                         SEQUENCE LISTING

<110>  OXFORD NANOPORE TECHNOLOGIES LIMITED
 
<120>  METHOD

<130>  N420382WO

<150>  GB 2015993.5
<151>  2020-10-08

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  485
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Met Asn Asp Gly Lys Gln Gln Ser Thr Phe Leu Phe His Asp Tyr 
1               5                   10                  15      


Glu Thr Phe Gly Thr His Pro Ala Leu Asp Arg Pro Ala Gln Phe Ala 
            20                  25                  30          


Ala Ile Arg Thr Asp Ser Glu Phe Asn Val Ile Gly Glu Pro Glu Val 
        35                  40                  45              


Phe Tyr Cys Lys Pro Ala Asp Asp Tyr Leu Pro Gln Pro Gly Ala Val 
    50                  55                  60                  


Leu Ile Thr Gly Ile Thr Pro Gln Glu Ala Arg Ala Lys Gly Glu Asn 
65                  70                  75                  80  


Glu Ala Ala Phe Ala Ala Arg Ile His Ser Leu Phe Thr Val Pro Lys 
                85                  90                  95      


Thr Cys Ile Leu Gly Tyr Asn Asn Val Arg Phe Asp Asp Glu Val Thr 
            100                 105                 110         


Arg Asn Ile Phe Tyr Arg Asn Phe Tyr Asp Pro Tyr Ala Trp Ser Trp 
        115                 120                 125             


Gln His Asp Asn Ser Arg Trp Asp Leu Leu Asp Val Met Arg Ala Cys 
    130                 135                 140                 


Tyr Ala Leu Arg Pro Glu Gly Ile Asn Trp Pro Glu Asn Asp Asp Gly 
145                 150                 155                 160 


Leu Pro Ser Phe Arg Leu Glu His Leu Thr Lys Ala Asn Gly Ile Glu 
                165                 170                 175     


His Ser Asn Ala His Asp Ala Met Ala Asp Val Tyr Ala Thr Ile Ala 
            180                 185                 190         


Met Ala Lys Leu Val Lys Thr Arg Gln Pro Arg Leu Phe Asp Tyr Leu 
        195                 200                 205             


Phe Thr His Arg Asn Lys His Lys Leu Met Ala Leu Ile Asp Val Pro 
    210                 215                 220                 


Gln Met Lys Pro Leu Val His Val Ser Gly Met Phe Gly Ala Trp Arg 
225                 230                 235                 240 


Gly Asn Thr Ser Trp Val Ala Pro Leu Ala Trp His Pro Glu Asn Arg 
                245                 250                 255     


Asn Ala Val Ile Met Val Asp Leu Ala Gly Asp Ile Ser Pro Leu Leu 
            260                 265                 270         


Glu Leu Asp Ser Asp Thr Leu Arg Glu Arg Leu Tyr Thr Ala Lys Thr 
        275                 280                 285             


Asp Leu Gly Asp Asn Ala Ala Val Pro Val Lys Leu Val His Ile Asn 
    290                 295                 300                 


Lys Cys Pro Val Leu Ala Gln Ala Asn Thr Leu Arg Pro Glu Asp Ala 
305                 310                 315                 320 


Asp Arg Leu Gly Ile Asn Arg Gln His Cys Leu Asp Asn Leu Lys Ile 
                325                 330                 335     


Leu Arg Glu Asn Pro Gln Val Arg Glu Lys Val Val Ala Ile Phe Ala 
            340                 345                 350         


Glu Ala Glu Pro Phe Thr Pro Ser Asp Asn Val Asp Ala Gln Leu Tyr 
        355                 360                 365             


Asn Gly Phe Phe Ser Asp Ala Asp Arg Ala Ala Met Lys Ile Val Leu 
    370                 375                 380                 


Glu Thr Glu Pro Arg Asn Leu Pro Ala Leu Asp Ile Thr Phe Val Asp 
385                 390                 395                 400 


Lys Arg Ile Glu Lys Leu Leu Phe Asn Tyr Arg Ala Arg Asn Phe Pro 
                405                 410                 415     


Gly Thr Leu Asp Tyr Ala Glu Gln Gln Arg Trp Leu Glu His Arg Arg 
            420                 425                 430         


Gln Val Phe Thr Pro Glu Phe Leu Gln Gly Tyr Ala Asp Glu Leu Gln 
        435                 440                 445             


Met Leu Val Gln Gln Tyr Ala Asp Asp Lys Glu Lys Val Ala Leu Leu 
    450                 455                 460                 


Lys Ala Leu Trp Gln Tyr Ala Glu Glu Ile Val Ser Gly Ser Gly His 
465                 470                 475                 480 


His His His His His 
                485 


<210>  2
<211>  268
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Lys Phe Val Ser Phe Asn Ile Asn Gly Leu Arg Ala Arg Pro His 
1               5                   10                  15      


Gln Leu Glu Ala Ile Val Glu Lys His Gln Pro Asp Val Ile Gly Leu 
            20                  25                  30          


Gln Glu Thr Lys Val His Asp Asp Met Phe Pro Leu Glu Glu Val Ala 
        35                  40                  45              


Lys Leu Gly Tyr Asn Val Phe Tyr His Gly Gln Lys Gly His Tyr Gly 
    50                  55                  60                  


Val Ala Leu Leu Thr Lys Glu Thr Pro Ile Ala Val Arg Arg Gly Phe 
65                  70                  75                  80  


Pro Gly Asp Asp Glu Glu Ala Gln Arg Arg Ile Ile Met Ala Glu Ile 
                85                  90                  95      


Pro Ser Leu Leu Gly Asn Val Thr Val Ile Asn Gly Tyr Phe Pro Gln 
            100                 105                 110         


Gly Glu Ser Arg Asp His Pro Ile Lys Phe Pro Ala Lys Ala Gln Phe 
        115                 120                 125             


Tyr Gln Asn Leu Gln Asn Tyr Leu Glu Thr Glu Leu Lys Arg Asp Asn 
    130                 135                 140                 


Pro Val Leu Ile Met Gly Asp Met Asn Ile Ser Pro Thr Asp Leu Asp 
145                 150                 155                 160 


Ile Gly Ile Gly Glu Glu Asn Arg Lys Arg Trp Leu Arg Thr Gly Lys 
                165                 170                 175     


Cys Ser Phe Leu Pro Glu Glu Arg Glu Trp Met Asp Arg Leu Met Ser 
            180                 185                 190         


Trp Gly Leu Val Asp Thr Phe Arg His Ala Asn Pro Gln Thr Ala Asp 
        195                 200                 205             


Arg Phe Ser Trp Phe Asp Tyr Arg Ser Lys Gly Phe Asp Asp Asn Arg 
    210                 215                 220                 


Gly Leu Arg Ile Asp Leu Leu Leu Ala Ser Gln Pro Leu Ala Glu Cys 
225                 230                 235                 240 


Cys Val Glu Thr Gly Ile Asp Tyr Glu Ile Arg Ser Met Glu Lys Pro 
                245                 250                 255     


Ser Asp His Ala Pro Val Trp Ala Thr Phe Arg Arg 
            260                 265             


<210>  3
<211>  425
<212>  PRT
<213>  Thermus thermophilus

<400>  3

Met Phe Arg Arg Lys Glu Asp Leu Asp Pro Pro Leu Ala Leu Leu Pro 
1               5                   10                  15      


Leu Lys Gly Leu Arg Glu Ala Ala Ala Leu Leu Glu Glu Ala Leu Arg 
            20                  25                  30          


Gln Gly Lys Arg Ile Arg Val His Gly Asp Tyr Asp Ala Asp Gly Leu 
        35                  40                  45              


Thr Gly Thr Ala Ile Leu Val Arg Gly Leu Ala Ala Leu Gly Ala Asp 
    50                  55                  60                  


Val His Pro Phe Ile Pro His Arg Leu Glu Glu Gly Tyr Gly Val Leu 
65                  70                  75                  80  


Met Glu Arg Val Pro Glu His Leu Glu Ala Ser Asp Leu Phe Leu Thr 
                85                  90                  95      


Val Asp Cys Gly Ile Thr Asn His Ala Glu Leu Arg Glu Leu Leu Glu 
            100                 105                 110         


Asn Gly Val Glu Val Ile Val Thr Asp His His Thr Pro Gly Lys Thr 
        115                 120                 125             


Pro Pro Pro Gly Leu Val Val His Pro Ala Leu Thr Pro Asp Leu Lys 
    130                 135                 140                 


Glu Lys Pro Thr Gly Ala Gly Val Ala Phe Leu Leu Leu Trp Ala Leu 
145                 150                 155                 160 


His Glu Arg Leu Gly Leu Pro Pro Pro Leu Glu Tyr Ala Asp Leu Ala 
                165                 170                 175     


Ala Val Gly Thr Ile Ala Asp Val Ala Pro Leu Trp Gly Trp Asn Arg 
            180                 185                 190         


Ala Leu Val Lys Glu Gly Leu Ala Arg Ile Pro Ala Ser Ser Trp Val 
        195                 200                 205             


Gly Leu Arg Leu Leu Ala Glu Ala Val Gly Tyr Thr Gly Lys Ala Val 
    210                 215                 220                 


Glu Val Ala Phe Arg Ile Ala Pro Arg Ile Asn Ala Ala Ser Arg Leu 
225                 230                 235                 240 


Gly Glu Ala Glu Lys Ala Leu Arg Leu Leu Leu Thr Asp Asp Ala Ala 
                245                 250                 255     


Glu Ala Gln Ala Leu Val Gly Glu Leu His Arg Leu Asn Ala Arg Arg 
            260                 265                 270         


Gln Thr Leu Glu Glu Ala Met Leu Arg Lys Leu Leu Pro Gln Ala Asp 
        275                 280                 285             


Pro Glu Ala Lys Ala Ile Val Leu Leu Asp Pro Glu Gly His Pro Gly 
    290                 295                 300                 


Val Met Gly Ile Val Ala Ser Arg Ile Leu Glu Ala Thr Leu Arg Pro 
305                 310                 315                 320 


Val Phe Leu Val Ala Gln Gly Lys Gly Thr Val Arg Ser Leu Ala Pro 
                325                 330                 335     


Ile Ser Ala Val Glu Ala Leu Arg Ser Ala Glu Asp Leu Leu Leu Arg 
            340                 345                 350         


Tyr Gly Gly His Lys Glu Ala Ala Gly Phe Ala Met Asp Glu Ala Leu 
        355                 360                 365             


Phe Pro Ala Phe Lys Ala Arg Val Glu Ala Tyr Ala Ala Arg Phe Pro 
    370                 375                 380                 


Asp Pro Val Arg Glu Val Ala Leu Leu Asp Leu Leu Pro Glu Pro Gly 
385                 390                 395                 400 


Leu Leu Pro Gln Val Phe Arg Glu Leu Ala Leu Leu Glu Pro Tyr Gly 
                405                 410                 415     


Glu Gly Asn Pro Glu Pro Leu Phe Leu 
            420                 425 


<210>  4
<211>  226
<212>  PRT
<213>  Bacteriophage lambda

<400>  4

Met Thr Pro Asp Ile Ile Leu Gln Arg Thr Gly Ile Asp Val Arg Ala 
1               5                   10                  15      


Val Glu Gln Gly Asp Asp Ala Trp His Lys Leu Arg Leu Gly Val Ile 
            20                  25                  30          


Thr Ala Ser Glu Val His Asn Val Ile Ala Lys Pro Arg Ser Gly Lys 
        35                  40                  45              


Lys Trp Pro Asp Met Lys Met Ser Tyr Phe His Thr Leu Leu Ala Glu 
    50                  55                  60                  


Val Cys Thr Gly Val Ala Pro Glu Val Asn Ala Lys Ala Leu Ala Trp 
65                  70                  75                  80  


Gly Lys Gln Tyr Glu Asn Asp Ala Arg Thr Leu Phe Glu Phe Thr Ser 
                85                  90                  95      


Gly Val Asn Val Thr Glu Ser Pro Ile Ile Tyr Arg Asp Glu Ser Met 
            100                 105                 110         


Arg Thr Ala Cys Ser Pro Asp Gly Leu Cys Ser Asp Gly Asn Gly Leu 
        115                 120                 125             


Glu Leu Lys Cys Pro Phe Thr Ser Arg Asp Phe Met Lys Phe Arg Leu 
    130                 135                 140                 


Gly Gly Phe Glu Ala Ile Lys Ser Ala Tyr Met Ala Gln Val Gln Tyr 
145                 150                 155                 160 


Ser Met Trp Val Thr Arg Lys Asn Ala Trp Tyr Phe Ala Asn Tyr Asp 
                165                 170                 175     


Pro Arg Met Lys Arg Glu Gly Leu His Tyr Val Val Ile Glu Arg Asp 
            180                 185                 190         


Glu Lys Tyr Met Ala Ser Phe Asp Glu Ile Val Pro Glu Phe Ile Glu 
        195                 200                 205             


Lys Met Asp Glu Ala Leu Ala Glu Ile Gly Phe Val Phe Gly Glu Gln 
    210                 215                 220                 


Trp Arg 
225     


<210>  5
<211>  608
<212>  PRT
<213>  Bacillus subtilis

<400>  5

Met Lys His Met Pro Arg Lys Met Tyr Ser Cys Ala Phe Glu Thr Thr 
1               5                   10                  15      


Thr Lys Val Glu Asp Cys Arg Val Trp Ala Tyr Gly Tyr Met Asn Ile 
            20                  25                  30          


Glu Asp His Ser Glu Tyr Lys Ile Gly Asn Ser Leu Asp Glu Phe Met 
        35                  40                  45              


Ala Trp Val Leu Lys Val Gln Ala Asp Leu Tyr Phe His Asn Leu Lys 
    50                  55                  60                  


Phe Asp Gly Ala Phe Ile Ile Asn Trp Leu Glu Arg Asn Gly Phe Lys 
65                  70                  75                  80  


Trp Ser Ala Asp Gly Leu Pro Asn Thr Tyr Asn Thr Ile Ile Ser Arg 
                85                  90                  95      


Met Gly Gln Trp Tyr Met Ile Asp Ile Cys Leu Gly Tyr Lys Gly Lys 
            100                 105                 110         


Arg Lys Ile His Thr Val Ile Tyr Asp Ser Leu Lys Lys Leu Pro Phe 
        115                 120                 125             


Pro Val Lys Lys Ile Ala Lys Asp Phe Lys Leu Thr Val Leu Lys Gly 
    130                 135                 140                 


Asp Ile Asp Tyr His Lys Glu Arg Pro Val Gly Tyr Lys Ile Thr Pro 
145                 150                 155                 160 


Glu Glu Tyr Ala Tyr Ile Lys Asn Asp Ile Gln Ile Ile Ala Glu Ala 
                165                 170                 175     


Leu Leu Ile Gln Phe Lys Gln Gly Leu Asp Arg Met Thr Ala Gly Ser 
            180                 185                 190         


Asp Ser Leu Lys Gly Phe Lys Asp Ile Ile Thr Thr Lys Lys Phe Lys 
        195                 200                 205             


Lys Val Phe Pro Thr Leu Ser Leu Gly Leu Asp Lys Glu Val Arg Tyr 
    210                 215                 220                 


Ala Tyr Arg Gly Gly Phe Thr Trp Leu Asn Asp Arg Phe Lys Glu Lys 
225                 230                 235                 240 


Glu Ile Gly Glu Gly Met Val Phe Asp Val Asn Ser Leu Tyr Pro Ala 
                245                 250                 255     


Gln Met Tyr Ser Arg Leu Leu Pro Tyr Gly Glu Pro Ile Val Phe Glu 
            260                 265                 270         


Gly Lys Tyr Val Trp Asp Glu Asp Tyr Pro Leu His Ile Gln His Ile 
        275                 280                 285             


Arg Cys Glu Phe Glu Leu Lys Glu Gly Tyr Ile Pro Thr Ile Gln Ile 
    290                 295                 300                 


Lys Arg Ser Arg Phe Tyr Lys Gly Asn Glu Tyr Leu Lys Ser Ser Gly 
305                 310                 315                 320 


Gly Glu Ile Ala Asp Leu Trp Leu Ser Asn Val Asp Leu Glu Leu Met 
                325                 330                 335     


Lys Glu His Tyr Asp Leu Tyr Asn Val Glu Tyr Ile Ser Gly Leu Lys 
            340                 345                 350         


Phe Lys Ala Thr Thr Gly Leu Phe Lys Asp Phe Ile Asp Lys Trp Thr 
        355                 360                 365             


Tyr Ile Lys Thr Thr Ser Glu Gly Ala Ile Lys Gln Leu Ala Lys Leu 
    370                 375                 380                 


Met Leu Asn Ser Leu Tyr Gly Lys Phe Ala Ser Asn Pro Asp Val Thr 
385                 390                 395                 400 


Gly Lys Val Pro Tyr Leu Lys Glu Asn Gly Ala Leu Gly Phe Arg Leu 
                405                 410                 415     


Gly Glu Glu Glu Thr Lys Asp Pro Val Tyr Thr Pro Met Gly Val Phe 
            420                 425                 430         


Ile Thr Ala Trp Ala Arg Tyr Thr Thr Ile Thr Ala Ala Gln Ala Cys 
        435                 440                 445             


Tyr Asp Arg Ile Ile Tyr Cys Asp Thr Asp Ser Ile His Leu Thr Gly 
    450                 455                 460                 


Thr Glu Ile Pro Asp Val Ile Lys Asp Ile Val Asp Pro Lys Lys Leu 
465                 470                 475                 480 


Gly Tyr Trp Ala His Glu Ser Thr Phe Lys Arg Ala Lys Tyr Leu Arg 
                485                 490                 495     


Gln Lys Thr Tyr Ile Gln Asp Ile Tyr Met Lys Glu Val Asp Gly Lys 
            500                 505                 510         


Leu Val Glu Gly Ser Pro Asp Asp Tyr Thr Asp Ile Lys Phe Ser Val 
        515                 520                 525             


Lys Cys Ala Gly Met Thr Asp Lys Ile Lys Lys Glu Val Thr Phe Glu 
    530                 535                 540                 


Asn Phe Lys Val Gly Phe Ser Arg Lys Met Lys Pro Lys Pro Val Gln 
545                 550                 555                 560 


Val Pro Gly Gly Val Val Leu Val Asp Asp Thr Phe Thr Ile Lys Ser 
                565                 570                 575     


Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser 
            580                 585                 590         


Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
        595                 600                 605             


<210>  6
<211>  970
<212>  PRT
<213>  Citromicrobium bathyomarinum

<400>  6

Met Leu Ser Val Ala Asn Val Arg Ser Pro Ser Ala Ala Ala Ser Tyr 
1               5                   10                  15      


Phe Ala Ser Asp Asn Tyr Tyr Ala Ser Ala Asp Ala Asp Arg Ser Gly 
            20                  25                  30          


Gln Trp Ile Gly Asp Gly Ala Lys Arg Leu Gly Leu Glu Gly Lys Val 
        35                  40                  45              


Glu Ala Arg Ala Phe Asp Ala Leu Leu Arg Gly Glu Leu Pro Asp Gly 
    50                  55                  60                  


Ser Ser Val Gly Asn Pro Gly Gln Ala His Arg Pro Gly Thr Asp Leu 
65                  70                  75                  80  


Thr Phe Ser Val Pro Lys Ser Trp Ser Leu Leu Ala Leu Val Gly Lys 
                85                  90                  95      


Asp Glu Arg Ile Ile Ala Ala Tyr Arg Glu Ala Val Val Glu Ala Leu 
            100                 105                 110         


His Trp Ala Glu Lys Asn Ala Ala Glu Thr Arg Val Val Glu Lys Gly 
        115                 120                 125             


Met Val Val Thr Gln Ala Thr Gly Asn Leu Ala Ile Gly Leu Phe Gln 
    130                 135                 140                 


His Asp Thr Asn Arg Asn Gln Glu Pro Asn Leu His Phe His Ala Val 
145                 150                 155                 160 


Ile Ala Asn Val Thr Gln Gly Lys Asp Gly Lys Trp Arg Thr Leu Lys 
                165                 170                 175     


Asn Asp Arg Leu Trp Gln Leu Asn Thr Thr Leu Asn Ser Ile Ala Met 
            180                 185                 190         


Ala Arg Phe Arg Val Ala Val Glu Lys Leu Gly Tyr Glu Pro Gly Pro 
        195                 200                 205             


Val Leu Lys His Gly Asn Phe Glu Ala Arg Gly Ile Ser Arg Glu Gln 
    210                 215                 220                 


Val Met Ala Phe Ser Thr Arg Arg Lys Glu Val Leu Glu Ala Arg Arg 
225                 230                 235                 240 


Gly Pro Gly Leu Asp Ala Gly Arg Ile Ala Ala Leu Asp Thr Arg Ala 
                245                 250                 255     


Ser Lys Glu Gly Ile Glu Asp Arg Ala Thr Leu Ser Lys Gln Trp Ser 
            260                 265                 270         


Glu Ala Ala Gln Ser Ile Gly Leu Asp Leu Lys Pro Leu Val Asp Arg 
        275                 280                 285             


Ala Arg Thr Lys Ala Leu Gly Gln Gly Met Glu Ala Thr Arg Ile Gly 
    290                 295                 300                 


Ser Leu Val Glu Arg Gly Arg Ala Trp Leu Ser Arg Phe Ala Ala His 
305                 310                 315                 320 


Val Arg Gly Asp Pro Ala Asp Pro Leu Val Pro Pro Ser Val Leu Lys 
                325                 330                 335     


Gln Asp Arg Gln Thr Ile Ala Ala Ala Gln Ala Val Ala Ser Ala Val 
            340                 345                 350         


Arg His Leu Ser Gln Arg Glu Ala Ala Phe Glu Arg Thr Ala Leu Tyr 
        355                 360                 365             


Lys Ala Ala Leu Asp Phe Gly Leu Pro Thr Thr Ile Ala Asp Val Glu 
    370                 375                 380                 


Lys Arg Thr Arg Ala Leu Val Arg Ser Gly Asp Leu Ile Ala Gly Lys 
385                 390                 395                 400 


Gly Glu His Lys Gly Trp Leu Ala Ser Arg Asp Ala Val Val Thr Glu 
                405                 410                 415     


Gln Arg Ile Leu Ser Glu Val Ala Ala Gly Lys Gly Asp Ser Ser Pro 
            420                 425                 430         


Ala Ile Thr Pro Gln Lys Ala Ala Ala Ser Val Gln Ala Ala Ala Leu 
        435                 440                 445             


Thr Gly Gln Gly Phe Arg Leu Asn Glu Gly Gln Leu Ala Ala Ala Arg 
    450                 455                 460                 


Leu Ile Leu Ile Ser Lys Asp Arg Thr Ile Ala Val Gln Gly Ile Ala 
465                 470                 475                 480 


Gly Ala Gly Lys Ser Ser Val Leu Lys Pro Val Ala Glu Val Leu Arg 
                485                 490                 495     


Asp Glu Gly His Pro Val Ile Gly Leu Ala Ile Gln Asn Thr Leu Val 
            500                 505                 510         


Gln Met Leu Glu Arg Asp Thr Gly Ile Gly Ser Gln Thr Leu Ala Arg 
        515                 520                 525             


Phe Leu Gly Gly Trp Asn Lys Leu Leu Asp Asp Pro Gly Asn Val Ala 
    530                 535                 540                 


Leu Arg Ala Glu Ala Gln Ala Ser Leu Lys Asp His Val Leu Val Leu 
545                 550                 555                 560 


Asp Glu Ala Ser Met Val Ser Asn Glu Asp Lys Glu Lys Leu Val Arg 
                565                 570                 575     


Leu Ala Asn Leu Ala Gly Val His Arg Leu Val Leu Ile Gly Asp Arg 
            580                 585                 590         


Lys Gln Leu Gly Ala Val Asp Ala Gly Lys Pro Phe Ala Leu Leu Gln 
        595                 600                 605             


Arg Ala Gly Ile Ala Arg Ala Glu Met Ala Thr Asn Leu Arg Ala Arg 
    610                 615                 620                 


Asp Pro Val Val Arg Glu Ala Gln Ala Ala Ala Gln Ala Gly Asp Val 
625                 630                 635                 640 


Arg Lys Ala Leu Arg His Leu Lys Ser His Thr Val Glu Ala Arg Gly 
                645                 650                 655     


Asp Gly Ala Gln Val Ala Ala Glu Thr Trp Leu Ala Leu Asp Lys Glu 
            660                 665                 670         


Thr Arg Ala Arg Thr Ser Ile Tyr Ala Ser Gly Arg Ala Ile Arg Ser 
        675                 680                 685             


Ala Val Asn Ala Ala Val Gln Gln Gly Leu Leu Ala Ser Arg Glu Ile 
    690                 695                 700                 


Gly Pro Ala Lys Met Lys Leu Glu Val Leu Asp Arg Val Asn Thr Thr 
705                 710                 715                 720 


Arg Glu Glu Leu Arg His Leu Pro Ala Tyr Arg Ala Gly Arg Val Leu 
                725                 730                 735     


Glu Val Ser Arg Lys Gln Gln Ala Leu Gly Leu Phe Ile Gly Glu Tyr 
            740                 745                 750         


Arg Val Ile Gly Gln Asp Arg Lys Gly Lys Leu Val Glu Val Glu Asp 
        755                 760                 765             


Lys Arg Gly Lys Arg Phe Arg Phe Asp Pro Ala Arg Ile Arg Ala Gly 
    770                 775                 780                 


Lys Gly Asp Asp Asn Leu Thr Leu Leu Glu Pro Arg Lys Leu Glu Ile 
785                 790                 795                 800 


His Glu Gly Asp Arg Ile Arg Trp Thr Arg Asn Asp His Arg Arg Gly 
                805                 810                 815     


Leu Phe Asn Ala Asp Gln Ala Arg Val Val Glu Ile Ala Asn Gly Lys 
            820                 825                 830         


Val Thr Phe Glu Thr Ser Lys Gly Asp Leu Val Glu Leu Lys Lys Asp 
        835                 840                 845             


Asp Pro Met Leu Lys Arg Ile Asp Leu Ala Tyr Ala Leu Asn Val His 
    850                 855                 860                 


Met Ala Gln Gly Leu Thr Ser Asp Arg Gly Ile Ala Val Met Asp Ser 
865                 870                 875                 880 


Arg Glu Arg Asn Leu Ser Asn Gln Lys Thr Phe Leu Val Thr Val Thr 
                885                 890                 895     


Arg Leu Arg Asp His Leu Thr Leu Val Val Asp Ser Ala Asp Lys Leu 
            900                 905                 910         


Gly Ala Ala Val Ala Arg Asn Lys Gly Glu Lys Ala Ser Ala Ile Glu 
        915                 920                 925             


Val Thr Gly Ser Val Lys Pro Thr Ala Thr Lys Gly Ser Gly Val Asp 
    930                 935                 940                 


Gln Pro Lys Ser Val Glu Ala Asn Lys Ala Glu Lys Glu Leu Thr Arg 
945                 950                 955                 960 


Ser Lys Ser Lys Thr Leu Asp Phe Gly Ile 
                965                 970 


<210>  7
<211>  760
<212>  PRT
<213>  Methanococcoides burtonii

<400>  7

Met Met Ile Arg Glu Leu Asp Ile Pro Arg Asp Ile Ile Gly Phe Tyr 
1               5                   10                  15      


Glu Asp Ser Gly Ile Lys Glu Leu Tyr Pro Pro Gln Ala Glu Ala Ile 
            20                  25                  30          


Glu Met Gly Leu Leu Glu Lys Lys Asn Leu Leu Ala Ala Ile Pro Thr 
        35                  40                  45              


Ala Ser Gly Lys Thr Leu Leu Ala Glu Leu Ala Met Ile Lys Ala Ile 
    50                  55                  60                  


Arg Glu Gly Gly Lys Ala Leu Tyr Ile Val Pro Leu Arg Ala Leu Ala 
65                  70                  75                  80  


Ser Glu Lys Phe Glu Arg Phe Lys Glu Leu Ala Pro Phe Gly Ile Lys 
                85                  90                  95      


Val Gly Ile Ser Thr Gly Asp Leu Asp Ser Arg Ala Asp Trp Leu Gly 
            100                 105                 110         


Val Asn Asp Ile Ile Val Ala Thr Ser Glu Lys Thr Asp Ser Leu Leu 
        115                 120                 125             


Arg Asn Gly Thr Ser Trp Met Asp Glu Ile Thr Thr Val Val Val Asp 
    130                 135                 140                 


Glu Ile His Leu Leu Asp Ser Lys Asn Arg Gly Pro Thr Leu Glu Val 
145                 150                 155                 160 


Thr Ile Thr Lys Leu Met Arg Leu Asn Pro Asp Val Gln Val Val Ala 
                165                 170                 175     


Leu Ser Ala Thr Val Gly Asn Ala Arg Glu Met Ala Asp Trp Leu Gly 
            180                 185                 190         


Ala Ala Leu Val Leu Ser Glu Trp Arg Pro Thr Asp Leu His Glu Gly 
        195                 200                 205             


Val Leu Phe Gly Asp Ala Ile Asn Phe Pro Gly Ser Gln Lys Lys Ile 
    210                 215                 220                 


Asp Arg Leu Glu Lys Asp Asp Ala Val Asn Leu Val Leu Asp Thr Ile 
225                 230                 235                 240 


Lys Ala Glu Gly Gln Cys Leu Val Phe Glu Ser Ser Arg Arg Asn Cys 
                245                 250                 255     


Ala Gly Phe Ala Lys Thr Ala Ser Ser Lys Val Ala Lys Ile Leu Asp 
            260                 265                 270         


Asn Asp Ile Met Ile Lys Leu Ala Gly Ile Ala Glu Glu Val Glu Ser 
        275                 280                 285             


Thr Gly Glu Thr Asp Thr Ala Ile Val Leu Ala Asn Cys Ile Arg Lys 
    290                 295                 300                 


Gly Val Ala Phe His His Ala Gly Leu Asn Ser Asn His Arg Lys Leu 
305                 310                 315                 320 


Val Glu Asn Gly Phe Arg Gln Asn Leu Ile Lys Val Ile Ser Ser Thr 
                325                 330                 335     


Pro Thr Leu Ala Ala Gly Leu Asn Leu Pro Ala Arg Arg Val Ile Ile 
            340                 345                 350         


Arg Ser Tyr Arg Arg Phe Asp Ser Asn Phe Gly Met Gln Pro Ile Pro 
        355                 360                 365             


Val Leu Glu Tyr Lys Gln Met Ala Gly Arg Ala Gly Arg Pro His Leu 
    370                 375                 380                 


Asp Pro Tyr Gly Glu Ser Val Leu Leu Ala Lys Thr Tyr Asp Glu Phe 
385                 390                 395                 400 


Ala Gln Leu Met Glu Asn Tyr Val Glu Ala Asp Ala Glu Asp Ile Trp 
                405                 410                 415     


Ser Lys Leu Gly Thr Glu Asn Ala Leu Arg Thr His Val Leu Ser Thr 
            420                 425                 430         


Ile Val Asn Gly Phe Ala Ser Thr Arg Gln Glu Leu Phe Asp Phe Phe 
        435                 440                 445             


Gly Ala Thr Phe Phe Ala Tyr Gln Gln Asp Lys Trp Met Leu Glu Glu 
    450                 455                 460                 


Val Ile Asn Asp Cys Leu Glu Phe Leu Ile Asp Lys Ala Met Val Ser 
465                 470                 475                 480 


Glu Thr Glu Asp Ile Glu Asp Ala Ser Lys Leu Phe Leu Arg Gly Thr 
                485                 490                 495     


Arg Leu Gly Ser Leu Val Ser Met Leu Tyr Ile Asp Pro Leu Ser Gly 
            500                 505                 510         


Ser Lys Ile Val Asp Gly Phe Lys Asp Ile Gly Lys Ser Thr Gly Gly 
        515                 520                 525             


Asn Met Gly Ser Leu Glu Asp Asp Lys Gly Asp Asp Ile Thr Val Thr 
    530                 535                 540                 


Asp Met Thr Leu Leu His Leu Val Cys Ser Thr Pro Asp Met Arg Gln 
545                 550                 555                 560 


Leu Tyr Leu Arg Asn Thr Asp Tyr Thr Ile Val Asn Glu Tyr Ile Val 
                565                 570                 575     


Ala His Ser Asp Glu Phe His Glu Ile Pro Asp Lys Leu Lys Glu Thr 
            580                 585                 590         


Asp Tyr Glu Trp Phe Met Gly Glu Val Lys Thr Ala Met Leu Leu Glu 
        595                 600                 605             


Glu Trp Val Thr Glu Val Ser Ala Glu Asp Ile Thr Arg His Phe Asn 
    610                 615                 620                 


Val Gly Glu Gly Asp Ile His Ala Leu Ala Asp Thr Ser Glu Trp Leu 
625                 630                 635                 640 


Met His Ala Ala Ala Lys Leu Ala Glu Leu Leu Gly Val Glu Tyr Ser 
                645                 650                 655     


Ser His Ala Tyr Ser Leu Glu Lys Arg Ile Arg Tyr Gly Ser Gly Leu 
            660                 665                 670         


Asp Leu Met Glu Leu Val Gly Ile Arg Gly Val Gly Arg Val Arg Ala 
        675                 680                 685             


Arg Lys Leu Tyr Asn Ala Gly Phe Val Ser Val Ala Lys Leu Lys Gly 
    690                 695                 700                 


Ala Asp Ile Ser Val Leu Ser Lys Leu Val Gly Pro Lys Val Ala Tyr 
705                 710                 715                 720 


Asn Ile Leu Ser Gly Ile Gly Val Arg Val Asn Asp Lys His Phe Asn 
                725                 730                 735     


Ser Ala Pro Ile Ser Ser Asn Thr Leu Asp Thr Leu Leu Asp Lys Asn 
            740                 745                 750         


Gln Lys Thr Phe Asn Asp Phe Gln 
        755                 760 


<210>  8
<211>  439
<212>  PRT
<213>  Enterobacteria phage T4

<400>  8

Met Thr Phe Asp Asp Leu Thr Glu Gly Gln Lys Asn Ala Phe Asn Ile 
1               5                   10                  15      


Val Met Lys Ala Ile Lys Glu Lys Lys His His Val Thr Ile Asn Gly 
            20                  25                  30          


Pro Ala Gly Thr Gly Lys Thr Thr Leu Thr Lys Phe Ile Ile Glu Ala 
        35                  40                  45              


Leu Ile Ser Thr Gly Glu Thr Gly Ile Ile Leu Ala Ala Pro Thr His 
    50                  55                  60                  


Ala Ala Lys Lys Ile Leu Ser Lys Leu Ser Gly Lys Glu Ala Ser Thr 
65                  70                  75                  80  


Ile His Ser Ile Leu Lys Ile Asn Pro Val Thr Tyr Glu Glu Asn Val 
                85                  90                  95      


Leu Phe Glu Gln Lys Glu Val Pro Asp Leu Ala Lys Cys Arg Val Leu 
            100                 105                 110         


Ile Cys Asp Glu Val Ser Met Tyr Asp Arg Lys Leu Phe Lys Ile Leu 
        115                 120                 125             


Leu Ser Thr Ile Pro Pro Trp Cys Thr Ile Ile Gly Ile Gly Asp Asn 
    130                 135                 140                 


Lys Gln Ile Arg Pro Val Asp Pro Gly Glu Asn Thr Ala Tyr Ile Ser 
145                 150                 155                 160 


Pro Phe Phe Thr His Lys Asp Phe Tyr Gln Cys Glu Leu Thr Glu Val 
                165                 170                 175     


Lys Arg Ser Asn Ala Pro Ile Ile Asp Val Ala Thr Asp Val Arg Asn 
            180                 185                 190         


Gly Lys Trp Ile Tyr Asp Lys Val Val Asp Gly His Gly Val Arg Gly 
        195                 200                 205             


Phe Thr Gly Asp Thr Ala Leu Arg Asp Phe Met Val Asn Tyr Phe Ser 
    210                 215                 220                 


Ile Val Lys Ser Leu Asp Asp Leu Phe Glu Asn Arg Val Met Ala Phe 
225                 230                 235                 240 


Thr Asn Lys Ser Val Asp Lys Leu Asn Ser Ile Ile Arg Lys Lys Ile 
                245                 250                 255     


Phe Glu Thr Asp Lys Asp Phe Ile Val Gly Glu Ile Ile Val Met Gln 
            260                 265                 270         


Glu Pro Leu Phe Lys Thr Tyr Lys Ile Asp Gly Lys Pro Val Ser Glu 
        275                 280                 285             


Ile Ile Phe Asn Asn Gly Gln Leu Val Arg Ile Ile Glu Ala Glu Tyr 
    290                 295                 300                 


Thr Ser Thr Phe Val Lys Ala Arg Gly Val Pro Gly Glu Tyr Leu Ile 
305                 310                 315                 320 


Arg His Trp Asp Leu Thr Val Glu Thr Tyr Gly Asp Asp Glu Tyr Tyr 
                325                 330                 335     


Arg Glu Lys Ile Lys Ile Ile Ser Ser Asp Glu Glu Leu Tyr Lys Phe 
            340                 345                 350         


Asn Leu Phe Leu Gly Lys Thr Ala Glu Thr Tyr Lys Asn Trp Asn Lys 
        355                 360                 365             


Gly Gly Lys Ala Pro Trp Ser Asp Phe Trp Asp Ala Lys Ser Gln Phe 
    370                 375                 380                 


Ser Lys Val Lys Ala Leu Pro Ala Ser Thr Phe His Lys Ala Gln Gly 
385                 390                 395                 400 


Met Ser Val Asp Arg Ala Phe Ile Tyr Thr Pro Cys Ile His Tyr Ala 
                405                 410                 415     


Asp Val Glu Leu Ala Gln Gln Leu Leu Tyr Val Gly Val Thr Arg Gly 
            420                 425                 430         


Arg Tyr Asp Val Phe Tyr Val 
        435                 


<210>  9

<400>  9
000

<210>  10
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LukF subunit of gamma-hemolysin (Del 1-15)

<400>  10

Ala Glu Gly Lys Ile Thr Pro Val Ser Val Lys Lys Val Asp Asp Lys 
1               5                   10                  15      


Val Thr Leu Tyr Lys Thr Thr Ala Thr Ala Asp Ser Asp Lys Phe Lys 
            20                  25                  30          


Ile Ser Gln Ile Leu Thr Phe Asn Phe Ile Lys Asp Lys Ser Tyr Asp 
        35                  40                  45              


Lys Asp Thr Leu Val Leu Lys Ala Thr Gly Asn Ile Asn Ser Gly Phe 
    50                  55                  60                  


Val Lys Pro Asn Pro Asn Asp Tyr Asp Phe Ser Lys Leu Tyr Trp Gly 
65                  70                  75                  80  


Ala Lys Tyr Asn Val Ser Ile Ser Ser Gln Ser Asn Asp Ser Val Asn 
                85                  90                  95      


Val Val Asp Tyr Ala Pro Lys Asn Gln Asn Glu Glu Phe Gln Val Gln 
            100                 105                 110         


Asn Thr Leu Gly Tyr Thr Phe Gly Gly Asp Ile Ser Ile Ser Asn Gly 
        115                 120                 125             


Leu Ser Gly Gly Leu Asn Gly Asn Thr Ala Phe Ser Glu Thr Ile Asn 
    130                 135                 140                 


Tyr Lys Gln Glu Ser Tyr Arg Thr Thr Leu Ser Arg Asn Thr Asn Tyr 
145                 150                 155                 160 


Lys Asn Val Gly Trp Gly Val Glu Ala His Lys Ile Met Asn Asn Gly 
                165                 170                 175     


Trp Gly Pro Tyr Gly Arg Asp Ser Phe His Pro Thr Tyr Gly Asn Glu 
            180                 185                 190         


Leu Phe Leu Ala Gly Arg Gln Ser Ser Ala Tyr Ala Gly Gln Asn Phe 
        195                 200                 205             


Ile Ala Gln His Gln Met Pro Leu Leu Ser Arg Ser Asn Phe Asn Pro 
    210                 215                 220                 


Glu Phe Leu Ser Val Leu Ser His Arg Gln Asp Gly Ala Lys Lys Ser 
225                 230                 235                 240 


Lys Ile Thr Val Thr Tyr Gln Arg Glu Met Asp Leu Tyr Gln Ile Arg 
                245                 250                 255     


Trp Asn Gly Phe Tyr Trp Ala Gly Ala Asn Tyr Lys Asn Phe Lys Thr 
            260                 265                 270         


Arg Thr Phe Lys Ser Thr Tyr Glu Ile Asp Trp Glu Asn His Lys Val 
        275                 280                 285             


Lys Leu Leu Asp Thr Lys Glu Thr Glu Asn Asn Lys 
    290                 295                 300 


<210>  11
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hlg2 subunit of gamma-hemolysin (Del 1-10)

<400>  11

Glu Asn Lys Ile Glu Asp Ile Gly Gln Gly Ala Glu Ile Ile Lys Arg 
1               5                   10                  15      


Thr Gln Asp Ile Thr Ser Lys Arg Leu Ala Ile Thr Gln Asn Ile Gln 
            20                  25                  30          


Phe Asp Phe Val Lys Asp Lys Lys Tyr Asn Lys Asp Ala Leu Val Val 
        35                  40                  45              


Lys Met Gln Gly Phe Ile Ser Ser Arg Thr Thr Tyr Ser Asp Leu Lys 
    50                  55                  60                  


Lys Tyr Pro Tyr Ile Lys Arg Met Ile Trp Pro Phe Gln Tyr Asn Ile 
65                  70                  75                  80  


Ser Leu Lys Thr Lys Asp Ser Asn Val Asp Leu Ile Asn Tyr Leu Pro 
                85                  90                  95      


Lys Asn Lys Ile Asp Ser Ala Asp Val Ser Gln Lys Leu Gly Tyr Asn 
            100                 105                 110         


Ile Gly Gly Asn Phe Gln Ser Ala Pro Ser Ile Gly Gly Ser Gly Ser 
        115                 120                 125             


Phe Asn Tyr Ser Lys Thr Ile Ser Tyr Asn Gln Lys Asn Tyr Val Thr 
    130                 135                 140                 


Glu Val Glu Ser Gln Asn Ser Lys Gly Val Lys Trp Gly Val Lys Ala 
145                 150                 155                 160 


Asn Ser Phe Val Thr Pro Asn Gly Gln Val Ser Ala Tyr Asp Gln Tyr 
                165                 170                 175     


Leu Phe Ala Gln Asp Pro Thr Gly Pro Ala Ala Arg Asp Tyr Phe Val 
            180                 185                 190         


Pro Asp Asn Gln Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ser 
        195                 200                 205             


Phe Ile Thr Thr Leu Ser His Glu Arg Gly Lys Gly Asp Lys Ser Glu 
    210                 215                 220                 


Phe Glu Ile Thr Tyr Gly Arg Asn Met Asp Ala Thr Tyr Ala Tyr Val 
225                 230                 235                 240 


Thr Arg His Arg Leu Ala Val Asp Arg Lys His Asp Ala Phe Lys Asn 
                245                 250                 255     


Arg Asn Val Thr Val Lys Tyr Glu Val Asn Trp Lys Thr His Glu Val 
            260                 265                 270         


Lys Ile Lys Ser Ile Thr Pro Lys 
        275                 280 


<210>  12
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of a monomer of 
       lysenin-(E84Q/E85S/E92Q/E94D/E97S/T106K/D126G)

<400>  12

Met Ser Ala Lys Ala Ala Glu Gly Tyr Glu Gln Ile Glu Val Asp Val 
1               5                   10                  15      


Val Ala Val Trp Lys Glu Gly Tyr Val Tyr Glu Asn Arg Gly Ser Thr 
            20                  25                  30          


Ser Val Asp Gln Lys Ile Thr Ile Thr Lys Gly Met Lys Asn Val Asn 
        35                  40                  45              


Ser Glu Thr Arg Thr Val Thr Ala Thr His Ser Ile Gly Ser Thr Ile 
    50                  55                  60                  


Ser Thr Gly Asp Ala Phe Glu Ile Gly Ser Val Glu Val Ser Tyr Ser 
65                  70                  75                  80  


His Ser His Gln Lys Ser Gln Val Ser Met Thr Gln Thr Asp Val Tyr 
                85                  90                  95      


Ser Ser Lys Val Ile Glu His Thr Ile Lys Ile Pro Pro Thr Ser Lys 
            100                 105                 110         


Phe Thr Arg Trp Gln Leu Asn Ala Asp Val Gly Gly Ala Gly Ile Glu 
        115                 120                 125             


Tyr Met Tyr Leu Ile Asp Glu Val Thr Pro Ile Gly Gly Thr Gln Ser 
    130                 135                 140                 


Ile Pro Gln Val Ile Thr Ser Arg Ala Lys Ile Ile Val Gly Arg Gln 
145                 150                 155                 160 


Ile Ile Leu Gly Lys Thr Glu Ile Arg Ile Lys His Ala Glu Arg Lys 
                165                 170                 175     


Glu Tyr Met Thr Val Val Ser Arg Lys Ser Trp Pro Ala Ala Thr Leu 
            180                 185                 190         


Gly His Ser Lys Leu Phe Lys Phe Val Leu Tyr Glu Asp Trp Gly Gly 
        195                 200                 205             


Phe Arg Ile Lys Thr Leu Asn Thr Met Tyr Ser Gly Tyr Glu Tyr Ala 
    210                 215                 220                 


Tyr Ser Ser Asp Gln Gly Gly Ile Tyr Phe Asp Gln Gly Thr Asp Asn 
225                 230                 235                 240 


Pro Lys Gln Arg Trp Ala Ile Asn Lys Ser Leu Pro Leu Arg His Gly 
                245                 250                 255     


Asp Val Val Thr Phe Met Asn Lys Tyr Phe Thr Arg Ser Gly Leu Cys 
            260                 265                 270         


Tyr Asp Asp Gly Pro Ala Thr Asn Val Tyr Cys Leu Asp Lys Arg Glu 
        275                 280                 285             


Asp Lys Trp Ile Leu Glu Val Val Gly 
    290                 295         


<210>  13
<211>  306
<212>  PRT
<213>  Bacillus cereus

<400>  13

Met Gln Thr Thr Ser Gln Val Val Thr Asp Ile Gly Gln Asn Ala Lys 
1               5                   10                  15      


Thr His Thr Ser Tyr Asn Thr Phe Asn Asn Glu Gln Ala Asp Asn Met 
            20                  25                  30          


Thr Met Ser Leu Lys Val Thr Phe Ile Asp Asp Pro Ser Ala Asp Lys 
        35                  40                  45              


Gln Ile Ala Val Ile Asn Thr Thr Gly Ser Phe Met Lys Ala Asn Pro 
    50                  55                  60                  


Thr Leu Ser Asp Ala Pro Val Asp Gly Tyr Pro Ile Pro Gly Ala Ser 
65                  70                  75                  80  


Val Thr Leu Arg Tyr Pro Ser Gln Tyr Asp Ile Ala Met Asn Leu Gln 
                85                  90                  95      


Asp Asn Thr Ser Arg Phe Phe His Val Ala Pro Thr Asn Ala Val Glu 
            100                 105                 110         


Glu Thr Thr Val Thr Ser Ser Val Ser Tyr Gln Leu Gly Gly Ser Ile 
        115                 120                 125             


Lys Ala Ser Val Thr Pro Ser Gly Pro Ser Gly Glu Ser Gly Ala Thr 
    130                 135                 140                 


Gly Gln Val Thr Trp Ser Asp Ser Val Ser Tyr Lys Gln Thr Ser Tyr 
145                 150                 155                 160 


Lys Thr Asn Leu Ile Asp Gln Thr Asn Lys His Val Lys Trp Asn Val 
                165                 170                 175     


Phe Phe Asn Gly Tyr Asn Asn Gln Asn Trp Gly Ile Tyr Thr Arg Asp 
            180                 185                 190         


Ser Tyr His Ala Leu Tyr Gly Asn Gln Leu Phe Met Tyr Ser Arg Thr 
        195                 200                 205             


Tyr Pro His Glu Thr Asp Ala Arg Gly Asn Leu Val Pro Met Asn Asp 
    210                 215                 220                 


Leu Pro Ala Leu Thr Asn Ser Gly Phe Ser Pro Gly Met Ile Ala Val 
225                 230                 235                 240 


Val Ile Ser Glu Lys Asp Thr Glu Gln Ser Ser Ile Gln Val Ala Tyr 
                245                 250                 255     


Thr Lys His Ala Asp Asp Tyr Thr Leu Arg Pro Gly Phe Thr Phe Gly 
            260                 265                 270         


Thr Gly Asn Trp Val Gly Asn Asn Ile Lys Asp Val Asp Gln Lys Thr 
        275                 280                 285             


Phe Asn Lys Ser Phe Val Leu Asp Trp Lys Asn Lys Lys Leu Val Glu 
    290                 295                 300                 


Lys Lys 
305     


<210>  14
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a monomer of aerolysin

<400>  14

Ala Glu Pro Val Tyr Pro Asp Gln Leu Arg Leu Phe Ser Leu Gly Gln 
1               5                   10                  15      


Gly Val Cys Gly Asp Lys Tyr Arg Pro Val Asn Arg Glu Glu Ala Gln 
            20                  25                  30          


Ser Val Lys Ser Asn Ile Val Gly Met Met Gly Gln Trp Gln Ile Ser 
        35                  40                  45              


Gly Leu Ala Asn Gly Trp Val Ile Met Gly Pro Gly Tyr Asn Gly Glu 
    50                  55                  60                  


Ile Lys Pro Gly Thr Ala Ser Asn Thr Trp Cys Tyr Pro Thr Asn Pro 
65                  70                  75                  80  


Val Thr Gly Glu Ile Pro Thr Leu Ser Ala Leu Asp Ile Pro Asp Gly 
                85                  90                  95      


Asp Glu Val Asp Val Gln Trp Arg Leu Val His Asp Ser Ala Asn Phe 
            100                 105                 110         


Ile Lys Pro Thr Ser Tyr Leu Ala His Tyr Leu Gly Tyr Ala Trp Val 
        115                 120                 125             


Gly Gly Asn His Ser Gln Tyr Val Gly Glu Asp Met Asp Val Thr Arg 
    130                 135                 140                 


Asp Gly Asp Gly Trp Val Ile Arg Gly Asn Asn Asp Gly Gly Cys Asp 
145                 150                 155                 160 


Gly Tyr Arg Cys Gly Asp Lys Thr Ala Ile Lys Val Ser Asn Phe Ala 
                165                 170                 175     


Tyr Asn Leu Asp Pro Asp Ser Phe Lys His Gly Asp Val Thr Gln Ser 
            180                 185                 190         


Asp Arg Gln Leu Val Lys Thr Val Val Gly Trp Ala Val Asn Asp Ser 
        195                 200                 205             


Asp Thr Pro Gln Ser Gly Tyr Asp Val Thr Leu Arg Tyr Asp Thr Ala 
    210                 215                 220                 


Thr Asn Trp Ser Lys Thr Asn Thr Tyr Gly Leu Ser Glu Lys Val Thr 
225                 230                 235                 240 


Thr Lys Asn Lys Phe Lys Trp Pro Leu Val Gly Glu Thr Glu Leu Ser 
                245                 250                 255     


Ile Glu Ile Ala Ala Asn Gln Ser Trp Ala Ser Gln Asn Gly Gly Ser 
            260                 265                 270         


Thr Thr Thr Ser Leu Ser Gln Ser Val Arg Pro Thr Val Pro Ala Arg 
        275                 280                 285             


Ser Lys Ile Pro Val Lys Ile Glu Leu Tyr Lys Ala Asp Ile Ser Tyr 
    290                 295                 300                 


Pro Tyr Glu Phe Lys Ala Asp Val Ser Tyr Asp Leu Thr Leu Ser Gly 
305                 310                 315                 320 


Phe Leu Arg Trp Gly Gly Asn Ala Trp Tyr Thr His Pro Asp Asn Arg 
                325                 330                 335     


Pro Asn Trp Asn His Thr Phe Val Ile Gly Pro Tyr Lys Asp Lys Ala 
            340                 345                 350         


Ser Ser Ile Arg Tyr Gln Trp Asp Lys Arg Tyr Ile Pro Gly Glu Val 
        355                 360                 365             


Lys Trp Trp Asp Trp Asn Trp Thr Ile Gln Gln Asn Gly Leu Ser Thr 
    370                 375                 380                 


Met Gln Asn Asn Leu Ala Arg Val Leu Arg Pro Val Arg Ala Gly Ile 
385                 390                 395                 400 


Thr Gly Asp Phe Ser Ala Glu Ser Gln Phe Ala Gly Asn Ile Glu Ile 
                405                 410                 415     


Gly Ala Pro Val Pro Leu Ala Ala 
            420                 


<210>  15
<211>  292
<212>  PRT
<213>  Clostridium perfringens

<400>  15

Ser Glu Leu Asn Asp Ile Asn Lys Ile Glu Leu Lys Asn Leu Ser Gly 
1               5                   10                  15      


Glu Ile Ile Lys Glu Asn Gly Lys Glu Ala Ile Lys Tyr Thr Ser Ser 
            20                  25                  30          


Asp Thr Ala Ser His Lys Gly Trp Lys Ala Thr Leu Ser Gly Thr Phe 
        35                  40                  45              


Ile Glu Asp Pro His Ser Asp Lys Lys Thr Ala Leu Leu Asn Leu Glu 
    50                  55                  60                  


Gly Phe Ile Pro Ser Asp Lys Gln Ile Phe Gly Ser Lys Tyr Tyr Gly 
65                  70                  75                  80  


Lys Met Lys Trp Pro Glu Thr Tyr Arg Ile Asn Val Lys Ser Ala Asp 
                85                  90                  95      


Val Asn Asn Asn Ile Lys Ile Ala Asn Ser Ile Pro Lys Asn Thr Ile 
            100                 105                 110         


Asp Lys Lys Asp Val Ser Asn Ser Ile Gly Tyr Ser Ile Gly Gly Asn 
        115                 120                 125             


Ile Ser Val Glu Gly Lys Thr Ala Gly Ala Gly Ile Asn Ala Ser Tyr 
    130                 135                 140                 


Asn Val Gln Asn Thr Ile Ser Tyr Glu Gln Pro Asp Phe Arg Thr Ile 
145                 150                 155                 160 


Gln Arg Lys Asp Asp Ala Asn Leu Ala Ser Trp Asp Ile Lys Phe Val 
                165                 170                 175     


Glu Thr Lys Asp Gly Tyr Asn Ile Asp Ser Tyr His Ala Ile Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Ser Arg Leu Tyr Asn Asn Gly Asp Lys Asn 
        195                 200                 205             


Phe Thr Asp Asp Arg Asp Leu Ser Thr Leu Ile Ser Gly Gly Phe Ser 
    210                 215                 220                 


Pro Asn Met Ala Leu Ala Leu Thr Ala Pro Lys Asn Ala Lys Glu Ser 
225                 230                 235                 240 


Val Ile Ile Val Glu Tyr Gln Arg Phe Asp Asn Asp Tyr Ile Leu Asn 
                245                 250                 255     


Trp Glu Thr Thr Gln Trp Arg Gly Thr Asn Lys Leu Ser Ser Thr Ser 
            260                 265                 270         


Glu Tyr Asn Glu Phe Met Phe Lys Ile Asn Trp Gln Asp His Lys Ile 
        275                 280                 285             


Glu Tyr Tyr Leu 
    290         


<210>  16
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a monomer of alpha-hemolysin

<400>  16

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  17
<211>  716
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a monomer of Vibrio cholera cytolysin 
       (HlyA) / VCC

<400>  17

Asn Ile Asn Glu Pro Ser Gly Glu Ala Ala Asp Ile Ile Ser Gln Val 
1               5                   10                  15      


Ala Asp Ser His Ala Ile Lys Tyr Tyr Asn Ala Ala Asp Trp Gln Ala 
            20                  25                  30          


Glu Asp Asn Ala Leu Pro Ser Leu Ala Glu Leu Arg Asp Leu Val Ile 
        35                  40                  45              


Asn Gln Gln Lys Arg Val Leu Val Asp Phe Ser Gln Ile Ser Asp Ala 
    50                  55                  60                  


Glu Gly Gln Ala Glu Met Gln Ala Gln Phe Arg Lys Ala Tyr Gly Val 
65                  70                  75                  80  


Gly Phe Ala Asn Gln Phe Ile Val Ile Thr Glu His Lys Gly Glu Leu 
                85                  90                  95      


Leu Phe Thr Pro Phe Asp Gln Ala Glu Glu Val Asp Pro Gln Leu Leu 
            100                 105                 110         


Glu Ala Pro Arg Thr Ala Arg Leu Leu Ala Arg Ser Gly Phe Ala Ser 
        115                 120                 125             


Pro Ala Pro Ala Asn Ser Glu Thr Asn Thr Leu Pro His Val Ala Phe 
    130                 135                 140                 


Tyr Ile Ser Val Asn Arg Ala Ile Ser Asp Glu Glu Cys Thr Phe Asn 
145                 150                 155                 160 


Asn Ser Trp Leu Trp Lys Asn Glu Lys Gly Ser Arg Pro Phe Cys Lys 
                165                 170                 175     


Asp Ala Asn Ile Ser Leu Ile Tyr Arg Val Asn Leu Glu Arg Ser Leu 
            180                 185                 190         


Gln Tyr Gly Ile Val Gly Ser Ala Thr Pro Asp Ala Lys Ile Val Arg 
        195                 200                 205             


Ile Ser Leu Asp Asp Asp Ser Thr Gly Ala Gly Ile His Leu Asn Asp 
    210                 215                 220                 


Gln Leu Gly Tyr Arg Gln Phe Gly Ala Ser Tyr Thr Thr Leu Asp Ala 
225                 230                 235                 240 


Tyr Phe Arg Glu Trp Ser Thr Asp Ala Ile Ala Gln Asp Tyr Arg Phe 
                245                 250                 255     


Val Phe Asn Ala Ser Asn Asn Lys Ala Gln Ile Leu Lys Thr Phe Pro 
            260                 265                 270         


Val Asp Asn Ile Asn Glu Lys Phe Glu Arg Lys Glu Val Ser Gly Phe 
        275                 280                 285             


Glu Leu Gly Val Thr Gly Gly Val Glu Val Ser Gly Asp Gly Pro Lys 
    290                 295                 300                 


Ala Lys Leu Glu Ala Arg Ala Ser Tyr Thr Gln Ser Arg Trp Leu Thr 
305                 310                 315                 320 


Tyr Asn Thr Gln Asp Tyr Arg Ile Glu Arg Asn Ala Lys Asn Ala Gln 
                325                 330                 335     


Ala Val Ser Phe Thr Trp Asn Arg Gln Gln Tyr Ala Thr Ala Glu Ser 
            340                 345                 350         


Leu Leu Asn Arg Ser Thr Asp Ala Leu Trp Val Asn Thr Tyr Pro Val 
        355                 360                 365             


Asp Val Asn Arg Ile Ser Pro Leu Ser Tyr Ala Ser Phe Val Pro Lys 
    370                 375                 380                 


Met Asp Val Ile Tyr Lys Ala Ser Ala Thr Glu Thr Gly Ser Thr Asp 
385                 390                 395                 400 


Phe Ile Ile Asp Ser Ser Val Asn Ile Arg Pro Ile Tyr Asn Gly Ala 
                405                 410                 415     


Tyr Lys His Tyr Tyr Val Val Gly Ala His Gln Ser Tyr His Gly Phe 
            420                 425                 430         


Glu Asp Thr Pro Arg Arg Arg Ile Thr Lys Ser Ala Ser Phe Thr Val 
        435                 440                 445             


Asp Trp Asp His Pro Val Phe Thr Gly Gly Arg Pro Val Asn Leu Gln 
    450                 455                 460                 


Leu Ala Ser Phe Asn Asn Arg Cys Ile Gln Val Asp Ala Gln Gly Arg 
465                 470                 475                 480 


Leu Ala Ala Asn Thr Cys Asp Ser Gln Gln Ser Ala Gln Ser Phe Ile 
                485                 490                 495     


Tyr Asp Gln Leu Gly Arg Tyr Val Ser Ala Ser Asn Thr Lys Leu Cys 
            500                 505                 510         


Leu Asp Gly Glu Ala Leu Asp Ala Leu Gln Pro Cys Asn Gln Asn Leu 
        515                 520                 525             


Thr Gln Arg Trp Glu Trp Arg Lys Gly Thr Asp Glu Leu Thr Asn Val 
    530                 535                 540                 


Tyr Ser Gly Glu Ser Leu Gly His Asp Lys Gln Thr Gly Glu Leu Gly 
545                 550                 555                 560 


Leu Tyr Ala Ser Ser Asn Asp Ala Val Ser Leu Arg Thr Ile Thr Ala 
                565                 570                 575     


Tyr Thr Asp Val Phe Asn Ala Gln Glu Ser Ser Pro Ile Leu Gly Tyr 
            580                 585                 590         


Thr Gln Gly Lys Met Asn Gln Gln Arg Val Gly Gln Asp His Arg Leu 
        595                 600                 605             


Tyr Val Arg Ala Gly Ala Ala Ile Asp Ala Leu Gly Ser Ala Ser Asp 
    610                 615                 620                 


Leu Leu Val Gly Gly Asn Gly Gly Ser Leu Ser Ser Val Asp Leu Ser 
625                 630                 635                 640 


Gly Val Lys Ser Ile Thr Ala Thr Ser Gly Asp Phe Gln Tyr Gly Gly 
                645                 650                 655     


Gln Gln Leu Val Ala Leu Thr Phe Thr Tyr Gln Asp Gly Arg Gln Gln 
            660                 665                 670         


Thr Val Gly Ser Lys Ala Tyr Val Thr Asn Ala His Glu Asp Arg Phe 
        675                 680                 685             


Asp Leu Pro Ala Ala Ala Lys Ile Thr Gln Leu Lys Ile Trp Ser Asp 
    690                 695                 700                 


Asp Trp Leu Val Lys Gly Val Gln Phe Asp Leu Asn 
705                 710                 715     


<210>  18
<211>  735
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a monomer of Anthrax toxin protective 
       antigen (Anthrax PA).

<400>  18

Glu Val Lys Gln Glu Asn Arg Leu Leu Asn Glu Ser Glu Ser Ser Ser 
1               5                   10                  15      


Gln Gly Leu Leu Gly Tyr Tyr Phe Ser Asp Leu Asn Phe Gln Ala Pro 
            20                  25                  30          


Met Val Val Thr Ser Ser Thr Thr Gly Asp Leu Ser Ile Pro Ser Ser 
        35                  40                  45              


Glu Leu Glu Asn Ile Pro Ser Glu Asn Gln Tyr Phe Gln Ser Ala Ile 
    50                  55                  60                  


Trp Ser Gly Phe Ile Lys Val Lys Lys Ser Asp Glu Tyr Thr Phe Ala 
65                  70                  75                  80  


Thr Ser Ala Asp Asn His Val Thr Met Trp Val Asp Asp Gln Glu Val 
                85                  90                  95      


Ile Asn Lys Ala Ser Asn Ser Asn Lys Ile Arg Leu Glu Lys Gly Arg 
            100                 105                 110         


Leu Tyr Gln Ile Lys Ile Gln Tyr Gln Arg Glu Asn Pro Thr Glu Lys 
        115                 120                 125             


Gly Leu Asp Phe Lys Leu Tyr Trp Thr Asp Ser Gln Asn Lys Lys Glu 
    130                 135                 140                 


Val Ile Ser Ser Asp Asn Leu Gln Leu Pro Glu Leu Lys Gln Lys Ser 
145                 150                 155                 160 


Ser Asn Ser Arg Lys Lys Arg Ser Thr Ser Ala Gly Pro Thr Val Pro 
                165                 170                 175     


Asp Arg Asp Asn Asp Gly Ile Pro Asp Ser Leu Glu Val Glu Gly Tyr 
            180                 185                 190         


Thr Val Asp Val Lys Asn Lys Arg Thr Phe Leu Ser Pro Trp Ile Ser 
        195                 200                 205             


Asn Ile His Glu Lys Lys Gly Leu Thr Lys Tyr Lys Ser Ser Pro Glu 
    210                 215                 220                 


Lys Trp Ser Thr Ala Ser Asp Pro Tyr Ser Asp Phe Glu Lys Val Thr 
225                 230                 235                 240 


Gly Arg Ile Asp Lys Asn Val Ser Pro Glu Ala Arg His Pro Leu Val 
                245                 250                 255     


Ala Ala Tyr Pro Ile Val His Val Asp Met Glu Asn Ile Ile Leu Ser 
            260                 265                 270         


Lys Asn Glu Asp Gln Ser Thr Gln Asn Thr Asp Ser Gln Thr Arg Thr 
        275                 280                 285             


Ile Ser Lys Asn Thr Ser Thr Ser Arg Thr His Thr Ser Glu Val His 
    290                 295                 300                 


Gly Asn Ala Glu Val His Ala Ser Phe Phe Asp Ile Gly Gly Ser Val 
305                 310                 315                 320 


Ser Ala Gly Phe Ser Asn Ser Asn Ser Ser Thr Val Ala Ile Asp His 
                325                 330                 335     


Ser Leu Ser Leu Ala Gly Glu Arg Thr Trp Ala Glu Thr Met Gly Leu 
            340                 345                 350         


Asn Thr Ala Asp Thr Ala Arg Leu Asn Ala Asn Ile Arg Tyr Val Asn 
        355                 360                 365             


Thr Gly Thr Ala Pro Ile Tyr Asn Val Leu Pro Thr Thr Ser Leu Val 
    370                 375                 380                 


Leu Gly Lys Asn Gln Thr Leu Ala Thr Ile Lys Ala Lys Glu Asn Gln 
385                 390                 395                 400 


Leu Ser Gln Ile Leu Ala Pro Asn Asn Tyr Tyr Pro Ser Lys Asn Leu 
                405                 410                 415     


Ala Pro Ile Ala Leu Asn Ala Gln Asp Asp Phe Ser Ser Thr Pro Ile 
            420                 425                 430         


Thr Met Asn Tyr Asn Gln Phe Leu Glu Leu Glu Lys Thr Lys Gln Leu 
        435                 440                 445             


Arg Leu Asp Thr Asp Gln Val Tyr Gly Asn Ile Ala Thr Tyr Asn Phe 
    450                 455                 460                 


Glu Asn Gly Arg Val Arg Val Asp Thr Gly Ser Asn Trp Ser Glu Val 
465                 470                 475                 480 


Leu Pro Gln Ile Gln Glu Thr Thr Ala Arg Ile Ile Phe Asn Gly Lys 
                485                 490                 495     


Asp Leu Asn Leu Val Glu Arg Arg Ile Ala Ala Val Asn Pro Ser Asp 
            500                 505                 510         


Pro Leu Glu Thr Thr Lys Pro Asp Met Thr Leu Lys Glu Ala Leu Lys 
        515                 520                 525             


Ile Ala Phe Gly Phe Asn Glu Pro Asn Gly Asn Leu Gln Tyr Gln Gly 
    530                 535                 540                 


Lys Asp Ile Thr Glu Phe Asp Phe Asn Phe Asp Gln Gln Thr Ser Gln 
545                 550                 555                 560 


Asn Ile Lys Asn Gln Leu Ala Glu Leu Asn Ala Thr Asn Ile Tyr Thr 
                565                 570                 575     


Val Leu Asp Lys Ile Lys Leu Asn Ala Lys Met Asn Ile Leu Ile Arg 
            580                 585                 590         


Asp Lys Arg Phe His Tyr Asp Arg Asn Asn Ile Ala Val Gly Ala Asp 
        595                 600                 605             


Glu Ser Val Val Lys Glu Ala His Arg Glu Val Ile Asn Ser Ser Thr 
    610                 615                 620                 


Glu Gly Leu Leu Leu Asn Ile Asp Lys Asp Ile Arg Lys Ile Leu Ser 
625                 630                 635                 640 


Gly Tyr Ile Val Glu Ile Glu Asp Thr Glu Gly Leu Lys Glu Val Ile 
                645                 650                 655     


Asn Asp Arg Tyr Asp Met Leu Asn Ile Ser Ser Leu Arg Gln Asp Gly 
            660                 665                 670         


Lys Thr Phe Ile Asp Phe Lys Lys Tyr Asn Asp Lys Leu Pro Leu Tyr 
        675                 680                 685             


Ile Ser Asn Pro Asn Tyr Lys Val Asn Val Tyr Ala Val Thr Lys Glu 
    690                 695                 700                 


Asn Thr Ile Ile Asn Pro Ser Glu Asn Gly Asp Thr Ser Thr Asn Gly 
705                 710                 715                 720 


Ile Lys Lys Ile Leu Ile Phe Ser Lys Lys Gly Tyr Glu Ile Gly 
                725                 730                 735 


<210>  19
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a monomer of epsilon toxin

<400>  19

Lys Ala Ser Tyr Asp Asn Val Asp Thr Leu Ile Glu Lys Gly Arg Tyr 
1               5                   10                  15      


Asn Thr Lys Tyr Asn Tyr Leu Lys Arg Met Glu Lys Tyr Tyr Pro Asn 
            20                  25                  30          


Ala Met Ala Tyr Phe Asp Lys Val Thr Ile Asn Pro Gln Gly Asn Asp 
        35                  40                  45              


Phe Tyr Ile Asn Asn Pro Lys Val Glu Leu Asp Gly Glu Pro Ser Met 
    50                  55                  60                  


Asn Tyr Leu Glu Asp Val Tyr Val Gly Lys Ala Leu Leu Thr Asn Asp 
65                  70                  75                  80  


Thr Gln Gln Glu Gln Lys Leu Lys Ser Gln Ser Phe Thr Cys Lys Asn 
                85                  90                  95      


Thr Asp Thr Val Thr Ala Thr Thr Thr His Thr Val Gly Thr Ser Ile 
            100                 105                 110         


Gln Ala Thr Ala Lys Phe Thr Val Pro Phe Asn Glu Thr Gly Val Ser 
        115                 120                 125             


Leu Thr Thr Ser Tyr Ser Phe Ala Asn Thr Asn Thr Asn Thr Asn Ser 
    130                 135                 140                 


Lys Glu Ile Thr His Asn Val Pro Ser Gln Asp Ile Leu Val Pro Ala 
145                 150                 155                 160 


Asn Thr Thr Val Glu Val Ile Ala Tyr Leu Lys Lys Val Asn Val Lys 
                165                 170                 175     


Gly Asn Val Lys Leu Val Gly Gln Val Ser Gly Ser Glu Trp Gly Glu 
            180                 185                 190         


Ile Pro Ser Tyr Leu Ala Phe Pro Arg Asp Gly Tyr Lys Phe Ser Leu 
        195                 200                 205             


Ser Asp Thr Val Asn Lys Ser Asp Leu Asn Glu Asp Gly Thr Ile Asn 
    210                 215                 220                 


Ile Asn Gly Lys Gly Asn Tyr Ser Ala Val Met Gly Asp Glu Leu Ile 
225                 230                 235                 240 


Val Lys Val Arg Asn Leu Asn Thr Asn Asn Val Gln Glu Tyr Val Ile 
                245                 250                 255     


Pro Val Asp Lys Lys Glu Lys Ser Asn Asp Ser Asn Ile Val Lys Tyr 
            260                 265                 270         


Arg Ser Leu Tyr Ile Lys Ala Pro Gly Ile Lys 
        275                 280             


<210>  20
<211>  3560
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3.6 kb lambda DNA analyte

<400>  20
gccatcagat tgtgtttgtt agtcgctttt tttttttgga attttttttt tggaattttt       60

tttttgcgct aacaacctcc tgccgttttg cccgtgcata tcggtcacga acaaatctga      120

ttactaaaca cagtagcctg gatttgttct atcagtaatc gaccttattc ctaattaaat      180

agagcaaatc cccttattgg gggtaagaca tgaagatgcc agaaaaacat gacctgttgg      240

ccgccattct cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt gcaatggcgt      300

accttcgcgg cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt      360

gcgccattat cgcctagttc attcgtgacc ttctcgactt cgccggacta agtagcaatc      420

tcgcttatat aacgagcgtg tttatcggct acatcggtac tgactcgatt ggttcgctta      480

tcaaacgctt cgctgctaaa aaagccggag tagaagatgg tagaaatcaa taatcaacgt      540

aaggcgttcc tcgatatgct ggcgtggtcg gagggaactg ataacggacg tcagaaaacc      600

agaaatcatg gttatgacgt cattgtaggc ggagagctat ttactgatta ctccgatcac      660

cctcgcaaac ttgtcacgct aaacccaaaa ctcaaatcaa caggcgccgg acgctaccag      720

cttctttccc gttggtggga tgcctaccgc aagcagcttg gcctgaaaga cttctctccg      780

aaaagtcagg acgctgtggc attgcagcag attaaggagc gtggcgcttt acctatgatt      840

gatcgtggtg atatccgtca ggcaatcgac cgttgcagca atatctgggc ttcactgccg      900

ggcgctggtt atggtcagtt cgagcataag gctgacagcc tgattgcaaa attcaaagaa      960

gcgggcggaa cggtcagaga gattgatgta tgagcagagt caccgcgatt atctccgctc     1020

tggttatctg catcatcgtc tgcctgtcat gggctgttaa tcattaccgt gataacgcca     1080

ttacctacaa agcccagcgc gacaaaaatg ccagagaact gaagctggcg aacgcggcaa     1140

ttactgacat gcagatgcgt cagcgtgatg ttgctgcgct cgatgcaaaa tacacgaagg     1200

agttagctga tgctaaagct gaaaatgatg ctctgcgtga tgatgttgcc gctggtcgtc     1260

gtcggttgca catcaaagca gtctgtcagt cagtgcgtga agccaccacc gcctccggcg     1320

tggataatgc agcctccccc cgactggcag acaccgctga acgggattat ttcaccctca     1380

gagagaggct gatcactatg caaaaacaac tggaaggaac ccagaagtat attaatgagc     1440

agtgcagata gagttgccca tatcgatggg caactcatgc aattattgtg agcaatacac     1500

acgcgcttcc agcggagtat aaatgcctaa agtaataaaa ccgagcaatc catttacgaa     1560

tgtttgctgg gtttctgttt taacaacatt ttctgcgccg ccacaaattt tggctgcatc     1620

gacagttttc ttctgcccaa ttccagaaac gaagaaatga tgggtgatgg tttcctttgg     1680

tgctactgct gccggtttgt tttgaacagt aaacgtctgt tgagcacatc ctgtaataag     1740

cagggccagc gcagtagcga gtagcatttt tttcatggtg ttattcccga tgctttttga     1800

agttcgcaga atcgtatgtg tagaaaatta aacaaaccct aaacaatgag ttgaaatttc     1860

atattgttaa tatttattaa tgtatgtcag gtgcgatgaa tcgtcattgt attcccggat     1920

taactatgtc cacagccctg acggggaact tctctgcggg agtgtccggg aataattaaa     1980

acgatgcaca cagggtttag cgcgtacacg tattgcatta tgccaacgcc ccggtgctga     2040

cacggaagaa accggacgtt atgatttagc gtggaaagat ttgtgtagtg ttctgaatgc     2100

tctcagtaaa tagtaatgaa ttatcaaagg tatagtaata tcttttatgt tcatggatat     2160

ttgtaaccca tcggaaaact cctgctttag caagattttc cctgtattgc tgaaatgtga     2220

tttctcttga tttcaaccta tcataggacg tttctataag atgcgtgttt cttgagaatt     2280

taacatttac aaccttttta agtcctttta ttaacacggt gttatcgttt tctaacacga     2340

tgtgaatatt atctgtggct agatagtaaa tataatgtga gacgttgtga cgttttagtt     2400

cagaataaaa caattcacag tctaaatctt ttcgcacttg atcgaatatt tctttaaaaa     2460

tggcaacctg agccattggt aaaaccttcc atgtgatacg agggcgcgta gtttgcatta     2520

tcgtttttat cgtttcaatc tggtctgacc tccttgtgtt ttgttgatga tttatgtcaa     2580

atattaggaa tgttttcact taatagtatt ggttgcgtaa caaagtgcgg tcctgctggc     2640

attctggagg gaaatacaac cgacagatgt atgtaaggcc aacgtgctca aatcttcata     2700

cagaaagatt tgaagtaata ttttaaccgc tagatgaaga gcaagcgcat ggagcgacaa     2760

aatgaataaa gaacaatctg ctgatgatcc ctccgtggat ctgattcgtg taaaaaatat     2820

gcttaatagc accatttcta tgagttaccc tgatgttgta attgcatgta tagaacataa     2880

ggtgtctctg gaagcattca gagcaattga ggcagcgttg gtgaagcacg ataataatat     2940

gaaggattat tccctggtgg ttgactgatc accataactg ctaatcattc aaactattta     3000

gtctgtgaca gagccaacac gcagtctgtc actgtcagga aagtggtaaa actgcaactc     3060

aattactgca atgccctcgt aattaagtga atttacaata tcgtcctgtt cggagggaag     3120

aacgcgggat gttcattctt catcactttt aattgatgta tatgctctct tttctgacgt     3180

tagtctccga cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgctc     3240

aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt     3300

tctgcgggtt ctgttcttcg ttgacatgag gttgccccgt attcagtgtc gctgatttgt     3360

attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc     3420

ataattgatt atttgacgtg gtttgatggc ctccacgcac gttgtgatat gtagatgata     3480

atcattatca ctttacgggt cctttccggt gaaaaaaaag gtaccaaaaa aaacatcgtc     3540

gtgagtagtg aaccgtaagc                                                 3560


<210>  21
<211>  179
<212>  PRT
<213>  Actinia fragacea

<400>  21

Ser Ala Asp Val Ala Gly Ala Val Ile Asp Gly Ala Gly Leu Gly Phe 
1               5                   10                  15      


Asp Val Leu Lys Thr Val Leu Glu Ala Leu Gly Asn Val Lys Arg Lys 
            20                  25                  30          


Ile Ala Val Gly Ile Asp Asn Glu Ser Gly Lys Thr Trp Thr Ala Met 
        35                  40                  45              


Asn Thr Tyr Phe Arg Ser Gly Thr Ser Asp Ile Val Leu Pro His Lys 
    50                  55                  60                  


Val Ala His Gly Lys Ala Leu Leu Tyr Asn Gly Gln Lys Asn Arg Gly 
65                  70                  75                  80  


Pro Val Ala Thr Gly Val Val Gly Val Ile Ala Tyr Ser Met Ser Asp 
                85                  90                  95      


Gly Asn Thr Leu Ala Val Leu Phe Ser Val Pro Tyr Asp Tyr Asn Trp 
            100                 105                 110         


Tyr Ser Asn Trp Trp Asn Val Arg Val Tyr Lys Gly Gln Lys Arg Ala 
        115                 120                 125             


Asp Gln Arg Met Tyr Glu Glu Leu Tyr Tyr His Arg Ser Pro Phe Arg 
    130                 135                 140                 


Gly Asp Asn Gly Trp His Ser Arg Gly Leu Gly Tyr Gly Leu Lys Ser 
145                 150                 155                 160 


Arg Gly Phe Met Asn Ser Ser Gly His Ala Ile Leu Glu Ile His Val 
                165                 170                 175     


Thr Lys Ala 
            


<210>  22
<211>  138
<212>  PRT
<213>  Pleurotus ostreatus

<400>  22

Met Ala Tyr Ala Gln Trp Val Ile Ile Ile Ile His Asn Val Gly Ser 
1               5                   10                  15      


Lys Asp Val Lys Ile Lys Asn Leu Lys Pro Ser Trp Gly Lys Leu His 
            20                  25                  30          


Ala Asp Gly Asp Lys Asp Thr Glu Val Ser Ala Ser Lys Tyr Glu Gly 
        35                  40                  45              


Thr Val Ile Lys Pro Asp Glu Lys Leu Gln Ile Asn Ala Cys Gly Arg 
    50                  55                  60                  


Ser Asp Ala Ala Glu Gly Thr Thr Gly Thr Phe Asp Leu Val Asp Pro 
65                  70                  75                  80  


Ala Asp Gly Asp Lys Gln Val Arg His Phe Tyr Trp Asp Cys Pro Trp 
                85                  90                  95      


Gly Ser Lys Thr Asn Thr Trp Thr Val Ser Gly Ser Asn Thr Lys Trp 
            100                 105                 110         


Met Ile Glu Tyr Ser Gly Gln Asn Leu Asp Ser Gly Ala Leu Gly Thr 
        115                 120                 125             


Ile Thr Val Asp Thr Leu Lys Lys Gly Asn 
    130                 135             


<210>  23
<211>  523
<212>  PRT
<213>  Pleurotus ostreatus

<400>  23

Met Glu Ala Val Leu Ser Arg Gln Ala Ala Thr Ala Glu Ala Ile Gly 
1               5                   10                  15      


Arg Phe Gln Asp Ser Ser Thr Ser Val Gly Leu Val Ala Gly Ser Pro 
            20                  25                  30          


Ser Thr Arg Ile Arg Arg Gln Ala Asp Asn Val Val Leu Lys Ser Thr 
        35                  40                  45              


Ser Gln Ala Gly Asp Thr Leu Asn Asp Val Ile Gln Asp Pro Thr Arg 
    50                  55                  60                  


Arg Asn Lys Leu Ile Asn Asp Asn Asn Leu Leu Lys Gly Ile Ile Met 
65                  70                  75                  80  


Gly Arg Asp Gly Pro Val Pro Ser Ser Arg Glu Leu Ile Val Arg Pro 
                85                  90                  95      


Asp Thr Leu Arg Ala Ile Ile Asn Asn Arg Ala Thr Ile Glu Thr Thr 
            100                 105                 110         


Thr Met Glu Ala Glu Phe Thr Glu Thr Leu Met Glu Ser Asn Tyr Asn 
        115                 120                 125             


Ser Ala Ser Val Lys Val Ser Ala Pro Phe Ile Thr Ala Asn Ser Glu 
    130                 135                 140                 


Tyr Ser Glu Ser Ser Ser Phe Lys Asn Thr Glu Thr Glu Lys Ser Met 
145                 150                 155                 160 


Tyr Thr Ser Ser Arg Tyr Leu Phe Pro Gln Gly Arg Ile Asp Phe Thr 
                165                 170                 175     


Thr Pro Asp Ser Gly Phe Asp Asp Val Ile Lys Leu Ser Pro Gln Phe 
            180                 185                 190         


Thr Ser Gly Val Gln Ala Ala Leu Ala Lys Ala Thr Gly Thr Glu Lys 
        195                 200                 205             


Arg Glu Ala Leu Gln Asn Leu Phe Gln Glu Tyr Gly His Val Phe Arg 
    210                 215                 220                 


Thr Lys Val His Ile Gly Gly Val Leu Ser Ala His Thr Met Glu Thr 
225                 230                 235                 240 


Phe Ser Arg Ser Glu Asn Glu Thr Glu Val Lys Gln Asp Val Lys Ala 
                245                 250                 255     


Gly Leu Glu Gly Ala Val Lys Gly Trp Gly Gly Gly Ala Thr Ala Gly 
            260                 265                 270         


His Gly Asn Thr Gln Gly Thr Ile Thr Thr Ser Gln Asn Arg Lys Leu 
        275                 280                 285             


Asn Val Lys Tyr Ile Val Asn Gly Gly Asp Tyr Thr Lys Ile Gln Asn 
    290                 295                 300                 


Thr Glu Glu Trp Val Ala Ser Thr Asn Gln Ser Glu His Trp Arg Val 
305                 310                 315                 320 


Ile Glu Val Thr Glu Val Thr Ala Val Ala Asp Leu Leu Pro Gln Pro 
                325                 330                 335     


Ile Arg Gly Gln Val Lys Asp Leu Leu Lys Pro Leu Leu Gly Lys Trp 
            340                 345                 350         


Val Asp Val Glu Lys Val Pro Gly Leu Glu Ser Leu Pro Val Ser Val 
        355                 360                 365             


Tyr Arg Pro Lys Gly Ala Ile Pro Ala Gly Trp Phe Trp Leu Gly Asp 
    370                 375                 380                 


Thr Ala Asp Ala Ser Lys Ala Leu Leu Val Lys Pro Thr Leu Pro Ala 
385                 390                 395                 400 


Arg Ser Gly Arg Asn Pro Ala Leu Thr Ser Leu His Gln Gly Ser Gly 
                405                 410                 415     


Met Thr Glu Gln Pro Phe Val Asp Leu Pro Gln Tyr Gln Tyr Leu Ser 
            420                 425                 430         


Thr Tyr Phe Gly Ser Phe Ala His Asp Thr Pro Pro Gly Ser Thr Leu 
        435                 440                 445             


Arg Gly Leu Arg Pro Asp His Val Leu Pro Gly Arg Tyr Glu Met His 
    450                 455                 460                 


Gly Asp Thr Ile Ser Thr Ala Val Tyr Val Thr Arg Pro Val Asp Val 
465                 470                 475                 480 


Pro Phe Pro Glu Asp Glu Cys Phe Asp Leu Lys Ser Leu Val Arg Val 
                485                 490                 495     


Lys Leu Pro Gly Ser Gly Asn Pro Pro Lys Pro Arg Ser Ala Leu Lys 
            500                 505                 510         


Lys Ser Met Val Leu Phe Asp Ser Gly Glu Lys 
        515                 520             


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease-sensitive peptide linker (cleaved by enteropeptidase 
       from E. coli and S. cerevisiae)

<400>  24

Asp Asp Asp Asp Lys 
1               5   


<210>  25
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease-sensitive peptide linker (cleaved by thrombin and factor
       Xa)

<400>  25

Leu Val Pro Arg Gly Ser 
1               5       


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease-sensitive peptide linker (cleaved by TEV protease)

<400>  26

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease-sensitive peptide linker (cleaved by Rhinovirus 3C 
       protease)

<400>  27

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Poly-His tag

<400>  28

His His His His 
1               


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Poly-His tag

<400>  29

His His His His His His 
1               5       


<210>  30
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Poly-His tag

<400>  30

His His His His His His His His 
1               5               


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep-tag

<400>  31

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG-tag

<400>  32

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human influenza hemagglutinin (HA) - tag

<400>  33

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  34
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myc-tag

<400>  34

Glu Gln Lys Leu Ile Ser Glu Glu Asp 
1               5                   


<210>  35
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5-tag

<400>  35

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  36
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide repeating unit

<400>  36
aaaaaagggg gg                                                           12


