                         SEQUENCE LISTING

<110>  Zoetis Services LLC
       RUAN, Xiaosai
       LIU, Can
       LI, Haiyan
       YU, Hongxin
 
<120>  ATTENUATED PORCINE EPIDEMIC DIARRHEA VIRUS

<130>  ZP000356

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  Fragment of Spike Protein

<400>  1

Tyr Glu Val Phe Glu Lys Val His Val Gln 
1               5                   10  


<210>  2
<211>  1376
<212>  PRT
<213>  Artificial

<220>
<223>  Fragment of PEDV spike protein

<400>  2

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asp Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Ile Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Glu Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Ile Leu Ser Ala Asp  Val Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Gly Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Asp Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Asn 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr Tyr 
    1310                 1315                 1320             


Ile Lys  Trp Pro Trp Trp Val  Trp Leu Ile Ile Phe  Ile Val Leu 
    1325                 1330                 1335             


Ile Phe  Val Val Ser Leu Leu  Val Phe Cys Cys Ile  Ser Thr Gly 
    1340                 1345                 1350             


Cys Cys  Gly Cys Cys Gly Cys  Cys Cys Ala Cys Phe  Ser Gly Cys 
    1355                 1360                 1365             


Cys Arg  Gly Pro Arg Leu Gln  Pro 
    1370                 1375     


<210>  3
<211>  1379
<212>  PRT
<213>  Artificial

<220>
<223>  Mutated Spike protein of PEDV

<400>  3

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asp Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Ile Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Glu Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Ile Leu Ser Ala Asp  Val Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Gly Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Asp Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Lys 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr Tyr 
    1310                 1315                 1320             


Ile Lys  Trp Pro Trp Trp Val  Trp Leu Ile Ile Phe  Ile Val Leu 
    1325                 1330                 1335             


Ile Phe  Val Val Ser Leu Leu  Val Phe Cys Cys Ile  Ser Thr Gly 
    1340                 1345                 1350             


Cys Cys  Gly Cys Cys Gly Cys  Cys Cys Ala Cys Phe  Ser Gly Cys 
    1355                 1360                 1365             


Cys Arg  Gly Pro Arg Leu Gln  Pro Leu Ala Leu 
    1370                 1375                 


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  fragment of mutated Spike protein of PEDV

<400>  4

Gln Pro Leu Ala Leu 
1               5   


<210>  5
<211>  27
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide expressed by newly formed ORF

<400>  5

Met Arg Ser Leu Leu Phe Leu Ile Leu Arg His Phe Leu Ser Ser Met 
1               5                   10                  15      


Val Lys Gln Leu Ile Met Thr Ala Asn Pro Leu 
            20                  25          


<210>  6
<211>  370
<212>  DNA
<213>  Artificial

<220>
<223>  Fragment deleted from ORF-2/ORF-3 of PEDV

<400>  6
acgaagtttt tgaaaaggtc cacgtgcagt gatgtttctt ggactttttc aatacacgat       60

tgacacagtt ttcaaagatg tctcaaagtc tgctaacttg tctttggatg ctgtccaaga      120

gttggagctc aatgtagttc caattagaca agcttcaaat gtgacgggtt ttcttttcac      180

cagtgttttt atctacttct ttgcactgtt taaagcgtct tctttgaggc gcaattatat      240

tatgttggca gcgcgttttg ctgtcattgt tctttattgc ccacttttat attattgtgg      300

tgcattttta gatgcaacta ttatttgttg cacacttatt ggcaggcttt gtttagtctg      360

cttttactcc                                                             370


<210>  7
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Fragment deleted in ORF-3 of PEDV

<400>  7
gttggcatta ctaatgctgc attcta                                            26


<210>  8
<211>  1386
<212>  PRT
<213>  Porcine Pidemic Diarrea virus

<400>  8

Met Lys Ser Leu Thr Tyr Phe Trp Leu Phe Leu Pro Val Leu Ser Thr 
1               5                   10                  15      


Leu Ser Leu Pro Gln Asp Val Thr Arg Cys Ser Ala Asn Thr Asn Phe 
            20                  25                  30          


Arg Arg Phe Phe Ser Lys Phe Asn Val Gln Ala Pro Ala Val Val Val 
        35                  40                  45              


Leu Gly Gly Tyr Leu Pro Ile Gly Glu Asn Gln Gly Val Asn Ser Thr 
    50                  55                  60                  


Trp Tyr Cys Ala Gly Gln His Pro Thr Ala Ser Gly Val His Gly Ile 
65                  70                  75                  80  


Phe Val Ser His Ile Arg Gly Gly His Gly Phe Glu Ile Gly Ile Ser 
                85                  90                  95      


Gln Glu Pro Phe Asp Pro Ser Gly Tyr Gln Leu Tyr Leu His Lys Ala 
            100                 105                 110         


Thr Asn Gly Asn Thr Asn Ala Thr Ala Arg Leu Arg Ile Cys Gln Phe 
        115                 120                 125             


Pro Ser Ile Lys Thr Leu Gly Pro Thr Ala Asn Asn Asp Val Thr Thr 
    130                 135                 140                 


Gly Arg Asn Cys Leu Phe Asn Lys Ala Ile Pro Ala His Met Ser Glu 
145                 150                 155                 160 


His Ser Val Val Gly Ile Thr Trp Asp Asn Asp Arg Val Thr Val Phe 
                165                 170                 175     


Ser Asp Lys Ile Tyr Tyr Phe Tyr Phe Lys Asn Asp Trp Ser Arg Val 
            180                 185                 190         


Ala Thr Lys Cys Tyr Asn Ser Gly Gly Cys Ala Met Gln Tyr Val Tyr 
        195                 200                 205             


Glu Pro Thr Tyr Tyr Met Leu Asn Val Thr Ser Ala Gly Glu Asp Gly 
    210                 215                 220                 


Ile Ser Tyr Gln Pro Cys Thr Ala Asn Cys Ile Gly Tyr Ala Ala Asn 
225                 230                 235                 240 


Val Phe Ala Thr Glu Pro Asn Gly His Ile Pro Glu Gly Phe Ser Phe 
                245                 250                 255     


Asn Asn Trp Phe Leu Leu Ser Asn Asp Ser Thr Leu Val His Gly Lys 
            260                 265                 270         


Val Val Ser Asn Gln Pro Leu Leu Val Asn Cys Leu Leu Ala Ile Pro 
        275                 280                 285             


Lys Ile Tyr Gly Leu Gly Gln Phe Phe Ser Phe Asn Gln Thr Ile Asp 
    290                 295                 300                 


Gly Val Cys Asn Gly Ala Ala Val Gln Arg Ala Pro Glu Ala Leu Arg 
305                 310                 315                 320 


Phe Asn Ile Asn Asp Ile Ser Val Ile Leu Ala Glu Gly Ser Ile Val 
                325                 330                 335     


Leu His Thr Ala Leu Gly Thr Asn Phe Ser Phe Val Cys Ser Asn Ser 
            340                 345                 350         


Ser Asp Pro His Leu Ala Thr Phe Ala Ile Pro Leu Gly Ala Ile Gln 
        355                 360                 365             


Val Pro Tyr Tyr Cys Phe Leu Lys Val Asp Thr Tyr Asn Ser Thr Val 
    370                 375                 380                 


Tyr Lys Phe Leu Ala Val Leu Pro Pro Thr Val Arg Glu Ile Val Ile 
385                 390                 395                 400 


Thr Lys Tyr Gly Asp Val Tyr Val Asn Gly Phe Gly Tyr Leu His Leu 
                405                 410                 415     


Gly Leu Leu Asp Ala Val Thr Ile Asn Phe Thr Gly His Gly Thr Asp 
            420                 425                 430         


Asp Asp Val Ser Gly Phe Trp Thr Ile Ala Ser Thr Asn Phe Val Asp 
        435                 440                 445             


Ala Leu Ile Glu Val Gln Gly Thr Ala Ile Gln Arg Ile Leu Tyr Cys 
    450                 455                 460                 


Asp Asp Pro Val Ser Gln Leu Lys Cys Ser Gln Val Ala Phe Asp Leu 
465                 470                 475                 480 


Asp Asp Gly Phe Tyr Pro Ile Ser Ser Arg Asn Leu Leu Ser His Glu 
                485                 490                 495     


Gln Pro Ile Ser Phe Val Thr Leu Pro Ser Phe Asn Asp His Ser Phe 
            500                 505                 510         


Val Asn Ile Thr Val Ser Ala Ser Phe Gly Gly His Ser Gly Ala Asn 
        515                 520                 525             


Leu Ile Ala Ser Asp Thr Thr Ile Asn Gly Phe Ser Ser Phe Cys Val 
    530                 535                 540                 


Asp Thr Arg Gln Phe Thr Ile Ser Leu Phe Tyr Asn Val Thr Asn Ser 
545                 550                 555                 560 


Tyr Gly Tyr Val Ser Lys Ser Gln Asp Ser Asn Cys Pro Phe Thr Leu 
                565                 570                 575     


Gln Ser Val Asn Asp Tyr Leu Ser Phe Ser Lys Phe Cys Val Ser Thr 
            580                 585                 590         


Ser Leu Leu Ala Ser Ala Cys Thr Ile Asp Leu Phe Gly Tyr Pro Glu 
        595                 600                 605             


Phe Gly Ser Gly Val Lys Phe Thr Ser Leu Tyr Phe Gln Phe Thr Lys 
    610                 615                 620                 


Gly Glu Leu Ile Thr Gly Thr Pro Lys Pro Leu Glu Gly Val Thr Asp 
625                 630                 635                 640 


Val Ser Phe Met Thr Leu Asp Val Cys Thr Glu Tyr Thr Ile Tyr Gly 
                645                 650                 655     


Phe Lys Gly Glu Gly Ile Ile Thr Leu Thr Asn Ser Ser Phe Leu Ala 
            660                 665                 670         


Gly Val Tyr Tyr Thr Ser Asp Ser Gly Gln Leu Leu Ala Phe Lys Asn 
        675                 680                 685             


Val Thr Ser Gly Ala Val Tyr Ser Val Thr Pro Cys Ser Phe Ser Glu 
    690                 695                 700                 


Gln Ala Ala Tyr Val Asp Asp Asp Ile Val Gly Val Ile Ser Ser Leu 
705                 710                 715                 720 


Ser Ser Ser Thr Phe Asn Ser Thr Arg Glu Leu Pro Gly Phe Phe Tyr 
                725                 730                 735     


His Ser Asn Asp Gly Ser Asn Cys Thr Glu Pro Val Leu Val Tyr Ser 
            740                 745                 750         


Asn Ile Gly Val Cys Lys Ser Gly Ser Ile Gly Tyr Val Pro Ser Gln 
        755                 760                 765             


Ser Gly Gln Val Lys Ile Ala Pro Thr Val Thr Gly Asn Ile Ser Ile 
    770                 775                 780                 


Pro Thr Asn Phe Ser Met Ser Ile Arg Thr Glu Tyr Leu Gln Leu Tyr 
785                 790                 795                 800 


Asn Thr Pro Val Ser Val Asp Cys Ala Thr Tyr Val Cys Asn Gly Asn 
                805                 810                 815     


Ser Arg Cys Lys Gln Leu Leu Thr Gln Tyr Thr Ala Ala Cys Lys Thr 
            820                 825                 830         


Ile Glu Ser Ala Leu Gln Leu Ser Ala Arg Leu Glu Ser Val Glu Val 
        835                 840                 845             


Asn Ser Met Leu Thr Ile Ser Glu Glu Ala Leu Gln Leu Ala Thr Ile 
    850                 855                 860                 


Ser Ser Phe Asn Gly Asp Gly Tyr Asn Phe Thr Asn Val Leu Gly Val 
865                 870                 875                 880 


Ser Val Tyr Asp Pro Ala Ser Gly Arg Val Val Gln Lys Arg Ser Phe 
                885                 890                 895     


Ile Glu Asp Leu Leu Phe Asn Lys Val Val Thr Asn Gly Leu Gly Thr 
            900                 905                 910         


Val Asp Glu Asp Tyr Lys Arg Cys Ser Asn Gly Arg Ser Val Ala Asp 
        915                 920                 925             


Leu Val Cys Ala Gln Tyr Tyr Ser Gly Val Met Val Leu Pro Gly Val 
    930                 935                 940                 


Val Asp Ala Glu Lys Leu His Met Tyr Ser Ala Ser Leu Ile Gly Gly 
945                 950                 955                 960 


Met Val Leu Gly Gly Phe Thr Ser Ala Ala Ala Leu Pro Phe Ser Tyr 
                965                 970                 975     


Ala Val Gln Ala Arg Leu Asn Tyr Leu Ala Leu Gln Thr Asp Val Leu 
            980                 985                 990         


Gln Arg Asn Gln Gln Leu Leu Ala  Glu Ser Phe Asn Ser  Ala Ile Gly 
        995                 1000                 1005             


Asn Ile  Thr Ser Ala Phe Glu  Ser Val Lys Glu Ala  Ile Ser Gln 
    1010                 1015                 1020             


Thr Ser  Lys Gly Leu Asn Thr  Val Ala His Ala Leu  Thr Lys Val 
    1025                 1030                 1035             


Gln Glu  Val Val Asn Ser Gln  Gly Ala Ala Leu Thr  Gln Leu Thr 
    1040                 1045                 1050             


Val Gln  Leu Gln His Asn Phe  Gln Ala Ile Ser Ser  Ser Ile Asp 
    1055                 1060                 1065             


Asp Ile  Tyr Ser Arg Leu Asp  Ile Leu Ser Ala Asp  Val Gln Val 
    1070                 1075                 1080             


Asp Arg  Leu Ile Thr Gly Arg  Leu Ser Ala Leu Asn  Ala Phe Val 
    1085                 1090                 1095             


Ala Gln  Thr Leu Thr Lys Tyr  Thr Glu Val Gln Ala  Ser Arg Lys 
    1100                 1105                 1110             


Leu Ala  Gln Gln Lys Val Asn  Glu Cys Val Lys Ser  Gln Ser Gln 
    1115                 1120                 1125             


Arg Tyr  Gly Phe Cys Gly Gly  Asp Gly Glu His Ile  Phe Ser Leu 
    1130                 1135                 1140             


Val Gln  Ala Ala Pro Gln Gly  Leu Leu Phe Leu His  Thr Val Leu 
    1145                 1150                 1155             


Val Pro  Gly Asp Phe Val Asp  Val Ile Ala Ile Ala  Gly Leu Cys 
    1160                 1165                 1170             


Val Asn  Asp Glu Ile Ala Leu  Thr Leu Arg Glu Pro  Gly Leu Val 
    1175                 1180                 1185             


Leu Phe  Thr His Glu Leu Gln  Asn His Thr Ala Thr  Glu Tyr Phe 
    1190                 1195                 1200             


Val Ser  Ser Arg Arg Met Phe  Glu Pro Arg Lys Pro  Thr Val Ser 
    1205                 1210                 1215             


Asp Phe  Val Gln Ile Glu Ser  Cys Val Val Thr Tyr  Val Asn Leu 
    1220                 1225                 1230             


Thr Arg  Asp Gln Leu Pro Asp  Val Ile Pro Asp Tyr  Ile Asp Val 
    1235                 1240                 1245             


Asn Lys  Thr Leu Asp Glu Ile  Leu Ala Ser Leu Pro  Asn Arg Thr 
    1250                 1255                 1260             


Gly Pro  Ser Leu Pro Leu Asp  Val Phe Asn Ala Thr  Tyr Leu Asn 
    1265                 1270                 1275             


Leu Thr  Gly Glu Ile Ala Asp  Leu Glu Gln Arg Ser  Glu Ser Leu 
    1280                 1285                 1290             


Arg Asn  Thr Thr Glu Glu Leu  Gln Ser Leu Ile Tyr  Asn Ile Asn 
    1295                 1300                 1305             


Asn Thr  Leu Val Asp Leu Glu  Trp Leu Asn Arg Val  Glu Thr Tyr 
    1310                 1315                 1320             


Ile Lys  Trp Pro Trp Trp Val  Trp Leu Ile Ile Phe  Ile Val Leu 
    1325                 1330                 1335             


Ile Phe  Val Val Ser Leu Leu  Val Phe Cys Cys Ile  Ser Thr Gly 
    1340                 1345                 1350             


Cys Cys  Gly Cys Cys Gly Cys  Cys Cys Ala Cys Phe  Ser Gly Cys 
    1355                 1360                 1365             


Cys Arg  Gly Pro Arg Leu Gln  Pro Tyr Glu Val Phe  Glu Lys Val 
    1370                 1375                 1380             


His Val  Gln 
    1385     


<210>  9
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  New Orf created as a result of mutations in PEDV

<400>  9
atgcgctctt tattattttt aatactacga cactttcttt cctcaatggt aaagcagctt       60

attatgacgg caaatccatt gtga                                              84


<210>  10
<211>  27642
<212>  DNA
<213>  Artificial

<220>
<223>  Full genome of mutated PEDV virus

<400>  10
acttaaagag attttctatc tatggatagt tagctctttt tctagactct tgtctactca       60

attcaactaa acgaaatttt gtccttccgg ccgcatgtcc atgctgctgg aagctgacgt      120

ggaatttcat taggtttgct taagtagcca tcgcaagtgc tgtgctgtcc tctagttcct      180

ggttggcgtt ccgtcgcctt ctacatacta gacaaacagc catcctccgg ttccgtctgg      240

gggttgcgtg gataactagt tccgtctagt ttgaaaccag taactgtcgg ctatggctag      300

caaccaagtc acattggctt ttgccaatga tgcagaaatt tcagcttttg gcttttgcac      360

tgctagtgaa gccgtctcat actattctga ggccgccgct agtggattta tgcaatgccg      420

tttcgtgtcc ttcgatctcg ctgacactgt tgagggattg cttcccgaag actatgtcat      480

ggtggtggtc ggcactacca agcttagtgc gtatgtggac acttttggta gccgccccag      540

aaacatttgt ggttggctgt tattttctaa ctgtaattac ttcctcgaag agttagagct      600

cacttttggt cgtcgtggtg gtaacatcgt gccagttgac caatacatgt gtggcgctga      660

cgggaaacct gttcttcagg aatccgagtg ggagtataca gacttttttg ctgactccga      720

agacggtcaa ctcaacattg ctgggatcac ttatgtgaag gcctggattg tagagcgatc      780

ggacgtctct tatgcgagtc agaatttaac atctattaag tctattactt actgttcaac      840

ctatgagcat acttttcctg atggtactgc catgaaggtt gcacgtactc caaagattaa      900

gaagactgtt gtcttgtctg agccacttgc tactatctac agggaaattg gttctccttt      960

tgtggataat gggagcgatg ctcgttctat cattaagaga ccagtgttcc tccacgcttt     1020

tgttaagtgt aagtgtggta gttatcattg gactgttggt gattggactt cctatgtctc     1080

cacttgctgt ggctttaagt gtaagccagt ccttgtggct tcatgctctg ctacgcctgg     1140

ttctgttgtg gttacgcgcg ctggtgctgg cactggtgtt aagtattaca acaacatgtt     1200

cctgcgccat gtggcagaca ttgatgggtt ggcattctgg cgaattctta aggtgcagtc     1260

caaagacgac ctcgcttgct ctggtaaatt ccttgaacac catgaggaag gtttcacaga     1320

tccttgctac tttttgaatg actcgagcat tgctactaag ctcaagtttg acatccttag     1380

tggcaagttt tctgatgaag tcaaacaagc tatctttgct ggtcatgttg ttgttggcag     1440

tgcgctcgtt gacattgttg acgatgcact gggacagcct tggtttatac gtaagcttgg     1500

tgaccttgca agtgcagctt gggagcagct taaggctgtc gttagaggcc ttaacctcct     1560

gtctgatgag gtcgtgctct ttggcaaaag acttagctgt gccactctta gtatcgttaa     1620

cggtgttttt gagtttatcg ccgaagtgcc agagaagttg gctgcggctg ttacagtttt     1680

tgtcaacttc ttgaatgagc tttttgagtc tgcctgtgac tgcttaaagg tcggaggtaa     1740

aacctttaac aaggttggct cctatgttct ttttgacaac gcattggtta agcttgtcaa     1800

ggcaaaagtt cgcggcccac gacaggcagg tgtttgtgaa gttcgttaca caagccttgt     1860

tattgggagt actaccaagg tggtttccaa gcgcgttgaa aatgccaatg tgaatctcgt     1920

cgtcgttgac gaggatgtga ccttcaacac cactggtcgt acagttgttg ttgacggact     1980

tgcattcttc gagagtgacg ggttttacag acatcttgct gatgctgacg ttgtcattga     2040

acatcctgtt tataagtctg cttgtgagct caagccagtt tttgagtgtg acccaatacc     2100

tgattttcct atgcctgtgg ccgctagtgt tgcagagctt tgtgtgcaaa ctgatctgtt     2160

gcttaaaaat tacaacactc cttataaaac ttacagctgc gttgtgagag gtgataagtg     2220

ttgtatcact tgcaccttac atttcacagc accaagttat atggaggctg ctgctaattt     2280

tgtagacctc tgtaccaaga acattggtac tgctggtttt catgagtttt acattatggc     2340

ccatgaacaa caggatcttc aagggttcgt aaccacttgt tgcacgatgt caggttttga     2400

gtgttttatg cctataatcc cacagtgtcc agcagtgctt gaagagattg atggtggtag     2460

catctggcgc tcttttatca ctggtcttaa tacaatgtgg gatttttgca agcatcttaa     2520

agtcagcttt ggactagatg gcattgttgt cactgtagca cgcaaattta aacgacttgg     2580

tgctctcttg gcagaaatgt ataacactta cctttcaact gtggtggaaa acttggtact     2640

ggccggtgtt agcttcaagt attatgccac cagtgtccca aaaattgttt tgggcggttg     2700

ttttcacagt gttaaaagcg ttctagcaag tgccttccag attcctgtcc aggcaggcgt     2760

tgagaagttt aaagtcttcc ttaactgtgt tcaccctgtt gtaccacgcg tcattgaaac     2820

ttcttttgtg gaattagaag agacgacatt taaaccacca gcactcaatg gtagtattgc     2880

tattgttgat ggctttgctt tctattatga tggaacacta tactatccca ccgatggtaa     2940

tagtgttgtg cctatctgtt ttaagaagaa gggtggtggt gatgtcaaat tctctgatga     3000

agtctctgtt agaaccattg acccagttta taaggtctcc cttgaatttg agttcgagtc     3060

tgagactatt atggctgtgc ttaataaggc tgttggtaat cgtatcaagg ttacaggtgg     3120

ttgggacgat gttgttgagt atatcaacgt tgccattgag gttcttaaag atcacatcga     3180

tgtgcctaag tactacatct atgatgagga aggtggcacc gatcctaatc tgcccgtaat     3240

ggtttctcag tggccgttga atgatgacac gatctcacag gatctgcttg atgttgaagt     3300

tgttactgat gcgccagttg atttcgaggg tgatgaagta gactcctctg accctgataa     3360

ggtggcagac gtggctaact ctgagcctga ggatgatggt cttaatgtag ctcctgaaac     3420

aaatgtagag tctgaagttg aggaagttgc cgcaaccttg tcctttatta aagatacacc     3480

ttccacagtt actaaagatc cttttgcttt tgactttgca agctatggag gacttaaggt     3540

tttaagacaa tctcataaca actgctgggt tacttctacc ttggtgcagc tacaattgct     3600

tggcatcgtt gatgaccctg caatggagct ttttagtgct ggtagagttg gtccaatggt     3660

tcgcaaatgc tatgagtcac aaaaggctat cttgggatct ttgggtgatg tgtcggcttg     3720

cctagagtct ctgactaagg acctacacac acttaagatt acctgttctg tagtctgtgg     3780

ttgtggtact ggtgaacgta tctatgaggg ttgtgctttt cgtatgacgc caactttgga     3840

accgttccca tatggtgctt gtgctcagtg tgctcaagtt ttgatgcaca cttttaaaag     3900

tattgttggc accggcatct tttgtcgaga tactactact ctctccttgg attctttggt     3960

tgtaaaacct ctttgtgcgg ctgcttttat aggcaaggat agtggtcatt atgtcactaa     4020

cttttatgat gctgctatgg ctattgatgg ttatggtcgt catcagataa agtatgacac     4080

actgaacact atttgtgtta aagacgttaa ttggacagca ccttttgtcc cagacgttga     4140

gcctgtattg gagcctgttg tcaaaccttt ctattcttat aagaatgttg atttttacca     4200

aggagatttt agtgaccttg ttaaacttcc atgtgatttt gttgttaatg ctgcaaatga     4260

gaatttgtct cacggtggcg gcatagcaaa ggccattgat gtttatacca agggcatgtt     4320

gcagaagtgc tcgaatgatt acattaaagc acacggtccc attaaagttg gacgtggtgt     4380

catgttggag gcattaggtc ttaaggtctt taatgttgtt ggtccacgta agggtaagca     4440

tgcacctgag cttcttgtta aggcttataa gtccgttttt gctaattcag gtgttgctct     4500

tacacctttg attagtgttg gaatttttag tgttcctttg gaagaatctt tatctgcttt     4560

tcttgcatgt gttggtgatc gccactgtaa gtgcttttgt tatagtgaca aagagcgcga     4620

ggcgatcatt aattacatgg atggcttggt agatgctatt ttcaaagatg cgcttgttga     4680

tactactcct gtccaggaag atgttcaaca agtttcacaa aaaccagttt tgcctaattt     4740

tgaacctttc aggattgaag gtgctcatgc tttctatgag tgcaaccctg aaggtttgat     4800

gtcattaggt gctgacaagc tggtgttgtt tacaaattcc aatttggatt tttgtagcgt     4860

tggtaagtgt cttaacaatg tgaccggcgg tgcattgctt gaagccataa atgtatttaa     4920

aaagagtaac aaaacagtgc ctgctggcaa ctgtgttact tttgagtgtg cagacatgat     4980

ttctattact attgtagtat tgccatctga tggtgatgct aattatgaca aaaattatgc     5040

acgcgccgtc gtcaaggtat ctaagcttaa aggcaagtta ttgcttgctg ttggtgatgc     5100

cacgttgtat tccaagttgt cccacctcag cgtggtaggt ttcgtatcca cacctgatga     5160

tgtggagcgt ttctacgcaa ataagagtgt ggttattaaa gttactgagg atacacgtag     5220

tgttaaggct gttaaagtag aatccactgt tacttatgga caacaaattg gaccttgtct     5280

tgttaatgac accgttgtca cagacaacaa acctgttgtt gctgatgttg tagctaaggt     5340

tgtaccaagt gctaattggg attcacatta tggttttgat aaggctggtg agttccacat     5400

gctagaccat actgggtttg cctttcctag tgaagttgtt aacggtaggc gtgtgcttaa     5460

aaccacagat aataactgtt gggttaatgt tacatgttta caattacagt ttgctagatt     5520

taggttcaag tcagcaggtc tacaggctat gtgggagtcc tattgtactg gtgatgttgc     5580

tatgtttgtg cattggttgt actggcttac tggtgttgac aaaggtcagc ctagtgattc     5640

agaaaatgca cttaacatgt tgtccaagta cattgtttct gctggttctg tcactattga     5700

acgtgtcacg catgacggct gttgttgtag taagcgtgtt gtcactgcac cagttgtgaa     5760

tgctagcgta ttgaagcttg gcgtcgagga tggtctttgt ccacatggtc ttaactacat     5820

tgacaaagtt gttgtagtca aaggtactac aattgttgtc aatgttggaa aacctgtagt     5880

ggcaccatca cacctctttc ttaagggtgt ttcttacaca acattcctag ataatggtaa     5940

cggtgttgtc ggccattata ctgtttttga tcatgacact ggtatggtgc atgatggaga     6000

tgcttttgta ccgggtgatc tcaatgtatc tcctgttaca aatgttgtcg tctcagagca     6060

gacggctgtt gtgattaaag accctgtgaa gaaagtagag ttagacgcta caaagctgtt     6120

agacactatg aattatgcat cggaaagatt cttttccttt ggtgatttta tgtcacgtaa     6180

tttaattaca gtgtttttgt acatccttag cattttgggt ctctgtttta gggcctttcg     6240

taagagggat gttaaagttc tagctggtgt accccaacgt actggtatta tattgcgtaa     6300

aagtgtgcgc tataatgcaa aggcgctggg tgtcttcttc aagctaaaac tttattggtt     6360

caaagttctt ggtaagttta gtttgggtat ttatgcattg tatgcattat tattcatgac     6420

aatacgcttt acacctatag gtggccctgt ttgtgatgat gttgttgctg gttatgctaa     6480

ttctagtttt gacaagaatg agtattgcaa cagtgttatt tgtaaggtct gtctctatgg     6540

gtaccaggaa ctttcggact tctctcacac acaggtagta tggcaacacc ttagagaccc     6600

attaattggt aatgtgatgc ctttctttta tttggcattt ctggcaattt ttgggggtgt     6660

ttatgtaaag gctattactc tctattttat tttccagtat cttaacattc ttggtgtgtt     6720

tttgggccta caacagtcca tttggttttt gcagcttgtg ccttttgatg tctttggtga     6780

cgagatcgtc gtctttttca tcgttacacg cgtattgatg ttccttaagc atgttttcct     6840

tggctgcgat aaggcatctt gtgtggcttg ctctaagagt gctcgcctta agcgcgttcc     6900

tgtccagact atttttcagg gtactagcaa atccttctac gtacatgcca atggtggttc     6960

taagttctgt aagaagcaca atttcttttg tttaaattgt gattcttatg gtccaggctg     7020

cacttttatt aatgacgtca ttgcaactga agttggtaat gttgtcaaac ttaatgtgca     7080

accgacaggt cctgccacta ttcttattga caaggttgaa ttcagtaatg gtttttacta     7140

tctttatagt ggtgacacat tttggaagta caactttgac ataacagata gcaaatacac     7200

ttgcaaagaa tcacttaaaa attgtagtat aatcacagac tttattgttt ttaacaataa     7260

tggttccaat gtaaatcagg ttaagaatgc atgtgtttat ttttcacaga tgctttgtaa     7320

acctgttaag ttagtggact cagcgttgtt ggccagtttg tctgttgatt ttggtgcaag     7380

cttacatagt gcttttgtta gtgtgttgtc gaatagtttt ggcaaagatc tgtcaagttg     7440

taatgacatg caggattgca agagcacatt gggttttgat gatgtaccat tggatacctt     7500

taatgctgct gttgctgagg ctcatcgtta cgatgtcctc ttgactgaca tgtcgttcaa     7560

caattttacc accagttatg caaaaccaga ggaaaaactt cccgtccatg acattgccac     7620

gtgtatgcgt gtaggtgcca agattgttaa tcataacgtt cttgtcaagg atagtatacc     7680

tgtggtgtgg cttgtacgtg atttcattgc cctttcggaa gaaactagga agtacattat     7740

tcgtacgact aaagttaagg gtataacctt catgttgacc tttaatgatt gtcgtatgca     7800

tactaccata cctactgttt gcattgcaaa taagaagggt gcaggtcttc ctagtttttc     7860

aaaggttaag aaattcttct ggtttttgtg tctgttcata gttgctgttt tctttgcact     7920

aagctttctt gattttagta ctcaggttag cagtgatagc gattatgact tcaagtatat     7980

tgagagtggc cagttgaaga cttttgacaa tccacttagt tgtgtgcata atgtctttag     8040

taacttcgac cagtggcatg aggccaagtt tggtttcacc cccgtcaaca atcctagttg     8100

tcctatagtc gttggtgtat cagacgaagc gcgcactgtt ccaggtatcc cagcaggtgt     8160

ttatttagct ggtaaaacac ttgtttttgc tattaacacc atttttggta catctggttt     8220

gtgctttgat gctagtggcg ttgctgataa gggcgcttgc atttttaatt cggcttgcac     8280

cacattatct ggtttgggtg gaactgctgt ctactgttat aagaatggtc tagttgaagg     8340

tgctaaactt tatagtgagt tggcacctca tagctactat aaaatggtag atggtaatgc     8400

tgtgtcttta cctgaaatta tctcacgcgg ctttggcatc cgtactatcc gtacaaaggc     8460

tatgacctac tgtcgcgttg gccagtgtgt gcaatctgca gaaggtgttt gttttggcgc     8520

cgatagattc tttgtctata atgcagaatc tggttctgac tttgtttgtg gcacagggct     8580

ctttacattg ttgatgaacg ttattagtgt tttttccaag acagtaccag taactgtgtt     8640

gtctggtcaa atacttttta attgcattat tgcttttgct gctgttgcgg tgtgtttctt     8700

atttacaaag tttaagcgca tgttcggtga tatgtctgtt ggcgttttca ctgtcggtgc     8760

ttgtactttg ttgaacaatg tttcctacat tgtaacacag aacacacttg gcatgttggg     8820

ctatgcaact ttgtactttt tgtgcactaa aggtgttaga tatatgtgga tttggcattt     8880

gggatttttg atctcatata tacttattgc accatggtgg gttttgatgg tttatgcctt     8940

ttcagccatt tttgagttta tgcctaacct ttttaagctt aaggtttcaa cacaactttt     9000

tgagggtgac aagttcgtag gctcttttga aaatgctgca gcaggtacat ttgtgcttga     9060

tatgcatgcc tatgagagac ttgccaactc tatctcaact gcaaaactgc gtcagtatgc     9120

tagtacttac aataagtaca agtattattc aggcagtgct tcagaggctg attacaggct     9180

tgcttgtttt gcccatttgg ccaaggctat gatggattat gcttctaatc acaacgacac     9240

gttatacaca ccacccactg tgagttacaa ttcaactcta caggctggct tgcgtaagat     9300

ggcacaacca tctggtgttg ttgagaagtg catagttcgt gtttgctatg gtaatatggc     9360

tcttaatggc ctatggcttg gtgatactgt tatctgccca cgccatgtta tagcgtctag     9420

tactactagc actatagatt atgactatgc cctttctgtt ttacgcctcc acaacttctc     9480

catttcatct ggtaatgttt tcctaggtgt tgtgggtgta accatgcgag gtgctttgtt     9540

gcagataaag gttaatcaaa acaatgtcca cacgcctaag tacacctatc gcacagttag     9600

accgggtgaa tcttttaata tcttggcgtg ctatgatggt tctgcagctg gtgtttacgg     9660

cgttaacatg cgctctaatt acactattag aggctcgttc attaatggcg cttgtggttc     9720

acctggttat aacattaaca atggtaccgt tgagttttgc tatttacacc agcttgaact     9780

tggttcaggc tgtcatgttg gtagcgactt agatggtgtt atgtatggtg gttatgagga     9840

ccaacctact ttgcaagttg aaggcgctag tagtctgttt acagagaatg tgttggcatt     9900

tctttatgca gcactcatta atggttctac ctggtggctt agttcttcta ggattgctgt     9960

agacaggttt aatgagtggg ctgttcataa tggtatgaca acagtagtta atactgattg    10020

cttttctatt cttgctgcta agactggtgt tgatgtacaa cgtttgttgg cctcaatcca    10080

gtctctgcat aagaattttg gtggaaagca aattcttggc tatacctcgt tgacagatga    10140

gtttactaca ggtgaagtta tacgtcaaat gtatggcgtt aatcttcaga gtggttatgt    10200

ttcacgcgcc tgtagaaatg tcttgctggt tggttctttt ctgactttct tttggtcaga    10260

attagtttcc tacactaagt tcttttgggt aaatcctggt tatgtcacac ctatgtttgc    10320

gtgtttgtca ttgctgtcct cacttttgat gttcacactc aagcataaga cattgttttt    10380

ccaggtcttt ctaatacctg ctctgattgt tacatcttgc attaatttgg catttgatgt    10440

tgaagtctac aactatttgg cagagcattt tgattaccat gtttctctca tgggttttaa    10500

tgcacaaggt cttgttaaca tctttgtctg ctttgttgtt atcattttac acggcacata    10560

cacatggcgc ttttttaaca cacctgtgag ttctgtcact tatgtggtag ctttgctgac    10620

tgcggcatat aactattttt acgctagtga cattcttagt tgtgctatga cactatttgc    10680

tagtgtgact ggcaactggt tcgttggtgc tgtttgttat aaagctgctg tttatatggc    10740

cttgagattt cctacttttg tggctatttt tggtgatatt aagagtgtta tgttctgtta    10800

ccttgtgttg ggttatttta cctgttgctt ctacggtatt ctctactggt tcaacaggtt    10860

ctttaaggtt agtgtaggtg tctatgacta tactgttagt gctgctgagt ttaagtatat    10920

ggttgctaac ggcctacgtg caccaactgg aacacttgat tcactacttc tgtctgccaa    10980

attgattggt attggtggtg agcggaatat taagatttct tccgttcagt ctaaactgac    11040

tgatattaag tgtagtaacg ttgtgctttt aggctgtctc tctagcatga atgtctcagc    11100

aaattcaaca gaatgggcct attgtgttga cttgcataac aagatcaacc tgtgtaatga    11160

cccagaaaaa gcgcaggaaa tgctacttgc tttgttggca tttttcctta gtaagaatag    11220

tgcttttggt ttagatgact tattggaatc ctattttaat gacaatagta tgttgcagag    11280

tgttgcatct acttatgtcg gtttgccttc ttatgtcatt tatgaaaatg cacgccaaca    11340

gtatgaagat gctgttaata atggttctcc acctcagttg gttaagcaat tgcgccatgc    11400

tatgaatgtg gcaaagagtg aatttgaccg tgaggcttct actcagcgta agcttgatag    11460

aatggcggag caggctgcag cacagatgta caaagaggca agagcagtta ataggaagtc    11520

caaagttgta agtgctatgc attcactgct ttttggtatg ttgagacgtt tggatatgtc    11580

ttctgtagac actattctca acttggcaaa ggatggggtt gtacctctgt ctgtcatacc    11640

ggcagtcagt gctactaagc ttaacattgt tacttctgat atcgattctt ataatcgtat    11700

ccagcgtgag ggatgtgtcc actacgctgg taccatttgg aatataattg atatcaagga    11760

caatgatggc aaggtggtac acgttaagga ggtaaccgca cagaatgctg agtccctgtc    11820

atggcccctg gtccttgggt gtgagcgtat tgtcaagctc cagaataatg aaattattcc    11880

tggtaagctg aagcagcgct ccattaaggc agaaggagat ggcatagttg gagaaggtaa    11940

ggcactttac aataatgagg gtggacgtac ttttatgtat gctttcatct cggacaaacc    12000

ggacctgcgt gtagttaagt gggagttcga tggtggttgt aacactattg agctagaacc    12060

accacgtaag ttcttggtgg attctcctaa tggtgcacag atcaagtatc tctactttgt    12120

tcgtaacctt aacacgttac gtaggggtgc cgttctcggc tacataggtg ccactgtacg    12180

cttgcaggct ggtaaacaaa cagaacaggc tattaactct tcattgttga cactttgcgc    12240

tttcgctgtg gatcctgcta agacctacat cgatgctgtc aaaagtggtc acaaaccagt    12300

aggtaactgt gttaagatgt tggccaatgg ttctggtaat ggacaagctg ttactaatgg    12360

tgtggaggct agtactaacc aggattcata cggtggtgcg tccgtgtgtc tatattgtag    12420

agcacatgtt gagcatccat ctatggatgg tttttgcaga ctgaaaggca agtacgtaca    12480

ggttccacta ggtacagtgg atcctatacg ttttgtactt gagaatgacg tttgcaaggt    12540

ttgtggttgt tggctggcta atggctgcac ttgtgacaga tccattatgc aaagtactga    12600

tatggcttat ttaaacgagt acggggctct agtgcagctc gactagagcc ctgtaacggt    12660

actgatacac aacatgtgta tcgtgctttt gacatctaca acaaggatgt tgcttgtcta    12720

ggtaaattcc tcaaggtgaa ctgtgttcgc ctgaagaatt tggataagca tgatgcattc    12780

tatgttgtca aaagatgtac caagtctgcg atggaacacg agcaatccat ctatagcaga    12840

cttgaaaagt gtggagccgt agccgaacac gatttcttca cttggaagga tggtcgtgca    12900

atctatggta acgtttgtag aaaggatctt accgagtata ctatgatgga tttgtgttac    12960

gctttacgta actttgatga aaacaattgc gatgttctta agagcatttt aattaaggta    13020

ggtgcttgtg aggagtccta cttcaataat aaagtctggt ttgaccctgt tgaaaatgaa    13080

gacattcatc gtgtctatgc agtgttaggt accattgttt cacgtgctat gcttaaatgc    13140

gttaagttct gtgatgcaat ggttgaacaa ggtatagttg gtgttgtcac attagataat    13200

caggatctta atggtgattt ttatgatttt ggtgatttta cttgtagcat caagggaatg    13260

ggtataccca tttgcacatc atattactct tatatgatgc ctgttatggg tatgactaat    13320

tgccttgcta gtgagtgttt tgttaagagt gatatatttg gtgaggattt caagtcatat    13380

gacctgctgg aatatgattt cacggagcat aagacagcac tcttcaacaa gtatttcaag    13440

tattggggac tgcaatacca ccctaactgt gtggactgca gtgatgagca gtgcatagtt    13500

cactgtgcca acttcaatac gttgttttcc actactatac ctattacggc atttggacct    13560

ttgtgtcgca agtgctggat cgatggtgtt ccactggtaa ctacagctgg ttatcatttt    13620

aaacagttag gtatagtttg gaataatgac cttaacttac actctagcag gctctctatt    13680

aacgaactac tccagttttg tagtgatcct gcactgctta tagcatcatc accagccctt    13740

gttgatcagc gtactgtttg cttttcagtt gcagcgctag gtacaggtat gactaaccag    13800

actgtgaaac ctggccattt caataaggag ttttatgact tcttacttga gcaaggtttc    13860

ttctctgagg gttctgagct tactttaaag cacttcttct ttgcacagaa gggtgatgca    13920

gctgttaagg attttgacta ctataggtat aatagaccca ctgttctgga catttgccaa    13980

gctcgcgtcg tgtatcaaat agtgcaacgc tattttgata tttacgaagg tggttgtatc    14040

actgctaaag aagtggttgt cacaaacctt aacaagagcg caggttatcc tttgaacaag    14100

tttggtaaag ctggtcttta ctatgagtct ttatcctatg aggaacagga tgaactttat    14160

gcttatacta agcgtaacat cctgcccact atgacacagc tcaaccttaa atatgctata    14220

agtggcaaag aacgtgcacg cacagtgggt ggtgtttcgc tcttgtcaac catgactact    14280

cggcagtatc atcagaaaca ccttaagtcc atagttaata ctaggggcgc ttcggttgtt    14340

attggtacta ctaagtttta tggtggttgg gacaatatgc ttaagaacct tattgatggt    14400

gttgaaaatc cgtgtcttat gggttgggat tacccaaagt gcgacagagc actgcccaat    14460

atgatacgca tgatttcagc catgatttta ggctctaagc acaccacatg ctgcagttcc    14520

actgaccgct ttttcaggtt gtgcaatgaa ttggctcaag tccttactga ggttgtttat    14580

tctaatggag gtttttattt gaagccaggt ggtactacct ctggtgatgc aaccaccgca    14640

tatgcaaact cagttttcaa tatcttccaa gcagtaagtg ccaatgttaa caaacttctt    14700

agtgttgaca gcaatgtctg tcataattta gaagttaagc aattgcagcg taagctttat    14760

gagtgctgtt atagatcaac taccgtcgat gaccagttcg tcgttgagta ttatggttac    14820

ttgcgtaaac atttttcaat gatgattctt tctgatgatg gcgttgtttg ttataacaat    14880

gactatgcat cacttggtta tgtcgctgat cttaacgcat tcaaggctgt tttgtattac    14940

cagaacaatg tcttcatgag cgcctctaaa tgttggatcg agcctgacat taataaaggt    15000

cctcatgaat tttgctcgca gcatactata cagattgtcg ataaagatgg tacttattac    15060

cttccttacc ctgatccttc aagaatcctc tctgcaggtg tgtttgttga tgacgttgtt    15120

aaaactgatg cggttgtatt gcttgaacgt tatgtgtcat tggctataga tgcctacccg    15180

ttatctaagc atgaaaaccc tgaatataag aaggtgtttt atgtgctttt ggattgggtt    15240

aagcatctgt acaaaacttt gaatgctggt gtgttagagt ctttttctgt cacacttctg    15300

gaagattcta ctgctaaatt ctgggatgag agcttttatg ccaacatgta tgagaaatct    15360

gcagttttac aatctgcagg gctttgtgtt gtttgtggct ctcaaactgt tttacgttgt    15420

ggtgattgtc tacggcgtcc tatgctttgt actaagtgtg cttatgatca tgtcattgga    15480

acaactcaca agttcatttt ggccatcact ccatatgtgt gttgtgcttc agattgtggt    15540

gtcaatgatg taactaagct ctacttaggt ggtcttagtt attggtgtca tgaacacaag    15600

ccacgtcttg cattcccgtt gtgctctgct ggtaatgttt ttggcttgta caaaaattct    15660

gctaccggct cacccgatgt tgaagacttt aatcgcattg ctacatccga ttggactgat    15720

gtttctgact acaggttggc aaatgatgtc aaggactcat tgcgtctgtt tgcagcggaa    15780

actatcaagg ccaaggagga gagcgttaag tcatcctacg cttgtgcaac actacatgag    15840

gttgtaggac ctaaagagtt gttgctcaaa tgggaagtcg gcagacccaa accacccctt    15900

aatagaaatt cggttttcac ttgttatcat ataacgaaga acaccaaatt tcaaatcggt    15960

gagtttgtgt ttgagaaggc agaatatgat aatgatgctg taacatataa aactaccgcc    16020

acaacaaaac ttgttcctgg catggttttt gtgcttacct cacataatgt tcagccattg    16080

cgcgcaccga ccattgctaa tcaagaacgt tattccacta tacataagtt gcatcctgct    16140

tttaacatac ctgaagctta ttctagctta gtgccctatt accaattgat tggtaagcag    16200

aagattacaa ctattcaggg acctcccggt agtggtaaat ctcactgtgt tatagggcta    16260

ggtttgtact atccaggtgc acgtatagtg tttacagctt gttctcatgc agcggtcgat    16320

tcactttgtg tgaaagcctc cactgcttat agcaatgaca aatgttcacg catcatacca    16380

cagcgcgctc gtgttgagtg ttatgatggt ttcaagtcta ataatactag tgctcagtac    16440

cttttctcta ctgtcaatgc tttgccagag tgcaatgcgg acattgttgt ggtggatgag    16500

gtctctatgt gcactaatta tgacttgtct gtcataaatc agcgcatcag ctataggcat    16560

gtagtctatg ttggtgaccc tcaacagctg cctgcaccac gtgttatgat ttcacgtggt    16620

actttggaac caaaggacta caacgttgtc actcaacgca tgtgtgccct taagcctgat    16680

gttttcttgc acaagtgtta tcgctgtcct gctgagatag tgcgtactgt gtctgagatg    16740

gtctatgaaa accaattcat tcctgtgcac ccagatagca agcagtgttt taaaatcttt    16800

tgcaagggta atgttcaggt tgataatggt tcaagcatta atcgcaggca attggatgtt    16860

gtgcgtatgt ttttggctaa aaatcctagg tggtcaaagg ctgtttttat ttctccttat    16920

aacagccaga attatgttgc cagccgcatg ctaggtttac aaattcagac agttgactca    16980

tcccagggta gtgagtatga ctatgtcatt tacacacaaa cttcagatac tgcccatgcc    17040

tgtaatgtta acaggtttaa tgttgccatc acaagggcca agaaaggcat attatgtata    17100

atgtgcgata ggtccctttt tgatgtgctt aaattctttg agcttaaatt gtctgatttg    17160

caggctaatg agggttgtgg tctttttaaa gactgtagca gaggtgatga tctgttgcca    17220

ccatctcacg ctaacacctt catgtcttta gcggacaatt ttaagactga tcaagatctt    17280

gctgttcaaa taggtgttaa tggacccatt aaatatgagc atgttatctc gtttatgggt    17340

ttccgttttg atatcaacat acccaaccat catactctct tttgcacacg cgactttgcc    17400

atgcgcaatg ttagaggttg gttgggtttt gacgttgaag gagcacatgt tgttggctct    17460

aacgtcggta caaatgtccc attgcaatta gggttttcta acggtgttga ttttgttgtc    17520

agacctgaag gttgcgttgt aactgagtct ggtgactaca ttaaacccgt cagagctcgt    17580

gctccaccag gggaacaatt cgcacacctt ttgcctctac ttaaacgcgg ccaaccatgg    17640

gatgttgttc gcaaacgtat agtgcagatg tgtagtgact acctggccaa cctatcagac    17700

atactaattt ttgtgttgtg ggctggtggt ttggagttga caactatgcg ttattttgtc    17760

aagattggac caagtaagag ttgtgattgt ggtaaggttg ctacttgtta caatagtgcg    17820

ctgcatacgt actgttgttt caaacatgcc cttggttgtg attatctgta taacccatac    17880

tgtattgata tacagcagtg gggatacaag ggatcactta gccttaacca ccatgagcat    17940

tgtaatgtac atagaaacga gcacgtggct tctggtgatg ccataatgac tcgttgtctg    18000

gccatacatg attgctttgt caagaacgtt gactggtcca tcacataccc atttattggt    18060

aatgaggctg ttattaataa gagcggccga attgtgcaat cacataccat gcggtcagtt    18120

cttaagttat acaatccgaa agccatatat gatattggca atcctaaggg catcagatgt    18180

gcagtaacgg atgctaagtg gttttgcttt gacaagaatc ctactaattc taatgtcaag    18240

acattggagt atgactatat aacacatggc caatttgatg ggttgtgctt gttttggaat    18300

tgtaatgtgg acatgtatcc agaattttct gtggtctgtc gttttgacac tcgctgtagg    18360

tcaccactca acttggaggg ttgtaatggt ggttcactgt atgttaataa tcatgcattc    18420

catacaccgg cttttgacaa gcgtgctttt gctaagttga agccaatgcc atttttcttt    18480

tatgatgata ctgagtgtga caagttacag gactccataa actatgttcc tcttagggct    18540

agtaactgca ttactaaatg taatgttggt ggagctgtct gtagtaagca ttgtgctatg    18600

tatcatagct atgttaatgc ttacaacact tttacgtcgg cgggctttac tatttgggtg    18660

cctacttcgt ttgacaccta taatctgtgg cagacattta gtaacaattt gcaaggtctt    18720

gagaacattg ctttcaatgt cgtaaagaaa ggatcttttg ttggtgccga aggtgaactt    18780

cctgtagctg tggttaatga caaagtgctc gttagagatg gtactgttga tactcttgtt    18840

tttacaaaca agacatcact acccactaac gtagcttttg agttgtatgc caagcgtaag    18900

gtaggactca ccccacccat tacgatccta cgtaacttgg gtgtagtttg tacatctaag    18960

tgtgtcattt gggactatga agccgaacgt ccacttacta cttttacaaa ggatgtttgt    19020

aaatataccg actttgaggg tgacgtctgt acactctttg ataacagcat tgttggttca    19080

ttagagcgat tctctatgac ccaaaatgct gtgcttatgt cacttacagc cgttaaaaag    19140

cttactggca taaagttaac ttatggttat cttaatggtg tcccagttaa cacacatgaa    19200

gataaacctt ttacttggta tatttacact aggaagaacg gcaaattcga ggactatcct    19260

gatggctatt ttacccaagg tagaacaacc gctgatttta gccctcgtag cgacatggaa    19320

aaggacttcc taagtatgga tatgggtctg tttattaaca agtacggact tgaagattac    19380

ggctttgagc acgttgtgta tggtgatgtt tcaaaaacca cccttggtgg tttacatcta    19440

ctaatttcgc aggtgcgtct gtcctgtatg ggtgtgctta aaatagacga gtttgtgtct    19500

agtaatgata gcacgttaaa gtcctgtact gttacatatg ctgataaccc tagtagtaag    19560

atggtttgca cgtatatgga tctcctgctt gacgattttg tcagcattct taaatcgttg    19620

gatttgggcg ttgtatctaa agttcatgaa gttatggtcg attgtaaaat gtggaggtgg    19680

atgttgtggt gtaaggatca taaactccag acattttatc cgcaacttca ggccagtgaa    19740

tggaagtgtg gttattccat gccttctatt tacaagatac aacgtatgtg tttagaacct    19800

tgcaatctct acaactatgg tgctggtatt aagttacctg atggcattat gtttaacgta    19860

gttaaataca cacagctttg tcaatatctc aatagcacca caatgtgtgt accccatcac    19920

atgcgtgtgc tacatcttgg tgctggctcc gacaagggtg ttgcacctgg cacggctgtc    19980

ttacgacgtt ggttgccact ggatgccatt atagttgaca atgatagtgt ggattacgtt    20040

agcgatgctg attatagtgt tacaggagat tgctctacct tatacctgtc agataagttt    20100

gatttagtta tatctgatat gtatgatggt aagattaaaa gttgtgatgg ggagaacgtg    20160

tctaaagaag gcttctttcc ctatattaat ggtgtcatca ccgaaaagtt ggcacttggt    20220

ggtactgtag ctattaaggt gacggagttt agttggaata agaagttgta tgaactcatt    20280

cagaagtttg agtattggac aatgttctgt accagtgtta acacgtcatc gtcagaggca    20340

tttttaattg gtgttcacta tttaggtgat tttgcaagtg gcgctgtgat tgacggcaac    20400

actatgcatg ccaattatat cttctggcgt aattccacaa ttatgactat gtcttacaat    20460

agtgtacttg atttaagcaa gttcaattgt aagcataagg ctacagttgt cattaattta    20520

aaagattcat ccattagtga tgttgtgtta ggtttgttga agaatggtaa gttgctagtg    20580

cgtaataatg acgccatttg tggtttttct aatcatttgg tcaacgtaaa caaatgaagt    20640

ctttaaccta cttctggttg ttcttaccag tactttcaac acttagccta ccacaagatg    20700

tcaccaggtg ctcagctaac actaatttta ggcggttctt ttcaaaattt aatgttcagg    20760

cgcctgcagt tgttgtactg ggcggttatc tacctattgg tgaaaaccag ggtgtcaatt    20820

caacttggta ctgtgctggc caacatccaa ctgctagtgg cgttcatggt atctttgtta    20880

gccatattag aggtggtcat ggctttgaga ttggcatttc gcaagagcct tttgacccta    20940

gtggttacca gctttattta cataaggcta ctaacggtaa cactaatgct actgcgcgac    21000

tgcgcatttg ccagtttcct agcattaaaa cattgggccc cactgctaat aatgatgtta    21060

caacaggtcg taattgccta tttaacaaag ccatcccagc gcatatgagt gaacatagtg    21120

ttgtcggcat aacatgggat aatgatcgtg tcactgtctt ttctgacaag atctattatt    21180

tttattttaa aaatgattgg tcccgtgttg cgacaaagtg ttacaacagt ggaggttgtg    21240

ctatgcaata tgtttacgaa cccacctatt acatgcttaa tgttactagt gctggtgagg    21300

atggtatttc ttatcaaccc tgtacagcta attgcattgg ttatgctgcc aatgtatttg    21360

ctactgagcc caatggccac ataccagaag gttttagttt taataattgg tttcttttgt    21420

ccaatgattc cactttggtg catggtaagg tggtttccaa ccaaccattg ttggtcaatt    21480

gtcttttggc cattcctaag atttatggac taggccaatt tttctccttt aatcaaacga    21540

tcgatggtgt ttgtaatgga gctgctgtgc agcgtgcacc agaggctctg aggtttaata    21600

ttaatgacat ctctgtcatt cttgctgaag gctcaattgt acttcatact gctttaggaa    21660

caaatttttc ttttgtttgc agtaattcct cagatcctca cttagccacc ttcgccatac    21720

ctctgggtgc tatccaagta ccctattatt gttttcttaa agtggatact tacaactcca    21780

ctgtttataa attcttggct gttttacctc ctaccgtcag ggaaattgtc atcaccaagt    21840

atggtgatgt ttatgtcaat gggtttggct atttgcatct cggtttgttg gacgctgtca    21900

caattaattt cactggtcat ggcactgacg atgacgtttc aggtttctgg accatagcat    21960

cgactaattt tgttgatgca cttatcgaag ttcaaggaac tgccattcag cgtattcttt    22020

attgtgatga tcctgttagc caactcaagt gttctcaggt tgcttttgac cttgacgatg    22080

gtttctaccc tatttcttct agaaaccttc tgagtcatga acagccaatt tcttttgtta    22140

ctttgccatc atttaatgat cattcttttg ttaacattac tgtctctgcg tcctttggtg    22200

gtcatagtgg tgccaacctt attgcatctg acactactat caatgggttt agttctttct    22260

gtgttgacac tagacaattt accatttcac tgttttataa cgttacaaac agttatggtt    22320

atgtgtctaa atcacaggac agtaattgcc ctttcacctt gcaatctgtt aatgattacc    22380

tgtcttttag taaattttgt gtttccacca gccttttggc tagtgcctgt accatagatc    22440

tttttggtta ccctgagttt ggtagtggtg ttaagtttac gtccctttac tttcaattca    22500

caaagggtga gttgattact ggcacgccta aaccacttga aggtgtcacg gacgtttctt    22560

ttatgactct ggatgtgtgt accgagtata ctatctatgg ctttaaaggt gagggtatca    22620

ttacccttac aaattctagc tttttggcag gtgtttatta cacatctgat tctggacagt    22680

tgttagcctt taagaatgtc actagtggtg ctgtttattc tgttacgcca tgttcttttt    22740

cagagcaggc tgcatatgtt gatgatgata tagtgggtgt tatttctagt ttgtctagct    22800

ccacttttaa cagtactagg gagttgcctg gtttcttcta ccattctaat gatggctcta    22860

attgtacaga gcctgtgttg gtgtatagta acataggcgt ttgtaaatct ggcagtattg    22920

gctacgtccc atctcagtct ggccaagtca agattgcacc cacggttact gggaatatta    22980

gtattcccac caactttagt atgagtatta ggacagaata tttacagctt tacaacacgc    23040

ctgttagtgt tgattgtgcc acatatgttt gtaatggtaa ctctcgttgt aaacaattac    23100

tcacccagta cactgcagca tgtaagacca tagagtcagc attacaactc agcgctaggc    23160

ttgagtctgt tgaagttaac tctatgctta ctatttctga agaggctcta cagttagcta    23220

ccatcagttc gtttaatggt gatggatata attttactaa tgtgctgggt gtttctgtgt    23280

atgatcctgc aagtggcagg gtggtacaaa aaaggtcttt tattgaagac ctgcttttta    23340

ataaagtggt tactaatggc cttggtactg ttgatgaaga ctataagcgc tgttctaatg    23400

gtcgctctgt ggcagatcta gtctgtgcac agtattactc tggtgtcatg gtactacctg    23460

gtgttgttga cgctgagaag cttcacatgt atagtgcgtc tctcatcggt ggtatggtgc    23520

taggaggttt tacttctgca gcggcattgc cttttagcta tgctgttcaa gctagactca    23580

attatcttgc tctacagacg gatgttctac agcggaacca gcaattgctt gctgagtctt    23640

ttaactctgc tattggtaat ataacttcag cctttgagag tgttaaagag gctattagtc    23700

aaacttccaa gggtttgaac actgtggctc atgcgcttac taaggttcaa gaggttgtta    23760

actcgcaggg tgcagctttg actcaactta ccgtacagct gcaacacaac ttccaagcca    23820

tttctagttc tattgatgac atttactctc gactggacat tctttcagcc gatgttcagg    23880

ttgaccgtct catcaccggc agattatcag cacttaatgc ttttgttgct caaaccctca    23940

ctaagtatac tgaggttcag gctagcagga agctagcaca gcaaaaggtt aatgagtgcg    24000

ttaaatcgca atctcagcgt tatggttttt gtggtggtga tggcgagcac attttctctc    24060

tggtacaggc agcacctcag ggcctgctgt ttttacatac agtacttgta ccgggtgact    24120

ttgtagatgt tattgccatc gctggcttat gcgttaacga tgaaattgcc ttgactctac    24180

gtgagcctgg cttagtcttg tttacgcatg aacttcaaaa tcatactgcg acggaatatt    24240

ttgtttcatc gcgacgtatg tttgaaccta gaaaacctac cgttagtgat tttgttcaaa    24300

ttgagagttg tgtggtcacc tatgtcaatt tgactagaga ccaactacca gatgtaatcc    24360

cagattacat cgatgttaac aaaacacttg atgagatttt agcttctctg cccaatagaa    24420

ctggtccaag tcttccttta gatgttttta atgccactta tcttaagctc actggtgaaa    24480

ttgcagattt agagcagcgt tcagagtctc tccgtaatac tacagaggag ctccaaagtc    24540

ttatatataa tatcaacaac acactagttg accttgagtg gctcaaccga gttgagacat    24600

atatcaagtg gccgtggtgg gtttggttga ttattttcat tgttctcatc tttgttgtgt    24660

cattactagt gttctgctgc atttccactg gttgttgtgg atgctgcggc tgctgctgtg    24720

cttgtttttc aggttgttgt aggggtccta gacttcaacc tttggcgcta taaaaatgcg    24780

ctctttatta tttttaatac tacgacactt tctttcctca atggtaaagc agcttattat    24840

gacggcaaat ccattgtgat tctagaaggt ggtgaccatt acatcacttt tggcaactct    24900

tttgttgctt tcgttagtag cattgacttg tatctagcta tacgtgggcg gcaagaagcc    24960

gacctacagc tgttgcgaac tgttgagctt cttgatggca agaagcttta tgtcttttcg    25020

caacatcaaa ttctcaattc aactagacga gtatgctaca attagtgaat gataatggtc    25080

tagtagttaa tgttatactt tggcttttcg tactcttttt cctgcttatt ataagcatta    25140

ctttcgtcca attggttaat ctgtgcttca cttgtcaccg gttgtgtaat agcgcagttt    25200

acacacctat agggcgtttg tatagagttt ataagtctta catgcaaata gaccccctcc    25260

ccagtactgt tattgacgta taaacgaaat atgtctaacg gttctattcc cgttgatgag    25320

gtgattcaac accttagaaa ctggaatttc acatggaata tcatactgac gatactactt    25380

gtagtgcttc agtatggcca ttacaagtac tctgcgttct tgtatggtgt caagatggct    25440

attctatgga tactttggcc tcttgtgtta gcactgtcac tttttgatgc atgggctagc    25500

tttcaggtca attgggtctt ttttgctttc agcatcctta tggcttgcat cactcttatg    25560

ctgtggataa tgtactttgt caatagcatt cggttgtggc gcaggacaca ttcttggtgg    25620

tctttcaatc ctgaaacaga cgcgcttctc actacttctg tgatgggccg acaggtctgc    25680

attccagtgc ttggagcacc aactggtgta acgctaacac tccttagtgg tacattgctt    25740

gtagagggct ataaggttgc tactggcgta caggtaagtc aattacctaa tttcgtcaca    25800

gtcgccaagg ccactacaac aattgtctac ggacgtgttg gtcgttcagt caatgcttca    25860

tctggcactg gttgggcttt ctatgtccgg tccaaacacg gcgactactc agctgtgagt    25920

aatccgagtt cggttctcac agatagtgag aaagtgcttc atttagtcta aacagaaact    25980

ttatggcttc tgtcagtttt caggatcgtg gccgcaaacg ggtgccatta tccctctatg    26040

cccctcttag ggttactaat gacaaacccc tttctaaggt acttgcaaat aatgctgtac    26100

ccactaataa aggaaataag gaccagcaaa ttggatactg gaatgagcaa attcgctggc    26160

gcatgcgccg tggtgagcga attgaacaac cttccaattg gcatttctac tacctcggaa    26220

caggacctca cgccgacctc cgctatagga ctcgtactga gggtgttttc tgggttgcta    26280

aagaaggcgc aaagactgaa cccactaacc tgggtgtcag aaaggcgtct gaaaagccta    26340

tcattccaaa tttctctcaa cagcttccca gcgtagttga gattgttgaa cctaacacac    26400

ctcctacttc acgtgcaaat tcacgtagca ggagtcgtgg taatggcaac aacaggtcca    26460

gatctccaag taacaacaga ggcaacaacc agtcccgcgg taattcacag aatcgtggaa    26520

ataaccaggg tcgtggagct tctcagaaca gaggaggcaa taataataac aataacaagt    26580

ctcgtaacca gtccaagaac agaaaccagt caaatgaccg tggtggtgta acatcacgcg    26640

atgatctggt ggctgctgtc aaggatgccc ttaaatcttt gggtattggc gaaaaccctg    26700

acaagcttaa gcaacagcag aagcccaaac aggaaaggtc tgacagcagc ggcaaaaata    26760

cacctaagaa gaacaaatcc agagccactt cgaaagaacg tgacctcaaa gacatcccag    26820

agtggaggag aattcccaag ggcgaaaata gcgtagcagc ttgcttcgga cccaggggag    26880

gcttcaaaaa ttttggagat gcggactttg tcgaaaaagg tgttgatgcc tcaggctatg    26940

ctcagatcgc cagtttagca ccaaatgttg cagcattgct ctttggtggt aatgtggctg    27000

ttcgtgagct agcggactct tacgagatta catataatta taaaatgact gtgccaaagt    27060

ctgatccaaa tgtagagctt cttgtttcac aggtggatgc atttaaaact gggaatgcaa    27120

aaccccagag aaagaaggaa aagaagaaca agcgtgaaac cacgcagcag ctgaatgaag    27180

aggccatcta cgatgatgtg ggtgtgccat ctgatgtgac tcatgcctat ttggaatggg    27240

acacagctgt tgatggtggt gacacggccg ttgaaattat caacgagatc ttcgacacag    27300

gaaattaaac aatgtttgac tggcttaccc tggctatgtc ccagggtagt gccattacac    27360

tgttattact gagtgttttt ctagcgactt ggctgctggg ctatggcttt gccctctaac    27420

tagcggtctt ggtcttgcac acaacggtaa gccagtggta atgtcagtgc aagaaggata    27480

ttaccatagc actgtcatga ggggaacgca gtaccttttc atctaaacct ttgcacgagt    27540

aatcaaagat ccgcttgacg agcctatatg gaagagcgtg ccaggtattt gacttaagga    27600

ctgttagtaa ctgaagacct gacggtgttg atatggatac ac                       27642


<210>  11
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  11
tcattactag tgttctgctg catttc                                            26


<210>  12
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  12
cacagattaa ccaattggac gaaggt                                            26


