                         SEQUENCE LISTING

<110>  Mojia Biotech Ltd.
       LAU, Man Kit
       LU, Chengliang
       CHIEW, Ansen
 
<120>  IMPROVED PROCESS FOR PRODUCING 1,5-PENTAMETHYLENE DIISOCYANATE 
       FROM CADAVERINE SALT

<130>  19597-10

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2148
<212>  DNA
<213>  Escherichia coli BW25113

<400>  1
atgaacgtta ttgcaatatt gaatcacatg ggggtttatt ttaaagaaga acccatccgt       60

gaacttcatc gcgcgcttga acgtctgaac ttccagattg tttacccgaa cgaccgtgac      120

gacttattaa aactgatcga aaacaatgcg cgtctgtgcg gcgttatttt tgactgggat      180

aaatataatc tcgagctgtg cgaagaaatt agcaaaatga acgagaacct gccgttgtac      240

gcgttcgcta atacgtattc cactctcgat gtaagcctga atgacctgcg tttacagatt      300

agcttctttg aatatgcgct gggtgctgct gaagatattg ctaataagat caagcagacc      360

actgacgaat atatcaacac tattctgcct ccgctgacta aagcactgtt taaatatgtt      420

cgtgaaggta aatatacttt ctgtactcct ggtcacatgg gcggtactgc attccagaaa      480

agcccggtag gtagcctgtt ctatgatttc tttggtccga ataccatgaa atctgatatt      540

tccatttcag tatctgaact gggttctctg ctggatcaca gtggtccaca caaagaagca      600

gaacagtata tcgctcgcgt ctttaacgca gaccgcagct acatggtgac caacggtact      660

tccactgcga acaaaattgt tggtatgtac tctgctccag caggcagcac cattctgatt      720

gaccgtaact gccacaaatc gctgacccac ctgatgatga tgagcgatgt tacgccaatc      780

tatttccgcc cgacccgtaa cgcttacggt attcttggtg gtatcccaca gagtgaattc      840

cagcacgcta ccattgctaa gcgcgtgaaa gaaacaccaa acgcaacctg gccggtacat      900

gctgtaatta ccaactctac ctatgatggt ctgctgtaca acaccgactt catcaagaaa      960

acactggatg tgaaatccat ccactttgac tccgcgtggg tgccttacac caacttctca     1020

ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa agtgatttac     1080

gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat gatccacgtt     1140

aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac caccacttct     1200

ccgcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa aggcaatgca     1260

ggtaagcgtc tgatcaacgg ttctattgaa cgtgcgatca aattccgtaa agagatcaaa     1320

cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga tcatatcgat     1380

acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa aaacatcgat     1440

aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg gatggaaaaa     1500

gacggcacca tgagcgactt tggtattccg gccagcatcg tggcgaaata cctcgacgaa     1560

catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt cagcatcggt     1620

atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgactttaa acgtgcgttc     1680

gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga tcctgaattc     1740

tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat tgttcaccac     1800

aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgacgatggt aatgactccg     1860

tatgctgcat tccagaaaga gctgcacggt atgaccgaag aagtttacct cgacgaaatg     1920

gtaggtcgta ttaacgccaa tatgatcctt ccgtacccgc cgggagttcc tctggtaatg     1980

ccgggtgaaa tgatcaccga agaaagccgt ccggttctgg agttcctgca gatgctgtgt     2040

gaaatcggcg ctcactatcc gggctttgaa accgatattc acggtgcata ccgtcaggct     2100

gatggccgct ataccgttaa ggtattgaaa gaagaaagca aaaaataa                  2148


<210>  2
<211>  715
<212>  PRT
<213>  Escherichia coli BW25113

<400>  2

Met Asn Val Ile Ala Ile Leu Asn His Met Gly Val Tyr Phe Lys Glu 
1               5                   10                  15      


Glu Pro Ile Arg Glu Leu His Arg Ala Leu Glu Arg Leu Asn Phe Gln 
            20                  25                  30          


Ile Val Tyr Pro Asn Asp Arg Asp Asp Leu Leu Lys Leu Ile Glu Asn 
        35                  40                  45              


Asn Ala Arg Leu Cys Gly Val Ile Phe Asp Trp Asp Lys Tyr Asn Leu 
    50                  55                  60                  


Glu Leu Cys Glu Glu Ile Ser Lys Met Asn Glu Asn Leu Pro Leu Tyr 
65                  70                  75                  80  


Ala Phe Ala Asn Thr Tyr Ser Thr Leu Asp Val Ser Leu Asn Asp Leu 
                85                  90                  95      


Arg Leu Gln Ile Ser Phe Phe Glu Tyr Ala Leu Gly Ala Ala Glu Asp 
            100                 105                 110         


Ile Ala Asn Lys Ile Lys Gln Thr Thr Asp Glu Tyr Ile Asn Thr Ile 
        115                 120                 125             


Leu Pro Pro Leu Thr Lys Ala Leu Phe Lys Tyr Val Arg Glu Gly Lys 
    130                 135                 140                 


Tyr Thr Phe Cys Thr Pro Gly His Met Gly Gly Thr Ala Phe Gln Lys 
145                 150                 155                 160 


Ser Pro Val Gly Ser Leu Phe Tyr Asp Phe Phe Gly Pro Asn Thr Met 
                165                 170                 175     


Lys Ser Asp Ile Ser Ile Ser Val Ser Glu Leu Gly Ser Leu Leu Asp 
            180                 185                 190         


His Ser Gly Pro His Lys Glu Ala Glu Gln Tyr Ile Ala Arg Val Phe 
        195                 200                 205             


Asn Ala Asp Arg Ser Tyr Met Val Thr Asn Gly Thr Ser Thr Ala Asn 
    210                 215                 220                 


Lys Ile Val Gly Met Tyr Ser Ala Pro Ala Gly Ser Thr Ile Leu Ile 
225                 230                 235                 240 


Asp Arg Asn Cys His Lys Ser Leu Thr His Leu Met Met Met Ser Asp 
                245                 250                 255     


Val Thr Pro Ile Tyr Phe Arg Pro Thr Arg Asn Ala Tyr Gly Ile Leu 
            260                 265                 270         


Gly Gly Ile Pro Gln Ser Glu Phe Gln His Ala Thr Ile Ala Lys Arg 
        275                 280                 285             


Val Lys Glu Thr Pro Asn Ala Thr Trp Pro Val His Ala Val Ile Thr 
    290                 295                 300                 


Asn Ser Thr Tyr Asp Gly Leu Leu Tyr Asn Thr Asp Phe Ile Lys Lys 
305                 310                 315                 320 


Thr Leu Asp Val Lys Ser Ile His Phe Asp Ser Ala Trp Val Pro Tyr 
                325                 330                 335     


Thr Asn Phe Ser Pro Ile Tyr Glu Gly Lys Cys Gly Met Ser Gly Gly 
            340                 345                 350         


Arg Val Glu Gly Lys Val Ile Tyr Glu Thr Gln Ser Thr His Lys Leu 
        355                 360                 365             


Leu Ala Ala Phe Ser Gln Ala Ser Met Ile His Val Lys Gly Asp Val 
    370                 375                 380                 


Asn Glu Glu Thr Phe Asn Glu Ala Tyr Met Met His Thr Thr Thr Ser 
385                 390                 395                 400 


Pro His Tyr Gly Ile Val Ala Ser Thr Glu Thr Ala Ala Ala Met Met 
                405                 410                 415     


Lys Gly Asn Ala Gly Lys Arg Leu Ile Asn Gly Ser Ile Glu Arg Ala 
            420                 425                 430         


Ile Lys Phe Arg Lys Glu Ile Lys Arg Leu Arg Thr Glu Ser Asp Gly 
        435                 440                 445             


Trp Phe Phe Asp Val Trp Gln Pro Asp His Ile Asp Thr Thr Glu Cys 
    450                 455                 460                 


Trp Pro Leu Arg Ser Asp Ser Thr Trp His Gly Phe Lys Asn Ile Asp 
465                 470                 475                 480 


Asn Glu His Met Tyr Leu Asp Pro Ile Lys Val Thr Leu Leu Thr Pro 
                485                 490                 495     


Gly Met Glu Lys Asp Gly Thr Met Ser Asp Phe Gly Ile Pro Ala Ser 
            500                 505                 510         


Ile Val Ala Lys Tyr Leu Asp Glu His Gly Ile Val Val Glu Lys Thr 
        515                 520                 525             


Gly Pro Tyr Asn Leu Leu Phe Leu Phe Ser Ile Gly Ile Asp Lys Thr 
    530                 535                 540                 


Lys Ala Leu Ser Leu Leu Arg Ala Leu Thr Asp Phe Lys Arg Ala Phe 
545                 550                 555                 560 


Asp Leu Asn Leu Arg Val Lys Asn Met Leu Pro Ser Leu Tyr Arg Glu 
                565                 570                 575     


Asp Pro Glu Phe Tyr Glu Asn Met Arg Ile Gln Glu Leu Ala Gln Asn 
            580                 585                 590         


Ile His Lys Leu Ile Val His His Asn Leu Pro Asp Leu Met Tyr Arg 
        595                 600                 605             


Ala Phe Glu Val Leu Pro Thr Met Val Met Thr Pro Tyr Ala Ala Phe 
    610                 615                 620                 


Gln Lys Glu Leu His Gly Met Thr Glu Glu Val Tyr Leu Asp Glu Met 
625                 630                 635                 640 


Val Gly Arg Ile Asn Ala Asn Met Ile Leu Pro Tyr Pro Pro Gly Val 
                645                 650                 655     


Pro Leu Val Met Pro Gly Glu Met Ile Thr Glu Glu Ser Arg Pro Val 
            660                 665                 670         


Leu Glu Phe Leu Gln Met Leu Cys Glu Ile Gly Ala His Tyr Pro Gly 
        675                 680                 685             


Phe Glu Thr Asp Ile His Gly Ala Tyr Arg Gln Ala Asp Gly Arg Tyr 
    690                 695                 700                 


Thr Val Lys Val Leu Lys Glu Glu Ser Lys Lys 
705                 710                 715 


