                         SEQUENCE LISTING

<110>  The Brigham and Women's Hospital, Inc.
       Agarwal, Shailesh
 
<120>  IN VIVO ADIPOSE BIOREACTOR AND KITS FOR THE PRODUCTION AND 
       DELIVERY OF BIOLOGIC AGENTS

<130>  129319.00795

<150>  US 63/078,655
<151>  2020-09-15

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  389
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - BMPR1A-Fc


<220>
<221>  MISC_FEATURE
<222>  (1)..(23)
<223>  signal peptide of BMPR1A

<220>
<221>  MISC_FEATURE
<222>  (24)..(152)
<223>  functional domain of BMPR1A

<220>
<221>  misc_feature
<222>  (153)..(158)
<223>  linker

<220>
<221>  misc_feature
<222>  (159)..(389)
<223>  Fc tag

<400>  1

Met Pro Gln Leu Tyr Ile Tyr Ile Arg Leu Leu Gly Ala Tyr Leu Phe 
1               5                   10                  15      


Ile Ile Ser Arg Val Gly Gly Gln Asn Leu Asp Ser Met Leu His Gly 
            20                  25                  30          


Thr Gly Met Lys Ser Asp Ser Asp Gln Lys Lys Ser Glu Asn Gly Val 
        35                  40                  45              


Thr Leu Ala Pro Glu Asp Thr Leu Pro Phe Leu Lys Cys Tyr Cys Ser 
    50                  55                  60                  


Gly His Cys Pro Asp Asp Ala Ile Asn Asn Thr Cys Ile Thr Asn Gly 
65                  70                  75                  80  


His Cys Phe Ala Ile Ile Glu Glu Asp Asp Gln Gly Glu Thr Thr Leu 
                85                  90                  95      


Ala Ser Gly Cys Met Lys Tyr Glu Gly Ser Asp Phe Gln Cys Lys Asp 
            100                 105                 110         


Ser Pro Lys Ala Gln Leu Arg Arg Thr Ile Glu Cys Cys Arg Thr Asn 
        115                 120                 125             


Leu Cys Asn Gln Tyr Leu Gln Pro Thr Leu Pro Pro Val Val Ile Gly 
    130                 135                 140                 


Pro Phe Phe Asp Gly Ser Ile Arg Ile Glu Gly Arg Met Asp Pro Lys 
145                 150                 155                 160 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                165                 170                 175     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            180                 185                 190         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        195                 200                 205             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    210                 215                 220                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
225                 230                 235                 240 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                245                 250                 255     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            260                 265                 270         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        275                 280                 285             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
    290                 295                 300                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
305                 310                 315                 320 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                325                 330                 335     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            340                 345                 350         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        355                 360                 365             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    370                 375                 380                 


Leu Ser Pro Gly Lys 
385                 


<210>  2
<211>  1167
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - BMPR1A-Fc


<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  signal peptide of BMPR1A

<220>
<221>  misc_feature
<222>  (70)..(456)
<223>  functional domain of BMPR1A

<220>
<221>  misc_feature
<222>  (457)..(474)
<223>  linker

<220>
<221>  misc_feature
<222>  (475)..(1167)
<223>  Fc tag

<400>  2
atgccgcagc tgtatattta tattcgcctg ctgggcgcgt atctgtttat tattagccgc       60

gtgggcggcc agaacctgga tagcatgctg catggcaccg gcatgaaaag cgatagcgat      120

cagaaaaaaa gcgaaaacgg cgtgaccctg gcgccggaag ataccctgcc gtttctgaaa      180

tgctattgca gcggccattg cccggatgat gcgattaaca acacctgcat taccaacggc      240

cattgctttg cgattattga agaagatgat cagggcgaaa ccaccctggc gagcggctgc      300

atgaaatatg aaggcagcga ttttcagtgc aaagatagcc cgaaagcgca gctgcgccgc      360

accattgaat gctgccgcac caacctgtgc aaccagtatc tgcagccgac cctgccgccg      420

gtggtgattg gcccgttttt tgatggcagc attcgcattg aaggccgcat ggatccgaaa      480

agctgcgata aaacccatac ctgcccgccg tgcccggcgc cggaactgct gggcggcccg      540

agcgtgtttc tgtttccgcc gaaaccgaaa gataccctga tgattagccg caccccggaa      600

gtgacctgcg tggtggtgga tgtgagccat gaagatccgg aagtgaaatt taactggtat      660

gtggatggcg tggaagtgca taacgcgaaa accaaaccgc gcgaagaaca gtataacagc      720

acctatcgcg tggtgagcgt gctgaccgtg ctgcatcagg attggctgaa cggcaaagaa      780

tataaatgca aagtgagcaa caaagcgctg ccggcgccga ttgaaaaaac cattagcaaa      840

gcgaaaggcc agccgcgcga accgcaggtg tataccctgc cgccgagccg cgatgaactg      900

accaaaaacc aggtgagcct gacctgcctg gtgaaaggct tttatccgag cgatattgcg      960

gtggaatggg aaagcaacgg ccagccggaa aacaactata aaaccacccc gccggtgctg     1020

gatagcgatg gcagcttttt tctgtatagc aaactgaccg tggataaaag ccgctggcag     1080

cagggcaacg tgtttagctg cagcgtgatg catgaagcgc tgcataacca ttatacccag     1140

aaaagcctga gcctgagccc gggcaaa                                         1167


<210>  3
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - TGFBII-Fc


<220>
<221>  MISC_FEATURE
<222>  (1)..(22)
<223>  signal peptide of TGFBII

<220>
<221>  MISC_FEATURE
<222>  (23)..(159)
<223>  functional domain of TGFBII

<220>
<221>  MISC_FEATURE
<222>  (160)..(161)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (162)..(392)
<223>  Fc tag

<400>  3

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Met 
145                 150                 155                 160 


Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                165                 170                 175     


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            180                 185                 190         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        195                 200                 205             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    210                 215                 220                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
225                 230                 235                 240 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                245                 250                 255     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            260                 265                 270         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        275                 280                 285             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    290                 295                 300                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
305                 310                 315                 320 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                325                 330                 335     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            340                 345                 350         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        355                 360                 365             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    370                 375                 380                 


Ser Leu Ser Leu Ser Pro Gly Lys 
385                 390         


<210>  4
<211>  1176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic - TGFBII-Fc


<220>
<221>  misc_feature
<222>  (1)..(66)
<223>  signal peptide of TGFBII

<220>
<221>  misc_feature
<222>  (67)..(477)
<223>  functional domain of TGFBII

<220>
<221>  misc_feature
<222>  (478)..(483)
<223>  linker

<220>
<221>  misc_feature
<222>  (484)..(1176)
<223>  Fc tag

<400>  4
atgggccgcg gcctgctgcg cggcctgtgg ccgctgcata ttgtgctgtg gacccgcatt       60

gcgagcacca ttccgccgca tgtgcagaaa agcgtgaaca acgatatgat tgtgaccgat      120

aacaacggcg cggtgaaatt tccgcagctg tgcaaatttt gcgatgtgcg ctttagcacc      180

tgcgataacc agaaaagctg catgagcaac tgcagcatta ccagcatttg cgaaaaaccg      240

caggaagtgt gcgtggcggt gtggcgcaaa aacgatgaaa acattaccct ggaaaccgtg      300

tgccatgatc cgaaactgcc gtatcatgat tttattctgg aagatgcggc gagcccgaaa      360

tgcattatga aagaaaaaaa aaaaccgggc gaaacctttt ttatgtgcag ctgcagcagc      420

gatgaatgca acgataacat tatttttagc gaagaatata acaccagcaa cccggatatg      480

gatccgaaaa gctgcgataa aacccatacc tgcccgccgt gcccggcgcc ggaactgctg      540

ggcggcccga gcgtgtttct gtttccgccg aaaccgaaag ataccctgat gattagccgc      600

accccggaag tgacctgcgt ggtggtggat gtgagccatg aagatccgga agtgaaattt      660

aactggtatg tggatggcgt ggaagtgcat aacgcgaaaa ccaaaccgcg cgaagaacag      720

tataacagca cctatcgcgt ggtgagcgtg ctgaccgtgc tgcatcagga ttggctgaac      780

ggcaaagaat ataaatgcaa agtgagcaac aaagcgctgc cggcgccgat tgaaaaaacc      840

attagcaaag cgaaaggcca gccgcgcgaa ccgcaggtgt ataccctgcc gccgagccgc      900

gatgaactga ccaaaaacca ggtgagcctg acctgcctgg tgaaaggctt ttatccgagc      960

gatattgcgg tggaatggga aagcaacggc cagccggaaa acaactataa aaccaccccg     1020

ccggtgctgg atagcgatgg cagctttttt ctgtatagca aactgaccgt ggataaaagc     1080

cgctggcagc agggcaacgt gtttagctgc agcgtgatgc atgaagcgct gcataaccat     1140

tatacccaga aaagcctgag cctgagcccg ggcaaa                               1176


<210>  5
<211>  532
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(532)
<223>  bone morphogenetic protein receptor type-1A (BMPR1A) precursor

<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  signal peptide

<220>
<221>  misc_feature
<222>  (24)..(152)
<223>  functional domain

<400>  5

Met Pro Gln Leu Tyr Ile Tyr Ile Arg Leu Leu Gly Ala Tyr Leu Phe 
1               5                   10                  15      


Ile Ile Ser Arg Val Gln Gly Gln Asn Leu Asp Ser Met Leu His Gly 
            20                  25                  30          


Thr Gly Met Lys Ser Asp Ser Asp Gln Lys Lys Ser Glu Asn Gly Val 
        35                  40                  45              


Thr Leu Ala Pro Glu Asp Thr Leu Pro Phe Leu Lys Cys Tyr Cys Ser 
    50                  55                  60                  


Gly His Cys Pro Asp Asp Ala Ile Asn Asn Thr Cys Ile Thr Asn Gly 
65                  70                  75                  80  


His Cys Phe Ala Ile Ile Glu Glu Asp Asp Gln Gly Glu Thr Thr Leu 
                85                  90                  95      


Ala Ser Gly Cys Met Lys Tyr Glu Gly Ser Asp Phe Gln Cys Lys Asp 
            100                 105                 110         


Ser Pro Lys Ala Gln Leu Arg Arg Thr Ile Glu Cys Cys Arg Thr Asn 
        115                 120                 125             


Leu Cys Asn Gln Tyr Leu Gln Pro Thr Leu Pro Pro Val Val Ile Gly 
    130                 135                 140                 


Pro Phe Phe Asp Gly Ser Ile Arg Trp Leu Val Leu Leu Ile Ser Met 
145                 150                 155                 160 


Ala Val Cys Ile Ile Ala Met Ile Ile Phe Ser Ser Cys Phe Cys Tyr 
                165                 170                 175     


Lys His Tyr Cys Lys Ser Ile Ser Ser Arg Arg Arg Tyr Asn Arg Asp 
            180                 185                 190         


Leu Glu Gln Asp Glu Ala Phe Ile Pro Val Gly Glu Ser Leu Lys Asp 
        195                 200                 205             


Leu Ile Asp Gln Ser Gln Ser Ser Gly Ser Gly Ser Gly Leu Pro Leu 
    210                 215                 220                 


Leu Val Gln Arg Thr Ile Ala Lys Gln Ile Gln Met Val Arg Gln Val 
225                 230                 235                 240 


Gly Lys Gly Arg Tyr Gly Glu Val Trp Met Gly Lys Trp Arg Gly Glu 
                245                 250                 255     


Lys Val Ala Val Lys Val Phe Phe Thr Thr Glu Glu Ala Ser Trp Phe 
            260                 265                 270         


Arg Glu Thr Glu Ile Tyr Gln Thr Val Leu Met Arg His Glu Asn Ile 
        275                 280                 285             


Leu Gly Phe Ile Ala Ala Asp Ile Lys Gly Thr Gly Ser Trp Thr Gln 
    290                 295                 300                 


Leu Tyr Leu Ile Thr Asp Tyr His Glu Asn Gly Ser Leu Tyr Asp Phe 
305                 310                 315                 320 


Leu Lys Cys Ala Thr Leu Asp Thr Arg Ala Leu Leu Lys Leu Ala Tyr 
                325                 330                 335     


Ser Ala Ala Cys Gly Leu Cys His Leu His Thr Glu Ile Tyr Gly Thr 
            340                 345                 350         


Gln Gly Lys Pro Ala Ile Ala His Arg Asp Leu Lys Ser Lys Asn Ile 
        355                 360                 365             


Leu Ile Lys Lys Asn Gly Ser Cys Cys Ile Ala Asp Leu Gly Leu Ala 
    370                 375                 380                 


Val Lys Phe Asn Ser Asp Thr Asn Glu Val Asp Val Pro Leu Asn Thr 
385                 390                 395                 400 


Arg Val Gly Thr Lys Arg Tyr Met Ala Pro Glu Val Leu Asp Glu Ser 
                405                 410                 415     


Leu Asn Lys Asn His Phe Gln Pro Tyr Ile Met Ala Asp Ile Tyr Ser 
            420                 425                 430         


Phe Gly Leu Ile Ile Trp Glu Met Ala Arg Arg Cys Ile Thr Gly Gly 
        435                 440                 445             


Ile Val Glu Glu Tyr Gln Leu Pro Tyr Tyr Asn Met Val Pro Ser Asp 
    450                 455                 460                 


Pro Ser Tyr Glu Asp Met Arg Glu Val Val Cys Val Lys Arg Leu Arg 
465                 470                 475                 480 


Pro Ile Val Ser Asn Arg Trp Asn Ser Asp Glu Cys Leu Arg Ala Val 
                485                 490                 495     


Leu Lys Leu Met Ser Glu Cys Trp Ala His Asn Pro Ala Ser Arg Leu 
            500                 505                 510         


Thr Ala Leu Arg Ile Lys Lys Thr Leu Ala Lys Met Val Glu Ser Gln 
        515                 520                 525             


Asp Val Lys Ile 
    530         


<210>  6
<211>  567
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(567)
<223>  TGF-beta receptor type-2 isoform B (TGFBII) precursor

<220>
<221>  MISC_FEATURE
<222>  (1)..(22)
<223>  signal peptide

<220>
<221>  MISC_FEATURE
<222>  (23)..(159)
<223>  functional domain

<400>  6

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Val Asn 
            180                 185                 190         


Arg Gln Gln Lys Leu Ser Ser Thr Trp Glu Thr Gly Lys Thr Arg Lys 
        195                 200                 205             


Leu Met Glu Phe Ser Glu His Cys Ala Ile Ile Leu Glu Asp Asp Arg 
    210                 215                 220                 


Ser Asp Ile Ser Ser Thr Cys Ala Asn Asn Ile Asn His Asn Thr Glu 
225                 230                 235                 240 


Leu Leu Pro Ile Glu Leu Asp Thr Leu Val Gly Lys Gly Arg Phe Ala 
                245                 250                 255     


Glu Val Tyr Lys Ala Lys Leu Lys Gln Asn Thr Ser Glu Gln Phe Glu 
            260                 265                 270         


Thr Val Ala Val Lys Ile Phe Pro Tyr Glu Glu Tyr Ala Ser Trp Lys 
        275                 280                 285             


Thr Glu Lys Asp Ile Phe Ser Asp Ile Asn Leu Lys His Glu Asn Ile 
    290                 295                 300                 


Leu Gln Phe Leu Thr Ala Glu Glu Arg Lys Thr Glu Leu Gly Lys Gln 
305                 310                 315                 320 


Tyr Trp Leu Ile Thr Ala Phe His Ala Lys Gly Asn Leu Gln Glu Tyr 
                325                 330                 335     


Leu Thr Arg His Val Ile Ser Trp Glu Asp Leu Arg Lys Leu Gly Ser 
            340                 345                 350         


Ser Leu Ala Arg Gly Ile Ala His Leu His Ser Asp His Thr Pro Cys 
        355                 360                 365             


Gly Arg Pro Lys Met Pro Ile Val His Arg Asp Leu Lys Ser Ser Asn 
    370                 375                 380                 


Ile Leu Val Lys Asn Asp Leu Thr Cys Cys Leu Cys Asp Phe Gly Leu 
385                 390                 395                 400 


Ser Leu Arg Leu Asp Pro Thr Leu Ser Val Asp Asp Leu Ala Asn Ser 
                405                 410                 415     


Gly Gln Val Gly Thr Ala Arg Tyr Met Ala Pro Glu Val Leu Glu Ser 
            420                 425                 430         


Arg Met Asn Leu Glu Asn Val Glu Ser Phe Lys Gln Thr Asp Val Tyr 
        435                 440                 445             


Ser Met Ala Leu Val Leu Trp Glu Met Thr Ser Arg Cys Asn Ala Val 
    450                 455                 460                 


Gly Glu Val Lys Asp Tyr Glu Pro Pro Phe Gly Ser Lys Val Arg Glu 
465                 470                 475                 480 


His Pro Cys Val Glu Ser Met Lys Asp Asn Val Leu Arg Asp Arg Gly 
                485                 490                 495     


Arg Pro Glu Ile Pro Ser Phe Trp Leu Asn His Gln Gly Ile Gln Met 
            500                 505                 510         


Val Cys Glu Thr Leu Thr Glu Cys Trp Asp His Asp Pro Glu Ala Arg 
        515                 520                 525             


Leu Thr Ala Gln Cys Val Ala Glu Arg Phe Ser Glu Leu Glu His Leu 
    530                 535                 540                 


Asp Arg Leu Ser Gly Arg Ser Cys Ser Glu Glu Lys Ile Pro Glu Asp 
545                 550                 555                 560 


Gly Ser Leu Asn Thr Thr Lys 
                565         


<210>  7
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - linker peptide used in BMPR1A-Fc

<400>  7

Ile Glu Gly Arg Met Asp 
1               5       


<210>  8
<211>  330
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(330)
<223>  fragment crystallizable region (Fc region) of immunoglobulin 
       heavy constant gamma 1

<400>  8

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  9
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic - Fc tag (amino acids 100-330 of SEQ ID NO:8)

<400>  9

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


