               SEQUENCE LISTING

<110> THERAVECTYS
      INSTITUT PASTEUR

<120> HIGH THROUGHPUT METHODS AND PRODUCTS
FOR SARS-COV-2 SERO-NEUTRALIZATION ASSAY

<130> PR90859

<150> US63/107,896
<151> 2020-10-30
<150> US63/080,939
<151> 2020-09-21

<160> 14

<170> BiSSAP 1.3.2

<210> 1
<211> 1273
<212> PRT
<213> Artificial Sequence

<223> "SARS-CoV-2 S Protein"

<220> 
<223> SARS-CoV-2 S Protein

<400> 1
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
        995                 1000                1005            
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
    1010                1015                1020                
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
1025                1030                1035                1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
                1045                1050                1055    
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
            1060                1065                1070        
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
        1075                1080                1085            
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
    1090                1095                1100                
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
1105                1110                1115                1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
                1125                1130                1135    
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
            1140                1145                1150        
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
        1155                1160                1165            
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
    1170                1175                1180                
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
1185                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 
                1205                1210                1215    
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 
            1220                1225                1230        
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 
        1235                1240                1245            
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 
    1250                1255                1260                
Leu Lys Gly Val Lys Leu His Tyr Thr 
1265                1270            

<210> 2
<211> 3819
<212> DNA
<213> Artificial Sequence

<223> "nucleotide sequence encoding SARS-CoV-2 S Protein"

<220> 
<223> nucleotide sequence encoding SARS-CoV-2 S Protein

<400> 2
atgttcgtgt ttctggtgct gctgccactg gtgtccagtc agtgcgtgaa cctgaccaca       60

cgaacacagc tgccaccagc ctacaccaat agcttcaccc gcggagtgta ctaccccgac      120

aaggtgttcc gcagcagcgt gctgcatagc acccaggatc tgtttctgcc cttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gcgcttcgat      240

aatcccgtgc tgcccttcaa cgatggcgtg tactttgcca gcaccgagaa gtccaatatc      300

atccgcggct ggatcttcgg caccacactg gatagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgcgtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc cattcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaaaatct acagcaagca caccccaatc aacctcgtgc gcgatctgcc acagggattc      660

agtgctctgg aacccctggt ggatctgccc atcggcatca acattacccg ctttcagaca      720

ctgctggccc tgcaccgcag ttacttgaca ccaggcgata gcagcagtgg atggacagct      780

ggtgccgccg cttactacgt tggatatctg cagccacgca cctttctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctcg atcccctgag cgagacaaag      900

tgcaccctga agtccttcac cgtcgagaag ggcatctacc agaccagcaa tttccgcgtg      960

cagcccaccg agagcatcgt gcgcttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccacacgctt tgcctccgtg tacgcctgga atcgcaagcg cattagcaac     1080

tgcgtggccg actactccgt gctgtacaat agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtcac ccaccaagct gaacgacctg tgcttcacca atgtgtacgc cgacagcttc     1200

gtgatccgcg gagatgaagt gcgacagatt gccccaggcc agaccggcaa gatcgccgac     1260

tacaattaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa cagcaacaac     1320

ctggattcca aagtcggcgg caactacaac tacctgtacc gcctgttccg caagagcaat     1380

ctgaagccct tcgagcgcga catcagcacc gaaatctacc aggccggaag caccccatgc     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggatt tcagcccaca     1500

aatggcgtgg gctaccagcc atatcgagtg gtggtgctga gcttcgaact gctgcatgct     1560

ccagctaccg tgtgcggccc caagaagagt accaacctgg tcaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagtaacaa gaagttcctg     1680

ccattccagc agtttggccg cgacattgcc gatacaaccg atgccgttcg cgatccccag     1740

accttggaga tcctggatat taccccatgc tccttcggcg gcgtgtccgt gattacacca     1800

ggcaccaata ccagcaacca ggtggccgtt ctgtaccagg atgtgaattg cacagaggtg     1860

cccgtggcca ttcacgccga tcaattgaca ccaacatggc gcgtgtactc caccggcagc     1920

aatgtgtttc aaacccgcgc tggatgcctg attggagccg agcacgtgaa caatagctac     1980

gagtgcgata tccccatcgg agccggaatc tgcgcctcct atcagaccca gaccaatagt     2040

ccacgacgag cccgaagtgt ggccagccag agcatcattg cctataccat gagcctgggc     2100

gccgagaata gcgtggccta ctccaacaac agcattgcta tccccaccaa cttcaccatc     2160

agcgtgacca ccgagatcct gccagtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gagatagcac cgagtgcagc aacctgctgc tgcagtacgg aagtttctgc     2280

acccagctga atcgcgccct gacaggcatt gccgtggaac aggataagaa cacccaagag     2340

gtgttcgccc aagtgaagca aatctacaag accccaccaa tcaaggattt cggcggcttc     2400

aatttcagcc agattctgcc cgatccaagc aagcccagca agcgcagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggattca tcaagcagta tggcgattgc     2520

ctgggcgata ttgccgcacg cgatctgatt tgcgcccaga agtttaacgg actgaccgtc     2580

ctgccaccac tgctgacaga tgagatgatc gcccagtaca caagtgccct gctggccgga     2640

accattacca gcggatggac atttggagcc ggtgccgctc tgcagattcc cttcgctatg     2700

cagatggcct accgcttcaa tggcattggc gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga ttcaggacag cctgagtagt     2820

accgccagcg ctctgggaaa gctgcaggat gtggtcaacc agaacgctca ggccctgaac     2880

accctggtta agcagctgag cagcaacttc ggcgccatca gtagcgtgct gaacgatatc     2940

ctgagccgcc tggataaggt ggaagccgag gtgcagatcg atcgcctgat taccggacgc     3000

ctgcagtccc tgcagaccta tgtgacacag cagctgatcc gagccgccga gattcgagct     3060

agtgctaatc tggccgccac caagatgagc gaatgtgtgc tgggacagag caagcgcgtg     3120

gacttttgcg gcaagggata ccacctgatg agcttcccac agagtgctcc acacggcgtg     3180

gtgtttctgc atgtgaccta cgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggaaaggc ccattttcca cgcgagggcg tgttcgttag caacggcact     3300

cattggttcg tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtcagcg gcaactgcga cgtcgtgatc ggcattgtga acaacaccgt gtacgatcca     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg agacattagc ggaatcaacg ccagcgtggt caacatccag     3540

aaagagattg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg gcaaatacga gcagtacatt aagtggccct ggtacatctg gctgggcttc     3660

attgccggac tgattgccat cgtgatggtc accattatgc tgtgctgcat gaccagttgc     3720

tgcagctgcc tgaagggatg ctgcagttgc ggaagctgct gcaagttcga cgaggatgat     3780

agcgagccag tgctgaaggg cgtcaagctg cactacacc                            3819


<210> 3
<211> 805
<212> PRT
<213> Artificial Sequence

<223> "human ACE2 Protein"

<220> 
<223> human ACE2 Protein

<400> 3
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             
Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 
Lys Ser Ala Leu Gly Asp Lys Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     
Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val 
            740                 745                 750         
Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg 
        755                 760                 765             
Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile 
    770                 775                 780                 
Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp 
785                 790                 795                 800 
Val Gln Thr Ser Phe 
                805 

<210> 4
<211> 2415
<212> DNA
<213> Artificial Sequence

<223> "nucleotide sequence encoding human ACE2 Protein"

<220> 
<223> nucleotide sequence encoding human ACE2 Protein

<400> 4
atgtcaagct cttcctggct ccttctcagc cttgttgctg taactgctgc tcagtccacc      60

attgaggaac aggccaagac atttttggac aagtttaacc acgaagccga agacctgttc     120

tatcaaagtt cacttgcttc ttggaattat aacaccaata ttactgaaga gaatgtccaa     180

aacatgaata atgctgggga caaatggtct gcctttttaa aggaacagtc cacacttgcc     240

caaatgtatc cactacaaga aattcagaat ctcacagtca agcttcagct gcaggctctt     300

cagcaaaatg ggtcttcagt gctctcagaa gacaagagca aacggttgaa cacaattcta     360

aatacaatga gcaccatcta cagtactgga aaagtttgta acccagataa tccacaagaa     420

tgcttattac ttgaaccagg tttgaatgaa ataatggcaa acagtttaga ctacaatgag     480

aggctctggg cttgggaaag ctggagatct gaggtcggca agcagctgag gccattatat     540

gaagagtatg tggtcttgaa aaatgagatg gcaagagcaa atcattatga ggactatggg     600

gattattgga gaggagacta tgaagtaaat ggggtagatg gctatgacta cagccgcggc     660

cagttgattg aagatgtgga acataccttt gaagagatta aaccattata tgaacatctt     720

catgcctatg tgagggcaaa gttgatgaat gcctatcctt cctatatcag tccaattgga     780

tgcctccctg ctcatttgct tggtgatatg tggggtagat tttggacaaa tctgtactct     840

ttgacagttc cctttggaca gaaaccaaac atagatgtta ctgatgcaat ggtggaccag     900

gcctgggatg cacagagaat attcaaggag gccgagaagt tctttgtatc tgttggtctt     960

cctaatatga ctcaaggatt ctgggaaaat tccatgctaa cggacccagg aaatgttcag    1020

aaagcagtct gccatcccac agcttgggac ctggggaagg gcgacttcag gatccttatg    1080

tgcacaaagg tgacaatgga cgacttcctg acagctcatc atgagatggg gcatatccag    1140

tatgatatgg catatgctgc acaacctttt ctgctaagaa atggagctaa tgaaggattc    1200

catgaagctg ttggggaaat catgtcactt tctgcagcca cacctaagca tttaaaatcc    1260

attggtcttc tgtcacccga ttttcaagaa gacaatgaaa cagaaataaa cttcctgctc    1320

aaacaagcac tcacgattgt tgggactctg ccatttactt acatgttaga gaagtggagg    1380

tggatggtct ttaaagggga aattcccaaa gaccagtgga tgaaaaagtg gtgggagatg    1440

aagcgagaga tagttggggt ggtggaacct gtgccccatg atgaaacata ctgtgacccc    1500

gcatctctgt tccatgtttc taatgattac tcattcattc gatattacac aaggaccctt    1560

taccaattcc agtttcaaga agcactttgt caagcagcta aacatgaagg ccctctgcac    1620

aaatgtgaca tctcaaactc tacagaagct ggacagaaac tgttcaatat gctgaggctt    1680

ggaaaatcag aaccctggac cctagcattg gaaaatgttg taggagcaaa gaacatgaat    1740

gtaaggccac tgctcaacta ctttgagccc ttatttacct ggctgaaaga ccagaacaag    1800

aattcttttg tgggatggag taccgactgg agtccatatg cagaccaaag catcaaagtg    1860

aggataagcc taaaatcagc tcttggagat aaagcatatg aatggaacga caatgaaatg    1920

tacctgttcc gatcatctgt tgcatatgct atgaggcagt actttttaaa agtaaaaaat    1980

cagatgattc tttttgggga ggaggatgtg cgagtggcta atttgaaacc aagaatctcc    2040

tttaatttct ttgtcactgc acctaaaaat gtgtctgata tcattcctag aactgaagtt    2100

gaaaaggcca tcaggatgtc ccggagccgt atcaatgatg ctttccgtct gaatgacaac    2160

agcctagagt ttctggggat acagccaaca cttggacctc ctaaccagcc ccctgtttcc    2220

atatggctga ttgtttttgg agttgtgatg ggagtgatag tggttggcat tgtcatcctg    2280

atcttcactg ggatcagaga tcggaagaag aaaaataaag caagaagtgg agaaaatcct    2340

tatgcctcca tcgatattag caaaggagaa aataatccag gattccaaaa cactgatgat    2400

gttcagacct ccttt                                                     2415


<210> 5
<211> 2436
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of BamHI-XhoI DNA insert (hACE2)"

<220> 
<223> Nucleotide sequence of BamHI-XhoI DNA insert (hACE2)

<400> 5
ggatccgcca ccatgtcaag ctcttcctgg ctccttctca gccttgttgc tgtaactgct      60

gctcagtcca ccattgagga acaggccaag acatttttgg acaagtttaa ccacgaagcc     120

gaagacctgt tctatcaaag ttcacttgct tcttggaatt ataacaccaa tattactgaa     180

gagaatgtcc aaaacatgaa taatgctggg gacaaatggt ctgccttttt aaaggaacag     240

tccacacttg cccaaatgta tccactacaa gaaattcaga atctcacagt caagcttcag     300

ctgcaggctc ttcagcaaaa tgggtcttca gtgctctcag aagacaagag caaacggttg     360

aacacaattc taaatacaat gagcaccatc tacagtactg gaaaagtttg taacccagat     420

aatccacaag aatgcttatt acttgaacca ggtttgaatg aaataatggc aaacagttta     480

gactacaatg agaggctctg ggcttgggaa agctggagat ctgaggtcgg caagcagctg     540

aggccattat atgaagagta tgtggtcttg aaaaatgaga tggcaagagc aaatcattat     600

gaggactatg gggattattg gagaggagac tatgaagtaa atggggtaga tggctatgac     660

tacagccgcg gccagttgat tgaagatgtg gaacatacct ttgaagagat taaaccatta     720

tatgaacatc ttcatgccta tgtgagggca aagttgatga atgcctatcc ttcctatatc     780

agtccaattg gatgcctccc tgctcatttg cttggtgata tgtggggtag attttggaca     840

aatctgtact ctttgacagt tccctttgga cagaaaccaa acatagatgt tactgatgca     900

atggtggacc aggcctggga tgcacagaga atattcaagg aggccgagaa gttctttgta     960

tctgttggtc ttcctaatat gactcaagga ttctgggaaa attccatgct aacggaccca    1020

ggaaatgttc agaaagcagt ctgccatccc acagcttggg acctggggaa gggcgacttc    1080

aggatcctta tgtgcacaaa ggtgacaatg gacgacttcc tgacagctca tcatgagatg    1140

gggcatatcc agtatgatat ggcatatgct gcacaacctt ttctgctaag aaatggagct    1200

aatgaaggat tccatgaagc tgttggggaa atcatgtcac tttctgcagc cacacctaag    1260

catttaaaat ccattggtct tctgtcaccc gattttcaag aagacaatga aacagaaata    1320

aacttcctgc tcaaacaagc actcacgatt gttgggactc tgccatttac ttacatgtta    1380

gagaagtgga ggtggatggt ctttaaaggg gaaattccca aagaccagtg gatgaaaaag    1440

tggtgggaga tgaagcgaga gatagttggg gtggtggaac ctgtgcccca tgatgaaaca    1500

tactgtgacc ccgcatctct gttccatgtt tctaatgatt actcattcat tcgatattac    1560

acaaggaccc tttaccaatt ccagtttcaa gaagcacttt gtcaagcagc taaacatgaa    1620

ggccctctgc acaaatgtga catctcaaac tctacagaag ctggacagaa actgttcaat    1680

atgctgaggc ttggaaaatc agaaccctgg accctagcat tggaaaatgt tgtaggagca    1740

aagaacatga atgtaaggcc actgctcaac tactttgagc ccttatttac ctggctgaaa    1800

gaccagaaca agaattcttt tgtgggatgg agtaccgact ggagtccata tgcagaccaa    1860

agcatcaaag tgaggataag cctaaaatca gctcttggag ataaagcata tgaatggaac    1920

gacaatgaaa tgtacctgtt ccgatcatct gttgcatatg ctatgaggca gtacttttta    1980

aaagtaaaaa atcagatgat tctttttggg gaggaggatg tgcgagtggc taatttgaaa    2040

ccaagaatct cctttaattt ctttgtcact gcacctaaaa atgtgtctga tatcattcct    2100

agaactgaag ttgaaaaggc catcaggatg tcccggagcc gtatcaatga tgctttccgt    2160

ctgaatgaca acagcctaga gtttctgggg atacagccaa cacttggacc tcctaaccag    2220

ccccctgttt ccatatggct gattgttttt ggagttgtga tgggagtgat agtggttggc    2280

attgtcatcc tgatcttcac tgggatcaga gatcggaaga agaaaaataa agcaagaagt    2340

ggagaaaatc cttatgcctc catcgatatt agcaaaggag aaaataatcc aggattccaa    2400

aacactgatg atgttcagac ctccttttaa ctcgag                              2436


<210> 6
<211> 1673
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of BamHI-XhoI DNA insert (Luciferase FireFly)"

<220> 
<223> Nucleotide sequence of BamHI-XhoI DNA insert (Luciferase FireFly)

<400> 6
ggatccgcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg      60

ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt     120

cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac     180

ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac     240

agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta     300

tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac     360

agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt     420

ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg     480

gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt     540

aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg     600

aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc     660

gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg     720

attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg     780

atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc     840

cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc     900

aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc     960

gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc    1020

aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat    1080

gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg    1140

gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg    1200

attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga    1260

tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac    1320

cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc    1380

atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac    1440

gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa    1500

gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt    1560

gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga    1620

gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaatgctc gag           1673


<210> 7
<211> 617
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of XhoI-KpnI DNA insert (WPREm)"

<220> 
<223> Nucleotide sequence of XhoI-KpnI DNA insert (WPREm)

<400> 7
ctcgagaatt cccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat      60

tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca     120

tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc     180

tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc     240

tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt     300

cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg     360

gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc     420

ctttccgcgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta     480

cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg     540

gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc     600

cccgcatcgg gggtacc                                                    617


<210> 8
<211> 3858
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of BamHI-XhoI DNA insert (Spike S High)"

<220> 
<223> Nucleotide sequence of BamHI-XhoI DNA insert (Spike S High)

<400> 8
ggatcccgta cggccaccat gttcgtgttt ctggtgctgc tgccactggt gtccagtcag       60

tgcgtgaacc tgaccacacg aacacagctg ccaccagcct acaccaatag cttcacccgc      120

ggagtgtact accccgacaa ggtgttccgc agcagcgtgc tgcatagcac ccaggatctg      180

tttctgccct tcttcagcaa cgtgacctgg ttccacgcca tccacgtgtc cggcaccaat      240

ggcaccaagc gcttcgataa tcccgtgctg cccttcaacg atggcgtgta ctttgccagc      300

accgagaagt ccaatatcat ccgcggctgg atcttcggca ccacactgga tagcaagacc      360

cagagcctgc tgatcgtgaa caacgccacc aacgtggtca tcaaagtgtg cgagttccag      420

ttctgcaacg accccttcct gggcgtctac taccacaaga acaacaagag ctggatggaa      480

agcgagttcc gcgtgtacag cagcgccaac aactgcacct tcgagtacgt gtcccagcca      540

ttcctgatgg acctggaagg caagcagggc aacttcaaga acctgcgcga gttcgtgttc      600

aagaacatcg acggctactt caaaatctac agcaagcaca ccccaatcaa cctcgtgcgc      660

gatctgccac agggattcag tgctctggaa cccctggtgg atctgcccat cggcatcaac      720

attacccgct ttcagacact gctggccctg caccgcagtt acttgacacc aggcgatagc      780

agcagtggat ggacagctgg tgccgccgct tactacgttg gatatctgca gccacgcacc      840

tttctgctga agtacaacga gaacggcacc atcaccgacg ccgtggattg tgctctcgat      900

cccctgagcg agacaaagtg caccctgaag tccttcaccg tcgagaaggg catctaccag      960

accagcaatt tccgcgtgca gcccaccgag agcatcgtgc gcttccccaa tatcaccaat     1020

ctgtgcccct tcggcgaggt gttcaatgcc acacgctttg cctccgtgta cgcctggaat     1080

cgcaagcgca ttagcaactg cgtggccgac tactccgtgc tgtacaatag cgccagcttc     1140

agcaccttca agtgctacgg cgtgtcaccc accaagctga acgacctgtg cttcaccaat     1200

gtgtacgccg acagcttcgt gatccgcgga gatgaagtgc gacagattgc cccaggccag     1260

accggcaaga tcgccgacta caattacaag ctgcccgacg acttcaccgg ctgcgtgatc     1320

gcctggaaca gcaacaacct ggattccaaa gtcggcggca actacaacta cctgtaccgc     1380

ctgttccgca agagcaatct gaagcccttc gagcgcgaca tcagcaccga aatctaccag     1440

gccggaagca ccccatgcaa cggcgtggaa ggcttcaact gctacttccc actgcagtcc     1500

tacggatttc agcccacaaa tggcgtgggc taccagccat atcgagtggt ggtgctgagc     1560

ttcgaactgc tgcatgctcc agctaccgtg tgcggcccca agaagagtac caacctggtc     1620

aagaacaaat gcgtgaactt caacttcaac ggcctgaccg gaaccggcgt gctgaccgag     1680

agtaacaaga agttcctgcc attccagcag tttggccgcg acattgccga tacaaccgat     1740

gccgttcgcg atccccagac cttggagatc ctggatatta ccccatgctc cttcggcggc     1800

gtgtccgtga ttacaccagg caccaatacc agcaaccagg tggccgttct gtaccaggat     1860

gtgaattgca cagaggtgcc cgtggccatt cacgccgatc aattgacacc aacatggcgc     1920

gtgtactcca ccggcagcaa tgtgtttcaa acccgcgctg gatgcctgat tggagccgag     1980

cacgtgaaca atagctacga gtgcgatatc cccatcggag ccggaatctg cgcctcctat     2040

cagacccaga ccaatagtcc acgacgagcc cgaagtgtgg ccagccagag catcattgcc     2100

tataccatga gcctgggcgc cgagaatagc gtggcctact ccaacaacag cattgctatc     2160

cccaccaact tcaccatcag cgtgaccacc gagatcctgc cagtgtccat gaccaagacc     2220

agcgtggact gcaccatgta catctgcgga gatagcaccg agtgcagcaa cctgctgctg     2280

cagtacggaa gtttctgcac ccagctgaat cgcgccctga caggcattgc cgtggaacag     2340

gataagaaca cccaagaggt gttcgcccaa gtgaagcaaa tctacaagac cccaccaatc     2400

aaggatttcg gcggcttcaa tttcagccag attctgcccg atccaagcaa gcccagcaag     2460

cgcagcttca tcgaggacct gctgttcaac aaagtgacac tggccgacgc cggattcatc     2520

aagcagtatg gcgattgcct gggcgatatt gccgcacgcg atctgatttg cgcccagaag     2580

tttaacggac tgaccgtcct gccaccactg ctgacagatg agatgatcgc ccagtacaca     2640

agtgccctgc tggccggaac cattaccagc ggatggacat ttggagccgg tgccgctctg     2700

cagattccct tcgctatgca gatggcctac cgcttcaatg gcattggcgt gacccagaat     2760

gtgctgtacg agaaccagaa gctgatcgcc aaccagttca acagcgccat cggcaagatt     2820

caggacagcc tgagtagtac cgccagcgct ctgggaaagc tgcaggatgt ggtcaaccag     2880

aacgctcagg ccctgaacac cctggttaag cagctgagca gcaacttcgg cgccatcagt     2940

agcgtgctga acgatatcct gagccgcctg gataaggtgg aagccgaggt gcagatcgat     3000

cgcctgatta ccggacgcct gcagtccctg cagacctatg tgacacagca gctgatccga     3060

gccgccgaga ttcgagctag tgctaatctg gccgccacca agatgagcga atgtgtgctg     3120

ggacagagca agcgcgtgga cttttgcggc aagggatacc acctgatgag cttcccacag     3180

agtgctccac acggcgtggt gtttctgcat gtgacctacg tgcccgctca agagaagaat     3240

ttcaccaccg ctccagccat ctgccacgac ggaaaggccc attttccacg cgagggcgtg     3300

ttcgttagca acggcactca ttggttcgtc acccagcgca acttctacga gccccagatc     3360

atcaccaccg acaacacctt cgtcagcggc aactgcgacg tcgtgatcgg cattgtgaac     3420

aacaccgtgt acgatccact gcagcccgag ctggacagct tcaaagagga actggacaag     3480

tactttaaga accacacaag ccccgacgtg gacctgggag acattagcgg aatcaacgcc     3540

agcgtggtca acatccagaa agagattgac cgcctgaacg aggtggccaa gaatctgaac     3600

gagagcctga tcgacctgca agaactgggc aaatacgagc agtacattaa gtggccctgg     3660

tacatctggc tgggcttcat tgccggactg attgccatcg tgatggtcac cattatgctg     3720

tgctgcatga ccagttgctg cagctgcctg aagggatgct gcagttgcgg aagctgctgc     3780

aagttcgacg aggatgatag cgagccagtg ctgaagggcg tcaagctgca ctacacctga     3840

taacgagcgc gcctcgag                                                   3858


<210> 9
<211> 795
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of Kanamycin"

<220> 
<223> Nucleotide sequence of Kanamycin

<400> 9
atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc      60

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     120

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     180

caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     240

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     300

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     360

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     420

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     480

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     540

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     600

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     660

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     720

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     780

gacgagttct tctga                                                      795


<210> 10
<211> 171
<212> PRT
<213> Artificial Sequence

<223> "Protein sequence of Nanoluc"

<220> 
<223> Protein sequence of Nanoluc

<400> 10
Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      
Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          
Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              
Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  
Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  
Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      
Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         
Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             
Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 
Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 
Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170     

<210> 11
<211> 590
<212> DNA
<213> Artificial Sequence

<223> "Nucleotide sequence of Nanolox"

<220> 
<223> Nucleotide sequence of Nanolox

<400> 11
ataacttcgt atagcataca ttatacgaag ttatgccacc atggtcttca cactcgaaga      60

tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg     120

aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt     180

cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct     240

gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga     300

tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa     360

catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat     420

cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc     480

cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga     540

acgcattctg gcgtaatatt gaagcatatc gtatgtaata tgcttcaata                590


<210> 12
<211> 34
<212> DNA
<213> Artificial Sequence

<223> "5' LOXP sequence"

<220> 
<223> 5' LOXP sequence

<400> 12
ataacttcgt atagcataca ttatacgaag ttat                                 34


<210> 13
<211> 34
<212> DNA
<213> Artificial Sequence

<223> "3' LOXP sequence"

<220> 
<223> 3' LOXP sequence

<400> 13
tattgaagca tatcgtatgt aatatgcttc aata                                 34


<210> 14
<211> 343
<212> PRT
<213> Artificial Sequence

<223> "Protein sequence of recombinase cre Phage P1"

<220> 
<223> Protein sequence of recombinase cre Phage P1

<400> 14
Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      
Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  
Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         
Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 
Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     
Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         
Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

