                         SEQUENCE LISTING

<110>  Zoetis Services LLC
       United States of America, as represented by the Secretary 
       of Agriculture
       Agin, Tonia S
       Bowersock, Terry
       Briggs, Robert E
       Mattern, Sally
       Moulin, Veronique
       Tatum, Fred M
       Vink, Sarah
 
<120>  Novel Pasteurella multocida Strains and Vaccines having hyaC and 
       nanP Deletions

<130>  ZP000337

<140>  Unknown
<141>  2020-09-30

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  984
<212>  DNA
<213>  P multocida 1062P


<220>
<221>  misc_feature
<223>  wild type nanP

<400>  1
atgaaattta aaaaactact acttgcatct ttatgtttag gtgtttcagc ttctgtattt       60

gcagcagatt acgatcttaa attcggtatg gttgcgggtc caagctcaaa cgaatataaa      120

gcagtagaat tctttgcgaa agaagtgaaa gaaaaatcca atggcaaaat tgatgtggct      180

attttcccta gctcacagtt aggtgatgac cgtgtgatga ttaaacaatt aaaagacggt      240

gcattagact ttacgttagg tgaatcagca cgtttccaaa tttacttccc agaagcagaa      300

gtatttgcgt tgccttatat gattcctaat tttgaaacct ctaaaaaagc gttgctcgac      360

acaaaatttg gtcaaggttt attgaaaaaa attgataaag agttaaacgt acaagtgtta      420

tctgtggcgt ataacggtac acgtcaaaca acttctaacc gtgcaatcaa cagcattgaa      480

gacatgaaag ggttaaaatt acgtgtacct aacgcggcaa ccaaccttgc ttatgcaaaa      540

tacgtgggtg cagcgccaac accaatggca ttctctgaag tttaccttgc gcttcaaaca      600

aactctgtgg atggtcaaga aaacccatta ccgacaatcc aagcacaaaa attctatgaa      660

gtacaaaaat acttagcgtt aactaaccac atcttaaatg accaacttta cttaatcagt      720

aacgatacgt tggcagattt accagaagat ttacaaaaag tggttaaaga tgcagcagcg      780

aaagccgctg aatatcacac taaactcttc gttgacggtg agaacagctt agttgaattc      840

ttcaaaagtc aaggtgtgac agtcacacaa ccagacttaa aaccatttaa agcagcactt      900

acaccatact atgatgaata tctcaagaaa aatggtgaag tcggtaaaat ggcgattgaa      960

gaaatttcta atctcgctaa ataa                                             984


<210>  2
<211>  327
<212>  PRT
<213>  P multocida 1062


<220>
<221>  MISC_FEATURE
<223>  nanP protein

<400>  2

Met Lys Phe Lys Lys Leu Leu Leu Ala Ser Leu Cys Leu Gly Val Ser 
1               5                   10                  15      


Ala Ser Val Phe Ala Ala Asp Tyr Asp Leu Lys Phe Gly Met Val Ala 
            20                  25                  30          


Gly Pro Ser Ser Asn Glu Tyr Lys Ala Val Glu Phe Phe Ala Lys Glu 
        35                  40                  45              


Val Lys Glu Lys Ser Asn Gly Lys Ile Asp Val Ala Ile Phe Pro Ser 
    50                  55                  60                  


Ser Gln Leu Gly Asp Asp Arg Val Met Ile Lys Gln Leu Lys Asp Gly 
65                  70                  75                  80  


Ala Leu Asp Phe Thr Leu Gly Glu Ser Ala Arg Phe Gln Ile Tyr Phe 
                85                  90                  95      


Pro Glu Ala Glu Val Phe Ala Leu Pro Tyr Met Ile Pro Asn Phe Glu 
            100                 105                 110         


Thr Ser Lys Lys Ala Leu Leu Asp Thr Lys Phe Gly Gln Gly Leu Leu 
        115                 120                 125             


Lys Lys Ile Asp Lys Glu Leu Asn Val Gln Val Leu Ser Val Ala Tyr 
    130                 135                 140                 


Asn Gly Thr Arg Gln Thr Thr Ser Asn Arg Ala Ile Asn Ser Ile Glu 
145                 150                 155                 160 


Asp Met Lys Gly Leu Lys Leu Arg Val Pro Asn Ala Ala Thr Asn Leu 
                165                 170                 175     


Ala Tyr Ala Lys Tyr Val Gly Ala Ala Pro Thr Pro Met Ala Phe Ser 
            180                 185                 190         


Glu Val Tyr Leu Ala Leu Gln Thr Asn Ser Val Asp Gly Gln Glu Asn 
        195                 200                 205             


Pro Leu Pro Thr Ile Gln Ala Gln Lys Phe Tyr Glu Val Gln Lys Tyr 
    210                 215                 220                 


Leu Ala Leu Thr Asn His Ile Leu Asn Asp Gln Leu Tyr Leu Ile Ser 
225                 230                 235                 240 


Asn Asp Thr Leu Ala Asp Leu Pro Glu Asp Leu Gln Lys Val Val Lys 
                245                 250                 255     


Asp Ala Ala Ala Lys Ala Ala Glu Tyr His Thr Lys Leu Phe Val Asp 
            260                 265                 270         


Gly Glu Asn Ser Leu Val Glu Phe Phe Lys Ser Gln Gly Val Thr Val 
        275                 280                 285             


Thr Gln Pro Asp Leu Lys Pro Phe Lys Ala Ala Leu Thr Pro Tyr Tyr 
    290                 295                 300                 


Asp Glu Tyr Leu Lys Lys Asn Gly Glu Val Gly Lys Met Ala Ile Glu 
305                 310                 315                 320 


Glu Ile Ser Asn Leu Ala Lys 
                325         


<210>  3
<211>  276
<212>  DNA
<213>  Artificial

<220>
<223>  Delta NanP

<400>  3
atgaaattta aaaaactact acttgcatct ttatgtttag gtgtttcagc ttctgtattt       60

gcagcagatt acgatcttaa attcggtatg gttgcgggtc caagctcaaa cgaatataaa      120

gcagtagaat tcttcaaaag tcaaggtgtg acagtcacac aaccagactt aaaaccattt      180

aaagcagcac ttacaccata ctatgatgaa tatctcaaga aaaatggtga agtcggtaaa      240

atggcgattg aagaaatttc taatctcgct aaataa                                276


<210>  4
<211>  90
<212>  PRT
<213>  Artificial

<220>
<223>  Delta NanP protein

<400>  4

Met Lys Phe Lys Lys Leu Leu Leu Ala Ser Leu Cys Leu Gly Val Ser 
1               5                   10                  15      


Ala Ser Val Phe Ala Ala Asp Tyr Asp Leu Lys Phe Gly Met Val Ala 
            20                  25                  30          


Gly Pro Ser Ser Asn Glu Tyr Lys Ala Val Glu Phe Lys Ser Gln Gly 
        35                  40                  45              


Val Thr Val Thr Gln Pro Asp Leu Lys Pro Phe Lys Ala Ala Leu Thr 
    50                  55                  60                  


Pro Tyr Tyr Asp Glu Tyr Leu Lys Lys Asn Gly Glu Val Gly Lys Met 
65                  70                  75                  80  


Ala Ile Glu Glu Ile Ser Asn Leu Ala Lys 
                85                  90  


<210>  5
<211>  2852
<212>  DNA
<213>  Artificial

<220>
<223>  nanP contained in fragment amplified by primers of SEQ ID NO: 13 
       and 14

<400>  5
gcggatgtga tagttttgac atattaactc cagtctaaat ttatcaaaag aagattgact       60

ccaatttgca taggttaatc ttagaattaa aaaataacaa ccaaaataat aaaaatttga      120

gatctttgtc gcatatttat tcatagggaa tagacagctt aattttagtt atgatttgtc      180

aatccttgct attttttgtg tttgctggtt tgcgatacac tgttctaata ttgctttgag      240

cacttgataa ccttgctcat taaaatgtaa tccgtcggta caaaggcgta aatccagttc      300

accgttagaa tcacaaaagt atttttgtgt ttcaacgtaa gtcacgtctg acggacaatg      360

ttgttttaaa taggtattga gcctgtgaat ttgtgcgtta gtgaccgtat taatctgatt      420

gaccggtgtg gcttctaata aaaagtagtg ggacgtagga gaaatggtgt gtaggtgagt      480

cagaatgtca tttaactatc gcatgacttg cgccggtgaa tacgtttctt ccttacaaat      540

atcattgacg cctaaaaaaa gaaaaacaga ttgtccaagt tgttgaatcc gtttaggttt      600

aacgataaca tccaaatatt gtcgcgtact gacgccagaa agtcctaaat tggcgacggt      660

ttgtcccgct aattgaggtg tgcctgctac ctgttcgtcc cacatgtcaa aaagtgaatg      720

accaattaag ctgatattgg caggtttgga aaattccgcc attttgctct gatagcgttg      780

ataaatatcc tgatcactta gcatgtgtgg acctctattt tgaaataaaa cgctaagtat      840

tatataaaac ctgatatgcc ggtaaacagt aaacttatct tccgtagggg taaatattca      900

attttgtgac gaacctatca tttatgaaat aaaacttcat tttctatata aaaaatagtt      960

ttttcacttt agaatgccaa acgtgtgaaa tttatttcat catcatttta acgtaatccc     1020

aacgtaacca atagaggaga actcataatg aaatttaaaa aactactact tgcatcttta     1080

tgtttaggtg tttcagcttc tgtatttgca gcagattacg atcttaaatt cggtatggtt     1140

gcgggtccaa gctcaaacga atataaagca gtagaattct ttgcgaaaga agtgaaagaa     1200

aaatccaatg gcaaaattga tgtggctatt ttccctagct cacagttagg tgatgaccgt     1260

gtgatgatta aacaattaaa agacggtgca ttagacttta cgttaggtga atcagcacgt     1320

ttccaaattt acttcccaga agcagaagta tttgcgttgc cttatatgat tcctaatttt     1380

gaaacctcta aaaaagcgtt gctcgacaca aaatttggtc aaggtttatt gaaaaaaatt     1440

gataaagagt taaacgtaca agtgttatct gtggcgtata acggtacacg tcaaacaact     1500

tctaaccgtg caatcaacag cattgaagac atgaaagggt taaaattacg tgtacctaac     1560

gcggcaacca accttgctta tgcaaaatac gtgggtgcag cgccaacacc aatggcattc     1620

tctgaagttt accttgcgct tcaaacaaac tctgtggatg gtcaagaaaa cccattaccg     1680

acaatccaag cacaaaaatt ctatgaagta caaaaatact tagcgttaac taaccacatc     1740

ttaaatgacc aactttactt aatcagtaac gatacgttgg cagatttacc agaagattta     1800

caaaaagtgg ttaaagatgc agcagcgaaa gccgctgaat atcacactaa actcttcgtt     1860

gacggtgaga acagcttagt tgaattcttc aaaagtcaag gtgtgacagt cacacaacca     1920

gacttaaaac catttaaagc agcacttaca ccatactatg atgaatatct caagaaaaat     1980

ggtgaagtcg gtaaaatggc gattgaagaa atttctaatc tcgctaaata aatatagtaa     2040

ccttatccct gcgccttaag ggataaggtt cctttttatt gggttgtctt gaggtatcta     2100

tgaaaataat aaataaatta gaagagtgga ttggcggtgt gctattcatt ggaattttct     2160

taattctgtt agcacaaatc attgctcgtc aagtgtttca gtcaccgttt atttggagtg     2220

aagaactcgc aagattgcta tttatctatg tcgggctact tggtatcagc atgggtatcc     2280

gtagtcagca gcatgtttat attgattttt taactaactt tatgcccgag aaagtgagaa     2340

aggtgacaaa ctcctttgtt caagttctca tctttatttc catcattatt ttcattcatt     2400

taggctttaa agtttggatc gactccagtt ttaaaatgga agcgttaact gctttcgctt     2460

cagatttaat tgggcgcgag acgattgtgc ctgaaaaatg gatgtatgcg gcattgcctt     2520

ttatttcttg tttaatgtta ttccgctttt tccaagcgca agttgaaaat tatagaaata     2580

agttaagtta tattcctgtc acggcatttg tgattggtgc ggtcattatt tttgcgattt     2640

tattgattga gccagattgg tataaagtcc tccgtatttc aaattatgtg aaatttggtg     2700

gtgatgcagt gtatatcaca ttagtgattt ggcttgtcat tatgtttgtg ggaaccccgg     2760

taggttggtc attatttatt gcgacgttgc tttattttgc gatgacgcgt tggaatattg     2820

ttaactcggc atcaaccaag ctcaccgaca gt                                   2852


<210>  6
<211>  1793
<212>  DNA
<213>  Artificial

<220>
<223>  Delta HyC and surrounding operons

<400>  6
atgttgataa gaatcatctt acaccagata tcaaaaaaga aatactagcc ttctatcata       60

aacatcaagt gaatatttta ctaaataatg atatctcata ttacacgagt aatagattaa      120

taaaaactga ggcgcattta agtaatatta ataaattaag tcagttaaat ctaaattgtg      180

aatacatcat ttttgataat catgacagcc tattcgttaa aaatgacagc tatgcttata      240

tgaaaaaata tgatgtcggc atgaatttct cagcattaac acatgattgg atcgagaaaa      300

tcaatgcgca tccaccattt aaaaagctca ttaaaactta ttttaatgac aatgacttaa      360

aaagtatgaa tgtgaaaggg gcatcacaag gtatgtttat gacgtatgcg ctagcgcatg      420

agcttctgac gattattaaa gaagtcatca catcttgcca gtcaattgat agtgtgccag      480

aatataacac tgaggatatt tggttccaat ttgcactttt aatcttagaa aagaaaaccg      540

gccatgtatt taataaaaca tcgaccctga cttatatgcc ttgggaacga aaattacaat      600

ggacaaatga acaaattgaa agtgcaaaaa gaggagaaaa tatacctgtt aacaagttca      660

ttattaatag tataactcta taaaacactt gcattttatt aaaaataaaa tcctataata      720

tttgcagttt aaataaagga taaaaaatga agaaaattac aattgctggg gctggctatg      780

ttggtttatc caatgcagta ttattagctc aacaccacaa tgtgatctta ttagatattg      840

atcaaaataa agttgattta attaataata aaaaatcgcc catcacagat aaagaaatcg      900

aagatttctt acaaaataaa tcactgacaa tgatggcaac aacagataaa gaagtggcat      960

taaaaaacgc agactttgtc atcatcgcaa cgccactgca gcaagtttcg gctatggcgg     1020

ttattgttta cccaaagaca ctaaacagtt actggctaac tatgctgacg tacctcaaaa     1080

tctcattgaa gccattgtca aatctaatga aaccagaaaa cgtttcatta ctcatgatgt     1140

attaaataag aaacctaaaa ctgttggtat ttatcgttta atcatgaagt caggttctga     1200

taacttcaga gcttctgcta ttctcgatat tatgccgcat ctcaaagaaa acggtgttga     1260

gattgtgatt tatgagccaa ccttaaatca acaggcattt gaggactacc ccgttattaa     1320

tcaactctct gaatttatta atcgctctga tgtcattctc gctaatcgtt ctgagccaga     1380

tttaaatcaa tgttcccata aaatctatac aagagatatt tttggcggtg atgcttaacc     1440

tgtttaaaat cataaaaaag tatgtgcata ttcaatcttt attacacaaa aaagaatatg     1500

ccttacttta tgctaaatac ataaaccagc tttctatcaa ccagcaggct tatgttattt     1560

gtcaactcaa actctatgat ctctttctga ttgatcctaa atggagccac tctgtttttt     1620

tccagttagg attaattgct cgtggacacg atcatgatag cgatgaagtg gtacgtcgtt     1680

tgatcacttg cactgatttt agcaaaaata agcagttaat cctttctcaa ttacttgctt     1740

attcacctca aattgcaaca acattatgtc cacagacata tcgttatcgt gcg            1793


<210>  7
<211>  2144
<212>  DNA
<213>  Artificial

<220>
<223>  DELTA nanP with surrounding sequences contained in  SEQ ID NO: 5

<400>  7
gcggatgtga tagttttgac atattaactc cagtctaaat ttatcaaaag aagattgact       60

ccaatttgca taggttaatc ttagaattaa aaaataacaa ccaaaataat aaaaatttga      120

gatctttgtc gcatatttat tcatagggaa tagacagctt aattttagtt atgatttgtc      180

aatccttgct attttttgtg tttgctggtt tgcgatacac tgttctaata ttgctttgag      240

cacttgataa ccttgctcat taaaatgtaa tccgtcggta caaaggcgta aatccagttc      300

accgttagaa tcacaaaagt atttttgtgt ttcaacgtaa gtcacgtctg acggacaatg      360

ttgttttaaa taggtattga gcctgtgaat ttgtgcgtta gtgaccgtat taatctgatt      420

gaccggtgtg gcttctaata aaaagtagtg ggacgtagga gaaatggtgt gtaggtgagt      480

cagaatgtca tttaactatc gcatgacttg cgccggtgaa tacgtttctt ccttacaaat      540

atcattgacg cctaaaaaaa gaaaaacaga ttgtccaagt tgttgaatcc gtttaggttt      600

aacgataaca tccaaatatt gtcgcgtact gacgccagaa agtcctaaat tggcgacggt      660

ttgtcccgct aattgaggtg tgcctgctac ctgttcgtcc cacatgtcaa aaagtgaatg      720

accaattaag ctgatattgg caggtttgga aaattccgcc attttgctct gatagcgttg      780

ataaatatcc tgatcactta gcatgtgtgg acctctattt tgaaataaaa cgctaagtat      840

tatataaaac ctgatatgcc ggtaaacagt aaacttatct tccgtagggg taaatattca      900

attttgtgac gaacctatca tttatgaaat aaaacttcat tttctatata aaaaatagtt      960

ttttcacttt agaatgccaa acgtgtgaaa tttatttcat catcatttta acgtaatccc     1020

aacgtaacca atagaggaga actcataatg aaatttaaaa aactactact tgcatcttta     1080

tgtttaggtg tttcagcttc tgtatttgca gcagattacg atcttaaatt cggtatggtt     1140

gcgggtccaa gctcaaacga atataaagca gtagaattct tcaaaagtca aggtgtgaca     1200

gtcacacaac cagacttaaa accatttaaa gcagcactta caccatacta tgatgaatat     1260

ctcaagaaaa atggtgaagt cggtaaaatg gcgattgaag aaatttctaa tctcgctaaa     1320

taaatatagt aaccttatcc ctgcgcctta agggataagg ttccttttta ttgggttgtc     1380

ttgaggtatc tatgaaaata ataaataaat tagaagagtg gattggcggt gtgctattca     1440

ttggaatttt cttaattctg ttagcacaaa tcattgctcg tcaagtgttt cagtcaccgt     1500

ttatttggag tgaagaactc gcaagattgc tatttatcta tgtcgggcta cttggtatca     1560

gcatgggtat ccgtagtcag cagcatgttt atattgattt tttaactaac tttatgcccg     1620

agaaagtgag aaaggtgaca aactcctttg ttcaagttct catctttatt tccatcatta     1680

ttttcattca tttaggcttt aaagtttgga tcgactccag ttttaaaatg gaagcgttaa     1740

ctgctttcgc ttcagattta attgggcgcg agacgattgt gcctgaaaaa tggatgtatg     1800

cggcattgcc ttttatttct tgtttaatgt tattccgctt tttccaagcg caagttgaaa     1860

attatagaaa taagttaagt tatattcctg tcacggcatt tgtgattggt gcggtcatta     1920

tttttgcgat tttattgatt gagccagatt ggtataaagt cctccgtatt tcaaattatg     1980

tgaaatttgg tggtgatgca gtgtatatca cattagtgat ttggcttgtc attatgtttg     2040

tgggaacccc ggtaggttgg tcattattta ttgcgacgtt gctttatttt gcgatgacgc     2100

gttggaatat tgttaactcg gcatcaacca agctcaccga cagt                      2144


<210>  8
<211>  1170
<212>  PRT
<213>  P multocida


<220>
<221>  MISC_FEATURE
<223>  wildtype hyaC

<400>  8

Ala Thr Gly Ala Ala Gly Ala Ala Ala Ala Thr Thr Ala Cys Ala Ala 
1               5                   10                  15      


Thr Thr Gly Cys Thr Gly Gly Gly Gly Cys Thr Gly Gly Cys Thr Ala 
            20                  25                  30          


Thr Gly Thr Thr Gly Gly Thr Thr Thr Ala Thr Cys Cys Ala Ala Thr 
        35                  40                  45              


Gly Cys Ala Gly Thr Ala Thr Thr Ala Thr Thr Ala Gly Cys Thr Cys 
    50                  55                  60                  


Ala Ala Cys Ala Cys Cys Ala Cys Ala Ala Thr Gly Thr Gly Ala Thr 
65                  70                  75                  80  


Cys Thr Thr Ala Thr Thr Ala Gly Ala Thr Ala Thr Thr Gly Ala Thr 
                85                  90                  95      


Cys Ala Ala Ala Ala Thr Ala Ala Ala Gly Thr Thr Gly Ala Thr Thr 
            100                 105                 110         


Thr Ala Ala Thr Thr Ala Ala Thr Ala Ala Thr Ala Ala Ala Ala Ala 
        115                 120                 125             


Ala Thr Cys Gly Cys Cys Cys Ala Thr Cys Ala Cys Ala Gly Ala Thr 
    130                 135                 140                 


Ala Ala Ala Gly Ala Ala Ala Thr Cys Gly Ala Ala Gly Ala Thr Thr 
145                 150                 155                 160 


Thr Cys Thr Thr Ala Cys Ala Ala Ala Ala Thr Ala Ala Ala Thr Cys 
                165                 170                 175     


Ala Cys Thr Gly Ala Cys Ala Ala Thr Gly Ala Thr Gly Gly Cys Ala 
            180                 185                 190         


Ala Cys Ala Ala Cys Ala Gly Ala Thr Ala Ala Ala Gly Ala Ala Gly 
        195                 200                 205             


Thr Gly Gly Cys Ala Thr Thr Ala Ala Ala Ala Ala Ala Cys Gly Cys 
    210                 215                 220                 


Ala Gly Ala Cys Thr Thr Thr Gly Thr Cys Ala Thr Cys Ala Thr Cys 
225                 230                 235                 240 


Gly Cys Ala Ala Cys Gly Cys Cys Ala Ala Cys Ala Gly Ala Cys Thr 
                245                 250                 255     


Ala Thr Ala Ala Thr Ala Cys Cys Gly Ala Ala Ala Cys Ala Gly Gly 
            260                 265                 270         


Thr Thr Ala Thr Thr Thr Thr Ala Ala Thr Ala Cys Ala Thr Cys Cys 
        275                 280                 285             


Ala Cys Thr Gly Thr Thr Gly Ala Ala Gly Cys Thr Gly Thr Cys Ala 
    290                 295                 300                 


Thr Thr Gly Ala Ala Cys Ala Ala Ala Cys Cys Cys Thr Thr Thr Cys 
305                 310                 315                 320 


Ala Ala Thr Cys Ala Ala Thr Cys Cys Ala Cys Ala Ala Gly Cys Ala 
                325                 330                 335     


Ala Cys Gly Ala Thr Thr Ala Thr Thr Ala Thr Ala Ala Ala Ala Thr 
            340                 345                 350         


Cys Ala Ala Cys Gly Ala Thr Thr Cys Cys Cys Gly Thr Thr Gly Gly 
        355                 360                 365             


Thr Thr Thr Thr Ala Cys Cys Gly Ala Ala Ala Ala Ala Ala Thr Gly 
    370                 375                 380                 


Cys Gly Thr Gly Ala Gly Ala Ala Ala Thr Thr Thr Ala Ala Thr Ala 
385                 390                 395                 400 


Cys Cys Cys Cys Ala Ala Ala Thr Cys Thr Thr Ala Thr Cys Thr Thr 
                405                 410                 415     


Thr Thr Cys Ala Cys Cys Thr Gly Ala Ala Thr Thr Thr Cys Thr Ala 
            420                 425                 430         


Ala Gly Ala Gly Ala Gly Gly Gly Ala Ala Ala Ala Gly Cys Cys Cys 
        435                 440                 445             


Thr Thr Thr Ala Cys Gly Ala Thr Ala Ala Thr Thr Thr Gly Thr Ala 
    450                 455                 460                 


Thr Cys Cys Ala Ala Gly Cys Ala Gly Ala Ala Thr Thr Ala Thr Thr 
465                 470                 475                 480 


Gly Thr Thr Gly Gly Cys Ala Gly Thr Ala Cys Thr Thr Cys Thr Thr 
                485                 490                 495     


Ala Thr Cys Ala Ala Gly Cys Ala Ala Ala Ala Gly Thr Ala Thr Thr 
            500                 505                 510         


Thr Gly Cys Cys Gly Ala Thr Ala Thr Gly Thr Thr Ala Ala Cys Ala 
        515                 520                 525             


Cys Ala Gly Thr Gly Thr Gly Cys Cys Ala Gly Ala Ala Ala Ala Ala 
    530                 535                 540                 


Ala Ala Gly Ala Thr Gly Thr Ala Ala Cys Thr Gly Thr Thr Thr Thr 
545                 550                 555                 560 


Ala Thr Thr Thr Ala Cys Ala Cys Ala Cys Ala Ala Thr Ala Cys Thr 
                565                 570                 575     


Gly Ala Gly Gly Cys Cys Gly Ala Ala Gly Cys Thr Gly Thr Thr Ala 
            580                 585                 590         


Ala Ala Thr Thr Ala Thr Thr Thr Gly Cys Ala Ala Ala Thr Ala Cys 
        595                 600                 605             


Gly Thr Ala Thr Cys Thr Cys Gly Cys Ala Ala Thr Gly Cys Gly Ala 
    610                 615                 620                 


Gly Thr Thr Gly Cys Cys Thr Thr Thr Thr Thr Thr Ala Ala Thr Gly 
625                 630                 635                 640 


Ala Ala Thr Thr Ala Gly Ala Thr Ala Cys Thr Thr Ala Thr Gly Cys 
                645                 650                 655     


Gly Ala Gly Thr Cys Thr Thr Cys Ala Cys Cys Ala Thr Thr Thr Ala 
            660                 665                 670         


Ala Ala Thr Ala Cys Ala Ala Ala Ala Gly Ala Cys Ala Thr Thr Ala 
        675                 680                 685             


Thr Cys Ala Ala Thr Gly Gly Thr Ala Thr Thr Thr Cys Thr Ala Cys 
    690                 695                 700                 


Thr Gly Ala Thr Cys Cys Thr Cys Gly Cys Ala Thr Thr Gly Gly Thr 
705                 710                 715                 720 


Ala Cys Ala Cys Ala Cys Thr Ala Cys Ala Ala Thr Ala Ala Cys Cys 
                725                 730                 735     


Cys Ala Ala Gly Thr Thr Thr Cys Gly Gly Cys Thr Ala Thr Gly Gly 
            740                 745                 750         


Cys Gly Gly Thr Thr Ala Thr Thr Gly Thr Thr Thr Ala Cys Cys Cys 
        755                 760                 765             


Ala Ala Ala Gly Ala Cys Ala Cys Thr Ala Ala Ala Cys Ala Gly Thr 
    770                 775                 780                 


Thr Ala Cys Thr Gly Gly Cys Thr Ala Ala Cys Thr Ala Thr Gly Cys 
785                 790                 795                 800 


Thr Gly Ala Cys Gly Thr Ala Cys Cys Thr Cys Ala Ala Ala Ala Thr 
                805                 810                 815     


Cys Thr Cys Ala Thr Thr Gly Ala Ala Gly Cys Cys Ala Thr Thr Gly 
            820                 825                 830         


Thr Cys Ala Ala Ala Thr Cys Thr Ala Ala Thr Gly Ala Ala Ala Cys 
        835                 840                 845             


Cys Ala Gly Ala Ala Ala Ala Cys Gly Thr Thr Thr Cys Ala Thr Thr 
    850                 855                 860                 


Ala Cys Thr Cys Ala Thr Gly Ala Thr Gly Thr Ala Thr Thr Ala Ala 
865                 870                 875                 880 


Ala Thr Ala Ala Gly Ala Ala Ala Cys Cys Thr Ala Ala Ala Ala Cys 
                885                 890                 895     


Thr Gly Thr Thr Gly Gly Thr Ala Thr Thr Thr Ala Thr Cys Gly Thr 
            900                 905                 910         


Thr Thr Ala Ala Thr Cys Ala Thr Gly Ala Ala Gly Thr Cys Ala Gly 
        915                 920                 925             


Gly Thr Thr Cys Thr Gly Ala Thr Ala Ala Cys Thr Thr Cys Ala Gly 
    930                 935                 940                 


Ala Gly Cys Thr Thr Cys Thr Gly Cys Thr Ala Thr Thr Cys Thr Cys 
945                 950                 955                 960 


Gly Ala Thr Ala Thr Thr Ala Thr Gly Cys Cys Gly Cys Ala Thr Cys 
                965                 970                 975     


Thr Cys Ala Ala Ala Gly Ala Ala Ala Ala Cys Gly Gly Thr Gly Thr 
            980                 985                 990         


Thr Gly Ala Gly Ala Thr Thr Gly  Thr Gly Ala Thr Thr  Thr Ala Thr 
        995                 1000                 1005             


Gly Ala  Gly Cys Cys Ala Ala  Cys Cys Thr Thr Ala  Ala Ala Thr 
    1010                 1015                 1020             


Cys Ala  Ala Cys Ala Gly Gly  Cys Ala Thr Thr Thr  Gly Ala Gly 
    1025                 1030                 1035             


Gly Ala  Cys Thr Ala Cys Cys  Cys Cys Gly Thr Thr  Ala Thr Thr 
    1040                 1045                 1050             


Ala Ala  Thr Cys Ala Ala Cys  Thr Cys Thr Cys Thr  Gly Ala Ala 
    1055                 1060                 1065             


Thr Thr  Thr Ala Thr Thr Ala  Ala Thr Cys Gly Cys  Thr Cys Thr 
    1070                 1075                 1080             


Gly Ala  Thr Gly Thr Cys Ala  Thr Thr Cys Thr Cys  Gly Cys Thr 
    1085                 1090                 1095             


Ala Ala  Thr Cys Gly Thr Thr  Cys Thr Gly Ala Gly  Cys Cys Ala 
    1100                 1105                 1110             


Gly Ala  Thr Thr Thr Ala Ala  Ala Thr Cys Ala Ala  Thr Gly Thr 
    1115                 1120                 1125             


Thr Cys  Cys Cys Ala Thr Ala  Ala Ala Ala Thr Cys  Thr Ala Thr 
    1130                 1135                 1140             


Ala Cys  Ala Ala Gly Ala Gly  Ala Thr Ala Thr Thr  Thr Thr Thr 
    1145                 1150                 1155             


Gly Gly  Cys Gly Gly Thr Gly  Ala Thr Gly Cys Thr  
    1160                 1165                 1170 


<210>  9
<211>  390
<212>  PRT
<213>  P multocida


<220>
<221>  MISC_FEATURE
<223>  wildtype hyaC

<400>  9

Met Lys Lys Ile Thr Ile Ala Gly Ala Gly Tyr Val Gly Leu Ser Asn 
1               5                   10                  15      


Ala Val Leu Leu Ala Gln His His Asn Val Ile Leu Leu Asp Ile Asp 
            20                  25                  30          


Gln Asn Lys Val Asp Leu Ile Asn Asn Lys Lys Ser Pro Ile Thr Asp 
        35                  40                  45              


Lys Glu Ile Glu Asp Phe Leu Gln Asn Lys Ser Leu Thr Met Met Ala 
    50                  55                  60                  


Thr Thr Asp Lys Glu Val Ala Leu Lys Asn Ala Asp Phe Val Ile Ile 
65                  70                  75                  80  


Ala Thr Pro Thr Asp Tyr Asn Thr Glu Thr Gly Tyr Phe Asn Thr Ser 
                85                  90                  95      


Thr Val Glu Ala Val Ile Glu Gln Thr Leu Ser Ile Asn Pro Gln Ala 
            100                 105                 110         


Thr Ile Ile Ile Lys Ser Thr Ile Pro Val Gly Phe Thr Glu Lys Met 
        115                 120                 125             


Arg Glu Lys Phe Asn Thr Pro Asn Leu Ile Phe Ser Pro Glu Phe Leu 
    130                 135                 140                 


Arg Glu Gly Lys Ala Leu Tyr Asp Asn Leu Tyr Pro Ser Arg Ile Ile 
145                 150                 155                 160 


Val Gly Ser Thr Ser Tyr Gln Ala Lys Val Phe Ala Asp Met Leu Thr 
                165                 170                 175     


Gln Cys Ala Arg Lys Lys Asp Val Thr Val Leu Phe Thr His Asn Thr 
            180                 185                 190         


Glu Ala Glu Ala Val Lys Leu Phe Ala Asn Thr Tyr Leu Ala Met Arg 
        195                 200                 205             


Val Ala Phe Phe Asn Glu Leu Asp Thr Tyr Ala Ser Leu His His Leu 
    210                 215                 220                 


Asn Thr Lys Asp Ile Ile Asn Gly Ile Ser Thr Asp Pro Arg Ile Gly 
225                 230                 235                 240 


Thr His Tyr Asn Asn Pro Ser Phe Gly Tyr Gly Gly Tyr Cys Leu Pro 
                245                 250                 255     


Lys Asp Thr Lys Gln Leu Leu Ala Asn Tyr Ala Asp Val Pro Gln Asn 
            260                 265                 270         


Leu Ile Glu Ala Ile Val Lys Ser Asn Glu Thr Arg Lys Arg Phe Ile 
        275                 280                 285             


Thr His Asp Val Leu Asn Lys Lys Pro Lys Thr Val Gly Ile Tyr Arg 
    290                 295                 300                 


Leu Ile Met Lys Ser Gly Ser Asp Asn Phe Arg Ala Ser Ala Ile Leu 
305                 310                 315                 320 


Asp Ile Met Pro His Leu Lys Glu Asn Gly Val Glu Ile Val Ile Tyr 
                325                 330                 335     


Glu Pro Thr Leu Asn Gln Gln Ala Phe Glu Asp Tyr Pro Val Ile Asn 
            340                 345                 350         


Gln Leu Ser Glu Phe Ile Asn Arg Ser Asp Val Ile Leu Ala Asn Arg 
        355                 360                 365             


Ser Glu Pro Asp Leu Asn Gln Cys Ser His Lys Ile Tyr Thr Arg Asp 
    370                 375                 380                 


Ile Phe Gly Gly Asp Ala 
385                 390 


<210>  10
<211>  2500
<212>  DNA
<213>  Artificial

<220>
<223>  Wildtype HyaC +744 nt upstream and 544 nt downstream

<400>  10
attattgaat ataataaaaa tatattcgtt attgttctac atgttgataa gaatcatctt       60

acaccagata tcaaaaaaga aatactagcc ttctatcata aacatcaagt gaatatttta      120

ctaaataatg atatctcata ttacacgagt aatagattaa taaaaactga ggcgcattta      180

agtaatatta ataaattaag tcagttaaat ctaaattgtg aatacatcat ttttgataat      240

catgacagcc tattcgttaa aaatgacagc tatgcttata tgaaaaaata tgatgtcggc      300

atgaatttct cagcattaac acatgattgg atcgagaaaa tcaatgcgca tccaccattt      360

aaaaagctca ttaaaactta ttttaatgac aatgacttaa aaagtatgaa tgtgaaaggg      420

gcatcacaag gtatgtttat gacgtatgcg ctagcgcatg agcttctgac gattattaaa      480

gaagtcatca catcttgcca gtcaattgat agtgtgccag aatataacac tgaggatatt      540

tggttccaat ttgcactttt aatcttagaa aagaaaaccg gccatgtatt taataaaaca      600

tcgaccctga cttatatgcc ttgggaacga aaattacaat ggacaaatga acaaattgaa      660

agtgcaaaaa gaggagaaaa tatacctgtt aacaagttca ttattaatag tataactcta      720

taaaacactt gcattttatt aaaaataaaa tcctataata tttgcagttt aaataaagga      780

taaaaaatga agaaaattac aattgctggg gctggctatg ttggtttatc caatgcagta      840

ttattagctc aacaccacaa tgtgatctta ttagatattg atcaaaataa agttgattta      900

attaataata aaaaatcgcc catcacagat aaagaaatcg aagatttctt acaaaataaa      960

tcactgacaa tgatggcaac aacagataaa gaagtggcat taaaaaacgc agactttgtc     1020

atcatcgcaa cgccaacaga ctataatacc gaaacaggtt attttaatac atccactgtt     1080

gaagctgtca ttgaacaaac cctttcaatc aatccacaag caacgattat tataaaatca     1140

acgattcccg ttggttttac cgaaaaaatg cgtgagaaat ttaatacccc aaatcttatc     1200

ttttcacctg aatttctaag agagggaaaa gccctttacg ataatttgta tccaagcaga     1260

attattgttg gcagtacttc ttatcaagca aaagtatttg ccgatatgtt aacacagtgt     1320

gccagaaaaa aagatgtaac tgttttattt acacacaata ctgaggccga agctgttaaa     1380

ttatttgcaa atacgtatct cgcaatgcga gttgcctttt ttaatgaatt agatacttat     1440

gcgagtcttc accatttaaa tacaaaagac attatcaatg gtatttctac tgatcctcgc     1500

attggtacac actacaataa cccaagtttc ggctatggcg gttattgttt acccaaagac     1560

actaaacagt tactggctaa ctatgctgac gtacctcaaa atctcattga agccattgtc     1620

aaatctaatg aaaccagaaa acgtttcatt actcatgatg tattaaataa gaaacctaaa     1680

actgttggta tttatcgttt aatcatgaag tcaggttctg ataacttcag agcttctgct     1740

attctcgata ttatgccgca tctcaaagaa aacggtgttg agattgtgat ttatgagcca     1800

accttaaatc aacaggcatt tgaggactac cccgttatta atcaactctc tgaatttatt     1860

aatcgctctg atgtcattct cgctaatcgt tctgagccag atttaaatca atgttcccat     1920

aaaatctata caagagatat ttttggcggt gatgcttaac ctgtttaaaa tcataaaaaa     1980

gtatgtgcat attcaatctt tattacacaa aaaagaatat gccttacttt atgctaaata     2040

cataaaccag ctttctatca accagcaggc ttatgttatt tgtcaactca aactctatga     2100

tctctttctg attgatccta aatggagcca ctctgttttt ttccagttag gattaattgc     2160

tcgtggacac gatcatgata gcgatgaagt ggtacgtcgt ttgatcactt gcactgattt     2220

tagcaaaaat aagcagttaa tcctttctca attacttgct tattcacctc aaattgcaac     2280

aacattatgt ccacagacat atcgttatcg tgcgctatat ctctcattac tagcgaattt     2340

aaaagacttt gttcgtttaa aagaagaact caataagttg ccgtcatgtg tgttaaagaa     2400

tacacctcat tactgtttgt tacagaattt tgtcgaaaaa gaaaacagca agaaattaga     2460

gaacattaat caatttcttt acttttataa acttggagaa                           2500


<210>  11
<211>  132
<212>  PRT
<213>  Artificial

<220>
<223>  delta HyaC

<400>  11

Met Lys Lys Ile Thr Ile Ala Gly Ala Gly Tyr Val Gly Leu Ser Asn 
1               5                   10                  15      


Ala Val Leu Leu Ala Gln His His Asn Val Ile Leu Leu Asp Ile Asp 
            20                  25                  30          


Gln Asn Lys Val Asp Leu Ile Asn Asn Lys Lys Ser Pro Ile Thr Asp 
        35                  40                  45              


Lys Glu Ile Glu Asp Phe Leu Gln Asn Lys Ser Leu Thr Met Met Ala 
    50                  55                  60                  


Thr Thr Asp Lys Glu Val Ala Leu Lys Asn Ala Asp Phe Val Ile Ile 
65                  70                  75                  80  


Ala Thr Pro Leu Gln Gln Val Ser Ala Met Ala Val Ile Val Tyr Pro 
                85                  90                  95      


Lys Thr Leu Asn Ser Tyr Trp Leu Thr Met Leu Thr Tyr Leu Lys Ile 
            100                 105                 110         


Ser Leu Lys Pro Leu Ser Asn Leu Met Lys Pro Glu Asn Val Ser Leu 
        115                 120                 125             


Leu Met Met Tyr 
    130         


<210>  12
<211>  683
<212>  DNA
<213>  Artificial

<220>
<223>  delta HyaC

<400>  12
atgaagaaaa ttacaattgc tggggctggc tatgttggtt tatccaatgc agtattatta       60

gctcaacacc acaatgtgat cttattagat attgatcaaa ataaagttga tttaattaat      120

aataaaaaat cgcccatcac agataaagaa atcgaagatt tcttacaaaa taaatcactg      180

acaatgatgg caacaacaga taaagaagtg gcattaaaaa acgcagactt tgtcatcatc      240

gcaacgccac aagtttcggc tatggcggtt attgtttacc caaagacact aaacagttac      300

tggctaacta tgctgacgta cctcaaaatc tcattgaagc cattgtcaaa tctaatgaaa      360

ccagaaaacg tttcattact catgatgtat taaataagaa acctaaaact gttggtattt      420

atcgtttaat catgaagtca ggttctgata acttcagagc ttctgctatt ctcgatatta      480

tgccgcatct caaagaaaac ggtgttgaga ttgtgattta tgagccaacc ttaaatcaac      540

aggcatttga ggactacccc gttattaatc aactctctga atttattaat cgctctgatg      600

tcattctcgc taatcgttct gagccagatt taaatcaatg ttcccataaa atctatacaa      660

gagatatttt tggcggtgat gct                                              683


<210>  13
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Bam-nanP-F

<400>  13
aaaggatccg cggatgtgat agttttgaca t                                      31


<210>  14
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Sal-nanP-R

<400>  14
aaagtcgaca ctgtcggtga ccttg                                             25


<210>  15
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Bam-hyaC-F

<400>  15
aaaggatcca tgttgataag aatcatctta cac                                    33


<210>  16
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Pst-hyaC-R

<400>  16
aaactgcagt ggcgttgcga tgatga                                            26


<210>  17
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Pst-hyaC-F

<400>  17
aaactgcagc aagtttcggc tatggcg                                           27


<210>  18
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer 1062 Sal-hyaC-R

<400>  18
aaagctgacc gcacgataac gatatgtctg                                        30


<210>  19
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Partial sequence surrounding DELTAnanP deletion site

<400>  19
aaagcagtag aattcttcaa aagt                                              24


<210>  20
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Partial sequence surrounding DELTAnanP deletion site

<400>  20

Lys Ala Val Glu Phe Phe Lys Ser 
1               5               


<210>  21
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Partial sequence surrounding DELTAhyaC deletion site

<400>  21
acgccactgc agcaagtt                                                     18


<210>  22
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Partial sequence surrounding DELTA hyaC deletion site

<400>  22

Thr Pro Leu Gln Gln Val 
1               5       


