                         SEQUENCE LISTING

<110>  Leibniz-Institut fr Neue Materialien gemeinntzige GmbH
 
<120>  Hsp47 stimulierte strukturierte Ablagerung von Kollagen

<130>  INMPT20060WO

<150>  DE 10 2020 126 588.3
<151>  2020-10-09

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  383
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Hsp47 with RDEL sequence

<400>  1

Leu Ser Pro Lys Ala Ala Thr Leu Ala Glu Arg Ser Ala Gly Leu Ala 
1               5                   10                  15      


Phe Ser Leu Tyr Gln Ala Met Ala Lys Asp Gln Ala Val Glu Asn Ile 
            20                  25                  30          


Leu Val Ser Pro Val Val Val Ala Ser Ser Leu Gly Leu Val Ser Leu 
        35                  40                  45              


Gly Gly Lys Ala Thr Thr Ala Ser Gln Ala Lys Ala Val Leu Ser Ala 
    50                  55                  60                  


Glu Gln Leu Arg Asp Glu Glu Val His Ala Gly Leu Gly Glu Leu Leu 
65                  70                  75                  80  


Arg Ser Leu Ser Asn Ser Thr Ala Arg Asn Val Thr Trp Lys Leu Gly 
                85                  90                  95      


Ser Arg Leu Tyr Gly Pro Ser Ser Val Ser Phe Ala Lys Lys Phe Val 
            100                 105                 110         


Arg Ser Ser Lys Gln His Tyr Asn Cys Glu His Ser Lys Ile His Phe 
        115                 120                 125             


Arg Asp Lys Arg Ser Ala Leu Gln Ser Ile Asn Glu Trp Ala Ala Gln 
    130                 135                 140                 


Thr Thr Asp Gly Lys Leu Pro Glu Val Thr Lys Asp Val Glu Arg Thr 
145                 150                 155                 160 


Asp Gly Ala Leu Leu Val Asn Ala Met Phe Phe Lys Pro His Trp Asp 
                165                 170                 175     


Glu Lys Phe His His Lys Met Val Asp Asn Arg Gly Phe Met Val Thr 
            180                 185                 190         


Arg Ser Tyr Thr Val Gly Val Met Met Met His Arg Thr Gly Leu Tyr 
        195                 200                 205             


Asn Tyr Tyr Asp Asp Glu Lys Glu Lys Leu Gln Ile Val Glu Met Pro 
    210                 215                 220                 


Leu Ala His Lys Leu Ser Ser Leu Ile Ile Leu Met Pro His His Val 
225                 230                 235                 240 


Glu Pro Leu Glu Arg Leu Glu Lys Leu Leu Thr Lys Glu Gln Leu Lys 
                245                 250                 255     


Ile Trp Met Gly Lys Met Gln Lys Lys Ala Val Ala Ile Ser Leu Pro 
            260                 265                 270         


Lys Gly Val Val Glu Val Thr His Asp Leu Gln Lys His Leu Ala Gly 
        275                 280                 285             


Leu Gly Leu Thr Glu Ala Ile Asp Lys Asn Lys Ala Asp Leu Ser Arg 
    290                 295                 300                 


Met Ser Gly Lys Lys Asp Leu Tyr Leu Ala Ser Val Phe His Ala Thr 
305                 310                 315                 320 


Ala Phe Glu Leu Asp Thr Asp Gly Asn Pro Phe Asp Gln Asp Ile Tyr 
                325                 330                 335     


Gly Arg Glu Glu Leu Arg Ser Pro Lys Leu Phe Tyr Ala Asp His Pro 
            340                 345                 350         


Phe Ile Phe Leu Val Arg Asp Thr Gln Ser Asp Ser Leu Leu Phe Ile 
        355                 360                 365             


Gly Arg Leu Val Arg Pro Lys Gly Asp Lys Met Arg Asp Glu Leu 
    370                 375                 380             


<210>  2
<211>  383
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Hsp47_mod mith RDEL sequence and modification


<220>
<221>  Group
<222>  (348)..(348)
<223>  Moegliche Position abspaltbare Gruppe

<220>
<221>  Group2
<222>  (350)..(350)
<223>  Moegliche Position abspaltbare Gruppe

<400>  2

Leu Ser Pro Lys Ala Ala Thr Leu Ala Glu Arg Ser Ala Gly Leu Ala 
1               5                   10                  15      


Phe Ser Leu Tyr Gln Ala Met Ala Lys Asp Gln Ala Val Glu Asn Ile 
            20                  25                  30          


Leu Val Ser Pro Val Val Val Ala Ser Ser Leu Gly Leu Val Ser Leu 
        35                  40                  45              


Gly Gly Lys Ala Thr Thr Ala Ser Gln Ala Lys Ala Val Leu Ser Ala 
    50                  55                  60                  


Glu Gln Leu Arg Asp Glu Glu Val His Ala Gly Leu Gly Glu Leu Leu 
65                  70                  75                  80  


Arg Ser Leu Ser Asn Ser Thr Ala Arg Asn Val Thr Trp Lys Leu Gly 
                85                  90                  95      


Ser Arg Leu Tyr Gly Pro Ser Ser Val Ser Phe Ala Lys Lys Phe Val 
            100                 105                 110         


Arg Ser Ser Lys Gln His Tyr Asn Cys Glu His Ser Lys Ile His Phe 
        115                 120                 125             


Arg Asp Lys Arg Ser Ala Leu Gln Ser Ile Asn Glu Trp Ala Ala Gln 
    130                 135                 140                 


Thr Thr Asp Gly Lys Leu Pro Glu Val Thr Lys Asp Val Glu Arg Thr 
145                 150                 155                 160 


Asp Gly Ala Leu Leu Val Asn Ala Met Phe Phe Lys Pro His Trp Asp 
                165                 170                 175     


Glu Lys Phe His His Lys Met Val Asp Asn Arg Gly Phe Met Val Thr 
            180                 185                 190         


Arg Ser Tyr Thr Val Gly Val Met Met Met His Arg Thr Gly Leu Tyr 
        195                 200                 205             


Asn Tyr Tyr Asp Asp Glu Lys Glu Lys Leu Gln Ile Val Glu Met Pro 
    210                 215                 220                 


Leu Ala His Lys Leu Ser Ser Leu Ile Ile Leu Met Pro His His Val 
225                 230                 235                 240 


Glu Pro Leu Glu Arg Leu Glu Lys Leu Leu Thr Lys Glu Gln Leu Lys 
                245                 250                 255     


Ile Trp Met Gly Lys Met Gln Lys Lys Ala Val Ala Ile Ser Leu Pro 
            260                 265                 270         


Lys Gly Val Val Glu Val Thr His Asp Leu Gln Lys His Leu Ala Gly 
        275                 280                 285             


Leu Gly Leu Thr Glu Ala Ile Asp Lys Asn Lys Ala Asp Leu Ser Arg 
    290                 295                 300                 


Met Ser Gly Lys Lys Asp Leu Tyr Leu Ala Ser Val Phe His Ala Thr 
305                 310                 315                 320 


Ala Phe Glu Leu Asp Thr Asp Gly Asn Pro Phe Asp Gln Asp Ile Tyr 
                325                 330                 335     


Gly Arg Glu Glu Leu Arg Ser Pro Lys Leu Phe Tyr Ala Asp His Pro 
            340                 345                 350         


Phe Ile Phe Leu Val Arg Asp Thr Gln Ser Asp Ser Leu Leu Phe Ile 
        355                 360                 365             


Gly Arg Leu Val Arg Pro Lys Gly Asp Lys Met Arg Asp Glu Leu 
    370                 375                 380             


<210>  3
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 1A1 primer1

<400>  3
gaacgcgtgt catcccttgt                                                   20


<210>  4
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 1A1 primer2

<400>  4
gaacgaggta gtctttcagc aaca                                              24


<210>  5
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 3a1 primer1

<400>  5
aacacgcaag gctgtgagac t                                                 21


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 3a1 primer2

<400>  6
gccaacgtcc acaccaaatt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 4a1 primer1

<400>  7
caccctcccc ctttctactc                                                   20


<210>  8
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 4a1 primer2

<400>  8
gcccagagaa tgcacctg                                                     18


<210>  9
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 5A1 primer1

<400>  9
cacaacttgc ctgatggaat aaca                                              24


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 5A1 primer2

<400>  10
gcagggtaca gctgcttggt                                                   20


<210>  11
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 12 primer1

<400>  11
cagtgaggtg gatagtggat ttag                                              24


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Col 12 primer2

<400>  12
agtgttgggt tccttgtgag                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GAPDH primer1

<400>  13
gtcaaggctg agaacgggaa                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GAPDH primer2

<400>  14
gcttcaccac cttcttgatg                                                   20


<210>  15
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hsp47 fill with RDEL sequence

<400>  15

Met Arg Ser Leu Leu Leu Leu Ser Ala Phe Cys Leu Leu Glu Ala Ala 
1               5                   10                  15      


Leu Ala Ala Glu Val Lys Lys Pro Ala Ala Ala Ala Ala Pro Gly Thr 
            20                  25                  30          


Ala Glu Lys Leu Ser Pro Lys Ala Ala Thr Leu Ala Glu Arg Ser Ala 
        35                  40                  45              


Gly Leu Ala Phe Ser Leu Tyr Gln Ala Met Ala Lys Asp Gln Ala Val 
    50                  55                  60                  


Glu Asn Ile Leu Val Ser Pro Val Val Val Ala Ser Ser Leu Gly Leu 
65                  70                  75                  80  


Val Ser Leu Gly Gly Lys Ala Thr Thr Ala Ser Gln Ala Lys Ala Val 
                85                  90                  95      


Leu Ser Ala Glu Gln Leu Arg Asp Glu Glu Val His Ala Gly Leu Gly 
            100                 105                 110         


Glu Leu Leu Arg Ser Leu Ser Asn Ser Thr Ala Arg Asn Val Thr Trp 
        115                 120                 125             


Lys Leu Gly Ser Arg Leu Tyr Gly Pro Ser Ser Val Ser Phe Ala Lys 
    130                 135                 140                 


Lys Phe Val Arg Ser Ser Lys Gln His Tyr Asn Cys Glu His Ser Lys 
145                 150                 155                 160 


Ile His Phe Arg Asp Lys Arg Ser Ala Leu Gln Ser Ile Asn Glu Trp 
                165                 170                 175     


Ala Ala Gln Thr Thr Asp Gly Lys Leu Pro Glu Val Thr Lys Asp Val 
            180                 185                 190         


Glu Arg Thr Asp Gly Ala Leu Leu Val Asn Ala Met Phe Phe Lys Pro 
        195                 200                 205             


His Trp Asp Glu Lys Phe His His Lys Met Val Asp Asn Arg Gly Phe 
    210                 215                 220                 


Met Val Thr Arg Ser Tyr Thr Val Gly Val Met Met Met His Arg Thr 
225                 230                 235                 240 


Gly Leu Tyr Asn Tyr Tyr Asp Asp Glu Lys Glu Lys Leu Gln Ile Val 
                245                 250                 255     


Glu Met Pro Leu Ala His Lys Leu Ser Ser Leu Ile Ile Leu Met Pro 
            260                 265                 270         


His His Val Glu Pro Leu Glu Arg Leu Glu Lys Leu Leu Thr Lys Glu 
        275                 280                 285             


Gln Leu Lys Ile Trp Met Gly Lys Met Gln Lys Lys Ala Val Ala Ile 
    290                 295                 300                 


Ser Leu Pro Lys Gly Val Val Glu Val Thr His Asp Leu Gln Lys His 
305                 310                 315                 320 


Leu Ala Gly Leu Gly Leu Thr Glu Ala Ile Asp Lys Asn Lys Ala Asp 
                325                 330                 335     


Leu Ser Arg Met Ser Gly Lys Lys Asp Leu Tyr Leu Ala Ser Val Phe 
            340                 345                 350         


His Ala Thr Ala Phe Glu Leu Asp Thr Asp Gly Asn Pro Phe Asp Gln 
        355                 360                 365             


Asp Ile Tyr Gly Arg Glu Glu Leu Arg Ser Pro Lys Leu Phe Tyr Ala 
    370                 375                 380                 


Asp His Pro Phe Ile Phe Leu Val Arg Asp Thr Gln Ser Asp Ser Leu 
385                 390                 395                 400 


Leu Phe Ile Gly Arg Leu Val Arg Pro Lys Gly Asp Lys Met Arg Asp 
                405                 410                 415     


Glu Leu 
        


