                         SEQUENCE LISTING

<110>  Danisco US Inc.
       Guo , Feng
       Hao, Helong
       Kelemen, Bradley   R
       Liu , Amy   Deming
       Ni, Kefeng
       Qian, Zhen
       Tang, Zhongmei
 
<120>  ESTERASE AND METHODS OF USE, THEREOF

<130>  NB41838-WO-PCT

<140>  PCT/US2021/50611
<141>  2021-09-16

<150>  PCT/CN2020/115574
<151>  2020-09-16

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  265
<212>  PRT
<213>  Dactylosporangium aurantiacum

<400>  1

Met Gly Gly Gly Val Ala Ala Val Ala Thr Pro Ala Leu Ala Asp Glu 
1               5                   10                  15      


Ile Gly Gln Ala Pro Thr Ala Ser Asn Ile Thr Gly Asn Gly Ser Phe 
            20                  25                  30          


Ala Thr Thr Ser Glu Ser Ile Ser Ser Leu Val Ser Gly Phe Gly Gly 
        35                  40                  45              


Gly Arg Val Tyr Tyr Pro Thr Ala Thr Gly Arg Tyr Pro Val Ile Ala 
    50                  55                  60                  


Ile Ser Pro Gly Phe Thr Ala Thr Trp Ser Ser Leu Ala Trp Ile Gly 
65                  70                  75                  80  


Pro Arg Leu Ser Ser Trp Gly Phe Val Val Val Gly Ile Glu Thr Asn 
                85                  90                  95      


Ser Ile Tyr Asp Gln Pro Gly Ser Arg Gly Asn Gln Leu Leu Ala Ala 
            100                 105                 110         


Leu Asn Trp Ala Val Asn Ser Ser Ser Thr Ala Val Arg Ser Arg Val 
        115                 120                 125             


Asp Gly Ser Arg Arg Gly Val Ala Gly His Ser Met Gly Gly Gly Gly 
    130                 135                 140                 


Thr Leu Glu Ala Leu Ala Ala Asp Thr Ser Gly Leu Val Lys Ala Gly 
145                 150                 155                 160 


Val Pro Leu Ala Pro Trp Asn Thr Asp Lys Ile Trp Ser Asn Val Ser 
                165                 170                 175     


Glu Pro Val Leu Ile Val Gly Gly Gln Ala Asp Thr Ile Ala Pro Val 
            180                 185                 190         


Ala Thr His Ser Val Pro Phe Tyr Asn Thr Leu Ala Gly Pro Lys Thr 
        195                 200                 205             


Tyr Val Glu Leu Thr Gly Ala Ser His Phe Phe Pro Gln Thr Thr Asn 
    210                 215                 220                 


Ala Thr Thr Ser Arg Ala Leu Val Ser Trp Phe Lys Arg Trp Leu Asn 
225                 230                 235                 240 


Gln Asp Ser Arg Phe Thr Pro Tyr Thr Cys Gly Phe Gly Gly Leu Ala 
                245                 250                 255     


Val Ser Asp Phe Arg Ser Asn Ala Cys 
            260                 265 


<210>  2
<211>  251
<212>  PRT
<213>  Dactylosporangium aurantiacum

<400>  2

Asp Glu Ile Gly Gln Ala Pro Thr Ala Ser Asn Ile Thr Gly Asn Gly 
1               5                   10                  15      


Ser Phe Ala Thr Thr Ser Glu Ser Ile Ser Ser Leu Val Ser Gly Phe 
            20                  25                  30          


Gly Gly Gly Arg Val Tyr Tyr Pro Thr Ala Thr Gly Arg Tyr Pro Val 
        35                  40                  45              


Ile Ala Ile Ser Pro Gly Phe Thr Ala Thr Trp Ser Ser Leu Ala Trp 
    50                  55                  60                  


Ile Gly Pro Arg Leu Ser Ser Trp Gly Phe Val Val Val Gly Ile Glu 
65                  70                  75                  80  


Thr Asn Ser Ile Tyr Asp Gln Pro Gly Ser Arg Gly Asn Gln Leu Leu 
                85                  90                  95      


Ala Ala Leu Asn Trp Ala Val Asn Ser Ser Ser Thr Ala Val Arg Ser 
            100                 105                 110         


Arg Val Asp Gly Ser Arg Arg Gly Val Ala Gly His Ser Met Gly Gly 
        115                 120                 125             


Gly Gly Thr Leu Glu Ala Leu Ala Ala Asp Thr Ser Gly Leu Val Lys 
    130                 135                 140                 


Ala Gly Val Pro Leu Ala Pro Trp Asn Thr Asp Lys Ile Trp Ser Asn 
145                 150                 155                 160 


Val Ser Glu Pro Val Leu Ile Val Gly Gly Gln Ala Asp Thr Ile Ala 
                165                 170                 175     


Pro Val Ala Thr His Ser Val Pro Phe Tyr Asn Thr Leu Ala Gly Pro 
            180                 185                 190         


Lys Thr Tyr Val Glu Leu Thr Gly Ala Ser His Phe Phe Pro Gln Thr 
        195                 200                 205             


Thr Asn Ala Thr Thr Ser Arg Ala Leu Val Ser Trp Phe Lys Arg Trp 
    210                 215                 220                 


Leu Asn Gln Asp Ser Arg Phe Thr Pro Tyr Thr Cys Gly Phe Gly Gly 
225                 230                 235                 240 


Leu Ala Val Ser Asp Phe Arg Ser Asn Ala Cys 
                245                 250     


<210>  3
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized sequence

<400>  3
gctggaaaag atgaaatcgg acaagcacct acagcatcaa atatcacggg aaacggttct       60

tttgcaacga cgtcagaatc tatttcttca ttagttagcg gctttggcgg cggacgcgtt      120

tattatccta cggcaacggg ccgctatccg gttatcgcaa tctctccggg gttcacagca      180

acgtggtctt ctctggcatg gatcggaccg agactgtctt catggggatt tgttgttgtt      240

ggcatcgaaa caaattctat atacgatcaa ccgggctcac gcggaaatca attactggca      300

gcacttaatt gggcagttaa tagctcatct acggcagttc gctcacgcgt tgatggatct      360

agaagaggcg ttgcaggcca ttcaatgggg ggcggcggaa cacttgaagc actggcagca      420

gatacgtcag gcctggttaa agcgggtgtt cctcttgcac cgtggaatac agataaaatc      480

tggtctaatg tttcagaacc tgttctgatt gttggaggac aagcagatac gatcgcaccg      540

gttgcaacac atagcgttcc gttttataat acacttgcag gacctaaaac ctatgttgaa      600

ctgacaggcg catcacattt ctttcctcaa acgacgaatg caacgacgag ccgcgcatta      660

gttagctggt ttaaaagatg gctgaatcaa gatagccgct ttacaccgta tacgtgcggc      720

tttggaggcc ttgcagttag cgattttcgc tctaatgcat gc                         762


<210>  4
<211>  254
<212>  PRT
<213>  Dactylosporangium aurantiacum

<400>  4

Ala Gly Lys Asp Glu Ile Gly Gln Ala Pro Thr Ala Ser Asn Ile Thr 
1               5                   10                  15      


Gly Asn Gly Ser Phe Ala Thr Thr Ser Glu Ser Ile Ser Ser Leu Val 
            20                  25                  30          


Ser Gly Phe Gly Gly Gly Arg Val Tyr Tyr Pro Thr Ala Thr Gly Arg 
        35                  40                  45              


Tyr Pro Val Ile Ala Ile Ser Pro Gly Phe Thr Ala Thr Trp Ser Ser 
    50                  55                  60                  


Leu Ala Trp Ile Gly Pro Arg Leu Ser Ser Trp Gly Phe Val Val Val 
65                  70                  75                  80  


Gly Ile Glu Thr Asn Ser Ile Tyr Asp Gln Pro Gly Ser Arg Gly Asn 
                85                  90                  95      


Gln Leu Leu Ala Ala Leu Asn Trp Ala Val Asn Ser Ser Ser Thr Ala 
            100                 105                 110         


Val Arg Ser Arg Val Asp Gly Ser Arg Arg Gly Val Ala Gly His Ser 
        115                 120                 125             


Met Gly Gly Gly Gly Thr Leu Glu Ala Leu Ala Ala Asp Thr Ser Gly 
    130                 135                 140                 


Leu Val Lys Ala Gly Val Pro Leu Ala Pro Trp Asn Thr Asp Lys Ile 
145                 150                 155                 160 


Trp Ser Asn Val Ser Glu Pro Val Leu Ile Val Gly Gly Gln Ala Asp 
                165                 170                 175     


Thr Ile Ala Pro Val Ala Thr His Ser Val Pro Phe Tyr Asn Thr Leu 
            180                 185                 190         


Ala Gly Pro Lys Thr Tyr Val Glu Leu Thr Gly Ala Ser His Phe Phe 
        195                 200                 205             


Pro Gln Thr Thr Asn Ala Thr Thr Ser Arg Ala Leu Val Ser Trp Phe 
    210                 215                 220                 


Lys Arg Trp Leu Asn Gln Asp Ser Arg Phe Thr Pro Tyr Thr Cys Gly 
225                 230                 235                 240 


Phe Gly Gly Leu Ala Val Ser Asp Phe Arg Ser Asn Ala Cys 
                245                 250                 


<210>  5
<211>  290
<212>  PRT
<213>  Ideonella sakaiensis

<400>  5

Met Asn Phe Pro Arg Ala Ser Arg Leu Met Gln Ala Ala Val Leu Gly 
1               5                   10                  15      


Gly Leu Met Ala Val Ser Ala Ala Ala Thr Ala Gln Thr Asn Pro Tyr 
            20                  25                  30          


Ala Arg Gly Pro Asn Pro Thr Ala Ala Ser Leu Glu Ala Ser Ala Gly 
        35                  40                  45              


Pro Phe Thr Val Arg Ser Phe Thr Val Ser Arg Pro Ser Gly Tyr Gly 
    50                  55                  60                  


Ala Gly Thr Val Tyr Tyr Pro Thr Asn Ala Gly Gly Thr Val Gly Ala 
65                  70                  75                  80  


Ile Ala Ile Val Pro Gly Tyr Thr Ala Arg Gln Ser Ser Ile Lys Trp 
                85                  90                  95      


Trp Gly Pro Arg Leu Ala Ser His Gly Phe Val Val Ile Thr Ile Asp 
            100                 105                 110         


Thr Asn Ser Thr Leu Asp Gln Pro Ser Ser Arg Ser Ser Gln Gln Met 
        115                 120                 125             


Ala Ala Leu Arg Gln Val Ala Ser Leu Asn Gly Thr Ser Ser Ser Pro 
    130                 135                 140                 


Ile Tyr Gly Lys Val Asp Thr Ala Arg Met Gly Val Met Gly Trp Ser 
145                 150                 155                 160 


Met Gly Gly Gly Gly Ser Leu Ile Ser Ala Ala Asn Asn Pro Ser Leu 
                165                 170                 175     


Lys Ala Ala Ala Pro Gln Ala Pro Trp Asp Ser Ser Thr Asn Phe Ser 
            180                 185                 190         


Ser Val Thr Val Pro Thr Leu Ile Phe Ala Cys Glu Asn Asp Ser Ile 
        195                 200                 205             


Ala Pro Val Asn Ser Ser Ala Leu Pro Ile Tyr Asp Ser Met Ser Arg 
    210                 215                 220                 


Asn Ala Lys Gln Phe Leu Glu Ile Asn Gly Gly Ser His Ser Cys Ala 
225                 230                 235                 240 


Asn Ser Gly Asn Ser Asn Gln Ala Leu Ile Gly Lys Lys Gly Val Ala 
                245                 250                 255     


Trp Met Lys Arg Phe Met Asp Asn Asp Thr Arg Tyr Ser Thr Phe Ala 
            260                 265                 270         


Cys Glu Asn Pro Asn Ser Thr Arg Val Ser Asp Phe Arg Thr Ala Asn 
        275                 280                 285             


Cys Ser 
    290 


