccaaat gttcatcaac acttcttccg ggggcggcag tggaggtggc      420

ggtagcggcg ggggtggctc tggtggaggc ggctctgggg gcggaagtct gcagatcacg      480

tgccctcccc ccatgtccgt ggaacacgca gacatctggg tcaagagcta cagcttgtac      540

tccagggagc ggtacatttg taactctggt ttcaagcgta aagccggcac gtccagcctg      600

acggagtgcg tgttgaacaa ggccacgaat gtcgcccact ggacaacccc cagtctcaaa      660

tgcattagag accctgccct ggttcaccaa aggccagcgc caccctccac agtaacgacg      720

gcaggggtga ccccacagcc agagagcctc tccccttctg gaaaagagcc cgcagcttca      780

tctcccagct caaacaacac agcggccaca acagcagcta ttgtcccggg ctcccagctg      840

atgccttcaa aatcaccttc cacaggaacc acagagataa gcagtcatga gtcctcccac      900

ggcaccccct ctcagacaac agccaagaac tgggaactca cagcatccgc ctcccaccag      960

ccgccaggtg tgtatccaca gggccacagc gacaccactg tggctatctc cacgtccact     1020

gtcctgctgt gtgggctgag cgctgtgtct ctcctggcat gctacctcaa gtcaaggcaa     1080

actcccccgc tggccagcgt tgaaatggaa gccatggagg ctctgccggt gacttggggg     1140

accagcagca gagatgaaga cttggaaaac tgctctcacc accta                     1185


<210>  83
<211>  488
<212>  PRT
<213>  ˹

<220>
<223>  G16-CAR

<400>  83

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Thr Lys Pro Gly Ala Ser Val Lys Val Ser Cys Arg Ala Ser Gly Tyr 
        35                  40                  45              


Pro Phe Ser Asn Phe Phe Met Asn Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Ile Ile Asn Pro Ile Gly Gly Ser Thr Thr 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser 
                85                  90                  95      


Thr Asp Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Ser Ser Ser His Leu Gly Ala Phe Asp 
        115                 120                 125             


Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Arg Ala Ser Gly Gly Gly Gly Ser Asp Ile Gln 
145                 150                 155                 160 


Leu Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Arg Val 
                165                 170                 175     


Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Thr Trp Val Ala Trp 
            180                 185                 190         


Tyr Gln His Lys Pro Gly Val Ala Pro Lys Leu Leu Ile Tyr Ala Ser 
        195                 200                 205             


Pro Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    210                 215                 220                 


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe 
225                 230                 235                 240 


Ala Thr Tyr Tyr Cys Gln Gln Ala His Ser Phe Pro Ile Thr Phe Gly 
                245                 250                 255     


Gln Gly Thr Arg Leu Glu Ile Lys Arg Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  84
<211>  1464
<212>  DNA
<213>  ˹

<220>
<223>  G16 CAR

<400>  84
atggccttgc ctgttacagc cttgttactg cccctggcgc tgcttctaca cgccgctcgt       60

cctcaggtgc agctggtgca gagtggtgcc gaggtgacca agcctggcgc ttccgtgaag      120

gtgtcatgcc gtgcttccgg ctatcccttc agcaacttct ttatgaactg ggtgcgccag      180

gccccgggac aaggtctcga gtggatgggc atcatcaatc caatcggtgg ctccacgacc      240

tacgcgcaga agttccaggg acgcgtcact atgacccgcg acacttccac cgacacggtc      300

tacatggagc tgtcatctct gcgcagcgat gacaccgccg tgtactactg tgctcggtct      360

agcagccacc tgggcgcctt cgacatatgg ggccagggca ctatggtgac cgtgtcctcc      420

gcttctggcg gcggcgggtc cgggggccgc gcatctggcg gcggtggttc cgacatccag      480

ctgacccaga gtccctcttc cgtgtccgct agcgtggggg acagggttac catcacctgc      540

cgagcctccc aaagcatctc tacctgggtc gcgtggtacc agcacaaacc gggagtagcc      600

cccaagctgc tcatttacgc atccccaacc ctgcagagcg gtgtcccgtc cagattttca      660

gggtcgggct ctgggacaga tttcaccctc actatttcgt cgcttcagag cgaagacttt      720

gccacctatt actgtcagca ggcgcattcc ttccccatca ccttcggcca gggcactcgc      780

ctggagatca agcgcaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagaa gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat atctacattt gggcccctct ggctggtact      960

tgcggggtcc tgctgctttc actcgtgatc actctttact gtaagcgcgg tcggaagaag     1020

ctgctgtaca tctttaagca acccttcatg aggcctgtgc agactactca agaggaggac     1080

ggctgttcat gccggttccc agaggaggag gaaggcggct gcgaactgcg cgtgaaattc     1140

agccgcagcg cagatgctcc agcctacaag caggggcaga accagctcta caacgaactc     1200

aatcttggtc ggagagagga gtacgacgtg ctggacaagc ggagaggacg ggacccagaa     1260

atgggcggga agccgcgcag aaagaatccc caagagggcc tgtacaacga gctccaaaag     1320

gataagatgg cagaagccta tagcgagatt ggtatgaaag gggaacgcag aagaggcaaa     1380

ggccacgacg gactgtacca gggactcagc accgccacca aggacaccta tgacgctctt     1440

cacatgcagg ccctgccgcc tcgg                                            1464


<210>  85
<211>  2316
<212>  DNA
<213>  ˹

<220>
<223>  G16-PD1 CAR

<400>  85
atggccttgc ctgttacagc cttgttactg cccctggcgc tgcttctaca cgccgctcgt       60

cctcaggtgc agctggtgca gagtggtgcc gaggtgacca agcctggcgc ttccgtgaag      120

gtgtcatgcc gtgcttccgg ctatcccttc agcaacttct ttatgaactg ggtgcgccag      180

gccccgggac aaggtctcga gtggatgggc atcatcaatc caatcggtgg ctccacgacc      240

tacgcgcaga agttccaggg acgcgtcact atgacccgcg acacttccac cgacacggtc      300

tacatggagc tgtcatctct gcgcagcgat gacaccgccg tgtactactg tgctcggtct      360

agcagccacc tgggcgcctt cgacatatgg ggccagggca ctatggtgac cgtgtcctcc      420

gcttctggcg gcggcgggtc cgggggccgc gcatctggcg gcggtggttc cgacatccag      480

ctgacccaga gtccctcttc cgtgtccgct agcgtggggg acagggttac catcacctgc      540

cgagcctccc aaagcatctc tacctgggtc gcgtggtacc agcacaaacc gggagtagcc      600

cccaagctgc tcatttacgc atccccaacc ctgcagagcg gtgtcccgtc cagattttca      660

gggtcgggct ctgggacaga tttcaccctc actatttcgt cgcttcagag cgaagacttt      720

gccacctatt actgtcagca ggcgcattcc ttccccatca ccttcggcca gggcactcgc      780

ctggagatca agcgcaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagaa gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat atctacattt gggcccctct ggctggtact      960

tgcggggtcc tgctgctttc actcgtgatc actctttact gtaagcgcgg tcggaagaag     1020

ctgctgtaca tctttaagca acccttcatg aggcctgtgc agactactca agaggaggac     1080

ggctgttcat gccggttccc agaggaggag gaaggcggct gcgaactgcg cgtgaaattc     1140

agccgcagcg cagatgctcc agcctacaag caggggcaga accagctcta caacgaactc     1200

aatcttggtc ggagagagga gtacgacgtg ctggacaagc ggagaggacg ggacccagaa     1260

atgggcggga agccgcgcag aaagaatccc caagagggcc tgtacaacga gctccaaaag     1320

gataagatgg cagaagccta tagcgagatt ggtatgaaag gggaacgcag aagaggcaaa     1380

ggccacgacg gactgtacca gggactcagc accgccacca aggacaccta tgacgctctt     1440

cacatgcagg ccctgccgcc tcggcgggcc aagagaggat ctggagccac aaattttagc     1500

ctgctgaaac aggccggaga tgtggaggaa aaccctggcc ctatgtatag gatgcagctg     1560

ctgagctgta ttgccctgag ccttgcactg gtgaccaata gcgaggtgca gctggtgcag     1620

agcggcgccg aggtgaagaa gcctggcgcc agcgtgaagg tgagctgcaa ggcctccggc     1680

tacaccttca ccgactacta catgaactgg gtgcgagagg cccccggcca gggcctggag     1740

tggatgggcg agatcaatcc caagtacggc gataccacct acaatcagaa gttcaaggac     1800

cgggccaccc tgaccgtgga taagtctaca tctacagcct atatggagct gagcagcctg     1860

agatccgatg ataccgcagt gtattattgt gccaaaggaa ttagactgtt tgactcttgg     1920

ggccagggaa ccctagtgac cgtaagcagc ggaggaggag gcagcggagg aggcggatct     1980

ggcggcggcg gctccgacat ccagctgacc cagagcccca gcagcctgag cgccagcgtg     2040

ggcgacaggg tgaccatcac ctgccggccc tccggcaacg tgcacaatta cttcgcctgg     2100

taccagcaga agcccggcaa gagccctaag ctgctggtgt ataatgccaa gacactggct     2160

gaaggcgtgc ctagtaggtt tagcggcagc ggaagcggca ctgattatac actgacaatt     2220

tctagcctgc agcctgagga ttttgctaca tattattgtc atcattacta tacagcccct     2280

tttacatttg gaggcggaac aaaggtggag attaag                               2316


<210>  86
<211>  2727
<212>  DNA
<213>  ˹

<220>
<223>  G16-mIL15 CAR

<400>  86
atggccttgc ctgttacagc cttgttactg cccctggcgc tgcttctaca cgccgctcgt       60

cctcaggtgc agctggtgca gagtggtgcc gaggtgacca agcctggcgc ttccgtgaag      120

gtgtcatgcc gtgcttccgg ctatcccttc agcaacttct ttatgaactg ggtgcgccag      180

gccccgggac aaggtctcga gtggatgggc atcatcaatc caatcggtgg ctccacgacc      240

tacgcgcaga agttccaggg acgcgtcact atgacccgcg acacttccac cgacacggtc      300

tacatggagc tgtcatctct gcgcagcgat gacaccgccg tgtactactg tgctcggtct      360

agcagccacc tgggcgcctt cgacatatgg ggccagggca ctatggtgac cgtgtcctcc      420

gcttctggcg gcggcgggtc cgggggccgc gcatctggcg gcggtggttc cgacatccag      480

ctgacccaga gtccctcttc cgtgtccgct agcgtggggg acagggttac catcacctgc      540

cgagcctccc aaagcatctc tacctgggtc gcgtggtacc agcacaaacc gggagtagcc      600

cccaagctgc tcatttacgc atccccaacc ctgcagagcg gtgtcccgtc cagattttca      660

gggtcgggct ctgggacaga tttcaccctc actatttcgt cgcttcagag cgaagacttt      720

gccacctatt actgtcagca ggcgcattcc ttccccatca ccttcggcca gggcactcgc      780

ctggagatca agcgcaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagaa gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat atctacattt gggcccctct ggctggtact      960

tgcggggtcc tgctgctttc actcgtgatc actctttact gtaagcgcgg tcggaagaag     1020

ctgctgtaca tctttaagca acccttcatg aggcctgtgc agactactca agaggaggac     1080

ggctgttcat gccggttccc agaggaggag gaaggcggct gcgaactgcg cgtgaaattc     1140

agccgcagcg cagatgctcc agcctacaag caggggcaga accagctcta caacgaactc     1200

aatcttggtc ggagagagga gtacgacgtg ctggacaagc ggagaggacg ggacccagaa     1260

atgggcggga agccgcgcag aaagaatccc caagagggcc tgtacaacga gctccaaaag     1320

gataagatgg cagaagccta tagcgagatt ggtatgaaag gggaacgcag aagaggcaaa     1380

ggccacgacg gactgtacca gggactcagc accgccacca aggacaccta tgacgctctt     1440

cacatgcagg ccctgccgcc tcggcgagct aaacgaggct caggcgcgac gaactttagt     1500

ttgctgaagc aagctgggga tgtagaggaa aatccgggtc ccatggattg gacttggatt     1560

ttgttcctcg ttgccgcagc gactcgcgtc catagtaact gggtgaatgt aataagtgat     1620

ttgaaaaaaa ttgaagatct tattcaatct atgcatattg atgctacttt atatacggaa     1680

agtgatgttc accccagttg caaagtaaca gcaatgaagt gctttctctt ggagttacaa     1740

gttatttcac ttgagtccgg agatgcaagt attcatgata cagtagaaaa tctgatcatc     1800

ctagcaaaca acagtttgtc ttctaatggg aatgtaacag aatctggatg caaagaatgt     1860

gaggaactgg aggaaaaaaa tattaaagaa tttttgcaga gttttgtaca tattgtccaa     1920

atgttcatca acacttcttc cgggggcggc agtggaggtg gcggtagcgg cgggggtggc     1980

tctggtggag gcggctctgg gggcggaagt ctgcagatca cgtgccctcc ccccatgtcc     2040

gtggaacacg cagacatctg ggtcaagagc tacagcttgt actccaggga gcggtacatt     2100

tgtaactctg gtttcaagcg taaagccggc acgtccagcc tgacggagtg cgtgttgaac     2160

aaggccacga atgtcgccca ctggacaacc cccagtctca aatgcattag agaccctgcc     2220

ctggttcacc aaaggccagc gccaccctcc acagtaacga cggcaggggt gaccccacag     2280

ccagagagcc tctccccttc tggaaaagag cccgcagctt catctcccag ctcaaacaac     2340

acagcggcca caacagcagc tattgtcccg ggctcccagc tgatgccttc aaaatcacct     2400

tccacaggaa ccacagagat aagcagtcat gagtcctccc acggcacccc ctctcagaca     2460

acagccaaga actgggaact cacagcatcc gcctcccacc agccgccagg tgtgtatcca     2520

cagggccaca gcgacaccac tgtggctatc tccacgtcca ctgtcctgct gtgtgggctg     2580

agcgctgtgt ctctcctggc atgctacctc aagtcaaggc aaactccccc gctggccagc     2640

gttgaaatgg aagccatgga ggctctgccg gtgacttggg ggaccagcag cagagatgaa     2700

gacttggaaa actgctctca ccaccta                                         2727


<210>  87
<211>  3579
<212>  DNA
<213>  ˹

<220>
<223>  G16-PD1-mIL15 CAR

<400>  87
atggccttgc ctgttacagc cttgttactg cccctggcgc tgcttctaca cgccgctcgt       60

cctcaggtgc agctggtgca gagtggtgcc gaggtgacca agcctggcgc ttccgtgaag      120

gtgtcatgcc gtgcttccgg ctatcccttc agcaacttct ttatgaactg ggtgcgccag      180

gccccgggac aaggtctcga gtggatgggc atcatcaatc caatcggtgg ctccacgacc      240

tacgcgcaga agttccaggg acgcgtcact atgacccgcg acacttccac cgacacggtc      300

tacatggagc tgtcatctct gcgcagcgat gacaccgccg tgtactactg tgctcggtct      360

agcagccacc tgggcgcctt cgacatatgg ggccagggca ctatggtgac cgtgtcctcc      420

gcttctggcg gcggcgggtc cgggggccgc gcatctggcg gcggtggttc cgacatccag      480

ctgacccaga gtccctcttc cgtgtccgct agcgtggggg acagggttac catcacctgc      540

cgagcctccc aaagcatctc tacctgggtc gcgtggtacc agcacaaacc gggagtagcc      600

cccaagctgc tcatttacgc atccccaacc ctgcagagcg gtgtcccgtc cagattttca      660

gggtcgggct ctgggacaga tttcaccctc actatttcgt cgcttcagag cgaagacttt      720

gccacctatt actgtcagca ggcgcattcc ttccccatca ccttcggcca gggcactcgc      780

ctggagatca agcgcaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagaa gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat atctacattt gggcccctct ggctggtact      960

tgcggggtcc tgctgctttc actcgtgatc actctttact gtaagcgcgg tcggaagaag     1020

ctgctgtaca tctttaagca acccttcatg aggcctgtgc agactactca agaggaggac     1080

ggctgttcat gccggttccc agaggaggag gaaggcggct gcgaactgcg cgtgaaattc     1140

agccgcagcg cagatgctcc agcctacaag caggggcaga accagctcta caacgaactc     1200

aatcttggtc ggagagagga gtacgacgtg ctggacaagc ggagaggacg ggacccagaa     1260

atgggcggga agccgcgcag aaagaatccc caagagggcc tgtacaacga gctccaaaag     1320

gataagatgg cagaagccta tagcgagatt ggtatgaaag gggaacgcag aagaggcaaa     1380

ggccacgacg gactgtacca gggactcagc accgccacca aggacaccta tgacgctctt     1440

cacatgcagg ccctgccgcc tcggcgggcc aagagaggat ctggagccac aaattttagc     1500

ctgctgaaac aggccggaga tgtggaggaa aaccctggcc ctatgtatag gatgcagctg     1560

ctgagctgta ttgccctgag ccttgcactg gtgaccaata gcgaggtgca gctggtgcag     1620

agcggcgccg aggtgaagaa gcctggcgcc agcgtgaagg tgagctgcaa ggcctccggc     1680

tacaccttca ccgactacta catgaactgg gtgcgagagg cccccggcca gggcctggag     1740

tggatgggcg agatcaatcc caagtacggc gataccacct acaatcagaa gttcaaggac     1800

cgggccaccc tgaccgtgga taagtctaca tctacagcct atatggagct gagcagcctg     1860

agatccgatg ataccgcagt gtattattgt gccaaaggaa ttagactgtt tgactcttgg     1920

ggccagggaa ccctagtgac cgtaagcagc ggaggaggag gcagcggagg aggcggatct     1980

ggcggcggcg gctccgacat ccagctgacc cagagcccca gcagcctgag cgccagcgtg     2040

ggcgacaggg tgaccatcac ctgccggccc tccggcaacg tgcacaatta cttcgcctgg     2100

taccagcaga agcccggcaa gagccctaag ctgctggtgt ataatgccaa gacactggct     2160

gaaggcgtgc ctagtaggtt tagcggcagc ggaagcggca ctgattatac actgacaatt     2220

tctagcctgc agcctgagga ttttgctaca tattattgtc atcattacta tacagcccct     2280

tttacatttg gaggcggaac aaaggtggag attaagcgag ctaaacgagg ctcaggcgcg     2340

acgaacttta gtttgctgaa gcaagctggg gatgtagagg aaaatccggg tcccatggat     2400

tggacttgga ttttgttcct cgttgccgca gcgactcgcg tccatagtaa ctgggtgaat     2460

gtaataagtg atttgaaaaa aattgaagat cttattcaat ctatgcatat tgatgctact     2520

ttatatacgg aaagtgatgt tcaccccagt tgcaaagtaa cagcaatgaa gtgctttctc     2580

ttggagttac aagttatttc acttgagtcc ggagatgcaa gtattcatga tacagtagaa     2640

aatctgatca tcctagcaaa caacagtttg tcttctaatg ggaatgtaac agaatctgga     2700

tgcaaagaat gtgaggaact ggaggaaaaa aatattaaag aatttttgca gagttttgta     2760

catattgtcc aaatgttcat caacacttct tccgggggcg gcagtggagg tggcggtagc     2820

ggcgggggtg gctctggtgg aggcggctct gggggcggaa gtctgcagat cacgtgccct     2880

ccccccatgt ccgtggaaca cgcagacatc tgggtcaaga gctacagctt gtactccagg     2940

gagcggtaca tttgtaactc tggtttcaag cgtaaagccg gcacgtccag cctgacggag     3000

tgcgtgttga acaaggccac gaatgtcgcc cactggacaa cccccagtct caaatgcatt     3060

agagaccctg ccctggttca ccaaaggcca gcgccaccct ccacagtaac gacggcaggg     3120

gtgaccccac agccagagag cctctcccct tctggaaaag agcccgcagc ttcatctccc     3180

agctcaaaca acacagcggc cacaacagca gctattgtcc cgggctccca gctgatgcct     3240

tcaaaatcac cttccacagg aaccacagag ataagcagtc atgagtcctc ccacggcacc     3300

ccctctcaga caacagccaa gaactgggaa ctcacagcat ccgcctccca ccagccgcca     3360

ggtgtgtatc cacagggcca cagcgacacc actgtggcta tctccacgtc cactgtcctg     3420

ctgtgtgggc tgagcgctgt gtctctcctg gcatgctacc tcaagtcaag gcaaactccc     3480

ccgctggcca gcgttgaaat ggaagccatg gaggctctgc cggtgacttg ggggaccagc     3540

agcagagatg aagacttgga aaactgctct caccaccta                            3579


<210>  88
<211>  492
<212>  PRT
<213>  ˹

<220>
<223>  G5-CAR

<400>  88

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Thr Phe Asp Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Leu Tyr Tyr Cys Ala Lys Asp Met Gly Ser Ser Gly Trp Ser Pro 
        115                 120                 125             


Val Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Arg Ala Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Gln Val Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
                165                 170                 175     


Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Asn His Tyr Leu 
            180                 185                 190         


Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Gly Ala Ser Thr Leu Gln Thr Gly Val Ser Ser Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
225                 230                 235                 240 


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Leu Asn Gly Tyr Pro Leu Gly 
                245                 250                 255     


Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
305                 310                 315                 320 


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
                325                 330                 335     


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            340                 345                 350         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        355                 360                 365             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  89
<211>  1476
<212>  DNA
<213>  ˹

<220>
<223>  G5 CAR

<400>  89
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccgcaggtgc agctggtgca atctggggga ggcttggtac agcctggcag gtccctgaga      120

ctctcctgtg cagcctctgg attcaccttt gatgattatg ccatgcactg ggtccggcaa      180

gctccaggga agggcctgga gtgggtctca ggtattagtt ggaatagtgg tagcataggc      240

tatgcggact ctgtgaaggg ccgattcacc atctccagag acaacgccaa gaactccctg      300

tatctgcaaa tgaacagtct gagagctgag gacacggcct tgtattactg tgcaaaagat      360

atgggtagca gtggctggtc ccccgttgac tactggggcc agggaaccct ggtcaccgtc      420

tcctcagcta gcggaggtgg cggttctggc gggcgcgcct caggtggagg tgggtccgac      480

atccaggtga cccagtctcc atcctccctg tctgcatctg ttggagacag agtcaccatc      540

acttgtcggg ccagccaggg cattaaccat tatttagcct ggtatcaaca aaagccaggg      600

aaagccccta agctcctgat ctacggtgcc tccactttgc aaactggggt ctcatcgcgg      660

ttcagcggca gtggatctgg gacagaattc actctcacca tcagcagcct gcagcctgaa      720

gattttgcaa cttattactg tcaacagctt aacggttacc ctctggggta cacttttggc      780

caggggacca aggtggaaat caaacgtacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aagcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atatctacat ttgggcccct      960

ctggctggta cttgcggggt cctgctgctt tcactcgtga tcactcttta ctgtaagcgc     1020

ggtcggaaga agctgctgta catctttaag caacccttca tgaggcctgt gcagactact     1080

caagaggagg acggctgttc atgccggttc ccagaggagg aggaaggcgg ctgcgaactg     1140

cgcgtgaaat tcagccgcag cgcagatgct ccagcctaca agcaggggca gaaccagctc     1200

tacaacgaac tcaatcttgg tcggagagag gagtacgacg tgctggacaa gcggagagga     1260

cgggacccag aaatgggcgg gaagccgcgc agaaagaatc cccaagaggg cctgtacaac     1320

gagctccaaa aggataagat ggcagaagcc tatagcgaga ttggtatgaa aggggaacgc     1380

agaagaggca aaggccacga cggactgtac cagggactca gcaccgccac caaggacacc     1440

tatgacgctc ttcacatgca ggccctgccg cctcgg                               1476


<210>  90
<211>  493
<212>  PRT
<213>  ˹

<220>
<223>  G9-CAR

<400>  90

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Thr Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Lys Gly Ser Gly Tyr Trp Ala Thr Ala Ala 
        115                 120                 125             


Phe Asp Ile Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Arg Ala Ser Gly Gly Gly Gly Ser Glu 
145                 150                 155                 160 


Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu 
                165                 170                 175     


Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Ser Tyr 
            180                 185                 190         


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        195                 200                 205             


Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro 
225                 230                 235                 240 


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Pro 
                245                 250                 255     


Thr Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  91
<211>  1479
<212>  DNA
<213>  ˹

<220>
<223>  G9 CAR

<400>  91
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggaggtgc agctggtgga gaccggggga ggcttggtac agcctggggg gtccctgaga      120

ctctcctgtg cagcctctgg attcaccttt agcagctatg ccatgagctg ggtccgccag      180

gctccaggga aggggctgga gtgggtctca gctattagtg gtagtggtgg tagcacatac      240

tacgcagact ccgtgaaggg ccggttcacc atctccagag acaattccaa gaacacgctg      300

tatctgcaaa tgaacagcct gagagccgag gacacggccg tatattactg tgcgaaaggt      360

agtggttatt gggcaacggc cgcttttgat atctggggcc agggaacccc ggtcaccgtc      420

tcttcagcta gcggaggtgg cggttctggc gggcgcgcct caggtggagg tgggtccgaa      480

attgtgttga cacagtctcc aggcaccctg tctttgtctc caggggaaag agccaccctc      540

tcctgcaggg ccagtcagag tgttagcagc agctacttag cctggtacca gcagaaacct      600

ggccaggctc ccaggctcct catctatggt gcatccagca gggccactgg catcccagac      660

aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag actggagcct      720

gaagattttg cagtgtatta ctgtcagcag tatggtagct cacctcccac gtggacgttc      780

ggccaaggga ccaaggtgga gatcaaacgt accacgacgc cagcgccgcg accaccaaca      840

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaagcgtg ccggccagcg      900

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catttgggcc      960

cctctggctg gtacttgcgg ggtcctgctg ctttcactcg tgatcactct ttactgtaag     1020

cgcggtcgga agaagctgct gtacatcttt aagcaaccct tcatgaggcc tgtgcagact     1080

actcaagagg aggacggctg ttcatgccgg ttcccagagg aggaggaagg cggctgcgaa     1140

ctgcgcgtga aattcagccg cagcgcagat gctccagcct acaagcaggg gcagaaccag     1200

ctctacaacg aactcaatct tggtcggaga gaggagtacg acgtgctgga caagcggaga     1260

ggacgggacc cagaaatggg cgggaagccg cgcagaaaga atccccaaga gggcctgtac     1320

aacgagctcc aaaaggataa gatggcagaa gcctatagcg agattggtat gaaaggggaa     1380

cgcagaagag gcaaaggcca cgacggactg taccagggac tcagcaccgc caccaaggac     1440

acctatgacg ctcttcacat gcaggccctg ccgcctcgg                            1479


<210>  92
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  PKLT1F

<400>  92
gcttccggct cgtatgttgt                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  PKLT1R

<400>  93
gacaggaggt tgaggcaggt                                                   20


<210>  94
<211>  233
<212>  PRT
<213>  ˹

<220>
<223>  Fchuman IgG1

<400>  94

Pro Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            20                  25                  30          


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        35                  40                  45              


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    50                  55                  60                  


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
65                  70                  75                  80  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                85                  90                  95      


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            100                 105                 110         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        115                 120                 125             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    130                 135                 140                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
145                 150                 155                 160 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                165                 170                 175     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            180                 185                 190         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        195                 200                 205             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    210                 215                 220                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230             


                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               