
                                SEQUENCE LISTING

<110> Jensen, Michael C.
      Johnson, Adam
      Rosser, James

<120> METHODS AND COMPOSITIONS COMPRISING PD1
  CHIMERIC POLYPEPTIDES
  

<130> SCRI.254WO

<150> 63089752            
<151> 2020-10-09  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 573
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1 wild-type domain

<400> 1
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aaggcccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactggtc gtgggagtcg tgggcggact gctgggatct 540
ctggtgctgc tcgtgtgggt gctggccgtg att                              573

<210> 2
<211> 465
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1 (A99L) domain

<400> 2
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg 60
ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc 120
ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg 180
gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc 240
gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg 300
cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc 360
tacctctgtg gggccatctc cctggccccc aagctccaga tcaaagagag cctgcgggca 420
gagctcaggg tgacagagag aagggcagaa gtgcccacag cccac                 465

<210> 3
<211> 240
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD28 intracellular domain

<400> 3
tgtccaagtc ccctatttcc cggaccttct aagccctttt gggtgctggt ggtggttggt 60
ggagtcctgg cttgctatag cttgctagta acagtggcct ttattatttt ctgggtgagg 120
agtaagagga gcaggctcct gcacagtgac tacatgaaca tgactccccg ccgccccggg 180
cccacccgca agcattacca gccctatgcc ccaccacgcg acttcgcagc ctatcgctcc 240

<210> 4
<211> 888
<212> DNA
<213> Artificial Sequence

<220> 
<223> MyD88 domain

<400> 4
atggctgctg gcggacctgg cgctggatct gctgctcctg tgtctagcac cagcagcctg 60
cctctggccg ccctgaatat gagagtgcgg cggagactga gcctgttcct gaacgtgcgg 120
acacaggtgg ccgccgattg gacagctctg gccgaggaaa tggacttcga gtacctggaa 180
atccggcagc tggaaaccca ggccgaccct acaggacgcc tgctggatgc ttggcagggc 240
agaccaggcg cttctgtggg gagactgctg gaactgctga ccaagctggg ccgggacgac 300
gtgctgctgg aactgggccc tagcatcgaa gaggactgcc agaagtacat cctgaagcag 360
cagcaggaag aggccgagaa gcctctgcag gtggcagccg tggatagcag cgtgccaaga 420
acagctgagc tggccggaat caccaccctg gacgatcctc tgggccacat gcccgagaga 480
ttcgacgcct tcatctgcta ctgccccagc gacatccagt tcgtgcagga aatgatcaga 540
cagctggaac agaccaacta ccggctgaag ctgtgcgtgt ccgaccggga tgtgctgcct 600
ggcacctgtg tgtggtctat cgccagcgag ctgatcgaga agcggtgcag acggatggtc 660
gtggtggtgt ccgacgacta cctgcagtcc aaagagtgcg acttccagac caagttcgcc 720
ctgagcctga gccctggcgc ccaccagaag agactgatcc ccatcaagta caaggccatg 780
aagaaagagt tccccagcat cctgcggttc atcaccgtgt gcgactacac caacccctgc 840
accaagtcct ggttctggac cagactggcc aaggccctgt ctctgcct              888

<210> 5
<211> 348
<212> DNA
<213> Artificial Sequence

<220> 
<223> CD2 domain

<400> 5
aagcggaaga agcagcggag cagaagaaac gacgaggaac tggaaaccag agcccacaga 60
gtggccaccg aggaaagagg cagaaagccc caccagatcc ccgcctccac ccctcagaat 120
cctgccacct ctcagcaccc tccacctccc ccaggacaca gaagccaggc ccctagccat 180
agacctccac cacccggaca ccgggtgcag catcagcctc agaaaagacc ccctgcccct 240
agcggcacac aggtgcacca gcagaaaggc cccccactgc ccagacctag agtgcagcct 300
aagcctcctc acggcgctgc cgagaatagc ctgagcccct ccagcaac              348

<210> 6
<211> 705
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(WT):CD28

<400> 6
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg 60
ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc 120
ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg 180
gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc 240
gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg 300
cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc 360
tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca 420
gagctcaggg tgacagagag aagggcagaa gtgcccacag cccactgtcc aagtccccta 480
tttcccggac cttctaagcc cttttgggtg ctggtggtgg ttggtggagt cctggcttgc 540
tatagcttgc tagtaacagt ggcctttatt attttctggg tgaggagtaa gaggagcagg 600
ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac ccgcaagcat 660
taccagccct atgccccacc acgcgacttc gcagcctatc gctcc                 705

<210> 7
<211> 1470
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(WT):MYD88

<400> 7
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aaggcccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactggtc gtgggagtcg tgggcggact gctgggatct 540
ctggtgctgc tcgtgtgggt gctggccgtg attggcggag gaatggctgc tggcggacct 600
ggcgctggat ctgctgctcc tgtgtctagc accagcagcc tgcctctggc cgccctgaat 660
atgagagtgc ggcggagact gagcctgttc ctgaacgtgc ggacacaggt ggccgccgat 720
tggacagctc tggccgagga aatggacttc gagtacctgg aaatccggca gctggaaacc 780
caggccgacc ctacaggacg cctgctggat gcttggcagg gcagaccagg cgcttctgtg 840
gggagactgc tggaactgct gaccaagctg ggccgggacg acgtgctgct ggaactgggc 900
cctagcatcg aagaggactg ccagaagtac atcctgaagc agcagcagga agaggccgag 960
aagcctctgc aggtggcagc cgtggatagc agcgtgccaa gaacagctga gctggccgga 1020
atcaccaccc tggacgatcc tctgggccac atgcccgaga gattcgacgc cttcatctgc 1080
tactgcccca gcgacatcca gttcgtgcag gaaatgatca gacagctgga acagaccaac 1140
taccggctga agctgtgcgt gtccgaccgg gatgtgctgc ctggcacctg tgtgtggtct 1200
atcgccagcg agctgatcga gaagcggtgc agacggatgg tcgtggtggt gtccgacgac 1260
tacctgcagt ccaaagagtg cgacttccag accaagttcg ccctgagcct gagccctggc 1320
gcccaccaga agagactgat ccccatcaag tacaaggcca tgaagaaaga gttccccagc 1380
atcctgcggt tcatcaccgt gtgcgactac accaacccct gcaccaagtc ctggttctgg 1440
accagactgg ccaaggccct gtctctgcct                                  1470

<210> 8
<211> 930
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(WT):CD2

<400> 8
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aaggcccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactggtc gtgggagtcg tgggcggact gctgggatct 540
ctggtgctgc tcgtgtgggt gctggccgtg attggcggag gaaagcggaa gaagcagcgg 600
agcagaagaa acgacgagga actggaaacc agagcccaca gagtggccac cgaggaaaga 660
ggcagaaagc cccaccagat ccccgcctcc acccctcaga atcctgccac ctctcagcac 720
cctccacctc ccccaggaca cagaagccag gcccctagcc atagacctcc accacccgga 780
caccgggtgc agcatcagcc tcagaaaaga ccccctgccc ctagcggcac acaggtgcac 840
cagcagaaag gccccccact gcccagacct agagtgcagc ctaagcctcc tcacggcgct 900
gccgagaata gcctgagccc ctccagcaac                                  930

<210> 9
<211> 705
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(A99L):CD28

<400> 9
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg 60
ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc 120
ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg 180
gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc 240
gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg 300
cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc 360
tacctctgtg gggccatctc cctggccccc aagctccaga tcaaagagag cctgcgggca 420
gagctcaggg tgacagagag aagggcagaa gtgcccacag cccactgtcc aagtccccta 480
tttcccggac cttctaagcc cttttgggtg ctggtggtgg ttggtggagt cctggcttgc 540
tatagcttgc tagtaacagt ggcctttatt attttctggg tgaggagtaa gaggagcagg 600
ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac ccgcaagcat 660
taccagccct atgccccacc acgcgacttc gcagcctatc gctcc                 705

<210> 10
<211> 1470
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(A99L):MYD88

<400> 10
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aagctccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactggtc gtgggagtcg tgggcggact gctgggatct 540
ctggtgctgc tcgtgtgggt gctggccgtg attggcggag gaatggctgc tggcggacct 600
ggcgctggat ctgctgctcc tgtgtctagc accagcagcc tgcctctggc cgccctgaat 660
atgagagtgc ggcggagact gagcctgttc ctgaacgtgc ggacacaggt ggccgccgat 720
tggacagctc tggccgagga aatggacttc gagtacctgg aaatccggca gctggaaacc 780
caggccgacc ctacaggacg cctgctggat gcttggcagg gcagaccagg cgcttctgtg 840
gggagactgc tggaactgct gaccaagctg ggccgggacg acgtgctgct ggaactgggc 900
cctagcatcg aagaggactg ccagaagtac atcctgaagc agcagcagga agaggccgag 960
aagcctctgc aggtggcagc cgtggatagc agcgtgccaa gaacagctga gctggccgga 1020
atcaccaccc tggacgatcc tctgggccac atgcccgaga gattcgacgc cttcatctgc 1080
tactgcccca gcgacatcca gttcgtgcag gaaatgatca gacagctgga acagaccaac 1140
taccggctga agctgtgcgt gtccgaccgg gatgtgctgc ctggcacctg tgtgtggtct 1200
atcgccagcg agctgatcga gaagcggtgc agacggatgg tcgtggtggt gtccgacgac 1260
tacctgcagt ccaaagagtg cgacttccag accaagttcg ccctgagcct gagccctggc 1320
gcccaccaga agagactgat ccccatcaag tacaaggcca tgaagaaaga gttccccagc 1380
atcctgcggt tcatcaccgt gtgcgactac accaacccct gcaccaagtc ctggttctgg 1440
accagactgg ccaaggccct gtctctgcct                                  1470

<210> 11
<211> 930
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1(A99L):CD2

<400> 11
atgcagatcc ctcaggcccc ttggcctgtc gtgtgggctg tgctgcagct gggatggcgg 60
cctggctggt ttctggacag ccccgacaga ccctggaacc cccctacatt ttcccctgcc 120
ctgctggtcg tgaccgaggg cgacaatgcc accttcacct gtagcttcag caacaccagc 180
gagagcttcg tgctgaactg gtacagaatg agccccagca accagaccga caagctggcc 240
gccttccccg aggatagatc tcagcccggc caggactgcc ggttcagagt gacccagctg 300
cccaacggcc gggacttcca catgtctgtc gtgcgggcca gacggaacga cagcggcaca 360
tatctgtgcg gcgccatcag cctggccccc aagctccaga tcaaagagag cctgagagcc 420
gagctgagag tgaccgagag aagggccgaa gtgcctaccg cccaccctag cccatctcca 480
agacctgccg gccagttcca gacactggtc gtgggagtcg tgggcggact gctgggatct 540
ctggtgctgc tcgtgtgggt gctggccgtg attggcggag gaaagcggaa gaagcagcgg 600
agcagaagaa acgacgagga actggaaacc agagcccaca gagtggccac cgaggaaaga 660
ggcagaaagc cccaccagat ccccgcctcc acccctcaga atcctgccac ctctcagcac 720
cctccacctc ccccaggaca cagaagccag gcccctagcc atagacctcc accacccgga 780
caccgggtgc agcatcagcc tcagaaaaga ccccctgccc ctagcggcac acaggtgcac 840
cagcagaaag gccccccact gcccagacct agagtgcagc ctaagcctcc tcacggcgct 900
gccgagaata gcctgagccc ctccagcaac                                  930

<210> 12
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> PD1tm

<400> 12
gtgggagtcg tgggcggact gctgggatct ctggtgctgc tcgtgtgggt gctggccgtg 60
att                                                               63

<210> 13
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> GLY3 linker

<400> 13
ggcggagga                                                         9

<210> 14
<211> 390
<212> DNA
<213> Artificial Sequence

<220> 
<223> Inducible synthetic promoter, iSynPro

<400> 14
ctgcttaggg ttaggcgttt tgcgctgctt cgcgatcgaa tgagtcacat cgatctccgc 60
cccctcttcg agggggcggg gtcgaggagg aaaaactcga atgagtcaca tcgacccttt 120
gatcttcgag gggactttcc ggggtggagc aagcgtgaca agtccacgta tgacccgacc 180
gacgatatcg aagcctacgc gctgaacgcc agccccgatc gaccccgccc cctcgatttc 240
caagaaatcg aatgacatca tctttcgaat gacatcatct ttcgagggga ctttcctcga 300
acttccttcg aggggacttt cctcgagggg actttcctcg aggaggaaaa actcgagtag 360
agtctagact ctacattttg acacccccat                                  390
