			SEQUENCE LISTING


<110> KALAMAZOO HOLDINGS, INC.
 
<120> ENZYMATIC PROCESS FOR PRODUCTION OF MODIFIED HOP PRODUCTS
 
<130> KALSEC 75 PCT 2
 
<141> 2020-09-24

<150> 16/583,762
<151> 2019-09-26

<150> PCT/US2019/053117
<151> 2019-09-26


<160> 412
 
<210> 1
<211> 759
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild-Type Kred from Lactobacillus kefir
 
<400> 1
atgaccgatc gtctgaaggg caaagtagcc atcgtaaccg gcgggactct gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg catccgatga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg cagtttccaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttgaggggtt cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggctat atcaagaccc cgctggtcga tgatctggaa 600
ggtgctgagg aaatgatgtc acagcgtacg aaaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtatacc gcacagtga                        759

 
<210> 2
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Kred from Lactobacillus kefir
 

<400> 2
Met Thr Asp Arg Leu Lys Gly Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Asp Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Ala Val Ser 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Glu Gly Phe Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Tyr Ile Lys 
            180                 185                 190         
Thr Pro Leu Val Asp Asp Leu Glu Gly Ala Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Lys Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250                         

 
<210> 3
<211> 759
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 

<400> 3
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacagtga                        759

 
<210> 4
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 4
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         


<210> 5
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 5
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 6
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 6
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 7
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 7
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaggga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 8
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 8
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Arg Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 9
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 9
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgaggg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 10
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 10
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Arg Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 11
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 11
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta tttctgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 12
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 12
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ser Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 13
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 13
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgggtctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 14
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 14
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 15
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 15
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggcatga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 16
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 16
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg His Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 17
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 17
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcaggggttg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 18
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 18
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Val Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 19
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 19
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 20
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 20
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 21
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 21
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttggtgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 22
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 22
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Gly Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 23
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 23
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta tttgtgggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 24
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 24
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Cys Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 25
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 25
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacggttg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 26
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 26
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Val Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 27
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 27
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcag ggatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 28
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 28
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Arg Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 29
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 29
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
agggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 30
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 30
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Arg Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 31
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 31
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcaggggatg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 32
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 32
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Asp Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 33
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 33
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cattggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 34
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 34
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Ile Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 35
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 35
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgtgtg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 36
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 36
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Cys Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 37
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 37
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgcag 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 38
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 38
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Gln Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 39
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 39
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagacca ggcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 40
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 40
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Arg Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 41
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 41
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgcgg 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 42
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 42
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Arg Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 43
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 43
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatggtgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 44
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 44
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Val Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 45
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 45
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggatgg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 46
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 46
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Met Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 47
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 47
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcgg tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 48
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 48
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 49
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 49
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagacca atcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 50
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 50
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Asn Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 51
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 51
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctgttg 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 52
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 52
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Leu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 53
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 53
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 54
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 54
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 55
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 55
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggctgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 56
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 56
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Leu Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 57
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 57
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggacgg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 58
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 58
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Thr Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 59
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 59
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaagtccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 60
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 60
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Ser Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 61
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 61
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 62
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 62
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr His Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 63
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 63
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgtctctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 64
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 64
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 65
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 65
atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 66
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 66
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 67
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 67
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattgg gagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 68
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 68
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Gly Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 69
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 69
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgacctt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggctcga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 70
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 70
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Leu Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 71
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 71
atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 72
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 72
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 73
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 73
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 74
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 74
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 75
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 75
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 76
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 76
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 77
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 77
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 78
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 78
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 79
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 79
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 80
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 80
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 81
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 81
atgaccgatc gtctgaagca taaagtagcc atcattaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 82
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 82
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 83
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 83
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 84
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 84
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 85
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 85
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcataca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgggcctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaagg tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 86
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 86
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Gly Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 87
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 87
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagacca accggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 88
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 88
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Asn Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 89
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 89
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaacgccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 90
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 90
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 91
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 91
atgaccgatc gtctgaagca taaagtagcc atcgtaaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt cgtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgagcctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 92
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 92
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Ser Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 93
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 93
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaagg tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggctgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 94
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 94
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Gly Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Leu Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 95
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 95
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaactg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc atcggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 96
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 96
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Leu Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr His Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 97
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 97
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccgt ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttctggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc ctcggaaaga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 98
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 98
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Val Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Leu Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 99
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 99
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg cgtaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 100
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 100
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Arg Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 101
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 101
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaag tggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 102
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 102
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Ser Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 103
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 103
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 104
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 104
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 105
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 105
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgctctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 106
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 106
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 107
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 107
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgaagctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 108
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 108
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Lys Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 109
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 109
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
acgactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 110
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 110
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Thr Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 111
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 111
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaagtg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 112
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 112
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 113
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 113
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
tgtactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 114
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 114
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Cys Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 115
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 115
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 116
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 116
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 117
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 117
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgattgtat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 118
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 118
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Cys Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 119
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 119
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
gggggtgtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 120
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 120
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Gly Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 121
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 121
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg ggattattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 122
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 122
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ile Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 123
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 123
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgttgg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 124
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 124
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Trp Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 125
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 125
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtttt ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 126
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 126
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Phe Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 127
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 127
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
gctgcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 128
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 128
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Ala Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 129
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 129
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatcgt tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 130
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 130
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Val Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 131
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 131
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaagtggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 132
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 132
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 133
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 133
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
ctgactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 134
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 134
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Leu Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 135
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 135
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 136
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 136
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 137
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 137
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgttgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 138
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 138
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Leu Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 139
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 139
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgagttggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 140
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 140
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Leu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 141
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 141
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acggcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 142
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 142
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ala Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 143
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 143
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgct atcaagaccg agcgcaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 144
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 144
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 145
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 145
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggccct atcaagaccg agcgcaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 146
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 146
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 147
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 147
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatactg cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 148
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 148
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Cys Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 149
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 149
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 150
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 150
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 151
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 151
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaag tggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 152
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 152
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Ser Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 153
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 153
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggccct accaagaccg agcgcaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 154
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 154
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Thr Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 155
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 155
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgct accaagaccg agcgcaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 156
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 156
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Thr Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 157
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 157
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgctactaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 158
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 158
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Thr Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 159
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 159
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 160
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 160
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         

 
<210> 161
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 161
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacact gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgcgctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 162
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 162
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Leu Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250                         


<210> 163
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 163
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tgctctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 164
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 164
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 165
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 165
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccc atcaagaccc cgcggaagga tgctctggaa 600
ggttgggagg aaatgatgtc acagcgtacg cgtaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 166
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 166
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Ala Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Arg Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 167
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 167
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 168
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 168
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 169
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 169
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tgatctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 170
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 170
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Asp Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 171
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 171
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 172
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 172
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 173
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 173
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaca gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaagtagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 174
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 174
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Gln Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Val Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 175
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 175
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttgttaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcccg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ttaaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 176
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 176
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Val 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Pro Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 177
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 177
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagc agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 178
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 178
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 179
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 179
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catgggtgtg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 180
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 180
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Val Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 181
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 181
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
tatactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 182
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 182
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Tyr Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 183
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 183
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 184
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 184
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 185
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 185
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 186
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 186
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 187
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 187
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gggtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 188
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 188
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Gly Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 189
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 189
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 190
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 190
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 191
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 191
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtttgaaggc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
gacactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 192
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 192
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Asp Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 193
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 193
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 194
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 194
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 195
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 195
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 196
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 196
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 197
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 197
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 198
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 198
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 199
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 199
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 200
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 200
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 201
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 201
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 202
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 202
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 203
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 203
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcgcggggct cgtaggcgat ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 204
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 204
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Asp Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 205
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 205
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtagccaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 206
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 206
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ala 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 207
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 207
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 208
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 208
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 209
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 209
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcagcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 210
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 210
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Ala Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 211
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 211
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 212
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 212
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 213
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 213
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 214
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 214
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 215
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 215
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 216
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 216
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 217
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 217
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcagccgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 218
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 218
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ala 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 219
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 219
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcgcga tcgcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggtgctgagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 220
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 220
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ala Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Ala Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 221
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 221
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 222
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 222
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 223
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 223
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggtgctgagg aagcagcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 224
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 224
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Ala Glu Glu Ala Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 225
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 225
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcgcgg cggcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggtgctgagg aagcaatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 226
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 226
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ala Ala 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Ala Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 227
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 227
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcgcga tcgcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 228
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 228
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ala Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 229
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 229
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattgc gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcgcga tcgcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 230
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 230
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Ala Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ala Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 231
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 231
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtagccaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 232
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 232
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ala 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 233
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 233
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcgg cgatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggtgctgagg aagcagcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 234
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 234
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ala 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Ala Glu Glu Ala Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 235
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 235
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcagccaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcagcttc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 236
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 236
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ala 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Ala Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 237
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 237
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 238
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 238
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 239
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 239
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcgg cggcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcagcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 240
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 240
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ala 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 241
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 241
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcgcggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 242
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 242
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 243
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 243
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 244
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 244
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 245
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 245
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcgg cgatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 246
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 246
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ala 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 247
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 247
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggtgctgagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 248
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 248
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Ala Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 249
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 249
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggcaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgct ccgactgcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatggcttc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cggggcaacc gcacag                           756

 
<210> 250
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 250
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Ala Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Thr Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Ala Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Ala Thr Ala Gln                 
                245                 250         

 
<210> 251
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 251
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 252
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 252
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 253
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 253
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 254
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 254
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 255
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 255
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaagctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cgtactcgca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat tgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 256
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 256
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Arg Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 257
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 257
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtg ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 258
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 258
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 259
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 259
atgaccgatc gtctgaagca gaaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaagctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat tgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 260
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 260
Met Thr Asp Arg Leu Lys Gln Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 261
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 261
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 262
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 262
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 263
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 263
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaaatc ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat tgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 264
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 264
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Ile Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 265
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 265
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgagcgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat tgtaggcgct ccgatggcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 266
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 266
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 267
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 267
atgaccgacc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgagcgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
tatactcaga cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccc cacggaagga tcagctggaa 600
ggttgggagg aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 268
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 268
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Tyr Thr Gln Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Pro Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 269
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 269
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 270
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 270
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 271
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 271
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 272
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 272
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 273
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 273
atgaccgacc gtctgaagca caaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 274
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 274
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 275
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 275
atgaccgacc gtctgaagca gaaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgat ccgacgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 276
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 276
Met Thr Asp Arg Leu Lys Gln Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 277
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 277
atgaccgatc gtctgaagca caaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgtcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat ggtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 278
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 278
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Met Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 279
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 279
atgaccgatc gtctgaagca taaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtgag 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 280
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 280
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg Glu Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 281
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 281
atgaccgacc gtctgaagca caaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgtcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacga cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgat ccgactctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 282
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 282
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Thr Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 283
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 283
atgaccgacc gtctgaagca gaaagtagcc atcgttaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggcggca ctgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaaactgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggacatggat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg aaatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 284
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 284
Met Thr Asp Arg Leu Lys Gln Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Thr Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Met Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 285
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 285
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggcggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 286
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 286
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 287
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 287
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccgtcggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 288
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 288
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ser Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 289
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 289
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagacct cggctaaaga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 290
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 290
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Ser Ala Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 291
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 291
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagccgagg gcgtcattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggcag gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 292
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 292
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Ala Glu Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 293
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 293
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgat ccggcgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 294
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 294
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Ala Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 295
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 295
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggtgatg gccgtattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 296
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 296
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Asp Gly Arg Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 297
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 297
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggtgctg gcaaaattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtcgtgtt ctgtccgtga atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 298
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 298
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Lys Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Arg Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 299
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 299
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggtgctg gccgtattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtga cgctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 300
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 300
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Arg Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Thr Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 301
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 301
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggtcgtg gcgccattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtga cgctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 302
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 302
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Arg Gly Ala Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Thr Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 303
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 303
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggatgg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 304
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 304
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Met Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 305
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 305
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggtgagg gcgtcattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtcgtgtt ctgtccgtgg cgctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 306
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 306
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Glu Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Arg Val Leu Ser 
            100                 105                 110         
Val Ala Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 307
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 307
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtgg cgctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 308
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 308
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Ala Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 309
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 309
atgaccgacc gtctgaagca caaagtagcc atcattaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtcggaaga agcaggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagctgatg gccgtattaa aagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct cgtaggcgat ccgatgctgg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aacggaagga tcagctggaa 600
ggttgggagg agatgatgtc acagcgtacg ctgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcgtctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 310
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 310
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Ala Asp Gly Arg Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Asp Pro Met Leu Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Glu Met Met Ser Gln 
        195                 200                 205             
Arg Thr Leu Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 311
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 311
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtggccaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggcggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 312
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 312
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ala 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 313
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 313
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtgatcaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagctcga tcgcagggct ggtaggcgcc ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtggacc gcacag                           756

 
<210> 314
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 314
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Trp Thr Ala Gln                 
                245                 250         

 
<210> 315
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 315
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagcctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 316
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 316
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 317
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 317
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 318
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 318
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 319
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 319
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aagcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 320
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 320
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 321
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 321
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 322
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 322
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 323
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 323
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagcctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggggctg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 324
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 324
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 325
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 325
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtctgaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgagtctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 326
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 326
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Leu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 327
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 327
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 328
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 328
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 329
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 329
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 330
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 330
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 331
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 331
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcggaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 332
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 332
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 333
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 333
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 334
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 334
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 335
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 335
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aggcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 336
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 336
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ala Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 337
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 337
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggctat ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 338
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 338
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Tyr Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 339
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 339
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct gccgccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 340
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 340
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Ala Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 341
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 341
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 342
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 342
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 343
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 343
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggatcg ggcgtattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 344
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 344
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Arg Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 345
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 345
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggggcag gggtcattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 346
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 346
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 347
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 347
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggggcag ggcgtattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttgctgggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 348
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 348
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Arg Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 349
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 349
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggggcag gggtcattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaagtt ctgtccgtca ctctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttgctgggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 350
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 350
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Thr Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 351
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 351
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttgctgggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aggcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 352
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 352
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Ala Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ala Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 353
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 353
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggatcg gggtcattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 354
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 354
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 355
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 355
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaagctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 356
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 356
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 357
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 357
atgaccgatc gtctgaagca caaagtagcc atcgtcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgtcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaggtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 358
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 358
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Val Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 359
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 359
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgtcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaggtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgatggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 360
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 360
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Met Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 361
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 361
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgtcgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtaaggtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 362
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 362
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Ser Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Lys Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 363
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 363
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgaaaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaagctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agcgcaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 364
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 364
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Lys Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Arg Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 365
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 365
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcaggggccg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 366
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 366
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ala Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 367
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 367
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gttcgtgtca acacagtaca tccgggcgcg atcaagacca actcgaagga tcagctggag 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 368
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 368
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Asn Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 369
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 369
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gttcgtgtca acacagtaca tccgggcgcg atcaagacca actcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 370
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 370
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Asn Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 371
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 371
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 372
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 372
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 373
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 373
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggcggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 374
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 374
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Ala Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 375
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 375
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttccgaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagacca actcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 376
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 376
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Pro 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Asn Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 377
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 377
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttccgaa gagcgttgaa 300
cggactacca cggaggaatg gagtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat cgtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 378
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 378
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Pro 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Ser Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 379
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 379
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atccgcaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 380
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 380
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Arg 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 381
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 381
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat tgtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 382
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 382
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 383
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 383
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cgcactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat cgtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 384
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 384
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 385
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 385
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccgtgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctggag 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 386
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 386
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Val Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 387
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 387
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccgtgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggttatcaa gagcgttgaa 300
cgcactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggat cgtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctggag 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 388
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 388
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Val Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Ile Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 389
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 389
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacagc cggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atcaggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 390
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 390
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Ala Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Gln Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 391
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 391
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacagc cggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccgtgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg aatcgaagga tcagctgcca 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 392
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 392
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Ala Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Val Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Pro Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 393
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 393
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tggcctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 394
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 394
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gly Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 395
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 395
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcaggctgg acgaccctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcgcctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 396
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 396
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Arg Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 397
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 397
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga acgtggctgg acggacctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
caaactacta cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgcaggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc agctgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 398
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 398
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Arg Gly Trp Thr Asp Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Gln Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Gln Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Leu Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 399
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 399
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agagggctgg acggacctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
aagactacta cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgcaggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc agctgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 400
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 400
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Glu Gly Trp Thr Asp Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Lys Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Gln Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Leu Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 401
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 401
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcgggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
atgactacta cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc agctgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 402
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 402
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Met Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Leu Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 403
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 403
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga acgtggctgg acgcgcctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtg agggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 404
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 404
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Arg Gly Trp Thr Arg Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Glu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 405
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 405
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga acgtggctgg acgcgcctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cgcactacta cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtg agggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgcaggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 406
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 406
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Arg Gly Trp Thr Arg Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Glu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Gln Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 407
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 407
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga agcgggctgg acggacctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtc tgggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgcaggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc aggcaatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 408
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 408
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Ala Gly Trp Thr Asp Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Leu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Gln Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Ala Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 409
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 409
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga acgtggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
cggactacca cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtg agggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccggccgccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc agctgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 410
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 410
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Arg Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Arg Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Glu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Ala Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Leu Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         

 
<210> 411
<211> 756
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 411
atgaccgatc gtctgaagca caaagtagcc atcatcaccg gcgggacaat gggtatcggt 60
ttggcaatcg ccgataaatt tgtagaggag ggtgcgaaag tagttattac tggtcgtcac 120
gcggatgtag gtgagaaagc cgccaaatca atcggtggca gtgatgttat tcgctttgtc 180
cagcacgatg cgtccgaaga acgtggctgg acgacgctgt tcgacaccac cgaggaggca 240
ttcggcccgg ttacgaccct ggtgaacaat gcagggattg gggtaattaa gagcgttgaa 300
caaactacta cggaggaatg gcgtgatgtt ctgtccgtta atctggatgg tgtttttttc 360
ggcacccgtg agggcattca gcgcatgaaa aataaaggct tgggcgctag catcatcaat 420
atgagcagta ttatggggct ggtaggcgct ccgcaggccg gggcatacaa cgcttccaag 480
ggggcggtac gtatcatgtc gaaaagcgca gcgctggatt gcgcactgaa ggactacgat 540
gtgcgtgtca acacagtaca tccgggcgcg atcaagaccg agtcgaagga tcagctggaa 600
ggttgggagc agctgatgtc acagcgtacg gcgaccccta tgggccacat tggcgaaccg 660
aatgacatcg catggatctg tgtgtacctg gcatctgacg aatcgaaatt tgcgacgggt 720
gcagaatttg tggtcgacgg cgggtacacc gcacag                           756

 
<210> 412
<211> 252
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Engineered Variant of Kred from Lactobacillus kefir
 
<400> 412
Met Thr Asp Arg Leu Lys His Lys Val Ala Ile Ile Thr Gly Gly Thr 
1               5                   10                  15      
Met Gly Ile Gly Leu Ala Ile Ala Asp Lys Phe Val Glu Glu Gly Ala 
            20                  25                  30          
Lys Val Val Ile Thr Gly Arg His Ala Asp Val Gly Glu Lys Ala Ala 
        35                  40                  45              
Lys Ser Ile Gly Gly Ser Asp Val Ile Arg Phe Val Gln His Asp Ala 
    50                  55                  60                  
Ser Glu Glu Arg Gly Trp Thr Thr Leu Phe Asp Thr Thr Glu Glu Ala 
65                  70                  75                  80  
Phe Gly Pro Val Thr Thr Leu Val Asn Asn Ala Gly Ile Gly Val Ile 
                85                  90                  95      
Lys Ser Val Glu Gln Thr Thr Thr Glu Glu Trp Arg Asp Val Leu Ser 
            100                 105                 110         
Val Asn Leu Asp Gly Val Phe Phe Gly Thr Arg Glu Gly Ile Gln Arg 
        115                 120                 125             
Met Lys Asn Lys Gly Leu Gly Ala Ser Ile Ile Asn Met Ser Ser Ile 
    130                 135                 140                 
Met Gly Leu Val Gly Ala Pro Gln Ala Gly Ala Tyr Asn Ala Ser Lys 
145                 150                 155                 160 
Gly Ala Val Arg Ile Met Ser Lys Ser Ala Ala Leu Asp Cys Ala Leu 
                165                 170                 175     
Lys Asp Tyr Asp Val Arg Val Asn Thr Val His Pro Gly Ala Ile Lys 
            180                 185                 190         
Thr Glu Ser Lys Asp Gln Leu Glu Gly Trp Glu Gln Leu Met Ser Gln 
        195                 200                 205             
Arg Thr Ala Thr Pro Met Gly His Ile Gly Glu Pro Asn Asp Ile Ala 
    210                 215                 220                 
Trp Ile Cys Val Tyr Leu Ala Ser Asp Glu Ser Lys Phe Ala Thr Gly 
225                 230                 235                 240 
Ala Glu Phe Val Val Asp Gly Gly Tyr Thr Ala Gln                 
                245                 250         


