                         SEQUENCE LISTING

<110>  Mithra recherche et Dveloppement
 
<120>  MEANS AND METHODS FOR THE CONVERSION OF ESTRADIOL TO 
       15ALPHA-HYDROXYESTRADIOL

<130>  MITH-035-PCT

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  410
<212>  PRT
<213>  Nocardia farcinica

<400>  1

Met Asn Ala Cys Pro His Ser Asp Thr Leu Thr Ile Asp Pro Met Ile 
1               5                   10                  15      


Thr Asp Leu Ala Gly Glu Thr Ser Arg Leu Arg Ala Ala Gly Pro Leu 
            20                  25                  30          


Thr Arg Ile Asp Leu Leu Gly Val Pro Ala Leu Ala Val Thr Gly His 
        35                  40                  45              


Thr Leu Ala Arg Gln Leu Leu Thr Asp Thr Arg Leu Val Lys Asp Ile 
    50                  55                  60                  


Asn Ala Trp Ser Leu Trp Gln Ser Gly Thr Val Thr Arg Gln Trp Pro 
65                  70                  75                  80  


Leu Ile Gly Met Ile Asp Val Asp Arg Ser Met Phe Thr Val Asp Gly 
                85                  90                  95      


Pro Glu His Arg Arg Leu Arg Ile Lys Thr Thr Gln Ala Leu Thr Arg 
            100                 105                 110         


Arg Arg Leu Asp Ala Leu Lys Pro Thr Ile Glu Arg Tyr Val Ala Glu 
        115                 120                 125             


Leu Leu Asp Asp Leu Glu Arg Ala Gly Ala Asp Gly Ala Val Val Asp 
    130                 135                 140                 


Leu Lys Ser Val Phe Ala Tyr Pro Leu Pro Met Arg Val Ile Ser Ala 
145                 150                 155                 160 


Leu Met Gly Val Pro Ser Glu Asp Gln Glu Gln Leu Leu Thr Trp Tyr 
                165                 170                 175     


Lys Ala Phe Phe Ser Ile Leu Thr Pro Gln Asp Glu Arg Leu Arg Val 
            180                 185                 190         


Ile Asp Glu Met His Gly Tyr Phe Thr Glu Met Val Arg Arg Lys Thr 
        195                 200                 205             


Ala Glu Pro Gly Asp Asp Leu Thr Ser Ala Leu Ile Tyr Ala Thr Asp 
    210                 215                 220                 


Gly Glu Thr Pro Leu Thr Glu Glu Glu Val Ile Gly Asn Leu Gln Ala 
225                 230                 235                 240 


Leu Val Ala Ala Gly His Glu Thr Thr Val Ser Leu Ile Leu Thr Ala 
                245                 250                 255     


Val Arg Ala Leu Leu Ser His Pro Glu Gln Leu Arg Leu Val Arg Asp 
            260                 265                 270         


Gly Glu Ile Gly Trp Glu Thr Ala Ile Glu Glu Thr Leu Arg Trp Asp 
        275                 280                 285             


Gly Pro Val Ile His Leu Leu Met Arg Phe Ala Thr Glu Asp Ile Asp 
    290                 295                 300                 


Leu Gly Asp Ala Val Ile Pro Arg Gly Glu Gly Val Val Met Ser Tyr 
305                 310                 315                 320 


Arg Ala Ile Gly Arg Asp Ile Thr Val His Gly Ala Asp Ala Asp Asp 
                325                 330                 335     


Phe Asp Ile Thr Arg Ala Thr Ala Ala Arg His Ile Ser Phe Gly His 
            340                 345                 350         


Gly Pro His Ile Cys Pro Gly Ala Ala Leu Ala Arg Leu Glu Ala Ala 
        355                 360                 365             


Ile Ala Leu Pro Ala Leu Phe Thr Arg Phe Pro His Leu His Pro Ala 
    370                 375                 380                 


Leu Pro Leu Asp Gln Ile Pro Asn Leu Pro Val Leu Thr Gln Asn Asp 
385                 390                 395                 400 


Leu Ser His Phe Pro Ile His Leu Gly Arg 
                405                 410 


<210>  2
<211>  421
<212>  PRT
<213>  Nocardia farcinica

<400>  2

Met Asn Gly Gln Ser Ala Thr Thr Ser Ala Gly Gln Ala Pro Glu Thr 
1               5                   10                  15      


Gly Arg Cys Pro Leu Val Ile Asp Pro Met Val Gly Asp Leu Asp Gly 
            20                  25                  30          


Glu Thr Ala Ala Leu Arg Glu Ala Ala Pro Ile Thr Arg Ile Asp Leu 
        35                  40                  45              


Leu Gly Val Pro Ala Trp Thr Val Thr Ser His Ala Glu Ala Arg Arg 
    50                  55                  60                  


Leu Leu Asn Asp Asn Arg Leu Val Lys Asp Ile Asn Arg Trp Glu Leu 
65                  70                  75                  80  


Trp Arg Ser Gly Gln Val Thr His Glu Trp Pro Leu Ile Gly Met Ile 
                85                  90                  95      


Asp Ala Gly Arg Ser Met Phe Thr Val Asp Gly Ala Glu His Arg Arg 
            100                 105                 110         


Leu Arg Ala Lys Thr Ala Gln Ala Val Thr Pro Arg Arg Leu Glu Ala 
        115                 120                 125             


Leu Arg Pro Val Ile Glu Glu Ile Thr Ala Arg Leu Leu Asp Asp Leu 
    130                 135                 140                 


Ala Glu Arg Gly Ala Asp Gly Pro Val Asp Leu Lys Pro Val Phe Ala 
145                 150                 155                 160 


Gln Pro Leu Pro Met Leu Val Val Ser His Leu Met Gly Val Asp Pro 
                165                 170                 175     


Ala Leu His Pro Arg Leu His Glu Leu Tyr Lys Ala Phe Phe Ser Met 
            180                 185                 190         


Leu Thr Pro Gln Asp Glu Arg Leu Ala Val Ile Glu Glu Leu Asp Val 
        195                 200                 205             


Ile Phe Thr Glu Met Val Arg Glu Arg Thr Ala Ala Pro Ala Asp Asp 
    210                 215                 220                 


Leu Thr Ser Ala Leu Ile Leu Ala Asp Glu Gly Gly Glu Pro Leu Thr 
225                 230                 235                 240 


Glu Glu Glu Val Val Gly Asn Leu Lys Ala Met Val Ala Ala Gly His 
                245                 250                 255     


Glu Thr Thr Ile Gly Leu Ile Leu Asn Ala Val Arg Ala Leu Leu Thr 
            260                 265                 270         


His Pro Asp Gln Leu Glu Leu Val Leu Asp Gly Thr Val Gly Trp Asp 
        275                 280                 285             


Thr Val Ile Glu Glu Thr Leu Arg Trp Asp Thr Pro Thr Thr His Leu 
    290                 295                 300                 


Leu Met Arg Phe Ala Thr Glu Asp Ile Gln Val Gly Asp Thr Val Val 
305                 310                 315                 320 


Arg Glu Gly Glu Gly Val Val Val Ser Tyr Arg Ala Ile Gly Arg Asp 
                325                 330                 335     


Arg Ala Gln His Gly Asp Asp Ala Asp Arg Phe Asp Ile Arg Arg Glu 
            340                 345                 350         


Thr Pro Ile Arg His Met Thr Phe Gly His Gly Pro His Ile Cys Pro 
        355                 360                 365             


Gly Ala Ala Leu Ser Arg Leu Glu Ala Ser Ile Ala Leu Pro Ala Leu 
    370                 375                 380                 


Phe Glu Arg Phe Pro Arg Leu Arg Leu Ala Val Pro Val Glu Glu Ile 
385                 390                 395                 400 


Arg Asn Leu Pro Val Leu Thr Gln Asn Asp Leu Ala Ala Phe Pro Val 
                405                 410                 415     


Leu Leu His Gly Ser 
            420     


<210>  3
<211>  414
<212>  PRT
<213>  Nocardia farcinica

<400>  3

Met Asn Cys Pro His Ala Glu Ala Ala Arg Asn Ala Gly Val Ile Ala 
1               5                   10                  15      


Ile Asp Pro Met Val Gln Asp Leu Asp Gly Glu Thr Val Arg Leu Arg 
            20                  25                  30          


Asp Ala Gly Val Leu Ala Arg Ile Asp Leu Leu Gly Val Pro Ala Trp 
        35                  40                  45              


Thr Val Thr Arg His Ala Glu Ala Arg Gln Leu Leu Val Asp Pro Arg 
    50                  55                  60                  


Leu Val Lys Asp Ile Asp Ala Trp Gly Leu Trp Gln Ser Gly Val Val 
65                  70                  75                  80  


Thr Arg Ala Trp Pro Leu Ile Gly Met Ile Asp Ala Gly Arg Ser Met 
                85                  90                  95      


Phe Thr Val Asp Gly Ala Glu His Arg Arg Leu Arg Thr Lys Thr Ser 
            100                 105                 110         


Gln Ala Leu Thr Pro Arg Arg Leu Glu Ala Ile Arg Pro Asp Ile Glu 
        115                 120                 125             


Lys Phe Thr Asp Glu Leu Leu Asp Ala Leu Asp Ala Ala Arg Gly Glu 
    130                 135                 140                 


Asp Gly Val Val Asp Leu Lys Ala Val Phe Ala Gln Pro Leu Pro Met 
145                 150                 155                 160 


Lys Val Val Gly Met Leu Met Gly Val Asp Glu Ser Gln His Ala Met 
                165                 170                 175     


Leu Thr Arg Gln Tyr Lys Ala Phe Phe Ser Met Leu Thr Pro Gln Asp 
            180                 185                 190         


Glu Arg Leu Ala Leu Leu Ala Glu Leu Asp Val Phe Tyr Thr Asp Leu 
        195                 200                 205             


Val Arg Glu Lys Thr Ala Arg Pro Thr Asp Asp Leu Thr Ser Ala Leu 
    210                 215                 220                 


Ile Leu Ala Glu Glu Gly Gly Glu Pro Leu Thr Glu Glu Glu Val Val 
225                 230                 235                 240 


Gly Asn Leu Lys Ala Met Val Ala Ala Gly His Glu Thr Thr Ile Gly 
                245                 250                 255     


Leu Val Leu Asn Ala Val Arg Ala Leu Leu Ser His Pro Asp Gln Leu 
            260                 265                 270         


Arg Leu Val Leu Asp Gly Glu Val Gly Trp Asp Ala Val Val Glu Glu 
        275                 280                 285             


Thr Leu Arg Trp Asp Thr Pro Thr Thr His Leu Leu Met Arg Phe Ala 
    290                 295                 300                 


Thr Glu Asp Ile Ala Val Gly Glu Glu Val Ile Arg Lys Gly Glu Gly 
305                 310                 315                 320 


Val Val Ile Ser Tyr Arg Ala Ile Gly Arg Asp Leu Glu Gln His Gly 
                325                 330                 335     


Pro Asp Ala Asp Ala Phe Asp Ile Thr Arg Pro Thr Arg Asn Arg His 
            340                 345                 350         


Met Thr Phe Gly His Gly Pro His Ile Cys Pro Gly Ala Ala Leu Ser 
        355                 360                 365             


Arg Val Glu Ala Gly Ile Ala Leu Pro Ala Leu Phe Ala Arg Phe Pro 
    370                 375                 380                 


Gly Leu Arg Leu Ala Val Pro Asp Glu Glu Ile Thr Lys Leu Pro Val 
385                 390                 395                 400 


Met Thr Gln Asn Asp Met Ala Ala Phe Pro Val Leu Leu Gly 
                405                 410                 


<210>  4
<211>  18
<212>  PRT
<213>  Nocardia farcinica

<400>  4

Trp Pro Leu Ile Gly Met Ile Asp Val Asp Arg Ser Met Phe Thr Val 
1               5                   10                  15      


Asp Gly 
        


<210>  5
<211>  18
<212>  PRT
<213>  Nocardia farcinica

<400>  5

Asp Gln Ile Pro Asn Leu Pro Val Leu Thr Gln Asn Asp Leu Ser His 
1               5                   10                  15      


Phe Pro 
        


<210>  6
<211>  19
<212>  PRT
<213>  Nocardia farcinica

<400>  6

Trp Pro Leu Ile Gly Met Ile Asp Ala Gly Arg Ser Met Phe Thr Val 
1               5                   10                  15      


Asp Gly Ala 
            


<210>  7
<211>  18
<212>  PRT
<213>  Nocardia farcinica

<400>  7

Glu Glu Ile Arg Asn Leu Pro Val Leu Thr Gln Asn Asp Leu Ala Ala 
1               5                   10                  15      


Phe Pro 
        


<210>  8
<211>  19
<212>  PRT
<213>  Nocardia farcinica

<400>  8

Trp Pro Leu Ile Gly Met Ile Asp Ala Gly Arg Ser Met Phe Thr Val 
1               5                   10                  15      


Asp Gly Ala 
            


<210>  9
<211>  18
<212>  PRT
<213>  Nocardia farcinica

<400>  9

Glu Glu Ile Thr Lys Leu Pro Val Met Thr Gln Asn Asp Met Ala Ala 
1               5                   10                  15      


Phe Pro 
        


<210>  10
<211>  1233
<212>  DNA
<213>  Nocardia farcinica

<400>  10
gtgaacgcct gcccccattc ggacaccctc accatcgacc cgatgatcac cgacctggcc       60

ggggagacca gccgcctgcg cgcggccggc ccgctcacca ggatcgacct gctcggcgtt      120

cccgccctcg ccgtcaccgg ccacacgctg gccaggcagc tgctcaccga caccaggctg      180

gtcaaggaca tcaacgcctg gtcactgtgg cagtccggca cggtgaccag gcagtggccg      240

ctgatcggca tgatcgacgt ggaccgctcg atgttcaccg tggacggccc ggaacaccgc      300

cgactgcgca tcaagaccac ccaggcgctg acccggcgcc gcctcgacgc gctgaagccg      360

accatcgaac gttacgtcgc cgagctgctc gacgacctcg agcgcgcggg cgccgacggc      420

gcggtggtgg atctgaaatc ggtgttcgcc tacccgctgc cgatgcgggt gatcagcgcg      480

ctgatggggg tgccgagcga ggaccaggaa cagctgctca cctggtacaa ggcgttcttc      540

tcgatcctca ccccgcagga cgagcggctg cgggtgatcg acgagatgca cgggtacttc      600

accgagatgg tgcgtcgcaa gaccgccgaa cccggcgacg acctcacctc cgcgctcatc      660

tacgccaccg acggggagac gccgctcacc gaggaggagg tgatcggcaa tctccaggcg      720

ctcgtcgccg cgggccacga aaccaccgtg agcctgatcc tgaccgcggt gcgcgcgctg      780

ctgtcgcatc ccgagcagct gcggctggtc cgcgacggcg agatcggctg ggagaccgcc      840

atcgaagaga ccctgcgctg ggacgggccc gtcatccatc tgctgatgcg cttcgccacc      900

gaggacatcg acctcggcga cgccgtgatc ccgcgcggcg agggcgtggt catgtcctac      960

cgcgcgatcg gccgcgacat caccgtgcac ggtgccgacg ccgacgactt cgacatcacc     1020

cgcgccaccg ccgcccggca catctccttc ggccacggcc cgcacatctg ccccggcgcc     1080

gccctggccc gcctggaagc cgccatcgcc ctccccgccc tgttcacccg cttcccccac     1140

ctccaccccg ccctccccct cgaccagatc cccaacctcc ccgtcctcac ccagaacgac     1200

ctgtcccact tccccatcca cctgggcagg tga                                  1233


<210>  11
<211>  1233
<212>  DNA
<213>  Nocardia farcinica

<400>  11
atgaatgcgt gcccgcattc tgatacattg actattgacc ccatgattac ggatttggcg       60

ggcgaaacat cgcgcctgcg cgccgcagga cctcttacgc gtattgattt attgggcgta      120

cccgcattag cagttaccgg tcacacattg gctcgccagc tgttgacgga tacacgcctg      180

gtaaaagaca ttaacgcatg gtccttgtgg caatccggca ctgttacgcg tcagtggcct      240

cttattggaa tgatcgatgt cgatcgttcc atgtttactg tggacggtcc agaacatcgt      300

cgcttacgca tcaagacaac acaagctctg acgcgtcgcc gtttagacgc tctgaagccg      360

accatcgagc gttacgtcgc cgaattgctt gacgacctgg aacgcgccgg cgcagatgga      420

gccgtcgttg acttgaaatc ggtcttcgcc tacccattac cgatgcgcgt tatctcggcg      480

ttgatgggcg tgcctagtga ggatcaagag cagctgctga catggtataa ggcgtttttt      540

tcaatcctga ccccgcagga tgagcgtttg cgcgtcattg acgaaatgca cggctatttt      600

acagaaatgg tacgccgcaa aactgcagaa cccggcgatg accttacatc tgctcttatt      660

tatgcaactg acggagagac tcctcttacg gaggaagagg taatcggtaa tttacaggca      720

ttggtagccg ccgggcacga aactacagtg agtttgattc tgacagcggt ccgcgcgttg      780

cttagccacc cggaacaatt acgtttagtc cgcgatgggg aaatcggctg ggaaaccgct      840

attgaagaaa ctttgcgctg ggacggccct gtcattcatt tacttatgcg ctttgccacg      900

gaggacattg atcttggcga cgcggttatc ccccgcggcg aaggcgtcgt aatgagttac      960

cgtgcaattg ggcgtgacat tactgtgcat ggcgctgacg ctgatgactt cgacatcacg     1020

cgtgctacgg cggcacgcca tatctcattt ggacatggac ctcacatttg cccgggagcg     1080

gctctggctc gcttggaggc agcgattgcc ttgcccgcgc tgtttactcg ctttcctcac     1140

ttacacccgg ctttaccctt agatcaaatc ccaaatttac ccgtcttgac acaaaacgac     1200

ttgtcgcatt ttccaatcca cttaggtcgc tga                                  1233


