                         SEQUENCE LISTING

<110>  Virginia Commonwealth University
 
<120>  A FACTOR H BINDING PROTEIN B (FhbB) BASED CHIMERIC VACCINE FOR 
       THE PREVENTION AND TREATMENT OF PERIODONTAL DISEASE

<130>  02941479TA

<150>  US 63/087,389
<151>  2020-10-05

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant protein

<400>  1

Thr Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Lys Phe Ile 
1               5                   10                  15      


Asn Ala Leu Glu Asn Ala Leu Lys Thr Arg His Ile Pro Ala Gly Ala 
            20                  25                  30          


Val Ile Asp Met Leu Ala Glu Ile Asn Thr Glu Ala Leu Ala Leu Asp 
        35                  40                  45              


Tyr Gln Ile Val Asp Lys Lys Pro Gly Thr Ser Ile Ala Gln Gly Thr 
    50                  55                  60                  


Lys Ala Ala Ala Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys Ala 
65                  70                  75                  80  


<210>  2
<211>  81
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant protein

<400>  2

Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Lys 
1               5                   10                  15      


Val Leu Glu Lys Ala Ala Glu Arg Asn Pro Ile Asp Ala Gln Val Val 
            20                  25                  30          


Val Glu Ala Leu Gly Ala Val Asn Ile Asp Ala Leu Ala Lys Asn Leu 
        35                  40                  45              


Asn Tyr Gln Val Ile Asp Lys Lys Pro Gly Thr Asp Ile Ala Thr Gly 
    50                  55                  60                  


Thr Lys Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Ile Lys 
65                  70                  75                  80  


Ala 
    


<210>  3
<211>  78
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant protein

<400>  3

Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Ser 
1               5                   10                  15      


Gly Leu Glu Asn Ala Val Lys Asp Asn Pro Met Thr Ala Gln Asn Val 
            20                  25                  30          


Lys Glu Gly Leu Asp Leu Ala Asn Val Gly Ala Ala Ala Leu Asn Phe 
        35                  40                  45              


Lys Ile Val Asp Lys Lys Ala Gly Thr Glu Ile Ala Lys Gly Thr Lys 
    50                  55                  60                  


Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Lys Ala 
65                  70                  75              


<210>  4
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant protein

<400>  4

Phe Lys Met Asn Thr Ala Gln Asn Ala His Tyr Glu Ala Phe Ile Ser 
1               5                   10                  15      


Gly Leu Glu Arg Gly Ala Lys Asp Asn Pro Met Leu Ala Gln Val Val 
            20                  25                  30          


Lys Ala Gly Leu Asp Leu Ala Asn Asp Gly Ala Ala Ala Leu Asn Tyr 
        35                  40                  45              


Lys Ile Val Asp Lys Lys Pro Gly Thr Asp Ile Ala Lys Gly Thr Lys 
    50                  55                  60                  


Ala Ala Glu Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys Thr 
65                  70                  75                  


<210>  5
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic mutant protein

<400>  5

Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Ala 
1               5                   10                  15      


Asp Leu Glu Arg Ala Ala Lys Asp Asn Pro Met Pro Ala His Ile Val 
            20                  25                  30          


Lys Ala Gly Leu Asp Ala Ala Asn Ala Ile Ala Ala Thr Leu Asn Phe 
        35                  40                  45              


Lys Ile Val Asp Lys Lys Ala Gly Thr Glu Ile Ala Lys Gly Thr Lys 
    50                  55                  60                  


Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Lys 
65                  70                  75          


<210>  6
<211>  395
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic chimeric protein

<400>  6

Thr Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Lys Phe Ile 
1               5                   10                  15      


Asn Ala Leu Glu Asn Ala Leu Lys Thr Arg His Ile Pro Ala Gly Ala 
            20                  25                  30          


Val Ile Asp Met Leu Ala Glu Ile Asn Thr Glu Ala Leu Ala Leu Asp 
        35                  40                  45              


Tyr Gln Ile Val Asp Lys Lys Pro Gly Thr Ser Ile Ala Gln Gly Thr 
    50                  55                  60                  


Lys Ala Ala Ala Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys Ala 
65                  70                  75                  80  


Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Lys 
                85                  90                  95      


Val Leu Glu Lys Ala Ala Glu Arg Asn Pro Ile Asp Ala Gln Val Val 
            100                 105                 110         


Val Glu Ala Leu Gly Ala Val Asn Ile Asp Ala Leu Ala Lys Asn Leu 
        115                 120                 125             


Asn Tyr Gln Val Ile Asp Lys Lys Pro Gly Thr Asp Ile Ala Thr Gly 
    130                 135                 140                 


Thr Lys Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Ile Lys 
145                 150                 155                 160 


Ala Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile 
                165                 170                 175     


Ser Gly Leu Glu Asn Ala Val Lys Asp Asn Pro Met Thr Ala Gln Asn 
            180                 185                 190         


Val Lys Glu Gly Leu Asp Leu Ala Asn Val Gly Ala Ala Ala Leu Asn 
        195                 200                 205             


Phe Lys Ile Val Asp Lys Lys Ala Gly Thr Glu Ile Ala Lys Gly Thr 
    210                 215                 220                 


Lys Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Lys Ala Phe 
225                 230                 235                 240 


Lys Met Asn Thr Ala Gln Asn Ala His Tyr Glu Ala Phe Ile Ser Gly 
                245                 250                 255     


Leu Glu Arg Gly Ala Lys Asp Asn Pro Met Leu Ala Gln Val Val Lys 
            260                 265                 270         


Ala Gly Leu Asp Leu Ala Asn Asp Gly Ala Ala Ala Leu Asn Tyr Lys 
        275                 280                 285             


Ile Val Asp Lys Lys Pro Gly Thr Asp Ile Ala Lys Gly Thr Lys Ala 
    290                 295                 300                 


Ala Glu Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys Thr Phe Lys 
305                 310                 315                 320 


Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Ala Asp Leu 
                325                 330                 335     


Glu Arg Ala Ala Lys Asp Asn Pro Met Pro Ala His Ile Val Lys Ala 
            340                 345                 350         


Gly Leu Asp Ala Ala Asn Ala Ile Ala Ala Thr Leu Asn Phe Lys Ile 
        355                 360                 365             


Val Asp Lys Lys Ala Gly Thr Glu Ile Ala Lys Gly Thr Lys Ala Ala 
    370                 375                 380                 


Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Lys 
385                 390                 395 


<210>  7
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic chimeric protein

<400>  7

Thr Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Lys Phe Ile 
1               5                   10                  15      


Asn Ala Leu Glu Asn Ala Leu Lys Thr Arg His Ile Pro Ala Gly Ala 
            20                  25                  30          


Val Ile Asp Met Leu Ala Glu Ile Asn Thr Glu Ala Leu Ala Leu Asp 
        35                  40                  45              


Tyr Gln Ile Val Asp Lys Lys Pro Gly Thr Ser Ile Ala Gln Gly Thr 
    50                  55                  60                  


Lys Ala Ala Ala Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys Ala 
65                  70                  75                  80  


Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile Lys 
                85                  90                  95      


Val Leu Glu Lys Ala Ala Glu Arg Asn Pro Ile Asp Ala Gln Val Val 
            100                 105                 110         


Val Glu Ala Leu Gly Ala Val Asn Ile Asp Ala Leu Ala Lys Asn Leu 
        115                 120                 125             


Asn Tyr Gln Val Ile Asp Lys Lys Pro Gly Thr Asp Ile Ala Thr Gly 
    130                 135                 140                 


Thr Lys Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Ile Lys 
145                 150                 155                 160 


Ala Phe Lys Met Asn Thr Ala Gln Lys Ala His Tyr Glu Ala Phe Ile 
                165                 170                 175     


Ser Gly Leu Glu Asn Ala Val Lys Asp Asn Pro Met Thr Ala Gln Asn 
            180                 185                 190         


Val Lys Glu Gly Leu Asp Leu Ala Asn Val Gly Ala Ala Ala Leu Asn 
        195                 200                 205             


Phe Lys Ile Val Asp Lys Lys Ala Gly Thr Glu Ile Ala Lys Gly Thr 
    210                 215                 220                 


Lys Ala Ala Glu Leu Arg Lys Arg Phe Val Pro Lys Lys Lys Ala Phe 
225                 230                 235                 240 


Lys Met Asn Thr Ala Gln Asn Ala His Tyr Glu Ala Phe Ile Ser Gly 
                245                 250                 255     


Leu Glu Arg Gly Ala Lys Asp Asn Pro Met Leu Ala Gln Val Val Lys 
            260                 265                 270         


Ala Gly Leu Asp Leu Ala Asn Asp Gly Ala Ala Ala Leu Asn Tyr Lys 
        275                 280                 285             


Ile Val Asp Lys Lys Pro Gly Thr Asp Ile Ala Lys Gly Thr Lys Ala 
    290                 295                 300                 


Ala Glu Leu Arg Lys Arg Phe Ile Pro Lys Lys Ile Lys 
305                 310                 315         


<210>  8
<211>  1185
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic coding sequence for chimeric protein

<400>  8
accttcaaaa tgaataccgc gcagaaggcc cattatgaga agttcatcaa tgccctggag       60

aacgccctga aaacccgcca tatccctgct ggtgccgtta tcgacatgct ggccgagatt      120

aacaccgagg ccctggcact ggactatcag atcgtggata aaaaaccggg caccagcatt      180

gcacagggta ccaaggccgc cgcactgcgt aaacgtttta ttcctaagaa aattaaagca      240

ttcaagatga ataccgcaca gaaagcacat tacgaagcat tcattaaagt gctggagaag      300

gccgccgagc gcaacccgat tgacgcacag gttgttgttg aagcactggg cgccgttaac      360

atcgacgccc tggcaaaaaa cctgaactat caggtgattg acaagaagcc gggcaccgat      420

attgccaccg gtaccaaggc cgcagagctg cgcaagcgct tcgtgccgaa gaaaattaaa      480

gcctttaaaa tgaacaccgc ccagaaagcc cattacgagg cattcatcag cggtctggaa      540

aatgccgtga aggataatcc gatgaccgca cagaacgtta aagaaggcct ggacctggca      600

aatgtgggcg ccgcagccct gaactttaag attgtggata agaaagcagg taccgagatt      660

gccaagggca ccaaagccgc agaactgcgc aaacgctttg tgccgaagaa aaaagccttt      720

aagatgaata ccgcccagaa cgcccactac gaagcattta ttagcggtct ggagcgcggt      780

gccaaagata acccgatgct ggcacaggtt gtgaaggccg gcctggatct ggccaatgat      840

ggtgccgccg cactgaacta caaaattgtg gataaaaagc cgggcaccga catcgccaaa      900

ggtaccaaag ccgccgaact gcgcaaacgt ttcattccga agaaaattaa aaccttcaaa      960

atgaataccg cccaaaaggc acactatgag gcattcattg ccgatctgga acgcgccgcc     1020

aaggacaatc ctatgccggc ccatattgtg aaagcaggtc tggatgccgc caatgcaatc     1080

gccgccaccc tgaatttcaa gatcgtggac aagaaggccg gcacagaaat cgccaaaggc     1140

accaaggccg cagaactgcg caagcgcttt gtgccgaaaa agaaa                     1185


<210>  9
<211>  1185
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic codon optimized coding sequence for chimeric protein

<400>  9
acatttaaga tgaacaccgc ccaaaaggcc cattacgaga aattcatcaa cgccctggaa       60

aacgccctga agacccgtca tattcctgct ggtgccgtga ttgatatgct ggccgagatt      120

aacaccgaag ccctggccct ggactaccag atcgtggata agaaaccggg caccagtatt      180

gcccaaggta ccaaggccgc cgcactgcgc aagcgcttta tcccgaaaaa gattaaagcc      240

ttcaagatga acaccgccca aaaagcccat tacgaggcct tcattaaagt gctggaaaaa      300

gccgccgagc gtaatccgat cgatgcacag gtggtggttg aggccctggg cgcagtgaat      360

attgacgccc tggcaaaaaa tctgaactac caggtgatcg acaaaaagcc gggcaccgac      420

attgccaccg gtaccaaagc agcagaactg cgcaaacgct ttgtgccgaa gaaaattaag      480

gccttcaaaa tgaacaccgc ccagaaagcc cattatgagg cattcattag cggcctggag      540

aacgccgtta aagacaaccc gatgacagcc cagaacgtga aagaaggtct ggacctggcc      600

aatgtgggcg cagcagccct gaatttcaaa attgtggata agaaggccgg caccgagatt      660

gccaaaggca ccaaggccgc cgagctgcgc aagcgcttcg tgccgaagaa aaaagccttt      720

aagatgaata ccgcacagaa cgcccattac gaagccttca tcagcggtct ggaacgtggc      780

gcaaaggata acccgatgct ggcccaggtt gttaaagccg gtctggatct ggcaaacgat      840

ggcgccgccg cactgaacta taaaatcgtg gacaagaagc cgggtaccga tattgccaag      900

ggcaccaaag cagccgaact gcgtaaacgc ttcatcccga agaagattaa aacctttaaa      960

atgaacaccg cacaaaaggc ccactacgaa gcctttatcg ccgatctgga acgtgcagcc     1020

aaagacaatc cgatgccggc ccacattgtt aaggccggtc tggacgcagc aaacgccatc     1080

gccgccaccc tgaactttaa aatcgtggac aaaaaggccg gtaccgaaat tgccaagggc     1140

accaaggccg ccgagctgcg caaacgtttt gttccgaaaa agaaa                     1185


<210>  10
<211>  960
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetid coding sequence for chimeric protein

<400>  10
accttcaaaa tgaataccgc gcagaaggcc cattatgaga agttcatcaa tgccctggag       60

aacgccctga aaacccgcca tatccctgct ggtgccgtta tcgacatgct ggccgagatt      120

aacaccgagg ccctggcact ggactatcag atcgtggata aaaaaccggg caccagcatt      180

gcacagggta ccaaggccgc cgcactgcgt aaacgtttta ttcctaagaa aattaaagca      240

ttcaagatga ataccgcaca gaaagcacat tacgaagcat tcattaaagt gctggagaag      300

gccgccgagc gcaacccgat tgacgcacag gttgttgttg aagcactggg cgccgttaac      360

atcgacgccc tggcaaaaaa cctgaactat caggtgattg acaagaagcc gggcaccgat      420

attgccaccg gtaccaaggc cgcagagctg cgcaagcgct tcgtgccgaa gaaaattaaa      480

gcctttaaaa tgaacaccgc ccagaaagcc cattacgagg cattcatcag cggtctggaa      540

aatgccgtga aggataatcc gatgaccgca cagaacgtta aagaaggcct ggacctggca      600

aatgtgggcg ccgcagccct gaactttaag attgtggata agaaagcagg taccgagatt      660

gccaagggca ccaaagccgc agaactgcgc aaacgctttg tgccgaagaa aaaagccttt      720

aagatgaata ccgcccagaa cgcccactac gaagcattta ttagcggtct ggagcgcggt      780

gccaaagata acccgatgct ggcacaggtt gtgaaggccg gcctggatct ggccaatgat      840

ggtgccgccg cactgaacta caaaattgtg gataaaaagc cgggcaccga catcgccaaa      900

ggtaccaaag ccgccgaact gcgcaaacgt ttcattccga agaaaattaa aaccttcaaa      960


<210>  11
<211>  960
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic codon optimized coding sequence for chimeric protein

<400>  11
acatttaaga tgaacaccgc ccaaaaggcc cattacgaga aattcatcaa cgccctggaa       60

aacgccctga agacccgtca tattcctgct ggtgccgtga ttgatatgct ggccgagatt      120

aacaccgaag ccctggccct ggactaccag atcgtggata agaaaccggg caccagtatt      180

gcccaaggta ccaaggccgc cgcactgcgc aagcgcttta tcccgaaaaa gattaaagcc      240

ttcaagatga acaccgccca aaaagcccat tacgaggcct tcattaaagt gctggaaaaa      300

gccgccgagc gtaatccgat cgatgcacag gtggtggttg aggccctggg cgcagtgaat      360

attgacgccc tggcaaaaaa tctgaactac caggtgatcg acaaaaagcc gggcaccgac      420

attgccaccg gtaccaaagc agcagaactg cgcaaacgct ttgtgccgaa gaaaattaag      480

gccttcaaaa tgaacaccgc ccagaaagcc cattatgagg cattcattag cggcctggag      540

aacgccgtta aagacaaccc gatgacagcc cagaacgtga aagaaggtct ggacctggcc      600

aatgtgggcg cagcagccct gaatttcaaa attgtggata agaaggccgg caccgagatt      660

gccaaaggca ccaaggccgc cgagctgcgc aagcgcttcg tgccgaagaa aaaagccttt      720

aagatgaata ccgcacagaa cgcccattac gaagccttca tcagcggtct ggaacgtggc      780

gcaaaggata acccgatgct ggcccaggtt gttaaagccg gtctggatct ggcaaacgat      840

ggcgccgccg cactgaacta taaaatcgtg gacaagaagc cgggtaccga tattgccaag      900

ggcaccaaag cagccgaact gcgtaaacgc ttcatcccga agaagattaa aacctttaaa      960


