               SEQUENCE LISTING

<110> CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC)

<120> Xylanase enzyme with extreme thermostability and alkaline stability

<130> PCT1641.1555

<150> EP20382849
<151> 2020-09-25

<160> 20

<170> BiSSAP 1.3.6

<210> 1
<211> 343
<212> PRT
<213> Pseudothermotoga thermarum


<400> 1
Gly Pro Gln Pro Met Ser Ser Gln Ile Pro Ser Leu Lys Asp Val Phe 
1               5                   10                  15      
Leu Gln Asp Phe Lys Ile Gly Val Ala Leu Pro Val Arg Val Phe Ser 
            20                  25                  30          
Asn Ser Met Asp Val Glu Leu Ile Thr Lys His Phe Asn Ser Met Thr 
        35                  40                  45              
Ala Glu Asn Glu Met Lys Pro Glu Ser Ile Leu Arg Arg Asp Ala Ser 
    50                  55                  60                  
Gly Lys Ile Tyr Tyr Asp Phe Thr Val Ala Asp Arg Tyr Ile Glu Phe 
65                  70                  75                  80  
Ala Gln Lys His Gly Met Val Val Arg Gly His Thr Leu Val Trp His 
                85                  90                  95      
Ser Gln Thr Pro Glu Trp Phe Phe Lys Asp Glu Lys Gly Asn Leu Leu 
            100                 105                 110         
Ser Arg Glu Ala Met Ile Glu Arg Met Arg Glu Tyr Ile His Thr Val 
        115                 120                 125             
Val Gly Arg Tyr Arg Gly Lys Val Tyr Ala Trp Asp Val Val Asn Glu 
    130                 135                 140                 
Ala Val Asp Glu Asn Gln Pro Asp Gly Leu Arg Arg Ser Leu Trp Tyr 
145                 150                 155                 160 
Gln Val Ile Gly Pro Asp Tyr Ile Glu Leu Ala Phe Lys Phe Ala His 
                165                 170                 175     
Glu Ala Asp Pro Asp Ala Leu Leu Phe Tyr Asn Asp Tyr Asn Glu Phe 
            180                 185                 190         
Phe Pro Lys Lys Arg Asp Ile Ile Phe Lys Leu Val Lys Glu Met Arg 
        195                 200                 205             
Glu Lys Gly Val Pro Ile His Gly Ile Gly Met Gln Gln His Leu Thr 
    210                 215                 220                 
Leu Ala Asp Asn Val Gly Trp Ile Asp Ile Ala Ile Gln Lys Phe Lys 
225                 230                 235                 240 
Thr Ile Ser Gly Ile Gln Ile His Ile Thr Glu Leu Asp Val Ser Val 
                245                 250                 255     
Tyr Lys Ser Arg Ser Pro Ser Ile Ile Tyr Gln Thr Pro Pro Leu Glu 
            260                 265                 270         
Val Leu Lys Glu Gln Ala Glu Phe Tyr Arg Lys Leu Phe Glu Ile Tyr 
        275                 280                 285             
Arg Lys His Thr Asp Val Ile Thr Asn Val Thr Phe Trp Gly Leu Lys 
    290                 295                 300                 
Asp Asp Tyr Ser Trp Leu Arg Phe Phe Phe Gly Arg Arg Asn Asp Trp 
305                 310                 315                 320 
Pro Leu Leu Phe Asp Glu Asn Tyr Gln Pro Lys Pro Ala Phe Trp Ser 
                325                 330                 335     
Val Ile Glu Ser Val Ser Lys 
            340             

<210> 2
<211> 21
<212> PRT
<213> Pseudothermotoga thermarum


<400> 2
Met Lys Lys Leu Ile Phe Ala Ile Thr Leu Leu Leu Ile Val Phe Thr 
1               5                   10                  15      
Val Ile Ile Lys Gly 
            20      

<210> 3
<211> 364
<212> PRT
<213> Pseudothermotoga thermarum


<400> 3
Met Lys Lys Leu Ile Phe Ala Ile Thr Leu Leu Leu Ile Val Phe Thr 
1               5                   10                  15      
Val Ile Ile Lys Gly Gly Pro Gln Pro Met Ser Ser Gln Ile Pro Ser 
            20                  25                  30          
Leu Lys Asp Val Phe Leu Gln Asp Phe Lys Ile Gly Val Ala Leu Pro 
        35                  40                  45              
Val Arg Val Phe Ser Asn Ser Met Asp Val Glu Leu Ile Thr Lys His 
    50                  55                  60                  
Phe Asn Ser Met Thr Ala Glu Asn Glu Met Lys Pro Glu Ser Ile Leu 
65                  70                  75                  80  
Arg Arg Asp Ala Ser Gly Lys Ile Tyr Tyr Asp Phe Thr Val Ala Asp 
                85                  90                  95      
Arg Tyr Ile Glu Phe Ala Gln Lys His Gly Met Val Val Arg Gly His 
            100                 105                 110         
Thr Leu Val Trp His Ser Gln Thr Pro Glu Trp Phe Phe Lys Asp Glu 
        115                 120                 125             
Lys Gly Asn Leu Leu Ser Arg Glu Ala Met Ile Glu Arg Met Arg Glu 
    130                 135                 140                 
Tyr Ile His Thr Val Val Gly Arg Tyr Arg Gly Lys Val Tyr Ala Trp 
145                 150                 155                 160 
Asp Val Val Asn Glu Ala Val Asp Glu Asn Gln Pro Asp Gly Leu Arg 
                165                 170                 175     
Arg Ser Leu Trp Tyr Gln Val Ile Gly Pro Asp Tyr Ile Glu Leu Ala 
            180                 185                 190         
Phe Lys Phe Ala His Glu Ala Asp Pro Asp Ala Leu Leu Phe Tyr Asn 
        195                 200                 205             
Asp Tyr Asn Glu Phe Phe Pro Lys Lys Arg Asp Ile Ile Phe Lys Leu 
    210                 215                 220                 
Val Lys Glu Met Arg Glu Lys Gly Val Pro Ile His Gly Ile Gly Met 
225                 230                 235                 240 
Gln Gln His Leu Thr Leu Ala Asp Asn Val Gly Trp Ile Asp Ile Ala 
                245                 250                 255     
Ile Gln Lys Phe Lys Thr Ile Ser Gly Ile Gln Ile His Ile Thr Glu 
            260                 265                 270         
Leu Asp Val Ser Val Tyr Lys Ser Arg Ser Pro Ser Ile Ile Tyr Gln 
        275                 280                 285             
Thr Pro Pro Leu Glu Val Leu Lys Glu Gln Ala Glu Phe Tyr Arg Lys 
    290                 295                 300                 
Leu Phe Glu Ile Tyr Arg Lys His Thr Asp Val Ile Thr Asn Val Thr 
305                 310                 315                 320 
Phe Trp Gly Leu Lys Asp Asp Tyr Ser Trp Leu Arg Phe Phe Phe Gly 
                325                 330                 335     
Arg Arg Asn Asp Trp Pro Leu Leu Phe Asp Glu Asn Tyr Gln Pro Lys 
            340                 345                 350         
Pro Ala Phe Trp Ser Val Ile Glu Ser Val Ser Lys 
        355                 360                 

<210> 4
<211> 104
<212> PRT
<213> Artificial Sequence


<220> 
<223> CBM2 domian

<400> 4
Thr Thr Thr Pro Val Pro Val Ser Gly Ser Leu Glu Val Lys Val Asn 
1               5                   10                  15      
Asp Trp Gly Ser Gly Ala Glu Tyr Asp Val Thr Leu Asn Leu Asp Gly 
            20                  25                  30          
Gln Tyr Asp Trp Thr Val Lys Val Lys Leu Ala Pro Gly Ala Thr Val 
        35                  40                  45              
Gly Ser Phe Trp Ser Ala Asn Lys Gln Glu Gly Asn Gly Tyr Val Ile 
    50                  55                  60                  
Phe Thr Pro Val Ser Trp Asn Lys Gly Pro Thr Ala Thr Phe Gly Phe 
65                  70                  75                  80  
Ile Val Asn Gly Pro Gln Gly Asp Lys Val Glu Glu Ile Thr Leu Glu 
                85                  90                  95      
Ile Asn Gly Gln Val Ile Glu Ala 
            100                 

<210> 5
<211> 191
<212> PRT
<213> Artificial Sequence


<220> 
<223> CBM9 domain

<400> 5
Gly Ile Met Val Ala Thr Ala Lys Tyr Gly Thr Pro Val Ile Asp Gly 
1               5                   10                  15      
Glu Ile Asp Glu Ile Trp Asn Thr Thr Glu Glu Ile Glu Thr Lys Ala 
            20                  25                  30          
Val Ala Met Gly Ser Leu Asp Lys Asn Ala Thr Ala Lys Val Arg Val 
        35                  40                  45              
Leu Trp Asp Glu Asn Tyr Leu Tyr Val Leu Ala Ile Val Lys Asp Pro 
    50                  55                  60                  
Val Leu Asn Lys Asp Asn Ser Asn Pro Trp Glu Gln Asp Ser Val Glu 
65                  70                  75                  80  
Ile Phe Ile Asp Glu Asn Asn His Lys Thr Gly Tyr Tyr Glu Asp Asp 
                85                  90                  95      
Asp Ala Gln Phe Arg Val Asn Tyr Met Asn Glu Gln Thr Phe Gly Thr 
            100                 105                 110         
Gly Gly Ser Pro Ala Arg Phe Lys Thr Ala Val Lys Leu Ile Glu Gly 
        115                 120                 125             
Gly Tyr Ile Val Glu Ala Ala Ile Lys Trp Lys Thr Ile Lys Pro Thr 
    130                 135                 140                 
Pro Asn Thr Val Ile Gly Phe Asn Ile Gln Val Asn Asp Ala Asn Glu 
145                 150                 155                 160 
Lys Gly Gln Arg Val Gly Ile Ile Ser Trp Ser Asp Pro Thr Asn Asn 
                165                 170                 175     
Ser Trp Arg Asp Pro Ser Lys Phe Gly Asn Leu Arg Leu Ile Lys 
            180                 185                 190     

<210> 6
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker peptide

<400> 6
Val Asp Gly Ala 
1               

<210> 7
<211> 451
<212> PRT
<213> Artificial Sequence


<220> 
<223> X11 with CBM2 domain

<400> 7
Gly Pro Gln Pro Met Ser Ser Gln Ile Pro Ser Leu Lys Asp Val Phe 
1               5                   10                  15      
Leu Gln Asp Phe Lys Ile Gly Val Ala Leu Pro Val Arg Val Phe Ser 
            20                  25                  30          
Asn Ser Met Asp Val Glu Leu Ile Thr Lys His Phe Asn Ser Met Thr 
        35                  40                  45              
Ala Glu Asn Glu Met Lys Pro Glu Ser Ile Leu Arg Arg Asp Ala Ser 
    50                  55                  60                  
Gly Lys Ile Tyr Tyr Asp Phe Thr Val Ala Asp Arg Tyr Ile Glu Phe 
65                  70                  75                  80  
Ala Gln Lys His Gly Met Val Val Arg Gly His Thr Leu Val Trp His 
                85                  90                  95      
Ser Gln Thr Pro Glu Trp Phe Phe Lys Asp Glu Lys Gly Asn Leu Leu 
            100                 105                 110         
Ser Arg Glu Ala Met Ile Glu Arg Met Arg Glu Tyr Ile His Thr Val 
        115                 120                 125             
Val Gly Arg Tyr Arg Gly Lys Val Tyr Ala Trp Asp Val Val Asn Glu 
    130                 135                 140                 
Ala Val Asp Glu Asn Gln Pro Asp Gly Leu Arg Arg Ser Leu Trp Tyr 
145                 150                 155                 160 
Gln Val Ile Gly Pro Asp Tyr Ile Glu Leu Ala Phe Lys Phe Ala His 
                165                 170                 175     
Glu Ala Asp Pro Asp Ala Leu Leu Phe Tyr Asn Asp Tyr Asn Glu Phe 
            180                 185                 190         
Phe Pro Lys Lys Arg Asp Ile Ile Phe Lys Leu Val Lys Glu Met Arg 
        195                 200                 205             
Glu Lys Gly Val Pro Ile His Gly Ile Gly Met Gln Gln His Leu Thr 
    210                 215                 220                 
Leu Ala Asp Asn Val Gly Trp Ile Asp Ile Ala Ile Gln Lys Phe Lys 
225                 230                 235                 240 
Thr Ile Ser Gly Ile Gln Ile His Ile Thr Glu Leu Asp Val Ser Val 
                245                 250                 255     
Tyr Lys Ser Arg Ser Pro Ser Ile Ile Tyr Gln Thr Pro Pro Leu Glu 
            260                 265                 270         
Val Leu Lys Glu Gln Ala Glu Phe Tyr Arg Lys Leu Phe Glu Ile Tyr 
        275                 280                 285             
Arg Lys His Thr Asp Val Ile Thr Asn Val Thr Phe Trp Gly Leu Lys 
    290                 295                 300                 
Asp Asp Tyr Ser Trp Leu Arg Phe Phe Phe Gly Arg Arg Asn Asp Trp 
305                 310                 315                 320 
Pro Leu Leu Phe Asp Glu Asn Tyr Gln Pro Lys Pro Ala Phe Trp Ser 
                325                 330                 335     
Val Ile Glu Ser Val Ser Lys Val Asp Gly Ala Thr Thr Thr Pro Val 
            340                 345                 350         
Pro Val Ser Gly Ser Leu Glu Val Lys Val Asn Asp Trp Gly Ser Gly 
        355                 360                 365             
Ala Glu Tyr Asp Val Thr Leu Asn Leu Asp Gly Gln Tyr Asp Trp Thr 
    370                 375                 380                 
Val Lys Val Lys Leu Ala Pro Gly Ala Thr Val Gly Ser Phe Trp Ser 
385                 390                 395                 400 
Ala Asn Lys Gln Glu Gly Asn Gly Tyr Val Ile Phe Thr Pro Val Ser 
                405                 410                 415     
Trp Asn Lys Gly Pro Thr Ala Thr Phe Gly Phe Ile Val Asn Gly Pro 
            420                 425                 430         
Gln Gly Asp Lys Val Glu Glu Ile Thr Leu Glu Ile Asn Gly Gln Val 
        435                 440                 445             
Ile Glu Ala 
    450     

<210> 8
<211> 538
<212> PRT
<213> Artificial Sequence


<220> 
<223> X11 with CBM9 domain

<400> 8
Gly Pro Gln Pro Met Ser Ser Gln Ile Pro Ser Leu Lys Asp Val Phe 
1               5                   10                  15      
Leu Gln Asp Phe Lys Ile Gly Val Ala Leu Pro Val Arg Val Phe Ser 
            20                  25                  30          
Asn Ser Met Asp Val Glu Leu Ile Thr Lys His Phe Asn Ser Met Thr 
        35                  40                  45              
Ala Glu Asn Glu Met Lys Pro Glu Ser Ile Leu Arg Arg Asp Ala Ser 
    50                  55                  60                  
Gly Lys Ile Tyr Tyr Asp Phe Thr Val Ala Asp Arg Tyr Ile Glu Phe 
65                  70                  75                  80  
Ala Gln Lys His Gly Met Val Val Arg Gly His Thr Leu Val Trp His 
                85                  90                  95      
Ser Gln Thr Pro Glu Trp Phe Phe Lys Asp Glu Lys Gly Asn Leu Leu 
            100                 105                 110         
Ser Arg Glu Ala Met Ile Glu Arg Met Arg Glu Tyr Ile His Thr Val 
        115                 120                 125             
Val Gly Arg Tyr Arg Gly Lys Val Tyr Ala Trp Asp Val Val Asn Glu 
    130                 135                 140                 
Ala Val Asp Glu Asn Gln Pro Asp Gly Leu Arg Arg Ser Leu Trp Tyr 
145                 150                 155                 160 
Gln Val Ile Gly Pro Asp Tyr Ile Glu Leu Ala Phe Lys Phe Ala His 
                165                 170                 175     
Glu Ala Asp Pro Asp Ala Leu Leu Phe Tyr Asn Asp Tyr Asn Glu Phe 
            180                 185                 190         
Phe Pro Lys Lys Arg Asp Ile Ile Phe Lys Leu Val Lys Glu Met Arg 
        195                 200                 205             
Glu Lys Gly Val Pro Ile His Gly Ile Gly Met Gln Gln His Leu Thr 
    210                 215                 220                 
Leu Ala Asp Asn Val Gly Trp Ile Asp Ile Ala Ile Gln Lys Phe Lys 
225                 230                 235                 240 
Thr Ile Ser Gly Ile Gln Ile His Ile Thr Glu Leu Asp Val Ser Val 
                245                 250                 255     
Tyr Lys Ser Arg Ser Pro Ser Ile Ile Tyr Gln Thr Pro Pro Leu Glu 
            260                 265                 270         
Val Leu Lys Glu Gln Ala Glu Phe Tyr Arg Lys Leu Phe Glu Ile Tyr 
        275                 280                 285             
Arg Lys His Thr Asp Val Ile Thr Asn Val Thr Phe Trp Gly Leu Lys 
    290                 295                 300                 
Asp Asp Tyr Ser Trp Leu Arg Phe Phe Phe Gly Arg Arg Asn Asp Trp 
305                 310                 315                 320 
Pro Leu Leu Phe Asp Glu Asn Tyr Gln Pro Lys Pro Ala Phe Trp Ser 
                325                 330                 335     
Val Ile Glu Ser Val Ser Lys Val Asp Gly Ala Gly Ile Met Val Ala 
            340                 345                 350         
Thr Ala Lys Tyr Gly Thr Pro Val Ile Asp Gly Glu Ile Asp Glu Ile 
        355                 360                 365             
Trp Asn Thr Thr Glu Glu Ile Glu Thr Lys Ala Val Ala Met Gly Ser 
    370                 375                 380                 
Leu Asp Lys Asn Ala Thr Ala Lys Val Arg Val Leu Trp Asp Glu Asn 
385                 390                 395                 400 
Tyr Leu Tyr Val Leu Ala Ile Val Lys Asp Pro Val Leu Asn Lys Asp 
                405                 410                 415     
Asn Ser Asn Pro Trp Glu Gln Asp Ser Val Glu Ile Phe Ile Asp Glu 
            420                 425                 430         
Asn Asn His Lys Thr Gly Tyr Tyr Glu Asp Asp Asp Ala Gln Phe Arg 
        435                 440                 445             
Val Asn Tyr Met Asn Glu Gln Thr Phe Gly Thr Gly Gly Ser Pro Ala 
    450                 455                 460                 
Arg Phe Lys Thr Ala Val Lys Leu Ile Glu Gly Gly Tyr Ile Val Glu 
465                 470                 475                 480 
Ala Ala Ile Lys Trp Lys Thr Ile Lys Pro Thr Pro Asn Thr Val Ile 
                485                 490                 495     
Gly Phe Asn Ile Gln Val Asn Asp Ala Asn Glu Lys Gly Gln Arg Val 
            500                 505                 510         
Gly Ile Ile Ser Trp Ser Asp Pro Thr Asn Asn Ser Trp Arg Asp Pro 
        515                 520                 525             
Ser Lys Phe Gly Asn Leu Arg Leu Ile Lys 
    530                 535             

<210> 9
<211> 1032
<212> DNA
<213> Pseudothermotoga thermarum


<400> 9
ggaccacagc ccatgtcgtc acagatacca tcccttaagg atgttttttt gcaggatttc      60

aaaataggag tagctttacc agttagagtt ttcagcaatt cgatggatgt ggagttaatt     120

acgaagcact ttaacagtat gacggctgag aacgagatga aaccagagag tatattgaga     180

agggatgcga gtgggaagat ttactatgat tttacggttg cagacaggta catagagttt     240

gcgcagaaac atgggatggt tgttaggggt catacacttg tttggcacag tcagacgccg     300

gagtggtttt tcaaggatga gaagggcaat ttattgagca gggaagcgat gatagagagg     360

atgcgggagt acatacacac agttgtaggc aggtatagag gcaaggtgta tgcgtgggat     420

gtggtgaacg aggcggtgga tgagaatcag cctgatggtt tgaggaggag tttgtggtat     480

caggttatag ggccggatta catagagctt gccttcaaat ttgctcacga agcagatcca     540

gacgccttac tcttctacaa cgattacaac gaatttttcc ccaaaaagcg agacatcatc     600

ttcaaattgg taaaagaaat gagagaaaag ggtgtaccga ttcatggtat tggcatgcag     660

caacatttaa ctcttgcaga taacgtaggt tggattgata ttgctattca aaaattcaag     720

accatttcag gtatacaaat tcacataact gagcttgatg ttagtgttta caaatcacgc     780

agtccatcta ttatctatca aacgccaccc ttggaggtgc tcaaggagca agcggaattt     840

taccgcaagt tgttcgaaat ctataggaaa cacactgacg tcataaccaa cgttaccttc     900

tggggtttga aagatgacta ttcgtggcta aggtttttct ttggtcgtag aaacgattgg     960

ccgcttttat ttgacgaaaa ttatcaaccc aaaccagctt tttggagtgt aatagaatca    1020

gtttccaagt aa                                                        1032


<210> 10
<211> 63
<212> DNA
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 10
atgaaaaagc tgattttcgc catcacattg ttgcttatag tctttactgt gataatcaaa     60

gga                                                                   63


<210> 11
<211> 12
<212> DNA
<213> Artificial Sequence


<220> 
<223> Linker

<400> 11
gtcgacggcg ca                                                        12


<210> 12
<211> 1029
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11 optimized

<400> 12
gggcctcagc cgatgtcgtc gcaaatcccc tcactgaagg atgtctttct tcaagatttc      60

aaaatcggcg tggcactgcc ggtacgcgtc ttctctaatt ccatggatgt tgagttaatt     120

accaaacatt tcaattcgat gacagcggaa aatgagatga agcccgaatc gattttacgt     180

cgtgatgcct cggggaagat ttattatgac ttcaccgttg ctgaccgcta catcgaattt     240

gcacagaagc atggcatggt ggtacgcgga cacacattag tttggcactc ccaaacaccg     300

gaatggtttt tcaaagatga aaagggcaat ttattgtcgc gtgaagcaat gattgaacgt     360

atgcgcgaat acatccacac agtagtaggc cgctaccgtg ggaaagttta cgcttgggac     420

gtcgttaatg aagccgtgga cgaaaaccag ccagacggac tgcgtcgttc gttatggtat     480

caggttatcg gcccagatta tattgagctt gcatttaagt ttgcgcatga ggctgaccca     540

gatgcattgt tattttacaa cgactataat gaattctttc cgaaaaaacg tgacatcatc     600

tttaagttgg ttaaggagat gcgcgaaaag ggcgtcccaa ttcatgggat cggaatgcaa     660

cagcatctta cacttgcgga caacgtgggg tggatcgata tcgcaatcca aaaatttaag     720

actatcagtg ggatccaaat ccatattacg gaacttgacg tatctgtcta caagagtcgt     780

tctccaagca ttatttatca gacacctcca cttgaagtgc ttaaagaaca agcggaattt     840

tatcgtaaac tttttgagat ttatcgcaag cacaccgacg taattaccaa tgttacattt     900

tgggggttga aggacgacta cagctggttg cgtttctttt ttggccgtcg caacgactgg     960

ccgctgcttt ttgacgagaa ttatcaaccg aaacctgcgt tttggagtgt tatcgaatca    1020

gtaagcaag                                                            1029


<210> 13
<211> 1356
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11opt with CBM2 domain

<400> 13
gggcctcagc cgatgtcgtc gcaaatcccc tcactgaagg atgtctttct tcaagatttc      60

aaaatcggcg tggcactgcc ggtacgcgtc ttctctaatt ccatggatgt tgagttaatt     120

accaaacatt tcaattcgat gacagcggaa aatgagatga agcccgaatc gattttacgt     180

cgtgatgcct cggggaagat ttattatgac ttcaccgttg ctgaccgcta catcgaattt     240

gcacagaagc atggcatggt ggtacgcgga cacacattag tttggcactc ccaaacaccg     300

gaatggtttt tcaaagatga aaagggcaat ttattgtcgc gtgaagcaat gattgaacgt     360

atgcgcgaat acatccacac agtagtaggc cgctaccgtg ggaaagttta cgcttgggac     420

gtcgttaatg aagccgtgga cgaaaaccag ccagacggac tgcgtcgttc gttatggtat     480

caggttatcg gcccagatta tattgagctt gcatttaagt ttgcgcatga ggctgaccca     540

gatgcattgt tattttacaa cgactataat gaattctttc cgaaaaaacg tgacatcatc     600

tttaagttgg ttaaggagat gcgcgaaaag ggcgtcccaa ttcatgggat cggaatgcaa     660

cagcatctta cacttgcgga caacgtgggg tggatcgata tcgcaatcca aaaatttaag     720

actatcagtg ggatccaaat ccatattacg gaacttgacg tatctgtcta caagagtcgt     780

tctccaagca ttatttatca gacacctcca cttgaagtgc ttaaagaaca agcggaattt     840

tatcgtaaac tttttgagat ttatcgcaag cacaccgacg taattaccaa tgttacattt     900

tgggggttga aggacgacta cagctggttg cgtttctttt ttggccgtcg caacgactgg     960

ccgctgcttt ttgacgagaa ttatcaaccg aaacctgcgt tttggagtgt tatcgaatca    1020

gtaagcaagg tcgacggcgc aacaactacc cctgtcccag tctcaggatc tctagaggta    1080

aaggtaaacg attggggtag tggtgctgag tatgatgtga ctcttaattt ggatggacag    1140

tatgactgga ctgtgaaggt taaactagcc ccaggagcca ctgtaggaag cttctggagc    1200

gctaacaaac aagaggggaa tggctatgtc atcttcactc cagtaagctg gaataaaggg    1260

ccgacagcaa catttggatt catagtaaac ggaccacaag gagacaaagt agaagaaata    1320

accctagaaa taaacggaca agtaattgaa gcttaa                              1356


<210> 14
<211> 1614
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11opt with CBM9 domain

<400> 14
gggcctcagc cgatgtcgtc gcaaatcccc tcactgaagg atgtctttct tcaagatttc      60

aaaatcggcg tggcactgcc ggtacgcgtc ttctctaatt ccatggatgt tgagttaatt     120

accaaacatt tcaattcgat gacagcggaa aatgagatga agcccgaatc gattttacgt     180

cgtgatgcct cggggaagat ttattatgac ttcaccgttg ctgaccgcta catcgaattt     240

gcacagaagc atggcatggt ggtacgcgga cacacattag tttggcactc ccaaacaccg     300

gaatggtttt tcaaagatga aaagggcaat ttattgtcgc gtgaagcaat gattgaacgt     360

atgcgcgaat acatccacac agtagtaggc cgctaccgtg ggaaagttta cgcttgggac     420

gtcgttaatg aagccgtgga cgaaaaccag ccagacggac tgcgtcgttc gttatggtat     480

caggttatcg gcccagatta tattgagctt gcatttaagt ttgcgcatga ggctgaccca     540

gatgcattgt tattttacaa cgactataat gaattctttc cgaaaaaacg tgacatcatc     600

tttaagttgg ttaaggagat gcgcgaaaag ggcgtcccaa ttcatgggat cggaatgcaa     660

cagcatctta cacttgcgga caacgtgggg tggatcgata tcgcaatcca aaaatttaag     720

actatcagtg ggatccaaat ccatattacg gaacttgacg tatctgtcta caagagtcgt     780

tctccaagca ttatttatca gacacctcca cttgaagtgc ttaaagaaca agcggaattt     840

tatcgtaaac tttttgagat ttatcgcaag cacaccgacg taattaccaa tgttacattt     900

tgggggttga aggacgacta cagctggttg cgtttctttt ttggccgtcg caacgactgg     960

ccgctgcttt ttgacgagaa ttatcaaccg aaacctgcgt tttggagtgt tatcgaatca    1020

gtaagcaagg tcgacggcgc aggaataatg gtagcgacag caaaatacgg aacaccggtc    1080

atcgatggag agatagacga gatctggaac acgacagagg agatagagac gaaagcggtg    1140

gccatgggat cgcttgacaa gaacgcgaca gcgaaagtga gggtgctgtg ggacgagaac    1200

tacctgtacg tacttgcaat cgtgaaagac cccgttctga acaaagacaa cagcaacccg    1260

tgggaacagg attccgtgga gatcttcatc gacgagaaca accacaagac aggatactac    1320

gaagacgacg acgcacagtt cagggtgaac tacatgaacg agcagacgtt tggaacggga    1380

ggaagtccag cgaggttcaa gacagcggtg aaactgatcg aaggaggata catagttgag    1440

gcagcgatca agtggaagac gatcaaaccc acaccgaaca cggtgatagg attcaacatc    1500

caggtgaacg atgcgaacga gaaagggcag agggtcggta tcatctcctg gagcgatccc    1560

acaaacaaca gctggagaga tccttcaaag ttcggtaacc tcaggctcat caag          1614


<210> 15
<211> 1095
<212> DNA
<213> Pseudothermotoga thermarum


<400> 15
atgaaaaagc tgattttcgc catcacattg ttgcttatag tctttactgt gataatcaaa      60

ggaggaccac agcccatgtc gtcacagata ccatccctta aggatgtttt tttgcaggat     120

ttcaaaatag gagtagcttt accagttaga gttttcagca attcgatgga tgtggagtta     180

attacgaagc actttaacag tatgacggct gagaacgaga tgaaaccaga gagtatattg     240

agaagggatg cgagtgggaa gatttactat gattttacgg ttgcagacag gtacatagag     300

tttgcgcaga aacatgggat ggttgttagg ggtcatacac ttgtttggca cagtcagacg     360

ccggagtggt ttttcaagga tgagaagggc aatttattga gcagggaagc gatgatagag     420

aggatgcggg agtacataca cacagttgta ggcaggtata gaggcaaggt gtatgcgtgg     480

gatgtggtga acgaggcggt ggatgagaat cagcctgatg gtttgaggag gagtttgtgg     540

tatcaggtta tagggccgga ttacatagag cttgccttca aatttgctca cgaagcagat     600

ccagacgcct tactcttcta caacgattac aacgaatttt tccccaaaaa gcgagacatc     660

atcttcaaat tggtaaaaga aatgagagaa aagggtgtac cgattcatgg tattggcatg     720

cagcaacatt taactcttgc agataacgta ggttggattg atattgctat tcaaaaattc     780

aagaccattt caggtataca aattcacata actgagcttg atgttagtgt ttacaaatca     840

cgcagtccat ctattatcta tcaaacgcca cccttggagg tgctcaagga gcaagcggaa     900

ttttaccgca agttgttcga aatctatagg aaacacactg acgtcataac caacgttacc     960

ttctggggtt tgaaagatga ctattcgtgg ctaaggtttt tctttggtcg tagaaacgat    1020

tggccgcttt tatttgacga aaattatcaa cccaaaccag ctttttggag tgtaatagaa    1080

tcagtttcca agtaa                                                     1095


<210> 16
<211> 1092
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11 optimized

<400> 16
atgaaaaagc tgattttcgc catcacattg ttgcttatag tctttactgt gataatcaaa      60

ggagggcctc agccgatgtc gtcgcaaatc ccctcactga aggatgtctt tcttcaagat     120

ttcaaaatcg gcgtggcact gccggtacgc gtcttctcta attccatgga tgttgagtta     180

attaccaaac atttcaattc gatgacagcg gaaaatgaga tgaagcccga atcgatttta     240

cgtcgtgatg cctcggggaa gatttattat gacttcaccg ttgctgaccg ctacatcgaa     300

tttgcacaga agcatggcat ggtggtacgc ggacacacat tagtttggca ctcccaaaca     360

ccggaatggt ttttcaaaga tgaaaagggc aatttattgt cgcgtgaagc aatgattgaa     420

cgtatgcgcg aatacatcca cacagtagta ggccgctacc gtgggaaagt ttacgcttgg     480

gacgtcgtta atgaagccgt ggacgaaaac cagccagacg gactgcgtcg ttcgttatgg     540

tatcaggtta tcggcccaga ttatattgag cttgcattta agtttgcgca tgaggctgac     600

ccagatgcat tgttatttta caacgactat aatgaattct ttccgaaaaa acgtgacatc     660

atctttaagt tggttaagga gatgcgcgaa aagggcgtcc caattcatgg gatcggaatg     720

caacagcatc ttacacttgc ggacaacgtg gggtggatcg atatcgcaat ccaaaaattt     780

aagactatca gtgggatcca aatccatatt acggaacttg acgtatctgt ctacaagagt     840

cgttctccaa gcattattta tcagacacct ccacttgaag tgcttaaaga acaagcggaa     900

ttttatcgta aactttttga gatttatcgc aagcacaccg acgtaattac caatgttaca     960

ttttgggggt tgaaggacga ctacagctgg ttgcgtttct tttttggccg tcgcaacgac    1020

tggccgctgc tttttgacga gaattatcaa ccgaaacctg cgttttggag tgttatcgaa    1080

tcagtaagca ag                                                        1092


<210> 17
<211> 1419
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11 optimized with SBM2

<400> 17
atgaaaaagc tgattttcgc catcacattg ttgcttatag tctttactgt gataatcaaa      60

ggagggcctc agccgatgtc gtcgcaaatc ccctcactga aggatgtctt tcttcaagat     120

ttcaaaatcg gcgtggcact gccggtacgc gtcttctcta attccatgga tgttgagtta     180

attaccaaac atttcaattc gatgacagcg gaaaatgaga tgaagcccga atcgatttta     240

cgtcgtgatg cctcggggaa gatttattat gacttcaccg ttgctgaccg ctacatcgaa     300

tttgcacaga agcatggcat ggtggtacgc ggacacacat tagtttggca ctcccaaaca     360

ccggaatggt ttttcaaaga tgaaaagggc aatttattgt cgcgtgaagc aatgattgaa     420

cgtatgcgcg aatacatcca cacagtagta ggccgctacc gtgggaaagt ttacgcttgg     480

gacgtcgtta atgaagccgt ggacgaaaac cagccagacg gactgcgtcg ttcgttatgg     540

tatcaggtta tcggcccaga ttatattgag cttgcattta agtttgcgca tgaggctgac     600

ccagatgcat tgttatttta caacgactat aatgaattct ttccgaaaaa acgtgacatc     660

atctttaagt tggttaagga gatgcgcgaa aagggcgtcc caattcatgg gatcggaatg     720

caacagcatc ttacacttgc ggacaacgtg gggtggatcg atatcgcaat ccaaaaattt     780

aagactatca gtgggatcca aatccatatt acggaacttg acgtatctgt ctacaagagt     840

cgttctccaa gcattattta tcagacacct ccacttgaag tgcttaaaga acaagcggaa     900

ttttatcgta aactttttga gatttatcgc aagcacaccg acgtaattac caatgttaca     960

ttttgggggt tgaaggacga ctacagctgg ttgcgtttct tttttggccg tcgcaacgac    1020

tggccgctgc tttttgacga gaattatcaa ccgaaacctg cgttttggag tgttatcgaa    1080

tcagtaagca aggtcgacgg cgcaacaact acccctgtcc cagtctcagg atctctagag    1140

gtaaaggtaa acgattgggg tagtggtgct gagtatgatg tgactcttaa tttggatgga    1200

cagtatgact ggactgtgaa ggttaaacta gccccaggag ccactgtagg aagcttctgg    1260

agcgctaaca aacaagaggg gaatggctat gtcatcttca ctccagtaag ctggaataaa    1320

gggccgacag caacatttgg attcatagta aacggaccac aaggagacaa agtagaagaa    1380

ataaccctag aaataaacgg acaagtaatt gaagcttaa                           1419


<210> 18
<211> 1677
<212> DNA
<213> Artificial Sequence


<220> 
<223> X11 optimized with CBM9

<400> 18
atgaaaaagc tgattttcgc catcacattg ttgcttatag tctttactgt gataatcaaa      60

ggagggcctc agccgatgtc gtcgcaaatc ccctcactga aggatgtctt tcttcaagat     120

ttcaaaatcg gcgtggcact gccggtacgc gtcttctcta attccatgga tgttgagtta     180

attaccaaac atttcaattc gatgacagcg gaaaatgaga tgaagcccga atcgatttta     240

cgtcgtgatg cctcggggaa gatttattat gacttcaccg ttgctgaccg ctacatcgaa     300

tttgcacaga agcatggcat ggtggtacgc ggacacacat tagtttggca ctcccaaaca     360

ccggaatggt ttttcaaaga tgaaaagggc aatttattgt cgcgtgaagc aatgattgaa     420

cgtatgcgcg aatacatcca cacagtagta ggccgctacc gtgggaaagt ttacgcttgg     480

gacgtcgtta atgaagccgt ggacgaaaac cagccagacg gactgcgtcg ttcgttatgg     540

tatcaggtta tcggcccaga ttatattgag cttgcattta agtttgcgca tgaggctgac     600

ccagatgcat tgttatttta caacgactat aatgaattct ttccgaaaaa acgtgacatc     660

atctttaagt tggttaagga gatgcgcgaa aagggcgtcc caattcatgg gatcggaatg     720

caacagcatc ttacacttgc ggacaacgtg gggtggatcg atatcgcaat ccaaaaattt     780

aagactatca gtgggatcca aatccatatt acggaacttg acgtatctgt ctacaagagt     840

cgttctccaa gcattattta tcagacacct ccacttgaag tgcttaaaga acaagcggaa     900

ttttatcgta aactttttga gatttatcgc aagcacaccg acgtaattac caatgttaca     960

ttttgggggt tgaaggacga ctacagctgg ttgcgtttct tttttggccg tcgcaacgac    1020

tggccgctgc tttttgacga gaattatcaa ccgaaacctg cgttttggag tgttatcgaa    1080

tcagtaagca aggtcgacgg cgcaggaata atggtagcga cagcaaaata cggaacaccg    1140

gtcatcgatg gagagataga cgagatctgg aacacgacag aggagataga gacgaaagcg    1200

gtggccatgg gatcgcttga caagaacgcg acagcgaaag tgagggtgct gtgggacgag    1260

aactacctgt acgtacttgc aatcgtgaaa gaccccgttc tgaacaaaga caacagcaac    1320

ccgtgggaac aggattccgt ggagatcttc atcgacgaga acaaccacaa gacaggatac    1380

tacgaagacg acgacgcaca gttcagggtg aactacatga acgagcagac gtttggaacg    1440

ggaggaagtc cagcgaggtt caagacagcg gtgaaactga tcgaaggagg atacatagtt    1500

gaggcagcga tcaagtggaa gacgatcaaa cccacaccga acacggtgat aggattcaac    1560

atccaggtga acgatgcgaa cgagaaaggg cagagggtcg gtatcatctc ctggagcgat    1620

cccacaaaca acagctggag agatccttca aagttcggta acctcaggct catcaag       1677


<210> 19
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 19
cccgaaaagt gccacctg                                                  18


<210> 20
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> primer

<400> 20
gttctgaggt cattactgg                                                 19


