                     SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<130> CX2-209WO1
 
<140> 
<141> 2021-10-05

<150> US 63/088,556
<151> 2020-10-07
 
<160> 552

<210> 1
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> WILD TYPE PHOSPHOPENTOMUTASE ENZYME
 
<400> 1
atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180
ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780
gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 2
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> WILD TYPE PHOSPHOPENTOMUTASE ENZYME
 
<400> 2
Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405                                             


<210> 3
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 3
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 4
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 4
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 5
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 5
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggcctggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 6
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 6
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Pro Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 7
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 7
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctgg gctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggacaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 8
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 8
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Gly Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 9
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 9
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg tgcgaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 10
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 10
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ala Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 11
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 11
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 12
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 12
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 13
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 13
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggcctggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 14
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 14
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Pro Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 15
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 15
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 16
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 16
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 17
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 17
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg tgcgaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 18
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 18
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ala Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 19
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 19
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 20
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 20
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 21
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 21
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataagctc tacgaactgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa catggccagg tggttggtgc gggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 22
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 22
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 23
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 23
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggacaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggcctggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 24
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 24
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Pro Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 25
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 25
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 26
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 26
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 27
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 27
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 28
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 28
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 29
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 29
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccaatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgc gggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 30
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 30
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 31
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 31
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccaatcg gcgcgtacgc atgggcgcac gaactgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataagctc tacactctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa catggccagg tggttggtgc gggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaagggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 32
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 32
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Leu Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Thr 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 33
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 33
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgttccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg gagattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat tggcgacaaa 660
gccggtaact tcaaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa ggtggccagg tggtttctgt cggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcga agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 34
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 34
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Glu Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Lys Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Gly Gly Gln Val Val Ser 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Glu Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 35
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 35
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgt ttaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 36
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 36
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Phe Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 37
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 37
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct actctgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 38
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 38
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ser Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 39
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 39
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtt acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 40
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 40
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 41
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 41
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaggagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 42
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 42
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 43
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 43
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctaaggagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 44
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 44
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Lys Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 45
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 45
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggtgccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 46
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 46
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 47
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 47
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc ggggtcgccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 48
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 48
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 49
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 49
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 50
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 50
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 51
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 51
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggcgccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 52
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 52
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 53
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 53
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggtc gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 54
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 54
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Ser Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 55
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 55
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atttgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 56
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 56
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 57
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 57
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct gggtgggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 58
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 58
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Val Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 59
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 59
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaagcgtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 60
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 60
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Ala Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 61
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 61
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgagcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 62
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 62
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Glu Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 63
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 63
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacttgt acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 64
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 64
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Leu Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 65
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 65
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gacggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 66
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 66
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 67
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 67
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gagtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 68
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 68
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Ser Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 69
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 69
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggac gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 70
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 70
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Thr Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 71
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 71
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc atggagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 72
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 72
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Met Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 73
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 73
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gcaggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 74
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 74
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gln Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 75
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 75
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccggggt tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 76
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 76
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Val Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 77
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 77
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca cgaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 78
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 78
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 79
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 79
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgctgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 80
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 80
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 81
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 81
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc ggggcggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 82
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 82
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Arg Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 83
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 83
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgt ctaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 84
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 84
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Ser Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 85
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 85
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 86
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 86
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 87
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 87
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcaggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ttaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
ctgctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 88
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 88
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Val Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Leu Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 89
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 89
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 90
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 90
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 91
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 91
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggggtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 92
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 92
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 93
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 93
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggggtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 94
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 94
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 95
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 95
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggctccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 96
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 96
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 97
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 97
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggctccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 98
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 98
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 99
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 99
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaagatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 100
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 100
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 101
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 101
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggggtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 102
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 102
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 103
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 103
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggctccg 720
accgtgctgt tcaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 104
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 104
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Phe Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 105
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 105
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgccgt cggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 106
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 106
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 107
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 107
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 108
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 108
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 109
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 109
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 110
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 110
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 111
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 111
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcggagcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 112
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 112
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Glu Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 113
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 113
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 114
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 114
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 115
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 115
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 116
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 116
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 117
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 117
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 118
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 118
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 119
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 119
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 120
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 120
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 121
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 121
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 122
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 122
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 123
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 123
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggctccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 124
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 124
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 125
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 125
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc ggggggtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 126
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 126
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 127
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 127
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggggtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 128
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 128
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 129
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 129
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 130
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 130
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 131
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 131
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 132
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 132
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 133
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 133
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 134
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 134
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 135
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 135
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 136
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 136
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 137
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 137
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gagtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 138
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 138
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Ser Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 139
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 139
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg cgaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 140
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 140
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 141
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 141
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaagatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacattt acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 142
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 142
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 143
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 143
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaagatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gagtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcaccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 144
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 144
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Ser Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Thr Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 145
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 145
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaagatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc atggagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gagtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgccgt cggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 146
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 146
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Met Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Ser Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 147
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 147
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 148
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 148
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 149
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 149
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgtgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc atggagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 150
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 150
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Met Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 151
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 151
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaagatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 152
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 152
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 153
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 153
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgt tcaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 154
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 154
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Phe Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 155
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 155
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgtgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc atggagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 156
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 156
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Met Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 157
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 157
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgtgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg cgaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 158
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 158
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 159
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 159
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gagtgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 160
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 160
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Ser Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 161
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 161
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 162
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 162
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 163
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 163
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctatcgagcc gggggctccg 720
accgtgctgc aaaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct actcgaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 164
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 164
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ser Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 165
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 165
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 166
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 166
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 167
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 167
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 168
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 168
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 169
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 169
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atctgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc ggggagtccg 720
accgtgctgt tcaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 170
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 170
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Leu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Ser Pro 
225                 230                 235                 240 
Thr Val Leu Phe Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 171
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 171
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtgtg acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat cagtgttcca gattgcctgt 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 172
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 172
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Val Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 173
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 173
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagtcatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgtgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgccgt cggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 174
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 174
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 175
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 175
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcgtgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gaccgggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg cgaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 176
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 176
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Arg Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Thr Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 177
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 177
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaccgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gcaggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttgccgt cggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 178
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 178
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Pro Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gln Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ala 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 179
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 179
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg acgattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgcc 540
catgaagaaa ctttcggtct ggaaaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctattgagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct actcgaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tagctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 180
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 180
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Glu Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ser Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 181
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 181
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tcttgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 182
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 182
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Leu Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 183
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 183
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtattgggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 184
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 184
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Leu Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 185
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 185
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatctg tgagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 186
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 186
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Cys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 187
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 187
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaaaagatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 188
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 188
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Lys Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 189
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 189
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tcgtgcgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 190
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 190
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Val Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 191
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 191
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgag gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 192
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 192
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Arg Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 193
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 193
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcct tgagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 194
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 194
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Leu Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 195
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 195
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat tgtttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 196
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 196
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Leu Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 197
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 197
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 198
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 198
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 199
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 199
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagatatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 200
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 200
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Asp Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 201
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 201
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 202
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 202
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 203
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 203
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gctgcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 204
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 204
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Ala Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 205
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 205
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aaaaggctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 206
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 206
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Lys Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 207
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 207
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgca gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 208
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 208
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Gln Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 209
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 209
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag attatctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 210
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 210
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Tyr Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 211
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 211
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatactc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 212
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 212
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Thr Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 213
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 213
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctgctgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 214
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 214
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
Ala Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 215
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 215
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatggggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 216
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 216
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Trp Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 217
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 217
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatgtg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 218
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 218
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Val Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 219
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 219
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggttg ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 220
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 220
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Cys Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 221
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 221
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaataa gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 222
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 222
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Lys Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 223
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 223
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cggggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 224
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 224
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Arg Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 225
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 225
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatca gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 226
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 226
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Gln Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 227
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 227
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatcgg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 228
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 228
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 229
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 229
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctggggaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 230
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 230
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
Gly Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 231
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 231
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atatgctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 232
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 232
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Met Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 233
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 233
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtat ggaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 234
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 234
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Met Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 235
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 235
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atgttctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 236
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 236
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Val Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 237
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 237
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgcggcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 238
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 238
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 239
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 239
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcctcgaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 240
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 240
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 241
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 241
atgaaacgtg catttattat gacgatggac tcattcggca tcggctctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 242
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 242
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ser 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 243
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 243
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa acgggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 244
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 244
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Thr Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 245
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 245
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcagtgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 246
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 246
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Ser Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 247
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 247
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc attgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 248
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 248
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Ile Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 249
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 249
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtagttggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 250
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 250
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Val Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 251
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 251
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa tctggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 252
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 252
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Ser Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 253
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 253
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atgatctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 254
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 254
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Asp Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 255
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 255
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcgtggtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 256
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 256
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Val Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 257
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 257
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgctc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 258
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 258
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Ala Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 259
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 259
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaaggtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 260
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 260
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Lys Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 261
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 261
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt tattgatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 262
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 262
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Ile Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 263
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 263
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgg ctaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 264
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 264
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Ala Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 265
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 265
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggattcgctc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 266
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 266
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Ser Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 267
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 267
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaatgtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 268
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 268
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Met Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 269
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 269
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcggctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 270
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 270
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Arg Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 271
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 271
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactgacgg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 272
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 272
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Thr Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 273
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 273
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtat ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 274
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 274
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Ile Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 275
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 275
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcgg ggagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 276
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 276
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Gly Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 277
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 277
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt gtctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 278
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 278
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Cys Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 279
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 279
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 280
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 280
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Glu Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 281
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 281
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatcct 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 282
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 282
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Pro Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 283
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 283
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aattggctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 284
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 284
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Leu Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 285
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 285
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcgaggtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 286
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 286
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Glu Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 287
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 287
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
attaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 288
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 288
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Ile Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 289
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 289
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcaggtggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 290
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 290
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Val Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 291
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 291
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacgtt 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 292
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 292
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Val Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 293
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 293
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatggc tggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 294
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 294
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Ala Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 295
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 295
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggcgaaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 296
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 296
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Ala Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 297
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 297
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgggc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 298
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 298
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Gly Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 299
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 299
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtgt gtctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 300
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 300
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Val Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 301
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 301
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
ggtgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 302
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 302
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Gly Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 303
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 303
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtct ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 304
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 304
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Leu Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 305
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 305
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactgttgg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 306
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 306
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Leu Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 307
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 307
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa gtgggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 308
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 308
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Val Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 309
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 309
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctga agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 310
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 310
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Lys Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 311
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 311
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgaccgt 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 312
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 312
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Arg Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 313
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 313
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atgagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 314
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 314
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Glu Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 315
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 315
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtga gtctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 316
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 316
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Glu Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 317
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 317
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaaccgcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 318
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 318
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Pro Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 319
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 319
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa tatggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 320
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 320
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys Tyr Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 321
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 321
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgcc 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
catggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 322
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 322
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys His Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 323
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 323
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgac gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 324
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 324
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Thr Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 325
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 325
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ctaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 326
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 326
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 327
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 327
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtga ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 328
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 328
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Asp Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 329
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 329
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
cagaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 330
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 330
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Gln Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 331
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 331
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gcccttaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 332
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 332
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Leu Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 333
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 333
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gagggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 334
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 334
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Glu Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 335
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 335
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 336
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 336
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 337
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 337
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggttt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 338
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 338
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Leu Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 339
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 339
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagcgatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 340
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 340
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Ala Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 341
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 341
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gagaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgacggg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 342
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 342
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 343
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 343
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
tggaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcgacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 344
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 344
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Trp Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asp Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 345
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 345
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gagaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgtcggg cctggacgcg 840
ctgtttgacg ccaccatcgt tgagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 346
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 346
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Ser Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Val Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 347
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 347
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
attgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 348
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 348
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 349
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 349
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 350
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 350
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 351
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 351
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 352
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 352
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 353
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 353
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
tggaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgtcggg cctggacgcg 840
ctgtttgacg ccaccatcaa ggagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aacttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 354
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 354
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Trp Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Ser Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 355
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 355
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 356
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 356
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 357
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 357
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattggggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 358
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 358
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Gly Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 359
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 359
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ttccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 360
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 360
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 361
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 361
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggtgccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 362
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 362
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 363
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 363
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggactggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 364
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 364
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 365
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 365
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaggtgtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 366
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 366
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Gly 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 367
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 367
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattg gaaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 368
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 368
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Trp Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 369
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 369
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtagt acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 370
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 370
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Ser Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 371
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 371
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aatttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 372
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 372
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 373
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 373
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aatttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 374
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 374
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 375
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 375
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 376
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 376
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 377
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 377
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac ttcgaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 378
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 378
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Ser Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 379
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 379
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 380
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 380
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 381
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 381
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgtcggg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 382
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 382
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Ser Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 383
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 383
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt gtgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 384
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 384
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Val Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 385
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 385
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcaggtacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 386
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 386
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Arg Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 387
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 387
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
cggtatggca aagccatgtt c                                           1221

 
<210> 388
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 388
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Arg Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 389
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 389
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat tgggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 390
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 390
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Leu Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 391
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 391
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgaccaggg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 392
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 392
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Gln Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 393
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 393
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt cgttttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 394
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 394
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Arg Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 395
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 395
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
tggaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 396
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 396
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Trp Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 397
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 397
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtac ggccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 398
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 398
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Arg Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 399
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 399
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgcgaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 400
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 400
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Ala Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 401
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 401
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgaggg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 402
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 402
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Glu Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 403
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 403
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgcgtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 404
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 404
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Arg Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 405
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 405
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcgt agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 406
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 406
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Val Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 407
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 407
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgccgggcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 408
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 408
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 409
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 409
atgaaacgtg catttattat gacgatggac tcattcggca tcggcgctac agaagatgca 60
gaaaagtttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagc agggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tggtaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acagcaatcg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tctgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 410
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 410
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Gln Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ser Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Leu Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 411
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 411
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 412
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 412
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 413
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 413
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 414
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 414
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 415
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 415
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 416
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 416
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 417
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 417
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 418
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 418
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 419
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 419
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 420
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 420
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 421
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 421
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 422
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 422
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 423
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 423
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 424
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 424
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 425
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 425
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 426
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 426
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 427
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 427
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 428
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 428
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 429
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 429
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 430
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 430
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 431
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 431
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 432
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 432
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 433
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 433
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 434
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 434
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 435
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 435
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 436
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 436
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 437
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 437
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 438
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 438
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 439
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 439
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 440
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 440
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 441
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 441
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgcgtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 442
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 442
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Arg Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 443
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 443
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 444
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 444
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 445
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 445
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 446
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 446
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 447
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 447
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 448
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 448
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 449
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 449
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 450
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 450
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 451
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 451
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 452
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 452
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 453
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 453
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggtcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 454
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 454
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 455
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 455
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 456
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 456
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 457
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 457
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 458
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 458
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 459
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 459
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 460
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 460
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 461
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 461
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 462
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 462
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 463
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 463
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 464
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 464
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 465
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 465
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 466
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 466
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 467
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 467
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 468
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 468
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 469
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 469
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 470
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 470
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 471
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 471
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 472
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 472
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 473
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 473
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggtcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 474
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 474
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 475
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 475
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggtcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 476
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 476
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 477
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 477
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 478
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 478
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 479
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 479
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattggggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 480
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 480
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Gly Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 481
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 481
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattggggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 482
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 482
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Gly Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 483
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 483
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggtcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gccgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 484
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 484
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Val Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 485
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 485
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccagtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc ggggggcccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 486
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 486
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Ser Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 487
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 487
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtact acgattctag atcagctggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
gtggacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 488
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 488
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Val Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 489
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 489
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcgtgaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 490
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 490
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Arg Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 491
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 491
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
aagaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 492
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 492
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Lys Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 493
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 493
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 494
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 494
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 495
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 495
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatagg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 496
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 496
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Arg Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 497
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 497
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
acgaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 498
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 498
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 499
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 499
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctggcg 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 500
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 500
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ala Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 501
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 501
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
tcggtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 502
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 502
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Ser Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 503
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 503
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggcca gggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 504
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 504
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Gln Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 505
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 505
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgg ctccggtact acgattctag atcagttggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 506
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 506
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Gly Ser Gly Thr Thr Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 507
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 507
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatttgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 508
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 508
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 509
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 509
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcagt 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 510
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 510
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Ser Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 511
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 511
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 512
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 512
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Glu Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 513
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 513
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 514
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 514
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 515
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 515
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcag ggagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 516
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 516
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Arg Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 517
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 517
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 518
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 518
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 519
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 519
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgagggaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 520
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 520
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Arg 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 521
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 521
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccatgatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 522
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 522
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Met Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 523
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 523
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagtcg 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 524
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 524
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu Ser 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 525
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 525
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatgtt 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 526
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 526
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Val Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 527
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 527
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgaatgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcaggaggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgcggct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgtttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact gtggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 528
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 528
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Asn 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Glu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Val Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 529
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 529
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga gggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatctg 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 530
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 530
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Glu Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 531
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 531
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga tggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctggcg 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcgttgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccacgatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacg 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 532
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 532
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ala Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Arg Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 533
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 533
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga tggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatcgt 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
agcgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttctcg 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 534
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 534
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Arg Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Ser Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Ser Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 535
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 535
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga tggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccacgatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 536
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 536
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 537
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 537
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgcgcgaga tgggatattt taaggatctg 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccacgatccg tgagatgaaa gaagcgggtg ataacaccat cgtcttcacg 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 538
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 538
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Arg 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Arg Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 539
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 539
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatcgt 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 540
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 540
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Arg Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 541
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 541
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga tggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tgacgggtgt gggtaaaatt 780
atcgacatct acgcgggccg tggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacg 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 542
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 542
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Thr Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 543
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 543
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctggcg 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt taaggatctg 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 544
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 544
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ala Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         

 
<210> 545
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 545
atgaaacgtg catttattat gacgatggac tcattcggca tcggtgctac agaagatgca 60
gaaaagtttg gtgacgtagg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatgtgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcaa gtggtaaaga tagcctgtcg 300
ggtcactggg aaattgccgg tgtcccggtt ctgatggaga tgggatattt taaggatctg 360
actaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc ctccggtact acgattctag atcagtgggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgaagcat ccgtgttcca gattgccgct 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggttacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac tgggaaccgt cgtgacctgg ctcaagagcc gggggggccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggttggtgt gggtaaaatt 780
atcgacatct acgcgggccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgaca ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc tgtgtggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgg cgctgctgcg cgatgacgac 1020
attctgatcc tcaccgctga ccacggttgc gatccgacct ggaaaggtga agaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 546
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 546
Met Lys Arg Ala Phe Ile Met Thr Met Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Lys Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Val Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Met 
            100                 105                 110         
Glu Met Gly Tyr Phe Lys Asp Leu Thr Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Thr Ile Leu Asp Gln Trp Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Glu Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Ala His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Gln Glu Pro Gly Gly Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Gly 
                245                 250                 255     
Val Gly Lys Ile Ile Asp Ile Tyr Ala Gly Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Thr Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Val Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ala Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Lys Gly Glu Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405         


<210> 547
<211> 771
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis
 
<400> 547
atgtgcgatc tgaagaaggc agcgcagcgg gcgattagcc tgatggatct gaccaccctg 60
aacgacgatg acaccgacca gaaagtgatt gagctgtgcc acaaagcgaa aaccccggcg 120
ggtgacaccg cggcgatcgt gatttacccg cgtttcatcc cgattgcgcg taagaccctg 180
aacgaaatcg gtggcctgga catcaaaatt gccaccgtta ccaactttcc gcacggtaac 240
gatgacattg cgattgcggt gctggagacc cgtgcggcgg ttgcgtacgg cgcggatgaa 300
gtggacgtgg ttttcccgta tcgtgcgctg atggaaggta acgaaaccgt gggctttgag 360
ctggttaagg cgtgcaaaga ggcgtgcggt gaagatcata ttctgaaggt gatcattgaa 420
agtggcgtac tgaaagaccc ggcgctgatt cgtaaagcga gcgaaattag catcgatgcg 480
ggtgcggact tcattaagac cagcaccggc aaagttgcgg ttaacgcgac cctggaggcg 540
gcggaaatca ttatgaccgt tattagcgag aagaacccga aagtgggttt caagccggcg 600
ggtggcatta aagatgctgc ggcggcggcg gaatttctgg gtgtggcggc gcgtctgctg 660
ggcgatgact gggcgacccc ggcgaccttc cgttttggtg cgaccgacct gctgaccaac 720
ctgctgcaca ccctggaact ggcggacgcg ccgcagggtg cgcaaggcta t          771

 
<210> 548
<211> 257
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis
 
<400> 548
Met Cys Asp Leu Lys Lys Ala Ala Gln Arg Ala Ile Ser Leu Met Asp 
1               5                   10                  15      
Leu Thr Thr Leu Asn Asp Asp Asp Thr Asp Gln Lys Val Ile Glu Leu 
            20                  25                  30          
Cys His Lys Ala Lys Thr Pro Ala Gly Asp Thr Ala Ala Ile Val Ile 
        35                  40                  45              
Tyr Pro Arg Phe Ile Pro Ile Ala Arg Lys Thr Leu Asn Glu Ile Gly 
    50                  55                  60                  
Gly Leu Asp Ile Lys Ile Ala Thr Val Thr Asn Phe Pro His Gly Asn 
65                  70                  75                  80  
Asp Asp Ile Ala Ile Ala Val Leu Glu Thr Arg Ala Ala Val Ala Tyr 
                85                  90                  95      
Gly Ala Asp Glu Val Asp Val Val Phe Pro Tyr Arg Ala Leu Met Glu 
            100                 105                 110         
Gly Asn Glu Thr Val Gly Phe Glu Leu Val Lys Ala Cys Lys Glu Ala 
        115                 120                 125             
Cys Gly Glu Asp His Ile Leu Lys Val Ile Ile Glu Ser Gly Val Leu 
    130                 135                 140                 
Lys Asp Pro Ala Leu Ile Arg Lys Ala Ser Glu Ile Ser Ile Asp Ala 
145                 150                 155                 160 
Gly Ala Asp Phe Ile Lys Thr Ser Thr Gly Lys Val Ala Val Asn Ala 
                165                 170                 175     
Thr Leu Glu Ala Ala Glu Ile Ile Met Thr Val Ile Ser Glu Lys Asn 
            180                 185                 190         
Pro Lys Val Gly Phe Lys Pro Ala Gly Gly Ile Lys Asp Ala Ala Ala 
        195                 200                 205             
Ala Ala Glu Phe Leu Gly Val Ala Ala Arg Leu Leu Gly Asp Asp Trp 
    210                 215                 220                 
Ala Thr Pro Ala Thr Phe Arg Phe Gly Ala Thr Asp Leu Leu Thr Asn 
225                 230                 235                 240 
Leu Leu His Thr Leu Glu Leu Ala Asp Ala Pro Gln Gly Ala Gln Gly 
                245                 250                 255     
Tyr                                                             
                                                                

<210> 549
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 549
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 550
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 550
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 551
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 551
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 552
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 552
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
