
                                SEQUENCE LISTING

<110> Mark S. CHEE

<120> METHODS AND COMPOSITIONS FOR NUCLEIC
      ACID ASSEMBLY

<130> 71092-20001.40

<140> PCT/US2021/050126   
<141> 2021-09-13  

<150> US 63/078,178       
<151> 2020-09-14  

<160> 108

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
<223> n = A,T,C or G

<400> 1
ctgaagnnnn nnnnnnnnnn nn                                          22

<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 2
nnnnnnnnnn nnnncttcag                                             20

<210> 3
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9
<223> n = A,T,C or G

<400> 3
ggatcnnnn                                                         9

<210> 4
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 4
nnnnngatcc                                                        10

<210> 5
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 14, 15, 16, 22, 23, 24,
25, 26, 27, 28, 29, 30, 31, 32, 33
<223> n = A,T,C or G

<400> 5
nnnnnnnnnn acnnnngtay cnnnnnnnnn nnn                              33

<210> 6
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 13, 14, 15, 16, 19, 20, 21, 22, 23,
24, 25, 26, 27, 28, 29, 30, 31, 32, 33
<223> n = A,T,C or G

<400> 6
nnnnnnngrt acnnnngtnn nnnnnnnnnn nnn                              33

<210> 7
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8
<223> n = A,T,C or G

<400> 7
gaagacnn                                                          8

<210> 8
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6
<223> n = A,T,C or G

<400> 8
nnnnnngtct tc                                                     12

<210> 9
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8
<223> n = A,T,C or G

<400> 9
gaagacnn                                                          8

<210> 10
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6
<223> n = A,T,C or G

<400> 10
nnnnnngtct tc                                                     12

<210> 11
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13
<223> n = A,T,C or G

<400> 11
gcagcnnnnn nnn                                                    13

<210> 12
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
<223> n = A,T,C or G

<400> 12
nnnnnnnnnn nngctgc                                                17

<210> 13
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9
<223> n = A,T,C or G

<400> 13
ccatcnnnn                                                         9

<210> 14
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 14
nnnnngatgg                                                        10

<210> 15
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
<223> n = A,T,C or G

<400> 15
acggcnnnnn nnnnnnn                                                17

<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 16
nnnnnnnnnn nnnngccgt                                              19

<210> 17
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 14, 15, 16, 17, 18, 19, 23,
24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
<223> n = A,T,C or G

<400> 17
nnnnnnnnnn cgannnnnnt gcnnnnnnnn nnnn                             34

<210> 18
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 14, 15, 16, 17, 18, 19, 23,
24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
<223> n = A,T,C or G

<400> 18
nnnnnnnnnn gcannnnnnt cgnnnnnnnn nnnn                             34

<210> 19
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12
<223> n = A,T,C or G

<400> 19
gtatccnnnn nn                                                     12

<210> 20
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 20
nnnnnggata c                                                      11

<210> 21
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6
<223> n = A,T,C or G

<400> 21
gtctcn                                                            6

<210> 22
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 22
nnnnngagac                                                        10

<210> 23
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11
<223> n = A,T,C or G

<400> 23
actgggnnnn n                                                      11

<210> 24
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4
<223> n = A,T,C or G

<400> 24
nnnncccagt                                                        10

<210> 25
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10
<223> n = A,T,C or G

<400> 25
acctgcnnnn                                                        10

<210> 26
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8
<223> n = A,T,C or G

<400> 26
nnnnnnnngc aggt                                                   14

<210> 27
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11
<223> n = A,T,C or G

<400> 27
actgggnnnn n                                                      11

<210> 28
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4
<223> n = A,T,C or G

<400> 28
nnnncccagt                                                        10

<210> 29
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
<223> n = A,T,C or G

<400> 29
ctggagnnnn nnnnnnnnnn nn                                          22

<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 30
nnnnnnnnnn nnnnctccag                                             20

<210> 31
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
<223> n = A,T,C or G

<400> 31
cttgagnnnn nnnnnnnnnn nn                                          22

<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 32
nnnnnnnnnn nnnnctcaag                                             20

<210> 33
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 33
ggtctcn                                                           7

<210> 34
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 34
nnnnngagac c                                                      11

<210> 35
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 35
ggtctcn                                                           7

<210> 36
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 36
nnnnngagac c                                                      11

<210> 37
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, 15, 16, 21, 22, 23,
24, 25, 26, 27, 28, 29, 30
<223> n = A,T,C or G

<400> 37
nnnnnnnnna cnnnnnctcc nnnnnnnnnn                                  30

<210> 38
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 12, 13, 14, 15, 16, 19, 20, 21, 22,
23, 24, 25, 26, 27, 28, 29, 30
<223> n = A,T,C or G

<400> 38
nnnnnnngga gnnnnngtnn nnnnnnnnnn                                  30

<210> 39
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
<223> n = A,T,C or G

<400> 39
gaggagnnnn nnnnnn                                                 16

<210> 40
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8
<223> n = A,T,C or G

<400> 40
nnnnnnnnct cctc                                                   14

<210> 41
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
<223> n = A,T,C or G

<400> 41
gtgcagnnnn nnnnnnnnnn nn                                          22

<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 42
nnnnnnnnnn nnnnctgcac                                             20

<210> 43
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6
<223> n = A,T,C or G

<400> 43
gtctcn                                                            6

<210> 44
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 44
nnnnngagac                                                        10

<210> 45
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 45
cgtctcn                                                           7

<210> 46
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 46
nnnnngagac g                                                      11

<210> 47
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 47
cgtctcn                                                           7

<210> 48
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 48
nnnnngagac g                                                      11

<210> 49
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
<223> n = A,T,C or G

<400> 49
gggacnnnnn nnnnn                                                  15

<210> 50
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 50
nnnnnnnnnn nnnngtccc                                              19

<210> 51
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 51
gaatgcn                                                           7

<210> 52
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1
<223> n = A,T,C or G

<400> 52
ngcattc                                                           7

<210> 53
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 53
ctcagnnnnn nnnn                                                   14

<210> 54
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7
<223> n = A,T,C or G

<400> 54
nnnnnnnctg ag                                                     12

<210> 55
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10
<223> n = A,T,C or G

<400> 55
acctgcnnnn                                                        10

<210> 56
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7
<223> n = A,T,C or G

<400> 56
nnnnnnngca ggt                                                    13

<210> 57
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 8
<223> n = A,T,C or G

<400> 57
gctcttcn                                                          8

<210> 58
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4
<223> n = A,T,C or G

<400> 58
nnnngaagag c                                                      11

<210> 59
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8
<223> n = A,T,C or G

<400> 59
gcaatgnn                                                          8

<210> 60
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 60
cattgc                                                            6

<210> 61
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6
<223> n = A,T,C or G

<400> 61
actggn                                                            6

<210> 62
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1
<223> n = A,T,C or G

<400> 62
nccagt                                                            6

<210> 63
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7
<223> n = A,T,C or G

<400> 63
ggatgnn                                                           7

<210> 64
<211> 5
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 64
catcc                                                             5

<210> 65
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
<223> n = A,T,C or G

<400> 65
gcgatgnnnn nnnnnn                                                 16

<210> 66
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 66
nnnnnnnnnn nnnncatcgc                                             20

<210> 67
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8
<223> n = A,T,C or G

<400> 67
gcagtgnn                                                          8

<210> 68
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 68
cactgc                                                            6

<210> 69
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7
<223> n = A,T,C or G

<400> 69
ggatgnn                                                           7

<210> 70
<211> 5
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 70
catcc                                                             5

<210> 71
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8
<223> n = A,T,C or G

<400> 71
gcagtgnn                                                          8

<210> 72
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 72
cactgc                                                            6

<210> 73
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7
<223> n = A,T,C or G

<400> 73
cagtgnn                                                           7

<210> 74
<211> 5
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<400> 74
cactg                                                             5

<210> 75
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15, 16, 17, 18, 19, 24,
25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
<223> n = A,T,C or G

<400> 75
nnnnnnnnnn ncaannnnng tggnnnnnnn nnnnn                            35

<210> 76
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 16, 17, 18, 19, 23, 24,
25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
<223> n = A,T,C or G

<400> 76
nnnnnnnnnn ccacnnnnnt tgnnnnnnnn nnnnn                            35

<210> 77
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 77
ctcttcn                                                           7

<210> 78
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4
<223> n = A,T,C or G

<400> 78
nnnngaagag                                                        10

<210> 79
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
<223> n = A,T,C or G

<400> 79
ggcggannnn nnnnnnn                                                17

<210> 80
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9
<223> n = A,T,C or G

<400> 80
nnnnnnnnnt ccgcc                                                  15

<210> 81
<211> 7
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7
<223> n = A,T,C or G

<400> 81
cgtctcn                                                           7

<210> 82
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 82
nnnnngagac g                                                      11

<210> 83
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9
<223> n = A,T,C or G

<400> 83
cccgcnnnn                                                         9

<210> 84
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6
<223> n = A,T,C or G

<400> 84
nnnnnngcgg g                                                      11

<210> 85
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13, 14
<223> n = A,T,C or G

<400> 85
ggatgnnnnn nnnn                                                   14

<210> 86
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
<223> n = A,T,C or G

<400> 86
nnnnnnnnnn nnncatcc                                               18

<210> 87
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10
<223> n = A,T,C or G

<400> 87
gacgcnnnnn                                                        10

<210> 88
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
<223> n = A,T,C or G

<400> 88
nnnnnnnnnn gcgtc                                                  15

<210> 89
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13
<223> n = A,T,C or G

<400> 89
ggtgannnnn nnn                                                    13

<210> 90
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7
<223> n = A,T,C or G

<400> 90
nnnnnnntca cc                                                     12

<210> 91
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11
<223> n = A,T,C or G

<400> 91
ccttcnnnnn n                                                      11

<210> 92
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 92
nnnnngaagg                                                        10

<210> 93
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10, 11, 12, 13
<223> n = A,T,C or G

<400> 93
gaagannnnn nnn                                                    13

<210> 94
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7
<223> n = A,T,C or G

<400> 94
nnnnnnntct tc                                                     12

<210> 95
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10
<223> n = A,T,C or G

<400> 95
gagtcnnnnn                                                        10

<210> 96
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 96
nnnnngactc                                                        10

<210> 97
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,
22, 23, 24, 25, 26
<223> n = A,T,C or G

<400> 97
tccracnnnn nnnnnnnnnn nnnnnn                                      26

<210> 98
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,
18
<223> n = A,T,C or G

<400> 98
nnnnnnnnnn nnnnnnnngt ygga                                        24

<210> 99
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 5, 6, 7, 8, 9, 10, 11
<223> n = A,T,C or G

<400> 99
cctcnnnnnn n                                                      11

<210> 100
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6
<223> n = A,T,C or G

<400> 100
nnnnnngagg                                                        10

<210> 101
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,
22, 23, 24, 25, 26, 27
<223> n = A,T,C or G

<400> 101
gccgagnnnn nnnnnnnnnn nnnnnnn                                     27

<210> 102
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,
18, 19
<223> n = A,T,C or G

<400> 102
nnnnnnnnnn nnnnnnnnnc tcggc                                       25

<210> 103
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9
<223> n = A,T,C or G

<400> 103
gagtcnnnn                                                         9

<210> 104
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5
<223> n = A,T,C or G

<400> 104
nnnnngactc                                                        10

<210> 105
<211> 8
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 8
<223> n = A,T,C or G

<400> 105
gctcttcn                                                          8

<210> 106
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4
<223> n = A,T,C or G

<400> 106
nnnngaagag c                                                      11

<210> 107
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 6, 7, 8, 9, 10
<223> n = A,T,C or G

<400> 107
gcatcnnnnn                                                        10

<210> 108
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic construct

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9
<223> n = A,T,C or G

<400> 108
nnnnnnnnng atgc                                                   14
