﻿                         序列表

<110>  上海君实生物医药科技股份有限公司
       苏州君盟生物医药科技有限公司

<120>  靶向PD-1/PD-L1和TGF-β的双功能蛋白及其医药用途

<130>  MTP21044

<160>  33

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  5
<212>  PRT
<213>  PD-1抗体的HCDR1(Artificial Sequence)

<400>  1
Asp Tyr Glu Met His 
1               5   

<210>  2
<211>  17
<212>  PRT
<213>  PD-1抗体的HCDR2(Artificial Sequence)

<400>  2
Val Ile Glu Ser Glu Thr Gly Gly Thr Ala Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      
Gly 
    

<210>  3
<211>  16
<212>  PRT
<213>  PD-1抗体的HCDR3(Artificial Sequence)

<400>  3
Glu Gly Ile Thr Thr Val Ala Thr Thr Tyr Tyr Trp Tyr Phe Asp Val 
1               5                   10                  15      

<210>  4
<211>  16
<212>  PRT
<213>  PD-1抗体的LCDR1(Artificial Sequence)

<400>  4
Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu 
1               5                   10                  15      

<210>  5
<211>  7
<212>  PRT
<213>  PD-1抗体的LCDR2(Artificial Sequence)

<400>  5
Lys Val Ser Asn Arg Phe Ser 
1               5           

<210>  6
<211>  9
<212>  PRT
<213>  PD-1抗体的LCDR3(Artificial Sequence)

<400>  6
Phe Gln Gly Ser His Val Pro Leu Thr 
1               5                   

<210>  7
<211>  125
<212>  PRT
<213>  PD-1抗体重链可变区(VH)(Artificial Sequence)

<400>  7
Gln Gly Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          
Glu Met His Trp Val Arg Gln Ala Pro Ile His Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Val Ile Glu Ser Glu Thr Gly Gly Thr Ala Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Glu Gly Ile Thr Thr Val Ala Thr Thr Tyr Tyr Trp Tyr Phe 
            100                 105                 110         
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 

<210>  8
<211>  112
<212>  PRT
<213>  PD-1抗体重链可变区(VH)(Artificial Sequence)

<400>  8
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      
Ser His Val Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         

<210>  9
<211>  227
<212>  PRT
<213>  免疫球蛋白Fc结构域(Artificial Sequence)

<400>  9
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 
Leu Ser Leu 
225         

<210>  10
<211>  136
<212>  PRT
<213>  TGF-βRII胞外结构域(Artificial Sequence)

<400>  10
Ile Pro Pro His Val Gln Lys Ser Val Asn Asn Asp Met Ile Val Thr 
1               5                   10                  15      
Asp Asn Asn Gly Ala Val Lys Phe Pro Gln Leu Cys Lys Phe Cys Asp 
            20                  25                  30          
Val Arg Phe Ser Thr Cys Asp Asn Gln Lys Ser Cys Met Ser Asn Cys 
        35                  40                  45              
Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln Glu Val Cys Val Ala Val 
    50                  55                  60                  
Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu Glu Thr Val Cys His Asp 
65                  70                  75                  80  
Pro Lys Leu Pro Tyr His Asp Phe Ile Leu Glu Asp Ala Ala Ser Pro 
                85                  90                  95      
Lys Cys Ile Met Lys Glu Lys Lys Lys Pro Gly Glu Thr Phe Phe Met 
            100                 105                 110         
Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp Asn Ile Ile Phe Ser Glu 
        115                 120                 125             
Glu Tyr Asn Thr Ser Asn Pro Asp 
    130                 135     

<210>  11
<211>  450
<212>  PRT
<213>  PD-1抗体重链(Artificial Sequence)

<400>  11
Gln Gly Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          
Glu Met His Trp Val Arg Gln Ala Pro Ile His Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Val Ile Glu Ser Glu Thr Gly Gly Thr Ala Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Glu Gly Ile Thr Thr Val Ala Thr Thr Tyr Tyr Trp Tyr Phe 
            100                 105                 110         
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser 
    130                 135                 140                 
Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
145                 150                 155                 160 
Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170                 175     
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
            180                 185                 190         
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys 
        195                 200                 205             
Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu 
    210                 215                 220                 
Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu 
225                 230                 235                 240 
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                245                 250                 255     
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            260                 265                 270         
Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
        275                 280                 285             
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    290                 295                 300                 
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
305                 310                 315                 320 
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser 
                325                 330                 335     
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            340                 345                 350         
Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val 
        355                 360                 365             
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    370                 375                 380                 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
385                 390                 395                 400 
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr 
                405                 410                 415     
Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val 
            420                 425                 430         
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        435                 440                 445             
Ser Leu 
    450 

<210>  12
<211>  219
<212>  PRT
<213>  PD-1抗体轻链(Artificial Sequence)

<400>  12
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      
Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              
Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  
Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      
Ser His Val Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 

<210>  13
<211>  607
<212>  PRT
<213>  双功能蛋白JS-TZO3第一亚基(Artificial Sequence)

<400>  13
Gln Gly Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          
Glu Met His Trp Val Arg Gln Ala Pro Ile His Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Val Ile Glu Ser Glu Thr Gly Gly Thr Ala Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Glu Gly Ile Thr Thr Val Ala Thr Thr Tyr Tyr Trp Tyr Phe 
            100                 105                 110         
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser 
    130                 135                 140                 
Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
145                 150                 155                 160 
Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170                 175     
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
            180                 185                 190         
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys 
        195                 200                 205             
Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu 
    210                 215                 220                 
Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu 
225                 230                 235                 240 
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                245                 250                 255     
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            260                 265                 270         
Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
        275                 280                 285             
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    290                 295                 300                 
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
305                 310                 315                 320 
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser 
                325                 330                 335     
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            340                 345                 350         
Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val 
        355                 360                 365             
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    370                 375                 380                 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
385                 390                 395                 400 
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr 
                405                 410                 415     
Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val 
            420                 425                 430         
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        435                 440                 445             
Ser Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    450                 455                 460                 
Ser Gly Gly Gly Gly Ser Gly Ile Pro Pro His Val Gln Lys Ser Val 
465                 470                 475                 480 
Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
                485                 490                 495     
Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
            500                 505                 510         
Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
        515                 520                 525             
Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
    530                 535                 540                 
Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
545                 550                 555                 560 
Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
                565                 570                 575     
Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
            580                 585                 590         
Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp 
        595                 600                 605         

<210>  14
<211>  152
<212>  DNA
<213>  引物TGFβR2-G4SBSPQIFV2(Artificial Sequence)

<400>  14
atgcgctctt caggcggcgg cggcagcggc ggcggcggca gcggcggcgg cggcagcggc        60
ggcggcggca gcggcatccc cccccacgca gcggcggcgg cggcagcggc ggcggcggca       120
gcggcatccc cccccacgtg cagaagtccg tg                                     152

<210>  15
<211>  47
<212>  DNA
<213>  引物TGFβR2-BSPQIRV2(Artificial Sequence)

<400>  15
tagcgctctt ctcgctcagt cggggttgct ggtgttgtac tcctcgc                      47

<210>  16
<211>  1821
<212>  DNA
<213>  双功能蛋白JS-TZO3第一亚基的编码核酸(Artificial Sequence)

<400>  16
cagggccagc tggtgcagag cggcgccgag gtgaagaagc ccggcgccag cgtgaaggtg        60
agctgcaagg ccagcggcta caccttcacc gactacgaga tgcactgggt gagacaggcc       120
cccatccacg gcctggagtg gatcggcgtg atcgagagcg agaccggcgg caccgcctac       180
aaccagaagt tcaagggcag agtgaccatc accgccgaca agagcaccag caccgcctac       240
atggagctga gcagcctgag aagcgaggac accgccgtgt actactgcgc cagagagggc       300
atcaccaccg tggccaccac ctactactgg tacttcgacg tgtggggcca gggcaccacc       360
gtgaccgtga gcagcgctag caccaagggc cccagcgtgt tccccctggc cccttgcagc       420
agaagcacca gcgagagcac agccgccctg ggctgcctgg tgaaggacta cttccccgag       480
cccgtgaccg tgtcctggaa cagcggcgct ctgaccagcg gcgtgcatac cttccccgcc       540
gtgctccaga gcagcggact gtactccctg agcagcgtgg tgaccgtgcc ttccagcagc       600
ctgggcacca agacctacac ctgcaacgtg gaccacaagc ccagcaacac caaggtggac       660
aagagagtgg agagcaagta cggccctccc tgcccccctt gccctgcccc cgagttcctg       720
ggcggaccta gcgtgttcct gttccccccc aagcccaagg acaccctgat gatcagcaga       780
acccccgagg tgacctgcgt ggtggtggac gtgtcccagg aggaccccga ggtccagttt       840
aattggtacg tggacggcgt ggaagtgcat aacgccaaga ccaagcccag agaggagcag       900
ttcaacagca cctacagagt ggtgtccgtg ctgaccgtgc tgcaccagga ctggctgaac       960
ggcaaggaat acaagtgcaa ggtctccaac aagggcctgc ctagcagcat cgagaagacc      1020
atcagcaagg ccaagggcca gccacgggag ccccaggtct acaccctgcc acctagccaa      1080
gaggagatga ccaagaacca ggtgtccctg acctgtctgg tgaaaggctt ctatcccagc      1140
gatatcgccg tggagtggga gagcaacggc cagcccgaga acaactacaa gaccaccccc      1200
cctgtgctgg acagcgacgg cagcttcttc ctgtactcca gactgaccgt ggacaagtcc      1260
agatggcagg agggcaacgt cttcagctgc tccgtgatgc acgaggccct gcacaaccac      1320
tacacccaga agtccctgag cctgagcctg ggcggcggcg gcagcggcgg cggcggcagc      1380
ggcggcggcg gcagcggcgg cggcggcagc ggcatccccc cccacgtgca gaagtccgtg      1440
aacaacgaca tgatcgtgac cgacaacaac ggcgccgtga agttccccca gctgtgcaag      1500
ttctgcgacg tgagattcag cacctgcgac aaccagaagt cctgcatgag caactgcagc      1560
atcaccagca tctgcgagaa gccccaggag gtgtgcgtgg ccgtgtggag aaagaacgac      1620
gagaacatca ccctggagac cgtgtgccac gaccccaagc tgccctacca cgacttcatc      1680
ctggaggacg ccgccagccc caagtgcatc atgaaggaga agaagaagcc cggcgagacc      1740
ttcttcatgt gcagctgcag cagcgacgag tgcaacgaca acatcatctt cagcgaggag      1800
tacaacacca gcaaccccga c                                                1821

<210>  17
<211>  657
<212>  DNA
<213>  双功能蛋白JS-TZO3第二亚基的编码核酸(Artificial Sequence)

<400>  17
gatgtggtga tgacccagag cccgctgagc ctgccggtga ccctgggcca gccggcgagc        60
attagctgcc gcagcagcca gagcattgtg catagcaacg gcaacaccta tctggaatgg       120
tatctgcaga aaccgggcca gagcccgcag ctgctgattt ataaagtgag caaccgcttt       180
agcggcgtgc cggatcgctt tagcggcagc ggcagcggca ccgattttac cctgaaaatt       240
agccgcgtgg aagcggaaga tgtgggcgtg tattattgct ttcagggcag ccatgtgccg       300
ctgacctttg gccagggcac caaactggaa attaaacgaa ctgtggctgc accatctgtc       360
ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg       420
ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa       480
tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctc       540
agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa       600
gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg agagtgt          657

<210>  18
<211>  8
<212>  PRT
<213>  PD-L1抗体的HCDR1(Artificial Sequence)

<400>  18
Gly Asp Ser Ile Thr Arg Gly Tyr 
1               5               

<210>  19
<211>  7
<212>  PRT
<213>  PD-L1抗体的HCDR2(Artificial Sequence)

<400>  19
Ile Ser Tyr Thr Gly Ser Thr 
1               5           

<210>  20
<211>  12
<212>  PRT
<213>  PD-L1抗体的HCDR3(Artificial Sequence)

<400>  20
Ala Thr Ser Thr Gly Trp Leu Asp Pro Val Asp Tyr 
1               5                   10          

<210>  21
<211>  6
<212>  PRT
<213>  PD-L1抗体的LCDR1(Artificial Sequence)

<400>  21
Gln Asn Val Asp Thr Ser 
1               5       

<210>  22
<211>  3
<212>  PRT
<213>  PD-L1抗体的LCDR2(Artificial Sequence)

<400>  22
Ser Ala Ser 
1           

<210>  23
<211>  9
<212>  PRT
<213>  PD-L1抗体的LCDR3(Artificial Sequence)

<400>  23
Gln Gln Tyr Tyr Gly Tyr Pro Phe Thr 
1               5                   

<210>  24
<212>  PRT
<213>  PD-L1抗体的重链可变区(VH)(Artificial Sequence)

<400>  24
Gln Gly Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Ile Thr Arg Gly 
            20                  25                  30          
Tyr Trp Asn Trp Ile Arg Lys His Pro Gly Lys Gly Leu Glu Tyr Ile 
        35                  40                  45              
Gly Tyr Ile Ser Tyr Thr Gly Ser Thr Tyr Ser Asn Leu Ser Leu Lys 
    50                  55                  60                  
Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Tyr Tyr Leu 
65                  70                  75                  80  
Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Thr Ser Thr Gly Trp Leu Asp Pro Val Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Leu Val Thr Val Ser Ser 
        115             

<210>  25
<211>  107
<212>  PRT
<213>  PD-L1抗体的轻链可变区(VL)(Artificial Sequence)

<400>  25
Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      
Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Asn Val Asp Thr Ser 
            20                  25                  30          
Val Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Ala Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Phe Arg Tyr Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  
Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Tyr Tyr Gly Tyr Pro Phe 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         

<210>  26
<211>  445
<212>  PRT
<213>  PD-L1抗体重链(Artificial Sequence)

<400>  26
Gln Gly Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Ile Thr Arg Gly 
            20                  25                  30          
Tyr Trp Asn Trp Ile Arg Lys His Pro Gly Lys Gly Leu Glu Tyr Ile 
        35                  40                  45              
Gly Tyr Ile Ser Tyr Thr Gly Ser Thr Tyr Ser Asn Leu Ser Leu Lys 
    50                  55                  60                  
Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Tyr Tyr Leu 
65                  70                  75                  80  
Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Thr Ser Thr Gly Trp Leu Asp Pro Val Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             
Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         
Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             
Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 
Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     
Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 
Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         
Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 
Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     
Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        435                 440                 445 

<210>  27
<211>  214
<212>  PRT
<213>  PD-L1抗体轻链(Artificial Sequence)

<400>  27
Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      
Glu Arg Ala Thr Ile Asn Cys Lys Ala Ser Gln Asn Val Asp Thr Ser 
            20                  25                  30          
Val Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Ala Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Phe Arg Tyr Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  
Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Tyr Tyr Gly Tyr Pro Phe 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         
Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             
Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 
Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     
Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         
Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             
Phe Asn Arg Gly Glu Cys 
    210                 

<210>  28
<211>  600
<212>  PRT
<213>  双功能蛋白JS-TZO4第一亚基(Artificial Sequence)

<400>  28
Gln Gly Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Ile Thr Arg Gly 
            20                  25                  30          
Tyr Trp Asn Trp Ile Arg Lys His Pro Gly Lys Gly Leu Glu Tyr Ile 
        35                  40                  45              
Gly Tyr Ile Ser Tyr Thr Gly Ser Thr Tyr Ser Asn Leu Ser Leu Lys 
    50                  55                  60                  
Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Tyr Tyr Leu 
65                  70                  75                  80  
Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Thr Ser Thr Gly Trp Leu Asp Pro Val Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             
Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         
Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             
Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 
Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     
Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 
Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         
Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 
Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     
Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Gly Gly Ser 
        435                 440                 445             
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    450                 455                 460                 
Ile Pro Pro His Val Gln Lys Ser Val Asn Asn Asp Met Ile Val Thr 
465                 470                 475                 480 
Asp Asn Asn Gly Ala Val Lys Phe Pro Gln Leu Cys Lys Phe Cys Asp 
                485                 490                 495     
Val Arg Phe Ser Thr Cys Asp Asn Gln Lys Ser Cys Met Ser Asn Cys 
            500                 505                 510         
Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln Glu Val Cys Val Ala Val 
        515                 520                 525             
Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu Glu Thr Val Cys His Asp 
    530                 535                 540                 
Pro Lys Leu Pro Tyr His Asp Phe Ile Leu Glu Asp Ala Ala Ser Pro 
545                 550                 555                 560 
Lys Cys Ile Met Lys Glu Lys Lys Lys Pro Gly Glu Thr Phe Phe Met 
                565                 570                 575     
Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp Asn Ile Ile Phe Ser Glu 
            580                 585                 590         
Glu Tyr Asn Thr Ser Asn Pro Asp 
        595                 600 

<210>  29
<211>  1803
<212>  DNA
<213>  JS-TZO4第一亚基核苷酸(Artificial Sequence)

<400>  29
cagggccagc tgcaggagag cggccccagc ctggtgaagc ccagccagac cctgagcctg        60
acctgcaccg tgagcggcga cagcatcacc agaggctact ggaactggat cagaaagcac       120
cccggcaagg gcctggagta catcggctac atcagctaca ccggcagcac ctacagcaac       180
ctgagcctga agtccagagt gaccatcagc agagacacca gcaagaacca gtactacctg       240
aagctgagca gcgtgaccgc cgccgacacc gccgtgtact actgcgccac cagcaccggc       300
tggctggacc ccgtggacta ctggggccag ggcaccctgg tgaccgtgag cagcgctagc       360
accaagggcc ccagcgtgtt ccccctggcc ccttgcagca gaagcaccag cgagagcaca       420
gccgccctgg gctgcctggt gaaggactac ttccccgagc ccgtgaccgt gtcctggaac       480
agcggcgctc tgaccagcgg cgtgcatacc ttccccgccg tgctccagag cagcggactg       540
tactccctga gcagcgtggt gaccgtgcct tccagcagcc tgggcaccaa gacctacacc       600
tgcaacgtgg accacaagcc cagcaacacc aaggtggaca agagagtgga gagcaagtac       660
ggccctccct gccccccttg ccctgccccc gagttcctgg gcggacctag cgtgttcctg       720
ttccccccca agcccaagga caccctgatg atcagcagaa cccccgaggt gacctgcgtg       780
gtggtggacg tgtcccagga ggaccccgag gtccagttta attggtacgt ggacggcgtg       840
gaagtgcata acgccaagac caagcccaga gaggagcagt tcaacagcac ctacagagtg       900
gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaggaata caagtgcaag       960
gtctccaaca agggcctgcc tagcagcatc gagaagacca tcagcaaggc caagggccag      1020
ccacgggagc cccaggtcta caccctgcca cctagccaag aggagatgac caagaaccag      1080
gtgtccctga cctgtctggt gaaaggcttc tatcccagcg atatcgccgt ggagtgggag      1140
agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc      1200
agcttcttcc tgtactccag actgaccgtg gacaagtcca gatggcagga gggcaacgtc      1260
ttcagctgct ccgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc      1320
ctgagcctgg gcggcggcgg cagcggcggc ggcggcagcg gcggcggcgg cagcggcggc      1380
ggcggcagcg gcatcccccc ccacgtgcag aagtccgtga acaacgacat gatcgtgacc      1440
gacaacaacg gcgccgtgaa gttcccccag ctgtgcaagt tctgcgacgt gagattcagc      1500
acctgcgaca accagaagtc ctgcatgagc aactgcagca tcaccagcat ctgcgagaag      1560
ccccaggagg tgtgcgtggc cgtgtggaga aagaacgacg agaacatcac cctggagacc      1620
gtgtgccacg accccaagct gccctaccac gacttcatcc tggaggacgc cgccagcccc      1680
aagtgcatca tgaaggagaa gaagaagccc ggcgagacct tcttcatgtg cagctgcagc      1740
agcgacgagt gcaacgacaa catcatcttc agcgaggagt acaacaccag caaccccgac      1800
tga                                                                    1803

<210>  30
<211>  645
<212>  DNA
<213>  JS-TZO4第二亚基核苷酸(Artificial Sequence)

<400>  30
gacatcgtga tgacccagag ccccgacagc ctggccgtga gcctgggcga gagagccacc        60
atcaactgca aggccagcca gaacgtggac accagcgtgg cctggttcca gcagaagccc       120
ggccagcccc ccaaggccct gatctacagc gccagcttca gatacagcgg cgtgcccgac       180
agattcagcg gcagcggcag cggcaccgac ttcaccctga ccatcagcag cctgcaggcc       240
gaggacgtgg ccgtgtactt ctgccagcag tactacggct accccttcac cttcggccag       300
ggcaccaagc tggagatcaa gcgaactgtg gctgcaccat ctgtcttcat cttcccgcca       360
tctgatgagc agttgaaatc tggaactgcc tctgttgtgt gcctgctgaa taacttctat       420
cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg taactcccag       480
gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag caccctgacg       540
ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc       600
ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gttag                       645

<210>  31
<211>  443
<212>  PRT
<213>  用于双功能蛋白JS-TZO4构建的PD-L1抗体重链(Artificial Sequence)

<400>  31
Gln Gly Gln Leu Gln Glu Ser Gly Pro Ser Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Ile Thr Arg Gly 
            20                  25                  30          
Tyr Trp Asn Trp Ile Arg Lys His Pro Gly Lys Gly Leu Glu Tyr Ile 
        35                  40                  45              
Gly Tyr Ile Ser Tyr Thr Gly Ser Thr Tyr Ser Asn Leu Ser Leu Lys 
    50                  55                  60                  
Ser Arg Val Thr Ile Ser Arg Asp Thr Ser Lys Asn Gln Tyr Tyr Leu 
65                  70                  75                  80  
Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      
Thr Ser Thr Gly Trp Leu Asp Pro Val Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             
Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         
Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             
Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 
Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     
Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             
Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 
Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         
Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 
Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     
Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu 
        435                 440             

<210>  32
<211>  232
<212>  PRT
<213>  免疫球蛋白Fc结构域(Artificial Sequence)

<400>  32
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         

<210>  33
<211>  229
<212>  PRT
<213>  免疫球蛋白Fc结构域(Artificial Sequence)

<400>  33
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 
Leu Ser Leu Gly Lys 
225                 

