               SEQUENCE LISTING

<110> NEOVACS
      INSERM (Institut National de la Santé et de la Recherche
      Médicale)
      INSTITUT PASTEUR
      Reber Laurent
      Bruhns Pierre
      Conde Garcia Eva
      Backovic Marija
      Serra Vincent
      Géraldine GROUARD-VOGEL
      Romain BERTRAND
      
<120> IMMUNOGENIC PRODUCT COMPRISING AN IgE FRAGMENT FOR TREATING IgE-MEDIATED INFLAMMATORY DISORDERS

<130> IBIO-1656/PCT

<150> EP20306047.0
<151> 2020-09-17
<150> US63/079686
<151> 2020-09-17

<160> 15

<170> BiSSAP 1.3.6

<210> 1
<211> 535
<212> PRT
<213> Artificial Sequence


<220> 
<223> CRM197

<400> 1
Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      
Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          
Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              
Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  
Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  
Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      
Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         
Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             
Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 
Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 
Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     
Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         
Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             
Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 
Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 
Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     
His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         
Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             
Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 
Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 
Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     
Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         
Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             
Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 
Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 
Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     
Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         
Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             
His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 
Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 
Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     
Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         
Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 
        515                 520                 525             
Leu Phe Phe Glu Ile Lys Ser 
    530                 535 

<210> 2
<211> 428
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE constant region

<400> 2
Ala Ser Thr Gln Ser Pro Ser Val Phe Pro Leu Thr Arg Cys Cys Lys 
1               5                   10                  15      
Asn Ile Pro Ser Asn Ala Thr Ser Val Thr Leu Gly Cys Leu Ala Thr 
            20                  25                  30          
Gly Tyr Phe Pro Glu Pro Val Met Val Thr Trp Asp Thr Gly Ser Leu 
        35                  40                  45              
Asn Gly Thr Thr Met Thr Leu Pro Ala Thr Thr Leu Thr Leu Ser Gly 
    50                  55                  60                  
His Tyr Ala Thr Ile Ser Leu Leu Thr Val Ser Gly Ala Trp Ala Lys 
65                  70                  75                  80  
Gln Met Phe Thr Cys Arg Val Ala His Thr Pro Ser Ser Thr Asp Trp 
                85                  90                  95      
Val Asp Asn Lys Thr Phe Ser Val Cys Ser Arg Asp Phe Thr Pro Pro 
            100                 105                 110         
Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe Pro 
        115                 120                 125             
Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly Thr 
    130                 135                 140                 
Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp Leu 
145                 150                 155                 160 
Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln Ser 
                165                 170                 175     
Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr Thr 
            180                 185                 190         
Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys Lys 
        195                 200                 205             
Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro 
    210                 215                 220                 
Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu 
225                 230                 235                 240 
Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser 
                245                 250                 255     
Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys 
            260                 265                 270         
Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr 
        275                 280                 285             
Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro 
    290                 295                 300                 
His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro 
305                 310                 315                 320 
Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly 
                325                 330                 335     
Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro 
            340                 345                 350         
Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp 
        355                 360                 365             
Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe 
    370                 375                 380                 
Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys 
385                 390                 395                 400 
Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln 
                405                 410                 415     
Thr Val Gln Arg Ala Val Ser Val Asn Pro Gly Lys 
            420                 425             

<210> 3
<211> 105
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε3

<400> 3
Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu 
1               5                   10                  15      
Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala 
            20                  25                  30          
Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys 
        35                  40                  45              
Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr 
    50                  55                  60                  
Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu 
65                  70                  75                  80  
Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala 
                85                  90                  95      
Leu Met Arg Ser Thr Thr Lys Thr Ser 
            100                 105 

<210> 4
<211> 210
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε3 and Cε4

<400> 4
Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu 
1               5                   10                  15      
Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala 
            20                  25                  30          
Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys 
        35                  40                  45              
Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr 
    50                  55                  60                  
Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu 
65                  70                  75                  80  
Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala 
                85                  90                  95      
Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu 
            100                 105                 110         
Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg 
        115                 120                 125             
Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val 
    130                 135                 140                 
Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr 
145                 150                 155                 160 
Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg 
                165                 170                 175     
Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys 
            180                 185                 190         
Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala 
        195                 200                 205             
Val Ser 
    210 

<210> 5
<211> 207
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε2 and Cε3

<400> 5
Pro Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe 
1               5                   10                  15      
Pro Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly 
            20                  25                  30          
Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp 
        35                  40                  45              
Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln 
    50                  55                  60                  
Ser Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr 
65                  70                  75                  80  
Thr Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys 
                85                  90                  95      
Lys Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg 
            100                 105                 110         
Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys 
        115                 120                 125             
Leu Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp 
    130                 135                 140                 
Ser Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu 
145                 150                 155                 160 
Lys Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly 
                165                 170                 175     
Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His 
            180                 185                 190         
Pro His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser 
        195                 200                 205         

<210> 6
<211> 312
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε2, Cε3 and Cε4

<400> 6
Pro Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe 
1               5                   10                  15      
Pro Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly 
            20                  25                  30          
Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp 
        35                  40                  45              
Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln 
    50                  55                  60                  
Ser Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr 
65                  70                  75                  80  
Thr Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys 
                85                  90                  95      
Lys Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg 
            100                 105                 110         
Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys 
        115                 120                 125             
Leu Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp 
    130                 135                 140                 
Ser Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu 
145                 150                 155                 160 
Lys Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly 
                165                 170                 175     
Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His 
            180                 185                 190         
Pro His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly 
        195                 200                 205             
Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro 
    210                 215                 220                 
Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met 
225                 230                 235                 240 
Pro Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro 
                245                 250                 255     
Asp Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly 
            260                 265                 270         
Phe Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln 
        275                 280                 285             
Lys Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser 
    290                 295                 300                 
Gln Thr Val Gln Arg Ala Val Ser 
305                 310         

<210> 7
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide sequence + G335C

<400> 7
Ala Asp Ser Asn Pro Arg Cys Val Ser Ala Tyr Leu Ser Arg Pro Ser 
1               5                   10                  15      
Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val 
            20                  25                  30          
Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg 
        35                  40                  45              
Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln 
    50                  55                  60                  
Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg 
65                  70                  75                  80  
Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His 
                85                  90                  95      
Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro Arg 
            100                 105                 110         
Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly Ser 
        115                 120                 125             
Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro Glu 
    130                 135                 140                 
Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp Ala 
145                 150                 155                 160 
Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe Phe 
                165                 170                 175     
Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys Asp 
            180                 185                 190         
Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln Thr 
        195                 200                 205             
Val Gln Arg Ala Val Ser Val Asn Pro Gly Lys 
    210                 215                 

<210> 8
<211> 105
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε3 + G335C

<400> 8
Pro Arg Cys Val Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu 
1               5                   10                  15      
Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala 
            20                  25                  30          
Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys 
        35                  40                  45              
Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr 
    50                  55                  60                  
Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu 
65                  70                  75                  80  
Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala 
                85                  90                  95      
Leu Met Arg Ser Thr Thr Lys Thr Ser 
            100                 105 

<210> 9
<211> 210
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε3 and Cε4 + G335C

<400> 9
Pro Arg Cys Val Ser Ala Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu 
1               5                   10                  15      
Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val Val Asp Leu Ala 
            20                  25                  30          
Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys 
        35                  40                  45              
Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr 
    50                  55                  60                  
Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg Asp Trp Ile Glu 
65                  70                  75                  80  
Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His Leu Pro Arg Ala 
                85                  90                  95      
Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu 
            100                 105                 110         
Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg 
        115                 120                 125             
Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro Glu Asp Ile Ser Val 
    130                 135                 140                 
Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp Ala Arg His Ser Thr 
145                 150                 155                 160 
Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe Phe Val Phe Ser Arg 
                165                 170                 175     
Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys 
            180                 185                 190         
Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln Thr Val Gln Arg Ala 
        195                 200                 205             
Val Ser 
    210 

<210> 10
<211> 207
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε2 and Cε3 + G335C

<400> 10
Pro Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe 
1               5                   10                  15      
Pro Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly 
            20                  25                  30          
Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp 
        35                  40                  45              
Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln 
    50                  55                  60                  
Ser Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr 
65                  70                  75                  80  
Thr Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys 
                85                  90                  95      
Lys Cys Ala Asp Ser Asn Pro Arg Cys Val Ser Ala Tyr Leu Ser Arg 
            100                 105                 110         
Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys 
        115                 120                 125             
Leu Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp 
    130                 135                 140                 
Ser Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu 
145                 150                 155                 160 
Lys Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly 
                165                 170                 175     
Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His 
            180                 185                 190         
Pro His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser 
        195                 200                 205         

<210> 11
<211> 312
<212> PRT
<213> Artificial Sequence


<220> 
<223> hIgE Cε2, Cε3 and Cε4 + G335C

<400> 11
Pro Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly Gly Gly His Phe 
1               5                   10                  15      
Pro Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly Tyr Thr Pro Gly 
            20                  25                  30          
Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val Met Asp Val Asp 
        35                  40                  45              
Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu Ala Ser Thr Gln 
    50                  55                  60                  
Ser Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser Asp Arg Thr Tyr 
65                  70                  75                  80  
Thr Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu Asp Ser Thr Lys 
                85                  90                  95      
Lys Cys Ala Asp Ser Asn Pro Arg Cys Val Ser Ala Tyr Leu Ser Arg 
            100                 105                 110         
Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys 
        115                 120                 125             
Leu Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp 
    130                 135                 140                 
Ser Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu 
145                 150                 155                 160 
Lys Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly 
                165                 170                 175     
Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His 
            180                 185                 190         
Pro His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly 
        195                 200                 205             
Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro 
    210                 215                 220                 
Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met 
225                 230                 235                 240 
Pro Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro 
                245                 250                 255     
Asp Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly 
            260                 265                 270         
Phe Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln 
        275                 280                 285             
Lys Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser 
    290                 295                 300                 
Gln Thr Val Gln Arg Ala Val Ser 
305                 310         

<210> 12
<211> 219
<212> PRT
<213> Artificial Sequence


<220> 
<223> peptide sequence

<400> 12
Ala Asp Ser Asn Pro Arg Gly Val Ser Ala Tyr Leu Ser Arg Pro Ser 
1               5                   10                  15      
Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu Val 
            20                  25                  30          
Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser Arg 
        35                  40                  45              
Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys Gln 
    50                  55                  60                  
Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr Arg 
65                  70                  75                  80  
Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro His 
                85                  90                  95      
Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro Arg 
            100                 105                 110         
Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly Ser 
        115                 120                 125             
Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro Glu 
    130                 135                 140                 
Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp Ala 
145                 150                 155                 160 
Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe Phe 
                165                 170                 175     
Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys Asp 
            180                 185                 190         
Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln Thr 
        195                 200                 205             
Val Gln Arg Ala Val Ser Val Asn Pro Gly Lys 
    210                 215                 

<210> 13
<211> 419
<212> PRT
<213> Artificial Sequence


<220> 
<223> EIgE constant domain

<400> 13
Val Ser Lys Gln Ala Pro Leu Ile Phe Pro Leu Ala Ala Cys Cys Lys 
1               5                   10                  15      
Asp Thr Lys Thr Thr Asn Ile Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          
Phe Pro Gly Ala Trp Asp Ala Gly Pro Leu Asn Pro Ser Thr Met Thr 
        35                  40                  45              
Phe Pro Ala Val Phe Asp Gln Thr Ser Gly Leu Tyr Thr Thr Ile Ser 
    50                  55                  60                  
Arg Val Val Ala Ser Gly Lys Trp Ala Lys Gln Lys Phe Thr Cys Gly 
65                  70                  75                  80  
Val Val His Ser Gln Glu Thr Phe Asn Lys Thr Phe Asn Ala Cys Ile 
                85                  90                  95      
Val Thr Phe Thr Pro Pro Thr Val Lys Leu Phe His Ser Ser Cys Asp 
            100                 105                 110         
Pro Gly Gly Asp Ser His Thr Thr Ile Gln Leu Leu Cys Leu Ile Ser 
        115                 120                 125             
Asp Tyr Thr Pro Gly Asp Ile Asp Ile Val Trp Leu Ile Glu Gly Gln 
    130                 135                 140                 
Lys Val Asp Glu Gln Phe Pro Thr Gln Ala Ser Met Lys Gln Glu Gly 
145                 150                 155                 160 
Ser Trp Pro Pro Thr His Ser Glu Leu Asn Ile Asn Gln Gly Gln Trp 
                165                 170                 175     
Ala Ser Glu Asn Thr Tyr Thr Cys Gln Val Thr Tyr Lys Asp Met Ile 
            180                 185                 190         
Phe Asn Gln Ala Arg Lys Cys Thr Glu Ser Asp Pro Pro Gly Val Ser 
        195                 200                 205             
Val Tyr Leu Ser Pro Pro Ser Pro Leu Asp Leu Tyr Val Ser Lys Thr 
    210                 215                 220                 
Pro Lys Ile Thr Cys Leu Val Val Asp Leu Ala Asn Val Gln Gly Leu 
225                 230                 235                 240 
Ser Leu Asn Trp Ser Arg Glu Ser Gly Glu Pro Leu Gln Lys His Thr 
                245                 250                 255     
Leu Ala Thr Ser Glu Gln Phe Asn Lys Thr Phe Ser Val Thr Ser Thr 
            260                 265                 270         
Leu Pro Val Asp Thr Thr Asp Trp Ile Glu Gly Glu Thr Tyr Lys Cys 
        275                 280                 285             
Thr Val Ser His Pro Asp Leu Pro Arg Glu Val Val Arg Ser Ile Ala 
    290                 295                 300                 
Lys Ala Pro Gly Lys Arg Leu Ser Pro Glu Val Tyr Val Phe Leu Pro 
305                 310                 315                 320 
Pro Glu Glu Asp Gln Ser Ser Lys Asp Lys Val Thr Leu Thr Cys Leu 
                325                 330                 335     
Ile Gln Asn Phe Phe Pro Ala Asp Ile Ser Val Gln Trp Arg Arg Asn 
            340                 345                 350         
Asn Val Leu Ile Gln Thr Asp Gln Gln Ala Thr Thr Arg Pro Gln Lys 
        355                 360                 365             
Ala Asn Gly Pro Asp Pro Ala Phe Phe Val Phe Ser Arg Leu Glu Val 
    370                 375                 380                 
Ser Arg Ala Glu Trp Glu Gln Lys Asn Lys Phe Ala Cys Lys Val Val 
385                 390                 395                 400 
His Glu Ala Leu Ser Gln Arg Thr Leu Gln Lys Glu Val Ser Lys Asp 
                405                 410                 415     
Pro Gly Lys 
            

<210> 14
<211> 426
<212> PRT
<213> Artificial Sequence


<220> 
<223> cIgE constant domain

<400> 14
Thr Ser Gln Asp Leu Ser Val Phe Pro Leu Ala Ser Cys Cys Lys Asp 
1               5                   10                  15      
Asn Ile Ala Ser Thr Ser Val Thr Leu Gly Cys Leu Val Thr Gly Tyr 
            20                  25                  30          
Leu Pro Met Ser Thr Thr Val Thr Trp Asp Thr Gly Ser Leu Asn Lys 
        35                  40                  45              
Asn Val Thr Thr Phe Pro Thr Thr Phe His Glu Thr Tyr Gly Leu His 
    50                  55                  60                  
Ser Ile Val Ser Gln Val Thr Ala Ser Gly Lys Trp Ala Lys Gln Arg 
65                  70                  75                  80  
Phe Thr Cys Ser Val Ala His Ala Glu Ser Thr Ala Ile Asn Lys Thr 
                85                  90                  95      
Phe Ser Ala Cys Ala Leu Asn Phe Ile Pro Pro Thr Val Lys Leu Phe 
            100                 105                 110         
His Ser Ser Cys Asn Pro Val Gly Asp Thr His Thr Thr Ile Gln Leu 
        115                 120                 125             
Leu Cys Leu Ile Ser Gly Tyr Val Pro Gly Asp Met Glu Val Ile Trp 
    130                 135                 140                 
Leu Val Asp Gly Gln Lys Ala Thr Asn Ile Phe Pro Tyr Thr Ala Pro 
145                 150                 155                 160 
Gly Thr Lys Glu Gly Asn Val Thr Ser Thr His Ser Glu Leu Asn Ile 
                165                 170                 175     
Thr Gln Gly Glu Trp Val Ser Gln Lys Thr Tyr Thr Cys Gln Val Thr 
            180                 185                 190         
Tyr Gln Gly Phe Thr Phe Lys Asp Glu Ala Arg Lys Cys Ser Glu Ser 
        195                 200                 205             
Asp Pro Arg Gly Val Thr Ser Tyr Leu Ser Pro Pro Ser Pro Leu Asp 
    210                 215                 220                 
Leu Tyr Val His Lys Ala Pro Lys Ile Thr Cys Leu Val Val Asp Leu 
225                 230                 235                 240 
Ala Thr Met Glu Gly Met Asn Leu Thr Trp Tyr Arg Glu Ser Lys Glu 
                245                 250                 255     
Pro Val Asn Pro Gly Pro Leu Asn Lys Lys Asp His Phe Asn Gly Thr 
            260                 265                 270         
Ile Thr Val Thr Ser Thr Leu Pro Val Asn Thr Asn Asp Trp Ile Glu 
        275                 280                 285             
Gly Glu Thr Tyr Tyr Cys Arg Val Thr His Pro His Leu Pro Lys Asp 
    290                 295                 300                 
Ile Val Arg Ser Ile Ala Lys Ala Pro Gly Lys Arg Ala Pro Pro Asp 
305                 310                 315                 320 
Val Tyr Leu Phe Leu Pro Pro Glu Glu Glu Gln Gly Thr Lys Asp Arg 
                325                 330                 335     
Val Thr Leu Thr Cys Leu Ile Gln Asn Phe Phe Pro Ala Asp Ile Ser 
            340                 345                 350         
Val Gln Trp Leu Arg Asn Asp Ser Pro Ile Gln Thr Asp Gln Tyr Thr 
        355                 360                 365             
Thr Thr Gly Pro His Lys Val Ser Gly Ser Arg Pro Ala Phe Phe Ile 
    370                 375                 380                 
Phe Ser Arg Leu Glu Val Ser Arg Val Asp Trp Glu Gln Lys Asn Lys 
385                 390                 395                 400 
Phe Thr Cys Gln Val Val His Glu Ala Leu Ser Gly Ser Arg Ile Leu 
                405                 410                 415     
Gln Lys Trp Val Ser Lys Thr Pro Gly Lys 
            420                 425     

<210> 15
<211> 496
<212> PRT
<213> Artificial Sequence


<220> 
<223> fIgE constant domain

<400> 15
Ala Tyr Ile Ser Ser Gly Gly Asn Thr Asp Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      
Gly Arg Phe Ser Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
            20                  25                  30          
Gln Met Thr Ser Leu Lys Thr Glu Asp Thr Ala Thr Tyr Tyr Cys Ala 
        35                  40                  45              
Arg Gly Thr Gly Val Ile Pro Asp Tyr Trp Gly Gln Gly Ala Leu Val 
    50                  55                  60                  
Thr Val Ser Ser Thr Ser Ile Gln Ala Pro Leu Val Phe Pro Leu Ala 
65                  70                  75                  80  
Thr Cys Cys Lys Gly Thr Ile Ala Thr Ala Pro Ser Val Thr Leu Gly 
                85                  90                  95      
Cys Leu Val Thr Gly Tyr Phe Pro Met Pro Val Thr Val Thr Trp Asp 
            100                 105                 110         
Ala Arg Ser Leu Asn Lys Ser Val Val Thr Leu Pro Ala Thr Leu Gln 
        115                 120                 125             
Glu Asn Ser Gly Leu Tyr Thr Thr Thr Ser His Val Thr Val Ser Gly 
    130                 135                 140                 
Glu Trp Ala Lys Gln Lys Phe Thr Cys Ser Val Ala His Ala Glu Ser 
145                 150                 155                 160 
Pro Thr Ile Asn Lys Thr Val Ser Ala Cys Thr Met Asn Phe Ile Pro 
                165                 170                 175     
Pro Thr Val Lys Leu Phe His Ser Ser Cys Asn Pro Leu Gly Asp Thr 
            180                 185                 190         
Gly Ser Thr Ile Gln Leu Leu Cys Leu Ile Ser Gly Tyr Val Pro Gly 
        195                 200                 205             
Asp Met Glu Val Thr Trp Leu Val Asp Gly Gln Lys Ala Thr Asn Ile 
    210                 215                 220                 
Phe Pro Tyr Thr Ala Pro Gly Lys Gln Glu Gly Lys Val Thr Ser Thr 
225                 230                 235                 240 
His Ser Glu Leu Asn Ile Thr Gln Gly Glu Trp Val Ser Gln Lys Thr 
                245                 250                 255     
Tyr Thr Cys Gln Val Thr Tyr Gln Gly Phe Thr Phe Glu Asp His Ala 
            260                 265                 270         
Arg Lys Cys Thr Glu Ser Asp Pro Arg Gly Val Ser Thr Tyr Leu Ser 
        275                 280                 285             
Pro Pro Ser Pro Leu Asp Leu Tyr Val His Lys Ser Pro Lys Ile Thr 
    290                 295                 300                 
Cys Leu Val Val Asp Leu Ala Asn Thr Asp Gly Met Ile Leu Thr Trp 
305                 310                 315                 320 
Ser Arg Glu Asn Gly Glu Ser Val His Pro Asp Pro Met Val Lys Lys 
                325                 330                 335     
Thr Gln Tyr Asn Gly Thr Ile Thr Val Thr Ser Thr Leu Pro Val Asp 
            340                 345                 350         
Ala Thr Asp Trp Val Glu Gly Glu Thr Tyr Gln Cys Lys Val Thr His 
        355                 360                 365             
Pro Asp Leu Pro Lys Asp Ile Val Arg Ser Ile Ala Lys Ala Pro Gly 
    370                 375                 380                 
Arg Arg Phe Pro Pro Glu Val Tyr Val Phe Leu Pro Pro Glu Gly Glu 
385                 390                 395                 400 
Pro Lys Thr Lys Asp Lys Val Thr Leu Thr Cys Leu Ile Gln Asn Phe 
                405                 410                 415     
Phe Pro Pro Asp Ile Ser Val Gln Trp Leu His Asn Asp Ser Pro Val 
            420                 425                 430         
Arg Thr Glu Gln Gln Ala Thr Thr Trp Pro His Lys Ala Thr Gly Pro 
        435                 440                 445             
Ser Pro Ala Phe Phe Val Phe Ser Arg Leu Glu Val Ser Arg Ala Asp 
    450                 455                 460                 
Trp Glu Gln Arg Asp Val Phe Thr Cys Gln Val Val His Glu Ala Leu 
465                 470                 475                 480 
Pro Gly Phe Arg Thr Leu Lys Lys Ser Val Ser Lys Asn Pro Gly Lys 
                485                 490                 495     



