                         SEQUENCE LISTING

<110>  GENOMIUM INC
 
<120>  FERMENTATION PROCESS TO PRODUCE BIOACROLEIN AND BIOACRYLIC ACID

<130>  GNM.100XC1PCT

<150>  US 63/085,896
<151>  2020-09-30

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  555
<212>  PRT
<213>  Citrobacter freundii

<400>  1

Met Arg Arg Ser Lys Arg Phe Glu Val Leu Ala Lys Arg Pro Val Asn 
1               5                   10                  15      


Gln Asp Gly Leu Ile Gly Glu Trp Pro Glu Glu Gly Leu Ile Ala Met 
            20                  25                  30          


Glu Ser Pro Tyr Asp Pro Ala Ser Ser Val Lys Val Glu Asn Gly Arg 
        35                  40                  45              


Ile Val Glu Leu Asp Gly Lys Ser Arg Ala Glu Phe Asp Met Ile Asp 
    50                  55                  60                  


Arg Phe Ile Ala Asp Tyr Ala Ile Asn Val Ala Glu Ala Glu His Ala 
65                  70                  75                  80  


Met Gln Leu Asp Ala Leu Glu Ile Ala Arg Met Leu Val Asp Ile His 
                85                  90                  95      


Val Ser Arg Glu Glu Ile Ile Ala Ile Thr Thr Ala Ile Thr Pro Ala 
            100                 105                 110         


Lys Ala Val Glu Val Met Ala Gln Met Asn Val Val Glu Met Met Met 
        115                 120                 125             


Ala Leu Gln Lys Met Arg Ala Arg Arg Thr Pro Ser Asn Gln Cys His 
    130                 135                 140                 


Val Thr Asn Leu Lys Asp Asn Pro Val Gln Ile Ala Ala Asp Ala Ala 
145                 150                 155                 160 


Glu Ala Gly Ile Arg Gly Phe Ser Glu Gln Glu Thr Thr Val Gly Ile 
                165                 170                 175     


Ala Arg Tyr Ala Pro Phe Asn Ala Leu Ala Leu Leu Val Gly Ser Gln 
            180                 185                 190         


Cys Gly Arg Pro Gly Val Leu Thr Gln Cys Ser Val Glu Glu Ala Thr 
        195                 200                 205             


Glu Leu Glu Leu Gly Met Arg Gly Leu Thr Ser Tyr Ala Glu Thr Val 
    210                 215                 220                 


Ser Val Tyr Gly Thr Glu Ser Val Phe Thr Asp Gly Asp Asp Thr Pro 
225                 230                 235                 240 


Trp Ser Lys Ala Phe Leu Ala Ser Ala Tyr Ala Ser Arg Gly Leu Lys 
                245                 250                 255     


Met Arg Tyr Thr Ser Gly Thr Gly Ser Glu Ala Leu Met Gly Tyr Ser 
            260                 265                 270         


Glu Ser Lys Ser Met Leu Tyr Leu Glu Ser Arg Cys Ile Phe Ile Thr 
        275                 280                 285             


Lys Gly Ala Gly Val Gln Gly Leu Gln Asn Gly Ala Val Ser Cys Ile 
    290                 295                 300                 


Gly Met Thr Gly Ala Val Pro Ser Gly Ile Arg Ala Val Leu Ala Glu 
305                 310                 315                 320 


Asn Leu Ile Ala Ser Met Leu Asp Leu Glu Val Ala Ser Ala Asn Asp 
                325                 330                 335     


Gln Thr Phe Ser His Ser Asp Ile Arg Arg Thr Ala Arg Thr Leu Met 
            340                 345                 350         


Gln Met Leu Pro Gly Thr Asp Phe Ile Phe Ser Gly Tyr Ser Ala Val 
        355                 360                 365             


Pro Asn Tyr Asp Asn Met Phe Ala Gly Ser Asn Phe Asp Ala Glu Asp 
    370                 375                 380                 


Phe Asp Asp Tyr Asn Ile Leu Gln Arg Asp Leu Met Val Asp Gly Gly 
385                 390                 395                 400 


Leu Arg Pro Val Thr Glu Glu Glu Thr Ile Ala Ile Arg Asn Lys Ala 
                405                 410                 415     


Ala Arg Ala Ile Gln Ala Val Phe Arg Glu Leu Gly Leu Pro Leu Ile 
            420                 425                 430         


Ser Asp Glu Glu Val Glu Ala Ala Thr Tyr Ala His Gly Ser Lys Asp 
        435                 440                 445             


Met Pro Ala Arg Asn Val Val Glu Asp Leu Ala Ala Val Glu Glu Met 
    450                 455                 460                 


Met Lys Arg Asn Ile Thr Gly Leu Asp Ile Val Gly Ala Leu Ser Cys 
465                 470                 475                 480 


Ser Gly Phe Glu Asp Ile Ala Ser Asn Ile Leu Asn Met Leu Arg Gln 
                485                 490                 495     


Arg Val Thr Gly Asp Tyr Leu Gln Thr Ser Ala Ile Leu Asp Arg Gln 
            500                 505                 510         


Phe Asp Val Val Ser Ala Val Asn Asp Ile Asn Asp Tyr Gln Gly Pro 
        515                 520                 525             


Gly Thr Gly Tyr Arg Ile Ser Ala Glu Arg Trp Ala Glu Ile Lys Asn 
    530                 535                 540                 


Ile Ala Gly Val Val Gln Pro Gly Ser Ile Glu 
545                 550                 555 


<210>  2
<211>  194
<212>  PRT
<213>  Citrobacter freundii

<400>  2

Met Glu Cys Thr Thr Glu Arg Lys Pro Val Phe Thr Leu Gln Val Ser 
1               5                   10                  15      


Glu Gly Glu Ala Ala Lys Ala Asp Asp Arg Ala Asp Glu Val Val Ile 
            20                  25                  30          


Gly Val Gly Pro Ala Phe Gly Lys His Gln His Lys Thr Leu Ile Asp 
        35                  40                  45              


Met Pro His Asn Ala Ile Leu Lys Glu Leu Val Ala Gly Ile Glu Glu 
    50                  55                  60                  


Glu Gly Leu His Ala Arg Val Val Arg Ile Leu Arg Thr Ser Asp Val 
65                  70                  75                  80  


Ser Phe Met Ala Trp Asp Ala Ala Asn Leu Ser Gly Ser Gly Ile Gly 
                85                  90                  95      


Ile Gly Ile Gln Ser Lys Gly Thr Thr Val Ile His Gln Arg Asp Leu 
            100                 105                 110         


Leu Pro Leu Ser Asn Leu Glu Leu Phe Ser Gln Ala Pro Leu Leu Thr 
        115                 120                 125             


Leu Glu Thr Tyr Arg Gln Ile Gly Lys Asn Ala Ala Arg Tyr Ala Arg 
    130                 135                 140                 


Lys Glu Ser Pro Ser Pro Val Pro Val Val Asn Asp Gln Met Val Arg 
145                 150                 155                 160 


Pro Lys Phe Met Ala Lys Ala Ala Leu Phe His Ile Lys Glu Thr Lys 
                165                 170                 175     


His Val Val Gln Asp Ala Ala Pro Val Thr Leu His Ile Ala Leu Val 
            180                 185                 190         


Arg Glu 
        


<210>  3
<211>  144
<212>  PRT
<213>  Citrobacter freundii

<400>  3

Met Thr Met Asn Asp Asn Ile Met Thr Ala Gln Asp Tyr Pro Leu Ala 
1               5                   10                  15      


Thr Arg Cys Pro Glu Lys Ile Leu Thr Pro Thr Gly Lys Pro Leu Thr 
            20                  25                  30          


Asp Ile Thr Leu Glu Asn Val Leu Ala Gly His Val Gly Pro Gln Asp 
        35                  40                  45              


Val Arg Ile Ser Gln Gln Thr Leu Glu Tyr Gln Ala Gln Ile Ala Glu 
    50                  55                  60                  


Gln Met Gln Arg His Ala Val Ala Arg Asn Phe Arg Arg Ala Ala Glu 
65                  70                  75                  80  


Leu Ile Ala Ile Pro Asp Thr Arg Ile Leu Glu Ile Tyr Asn Ala Leu 
                85                  90                  95      


Arg Pro Phe Arg Ser Ser Phe Ala Glu Leu Gln Ala Ile Ala Asp Glu 
            100                 105                 110         


Leu Glu His Thr Trp His Ala Thr Val Asn Ala Gly Phe Val Arg Glu 
        115                 120                 125             


Ser Ala Glu Val Tyr Gln Gln Arg Asn Lys Leu Arg Lys Gly Ser Gln 
    130                 135                 140                 


<210>  4
<211>  603
<212>  PRT
<213>  Citrobacter freundii

<400>  4

Met Pro Leu Ile Ala Gly Ile Asp Ile Gly Asn Ala Thr Thr Glu Val 
1               5                   10                  15      


Ala Leu Ala Gln His Gly Arg Phe Ile Ala Ser Gly Ile Val Ala Thr 
            20                  25                  30          


Thr Gly Met Lys Gly Thr Arg Asp Asn Ile Ala Gly Val Val Ala Ser 
        35                  40                  45              


Leu Gln Gln Ala Leu Glu Lys Thr Pro Trp Pro Leu Asn Asp Val Ala 
    50                  55                  60                  


Lys Ile Cys Ile Asn Glu Ala Ala Pro Val Ile Gly Asp Val Ala Met 
65                  70                  75                  80  


Glu Thr Ile Thr Glu Thr Ile Ile Thr Glu Ser Thr Met Ile Gly His 
                85                  90                  95      


Asn Pro Gln Thr Pro Gly Gly Val Gly Val Gly Met Gly Thr Thr Ile 
            100                 105                 110         


Ala Val Glu Lys Leu Ala Ala Leu Ser Glu Asp Arg Phe Ala Gln Gly 
        115                 120                 125             


Arg Ile Pro Leu Val Gly Glu Glu Met Asp Phe Leu Glu Ala Val Trp 
    130                 135                 140                 


Leu Ile Asn Glu Ala Leu Asp Arg Gly Ile Asn Val Val Ala Ala Ile 
145                 150                 155                 160 


Leu Lys Lys Asp Asp Gly Val Leu Val Asn Asn Arg Leu Arg Arg Thr 
                165                 170                 175     


Met Pro Val Val Asp Glu Val Thr Leu Leu Glu Lys Val Pro Glu Gly 
            180                 185                 190         


Val Leu Ala Ala Val Glu Val Ala Ala Ala Gly Gln Val Val Arg Ala 
        195                 200                 205             


Leu Ser Asn Pro Tyr Gly Ile Ala Thr Phe Phe Ala Leu Thr Pro Glu 
    210                 215                 220                 


Glu Thr Gln Ala Ile Val Pro Ile Ala Arg Ala Leu Ile Gly Asn Arg 
225                 230                 235                 240 


Ser Ala Val Val Leu Lys Thr Pro Gln Gly Asp Val Arg Ser Arg Val 
                245                 250                 255     


Ile Pro Ala Gly Lys Ile Phe Ile Arg Gly Glu Arg Arg Gly Asp Glu 
            260                 265                 270         


Ala Asp Val Ala Gln Gly Ala Gln Ala Ile Met Gln Ala Met Ser Ala 
        275                 280                 285             


Cys Ala Pro Val Cys Asp Ile Arg Gly Glu Ala Gly Thr His Ala Gly 
    290                 295                 300                 


Gly Met Leu Glu Arg Val Arg Lys Val Met Ala Ser Leu Ser Gly His 
305                 310                 315                 320 


Asp Met Asn Ala Val His Ile Gln Asp Leu Leu Ala Val Asp Thr Phe 
                325                 330                 335     


Ile Pro Arg Lys Val Gln Gly Gly Ile Ala Gly Glu Cys Ser Met Glu 
            340                 345                 350         


Asn Ala Val Gly Ile Ala Ala Met Val Lys Ser Asp Arg Leu Gln Met 
        355                 360                 365             


Gln Ala Ile Ala Ser Glu Leu Ser Ala Arg Leu Asn Thr Ala Val Glu 
    370                 375                 380                 


Val Gly Gly Val Glu Ala Asn Met Ala Val Thr Gly Ala Leu Thr Thr 
385                 390                 395                 400 


Pro Gly Cys Ala Ala Pro Leu Ala Ile Leu Asp Leu Gly Ala Gly Ser 
                405                 410                 415     


Thr Asp Ala Ala Ile Ile Asn Ser Glu Gly Ala Val Lys Ala Val His 
            420                 425                 430         


Leu Ala Gly Ala Gly Asn Met Val Ser Leu Leu Ile Gln Thr Glu Leu 
        435                 440                 445             


Gly Leu Ser Asp Pro Phe Leu Ala Glu Glu Ile Lys Lys Tyr Pro Leu 
    450                 455                 460                 


Ala Lys Val Glu Ser Leu Phe Ser Ile Arg His Glu Asn Gly Val Val 
465                 470                 475                 480 


Glu Phe Phe Arg Glu Pro Leu Ser Pro Ser Val Phe Ala Lys Val Val 
                485                 490                 495     


Tyr Leu Lys Asp Gly Glu Leu Ile Pro Val Asp Asn Gln Thr Ser Leu 
            500                 505                 510         


Glu Lys Ile Arg Leu Val Arg Arg Gln Ala Lys Glu Lys Val Phe Val 
        515                 520                 525             


Thr Asn Cys Leu Arg Ala Leu Arg Gln Val Ser Pro Gly Gly Ser Ile 
    530                 535                 540                 


Arg Asp Ile Thr Phe Val Val Leu Val Gly Gly Ser Ser Leu Asp Phe 
545                 550                 555                 560 


Glu Ile Pro Gln Met Ile Thr Asp Ala Leu Ala His Tyr Gly Val Val 
                565                 570                 575     


Ala Gly Gln Gly Asn Ile Arg Gly Thr Glu Gly Pro Arg Asn Ala Val 
            580                 585                 590         


Ala Thr Gly Leu Val Leu Ala Gly Ile Ala Asn 
        595                 600             


<210>  5
<211>  117
<212>  PRT
<213>  Citrobacter freundii

<400>  5

Met Ser Leu Ser Ser Pro Ser Val His Leu Phe Tyr His Pro Arg Trp 
1               5                   10                  15      


Gln Asp Thr Arg Ala Leu Asp Glu Leu Cys Trp Gly Leu Glu Glu Gln 
            20                  25                  30          


Gly Val Pro Cys Arg Thr Val Cys Cys Asp Gly Asn Asp Cys Ala Leu 
        35                  40                  45              


Ala Leu Ser Lys Leu Ala Ala Lys Ser Ser Thr Leu Arg Val Gly Leu 
    50                  55                  60                  


Gly Leu Asn Ala Thr Gly Asp Ile Ala Leu Thr His Ala Gln Leu Pro 
65                  70                  75                  80  


Glu Asp Arg Ala Leu Val Gly Gly His Ile Thr Ala Gly Met Ala Gln 
                85                  90                  95      


Ile Arg Thr Leu Gly Ala Asn Ala Gly Gln Leu Val Lys Val Leu Pro 
            100                 105                 110         


Phe Ser Glu Ile Thr 
        115         


<210>  6
<211>  787
<212>  PRT
<213>  Clostridium butyricum

<400>  6

Met Ile Ser Lys Gly Phe Ser Thr Gln Thr Glu Arg Ile Asn Ile Leu 
1               5                   10                  15      


Lys Ala Gln Ile Leu Asn Ala Lys Pro Cys Val Glu Ser Glu Arg Ala 
            20                  25                  30          


Ile Leu Ile Thr Glu Ser Phe Lys Gln Thr Glu Gly Gln Pro Ala Ile 
        35                  40                  45              


Leu Arg Arg Ala Leu Ala Leu Lys His Ile Leu Glu Asn Ile Pro Ile 
    50                  55                  60                  


Thr Ile Arg Asp Gln Glu Leu Ile Val Gly Ser Leu Thr Lys Glu Pro 
65                  70                  75                  80  


Arg Ser Ser Gln Val Phe Pro Glu Phe Ser Asn Lys Trp Leu Gln Asp 
                85                  90                  95      


Glu Leu Asp Arg Leu Asn Lys Arg Thr Gly Asp Ala Phe Gln Ile Ser 
            100                 105                 110         


Glu Glu Ser Lys Glu Lys Leu Lys Asp Val Phe Glu Tyr Trp Asn Gly 
        115                 120                 125             


Lys Thr Thr Ser Glu Leu Ala Thr Ser Tyr Met Thr Glu Glu Thr Arg 
    130                 135                 140                 


Glu Ala Val Asn Cys Asp Val Phe Thr Val Gly Asn Tyr Tyr Tyr Asn 
145                 150                 155                 160 


Gly Val Gly His Val Ser Val Asp Tyr Gly Lys Val Leu Arg Val Gly 
                165                 170                 175     


Phe Asn Gly Ile Ile Asn Glu Ala Lys Glu Gln Leu Glu Lys Asn Arg 
            180                 185                 190         


Ser Ile Asp Pro Asp Phe Ile Lys Lys Glu Lys Phe Leu Asn Ser Val 
        195                 200                 205             


Ile Ile Ser Cys Glu Ala Ala Ile Thr Tyr Val Asn Arg Tyr Ala Lys 
    210                 215                 220                 


Lys Ala Lys Glu Ile Ala Asp Asn Thr Ser Asp Ala Lys Arg Lys Ala 
225                 230                 235                 240 


Glu Leu Asn Glu Ile Ala Lys Ile Cys Ser Lys Val Ser Gly Glu Gly 
                245                 250                 255     


Ala Lys Ser Phe Tyr Glu Ala Cys Gln Leu Phe Trp Phe Ile His Ala 
            260                 265                 270         


Ile Ile Asn Ile Glu Ser Asn Gly His Ser Ile Ser Pro Ala Arg Phe 
        275                 280                 285             


Asp Gln Tyr Met Tyr Pro Tyr Tyr Glu Asn Asp Lys Asn Ile Thr Asp 
    290                 295                 300                 


Lys Phe Ala Gln Glu Leu Ile Asp Cys Ile Trp Ile Lys Leu Asn Asp 
305                 310                 315                 320 


Ile Asn Lys Val Arg Asp Glu Ile Ser Thr Lys His Phe Gly Gly Tyr 
                325                 330                 335     


Pro Met Tyr Gln Asn Leu Ile Val Gly Gly Gln Asn Ser Glu Gly Lys 
            340                 345                 350         


Asp Ala Thr Asn Lys Val Ser Tyr Met Ala Leu Glu Ala Ala Val His 
        355                 360                 365             


Val Lys Leu Pro Gln Pro Ser Leu Ser Val Arg Ile Trp Asn Lys Thr 
    370                 375                 380                 


Pro Asp Glu Phe Leu Leu Arg Ala Ala Glu Leu Thr Arg Glu Gly Leu 
385                 390                 395                 400 


Gly Leu Pro Ala Tyr Tyr Asn Asp Glu Val Ile Ile Pro Ala Leu Val 
                405                 410                 415     


Ser Arg Gly Leu Thr Leu Glu Asp Ala Arg Asp Tyr Gly Ile Ile Gly 
            420                 425                 430         


Cys Val Glu Pro Gln Lys Pro Gly Lys Thr Glu Gly Trp His Asp Ser 
        435                 440                 445             


Ala Phe Phe Asn Leu Ala Arg Ile Val Glu Leu Thr Ile Asn Ser Gly 
    450                 455                 460                 


Phe Asp Lys Asn Lys Gln Ile Gly Pro Lys Thr Gln Asn Phe Glu Glu 
465                 470                 475                 480 


Met Lys Ser Phe Asp Glu Phe Met Lys Ala Tyr Lys Ala Gln Met Glu 
                485                 490                 495     


Tyr Phe Val Lys His Met Cys Cys Ala Asp Asn Cys Ile Asp Ile Ala 
            500                 505                 510         


His Ala Glu Arg Ala Pro Leu Pro Phe Leu Ser Ser Met Val Asp Asn 
        515                 520                 525             


Cys Ile Gly Lys Gly Lys Ser Leu Gln Asp Gly Gly Ala Glu Tyr Asn 
    530                 535                 540                 


Phe Ser Gly Pro Gln Gly Val Gly Val Ala Asn Ile Gly Asp Ser Leu 
545                 550                 555                 560 


Val Ala Val Lys Lys Ile Val Phe Asp Glu Asn Lys Ile Thr Pro Ser 
                565                 570                 575     


Glu Leu Lys Lys Thr Leu Asn Asn Asp Phe Lys Asn Ser Glu Glu Ile 
            580                 585                 590         


Gln Ala Leu Leu Lys Asn Ala Pro Lys Phe Gly Asn Asp Ile Asp Glu 
        595                 600                 605             


Val Asp Asn Leu Ala Arg Glu Gly Ala Leu Val Tyr Cys Arg Glu Val 
    610                 615                 620                 


Asn Lys Tyr Thr Asn Pro Arg Gly Gly Asn Phe Gln Pro Gly Leu Tyr 
625                 630                 635                 640 


Pro Ser Ser Ile Asn Val Tyr Phe Gly Ser Leu Thr Gly Ala Thr Pro 
                645                 650                 655     


Asp Gly Arg Lys Ser Gly Gln Pro Leu Ala Asp Gly Val Ser Pro Ser 
            660                 665                 670         


Arg Gly Cys Asp Val Ser Gly Pro Thr Ala Ala Cys Asn Ser Val Ser 
        675                 680                 685             


Lys Leu Asp His Phe Ile Ala Ser Asn Gly Thr Leu Phe Asn Gln Lys 
    690                 695                 700                 


Phe His Pro Ser Ala Leu Lys Gly Asp Asn Gly Leu Met Asn Leu Ser 
705                 710                 715                 720 


Ser Leu Ile Arg Ser Tyr Phe Asp Gln Lys Gly Phe His Val Gln Phe 
                725                 730                 735     


Asn Val Ile Asp Lys Lys Ile Leu Leu Ala Ala Gln Lys Asn Pro Glu 
            740                 745                 750         


Lys Tyr Gln Asp Leu Ile Val Arg Val Ala Gly Tyr Ser Ala Gln Phe 
        755                 760                 765             


Ile Ser Leu Asp Lys Ser Ile Gln Asn Asp Ile Ile Ala Arg Thr Glu 
    770                 775                 780                 


His Val Met 
785         


<210>  7
<211>  304
<212>  PRT
<213>  Clostridium butyricum

<400>  7

Met Ser Lys Glu Ile Lys Gly Val Leu Phe Asn Ile Gln Lys Phe Ser 
1               5                   10                  15      


Leu His Asp Gly Pro Gly Ile Arg Thr Ile Val Phe Phe Lys Gly Cys 
            20                  25                  30          


Ser Met Ser Cys Leu Trp Cys Ser Asn Pro Glu Ser Gln Asp Ile Lys 
        35                  40                  45              


Pro Gln Val Met Phe Asn Lys Asn Leu Cys Thr Lys Cys Gly Arg Cys 
    50                  55                  60                  


Lys Ser Gln Cys Lys Ser Ala Ala Ile Asp Met Asn Ser Glu Tyr Arg 
65                  70                  75                  80  


Ile Asp Lys Ser Lys Cys Thr Glu Cys Thr Lys Cys Val Asp Asn Cys 
                85                  90                  95      


Leu Ser Gly Ala Leu Val Ile Glu Gly Arg Asn Tyr Ser Val Glu Asp 
            100                 105                 110         


Val Ile Lys Glu Leu Lys Lys Asp Ser Val Gln Tyr Arg Arg Ser Asn 
        115                 120                 125             


Gly Gly Ile Thr Leu Ser Gly Gly Glu Val Leu Leu Gln Pro Asp Phe 
    130                 135                 140                 


Ala Val Glu Leu Leu Lys Glu Cys Lys Ser Tyr Gly Trp His Thr Ala 
145                 150                 155                 160 


Ile Glu Thr Ala Met Tyr Val Asn Ser Glu Ser Val Lys Lys Val Ile 
                165                 170                 175     


Pro Tyr Ile Asp Leu Ala Met Ile Asp Ile Lys Ser Met Asn Asp Glu 
            180                 185                 190         


Ile His Arg Lys Phe Thr Gly Val Ser Asn Glu Ile Ile Leu Gln Asn 
        195                 200                 205             


Ile Lys Leu Ser Asp Glu Leu Ala Lys Glu Ile Ile Ile Arg Ile Pro 
    210                 215                 220                 


Val Ile Glu Gly Phe Asn Ala Asp Leu Gln Ser Ile Gly Ala Ile Ala 
225                 230                 235                 240 


Gln Phe Ser Lys Ser Leu Thr Asn Leu Lys Arg Ile Asp Leu Leu Pro 
                245                 250                 255     


Tyr His Asn Tyr Gly Glu Asn Lys Tyr Gln Ala Ile Gly Arg Glu Tyr 
            260                 265                 270         


Ser Leu Lys Glu Leu Lys Ser Pro Ser Lys Asp Lys Met Glu Arg Leu 
        275                 280                 285             


Lys Ala Leu Val Glu Ile Met Gly Ile Pro Cys Thr Ile Gly Ala Glu 
    290                 295                 300                 


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer K1

<400>  8
gcgccatggt aagtaaagga tttagtaccc                                        30


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer K2

<400>  9
cgggatccta ttactcagct ccaattgt                                          28


<210>  10
<211>  367
<212>  PRT
<213>  Escherichia coli

<400>  10

Met Asp Arg Ile Ile Gln Ser Pro Gly Lys Tyr Ile Gln Gly Ala Asp 
1               5                   10                  15      


Val Ile Asn Arg Leu Gly Glu Tyr Leu Lys Pro Leu Ala Glu Arg Trp 
            20                  25                  30          


Leu Val Val Gly Asp Lys Phe Val Leu Gly Phe Ala Gln Ser Thr Val 
        35                  40                  45              


Glu Lys Ser Phe Lys Asp Ala Gly Leu Val Val Glu Ile Ala Pro Phe 
    50                  55                  60                  


Gly Gly Glu Cys Ser Gln Asn Glu Ile Asp Arg Leu Arg Gly Ile Ala 
65                  70                  75                  80  


Glu Thr Ala Gln Cys Gly Ala Ile Leu Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      


Leu Asp Thr Ala Lys Ala Leu Ala His Phe Met Gly Val Pro Val Ala 
            100                 105                 110         


Ile Ala Pro Thr Ile Ala Ser Thr Asp Ala Pro Cys Ser Ala Leu Ser 
        115                 120                 125             


Val Ile Tyr Thr Asp Glu Gly Glu Phe Asp Arg Tyr Leu Leu Leu Pro 
    130                 135                 140                 


Asn Asn Pro Asn Met Val Ile Val Asp Thr Lys Ile Val Ala Gly Ala 
145                 150                 155                 160 


Pro Ala Arg Leu Leu Ala Ala Gly Ile Gly Asp Ala Leu Ala Thr Trp 
                165                 170                 175     


Phe Glu Ala Arg Ala Cys Ser Arg Ser Gly Ala Thr Thr Met Ala Gly 
            180                 185                 190         


Gly Lys Cys Thr Gln Ala Ala Leu Ala Leu Ala Glu Leu Cys Tyr Asn 
        195                 200                 205             


Thr Leu Leu Glu Glu Gly Glu Lys Ala Met Leu Ala Ala Glu Gln His 
    210                 215                 220                 


Val Val Thr Pro Ala Leu Glu Arg Val Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 


Ser Gly Val Gly Phe Glu Ser Gly Gly Leu Ala Ala Ala His Ala Val 
                245                 250                 255     


His Asn Gly Leu Thr Ala Ile Pro Asp Ala His His Tyr Tyr His Gly 
            260                 265                 270         


Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Glu Asn Ala 
        275                 280                 285             


Pro Val Glu Glu Ile Glu Thr Val Ala Ala Leu Ser His Ala Val Gly 
    290                 295                 300                 


Leu Pro Ile Thr Leu Ala Gln Leu Asp Ile Lys Glu Asp Val Pro Ala 
305                 310                 315                 320 


Lys Met Arg Ile Val Ala Glu Ala Ala Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     


His Asn Met Pro Gly Gly Ala Thr Pro Asp Gln Val Tyr Ala Ala Leu 
            340                 345                 350         


Leu Val Ala Asp Gln Tyr Gly Gln Arg Phe Leu Gln Glu Trp Glu 
        355                 360                 365         


<210>  11
<211>  356
<212>  PRT
<213>  Escherichia coli

<400>  11

Met Lys Lys Leu Ile Asn Asp Val Gln Asp Val Leu Asp Glu Gln Leu 
1               5                   10                  15      


Ala Gly Leu Ala Lys Ala His Pro Ser Leu Thr Leu His Gln Asp Pro 
            20                  25                  30          


Val Tyr Val Thr Arg Ala Asp Ala Pro Val Ala Gly Lys Val Ala Leu 
        35                  40                  45              


Leu Ser Gly Gly Gly Ser Gly His Glu Pro Met His Cys Gly Tyr Ile 
    50                  55                  60                  


Gly Gln Gly Met Leu Ser Gly Ala Cys Pro Gly Glu Ile Phe Thr Ser 
65                  70                  75                  80  


Pro Thr Pro Asp Lys Ile Phe Glu Cys Ala Met Gln Val Asp Gly Gly 
                85                  90                  95      


Glu Gly Val Leu Leu Ile Ile Lys Asn Tyr Thr Gly Asp Ile Leu Asn 
            100                 105                 110         


Phe Glu Thr Ala Thr Glu Leu Leu His Asp Ser Gly Val Lys Val Thr 
        115                 120                 125             


Thr Val Val Ile Asp Asp Asp Val Ala Val Lys Asp Ser Leu Tyr Thr 
    130                 135                 140                 


Ala Gly Arg Arg Gly Val Ala Asn Thr Val Leu Ile Glu Lys Leu Val 
145                 150                 155                 160 


Gly Ala Ala Ala Glu Arg Gly Asp Ser Leu Asp Ala Cys Ala Glu Leu 
                165                 170                 175     


Gly Arg Lys Leu Asn Asn Gln Gly His Ser Ile Gly Ile Ala Leu Gly 
            180                 185                 190         


Ala Cys Thr Val Pro Ala Ala Gly Lys Pro Ser Phe Thr Leu Ala Asp 
        195                 200                 205             


Asn Glu Met Glu Phe Gly Val Gly Ile His Gly Glu Pro Gly Ile Asp 
    210                 215                 220                 


Arg Arg Pro Phe Ser Ser Leu Asp Gln Thr Val Asp Glu Met Phe Asp 
225                 230                 235                 240 


Thr Leu Leu Val Asn Gly Ser Tyr His Arg Thr Leu Arg Phe Trp Asp 
                245                 250                 255     


Tyr Gln Gln Gly Ser Trp Gln Glu Glu Gln Gln Thr Lys Gln Pro Leu 
            260                 265                 270         


Gln Ser Gly Asp Arg Val Ile Ala Leu Val Asn Asn Leu Gly Ala Thr 
        275                 280                 285             


Pro Leu Ser Glu Leu Tyr Gly Val Tyr Asn Arg Leu Thr Thr Arg Cys 
    290                 295                 300                 


Gln Gln Ala Gly Leu Thr Ile Glu Arg Asn Leu Ile Gly Ala Tyr Cys 
305                 310                 315                 320 


Thr Ser Leu Asp Met Thr Gly Phe Ser Ile Thr Leu Leu Lys Val Asp 
                325                 330                 335     


Asp Glu Thr Leu Ala Leu Trp Asp Ala Pro Val His Thr Pro Ala Leu 
            340                 345                 350         


Asn Trp Gly Lys 
        355     


<210>  12
<211>  370
<212>  PRT
<213>  Escherichia coli

<400>  12

Met Lys Thr Phe Ser Leu Gln Thr Arg Leu Tyr Ser Gly Thr Gly Ser 
1               5                   10                  15      


Leu Asn Ile Leu Ser Arg Phe Thr Asn Arg His Ile Trp Ile Ile Cys 
            20                  25                  30          


Asp Val Phe Leu Ala His Ser Pro Leu Ile Asp Thr Leu His Gln Ala 
        35                  40                  45              


Leu Pro Asp Ser Asn Arg Leu Ser Ile Phe Ser Glu Ile Thr Pro Asp 
    50                  55                  60                  


Pro Thr Ile Gln Thr Val Val Lys Gly Ile Ala Gln Met Gln Thr Leu 
65                  70                  75                  80  


Arg Pro Asp Val Val Ile Gly Phe Gly Gly Gly Ser Ala Leu Asp Ala 
                85                  90                  95      


Ala Lys Ala Ile Val Trp Phe Gly Arg Gln Cys Gly Ile Glu Ile Glu 
            100                 105                 110         


Thr Cys Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr 
        115                 120                 125             


Ser Ala Cys Val Ile Ser Asp Pro Glu Lys Gly Ile Lys Tyr Pro Leu 
    130                 135                 140                 


Phe Asp Asn Ala Leu Tyr Pro Asp Ile Ala Ile Leu Asp Pro Ser Leu 
145                 150                 155                 160 


Val Val Ser Val Pro Pro Ala Ile Thr Ala Asn Thr Gly Met Asp Val 
                165                 170                 175     


Leu Thr His Ala Leu Glu Ala Tyr Val Ser Pro Arg Ala Ser Asp Phe 
            180                 185                 190         


Thr Asp Ala Leu Val Glu Lys Ala Val Gln Ile Val Phe Gln Tyr Leu 
        195                 200                 205             


Pro Thr Ala Val Lys Lys Gly Asp Cys Leu Ala Thr Arg Gly Lys Met 
    210                 215                 220                 


His Asn Ala Ser Thr Leu Ala Gly Ile Ala Phe Ser Gln Ala Gly Leu 
225                 230                 235                 240 


Gly Leu Asn His Ala Leu Ala His Gln Leu Gly Gly Gln Phe His Leu 
                245                 250                 255     


Pro His Gly Leu Ala Asn Ala Leu Leu Leu Thr Ala Val Ile Arg Phe 
            260                 265                 270         


Asn Ala Gly Asp Pro Arg Ala Ala Lys Arg Tyr Ala Arg Leu Ala Lys 
        275                 280                 285             


Thr Cys His Leu Cys Pro Asp Asn Ala Asn Asp Thr Ala Ser Leu Asn 
    290                 295                 300                 


Ala Leu Ile Gln His Ile Glu Gln Leu Lys Thr Thr Cys Thr Leu Pro 
305                 310                 315                 320 


Thr Leu Ala Asn Ala Leu Lys Glu Lys Lys Ala Glu Trp Ser Ile Arg 
                325                 330                 335     


Ile Pro Asp Met Val Gln Ala Ala Leu Ala Asp Ala Thr Leu Arg Thr 
            340                 345                 350         


Asn Pro Arg Ala Ala Asp Ala Ser Ala Ile Ala Glu Leu Leu Glu Glu 
        355                 360                 365             


Leu Leu 
    370 


<210>  13
<211>  495
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Asn Phe His His Leu Ala Tyr Trp Gln Asp Lys Ala Leu Ser Leu 
1               5                   10                  15      


Ala Ile Glu Asn Arg Leu Phe Ile Asn Gly Glu Tyr Thr Ala Ala Ala 
            20                  25                  30          


Glu Asn Glu Thr Phe Glu Thr Val Asp Pro Val Thr Gln Ala Pro Leu 
        35                  40                  45              


Ala Lys Ile Ala Arg Gly Lys Ser Val Asp Ile Asp Arg Ala Val Ser 
    50                  55                  60                  


Ala Ala Arg Gly Val Phe Glu Arg Gly Asp Trp Ser Leu Ser Ser Pro 
65                  70                  75                  80  


Ala Lys Arg Lys Ala Val Leu Asn Lys Leu Ala Asp Leu Met Glu Ala 
                85                  90                  95      


His Ala Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys Pro 
            100                 105                 110         


Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala Ile 
        115                 120                 125             


Arg Trp Tyr Ala Glu Ala Ile Asp Lys Val Tyr Gly Glu Val Ala Thr 
    130                 135                 140                 


Thr Ser Ser His Glu Leu Ala Met Ile Val Arg Glu Pro Val Gly Val 
145                 150                 155                 160 


Ile Ala Ala Ile Val Pro Trp Asn Phe Pro Leu Leu Leu Thr Cys Trp 
                165                 170                 175     


Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Val Leu Lys Pro 
            180                 185                 190         


Ser Glu Lys Ser Pro Leu Ser Ala Ile Arg Leu Ala Gly Leu Ala Lys 
        195                 200                 205             


Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Thr Gly Phe Gly 
    210                 215                 220                 


His Glu Ala Gly Gln Ala Leu Ser Arg His Asn Asp Ile Asp Ala Ile 
225                 230                 235                 240 


Ala Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp Ala 
                245                 250                 255     


Gly Glu Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys Ser 
            260                 265                 270         


Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Lys Ala Ala Ser 
        275                 280                 285             


Ala Thr Ala Ala Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile Ala 
    290                 295                 300                 


Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu Ala 
305                 310                 315                 320 


Leu Leu Lys Gln Gln Ala Gln Asn Trp Gln Pro Gly His Pro Leu Asp 
                325                 330                 335     


Pro Ala Thr Thr Met Gly Thr Leu Ile Asp Ser Ala His Ala Asp Ser 
            340                 345                 350         


Val His Ser Phe Ile Arg Glu Gly Glu Ser Lys Gly Gln Leu Leu Leu 
        355                 360                 365             


Asp Gly Arg Asn Ala Glu Leu Ala Ala Ala Ile Gly Pro Thr Ile Phe 
    370                 375                 380                 


Val Asp Val Asp Pro Asn Ala Ser Leu Ser Arg Glu Glu Ile Phe Gly 
385                 390                 395                 400 


Pro Val Leu Val Val Thr Arg Phe Thr Ser Glu Asp Gln Ala Leu Gln 
                405                 410                 415     


Leu Ala Asn Asp Ser Gln Tyr Gly Leu Gly Ala Ala Val Trp Thr Arg 
            420                 425                 430         


Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly Ser 
        435                 440                 445             


Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe Gly 
    450                 455                 460                 


Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala Leu 
465                 470                 475                 480 


Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ser Leu Glu Ala 
                485                 490                 495 


