                         SEQUENCE LISTING

<110>  Artizan Biosciences, Inc.
 
<120>  SMALL MOLECULE INHIBITORS OF BACTERIAL TOXINS

<130>  ARTI-006/01WO 329345-2060

<150>  US 63/077,354
<151>  2020-09-11

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1275
<212>  DNA
<213>  Bacteroides fragilis

<400>  1
gcgcgattac tagcgaatcc agcttcacga agtcgggttg cagacttcga tccgaactga       60

gagaggattt tgggattagc atacggtcac ccgctagctg ccttctgtac cccccattgt      120

aacacgtgtg tagccccgga cgtaagggcc gtgctgattt gacgtcatcc ccaccttcct      180

cacatcttac gacggcagtc tctccagagt cctcagcatg acctgttagt aactgaagat      240

aagggttgcg ctcgttatgg cacttaagcc gacacctcac ggcacgagct gacgacaacc      300

atgcagcacc ttcacagcgg tgattgctca ctgacatgtt tccacatcat tccactgcaa      360

tttaagcccg ggtaaggttc ctcgcgtatc atcgaattaa accacatgtt cctccgcttg      420

tgcgggcccc cgtcaattcc tttgagtttc accgttgccg gcgtactccc caggtggaat      480

acttaatgct ttcgcttggc cgcttactgt atatcgcaaa cagcgagtat tcatcgttta      540

ctgtgtggac taccagggta tctaatcctg tttgataccc acactttcga gcatcagtgt      600

cagttgcagt ccagtgagct gccttcgcaa tcggagttct tcgtgatatc taagcatttc      660

accgctacac cacgaattcc gcccacctct actgtactca agactgacag tatcaactgc      720

aattttacgg ttgagccgca ractttcaca actgacttac cagtccacct acgctccctt      780

taaacccaat aaatccggat aacgctcgga tcctccgtat taccgcggct gctggcacgg      840

agttagccga tccttattca tataatacat acaaaacagt atacatactg cactttattc      900

ttatataaaa gaagtttacg acccatagag ccttcatcct tcacgctact tggctggttc      960

aggctagcgc ccattgacca atattcctca ctgctgcctc ccgtaggagt ttggaccgtg     1020

tctcagttcc aatgtggggg accttcctct cagaacccct atccatcgaa ggcttggtga     1080

gccgttacct caccaacaac ctaatggaac gcatccccat cctttaccgg aatcctttaa     1140

taatgaaacc atgcggaatc attatgctat cgggtattaa tctttctttc gaaaggctat     1200

ccccgagtaa agggcaggtt ggatacgtgt tactcacccg tgcgccggtc gccggcaaag     1260

aaagcaagct ttctt                                                      1275


<210>  2
<211>  405
<212>  PRT
<213>  Bacteroides fragilis

<400>  2

Met Phe Ile Leu Asn Phe Asn Lys Met Lys Asn Val Lys Leu Leu Leu 
1               5                   10                  15      


Met Leu Gly Thr Ala Ala Leu Leu Ala Ala Cys Ser Asn Glu Ala Asp 
            20                  25                  30          


Ser Leu Thr Thr Ser Ile Asp Ala Pro Val Thr Ala Ser Ile Asp Leu 
        35                  40                  45              


Gln Ser Val Ser Tyr Thr Asp Leu Ala Thr Gln Leu Asn Asp Val Ser 
    50                  55                  60                  


Asp Phe Gly Lys Met Ile Ile Leu Lys Asp Asn Gly Phe Asn Arg Gln 
65                  70                  75                  80  


Val His Val Ser Met Asp Lys Arg Thr Lys Ile Gln Leu Asp Asn Glu 
                85                  90                  95      


Asn Val Arg Leu Phe Asn Gly Arg Asp Lys Asp Ser Thr Ser Phe Ile 
            100                 105                 110         


Leu Gly Asp Glu Phe Ala Val Leu Arg Phe Tyr Arg Asn Gly Glu Ser 
        115                 120                 125             


Ile Ser Tyr Ile Ala Tyr Lys Glu Ala Gln Met Met Asn Glu Ile Ala 
    130                 135                 140                 


Glu Phe Tyr Ala Ala Pro Phe Lys Lys Thr Arg Ala Ile Asn Glu Lys 
145                 150                 155                 160 


Glu Ala Phe Glu Cys Ile Tyr Asp Ser Arg Thr Arg Ser Ala Gly Lys 
                165                 170                 175     


Asp Ile Val Ser Val Lys Ile Asn Ile Asp Lys Ala Lys Lys Ile Leu 
            180                 185                 190         


Asn Leu Pro Glu Cys Asp Tyr Ile Asn Asp Tyr Ile Lys Thr Pro Gln 
        195                 200                 205             


Val Pro His Gly Ile Thr Glu Ser Gln Thr Arg Ala Val Pro Ser Glu 
    210                 215                 220                 


Pro Lys Thr Val Tyr Val Ile Cys Leu Arg Glu Asn Gly Ser Thr Ile 
225                 230                 235                 240 


Tyr Pro Asn Glu Val Ser Ala Gln Met Gln Asp Ala Ala Asn Ser Val 
                245                 250                 255     


Tyr Ala Val His Gly Leu Lys Arg Tyr Val Asn Phe His Phe Val Leu 
            260                 265                 270         


Tyr Thr Thr Glu Tyr Ser Cys Pro Ser Gly Asp Ala Lys Glu Gly Leu 
        275                 280                 285             


Glu Gly Phe Thr Ala Ser Leu Lys Ser Asn Pro Lys Ala Glu Gly Tyr 
    290                 295                 300                 


Asp Asp Gln Ile Tyr Phe Leu Ile Arg Trp Gly Thr Trp Asp Asn Lys 
305                 310                 315                 320 


Ile Leu Gly Met Ser Trp Phe Asn Ser Tyr Asn Val Asn Thr Ala Ser 
                325                 330                 335     


Asp Phe Glu Ala Ser Gly Met Ser Thr Thr Gln Leu Met Tyr Pro Gly 
            340                 345                 350         


Val Met Ala His Glu Leu Gly His Ile Leu Gly Ala Glu His Thr Asp 
        355                 360                 365             


Asn Ser Lys Asp Leu Met Tyr Ala Thr Phe Thr Gly Tyr Leu Ser His 
    370                 375                 380                 


Leu Ser Glu Lys Asn Met Asp Ile Ile Ala Lys Asn Leu Gly Trp Glu 
385                 390                 395                 400 


Ala Ala Asp Gly Asp 
                405 


<210>  3
<211>  397
<212>  PRT
<213>  Bacteroides fragilis

<400>  3

Met Lys Asn Val Lys Leu Leu Leu Met Leu Gly Thr Ala Ala Leu Leu 
1               5                   10                  15      


Ala Ala Cys Ser Asn Glu Ala Asp Ser Leu Thr Thr Ser Ile Asp Thr 
            20                  25                  30          


Pro Val Thr Ala Ser Ile Asp Leu Gln Ser Val Ser Tyr Thr Asp Leu 
        35                  40                  45              


Ala Thr Gln Leu Asn Asp Val Ser Asp Phe Gly Lys Met Ile Ile Leu 
    50                  55                  60                  


Lys Asp Asn Gly Phe Asn Arg Gln Val His Val Ser Met Asp Lys Arg 
65                  70                  75                  80  


Thr Lys Ile Gln Leu Asp Asn Glu Asn Val Arg Leu Phe Asn Gly Arg 
                85                  90                  95      


Asp Lys Asp Ser Thr Ser Phe Ile Leu Gly Asp Glu Phe Ala Val Leu 
            100                 105                 110         


Arg Phe Tyr Arg Asn Gly Glu Ser Ile Ser Tyr Ile Ala Tyr Lys Glu 
        115                 120                 125             


Ala Gln Met Met Asn Glu Ile Ala Glu Phe Tyr Ala Ala Pro Phe Lys 
    130                 135                 140                 


Lys Thr Arg Ala Ile Asn Glu Lys Glu Ala Phe Glu Cys Ile Tyr Asp 
145                 150                 155                 160 


Ser Arg Thr Arg Ser Ala Gly Lys Asp Leu Val Ser Val Lys Ile Asn 
                165                 170                 175     


Ile Asp Lys Ala Lys Lys Ile Leu Asn Leu Pro Glu Cys Asp Tyr Ile 
            180                 185                 190         


Asn Asp Tyr Ile Lys Thr Pro Gln Val Pro His Gly Ile Thr Glu Ser 
        195                 200                 205             


Gln Thr Arg Ala Val Pro Ser Glu Pro Lys Thr Val Tyr Val Ile Cys 
    210                 215                 220                 


Leu Arg Glu Ser Gly Ser Thr Val Tyr Pro Asn Glu Val Ser Ala Gln 
225                 230                 235                 240 


Met Gln Asp Ala Ala Asn Ser Val Tyr Ala Val His Gly Leu Lys Arg 
                245                 250                 255     


Phe Val Asn Leu His Phe Val Leu Tyr Thr Thr Glu Tyr Ser Cys Pro 
            260                 265                 270         


Ser Gly Asn Ala Asp Glu Gly Leu Asp Gly Phe Thr Ala Ser Leu Lys 
        275                 280                 285             


Ala Asn Pro Lys Ala Glu Gly Tyr Asp Asp Gln Ile Tyr Phe Leu Ile 
    290                 295                 300                 


Arg Trp Gly Thr Trp Asp Asn Asn Ile Leu Gly Ile Ser Trp Leu Asp 
305                 310                 315                 320 


Ser Tyr Asn Val Asn Thr Ala Ser Asp Phe Lys Ala Ser Gly Met Ser 
                325                 330                 335     


Thr Thr Gln Leu Met Tyr Pro Gly Val Met Ala His Glu Leu Gly His 
            340                 345                 350         


Ile Leu Gly Ala Arg His Ala Asp Asp Pro Lys Asp Leu Met Tyr Ser 
        355                 360                 365             


Lys Tyr Thr Gly Tyr Leu Phe His Leu Ser Glu Glu Asn Met Tyr Arg 
    370                 375                 380                 


Ile Ala Lys Asn Leu Gly Trp Glu Ile Ala Asp Gly Asp 
385                 390                 395         


<210>  4
<211>  397
<212>  PRT
<213>  Bacteroides fragilis

<400>  4

Met Lys Asn Val Lys Leu Leu Leu Met Leu Gly Thr Ala Ala Leu Leu 
1               5                   10                  15      


Ala Ala Cys Ser Asn Glu Ala Asp Ser Leu Thr Thr Ser Ile Asp Ala 
            20                  25                  30          


Pro Val Thr Ala Ser Ile Asp Leu Gln Ser Val Ser Tyr Thr Asp Leu 
        35                  40                  45              


Ala Thr Gln Leu Asn Asp Val Ser Asp Phe Gly Lys Met Ile Ile Leu 
    50                  55                  60                  


Lys Asp Asn Gly Phe Asn Arg Gln Val His Val Ser Met Asp Lys Arg 
65                  70                  75                  80  


Thr Lys Ile Gln Leu Asp Asn Glu Asn Val Arg Leu Phe Asn Gly Arg 
                85                  90                  95      


Asp Lys Asp Ser Thr Asn Phe Ile Leu Gly Asp Glu Phe Ala Val Leu 
            100                 105                 110         


Arg Phe Tyr Arg Asn Gly Glu Ser Ile Ser Tyr Ile Ala Tyr Lys Glu 
        115                 120                 125             


Ala Gln Met Met Asn Glu Ile Ala Glu Phe Tyr Ala Ala Pro Phe Lys 
    130                 135                 140                 


Lys Thr Arg Ala Ile Asn Glu Lys Glu Ala Phe Glu Cys Ile Tyr Asp 
145                 150                 155                 160 


Ser Arg Thr Arg Ser Ala Gly Lys Tyr Pro Val Ser Val Lys Ile Asn 
                165                 170                 175     


Val Asp Lys Ala Lys Lys Ile Leu Asn Leu Pro Glu Cys Asp Tyr Ile 
            180                 185                 190         


Asn Asp Tyr Ile Lys Thr Pro Gln Val Pro His Gly Ile Thr Glu Ser 
        195                 200                 205             


Gln Thr Arg Ala Val Pro Ser Glu Pro Lys Thr Val Tyr Val Ile Cys 
    210                 215                 220                 


Leu Arg Glu Asn Gly Ser Thr Val Tyr Pro Asn Glu Val Ser Ala Gln 
225                 230                 235                 240 


Met Gln Asp Ala Ala Asn Ser Val Tyr Ala Val His Gly Leu Lys Arg 
                245                 250                 255     


Tyr Val Asn Leu His Phe Val Leu Tyr Thr Thr Glu Tyr Ala Cys Pro 
            260                 265                 270         


Ser Gly Asn Ala Asp Glu Gly Leu Asp Gly Phe Thr Ala Ser Leu Lys 
        275                 280                 285             


Ala Asn Pro Lys Ala Glu Gly Tyr Asp Asp Gln Ile Tyr Phe Leu Ile 
    290                 295                 300                 


Arg Trp Gly Thr Trp Asp Asn Asn Ile Leu Gly Ile Ser Trp Leu Asn 
305                 310                 315                 320 


Ser Tyr Asn Val Asn Thr Ala Ser Asp Phe Lys Ala Ser Gly Met Ser 
                325                 330                 335     


Thr Thr Gln Leu Met Tyr Pro Gly Val Met Ala His Glu Leu Gly His 
            340                 345                 350         


Ile Leu Gly Ala Asn His Ala Asp Asp Pro Lys Asp Leu Met Tyr Ser 
        355                 360                 365             


Lys Tyr Thr Gly Tyr Leu Phe His Leu Ser Glu Lys Asn Met Asp Ile 
    370                 375                 380                 


Ile Ala Lys Asn Leu Gly Trp Glu Ile Ala Asp Gly Asp 
385                 390                 395         


<210>  5
<211>  1478
<212>  DNA
<213>  Enterococcus faecalis


<220>
<221>  misc_feature
<222>  (468)..(468)
<223>  n is any nucleotide

<400>  5
gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttctttcc tcccgagtgc       60

ttgcactcaa ttggaaagag gagtggcgga cgggtgagta acacgtgggt aacctaccca      120

tcagaggggg ataacacttg gaaacaggtg ctaataccgc ataacagttt atgccgcatg      180

gcataagagt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct      240

agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg      300

gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta gggaatcttc      360

ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt      420

aaaactctgt tgttagagaa gaacaaggac gttagtaact gaacgtcncc tgacggtatc      480

taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc      540

gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa      600

gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag      660

agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa      720

ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt      780

agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc      840

ttcagtgctg cagcaaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa      900

actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca      960

acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagctttccc     1020

ttcggggaca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg     1080

gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc     1140

tagcgagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc     1200

cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc tagaccgcga     1260

ggtcatgcaa atctcttaaa gcttctctca gttcggattg caggctgcaa ctcgcctgca     1320

tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc     1380

ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc     1440

tttttggagc cagccgccta aggtgggata gatgattg                             1478


<210>  6
<211>  510
<212>  PRT
<213>  Enterococcus faecalis

<400>  6

Met Met Lys Gly Asn Lys Ile Leu Tyr Ile Leu Gly Thr Gly Ile Phe 
1               5                   10                  15      


Val Gly Ser Ser Cys Leu Phe Ser Ser Leu Phe Val Ala Ala Glu Glu 
            20                  25                  30          


Gln Val Tyr Ser Glu Ser Glu Val Ser Thr Val Leu Ser Lys Leu Glu 
        35                  40                  45              


Lys Glu Ala Ile Ser Glu Ala Ala Ala Glu Gln Tyr Thr Val Val Asp 
    50                  55                  60                  


Arg Lys Glu Asp Ala Trp Gly Met Lys His Leu Lys Leu Glu Lys Gln 
65                  70                  75                  80  


Thr Glu Gly Val Thr Val Asp Ser Asp Asn Val Ile Ile His Leu Asp 
                85                  90                  95      


Arg Asn Gly Ala Val Thr Ser Val Thr Gly Asn Pro Val Asp Gln Val 
            100                 105                 110         


Val Lys Ile Gln Ser Val Asp Ala Ile Gly Glu Glu Gly Val Lys Lys 
        115                 120                 125             


Ile Ile Ala Ser Asp Asn Pro Glu Thr Lys Asp Leu Val Phe Leu Ala 
    130                 135                 140                 


Ile Asp Lys Arg Val Asn Asn Glu Gly Gln Leu Phe Tyr Lys Val Arg 
145                 150                 155                 160 


Val Thr Ser Ser Pro Thr Gly Asp Pro Val Ser Leu Val Tyr Lys Val 
                165                 170                 175     


Asn Ala Thr Asp Gly Thr Ile Met Glu Lys Gln Asp Leu Thr Glu His 
            180                 185                 190         


Val Gly Ser Glu Val Thr Leu Lys Asn Ser Phe Gln Val Ala Phe Asn 
        195                 200                 205             


Val Pro Val Glu Lys Ser Asn Thr Gly Ile Ala Leu His Gly Thr Asp 
    210                 215                 220                 


Asn Thr Gly Val Tyr His Ala Val Val Asp Gly Lys Asn Asn Tyr Ser 
225                 230                 235                 240 


Ile Ile Gln Ala Pro Ser Leu Val Ala Leu Asn Gln Asn Ala Val Asp 
                245                 250                 255     


Ala Tyr Thr His Gly Lys Phe Val Lys Thr Tyr Tyr Glu Asp His Phe 
            260                 265                 270         


Gln Arg His Ser Ile Asp Asp Arg Gly Met Pro Ile Leu Ser Val Val 
        275                 280                 285             


Asp Glu Gln His Pro Asp Ala Tyr Asp Asn Ala Phe Trp Asp Gly Lys 
    290                 295                 300                 


Ala Met Arg Tyr Gly Glu Thr Ser Thr Pro Thr Gly Lys Thr Tyr Ala 
305                 310                 315                 320 


Ser Ser Leu Asp Val Val Gly His Glu Met Thr His Gly Val Thr Glu 
                325                 330                 335     


His Thr Ala Gly Leu Glu Tyr Leu Gly Gln Ser Gly Ala Leu Asn Glu 
            340                 345                 350         


Ser Tyr Ser Asp Leu Met Gly Tyr Ile Ile Ser Gly Ala Ser Asn Pro 
        355                 360                 365             


Glu Ile Gly Ala Asp Thr Gln Ser Val Asp Arg Lys Thr Gly Ile Arg 
    370                 375                 380                 


Asn Leu Gln Thr Pro Ser Lys His Gly Gln Pro Glu Thr Met Ala Gln 
385                 390                 395                 400 


Tyr Asp Asp Arg Ala Arg Tyr Lys Gly Thr Pro Tyr Tyr Asp Gln Gly 
                405                 410                 415     


Gly Val His Tyr Asn Ser Gly Ile Ile Asn Arg Ile Gly Tyr Thr Ile 
            420                 425                 430         


Ile Gln Asn Leu Gly Ile Glu Lys Ala Gln Thr Ile Phe Tyr Ser Ser 
        435                 440                 445             


Leu Val Asn Tyr Leu Thr Pro Lys Ala Gln Phe Ser Asp Ala Arg Asp 
    450                 455                 460                 


Ala Met Leu Ala Ala Ala Lys Val Gln Tyr Gly Asp Glu Ala Ala Ser 
465                 470                 475                 480 


Val Val Ser Ala Ala Phe Asn Ser Ala Gly Ile Gly Ala Lys Glu Asp 
                485                 490                 495     


Ile Gln Val Asn Gln Pro Ser Glu Ser Val Leu Val Asn Glu 
            500                 505                 510 


<210>  7
<211>  1513
<212>  DNA
<213>  Clostridium perfringens

<400>  7
taaattgaga gtttgatcct ggctcaggat gaacgctggc ggcgtgctta acacatgcaa       60

gtcgagcgat gaagtttcct tcgggaaacg gattagcggc ggacgggtga gtaacacgtg      120

ggtaacctgc ctcatagagt ggaatagcct tccgaaagga agattaatac cgcataacgt      180

tgaaagatgg catcatcatt caaccaaagg agcaatccgc tatgagatgg acccgcggcg      240

cattagctag ttggtggggt aacggcctac caaggcgacg atgcgtagcc gacctgagag      300

ggtgatcggc cacattggga ctgagacacg gcccagactc ctacgggagg cagcagtggg      360

gaatattgca caatggggga aaccctgatg cagcaacgcc gcgtgagtga tgaaggtttt      420

cggatcgtaa agctctgtct ttggggaaga taatgacggt acccaaggag gaagccacgg      480

ctaactacgt gccagcagcc gcggtaatac gtaggtggcg agcgttatcc ggatttactg      540

ggcgtaaagg gagcgtaggc ggatgattaa gtgggatgtg aaatacccgg gctcaacttg      600

ggtgctgcat tccaaactgg ttatctagag tgcaggagag gagagtggaa ttcctagtgt      660

agcggtgaaa tgcgtagaga ttaggaagaa caccagtggc gaaggcgact ctctggactg      720

taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc      780

acgccgtaaa cgatgaatac taggtgtggg ggtttcaaca cctccgtgcc gccgctaacg      840

cattaagtat tccgcctggg gagtacggtc gcaagattaa aactcaaagg aattgacggg      900

gacccgcaca agtagcggag catgtggttt aattcgaagc aacgcgaaga accttaccta      960

cacttgacat cccttgcatt actcttaatc gaggaaatcc cttcggggac aaggtgacag     1020

gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc     1080

gcaacccttg tcgttagtta ctaccattaa gttgaggact ctagcgagac tgcctgggtt     1140

aaccaggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgtgt agggctacac     1200

acgtgctaca atggctggta cagagagatg caataccgcg aggtggagcc aaacttaaaa     1260

accagtctca gttcggattg taggctgaaa ctcgcctaca tgaagctgga gttactagta     1320

atcgcgaatc agaatgtcgc ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcac     1380

accatgagag ttggcaatac ccgaagtccg tgagctaacc gcaaggaggc agcggccgaa     1440

ggtagggtca gcgattgggg tgaagtcgta acaaggtagc cgtaggagaa cctgcggctg     1500

gatcacctcc ttt                                                        1513


<210>  8
<211>  1104
<212>  PRT
<213>  Clostridium perfringens

<400>  8

Met Lys Lys Asn Leu Lys Arg Gly Glu Leu Thr Lys Leu Lys Leu Val 
1               5                   10                  15      


Glu Arg Trp Ser Ala Thr Phe Thr Leu Ala Ala Phe Ile Leu Phe Asn 
            20                  25                  30          


Ser Ser Phe Lys Val Leu Ala Ala Asp Lys Lys Val Glu Asn Ser Asn 
        35                  40                  45              


Asn Gly Gln Ile Thr Arg Glu Ile Asn Ala Asp Gln Ile Ser Lys Thr 
    50                  55                  60                  


Glu Leu Asn Asn Glu Val Ala Thr Asp Asn Asn Arg Pro Leu Gly Pro 
65                  70                  75                  80  


Ser Ile Ala Pro Ser Arg Ala Arg Asn Asn Lys Ile Tyr Thr Phe Asp 
                85                  90                  95      


Glu Leu Asn Arg Met Asn Tyr Ser Asp Leu Val Glu Leu Ile Lys Thr 
            100                 105                 110         


Ile Ser Tyr Glu Asn Val Pro Asp Leu Phe Asn Phe Asn Asp Gly Ser 
        115                 120                 125             


Tyr Thr Phe Phe Ser Asn Arg Asp Arg Val Gln Ala Ile Ile Tyr Gly 
    130                 135                 140                 


Leu Glu Asp Ser Gly Arg Thr Tyr Thr Ala Asp Asp Asp Lys Gly Ile 
145                 150                 155                 160 


Pro Thr Leu Val Glu Phe Leu Arg Ala Gly Tyr Tyr Leu Gly Phe Tyr 
                165                 170                 175     


Asn Lys Gln Leu Ser Tyr Leu Asn Thr Pro Gln Leu Lys Asn Glu Cys 
            180                 185                 190         


Leu Pro Ala Met Lys Ala Ile Gln Tyr Asn Ser Asn Phe Arg Leu Gly 
        195                 200                 205             


Thr Lys Ala Gln Asp Gly Val Val Glu Ala Leu Gly Arg Leu Ile Gly 
    210                 215                 220                 


Asn Ala Ser Ala Asp Pro Glu Val Ile Asn Asn Cys Ile Tyr Val Leu 
225                 230                 235                 240 


Ser Asp Phe Lys Asp Asn Ile Asp Lys Tyr Gly Ser Asn Tyr Ser Lys 
                245                 250                 255     


Gly Asn Ala Val Phe Asn Leu Met Lys Gly Ile Asp Tyr Tyr Thr Asn 
            260                 265                 270         


Ser Val Ile Tyr Asn Thr Lys Gly Tyr Asp Ala Lys Asn Thr Glu Phe 
        275                 280                 285             


Tyr Asn Arg Ile Asp Pro Tyr Met Glu Arg Leu Glu Ser Leu Cys Thr 
    290                 295                 300                 


Ile Gly Asp Lys Leu Asn Asn Asp Asn Ala Trp Leu Val Asn Asn Ala 
305                 310                 315                 320 


Leu Tyr Tyr Thr Gly Arg Met Gly Lys Phe Arg Glu Asp Pro Ser Ile 
                325                 330                 335     


Ser Gln Arg Ala Leu Glu Arg Ala Met Lys Glu Tyr Pro Tyr Leu Ser 
            340                 345                 350         


Tyr Gln Tyr Ile Glu Ala Ala Asn Asp Leu Asp Leu Asn Phe Gly Gly 
        355                 360                 365             


Lys Asn Ser Ser Gly Asn Asp Ile Asp Phe Asn Lys Ile Lys Ala Asp 
    370                 375                 380                 


Ala Arg Glu Lys Tyr Leu Pro Lys Thr Tyr Thr Phe Asp Asp Gly Lys 
385                 390                 395                 400 


Phe Val Val Lys Ala Gly Asp Lys Val Thr Glu Glu Lys Ile Lys Arg 
                405                 410                 415     


Leu Tyr Trp Ala Ser Lys Glu Val Lys Ala Gln Phe Met Arg Val Val 
            420                 425                 430         


Gln Asn Asp Lys Ala Leu Glu Glu Gly Asn Pro Asp Asp Ile Leu Thr 
        435                 440                 445             


Val Val Ile Tyr Asn Ser Pro Glu Glu Tyr Lys Leu Asn Arg Ile Ile 
    450                 455                 460                 


Asn Gly Phe Ser Thr Asp Asn Gly Gly Ile Tyr Ile Glu Asn Ile Gly 
465                 470                 475                 480 


Thr Phe Phe Thr Tyr Glu Arg Thr Pro Glu Glu Ser Ile Tyr Thr Leu 
                485                 490                 495     


Glu Glu Leu Phe Arg His Glu Phe Thr His Tyr Leu Gln Gly Arg Tyr 
            500                 505                 510         


Val Val Pro Gly Met Trp Gly Gln Gly Glu Phe Tyr Gln Glu Gly Val 
        515                 520                 525             


Leu Thr Trp Tyr Glu Glu Gly Thr Ala Glu Phe Phe Ala Gly Ser Thr 
    530                 535                 540                 


Arg Thr Asp Gly Ile Lys Pro Arg Lys Ser Val Thr Gln Gly Leu Ala 
545                 550                 555                 560 


Tyr Asp Arg Asn Asn Arg Met Ser Leu Tyr Gly Val Leu His Ala Lys 
                565                 570                 575     


Tyr Gly Ser Trp Asp Phe Tyr Asn Tyr Gly Phe Ala Leu Ser Asn Tyr 
            580                 585                 590         


Met Tyr Asn Asn Asn Met Gly Met Phe Asn Lys Met Thr Asn Tyr Ile 
        595                 600                 605             


Lys Asn Asn Asp Val Ser Gly Tyr Lys Asp Tyr Ile Ala Ser Met Ser 
    610                 615                 620                 


Ser Asp Tyr Gly Leu Asn Asp Lys Tyr Gln Asp Tyr Met Asp Ser Leu 
625                 630                 635                 640 


Leu Asn Asn Ile Asp Asn Leu Asp Val Pro Leu Val Ser Asp Glu Tyr 
                645                 650                 655     


Val Asn Gly His Glu Ala Lys Asp Ile Asn Glu Ile Thr Asn Asp Ile 
            660                 665                 670         


Lys Glu Val Ser Asn Ile Lys Asp Leu Ser Ser Asn Val Glu Lys Ser 
        675                 680                 685             


Gln Phe Phe Thr Thr Tyr Asp Met Arg Gly Thr Tyr Val Gly Gly Arg 
    690                 695                 700                 


Ser Gln Gly Glu Glu Asn Asp Trp Lys Asp Met Asn Ser Lys Leu Asn 
705                 710                 715                 720 


Asp Ile Leu Lys Glu Leu Ser Lys Lys Ser Trp Asn Gly Tyr Lys Thr 
                725                 730                 735     


Val Thr Ala Tyr Phe Val Asn His Lys Val Asp Gly Asn Gly Asn Tyr 
            740                 745                 750         


Val Tyr Asp Val Val Phe His Gly Met Asn Thr Asp Thr Asn Thr Asp 
        755                 760                 765             


Val His Val Asn Lys Glu Pro Lys Ala Val Ile Lys Ser Asp Ser Ser 
    770                 775                 780                 


Val Ile Val Glu Glu Glu Ile Asn Phe Asp Gly Thr Glu Ser Lys Asp 
785                 790                 795                 800 


Glu Asp Gly Glu Ile Lys Ala Tyr Glu Trp Asp Phe Gly Asp Gly Glu 
                805                 810                 815     


Lys Ser Asn Glu Ala Lys Ala Thr His Lys Tyr Asn Lys Thr Gly Glu 
            820                 825                 830         


Tyr Glu Val Lys Leu Thr Val Thr Asp Asn Asn Gly Gly Ile Asn Thr 
        835                 840                 845             


Glu Ser Lys Lys Ile Lys Val Val Glu Asp Lys Pro Val Glu Val Ile 
    850                 855                 860                 


Asn Glu Ser Glu Pro Asn Asn Asp Phe Glu Lys Ala Asn Gln Ile Ala 
865                 870                 875                 880 


Lys Ser Asn Met Leu Val Lys Gly Thr Leu Ser Glu Glu Asp Tyr Ser 
                885                 890                 895     


Asp Lys Tyr Tyr Phe Asp Val Ala Lys Lys Gly Asn Val Lys Ile Thr 
            900                 905                 910         


Leu Asn Asn Leu Asn Ser Val Gly Ile Thr Trp Thr Leu Tyr Lys Glu 
        915                 920                 925             


Gly Asp Leu Asn Asn Tyr Val Leu Tyr Ala Thr Gly Asn Asp Gly Thr 
    930                 935                 940                 


Val Leu Lys Gly Glu Lys Thr Leu Glu Pro Gly Arg Tyr Tyr Leu Ser 
945                 950                 955                 960 


Val Tyr Thr Tyr Asp Asn Gln Ser Gly Thr Tyr Thr Val Asn Val Lys 
                965                 970                 975     


Gly Asn Leu Lys Asn Glu Val Lys Glu Thr Ala Lys Asp Ala Ile Lys 
            980                 985                 990         


Glu Val Glu Asn Asn Asn Asp Phe  Asp Lys Ala Met Lys  Val Asp Ser 
        995                 1000                 1005             


Asn Ser  Lys Ile Val Gly Thr  Leu Ser Asn Asp Asp  Leu Lys Asp 
    1010                 1015                 1020             


Ile Tyr  Ser Ile Asp Ile Gln  Asn Pro Ser Asp Leu  Asn Ile Val 
    1025                 1030                 1035             


Val Glu  Asn Leu Asp Asn Ile  Lys Met Asn Trp Leu  Leu Tyr Ser 
    1040                 1045                 1050             


Ala Asp  Asp Leu Ser Asn Tyr  Val Asp Tyr Ala Asn  Ala Asp Gly 
    1055                 1060                 1065             


Asn Lys  Leu Ser Asn Thr Cys  Lys Leu Asn Pro Gly  Lys Tyr Tyr 
    1070                 1075                 1080             


Leu Cys  Val Tyr Gln Phe Glu  Asn Ser Gly Thr Gly  Asn Tyr Ile 
    1085                 1090                 1095             


Val Asn  Leu Gln Asn Lys 
    1100                 


<210>  9
<211>  1021
<212>  PRT
<213>  Clostridium histolyticum

<400>  9

Met Lys Arg Lys Cys Leu Ser Lys Arg Leu Met Leu Ala Ile Thr Met 
1               5                   10                  15      


Ala Thr Ile Phe Thr Val Asn Ser Thr Leu Pro Ile Tyr Ala Ala Val 
            20                  25                  30          


Asp Lys Asn Asn Ala Thr Ala Ala Val Gln Asn Glu Ser Lys Arg Tyr 
        35                  40                  45              


Thr Val Ser Tyr Leu Lys Thr Leu Asn Tyr Tyr Asp Leu Val Asp Leu 
    50                  55                  60                  


Leu Val Lys Thr Glu Ile Glu Asn Leu Pro Asp Leu Phe Gln Tyr Ser 
65                  70                  75                  80  


Ser Asp Ala Lys Glu Phe Tyr Gly Asn Lys Thr Arg Met Ser Phe Ile 
                85                  90                  95      


Met Asp Glu Ile Gly Arg Arg Ala Pro Gln Tyr Thr Glu Ile Asp His 
            100                 105                 110         


Lys Gly Ile Pro Thr Leu Val Glu Val Val Arg Ala Gly Phe Tyr Leu 
        115                 120                 125             


Gly Phe His Asn Lys Glu Leu Asn Glu Ile Asn Lys Arg Ser Phe Lys 
    130                 135                 140                 


Glu Arg Val Ile Pro Ser Ile Leu Ala Ile Gln Lys Asn Pro Asn Phe 
145                 150                 155                 160 


Lys Leu Gly Thr Glu Val Gln Asp Lys Ile Val Ser Ala Thr Gly Leu 
                165                 170                 175     


Leu Ala Gly Asn Glu Thr Ala Pro Pro Glu Val Val Asn Asn Phe Thr 
            180                 185                 190         


Pro Ile Leu Gln Asp Cys Ile Lys Asn Ile Asp Arg Tyr Ala Leu Asp 
        195                 200                 205             


Asp Leu Lys Ser Lys Ala Leu Phe Asn Val Leu Ala Ala Pro Thr Tyr 
    210                 215                 220                 


Asp Ile Thr Glu Tyr Leu Arg Ala Thr Lys Glu Lys Pro Glu Asn Thr 
225                 230                 235                 240 


Pro Trp Tyr Gly Lys Ile Asp Gly Phe Ile Asn Glu Leu Lys Lys Leu 
                245                 250                 255     


Ala Leu Tyr Gly Lys Ile Asn Asp Asn Asn Ser Trp Ile Ile Asp Asn 
            260                 265                 270         


Gly Ile Tyr His Ile Ala Pro Leu Gly Lys Leu His Ser Asn Asn Lys 
        275                 280                 285             


Ile Gly Ile Glu Thr Leu Thr Glu Val Met Lys Val Tyr Pro Tyr Leu 
    290                 295                 300                 


Ser Met Gln His Leu Gln Ser Ala Asp Gln Ile Lys Arg His Tyr Asp 
305                 310                 315                 320 


Ser Lys Asp Ala Glu Gly Asn Lys Ile Pro Leu Asp Lys Phe Lys Lys 
                325                 330                 335     


Glu Gly Lys Glu Lys Tyr Cys Pro Lys Thr Tyr Thr Phe Asp Asp Gly 
            340                 345                 350         


Lys Val Ile Ile Lys Ala Gly Ala Arg Val Glu Glu Glu Lys Val Lys 
        355                 360                 365             


Arg Leu Tyr Trp Ala Ser Lys Glu Val Asn Ser Gln Phe Phe Arg Val 
    370                 375                 380                 


Tyr Gly Ile Asp Lys Pro Leu Glu Glu Gly Asn Pro Asp Asp Ile Leu 
385                 390                 395                 400 


Thr Met Val Ile Tyr Asn Ser Pro Glu Glu Tyr Lys Leu Asn Ser Val 
                405                 410                 415     


Leu Tyr Gly Tyr Asp Thr Asn Asn Gly Gly Met Tyr Ile Glu Pro Glu 
            420                 425                 430         


Gly Thr Phe Phe Thr Tyr Glu Arg Glu Ala Gln Glu Ser Thr Tyr Thr 
        435                 440                 445             


Leu Glu Glu Leu Phe Arg His Glu Tyr Thr His Tyr Leu Gln Gly Arg 
    450                 455                 460                 


Tyr Ala Val Pro Gly Gln Trp Gly Arg Thr Lys Leu Tyr Asp Asn Asp 
465                 470                 475                 480 


Arg Leu Thr Trp Tyr Glu Glu Gly Gly Ala Glu Leu Phe Ala Gly Ser 
                485                 490                 495     


Thr Arg Thr Ser Gly Ile Leu Pro Arg Lys Ser Ile Val Ser Asn Ile 
            500                 505                 510         


His Asn Thr Thr Arg Asn Asn Arg Tyr Lys Leu Ser Asp Thr Val His 
        515                 520                 525             


Ser Lys Tyr Gly Ala Ser Phe Glu Phe Tyr Asn Tyr Ala Cys Met Phe 
    530                 535                 540                 


Met Asp Tyr Met Tyr Asn Lys Asp Met Gly Ile Leu Asn Lys Leu Asn 
545                 550                 555                 560 


Asp Leu Ala Lys Asn Asn Asp Val Asp Gly Tyr Asp Asn Tyr Ile Arg 
                565                 570                 575     


Asp Leu Ser Ser Asn Tyr Ala Leu Asn Asp Lys Tyr Gln Asp His Met 
            580                 585                 590         


Gln Glu Arg Ile Asp Asn Tyr Glu Asn Leu Thr Val Pro Phe Val Ala 
        595                 600                 605             


Asp Asp Tyr Leu Val Arg His Ala Tyr Lys Asn Pro Asn Glu Ile Tyr 
    610                 615                 620                 


Ser Glu Ile Ser Glu Val Ala Lys Leu Lys Asp Ala Lys Ser Glu Val 
625                 630                 635                 640 


Lys Lys Ser Gln Tyr Phe Ser Thr Phe Thr Leu Arg Gly Ser Tyr Thr 
                645                 650                 655     


Gly Gly Ala Ser Lys Gly Lys Leu Glu Asp Gln Lys Ala Met Asn Lys 
            660                 665                 670         


Phe Ile Asp Asp Ser Leu Lys Lys Leu Asp Thr Tyr Ser Trp Ser Gly 
        675                 680                 685             


Tyr Lys Thr Leu Thr Ala Tyr Phe Thr Asn Tyr Lys Val Asp Ser Ser 
    690                 695                 700                 


Asn Arg Val Thr Tyr Asp Val Val Phe His Gly Tyr Leu Pro Asn Glu 
705                 710                 715                 720 


Gly Asp Ser Lys Asn Ser Leu Pro Tyr Gly Lys Ile Asn Gly Thr Tyr 
                725                 730                 735     


Lys Gly Thr Glu Lys Glu Lys Ile Lys Phe Ser Ser Glu Gly Ser Phe 
            740                 745                 750         


Asp Pro Asp Gly Lys Ile Val Ser Tyr Glu Trp Asp Phe Gly Asp Gly 
        755                 760                 765             


Asn Lys Ser Asn Glu Glu Asn Pro Glu His Ser Tyr Asp Lys Val Gly 
    770                 775                 780                 


Thr Tyr Thr Val Lys Leu Lys Val Thr Asp Asp Lys Gly Glu Ser Ser 
785                 790                 795                 800 


Val Ser Thr Thr Thr Ala Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu 
                805                 810                 815     


Pro Val Ile Tyr Met His Val Pro Lys Ser Gly Ala Leu Asn Gln Lys 
            820                 825                 830         


Val Val Phe Tyr Gly Lys Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala 
        835                 840                 845             


Gly Tyr Gln Trp Asp Phe Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln 
    850                 855                 860                 


Asn Pro Ser His Val Tyr Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu 
865                 870                 875                 880 


Arg Val Met Asp Ser Ser Gly Gln Met Ser Glu Lys Thr Met Lys Ile 
                885                 890                 895     


Lys Ile Thr Asp Pro Val Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn 
            900                 905                 910         


Asn Ser Lys Glu Thr Ala Ser Gly Pro Ile Val Pro Gly Ile Pro Val 
        915                 920                 925             


Ser Gly Thr Ile Glu Asn Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp 
    930                 935                 940                 


Val Ile Thr Pro Gly Glu Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr 
945                 950                 955                 960 


Gly Gly Ala Thr Trp Val Val Tyr Asp Glu Asn Asn Asn Ala Val Ser 
                965                 970                 975     


Tyr Ala Thr Asp Asp Gly Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp 
            980                 985                 990         


Lys Pro Gly Arg Tyr Tyr Ile His  Leu Tyr Met Phe Asn  Gly Ser Tyr 
        995                 1000                 1005             


Met Pro  Tyr Arg Ile Asn Ile  Glu Gly Ser Val Gly  Arg 
    1010                 1015                 1020     


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRET-based enzyme substrate


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa is 7-methoxy-coumarin-4-yl-acetic acid-2,4-
       dinitrophenyl-lysine

<220>
<221>  SITE
<222>  (6)..(6)
<223>  Xaa is N beta-2,4-dinitrophenyl-L-di-aminopropionic acid

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  AMIDATION

<400>  10

Xaa Pro Leu Gly Leu Xaa Ala Arg 
1               5               


