                         SEQUENCE LISTING

<110>  Albert Einstein College of Medicine
 
<120>  ATTB CELL LINE, TRANSGENIC CELL LINES DERIVED 
       THEREFROM, AND METHODS OF MAKING THE SAME

<130>  XVS0010PCT

<150>  US 63/082,701
<151>  2020-09-28

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  118
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Vero AAVS1 sequence

<400>  1
tctcacaggg aaaactgatg cacagaggaa caatacaaat ggcttgtcga cgacggcggt       60

ctccgtcgtc aggatcattc ggggctagaa aggtgaagac ccaaaattag aactcagg        118


<210>  2
<211>  38
<212>  DNA
<213>  Mycobacterium smegmatis attB

<400>  2
ggcttgtcga cgacggcggt ctccgtcgtc aggatcat                               38


<210>  3
<211>  48
<212>  DNA
<213>  Mycobacteriophage Bxb1 attP

<400>  3
ggtttgtctg gtcaaccacc gcggtctcag tggtgtacgg tacaaacc                    48


<210>  4
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer

<400>  4
aactcggaaa ctgccatagc agg                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer

<400>  5
gtttcttagg gtggtcttct ccg                                               23


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer

<400>  6
gaaagtgcag gagagccagg                                                   20


<210>  7
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer

<400>  7
cgattaatat ggctctggtt ctgg                                              24


<210>  8
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer

<400>  8
cacaaaggga gttttccaca cggac                                             25


<210>  9
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer

<400>  9
cacgacctgc tggttctcag tgg                                               23


<210>  10
<211>  100
<212>  RNA
<213>  Artificial sequence

<220>
<223>  sgRNA


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  methylated bases

<220>
<221>  misc_feature
<222>  (100)..(102)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (100)..(102)
<223>  methylated bases

<400>  10
agaggaacaa uacaaauucg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  11
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  11
ccgctgtttc aacagaaatg acc                                               23


<210>  12
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer

<400>  12
ccaactcagt gactgcggtc g                                                 21


<210>  13
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer ptwB_5996

<400>  13
gcggcgtgcg gtacccatgt tcttaattaa atttttc                                37


<210>  14
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer ptwB_6082

<400>  14
gcgctggcgg tacccctata gtgagtcgta ttatac                                 36


<210>  15
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer pBRL834_352-attP_F

<400>  15
gcgcgcggct tataaggttt gtctggtcaa cca                                    33


<210>  16
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer pBRL834_attP_R

<400>  16
gcgcgcggct tataaggttt gtaccgtaca ccac                                   34


<210>  17
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer pYUB328-cosBF

<400>  17
gcgcgttagt catgagctag ccgatccgcc ttgttacggg g                           41


<210>  18
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer pYUB328-cosBR

<400>  18
gcgcgcgttc gtcatgaatt taaatacgtg tctagatacg tctgc                       45


<210>  19
<211>  13892
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid insert in AASV1

<400>  19
gtacaggcat ccctgtgaaa gatgcctgag gcctgggcac cagggactcc gaagtccagg       60

cccagcccct ccccatttaa cccaggaggc caggcccatc ccctccccca ctcacccagg      120

aggccaggcc ccagcctttc catcctcaga tgcagaagcc caggccccca gcctctcccc      180

attcagaccc aggagtccag gcccccagcc cctcctccct gagacccagg agtccaggcc      240

cccagcccct cctccctgag acccaggagt ccaggccaca gcccctcctc cctcaaaccc      300

aggagcccag gaccccagct cctcctccct cagacccagg agtccaggct ccagcccctg      360

ctccctcaga tccaggagtc caggctccag ctcctgctcc ctcagaccca ggagtccagg      420

ttccagcccc ctcctccctc agacccagga gcccaggcca cagcccctcc tccctcagac      480

tcagaagccc aggcccccag gtcttctctg ttcagcccta agaatcctgg ctcctagccc      540

ctcctactct agtccccaat cccctagcca ctaaggcagt gggagggcag gaatgcggga      600

agtgtaccag cctcaccaag tggttgataa aaccacatgg ggtaccctaa gaactcggaa      660

actgccatag cagggggctg atgcttgggg acctgcctca agaaggatgc aggagaaaca      720

cagccccagg tggagaaact ggccgggact caagagtcac ccagagacag tgaccccgtc      780

cttgttttca taggacttag ggtttcagcg ctaaaaccag gctgttatgg gcaaacgtca      840

taagctggtc accccacacc cagacctgac ccaaacccag ctcccttgct tgctggccac      900

gtaacctgag aagggaatcc cctcttccct gaactccagc ccaccccaat actccaggcc      960

tcctgggata ccccgaggag tgagtttgcc aagcagtcac cccacagtgg gaggagaatc     1020

cacctgaaag ccagcctggt ggactgggct ggggcgacct ctcgccgggt ccaggccaac     1080

taggtggccc ggggcctctg ggggatgcag gggaaggggg ctcagtctga agagcagagc     1140

caggaacccc tgtagggaaa gtgcaggaga gccaggggca tgggatggtg acgaggaaga     1200

gggacaggga atctgagcgc ctctcctggg cttgccaggg actcaaaccc agaggcccag     1260

agcagggcct tagggaagag ggaccccggg accccgctcc gggcggagga gtatgtccca     1320

gacagcactg gggctcttta aggaaagaag gatggagaaa gagaaggggg tagaggcggc     1380

catgacctgg tgaacaccta ggacgaacca ttctcacaaa gggagttttc cacacggaca     1440

cgccctcctc gccacagccc tgccagggcg gggccggcta ctggccttat ctcacaggga     1500

aaactgatgc acagaggaac aatacaaatg gcttgtcgac gacggcggtc tcagtggtgt     1560

acggtacaaa ccttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa     1620

agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca     1680

tgtctgtacc gctgtttcaa cagaaatgac cgcccccggg gggcggtgct gtttgcgggt     1740

tggcacaaaa agaccccgac ccgcgtctgt ggtgtttttg gcatcatgtc gccgggcgcc     1800

atgcgtgccg ttgttcccat tatcccattc cttttggttc ttgtcggtgt atcgggggtt     1860

cccaccaacg tctcctccac cacccaaccc caactccaga ccaccggtcg tccctcgcat     1920

gaagccccca acatgaccca gaccggcacc accgactctc ccaccgccat cagccttacc     1980

acgcccgacc acacaccccc catgccaagt atcggactgg aggaggagga ggaagaggag     2040

gagggggccg gggatggcga acatcttaag gggggagatg ggacccgtga caccctaccc     2100

cagtccccgg gtccagccgt cccgttggcc ggggatgacg agaaggacaa acccaaccgt     2160

cccgtagtcc caccccccgg tcccaacaac tcccccgcgc gccccgagac cagtcgaccg     2220

aagacacccc ccaccagtat cgggccgctg gcaactcgac ccacgaccca actcccctca     2280

aaggggcgac ccttggttcc gacgcctcaa cataccccgc tgttctcgtt cctcactgcc     2340

tcccccgccc tggacaccct cttcgtcgtc agcaccgtca tccacacctt atcgtttgtg     2400

tgtattgttg ctatggcgac acacctgtgt ggtggttggt ccagacgcgg gcgacgcaca     2460

caccctagcg tgcgttacgt gtgcctgccg cccgaacgcg ggtagggtat ggggcgggga     2520

tggggagagc ccacacgcgg aaagcaagaa caataaaggc ggcgggatct agttgatatg     2580

cgtctctggg tgtttttggg gtgtggtggg cgcggggcgg tcattggacg ggggtgcagt     2640

taaatacatg cccgggaccc atgaagcatg cgcgacttcc gggcctcgga acccacccga     2700

aacggccaac ggacgtctga gccaggcctg gctatccgga gaaacagcac acgacttggc     2760

gttctgtgtg tcgcgatgtc tctgcgcgca gtctggcatc tggggctttt gggaagcctc     2820

gtgggggctg ttcttgccgc cacccatctg ggacctgcgg ccaacacaac ggacccctta     2880

acgcacgccc cagtgtcccc tcaccccagc cccctggggg gctttgccgt ccccctcgta     2940

gtcggtgggc tgtgtgccgt agtcctgggg gcggcgtgtc tgcttgagct cctgcgtcgt     3000

acgtgccgcg ggtgggggcg ttaccatccc tacatggacc cagttgtcgt ataatttttt     3060

cccccccccc cttctccgca tgggtgatgt cgggtccaaa ctcccgacac caccagctgg     3120

catggtataa atcaccggtg cgccccccaa accatgtccg gcagggggat ggggggcgaa     3180

tgcggagggc acccaacaac accgggctaa ccaggaaatc cgtggccccg gcccccaaca     3240

aagatcgcgg tagcccggcc gtgtgacatt atcgtccata ccgaccacac cgacgaatcc     3300

cctaaggggg aggggccatt ttacgaggag gaggggtata acaaagtctg tctttaaaaa     3360

gcaggggtta gggagttgtt cggtcataag cttcagtgcg aacgaccaac taccccgatc     3420

atcagttatc cttaaggtct cttttgtgtg gtgcgttccg gtatgggggg ggctgccgcc     3480

aggttggggg ccgtgatttt gtttgtcgtc atagtgggcc tccatggggt ccgcggcaaa     3540

tatgccttgg cggatgcctc tctcaagatg gccgacccca atcgctttcg cggcaaagac     3600

cttccggtcc tggaccagct gaccgaccct ccgggggtcc ggcgcgtgta ccacatccag     3660

gcgggcctac cggacccgtt ccagcccccc agcctcccga tcacggttta ctacgccgtg     3720

ttggagcgcg cctgccgcag cgtgctccta aacgcaccgt cggaggcccc ccagattgtc     3780

cgcggggcct ccgaagacgt ccggaaacaa ccctacaacc tgaccatcgc ttggtttcgg     3840

atgggaggca actgtgctat ccccatcacg gtcatggagt acaccgaatg ctcctacaac     3900

aagtctctgg gggcctgtcc catccgaacg cagccccgct ggaactacta tgacagcttc     3960

agcgccgtca gcgaggataa cctggggttc ctgatgcacg cccccgcgtt tgagaccgcc     4020

ggcacgtacc tgcggctcgt gaagataaac gactggacgg agattacaca gtttatcctg     4080

gagcaccgag ccaagggctc ctgtaagtac gccctcccgc tgcgcatccc cccgtcagcc     4140

tgcctctccc cccaggccta ccagcagggg gtgacggtgg acagcatcgg gatgctgccc     4200

cgcttcatcc ccgagaacca gcgcaccgtc gccgtataca gcttgaagat cgccgggtgg     4260

cacgggccca aggccccata cacgagcacc ctgctgcccc cggagctgtc cgagaccccc     4320

aacgccacgc agccagaact cgccccggaa gaccccgagg attcggccct cttggaggac     4380

cccgtgggga cggtggcgcc gcaaatccca ccaaactggc acatcccgtc gatccaggac     4440

gccgcgacgc cttaccatcc cccggccacc ccgaacaaca tgggcctgat cgccggcgcg     4500

gtgggcggca gtctcctggc agccctggtc atttgcggaa ttgtgtactg gatgcaccgc     4560

cgcactcgga aagccccaaa gcgcatacgc ctcccccaca tccgggaaga cgaccagccg     4620

tcctcgcacc agcccttgtt ttactagata ccccccctta atgggtgcgg gggggtcagg     4680

tctgcggggt tgggatggga ccttaactcc atataaagcg agtctggaag gggggaaagg     4740

cggacagtcg ataagtcggt agcgggggac gcgcacctgt tccgcctgtc gcacccacag     4800

ctttttcgcg aaccgtcccg tttcgggatg ccgtgccgcc cgttgcaggg cctggtgctc     4860

gtgggcctct gggtctgtgc caccagcctg gttgtccgtg gccccacggt cagtctggta     4920

tcaaactcat ttgtggacgc cggggccttg gggcccgacg gcgtagtgga ggaagacctg     4980

cttattctcg gggagcttcg ctttgtgggg gaccaggtcc cccacaccac ctactacgat     5040

ggggtcgtag agctgtggca ctaccccatg ggacacaaat gcccacgggt cgtgcatgtc     5100

gtcacggtga ccgcgtgccc acgtcgcccc gccgtggcat ttgccctgtg tcgcgcgacc     5160

gacagcactc acagccccgc atatcccacc ctggagctga atctggccca acagccgctt     5220

ttgcgggtcc ggagggcgac gcgtgactat gccggggtgt acgtgttacg cgtatgggtc     5280

ggggacgcac caaacgccag cctgtttgtc ctggggatgg ccatagccgc cgaaggtact     5340

ctggcgtaca acggctcggc ccatggctcc tgcgacccga aactgcttcc gtcttcggcc     5400

ccgcgtctgg ccccggcgag cgtataccaa cccgccccta acccggcctc caccccctcg     5460

accaccacct ccaccccctc gaccaccatc cccgctcccc aagcatcgac cacacccttc     5520

cccacgggag acccaaaacc ccaacctcac ggggtcaacc acgaaccccc atcgaatgcc     5580

acgcgagcga cccgcgactc gcgatatgcg ctaacggtga cccagataat ccagatagcc     5640

atccccgcgt ccattatagc cctggtgttt ctggggagct gtatttgctt tatacacaga     5700

tgtcaacgcc gctaccgacg ctcccgccgc ccgatttaca gcccccagat acccacgggc     5760

atctcatgcg cggtgaacga agcggccatg gcccgcctcg gagccgagct caaatcgcat     5820

ccgagcaccc cccccaaatc ccggcgccgg tcgtcacgca cgccaatgcc ctccctgacg     5880

gccatcgccg aagagtcgga gcccgcgggg gcggctgggc ttccgacgcc ccccgtggac     5940

cccacgacat ccaccccaac gcctcccctg ttggtatagg tccacggcca ctggccgggg     6000

gcaccacata accgaccgca gtcactgagt ttaccgtcga cctctagcta gagcttggcg     6060

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     6120

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     6180

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     6240

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     6300

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     6360

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     6420

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     6480

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     6540

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     6600

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     6660

tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     6720

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     6780

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     6840

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     6900

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     6960

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     7020

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     7080

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagctag     7140

ccgatccgcc ttgttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc     7200

cggtttaagg cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct     7260

ctgaaaagaa aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt     7320

tctctgtttt tgtccgtgga atgaacaatg gaagtcaaca aaaagcagac gtatctagac     7380

acgtatttaa attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     7440

gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc     7500

cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta     7560

tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac     7620

aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc     7680

gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctaggctttt     7740

gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg     7800

gaccttctag gtcttgaaag gagtgcctcg tgaggctccg gtgcccgtca gtgggcagag     7860

cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc     7920

tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt     7980

cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc     8040

aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc     8100

tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt     8160

cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga     8220

gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga     8280

atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat     8340

ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa     8400

gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc     8460

cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg     8520

tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg     8580

ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt     8640

cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt     8700

gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc     8760

acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg     8820

tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag     8880

actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag     8940

tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt     9000

caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag acccaagctg     9060

gctaggtaag cttggtaccc atgttcttaa ttaaattttt caaaagtagt tgacaattaa     9120

tcatcggcat agtatatcgg catagtataa tacgactcac tataggggta ccgagctcgg     9180

atccactagt ccagtgtggt ggaattgccc ttagggcaat tctgcagata tgagcttcaa     9240

taccctgatt gactggaaca gctgtagccc tgaacagcag cgtgcgctgc tgacgcgtcc     9300

ggcgatttcc gcctctgaca gtattacccg gacggtcagc gatattctgg ataatgtaaa     9360

aacgcgcggt gacgatgccc tgcgtgaata cagcgctaaa tttgataaaa cagaagtgac     9420

agcgctacgc attacccctg aagagatcgc cgccgccggc gcgcgtctga gcgacgaatt     9480

aaaacaggcg atggccgctg ccgtcaaaaa tattgaaacg ttccattccg cgcagacgct     9540

accgcctgta gatgtggaaa cccagccagg cgtgcgttgc cagcaggtta cgcgtcccgt     9600

cgcgtctgtc ggtctgtata ttcccggcgg ctcggctccg ctcttctcaa cggtgctgat     9660

gctggcgacg ccggcgcgta ttgcgggatg tcagaacgtg gttctgtgct cgccgccgcc     9720

catcgctgat gaaatcctct atgcggcgca actgtgtggc gtgcaggaaa tctttaacgt     9780

cggcggcgcg caggcgattg ccgctctggc cttcggcagc gagtccgtac cgaaagtgga     9840

taaaattttt ggccccggca acgcctttgt aaccgaagcc aagcgtcagg tcagccagcg     9900

cctcgacggc gcggctatcg atatgccagc cgggccgtct gaagtactgg tgatagccga     9960

cagcggcgca acaccggatt tcgtcgcgtc tgacctgctc tcccaggctg agcacggtcc    10020

ggattcccag gtgattctgc tgacgcctga tgctgacatt gcccgcaagg tggcggaggc    10080

ggtagaacgt caactggcgg agctgccgcg cgcggacacc gcccggcagg ccctgagcgc    10140

cagtcgtctg attgtgacca aagatttagc gcagtgcgtc gccatctcta atcagtatgg    10200

gccggaacac ttaatcatcc agacgcgcaa tgcgcgcgat ttggtggatg cgattaccag    10260

cgcaggctcg gtatttctcg gcgactggtc gccggaatcc gccggtgatt acgcttccgg    10320

aaccaaccat gttttaccga cctatggcta tactgctacc tgttccagcc ttgggttagc    10380

ggatttccag aaacggatga ccgttcagga actgtcgaaa gcgggctttt ccgctctggc    10440

atcaaccatt gaaacattgg cggcggcaga acgtctgacc gcccataaaa atgccgtgac    10500

cctgcgcgta aacgccctca aggagcaagc atgagctcga gtctagaggg cccgcggttc    10560

gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat    10620

caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag    10680

ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact    10740

gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt    10800

ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat    10860

gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg    10920

gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc    10980

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc    11040

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg    11100

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca    11160

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc    11220

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct    11280

tttgatttat aaggtttgtc tggtcaacca ccgcggtctc cgtcgtcagg atcattcggg    11340

gctagaaagg tgaagaccca aaattagaac tcaggaccaa cttattctga ttttgttttt    11400

ccaaactgct tctcctcttg ggaagtgtaa ggaagcagca gcacccggat cagtgaaatg    11460

caccagacgg ctgcgccaga gcagctcagg ctccgggaga gggcagcgca gggcggccac    11520

tgagaaccag caggtcgtgc atcccccttc cctcccaccc cccgccaagc tctccctccc    11580

aggaccctct ctggctccat cagtgagcaa accctagggg ttctggcggg gagagagagg    11640

tggctccggg aaatgggggt gtgtcaccag ataaggaatc tgcttaaaag gaggtggggg    11700

tcagacccaa tatctggaga ctaggaggga ggaggcctaa ggatggggct tttctgtcac    11760

cagtcctgtc cctagtggct ccactggggg ggggtggagg ggacagataa acgtacccag    11820

aaccagagcc atattaatcg gccctgggaa tagaaggcgg tcccggctct gggacacagg    11880

atcctgtctg gaagtggcca tgggggcccg gcttggagga agagggctag ccgagctctg    11940

ggacccctgc aagatgccgt gacaggggcc ggaagagcga gaggtaaagg gggtatggga    12000

gctgcccaaa ttaaagaagt gagaggtgac ccaaatccac aggaggaggg ggtgtccagg    12060

caaagaaagc aagaggatgg agaggtggct aaagccaggg agacagggac tttggggttg    12120

tccagaaaaa cggtgatgat gcaggcctac aagaagggga ggtggggatg caagggacag    12180

acatcccacg gagaagacca ccctaagaaa cgagagatgg cacaggcccc agaaggagaa    12240

ggaaaaggga acccagcgag tgaagatggc atgggggttg gggtagggag cagagatgcc    12300

cggagaggac ccagacaagg gcaggatccg ctccgaggac accacgtggt gcagcgccga    12360

gaaggaagtg ctctggaaag agcatccttg ggcagcaacg cacagcacag agcaagggga    12420

agagggattg gaggaagctg gaacctgaag gaggtggtgg ggaaggatct gggccgagcc    12480

gtggaggtgg cccaggccac aatctgcagg cagaaagcag cacaggttca ggggagagaa    12540

tgcaggtcag tgaaagcagg acctgcctgg aaaggggaaa cagcgggcca gaggcggcgc    12600

agaagccagt agagctcaaa gtggtccgga ctccggagag agacggccgc gttagagggc    12660

agagtagagg cggcacagca agggcactcg gcggcgagag gggggcagcg caaagtgaca    12720

atggccaggg ccagggagat agaccagagt gagctatggg agctgcctca ggttcaggag    12780

agggcggggc agggagggag acaaagtcca gggccggcta gagaggctca acatcggaag    12840

aggggaagtc gagggaggga tggtaagggg gaccgcaggg gtcagcacag gctggcaaag    12900

ccagggccag ttaaagcgac tccaatgcgg aagacggtag gtcgaagggg actggtaagg    12960

aggcctgggg cagggtggta agcacagagt ggcaaagccc ggggccactt gaagcagccc    13020

caattcggaa gcggggtggt cgaagagggc tggtaagggg gcgctgggac ggggtgtcag    13080

catagggtgg caaagcccag ggctaggaac gacgggcgga tcgagactgg caacggcggc    13140

tcggaaccgg ggaaggagga tgccccaggc ggcgctgcgg agggtgggcc tggccccggg    13200

agacgctgga gggggcgctg acctggtgca gggcgctgat gccgtcggcg ttggtggagt    13260

ccagcacggc gcgggcgggc ggcggcgcgg cggggtcgag ctcggcgccg gggccagggt    13320

cggcggcgca cagcatcaga cgcgcttcgt ccaggtcgcc gcccgcacag gccgccagga    13380

actcggcggc gcgctcgaag cggacggtgc gggcgcggcg ctctccgggg ccaggctggg    13440

ggatgtcaca gaggctcctc ggagccagca gccgccgccg ccgcccccgg gccagccgcc    13500

gggccatcct ctccggacat cgcaccgccc gcccgcccag cgagcgagcg agcgagcgcc    13560

gagccccaac tgccgccgcc gccgcccgcc cgccccgggg gccgccggga actgccgctg    13620

gccccccacc gccccaggga tctcccggcc cccgcccggc gcgccgacgt cacggcgctg    13680

ccccagggtg tgctgggcag gtcgcgggga gcgccgggaa atggagtcca taagcggaag    13740

ttgcccctgg ccactttcag gagtcgctgt gccccgatgc acactgggaa gtccgcagct    13800

ccgaggcgcc cagtggaaat caccgaatga gggcctcctc cggggaatgc tgggaaatgg    13860

agtctacaga ccggaggggt gccccacggc at                                  13892


<210>  20
<211>  100
<212>  RNA
<213>  Artificial sequence

<220>
<223>  sgRNA


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Methylated bases

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  Methylated bases

<400>  20
ccugcaagau gccgugacag guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  21
<211>  100
<212>  RNA
<213>  Artificial sequence

<220>
<223>  sgRNA


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  Methylated bases

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  Phosphorothiolate bonds

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  Methylated bases

<400>  21
ccacccuaag aaacgagaga guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  22
<211>  4086
<212>  DNA
<213>  Vero AAVS1 sequence

<400>  22
gtacaggcat ccctgtgaaa gatgcctgag gcctgggcac cagggactcc gaagtccagg       60

cccagcccct ccccatttaa cccaggaggc caggcccatc ccctccccca ctcacccagg      120

aggccaggcc ccagcctttc catcctcaga tgcagaagcc caggccccca gcctctcccc      180

attcagaccc aggagtccag gcccccagcc cctcctccct gagacccagg agtccaggcc      240

cccagcccct cctccctgag acccaggagt ccaggccaca gcccctcctc cctcaaaccc      300

aggagcccag gaccccagct cctcctccct cagacccagg agtccaggct ccagcccctg      360

ctccctcaga tccaggagtc caggctccag ctcctgctcc ctcagaccca ggagtccagg      420

ttccagcccc ctcctccctc agacccagga gcccaggcca cagcccctcc tccctcagac      480

tcagaagccc aggcccccag gtcttctctg ttcagcccta agaatcctgg ctcctagccc      540

ctcctactct agtccccaat cccctagcca ctaaggcagt gggagggcag gaatgcggga      600

agtgtaccag cctcaccaag tggttgataa aaccacatgg ggtaccctaa gaactcggaa      660

actgccatag cagggggctg atgcttgggg acctgcctca agaaggatgc aggagaaaca      720

cagccccagg tggagaaact ggccgggact caagagtcac ccagagacag tgaccccgtc      780

cttgttttca taggacttag ggtttcagcg ctaaaaccag gctgttatgg gcaaacgtca      840

taagctggtc accccacacc cagacctgac ccaaacccag ctcccttgct tgctggccac      900

gtaacctgag aagggaatcc cctcttccct gaactccagc ccaccccaat actccaggcc      960

tcctgggata ccccgaggag tgagtttgcc aagcagtcac cccacagtgg gaggagaatc     1020

cacctgaaag ccagcctggt ggactgggct ggggcgacct ctcgccgggt ccaggccaac     1080

taggtggccc ggggcctctg ggggatgcag gggaaggggg ctcagtctga agagcagagc     1140

caggaacccc tgtagggaaa gtgcaggaga gccaggggca tgggatggtg acgaggaaga     1200

gggacaggga atctgagcgc ctctcctggg cttgccaggg actcaaaccc agaggcccag     1260

agcagggcct tagggaagag ggaccccggg accccgctcc gggcggagga gtatgtccca     1320

gacagcactg gggctcttta aggaaagaag gatggagaaa gagaaggggg tagaggcggc     1380

catgacctgg tgaacaccta ggacgaacca ttctcacaaa gggagttttc cacacggaca     1440

cgccctcctc gccacagccc tgccagggcg gggccggcta ctggccttat ctcacaggga     1500

aaactgatgc acagaggaac aatacaaatt cggggctaga aaggtgaaga cccaaaatta     1560

gaactcagga ccaacttatt ctgattttgt ttttccaaac tgcttctcct cttgggaagt     1620

gtaaggaagc agcagcaccc ggatcagtga aatgcaccag acggctgcgc cagagcagct     1680

caggctccgg gagagggcag cgcagggcgg ccactgagaa ccagcaggtc gtgcatcccc     1740

cttccctccc accccccgcc aagctctccc tcccaggacc ctctctggct ccatcagtga     1800

gcaaacccta ggggttctgg cggggagaga gaggtggctc cgggaaatgg gggtgtgtca     1860

ccagataagg aatctgctta aaaggaggtg ggggtcagac ccaatatctg gagactagga     1920

gggaggaggc ctaaggatgg ggcttttctg tcaccagtcc tgtccctagt ggctccactg     1980

ggggggggtg gaggggacag ataaacgtac ccagaaccag agccatatta atcggccctg     2040

ggaatagaag gcggtcccgg ctctgggaca caggatcctg tctggaagtg gccatggggg     2100

cccggcttgg aggaagaggg ctagccgagc tctgggaccc ctgcaagatg ccgtgacagg     2160

ggccggaaga gcgagaggta aagggggtat gggagctgcc caaattaaag aagtgagagg     2220

tgacccaaat ccacaggagg agggggtgtc caggcaaaga aagcaagagg atggagaggt     2280

ggctaaagcc agggagacag ggactttggg gttgtccaga aaaacggtga tgatgcaggc     2340

ctacaagaag gggaggtggg gatgcaaggg acagacatcc cacggagaag accaccctaa     2400

gaaacgagag atggcacagg ccccagaagg agaaggaaaa gggaacccag cgagtgaaga     2460

tggcatgggg gttggggtag ggagcagaga tgcccggaga ggacccagac aagggcagga     2520

tccgctccga ggacaccacg tggtgcagcg ccgagaagga agtgctctgg aaagagcatc     2580

cttgggcagc aacgcacagc acagagcaag gggaagaggg attggaggaa gctggaacct     2640

gaaggaggtg gtggggaagg atctgggccg agccgtggag gtggcccagg ccacaatctg     2700

caggcagaaa gcagcacagg ttcaggggag agaatgcagg tcagtgaaag caggacctgc     2760

ctggaaaggg gaaacagcgg gccagaggcg gcgcagaagc cagtagagct caaagtggtc     2820

cggactccgg agagagacgg ccgcgttaga gggcagagta gaggcggcac agcaagggca     2880

ctcggcggcg agaggggggc agcgcaaagt gacaatggcc agggccaggg agatagacca     2940

gagtgagcta tgggagctgc ctcaggttca ggagagggcg gggcagggag ggagacaaag     3000

tccagggccg gctagagagg ctcaacatcg gaagagggga agtcgaggga gggatggtaa     3060

gggggaccgc aggggtcagc acaggctggc aaagccaggg ccagttaaag cgactccaat     3120

gcggaagacg gtaggtcgaa ggggactggt aaggaggcct ggggcagggt ggtaagcaca     3180

gagtggcaaa gcccggggcc acttgaagca gccccaattc ggaagcgggg tggtcgaaga     3240

gggctggtaa gggggcgctg ggacggggtg tcagcatagg gtggcaaagc ccagggctag     3300

gaacgacggg cggatcgaga ctggcaacgg cggctcggaa ccggggaagg aggatgcccc     3360

aggcggcgct gcggagggtg ggcctggccc cgggagacgc tggagggggc gctgacctgg     3420

tgcagggcgc tgatgccgtc ggcgttggtg gagtccagca cggcgcgggc gggcggcggc     3480

gcggcggggt cgagctcggc gccggggcca gggtcggcgg cgcacagcat cagacgcgct     3540

tcgtccaggt cgccgcccgc acaggccgcc aggaactcgg cggcgcgctc gaagcggacg     3600

gtgcgggcgc ggcgctctcc ggggccaggc tgggggatgt cacagaggct cctcggagcc     3660

agcagccgcc gccgccgccc ccgggccagc cgccgggcca tcctctccgg acatcgcacc     3720

gcccgcccgc ccagcgagcg agcgagcgag cgccgagccc caactgccgc cgccgccgcc     3780

cgcccgcccc gggggccgcc gggaactgcc gctggccccc caccgcccca gggatctccc     3840

ggcccccgcc cggcgcgccg acgtcacggc gctgccccag ggtgtgctgg gcaggtcgcg     3900

gggagcgccg ggaaatggag tccataagcg gaagttgccc ctggccactt tcaggagtcg     3960

ctgtgccccg atgcacactg ggaagtccgc agctccgagg cgcccagtgg aaatcaccga     4020

atgagggcct cctccgggga atgctgggaa atggagtcta cagaccggag gggtgcccca     4080

cggcat                                                                4086


<210>  23
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 1231F

<400>  23
cttgccaggg actcaaaccc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer attR

<400>  24
atgatcctga cgacggagac                                                   20


<210>  25
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 1529attB_F

<400>  25
gtcgacgacg gcggtctc                                                     18


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 1529attB_R

<400>  26
gaccgccttc tattcccagg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 1201F

<400>  27
aatctgagcg cctctcctgg                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  primer 2471R

<400>  28
cccccatgcc atcttcactc                                                   20


<210>  29
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  attL

<400>  29
ttgtcgacga cggcggtctc agtggtgtac ggtacaaacc                             40


<210>  30
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  attR

<400>  30
ggtttgtctg gtcaaccacc gcggtctccg tcgtcaggat cat                         43


