                         SEQUENCE LISTING

<110>  Anna, Huttenlocher
       Mosher, Deane
       Bortnov, Valeriu
       Bennin, David
 
<120>  Method to Inhibit Neutrophil Recruitment to Damaged Tissue Using 
       Myeloid-Derived Growth Factor

<130>  09824.315 / P2100666US02

<140>  xx/yyy,zzz
<141>  2021-09-22

<150>  63/081,664
<151>  2020-09-22

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  173
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  MYDGF Amino Acid Sequence, Human (UniProtKB - Q969H8):

<220>
<221>  SIGNAL
<222>  (1)..(31)

<220>
<221>  mat_peptide
<222>  (32)..(173)

<400>  1

Met Ala Ala Pro Ser Gly Gly Trp Asn Gly Val Gly Ala Ser Leu Trp 
    -30                 -25                 -20                 


Ala Ala Leu Leu Leu Gly Ala Val Ala Leu Arg Pro Ala Glu Ala Val 
-15                 -10                 -5              -1  1   


Ser Glu Pro Thr Thr Val Ala Phe Asp Val Arg Pro Gly Gly Val Val 
            5                   10                  15          


His Ser Phe Ser His Asn Val Gly Pro Gly Asp Lys Tyr Thr Cys Met 
        20                  25                  30              


Phe Thr Tyr Ala Ser Gln Gly Gly Thr Asn Glu Gln Trp Gln Met Ser 
    35                  40                  45                  


Leu Gly Thr Ser Glu Asp His Gln His Phe Thr Cys Thr Ile Trp Arg 
50                  55                  60                  65  


Pro Gln Gly Lys Ser Tyr Leu Tyr Phe Thr Gln Phe Lys Ala Glu Val 
                70                  75                  80      


Arg Gly Ala Glu Ile Glu Tyr Ala Met Ala Tyr Ser Lys Ala Ala Phe 
            85                  90                  95          


Glu Arg Glu Ser Asp Val Pro Leu Lys Thr Glu Glu Phe Glu Val Thr 
        100                 105                 110             


Lys Thr Ala Val Ala His Arg Pro Gly Ala Phe Lys Ala Glu Leu Ser 
    115                 120                 125                 


Lys Leu Val Ile Val Ala Lys Ala Ser Arg Thr Glu Leu 
130                 135                 140         


<210>  2
<211>  990
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  NCBI Reference Sequence: NM_019107.4

<400>  2
agtccaacat ggcggcgccc agcggagggt ggaacggcgt cggcgcgagc ttgtgggccg       60

cgctgctcct aggggccgtg gcgctgaggc cggcggaggc ggtgtccgag cccacgacgg      120

tggcgtttga cgtgcggccc ggcggcgtcg tgcattcctt ctcccataac gtgggcccgg      180

gggacaaata tacgtgtatg ttcacttacg cctctcaagg agggaccaat gagcaatggc      240

agatgagtct ggggaccagc gaagaccacc agcacttcac ctgcaccatc tggaggcccc      300

aggggaagtc ctatctgtac ttcacacagt tcaaggcaga ggtgcggggc gctgagattg      360

agtacgccat ggcctactct aaagccgcat ttgaaaggga aagtgatgtc cctctgaaaa      420

ctgaggaatt tgaagtgacc aaaacagcag tggctcacag gcccggggca ttcaaagctg      480

agctgtccaa gctggtgatt gtggccaagg catcgcgcac tgagctgtga ccagcagccc      540

tgttgcgggt ggcaccttct catctccggt gaagctgaag gggcctgtgt ccctgaaagg      600

gccagcacat cactggtttt ctaggaggga ctcttaagtt ttctacctgg gctgacgttg      660

ccttgtccgg aggggcttgc agggtggctg aagccctggg gcagagaaca gagggtccag      720

ggccctcctg gctcccaaca gcttctcagt tcccacttcc tgctgagctc ttctggactc      780

aggatcgcag atccggggca caaagagggt ggggaacatg ggggctatgc tggggaaagc      840

agccatgctc cccccgacct ccagccgagc atccttcatg agcctgcaga actgctttcc      900

tatgtttacc caggggacct cctttcagat gaactgggaa gagatgaaat gttttttcat      960

atttaaataa ataagaacat taaaaagcaa                                       990


<210>  3
<211>  166
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  MYDGF Amino Acid Sequence, Mouse (UniProtKB - Q9CPT4)

<400>  3

Met Ala Ala Pro Ser Gly Gly Phe Trp Thr Ala Val Val Leu Ala Ala 
1               5                   10                  15      


Ala Ala Leu Lys Leu Ala Ala Ala Val Ser Glu Pro Thr Thr Val Pro 
            20                  25                  30          


Phe Asp Val Arg Pro Gly Gly Val Val His Ser Phe Ser Gln Asp Val 
        35                  40                  45              


Gly Pro Gly Asn Lys Phe Thr Cys Thr Phe Thr Tyr Ala Ser Gln Gly 
    50                  55                  60                  


Gly Thr Asn Glu Gln Trp Gln Met Ser Leu Gly Thr Ser Glu Asp Ser 
65                  70                  75                  80  


Gln His Phe Thr Cys Thr Ile Trp Arg Pro Gln Gly Lys Ser Tyr Leu 
                85                  90                  95      


Tyr Phe Thr Gln Phe Lys Ala Glu Leu Arg Gly Ala Glu Ile Glu Tyr 
            100                 105                 110         


Ala Met Ala Tyr Ser Lys Ala Ala Phe Glu Arg Glu Ser Asp Val Pro 
        115                 120                 125             


Leu Lys Ser Glu Glu Phe Glu Val Thr Lys Thr Ala Val Ser His Arg 
    130                 135                 140                 


Pro Gly Ala Phe Lys Ala Glu Leu Ser Lys Leu Val Ile Val Ala Lys 
145                 150                 155                 160 


Ala Ala Arg Ser Glu Leu 
                165     


<210>  4
<211>  160
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  NCBI Reference Sequence: NM_080837.2 (Mouse MYDGF)

<400>  4

Met Ala Ala Pro Ser Gly Gly Phe Trp Thr Ala Val Val Leu Ala Ala 
1               5                   10                  15      


Ala Ala Leu Lys Leu Ala Ala Ala Val Ser Glu Pro Thr Thr Val Pro 
            20                  25                  30          


Phe Asp Val Arg Pro Gly Gly Val Val His Ser Phe Ser Gln Asp Val 
        35                  40                  45              


Gly Pro Gly Asn Lys Phe Thr Cys Thr Phe Thr Tyr Ala Ser Gln Gly 
    50                  55                  60                  


Gly Thr Asn Glu Gln Trp Gln Met Ser Leu Gly Thr Ser Glu Asp Ser 
65                  70                  75                  80  


Gln His Phe Thr Cys Thr Ile Trp Arg Pro Gln Gly Lys Ser Tyr Leu 
                85                  90                  95      


Tyr Phe Thr Gln Phe Lys Ala Glu Leu Arg Gly Ala Glu Ile Glu Tyr 
            100                 105                 110         


Ala Met Ala Tyr Ser Lys Ala Ala Phe Glu Arg Glu Ser Asp Val Pro 
        115                 120                 125             


Leu Lys Ser Glu Glu Phe Glu Val Thr Lys Thr Ala Val Ser His Arg 
    130                 135                 140                 


Pro Gly Ala Phe Lys Ala Glu Leu Ser Lys Leu Val Ile Val Ala Lys 
145                 150                 155                 160 


<210>  5
<211>  6
<212>  PRT
<213>  Mus musculus


<220>
<221>  misc_feature
<223>  Signal peptide for mouse MYDGF gene

<400>  5

Ala Ala Arg Ser Glu Leu 
1               5       


<210>  6
<211>  913
<212>  DNA
<213>  Mus musculus

<400>  6
gagcgcctgc gcattgcccc ggaagcaaga tggcagcccc cagcggaggc ttctggactg       60

cggtggtcct ggcggccgca gcgctgaaat tggccgccgc tgtgtccgag cccaccaccg      120

tgccatttga cgtgaggccc ggaggggtcg tgcattcgtt ctcccaggac gtaggacccg      180

ggaacaagtt tacatgtaca ttcacctacg cttcccaagg agggaccaac gagcaatggc      240

agatgagcct ggggacaagt gaagacagcc agcactttac ctgtaccatc tggaggcccc      300

aggggaaatc ctacctctac ttcacacagt tcaaggctga gttgcgaggt gctgagatcg      360

agtatgccat ggcctactcc aaagccgcat ttgagagaga gagtgatgtc cccctgaaaa      420

gtgaggagtt tgaagtgacc aagacagcag tgtctcacag gcctggggcc ttcaaagctg      480

agctctccaa gctggtgatc gtagccaagg cggcacgctc ggagctgtga ccctcgcctg      540

tcaagggcct tcatgtccac gttcctcagg cacactgacc gggactactt gtctagggca      600

ctggttccca taggagctgc cctgccctgc acaggtcaca ctgtgtcact ccgcagaact      660

ctctgagccc ggtcacctgt tttgccaggg aagatgcagg gcatgtgcgg gggtgggatg      720

gaaggacttc ctggctttcc tgaagtcaag atgtggtgtg gtttcccctc tgagccacag      780

atgagtgtcc ccatcccagg accactttct aaccccatcc agggcagctc cactcagaag      840

gatgggaaag gatagaaaaa ataaataaat aagtagccac cttagtggtg gctctgtggg      900

gtcaggactc aga                                                         913


<210>  7
<211>  164
<212>  PRT
<213>  Danio rerio


<220>
<221>  misc_feature
<223>  MYDGF Amino Acid Sequence, Zebrafish (UniProtKB - A7MCH1)

<400>  7

Met Ala Phe Ile Val His Met Lys Trp Phe Val Asn Leu Leu Leu Leu 
1               5                   10                  15      


Phe Val Val Leu Cys Glu Leu Cys Ser Ala Glu Arg Thr Lys Thr Leu 
            20                  25                  30          


Asp Phe Asp Val Lys Pro Gly Gly Val Val Gln Thr Phe Ser Ala Lys 
        35                  40                  45              


Leu Lys Lys Tyr Lys Cys Thr Phe Thr Tyr Ala Cys Gln Gly Gly Thr 
    50                  55                  60                  


Asn Glu Gln Trp Gln Met Ser Val Gly Leu Ser Asp Asp Glu Gln Met 
65                  70                  75                  80  


Phe Ser Cys Ser Val Trp Arg Pro Gln Gly Lys Ser Tyr Leu Phe Phe 
                85                  90                  95      


Thr Gln Phe Lys Ala Glu Ile Lys Gly Ala Lys Ile Glu Tyr Ala Thr 
            100                 105                 110         


Ala Tyr Ser Gln Thr Ala Val Gly Gly Gln Arg Asp Val Ala Leu Lys 
        115                 120                 125             


Glu Glu Glu Tyr Ile Val Ser Glu Ser Ala Val Thr Gln Arg Asp Gly 
    130                 135                 140                 


Lys Phe His Ser Glu Leu Ser Lys Leu Thr Val Ile Gly Arg Ile Arg 
145                 150                 155                 160 


His Asp Glu Leu 
                


<210>  8
<211>  1473
<212>  DNA
<213>  Danio rerio

<400>  8
atcctatgaa gaacatacag gaacagtaca ggcacctttt tcattcacaa cataaacgca       60

gatctcctgt ctgtaaagca tcatggcatt tattgtgcac atgaaatggt ttgtgaatct      120

tctgctgctg tttgttgtgc tttgtgaact gtgttctgct gaaaggacca aaacactgga      180

cttcgatgtc aaacctggag gagttgtgca gactttctct gcaaaactta agaagtataa      240

atgcaccttc acatatgcat gccaaggagg aaccaatgag caatggcaaa tgagtgtcgg      300

actaagtgac gatgagcaaa tgttttcctg ttcagtatgg aggccccaag ggaagtccta      360

cttgtttttt acgcagttca aagccgagat aaaaggagcc aagatcgagt acgccaccgc      420

atattcccag acggccgtgg gtggacagag ggatgttgct ttgaaagaag aagagtacat      480

agtgtcagag tctgcagtga cacaaagaga tggaaaattc cattcggagc tttctaagct      540

cactgtcatt ggtcgaatac ggcatgatga actctgattg gccgattcga aggacgttgg      600

ttatttaaca gctttggaca caattttctg ctcacggtca taagtcaagc gaaggaaatc      660

acagcactgg acattctgaa aaatgaattt accgtgatag aaaagatttc ctatagatgc      720

cagtggccaa aggttggagt gatcatcaga ggtagacgaa aagtctatta ttggatatta      780

taaggggtaa aacaaattgt acatgcgcat ttttttcata tttgtaaggt gttataaagt      840

cttctttata ttcagtgcag aacagcagat tgatttctgt tcttgtcaga atttatgggt      900

tttctagggt tacagtctga ctttgattca tacctgcaaa gctgattatc aagagtgctc      960

atacttactt tcctaggctg gtgtaaaggc acgcttgtag acaattccca ctaaatcttt     1020

ggggtgtgtt agaatccttt gagtatttct gtggtggagg aaaaaacatc aagttggtta     1080

ctacagttcc ttttgtctcc cagtcatgct ttattctgat aaccgatgta tcggaatttg     1140

aataaatctg ttatcagaac aatttgactt ctggccttac tatttgctga ctttgtatac     1200

ctaatatttt tgcgtaaagt aagtgaattg tgtctgttta aaagcaacaa cctctgaggg     1260

ctgttttttt tgcactctta agggtgaaaa atgtgtgtaa ttctgctgca taaaattaaa     1320

ataagctaca aatttgctga ttctgttcaa gatttgtcca gtcagctctt taaaaggctt     1380

gtcggtaccc caactgtgaa taaaagagcc tgcaatgcaa ttcacaagct attaaagaaa     1440

ttaatgtctt gagaaccaaa aaaaaaaaaa aaa                                  1473


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  9
cccaagggaa gtcctacttg                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  10
agcaacatcc ctctgtccac                                                   20


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  11
cgctgcgtca cctgtatt                                                     18


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  12
tagcatacga cggctgaact                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  13
tgccttcgtc ccaatttcag                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  14
taccctcctt gcgctcaatc                                                   20


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  15
gagtgtataa gttacctcca tactg                                             25


<210>  16
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  16
ggaatagcat atacgcatgc tcacc                                             25


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  17
cctcttacct cagttacaat ttata                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  18
ggattagctg atgtcatgtc cgaca                                             25


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  19
ggccgtgggt ggacagg                                                      17


<210>  20
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  20
gtaaaacgac ggccagtgtg acattgacat ttgccgca                               38


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  21
agactctgac actatgtact ct                                                22


<210>  22
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  22
ctgtgtggta ccgaaaggac caaaacactg gacttcg                                37


<210>  23
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Primer

<400>  23
tagagggcta gctcagagtt catcatgccg tattcgac                               38


<210>  24
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal polyhistidine tag and thrombin cleavage site

<400>  24

Met Gly Gly Ser His His His His His His Gly Ser Leu Val Pro Arg 
1               5                   10                  15      


Gly Ser Lys Gly Thr 
            20      


