                               SEQUENCE LISTING

<110> METAGENOMI IP TECHNOLOGIES, LLC
 
<120> BASE EDITING ENZYMES

<130> 55921-715.601

<140>
<141>

<150> 63/222,351
<151> 2021-07-15

<150> 63/077,057
<151> 2020-09-11

<160> 593   

<170> PatentIn version 3.5

<210> 1
<211> 186
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-2 deaminase

<400> 1
Met Ala Arg Val Leu Gly Leu Asp Pro Gly Phe Ala Thr Cys Gly Tyr 
1               5                   10                  15      


Ala Leu Val Asp Leu Thr Arg Lys Asp Gly Ala Pro Phe Val Asp Val 
            20                  25                  30          


Arg Ser Leu Gly Thr Leu Arg Thr Ala Lys Ala Asp Lys Lys Arg Lys 
        35                  40                  45              


Val Leu Ala Ala Asp Asp Asn Leu Arg Arg Ala Gln Glu Leu Ile Arg 
    50                  55                  60                  


Ala Leu Tyr Pro Leu Ala Ser Asn Ala Gln Ala Ile Val Ala Glu Ser 
65                  70                  75                  80  


Met Ser Phe Pro Arg Asn Ser Ala Ala Ala Ala Lys Met Ala Met Cys 
                85                  90                  95      


Trp Gly Ile Val Ala Thr Leu Ala Glu Met Asn Asp Leu Pro Ile Val 
            100                 105                 110         


Gln Ala Thr Pro Lys Glu Ile Lys Ile Ala Val Ala Gly Val Gly Thr 
        115                 120                 125             


Ala Thr Lys Glu Asp Val Ala Ala Ala Val Cys Ala Lys Cys Gly Arg 
    130                 135                 140                 


Gln Phe Ser Glu Glu Leu Leu Ala Gln Gly Leu Pro Lys Gly Met His 
145                 150                 155                 160 


Glu His Ala Tyr Asp Ala Val Ala Ser Val Leu Ala Cys Leu Asn Ser 
                165                 170                 175     


Asp Val Leu Arg Ala Ile Arg Arg Ala Gly 
            180                 185     


<210> 2
<211> 164
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-3 deaminase

<400> 2
Met Asp Arg Leu Ser Leu His Gln Tyr Ala Ile Ala Leu Ala Trp Val 
1               5                   10                  15      


Ala Ala Thr Arg Ser Glu Asp Pro Tyr Thr Arg Val Gly Cys Val Ala 
            20                  25                  30          


Leu Ser Glu Gln Gly Asn Val Leu Ala Thr Ala Tyr Asn Gly Leu Lys 
        35                  40                  45              


Pro Lys Ala Thr Phe Gly Pro Glu Phe Trp Asn Asn Arg Glu Ser Arg 
    50                  55                  60                  


Leu Pro Phe Met Val His Ala Glu Met Asn Leu Val Ser Leu Thr Lys 
65                  70                  75                  80  


Arg Asn Glu Val His Thr Val Ala Ile Asn Ile Leu Pro Cys Pro Thr 
                85                  90                  95      


Cys Ala Ala Phe Leu Ala Thr His Gly Val Gln Gln Val Val Tyr Ser 
            100                 105                 110         


Glu Val Tyr His Arg Asp Lys Gly Thr Val Gly Leu Thr Val Leu Arg 
        115                 120                 125             


Glu Tyr Gly Val Glu Ile His His Leu Pro Tyr Tyr Arg Val Arg Pro 
    130                 135                 140                 


Val Phe Leu Asp Met Asp Glu Ala His Arg Lys Val Arg Ser Glu Gln 
145                 150                 155                 160 


Arg Thr Ala Ala 
                


<210> 3
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-4 deaminase

<400> 3
Met Arg Glu Tyr Ile Gln Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


His Val Val Ser Ser Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Thr Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Gly Glu Glu 
    130                 135 


<210> 4
<211> 160
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-5 deaminase

<400> 4
Met Ser Asn Thr Gln Ser Leu His Thr Thr Phe Met His Ile Leu Phe 
1               5                   10                  15      


Glu Phe Ser Lys Leu Ser His Cys Val Ser Lys Gln Val Ala Ala Met 
            20                  25                  30          


Ala Val Lys Asp Gly Arg Ile Ile Ser Thr Gly Ile Asn Gly Thr Ala 
        35                  40                  45              


Pro Gly Tyr Thr Asn Cys Asp Asp Ile Phe Asn Lys Ser Asp Phe Asn 
    50                  55                  60                  


Arg Glu Leu His His Gln Phe Ser Asn Thr Tyr Glu Ile His Ala Glu 
65                  70                  75                  80  


Met Asn Met Ile Leu Tyr Ala Ala Lys Asn Gly Ile Ser Leu Asp Gly 
                85                  90                  95      


Ala Thr Leu Tyr Cys Ser Met Leu Pro Cys Trp Asn Cys Ile Lys His 
            100                 105                 110         


Ile Ser Val Thr Gly Ile Lys His Ile Ile Tyr Ala Lys Arg Tyr Asp 
        115                 120                 125             


Met Leu Ser Leu Glu Asp Glu Lys Asn Ile Tyr Tyr Tyr Cys Lys Lys 
    130                 135                 140                 


Leu Gly Ile Thr Ile Gln Gln Phe Asp Pro Asp Thr Leu Gly Ser Phe 
145                 150                 155                 160 


<210> 5
<211> 124
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-6 deaminase

<400> 5
Met Thr Ser Gly Ile Leu Lys Leu Ala Leu Glu Glu Ala Glu Ser Ser 
1               5                   10                  15      


Thr Tyr Arg Phe Arg Val Gly Ala Val Val Phe Lys Gly Ser Arg Ile 
            20                  25                  30          


Leu Ala Ser Gly His Asn Gly Ile Arg Ser Ser Arg Ile His Pro Leu 
        35                  40                  45              


His Lys Asn Tyr Asn Asn Ala Leu His Ala Glu Gln Asp Ala Ile Leu 
    50                  55                  60                  


Asn Val Lys Asp Trp Ser Thr Leu Lys Gly Cys Ser Ile Leu Val Val 
65                  70                  75                  80  


Lys Val Ser Lys Thr Lys Gly Thr Ile Ser Met Ala Lys Pro Cys Pro 
                85                  90                  95      


Met Cys Gln Gln Leu Leu Arg His Val Gly Ile Lys Thr Met Tyr Phe 
            100                 105                 110         


Ser Asn Glu Asn Gly Glu Ile Ile Ser Glu Lys Leu 
        115                 120                 


<210> 6
<211> 132
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-7 deaminase

<400> 6
Met Thr Lys Arg Glu Ile Ala Tyr Phe Asn Ala Ala Lys Ala Val Ser 
1               5                   10                  15      


Ser Leu Ser Asp His Lys Tyr Lys Met Gly Cys Val Ile Val Asn Lys 
            20                  25                  30          


His Arg Ile Ile Ser Ser Gly Phe Asn Ser Glu Thr Lys Cys His Arg 
        35                  40                  45              


Ile Gln Ala Glu Leu Asp Met Asn Glu Tyr Lys Met His Ser Ser Gly 
    50                  55                  60                  


Lys Val His Ala Glu Thr Ser Ala Leu Leu Ser Leu Arg His Ile Asp 
65                  70                  75                  80  


Leu Ser Lys Ala Ser Ile Phe Ile Tyr Arg Glu Leu Lys Asp Gly Thr 
                85                  90                  95      


Lys Ala Leu Ala Arg Pro Cys Pro Cys Cys Gln Lys Leu Ile Arg Lys 
            100                 105                 110         


Ala Gly Ile Lys His Val Tyr Tyr Thr Gly Asn Asn Ser Leu Ile Tyr 
        115                 120                 125             


Glu Glu Phe Ile 
    130         


<210> 7
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-8 deaminase

<400> 7
Ala Ala Gly Asp Phe Val Gln Val Arg Glu Tyr Ile Lys Ala Ala Arg 
1               5                   10                  15      


Asp Glu Ala Ala Lys Ser Arg Cys Asp Arg Ala His Val Gly Cys Val 
            20                  25                  30          


Ile Val Asp Arg Val Thr Gly Arg Val Val Ser Arg Ala Phe Asn Glu 
        35                  40                  45              


Thr Pro His Gly Leu Glu Pro Cys Asp Thr Gly Gly His Arg Met Ile 
    50                  55                  60                  


Asp Gly His Cys Val Asn Thr Val His Ala Glu Arg Asn Ala Ile Arg 
65                  70                  75                  80  


Lys Met Lys Glu His Glu Ser Glu Tyr Thr Leu Tyr Val Thr His Tyr 
                85                  90                  95      


Pro Cys Gln Gly Cys Ala His Leu Ile Ala Ser Cys Pro Glu Ile Val 
            100                 105                 110         


Glu Val Val Tyr Leu Gly Asp Tyr Arg Asn Ser Lys Glu Ala Thr Ala 
        115                 120                 125             


Leu Leu Met Gly Leu Ser Lys Gly Val His Arg Gly Glu Glu 
    130                 135                 140         


<210> 8
<211> 82
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-9 deaminase

<400> 8
His Arg Ile Val Asp Asn His Cys Val Asn Thr Val His Ala Glu Arg 
1               5                   10                  15      


Asn Ala Ile Arg Lys Met Lys Glu His Gly Ser Glu Tyr Thr Leu Tyr 
            20                  25                  30          


Val Thr His Tyr Pro Cys Gln Gly Cys Ala His Leu Ile Ser Ser Cys 
        35                  40                  45              


Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp Tyr Arg Asn Ser Ser 
    50                  55                  60                  


Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys Gly Val His Arg Gly 
65                  70                  75                  80  


Glu Glu 
        


<210> 9
<211> 115
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-10 deaminase

<400> 9
His Val Gly Cys Val Ile Val Asp His Ala Thr Gly Gln Val Val Ser 
1               5                   10                  15      


Ser Ala Phe Asn Glu Thr Pro Pro Gly Leu Glu Pro Cys Asp Thr Gly 
            20                  25                  30          


Gly His Arg Ile Val Asp Asp His Cys Val Asn Thr Val His Ala Glu 
        35                  40                  45              


Arg Gly Ala Ile Arg Lys Met Ala Glu His Gly Ser Glu Tyr Thr Leu 
    50                  55                  60                  


Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His Leu Ile Ser Ser 
65                  70                  75                  80  


Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp Tyr Asn Asn Ser 
                85                  90                  95      


Ser Glu Ala Thr Asp Leu Leu Met Ser Leu Ser Lys Gly Val His Arg 
            100                 105                 110         


Ala Glu Glu 
        115 


<210> 10
<211> 186
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-11 deaminase

<400> 10
Met Ala Arg Val Leu Gly Leu Asp Pro Gly Phe Ala Thr Cys Gly Tyr 
1               5                   10                  15      


Ala Leu Val Asp Leu Thr Arg Lys Asp Gly Ala Pro Phe Val Asp Val 
            20                  25                  30          


Arg Ser Leu Gly Thr Leu Arg Thr Ala Lys Ala Asp Lys Lys Arg Lys 
        35                  40                  45              


Val Leu Ala Ala Asp Asp Asn Leu Arg Arg Ala Gln Glu Leu Ile Arg 
    50                  55                  60                  


Ala Leu Tyr Pro Leu Ala Ser Asn Ala Gln Ala Ile Val Ala Glu Ser 
65                  70                  75                  80  


Met Ser Phe Pro Arg Asn Ser Ala Ala Ala Ala Lys Met Ala Met Cys 
                85                  90                  95      


Trp Gly Ile Val Ala Thr Leu Ala Glu Met Asn Asp Leu Pro Ile Val 
            100                 105                 110         


Gln Ala Thr Pro Lys Glu Ile Lys Ile Ala Val Ala Gly Val Gly Thr 
        115                 120                 125             


Ala Thr Lys Glu Asp Val Ala Ala Ala Val Cys Ala Lys Cys Gly Arg 
    130                 135                 140                 


Gln Phe Ser Glu Glu Leu Leu Ala Gln Gly Leu Pro Lys Gly Met His 
145                 150                 155                 160 


Glu His Ala Tyr Asp Ala Val Ala Ser Val Leu Ala Cys Leu Asn Ser 
                165                 170                 175     


Asp Val Leu Arg Ala Ile Arg Arg Ala Gly 
            180                 185     


<210> 11
<211> 232
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-12 deaminase

<400> 11
Met Asp Asn Leu Pro Lys Tyr Glu Tyr Leu Asn Val Leu Asp Pro Glu 
1               5                   10                  15      


Arg Thr Gly Ile Ser Glu Thr Trp Leu Ser Tyr Phe Met Asn Ile Ala 
            20                  25                  30          


Arg Val Ile Ala Thr Lys Ser Lys Asp Pro Ser Thr Lys Val Gly Ala 
        35                  40                  45              


Val Ala Val Asp Thr Asn Thr Lys Arg Ile Leu Ala Thr Gly Tyr Asn 
    50                  55                  60                  


Gly Phe Pro Ala Gly Val Lys Glu Arg Pro Glu Arg Trp Glu Arg Pro 
65                  70                  75                  80  


Val Lys Tyr Ser Tyr Ile Val His Ser Glu Ala Asn Ile Val Ala Ser 
                85                  90                  95      


Ala Ala Arg Phe Gly Ile Asn Leu Thr Gly Ser Thr Leu Phe Val Thr 
            100                 105                 110         


Leu His Pro Cys Val Asp Cys Ala Lys Ile Val Ala Ser Ala Gly Phe 
        115                 120                 125             


Lys Asn Val Ile Tyr Ile Asp Thr Glu Ile Lys Ala Gln Val Gly Arg 
    130                 135                 140                 


Glu Trp Ile Thr Leu Leu Gln His Ser Lys Asn Ile Phe Glu Glu Ser 
145                 150                 155                 160 


Arg Ile Gly Leu Tyr Ala Cys Arg Glu Val Pro Ala Lys Lys Lys Glu 
                165                 170                 175     


Glu Pro Lys Leu Tyr Lys Thr Tyr Lys Gly Thr Val Glu Thr Thr Thr 
            180                 185                 190         


Glu Leu Asn Asn Lys Arg Asp Lys Gln Leu Gly Asp Leu Val Phe Val 
        195                 200                 205             


Lys Ser Arg Asn Thr Leu Tyr Glu Trp Asn Gly Val Ile Trp Thr Val 
    210                 215                 220                 


Ile Gly Glu Tyr Gln His Leu Leu 
225                 230         


<210> 12
<211> 113
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-13 deaminase

<400> 12
Met Asp Asn Leu Pro Lys Tyr Glu Tyr Leu Asn Val Leu Asp Pro Glu 
1               5                   10                  15      


Arg Thr Arg Ile Ser Glu Thr Trp Leu Ser Tyr Phe Met Asn Ile Ala 
            20                  25                  30          


Arg Val Ile Ala Thr Lys Ser Lys Asp Pro Ser Thr Lys Val Gly Ala 
        35                  40                  45              


Val Ala Val Asp Thr Asn Thr Lys Arg Ile Leu Ala Thr Gly Tyr Asn 
    50                  55                  60                  


Gly Phe Pro Ala Gly Val Lys Glu Arg Pro Glu Arg Trp Glu Arg Pro 
65                  70                  75                  80  


Val Lys Tyr Ser Tyr Ile Val His Ser Glu Ala Asn Ile Val Ala Ser 
                85                  90                  95      


Ala Ala Arg Phe Gly Ile Asn Leu Thr Gly Ser Thr Leu Phe Val Thr 
            100                 105                 110         


Leu 
    


<210> 13
<211> 232
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-14 deaminase

<400> 13
Met Asp Asn Leu Pro Lys Tyr Glu Tyr Leu Asn Val Leu Asp Pro Glu 
1               5                   10                  15      


Arg Thr Arg Ile Ser Glu Thr Trp Leu Ser Tyr Phe Met Asn Ile Ala 
            20                  25                  30          


Arg Val Ile Ala Thr Lys Ser Lys Asp Pro Ser Thr Lys Val Gly Ala 
        35                  40                  45              


Val Ala Val Asp Thr Asn Thr Lys Arg Ile Leu Ala Thr Gly Tyr Asn 
    50                  55                  60                  


Gly Phe Pro Ala Gly Val Lys Glu Arg Pro Glu Arg Trp Glu Arg Pro 
65                  70                  75                  80  


Val Lys Tyr Ser Tyr Ile Val His Ser Glu Ala Asn Ile Val Ala Ser 
                85                  90                  95      


Ala Ala Arg Phe Gly Ile Asn Leu Thr Gly Ser Thr Leu Phe Val Thr 
            100                 105                 110         


Leu His Pro Cys Val Asp Cys Ala Lys Ile Val Ala Ser Ala Gly Phe 
        115                 120                 125             


Lys Asn Val Ile Tyr Ile Asp Thr Glu Ile Lys Pro Gln Val Gly Arg 
    130                 135                 140                 


Glu Trp Ile Thr Leu Leu Gln His Ser Lys Asn Ile Phe Glu Glu Ser 
145                 150                 155                 160 


Arg Ile Gly Leu Tyr Ala Cys Arg Glu Val Pro Ala Lys Lys Lys Glu 
                165                 170                 175     


Glu Pro Lys Leu Tyr Lys Thr Phe Lys Gly Thr Val Glu Thr Thr Val 
            180                 185                 190         


Glu Leu Asn Asn Lys Arg Asp Lys Gln Leu Gly Asp Leu Val Phe Val 
        195                 200                 205             


Lys Ser Arg Asn Thr Leu Tyr Glu Trp Asn Gly Val Ile Trp Thr Val 
    210                 215                 220                 


Ile Gly Glu Tyr Gln His Leu Leu 
225                 230         


<210> 14
<211> 164
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-15 deaminase

<400> 14
Met Asp Arg Leu Ser Leu His Gln Tyr Ala Ile Ala Leu Ala Trp Val 
1               5                   10                  15      


Ala Ala Thr Arg Ser Glu Asp Pro Tyr Thr Arg Val Gly Cys Val Ala 
            20                  25                  30          


Leu Ser Glu Gln Gly Asn Val Leu Ala Thr Ala Tyr Asn Gly Leu Lys 
        35                  40                  45              


Pro Lys Ala Thr Phe Gly Pro Glu Phe Trp Asn Asn Arg Glu Ser Arg 
    50                  55                  60                  


Leu Pro Phe Met Val His Ala Glu Met Asn Leu Val Ser Leu Thr Lys 
65                  70                  75                  80  


Arg Asn Glu Val His Thr Val Ala Ile Asn Ile Leu Pro Cys Pro Thr 
                85                  90                  95      


Cys Ala Ala Phe Leu Ala Thr His Gly Val Gln Gln Val Val Tyr Ser 
            100                 105                 110         


Glu Val Tyr His Arg Asp Lys Gly Thr Val Gly Leu Thr Val Leu Arg 
        115                 120                 125             


Glu Tyr Gly Val Glu Ile His His Leu Pro Tyr Tyr Arg Val Arg Pro 
    130                 135                 140                 


Val Phe Leu Asp Met Asp Glu Ala His Arg Lys Val Arg Ser Glu Gln 
145                 150                 155                 160 


Arg Thr Ala Ala 
                


<210> 15
<211> 50
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-18 deaminase

<400> 15
Met Lys Gln Tyr Ile Gln Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp 
    50  


<210> 16
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-19 deaminase

<400> 16
Met Lys Gln Tyr Ile Gln Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 17
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-20 deaminase

<400> 17
Met Lys Gln Tyr Ile Gln Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Gln Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Ile Glu Val Val Tyr Ile Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 18
<211> 204
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-21 deaminase

<400> 18
Met Asn Ile Ala Arg Val Ile Ala Thr Lys Ser Lys Asp Pro Ser Thr 
1               5                   10                  15      


Lys Val Gly Ala Val Ala Val Asp Thr Asn Thr Lys Arg Ile Leu Ala 
            20                  25                  30          


Thr Gly Tyr Asn Gly Phe Pro Ala Gly Val Lys Glu Arg Pro Glu Arg 
        35                  40                  45              


Trp Glu Arg Pro Val Lys Tyr Ser Tyr Ile Val His Ser Glu Ala Asn 
    50                  55                  60                  


Ile Val Ala Ser Ala Ala Arg Phe Gly Ile Asn Leu Thr Gly Ser Thr 
65                  70                  75                  80  


Leu Phe Val Thr Leu His Pro Cys Val Asp Cys Ala Lys Ile Val Ala 
                85                  90                  95      


Ser Ala Gly Phe Lys Asn Val Ile Tyr Ile Asp Thr Glu Ile Lys Pro 
            100                 105                 110         


Gln Val Gly Arg Glu Trp Ile Thr Leu Leu Gln His Ser Lys Asn Ile 
        115                 120                 125             


Phe Glu Glu Ser Arg Ile Gly Leu Tyr Ala Cys Arg Glu Val Pro Ala 
    130                 135                 140                 


Lys Lys Lys Glu Glu Pro Lys Leu Tyr Lys Thr Phe Lys Gly Thr Val 
145                 150                 155                 160 


Glu Thr Thr Val Glu Leu Asn Asn Lys Arg Asp Lys Gln Leu Gly Asp 
                165                 170                 175     


Leu Ile Phe Val Arg Ser Arg Asn Thr Leu Tyr Glu Trp Asn Gly Val 
            180                 185                 190         


Ile Trp Thr Val Ile Gly Glu Tyr Gln His Leu Leu 
        195                 200                 


<210> 19
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-22 deaminase

<400> 19
Met Arg Glu Phe Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Gln Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Asp Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Asn Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Ala Glu Glu Asp Cys Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 20
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-23 deaminase

<400> 20
Met Arg Glu Phe Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Gln Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Asn Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Ala Glu Glu Asp Cys Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 21
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-24 deaminase

<400> 21
Met Arg Glu Phe Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Gln Thr His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Asn Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Ala Glu Glu Asp Cys Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 22
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-25 deaminase

<400> 22
Met Arg Glu Phe Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Thr Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Asn Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Ala Glu Glu Asp Cys Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 23
<211> 136
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-26 deaminase

<400> 23
Met Arg Glu Leu Gly Ile Lys Ala Ala Arg Asp Glu Ala Lys Lys Ser 
1               5                   10                  15      


Arg Cys Asp Arg Ala His Val Gly Cys Val Ile Ile Asp Arg Ala Ser 
            20                  25                  30          


Gly Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro Pro Thr Leu Glu 
        35                  40                  45              


Pro Cys Asp Thr Gly Gly His Arg Met Val Asp Gly His Cys Val Asn 
    50                  55                  60                  


Thr Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Ala Glu His Gly 
65                  70                  75                  80  


Ser Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala 
                85                  90                  95      


His Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly 
            100                 105                 110         


Asp Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Met Ser Leu Ser 
        115                 120                 125             


Lys Gly Val His Arg Val Lys Gly 
    130                 135     


<210> 24
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-27 deaminase

<400> 24
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp His Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Thr Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Glu Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 25
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-28 deaminase

<400> 25
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Glu 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro Asn Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asp His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Arg Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 26
<211> 36
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-29 deaminase

<400> 26
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser 
        35      


<210> 27
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-30 deaminase

<400> 27
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Gly Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 28
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-31 deaminase

<400> 28
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 29
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-32 deaminase

<400> 29
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Thr Asp His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 30
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-33 deaminase

<400> 30
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Val Asp Asp His Cys Val Asn Thr 
    50                  55                  60                  


Ile His Ala Glu Arg Asn Ala Ile Arg Gln Met Lys Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Phe Ile Ser Thr Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 31
<211> 40
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-34 deaminase

<400> 31
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Ser Ala Phe Asn 
        35                  40  


<210> 32
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-35 deaminase

<400> 32
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Ser Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Gln Met Thr Asp His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 33
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-36 deaminase

<400> 33
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Pro Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Asp Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Glu Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Ile His Cys Ala Glu Glu Asp Arg Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 34
<211> 142
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-37 deaminase

<400> 34
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Pro Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Asp Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Glu Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Cys Ala Glu Glu Asp Arg Ser Ser Ser Pro Ala 
    130                 135                 140         


<210> 35
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-38 deaminase

<400> 35
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Lys Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Ser Gly 
            20                  25                  30          


Gln Val Val Ser Ser Ala Phe Asn Glu Thr Pro Pro Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Val Asp Asp His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Leu Lys Met Ala Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Thr Leu Leu Met Ser Leu Ser Lys 
        115                 120                 125             


Gly Val His His Val Glu Gly 
    130                 135 


<210> 36
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-39 deaminase

<400> 36
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Lys Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Lys Gly Phe Asn Glu Thr Pro Pro Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His His Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Ser Ala Ile Arg Lys Met Thr Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Leu Ile Thr Ser Cys Pro Glu Ile Met Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Glu Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Val Glu Gly 
    130                 135 


<210> 37
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-40 deaminase

<400> 37
Met Arg Glu Tyr Ile Lys Ala Ala Arg Asp Glu Ala Lys Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asp His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Arg Met Val Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 38
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-41 deaminase

<400> 38
Met Arg Glu Tyr Ile Gln Ala Ala Arg Asp Glu Ala Lys Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Ser Gly 
            20                  25                  30          


Gln Val Val Ser Ser Ala Phe Asn Glu Thr Pro Pro Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Val Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Ala Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Met Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Thr Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Val Glu Glu 
    130                 135 


<210> 39
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-42 deaminase

<400> 39
Met Arg Glu Tyr Ile Gln Ala Ala Arg Asp Glu Ala Lys Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Arg Val Val Ser Ser Ala Phe Asn Glu Thr Pro Pro Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Val Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Gly Ala Ile Arg Lys Met Ala Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Arg Gly Cys Gly His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His His Val Glu Glu 
    130                 135 


<210> 40
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-43 deaminase

<400> 40
Met Arg Glu Tyr Ile Gln Ala Ala Arg Asp Glu Ala Ser Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Ile His Ala Glu Arg Asn Ala Ile Arg Lys Met Pro Thr His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Ser Glu Ala Thr Ala Leu Leu Ser Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 41
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-44 deaminase

<400> 41
Met Arg Glu Tyr Ile Arg Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Gly 
            20                  25                  30          


Gln Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Glu Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Ile Val Asp Asn His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Thr Glu His Gly Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ser Ser Cys Pro Glu Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Asn Asn Ser Ser Glu Ala Thr Ala Leu Leu Glu Gly Leu Ser Lys 
        115                 120                 125             


Gly Val His Arg Gly Glu Glu 
    130                 135 


<210> 42
<211> 135
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-45 deaminase

<400> 42
Met Arg Glu Tyr Ile Arg Ala Ala Arg Asp Glu Ala Ala Lys Ser Arg 
1               5                   10                  15      


Cys Asp Arg Ala His Val Gly Cys Val Ile Val Asp Arg Ala Thr Ser 
            20                  25                  30          


Arg Val Val Ser Arg Ala Phe Asn Glu Thr Pro His Gly Leu Asp Pro 
        35                  40                  45              


Cys Asp Thr Gly Gly His Arg Met Ile Asp Gly His Cys Val Asn Thr 
    50                  55                  60                  


Val His Ala Glu Arg Asn Ala Ile Arg Lys Met Pro Ser His Asp Ser 
65                  70                  75                  80  


Glu Tyr Thr Leu Tyr Val Thr His Tyr Pro Cys Gln Gly Cys Ala His 
                85                  90                  95      


Leu Ile Ala Ser Cys Pro Asp Ile Val Glu Val Val Tyr Leu Gly Asp 
            100                 105                 110         


Tyr Arg Asn Ser Lys Glu Ala Thr Ala Leu Leu Met Gly Leu Ser Lys 
        115                 120                 125             


Gly Ile His His Ala Glu Glu 
    130                 135 


<210> 43
<211> 151
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-46 deaminase

<400> 43
Met Arg Val Arg Pro Asp Trp Asp Thr Tyr Phe Met Arg Ile Ala Lys 
1               5                   10                  15      


Val Val Ala Gln Arg Ser His Asp Glu Asp Thr Gln Val Gly Cys Val 
            20                  25                  30          


Ile Val Ser Pro Asp Lys Arg Ile Leu Ser Thr Gly Tyr Asn Gly Phe 
        35                  40                  45              


Pro Pro Gly Phe Pro Asp Gly Lys Leu Pro Arg Thr Arg Pro Gly Lys 
    50                  55                  60                  


Tyr Pro Tyr Ile Val His Ser Glu Ile Asn Ala Ile Val Ser Ala Leu 
65                  70                  75                  80  


Asn Arg Asp Leu Arg Gly Ala Thr Leu Tyr Cys Arg Phe Thr Pro Cys 
                85                  90                  95      


Lys Asp Cys Val Lys Ala Ile Ile Thr Ala Gly Ile Thr Arg Ile Val 
            100                 105                 110         


Tyr Glu Glu Thr Tyr Ala Arg Asp Asn Thr Asp Ala Ala Val Val Phe 
        115                 120                 125             


Glu Met Leu Glu Met Gly Gly Val Glu Ile Val Lys His Leu Glu His 
    130                 135                 140                 


Glu His Glu Ala Asp Ser Asp 
145                 150     


<210> 44
<211> 159
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-47 deaminase

<400> 44
Met Ser Asn Thr Gln Ser Leu His Thr Thr Phe Met His Ile Leu Phe 
1               5                   10                  15      


Glu Phe Ser Lys Leu Ser His Cys Val Ser Lys Gln Val Ala Ala Met 
            20                  25                  30          


Ala Val Lys Asp Gly Arg Ile Ile Ser Thr Gly Ile Asn Gly Thr Ala 
        35                  40                  45              


Pro Gly Tyr Thr Asn Cys Asp Asp Ile Phe Asn Lys Ser Asp Phe Asp 
    50                  55                  60                  


Arg Glu Leu His His Gln Phe Ser Asn Thr Tyr Glu Ile His Ala Glu 
65                  70                  75                  80  


Met Asn Met Ile Leu Tyr Ala Ala Lys Asn Gly Ile Ser Leu Asp Gly 
                85                  90                  95      


Ala Thr Leu Tyr Cys Ser Met Leu Pro Cys Trp Asn Cys Ile Lys His 
            100                 105                 110         


Ile Ser Val Thr Gly Ile Lys His Ile Ile Tyr Ala Lys Arg Tyr Asp 
        115                 120                 125             


Met Leu Ser Leu Glu Asp Glu Lys Asn Ile Tyr Tyr Tyr Cys Lys Lys 
    130                 135                 140                 


Leu Gly Ile Thr Ile Gln Gln Phe Asp Pro Asp Thr Leu Gly Ser 
145                 150                 155                 


<210> 45
<211> 160
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-48 deaminase

<400> 45
Met Ser Asn Thr Gln Ser Leu His Thr Thr Phe Met His Ile Leu Phe 
1               5                   10                  15      


Glu Phe Ser Lys Leu Ser His Cys Val Ser Lys Gln Val Ala Ala Met 
            20                  25                  30          


Ala Val Lys Asp Gly Arg Ile Ile Ser Thr Gly Ile Asn Gly Thr Ala 
        35                  40                  45              


Pro Gly Tyr Thr Asn Cys Asp Asp Ile Phe Asn Lys Ser Asp Phe Asp 
    50                  55                  60                  


Arg Glu Leu His His Gln Phe Ser Asn Thr Tyr Glu Ile His Ala Glu 
65                  70                  75                  80  


Met Asn Met Ile Leu Tyr Ala Ala Lys Asn Gly Ile Ser Leu Asp Gly 
                85                  90                  95      


Ala Thr Leu Tyr Cys Ser Met Leu Pro Cys Trp Asn Cys Ile Lys His 
            100                 105                 110         


Ile Ser Val Thr Gly Ile Lys His Ile Ile Tyr Ala Lys Arg Tyr Asp 
        115                 120                 125             


Met Leu Ser Leu Glu Asp Glu Lys Asn Ile Tyr Tyr Tyr Cys Lys Lys 
    130                 135                 140                 


Leu Gly Ile Thr Ile Gln Gln Phe Asp Pro Asp Thr Leu Gly Ser Phe 
145                 150                 155                 160 


<210> 46
<211> 124
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-49 deaminase

<400> 46
Met Thr Ser Gly Ile Leu Lys Leu Ala Leu Glu Glu Ala Glu Ser Ser 
1               5                   10                  15      


Thr Tyr Arg Phe Arg Val Gly Ala Val Val Phe Lys Gly Ser Arg Ile 
            20                  25                  30          


Leu Ala Ser Gly His Asn Gly Ile Arg Ser Ser Arg Ile His Pro Leu 
        35                  40                  45              


His Lys Asn Tyr Asn Asn Ala Leu His Ala Glu Gln Asp Ala Ile Leu 
    50                  55                  60                  


Asn Val Lys Asp Trp Ser Thr Leu Lys Gly Cys Ser Ile Leu Val Val 
65                  70                  75                  80  


Lys Val Ser Lys Thr Lys Gly Thr Ile Ser Met Ala Lys Pro Cys Pro 
                85                  90                  95      


Met Cys Gln Gln Leu Leu Arg His Val Gly Ile Lys Thr Met Tyr Phe 
            100                 105                 110         


Ser Asn Glu Asn Gly Glu Ile Ile Ser Glu Lys Leu 
        115                 120                 


<210> 47
<211> 80
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG66 putative cytidine deaminase

<220>
<223> Description: MG66-50 deaminase

<400> 47
Met Val Asp Gly His Cys Val Asn Thr Val His Ala Glu Arg Gly Ala 
1               5                   10                  15      


Ile Arg Lys Met Thr Glu His Gly Ser Glu Tyr Thr Leu Tyr Val Thr 
            20                  25                  30          


His Tyr Pro Cys Arg Gly Cys Ala His Leu Ile Ser Ser Cys Pro Glu 
        35                  40                  45              


Ile Val Glu Val Val Tyr Leu Gly Asp Tyr Asn Asn Ser Ser Glu Ala 
    50                  55                  60                  


Thr Ala Leu Leu Met Gly Leu Ser Lys Gly Val His His Val Glu Gly 
65                  70                  75                  80  


<210> 48
<211> 150
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG67 putative cytidine deaminase

<220>
<223> Description: MG67-2 deaminase

<400> 48
Met Thr Ala Cys Lys Asp Lys Pro Val Cys Asn Val Ala Gln Ala Asp 
1               5                   10                  15      


Ala Thr Leu Leu Ser Thr Cys Leu Arg Leu Leu Gln Thr Ala His Glu 
            20                  25                  30          


Ile Lys Gln Asn Ala Tyr Ala Pro Tyr Ser Gly Phe Gln Val Gly Ala 
        35                  40                  45              


Ala Val Leu Ala Lys Asn Gly Ser Ile Ile Thr Gly Val Asn Val Glu 
    50                  55                  60                  


Asn Gly Ser Leu Gly Leu Thr Ile Cys Ala Glu Arg Thr Ala Leu Ser 
65                  70                  75                  80  


Lys Ala Val Ser Glu Gly Tyr Arg Pro Gly Asp Phe Val Ala Ile Ala 
                85                  90                  95      


Val Ala Ala Ser Ala Asp Gly Phe Ser Pro Cys Gly Ala Cys Arg Glu 
            100                 105                 110         


Val Ile Asn Glu Phe Gly Gln Asn Met Leu Val Val Phe Glu Tyr Gln 
        115                 120                 125             


Asp Glu Val Val Val Ala Pro Leu Lys Thr Leu Leu Ala Tyr Asn Phe 
    130                 135                 140                 


Val Lys Gly Lys Pro Ser 
145                 150 


<210> 49
<211> 62
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG67 putative cytdidine deaminase

<220>
<223> Description: MG67-4 deaminase

<400> 49
Met Ala Tyr Arg Pro Gly Asp Phe Val Ala Ile Ala Ile Ala Ala Ser 
1               5                   10                  15      


Ser Pro Asp Phe Ser Pro Cys Gly Ala Cys Arg Glu Val Ile Asn Glu 
            20                  25                  30          


Phe Gly Asp Asp Met Ala Val Ile Phe Glu Ile Lys Gly Glu Thr Val 
        35                  40                  45              


Val Ser Gln Leu Lys Glu His Leu Pro Tyr Asn Phe Lys Met 
    50                  55                  60          


<210> 50
<211> 250
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-1 deaminase

<400> 50
Met Gln His Val Lys His Met Arg Thr Ala Val Arg Leu Ala Arg Tyr 
1               5                   10                  15      


Ala Leu Asp His Asp Glu Thr Pro Val Ala Cys Ile Phe Val His Thr 
            20                  25                  30          


Pro Thr Gly Gln Val Met Ala Tyr Gly Met Asn Asp Thr Asn Lys Ser 
        35                  40                  45              


Leu Thr Gly Val Ala His Ala Glu Phe Met Gly Ile Asp Gln Ile Lys 
    50                  55                  60                  


Ala Met Leu Gly Ser Arg Gly Val Val Asp Val Phe Lys Asp Ile Thr 
65                  70                  75                  80  


Leu Tyr Val Thr Val Glu Pro Cys Ile Met Cys Ala Ser Ala Leu Lys 
                85                  90                  95      


Gln Leu Gly Ile Gly Lys Val Val Phe Gly Cys Gly Asn Glu Arg Phe 
            100                 105                 110         


Gly Gly Asn Gly Thr Val Leu Ser Val Asn His Asp Thr Cys Thr Leu 
        115                 120                 125             


Val Pro Lys Asn Asn Ser Ala Ala Gly Tyr Glu Ser Ile Pro Gly Ile 
    130                 135                 140                 


Leu Arg Lys Glu Ala Ile Met Leu Leu Arg Tyr Phe Tyr Val Arg Gln 
145                 150                 155                 160 


Asn Glu Arg Ala Pro Lys Pro Arg Ser Lys Ser Asp Arg Val Leu Asp 
                165                 170                 175     


Lys Asn Thr Phe Pro Pro Met Glu Trp Ser Lys Tyr Leu Asn Glu Glu 
            180                 185                 190         


Ala Phe Ile Glu Thr Phe Gly Asp Asp Tyr Lys Thr Cys Phe Ala Asn 
        195                 200                 205             


Lys Val Asp Leu Ser Ser Asn Ser Val Asp Trp Asp Leu Ile Asp Ser 
    210                 215                 220                 


His Gln Asp Asn Ile Ile Gln Glu Leu Glu Glu Gln Cys Lys Met Phe 
225                 230                 235                 240 


Arg Phe Asn Val His Lys Lys Ser Lys Val 
                245                 250 


<210> 51
<211> 239
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-2 deaminase

<400> 51
Met Leu Leu Asn Lys Arg Ser Tyr Gly His Phe Tyr Phe Ile Ala Leu 
1               5                   10                  15      


Met Thr Glu Phe Ser Lys Trp Asp Glu Tyr Tyr Met Lys Leu Ala Leu 
            20                  25                  30          


Met Glu Ala Glu Lys Ala Leu Asn Ser Asn Glu Val Pro Ile Gly Cys 
        35                  40                  45              


Val Phe Val Lys Ser Asp Thr Glu Val Glu Ile Pro Pro Leu Pro Ser 
    50                  55                  60                  


Arg Tyr Ser Lys Asp Glu Phe His Ser Ser Val Ile Ala Ser Gly Arg 
65                  70                  75                  80  


Asn Phe Thr Asn Glu Asn Tyr Asn Ala Thr His His Ala Glu Ile Met 
                85                  90                  95      


Ala Leu Thr Asp Leu Ile Ser Cys Leu Glu Cys Asn Cys Lys Lys Ile 
            100                 105                 110         


Asp Asn Met Gly Glu Pro Cys Lys Pro Thr Phe Ser Lys Asp Thr Glu 
        115                 120                 125             


Cys Pro Gln Cys Asn Phe Asn Leu Lys Asn Val Thr Leu Tyr Val Thr 
    130                 135                 140                 


Ile Glu Pro Cys Ile Met Cys Ile Ser Ala Leu Met Glu Leu Gln Val 
145                 150                 155                 160 


Ser Arg Ile Val Phe Gly Ala Asn Asn Asp Lys Phe Gly Gly Cys Gly 
                165                 170                 175     


Gly Thr Leu Thr Asn Val Pro Glu Ile Leu Lys Asp Thr Cys Ser Lys 
            180                 185                 190         


Asp Phe Glu Glu Ile Lys Ile Glu Arg Gly Leu Tyr Arg Glu Glu Ala 
        195                 200                 205             


Val Ile Leu Leu Arg Lys Phe Tyr Leu Ile Gln Asn Pro Lys Ala Pro 
    210                 215                 220                 


Asn Pro Lys Lys Lys Ala Lys Arg Thr Leu Asn Leu Ile Val Asp 
225                 230                 235                 


<210> 52
<211> 87
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG69 UGI

<220>
<223> Description: MG69-1 deaminase

<400> 52
Met Ser Lys Thr Ile Lys Asp Leu Glu Glu Phe Ala Ala Glu Lys Leu 
1               5                   10                  15      


Gly Tyr Asp Ile Val Phe Gln Glu Ser Ile Ser Met Glu Pro Gln Glu 
            20                  25                  30          


Val Glu His Leu Ile Thr Thr Asp Glu Lys Ser Val Leu Val Asn Ser 
        35                  40                  45              


Thr Tyr Asp Asp Asn Lys Glu Ile Thr Ile Ser Met Ile Ser Thr Pro 
    50                  55                  60                  


His Pro Glu Tyr Arg Pro Ile Ala Leu Ile Ile Gln Asp Met Glu Gly 
65                  70                  75                  80  


Glu Val Gln Arg Lys Glu Leu 
                85          


<210> 53
<211> 88
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG69 UGI

<220>
<223> Description: MG69-2 deaminase

<400> 53
Met Ser Lys Thr Ile Lys Asp Leu Glu Glu Phe Ala Ala Glu Lys Leu 
1               5                   10                  15      


Gly Tyr Asp Ile Val Phe Gln Glu Ser Ile Ser Met Glu Pro Gln Glu 
            20                  25                  30          


Val Glu His Leu Ile Thr Thr Asp Glu Lys Ser Val Leu Val Asn Ser 
        35                  40                  45              


Thr Tyr Asp Asp Asp Lys Glu Ile Thr Ile Ser Val Ile Ser Thr Pro 
    50                  55                  60                  


His Pro Glu Tyr Arg Pro Ile Ala Leu Val Ile Gln Asp Met Glu Gly 
65                  70                  75                  80  


Glu Val Gln Arg Lys Glu Leu Tyr 
                85              


<210> 54
<211> 84
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG69 UGI

<220>
<223> Description: MG69-3 deaminase

<400> 54
Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210> 55
<211> 67
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG69 UGI

<220>
<223> Description: MG69-4 deaminase

<400> 55
Met Ile Val Arg Val Thr Tyr Asn Val Glu Gln Glu Val Glu Ile Ser 
1               5                   10                  15      


Asn Lys Phe Asn Lys Asp Val Asp Ile Met Lys Pro Glu Glu Tyr Asp 
            20                  25                  30          


Asp Tyr Ile Asp Asp Leu Met Phe Ala Leu Asn Glu Lys Leu Pro Glu 
        35                  40                  45              


Tyr Ala Glu Ile Ser Cys Val Ile Asn Asp Asn Thr Gly Glu Val Leu 
    50                  55                  60                  


Phe Glu Gly 
65          


<210> 56
<211> 35
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG69 UGI

<220>
<223> Description: MG69-5 deaminase

<400> 56
Met Ser Lys Thr Ile Lys Asp Leu Glu Glu Phe Ala Ala Glu Lys Leu 
1               5                   10                  15      


Gly Tyr Asp Ile Val Phe Gln Glu Ser Ile Ser Met Glu Pro Gln Glu 
            20                  25                  30          


Val Glu His 
        35  


<210> 57
<211> 167
<212> PRT
<213> Escherichia coli

<220>
<223> Category: reference deaminase

<220>
<223> Description: P68398 TADA tRNA specific adenosine deaminase

<400> 57
Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp 
                165         


<210> 58
<211> 229
<212> PRT
<213> Rattus norvegicus

<220>
<223> Category: reference deaminase

<220>
<223> Description: P38483 APOBEC 1 C U editing deaminase

<400> 58
Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu Lys 
225                 


<210> 59
<211> 251
<212> PRT
<213> Heterocephalus glaber

<220>
<223> Category: reference deaminase

<220>
<223> Description: Aicda XM 004869540 cytidine deaminase

<400> 59
Met Ala Val Gly Ser Lys Pro Lys Ala Ala Leu Val Gly Pro His Trp 
1               5                   10                  15      


Glu Arg Glu Arg Ile Trp Cys Phe Leu Cys Ser Thr Gly Leu Gly Thr 
            20                  25                  30          


Gln Gln Thr Gly Gln Thr Ser Arg Trp Leu Arg Pro Ala Ala Thr Gln 
        35                  40                  45              


Asp Pro Val Ser Pro Pro Arg Ser Leu Leu Met Lys Gln Arg Lys Phe 
    50                  55                  60                  


Leu Tyr His Phe Lys Asn Val Arg Trp Ala Lys Gly Arg His Glu Thr 
65                  70                  75                  80  


Tyr Leu Cys Tyr Val Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser 
                85                  90                  95      


Leu Asp Phe Gly Tyr Leu Arg Asn Lys Ser Gly Cys His Val Glu Leu 
            100                 105                 110         


Leu Phe Leu Arg Tyr Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys 
        115                 120                 125             


Tyr Arg Val Thr Trp Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala 
    130                 135                 140                 


Arg His Val Ala Asp Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg 
145                 150                 155                 160 


Ile Phe Thr Ala Arg Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro 
                165                 170                 175     


Glu Gly Leu Arg Arg Leu His Arg Ala Gly Val Gln Ile Ala Ile Met 
            180                 185                 190         


Thr Phe Lys Asp Tyr Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His 
        195                 200                 205             


Glu Arg Thr Phe Lys Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg 
    210                 215                 220                 


Leu Ser Arg Gln Leu Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp 
225                 230                 235                 240 


Asp Leu Arg Asp Ala Phe Arg Thr Leu Gly Leu 
                245                 250     


<210> 60
<211> 224
<212> PRT
<213> Petromyzon marinus

<220>
<223> Category: reference deaminase

<220>
<223> Description: PmCDA1 L1 AVN88313.1 cytidine deaminase

<400> 60
Met Ala Gly Tyr Glu Cys Val Arg Val Ser Glu Lys Leu Asp Phe Asp 
1               5                   10                  15      


Thr Phe Glu Phe Gln Phe Glu Asn Leu His Tyr Ala Thr Glu Arg His 
            20                  25                  30          


Arg Thr Tyr Val Ile Phe Asp Val Lys Pro Gln Ser Ala Gly Gly Arg 
        35                  40                  45              


Ser Arg Arg Leu Trp Gly Tyr Ile Ile Asn Asn Pro Asn Val Cys His 
    50                  55                  60                  


Ala Glu Leu Ile Leu Met Ser Met Ile Asp Arg His Leu Glu Ser Asn 
65                  70                  75                  80  


Pro Gly Val Tyr Ala Met Thr Trp Tyr Met Ser Trp Ser Pro Cys Ala 
                85                  90                  95      


Asn Cys Ser Ser Lys Leu Asn Pro Trp Leu Lys Asn Leu Leu Glu Glu 
            100                 105                 110         


Gln Gly His Thr Leu Thr Met His Phe Ser Arg Ile Tyr Asp Arg Asp 
        115                 120                 125             


Arg Glu Gly Asp His Arg Gly Leu Arg Gly Leu Lys His Val Ser Asn 
    130                 135                 140                 


Ser Phe Arg Met Gly Val Val Gly Arg Ala Glu Val Lys Glu Cys Leu 
145                 150                 155                 160 


Ala Glu Tyr Val Glu Ala Ser Arg Arg Thr Leu Thr Trp Leu Asp Thr 
                165                 170                 175     


Thr Glu Ser Met Ala Ala Lys Met Arg Arg Lys Leu Phe Cys Ile Leu 
            180                 185                 190         


Val Arg Cys Ala Gly Met Arg Glu Ser Gly Ile Pro Leu His Leu Phe 
        195                 200                 205             


Thr Leu Gln Thr Pro Leu Leu Ser Gly Arg Val Val Trp Trp Arg Val 
    210                 215                 220                 


<210> 61
<211> 208
<212> PRT
<213> Petromyzon marinus

<220>
<223> Category: reference deaminase

<220>
<223> Description: PmCDA1 ABO15149.1 cytosine deaminase

<400> 61
Met Thr Asp Ala Glu Tyr Val Arg Ile His Glu Lys Leu Asp Ile Tyr 
1               5                   10                  15      


Thr Phe Lys Lys Gln Phe Phe Asn Asn Lys Lys Ser Val Ser His Arg 
            20                  25                  30          


Cys Tyr Val Leu Phe Glu Leu Lys Arg Arg Gly Glu Arg Arg Ala Cys 
        35                  40                  45              


Phe Trp Gly Tyr Ala Val Asn Lys Pro Gln Ser Gly Thr Glu Arg Gly 
    50                  55                  60                  


Ile His Ala Glu Ile Phe Ser Ile Arg Lys Val Glu Glu Tyr Leu Arg 
65                  70                  75                  80  


Asp Asn Pro Gly Gln Phe Thr Ile Asn Trp Tyr Ser Ser Trp Ser Pro 
                85                  90                  95      


Cys Ala Asp Cys Ala Glu Lys Ile Leu Glu Trp Tyr Asn Gln Glu Leu 
            100                 105                 110         


Arg Gly Asn Gly His Thr Leu Lys Ile Trp Ala Cys Lys Leu Tyr Tyr 
        115                 120                 125             


Glu Lys Asn Ala Arg Asn Gln Ile Gly Leu Trp Asn Leu Arg Asp Asn 
    130                 135                 140                 


Gly Val Gly Leu Asn Val Met Val Ser Glu His Tyr Gln Cys Cys Arg 
145                 150                 155                 160 


Lys Ile Phe Ile Gln Ser Ser His Asn Gln Leu Asn Glu Asn Arg Trp 
                165                 170                 175     


Leu Glu Lys Thr Leu Lys Arg Ala Glu Lys Arg Arg Ser Glu Leu Ser 
            180                 185                 190         


Ile Met Ile Gln Val Lys Ile Leu His Thr Thr Lys Ser Pro Ala Val 
        195                 200                 205             


<210> 62
<211> 199
<212> PRT
<213> Homo sapiens

<220>
<223> Category: reference deaminase

<220>
<223> Description: NP 663745.1 DNA dC- dU-editing deaminase
      APOBEC-3A isoform a

<400> 62
Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His 
1               5                   10                  15      


Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr 
            20                  25                  30          


Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met 
        35                  40                  45              


Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys 
    50                  55                  60                  


Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro 
65                  70                  75                  80  


Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile 
                85                  90                  95      


Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala 
            100                 105                 110         


Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg 
        115                 120                 125             


Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg 
    130                 135                 140                 


Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His 
145                 150                 155                 160 


Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp 
                165                 170                 175     


Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala 
            180                 185                 190         


Ile Leu Gln Asn Gln Gly Asn 
        195                 


<210> 63
<211> 198
<212> PRT
<213> Homo sapiens

<220>
<223> Category: reference deaminase

<220>
<223> Description: Q9GZX7.1 AICDA Single-stranded DNA cytosine 
      deaminase (Activation-induced cytidine deaminase, Cytidine
      aminohydrolase)

<400> 63
Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210> 64
<211> 197
<212> PRT
<213> Lampetra planeri

<220>
<223> Category: reference deaminase

<220>
<223> Description: LpCDA1L1 3 AVN88320.1 cytidine deaminase

<400> 64
Met Ala Gly Asp Glu Asn Val Arg Val Ser Glu Lys Leu His Phe Asn 
1               5                   10                  15      


Thr Phe Glu Phe Glu Phe Glu Asn Leu His Tyr Ala Glu Gly Arg Gly 
            20                  25                  30          


Gln Thr Tyr Val Ile Phe Asp Val Lys Pro Gln Ser Glu Gly Gly Arg 
        35                  40                  45              


Gly Glu Arg Leu Trp Gly Tyr Val Arg Asn Lys Pro Leu Gly Asp His 
    50                  55                  60                  


Ala Glu Val Ile Leu Met Ser Lys Ile Asn Asp His Leu Glu Thr His 
65                  70                  75                  80  


Gln Asp Asn Tyr Thr Met Thr Trp Tyr Met Ser Trp Ser Pro Cys Gly 
                85                  90                  95      


Lys Cys Ser Ser Glu Leu Val Pro Trp Leu Gln Asn Leu Leu Lys Lys 
            100                 105                 110         


Gln His Lys Leu Thr Met His Phe Ser Arg Ile Tyr Asp Lys Asp Arg 
        115                 120                 125             


Ala Val Asp His Arg Gly Leu Cys Asp Leu Gln His Val Val Ser Asn 
    130                 135                 140                 


Gly Phe Gln Met Gly Val Met Gly Gln Thr Glu Val Asp Thr Cys Leu 
145                 150                 155                 160 


Ala Glu Tyr Val Glu Ala Ser Gly Cys Pro Pro Leu Lys Trp Leu His 
                165                 170                 175     


Met Thr Asp Ser Asn Ala Thr Gln Met Gln Asp Lys Leu Ser Ser Ile 
            180                 185                 190         


Leu Met Asn Arg Phe 
        195         


<210> 65
<211> 198
<212> PRT
<213> Lampetra planeri

<220>
<223> Category: reference deaminase

<220>
<223> Description: LpCDA1L1 1 AVN88319.1 cytidine deaminase

<400> 65
Met Ala Gly Asp Glu Asn Val Arg Val Ser Lys Lys Leu Asp Phe Asn 
1               5                   10                  15      


Thr Phe Glu Phe Glu Phe Glu Asn Leu His Tyr Ala Glu Gly Arg Gly 
            20                  25                  30          


Arg Thr Tyr Val Ile Phe Asp Val Lys Pro Gln Ser Glu Gly Gly Arg 
        35                  40                  45              


Gly Glu Arg Leu Trp Gly Tyr Val Arg Asn Asn Pro Leu Asp Asp His 
    50                  55                  60                  


Ala Glu Val Ile Leu Met Ser Lys Ile Asn Asp His Leu Glu Thr His 
65                  70                  75                  80  


Gln Gly Asn Tyr Thr Met Thr Trp Tyr Met Ser Trp Ser Pro Cys Gly 
                85                  90                  95      


Asn Cys Ser Ser Glu Leu Val Pro Trp Leu Gln Asn Leu Leu Glu Glu 
            100                 105                 110         


Gln Gln His Thr Leu Thr Met Tyr Phe Ser Arg Ile Tyr Asp Lys Asp 
        115                 120                 125             


Arg Ala Val Asp His Arg Gly Leu Cys Asp Leu Gln His Val Val Ser 
    130                 135                 140                 


Asn Gly Phe Gln Met Gly Val Met Gly Gln Thr Glu Val Asp Thr Cys 
145                 150                 155                 160 


Leu Ala Glu Tyr Val Glu Ala Ser Gly Cys Pro Pro Leu Lys Trp Leu 
                165                 170                 175     


His Met Thr Asp Ser Asn Ala Thr Gln Thr Gln Asp Lys Leu Ser Ser 
            180                 185                 190         


Ile Leu Met Asn Arg Phe 
        195             


<210> 66
<211> 642
<212> DNA
<213> Lampetra planeri

<220>
<223> Category: reference deaminase

<220>
<223> Description: ljCDA1 cytidine deaminase

<400> 66
gccaacgccg agtacgtgcg cgtgggcgag aagctggaca gctgcacctt ccgcacccag       60

ttcctgaact accgccgcag ccgcagccgc cgctgctgcg tgatcttcga gctgaagcgc      120

cagaacagcc gcgtgcgctt ctggggctac gccatgaaca agccctggag caacgccgac      180

gtgggcatcc acgccgagtt cttctgcatc aagaaggtga agaagtacct gcgcaagaac      240

cccggcatct acaccatcaa ctggtacagc agctggagcc cctgcgccaa ctgcgccgag      300

aagatcctga actggtacaa caagaagctg atgggcaagg gccacaccct gaagatttgg      360

gcctgcaagc tgtacttcga gaacatcaag cgcaaccaga tcggcctgtg gaacctgcgc      420

aacaacggcg tgggcctggc catcatgctg ggcgagcact accagtggtg ctggaacaac      480

tacatccaga ccctgggccg caacctgaac gagaacaagt ggctgaaaaa gaccagcaac      540

cgcgcccgca cccgccgcag cgagctgagc atcatgatcc acgtgaagcg cctgcacacc      600

gcccgcctgc tgctgttcaa gcgcctgtgc ggctggttca gc                         642


<210> 67
<211> 84
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      Bacillus phage PBS2 sequence

<220>
<223> Category: reference UGI

<220>
<223> Description: P14739 UNGI BPPB2 (UGI)

<400> 67
Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210> 68
<211> 1245
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: linker-His tag-adenine deaminse-linker-nickase
      -linker-SV40 NLS

<400> 68
Met Gly Ser Ser His His His His His His Ser Glu Val Glu Phe Ser 
1               5                   10                  15      


His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg 
            20                  25                  30          


Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg 
        35                  40                  45              


Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr 
    50                  55                  60                  


Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln 
65                  70                  75                  80  


Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Ser Thr Phe Glu Pro Cys 
                85                  90                  95      


Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val 
            100                 105                 110         


Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp 
        115                 120                 125             


Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly 
    130                 135                 140                 


Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met 
145                 150                 155                 160 


Pro Arg Arg Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp 
                165                 170                 175     


Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
            180                 185                 190         


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
        195                 200                 205             


Met Lys Lys Lys Val Phe Ala Phe Ala Leu Gly Lys Ala Ser Ile Gly 
    210                 215                 220                 


Tyr Cys Val Arg Asp Gly Phe Asn Ile Asn Glu Ala Asn Ser Ile Ile 
225                 230                 235                 240 


Ile Asp Lys Asp His Ala Glu Thr Met Ser Leu Arg Asp Arg Arg Arg 
                245                 250                 255     


Ile Lys Lys Thr Leu Asp Ala His His Ala Arg Glu Glu Tyr Phe Asn 
            260                 265                 270         


Lys Val Trp Ser Gly Val Gly Leu Asn Ile Leu Asp Lys Asn Asn Asn 
        275                 280                 285             


Asn Phe Lys Lys Glu Phe Pro Ser Lys Asn Glu Asp Val Ile Tyr Thr 
    290                 295                 300                 


Ser Cys Leu Leu Arg Ile Ala Leu Leu Gln Asn Gln Lys Leu Glu Glu 
305                 310                 315                 320 


Trp Gln Ile Tyr Lys Ala Leu Tyr Asn Ala Phe Gln Arg Arg Gly Tyr 
                325                 330                 335     


Asp Val Asn Ile Ala Trp Lys Ser Val Gln Thr Asp Asp Asp Lys Glu 
            340                 345                 350         


Asn Ile Glu Arg Met Lys Lys Tyr Thr Cys Glu Asn Asp Ile Glu Leu 
        355                 360                 365             


Ile Ile Ser Asp Glu Tyr Lys Tyr Pro Cys Tyr Tyr Asp Ala Leu Arg 
    370                 375                 380                 


Leu Gly Leu Trp Asn Glu Asp Glu Pro Thr Lys Leu Asn Arg Ser Ile 
385                 390                 395                 400 


Pro Gln His Asn Ser Asn Lys Val Arg Thr Thr Glu Tyr Val Ala Pro 
                405                 410                 415     


Arg Glu Leu Val Glu Lys Glu Leu Lys Gln Leu Trp Leu Asn Ala Gln 
            420                 425                 430         


Leu Gln Leu Pro Gln Leu Asn Ser Ile Ser Ala Glu Glu Phe Leu Tyr 
        435                 440                 445             


Gly Glu Tyr Lys Ile Pro Tyr Gly Ser Cys Val Gln Lys Glu Phe Lys 
    450                 455                 460                 


Gln Tyr Arg Gly Thr Lys Lys Asp Trp Gln Gly Val Leu Cys Gln Lys 
465                 470                 475                 480 


Ile Pro Arg Phe Asp Asn Arg Ile Ile Ala Lys Cys Lys Leu Leu Pro 
                485                 490                 495     


Lys Arg Asn Val Cys Lys Ala Asn Thr Ile Glu Asn Val Ser Phe Val 
            500                 505                 510         


Leu Leu Met Lys Leu Lys Asn Leu Arg Phe Thr Val Ile Ser Gly Glu 
        515                 520                 525             


Lys Leu Arg Leu Ser Pro Val Gln Ile Lys Gln Ile Tyr Glu Asn Trp 
    530                 535                 540                 


Leu Glu Lys Val Glu Asp Thr Tyr Asn Lys Asp Leu Glu Lys Ala Glu 
545                 550                 555                 560 


Lys Leu Gly Lys Glu Asn Pro Glu Lys Arg Leu Asn Ile Thr Ile Thr 
                565                 570                 575     


Arg Gln Glu Ile Glu Lys Ile Ile Gly Glu Lys Ile Ile Asp Lys Ile 
            580                 585                 590         


Glu Pro Met Lys Ala Asp Ile Ser Gly Arg Ser Ser Phe Cys Arg Arg 
        595                 600                 605             


Ala Cys Gln Ile Met Asn Lys Ile Ile Leu Asp Gly Glu Leu Tyr Pro 
    610                 615                 620                 


Gln Asn Val Asp Ile Ser Glu Phe Val Asp Arg Asp Asn Ala Lys Asn 
625                 630                 635                 640 


Gly Ile Thr Glu Glu Glu Ile Gln Ser Met Leu Ser Lys Ile Gly Asp 
                645                 650                 655     


Trp Asn Asn Leu Tyr Ile Pro Asp Asn Arg Glu Glu Asn Ala Gln Asn 
            660                 665                 670         


Ala Ser Asn Ser Arg Ile Lys Thr Asp Ile Met Leu Gly Asn Ile Thr 
        675                 680                 685             


Asn Pro Ile Val Arg Asn Arg Leu Gln Ile Phe Arg Asp Leu Leu Leu 
    690                 695                 700                 


Asn Leu Ala Glu Lys Tyr Gly Thr Pro Asp Glu Val Ile Phe Glu Phe 
705                 710                 715                 720 


Val Arg Asp Gly Ala Asp Asn Ser Leu Tyr Gly Ser Lys Lys Ala Gln 
                725                 730                 735     


Asp Thr Glu Arg Phe Met Lys Gln Gln Glu Lys Glu Asn Glu Gln Ile 
            740                 745                 750         


Ile Asn Glu Leu Lys Asp Ala Gly Leu Tyr Val Asn Gly Gln Asn His 
        755                 760                 765             


Ala Asn Ala Thr Tyr Phe Leu Met His Lys Leu Leu Lys Ser Gln Gly 
    770                 775                 780                 


Gly Lys Cys Ile Tyr Ser Gly Gln Asn Ile Ser Pro Ser Asn Tyr Asp 
785                 790                 795                 800 


Glu Cys Glu Ile Asp His Ile Tyr Pro Arg Thr Leu Gly Gly Asn Asp 
                805                 810                 815     


Ala Leu Tyr Asn Lys Val Val Cys Tyr Arg Arg Glu Asn Gln Asp Lys 
            820                 825                 830         


Lys Gly Arg Thr Pro Tyr Glu Trp Leu Ser Pro Asp Ser Asp Lys Trp 
        835                 840                 845             


Ala Asn Tyr Val Asn Arg Leu Asn Lys Ile Lys Lys Gln Leu Gly Lys 
    850                 855                 860                 


Lys Lys Phe Glu Leu Leu Thr Ser Lys Pro Glu Asp Cys Glu Lys Leu 
865                 870                 875                 880 


Ile Glu Ser Tyr Asn Gly Leu Ala Glu Thr Ser His Ile Ala Arg Val 
                885                 890                 895     


Ala Gln Gln Met Thr Ala Phe Ile Phe Gly Trp Gly Leu Gln Ile Gln 
            900                 905                 910         


Gly Glu Ser Arg Arg Ile Phe Val Asn Asn Gly Ser Ser Thr Cys Ala 
        915                 920                 925             


Ile Arg Lys Arg Tyr Gly Leu Asn Lys Leu Leu Gly Asp Asn Leu Lys 
    930                 935                 940                 


Lys Asn Arg Ser Asn Asp Lys His His Ala Leu Asp Ala Ile Cys Ile 
945                 950                 955                 960 


Ser Phe Ser Arg Asp Phe Lys Tyr Asp Lys Glu Ile Lys Lys Asp Ile 
                965                 970                 975     


Ile Lys Gly Phe Asn Pro Glu Ile Val Lys Asn Ala Ile Asp Lys Ile 
            980                 985                 990         


Met Pro Tyr Pro Tyr Ala Asn Asp  Lys Pro Phe Lys Gly  Asn Thr Lys 
        995                 1000                 1005             


Pro Leu  Glu Thr Ile Tyr Gly  Leu Arg Thr Tyr Gly  Asp Lys Ser 
    1010                 1015                 1020             


Tyr Ile  Thr Gln Arg Val Glu  Leu Asn Ser Ile Asp  Lys Lys Ala 
    1025                 1030                 1035             


Thr Lys  Ile Lys Ser Ile Ile  Asp Glu Thr Ile Lys  Asn Asp Leu 
    1040                 1045                 1050             


Leu Asn  Lys Leu Lys Glu Asn  Pro Thr Glu Gln Glu  Trp Lys Leu 
    1055                 1060                 1065             


Met Leu  Gln Asn Tyr Ile His  Pro Lys Lys Gln Thr  Lys Val Lys 
    1070                 1075                 1080             


Lys Val  Met Ile Ser Val Ser  Glu Gly Glu Ile Thr  Lys Asp Ser 
    1085                 1090                 1095             


Asn Asn  Arg Glu Arg Met Gly  Glu Phe Val Asp Phe  Gly Thr Lys 
    1100                 1105                 1110             


Gly Thr  Gln His Gln Phe Lys  His Ser Lys Arg His  Lys Gly Gln 
    1115                 1120                 1125             


Ile Leu  Tyr Phe Asn Glu Lys  Gly Val Val Glu Val  Met Pro Val 
    1130                 1135                 1140             


Tyr Ser  Asn Ile Lys Thr Thr  Asp Val Lys Asp Lys  Leu Gln Asn 
    1145                 1150                 1155             


Met Gly  Cys Lys Leu Tyr Asn  Lys Gly Gln Met Phe  Tyr Ser Gly 
    1160                 1165                 1170             


Cys Leu  Val Asp Ile Pro Lys  Pro Phe Lys Ala Gly  Ser Lys Glu 
    1175                 1180                 1185             


Tyr Pro  Ala Gly Arg Tyr Gln  Ile Lys Thr Ile Arg  Ser Asp Lys 
    1190                 1195                 1200             


Val Ala  Glu Leu Glu Asp Ala  Cys Gly Asn Lys Ile  Ser Thr Asn 
    1205                 1210                 1215             


Val Lys  Tyr Leu Val Pro Ala  Glu Phe Lys Lys Val  Glu Ser Lys 
    1220                 1225                 1230             


Gly Ser  Gly Gly Ser Pro Lys  Lys Lys Arg Lys Val  
    1235                 1240                 1245 


<210> 69
<211> 1380
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: linker-His tag-cytidine deaminase-linker-nickase
      -linker-uracil glycosylase inhibitor-linker-SV40 NLS

<400> 69
Met Gly Ser Ser His His His His His His Met Ser Ser Glu Thr Gly 
1               5                   10                  15      


Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu 
            20                  25                  30          


Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu 
        35                  40                  45              


Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr 
    50                  55                  60                  


Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe 
65                  70                  75                  80  


Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp 
                85                  90                  95      


Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu 
            100                 105                 110         


Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg 
        115                 120                 125             


Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu 
    130                 135                 140                 


Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly 
145                 150                 155                 160 


Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His 
                165                 170                 175     


Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu 
            180                 185                 190         


Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg 
        195                 200                 205             


Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His 
    210                 215                 220                 


Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Ser 
225                 230                 235                 240 


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ala 
                245                 250                 255     


Met Lys Lys Lys Val Phe Ala Phe Ala Leu Gly Lys Ala Ser Ile Gly 
            260                 265                 270         


Tyr Cys Val Arg Asp Gly Phe Asn Ile Asn Glu Ala Asn Ser Ile Ile 
        275                 280                 285             


Ile Asp Lys Asp His Ala Glu Thr Met Ser Leu Arg Asp Arg Arg Arg 
    290                 295                 300                 


Ile Lys Lys Thr Leu Asp Ala His His Ala Arg Glu Glu Tyr Phe Asn 
305                 310                 315                 320 


Lys Val Trp Ser Gly Val Gly Leu Asn Ile Leu Asp Lys Asn Asn Asn 
                325                 330                 335     


Asn Phe Lys Lys Glu Phe Pro Ser Lys Asn Glu Asp Val Ile Tyr Thr 
            340                 345                 350         


Ser Cys Leu Leu Arg Ile Ala Leu Leu Gln Asn Gln Lys Leu Glu Glu 
        355                 360                 365             


Trp Gln Ile Tyr Lys Ala Leu Tyr Asn Ala Phe Gln Arg Arg Gly Tyr 
    370                 375                 380                 


Asp Val Asn Ile Ala Trp Lys Ser Val Gln Thr Asp Asp Asp Lys Glu 
385                 390                 395                 400 


Asn Ile Glu Arg Met Lys Lys Tyr Thr Cys Glu Asn Asp Ile Glu Leu 
                405                 410                 415     


Ile Ile Ser Asp Glu Tyr Lys Tyr Pro Cys Tyr Tyr Asp Ala Leu Arg 
            420                 425                 430         


Leu Gly Leu Trp Asn Glu Asp Glu Pro Thr Lys Leu Asn Arg Ser Ile 
        435                 440                 445             


Pro Gln His Asn Ser Asn Lys Val Arg Thr Thr Glu Tyr Val Ala Pro 
    450                 455                 460                 


Arg Glu Leu Val Glu Lys Glu Leu Lys Gln Leu Trp Leu Asn Ala Gln 
465                 470                 475                 480 


Leu Gln Leu Pro Gln Leu Asn Ser Ile Ser Ala Glu Glu Phe Leu Tyr 
                485                 490                 495     


Gly Glu Tyr Lys Ile Pro Tyr Gly Ser Cys Val Gln Lys Glu Phe Lys 
            500                 505                 510         


Gln Tyr Arg Gly Thr Lys Lys Asp Trp Gln Gly Val Leu Cys Gln Lys 
        515                 520                 525             


Ile Pro Arg Phe Asp Asn Arg Ile Ile Ala Lys Cys Lys Leu Leu Pro 
    530                 535                 540                 


Lys Arg Asn Val Cys Lys Ala Asn Thr Ile Glu Asn Val Ser Phe Val 
545                 550                 555                 560 


Leu Leu Met Lys Leu Lys Asn Leu Arg Phe Thr Val Ile Ser Gly Glu 
                565                 570                 575     


Lys Leu Arg Leu Ser Pro Val Gln Ile Lys Gln Ile Tyr Glu Asn Trp 
            580                 585                 590         


Leu Glu Lys Val Glu Asp Thr Tyr Asn Lys Asp Leu Glu Lys Ala Glu 
        595                 600                 605             


Lys Leu Gly Lys Glu Asn Pro Glu Lys Arg Leu Asn Ile Thr Ile Thr 
    610                 615                 620                 


Arg Gln Glu Ile Glu Lys Ile Ile Gly Glu Lys Ile Ile Asp Lys Ile 
625                 630                 635                 640 


Glu Pro Met Lys Ala Asp Ile Ser Gly Arg Ser Ser Phe Cys Arg Arg 
                645                 650                 655     


Ala Cys Gln Ile Met Asn Lys Ile Ile Leu Asp Gly Glu Leu Tyr Pro 
            660                 665                 670         


Gln Asn Val Asp Ile Ser Glu Phe Val Asp Arg Asp Asn Ala Lys Asn 
        675                 680                 685             


Gly Ile Thr Glu Glu Glu Ile Gln Ser Met Leu Ser Lys Ile Gly Asp 
    690                 695                 700                 


Trp Asn Asn Leu Tyr Ile Pro Asp Asn Arg Glu Glu Asn Ala Gln Asn 
705                 710                 715                 720 


Ala Ser Asn Ser Arg Ile Lys Thr Asp Ile Met Leu Gly Asn Ile Thr 
                725                 730                 735     


Asn Pro Ile Val Arg Asn Arg Leu Gln Ile Phe Arg Asp Leu Leu Leu 
            740                 745                 750         


Asn Leu Ala Glu Lys Tyr Gly Thr Pro Asp Glu Val Ile Phe Glu Phe 
        755                 760                 765             


Val Arg Asp Gly Ala Asp Asn Ser Leu Tyr Gly Ser Lys Lys Ala Gln 
    770                 775                 780                 


Asp Thr Glu Arg Phe Met Lys Gln Gln Glu Lys Glu Asn Glu Gln Ile 
785                 790                 795                 800 


Ile Asn Glu Leu Lys Asp Ala Gly Leu Tyr Val Asn Gly Gln Asn His 
                805                 810                 815     


Ala Asn Ala Thr Tyr Phe Leu Met His Lys Leu Leu Lys Ser Gln Gly 
            820                 825                 830         


Gly Lys Cys Ile Tyr Ser Gly Gln Asn Ile Ser Pro Ser Asn Tyr Asp 
        835                 840                 845             


Glu Cys Glu Ile Asp His Ile Tyr Pro Arg Thr Leu Gly Gly Asn Asp 
    850                 855                 860                 


Ala Leu Tyr Asn Lys Val Val Cys Tyr Arg Arg Glu Asn Gln Asp Lys 
865                 870                 875                 880 


Lys Gly Arg Thr Pro Tyr Glu Trp Leu Ser Pro Asp Ser Asp Lys Trp 
                885                 890                 895     


Ala Asn Tyr Val Asn Arg Leu Asn Lys Ile Lys Lys Gln Leu Gly Lys 
            900                 905                 910         


Lys Lys Phe Glu Leu Leu Thr Ser Lys Pro Glu Asp Cys Glu Lys Leu 
        915                 920                 925             


Ile Glu Ser Tyr Asn Gly Leu Ala Glu Thr Ser His Ile Ala Arg Val 
    930                 935                 940                 


Ala Gln Gln Met Thr Ala Phe Ile Phe Gly Trp Gly Leu Gln Ile Gln 
945                 950                 955                 960 


Gly Glu Ser Arg Arg Ile Phe Val Asn Asn Gly Ser Ser Thr Cys Ala 
                965                 970                 975     


Ile Arg Lys Arg Tyr Gly Leu Asn Lys Leu Leu Gly Asp Asn Leu Lys 
            980                 985                 990         


Lys Asn Arg Ser Asn Asp Lys His  His Ala Leu Asp Ala  Ile Cys Ile 
        995                 1000                 1005             


Ser Phe  Ser Arg Asp Phe Lys  Tyr Asp Lys Glu Ile  Lys Lys Asp 
    1010                 1015                 1020             


Ile Ile  Lys Gly Phe Asn Pro  Glu Ile Val Lys Asn  Ala Ile Asp 
    1025                 1030                 1035             


Lys Ile  Met Pro Tyr Pro Tyr  Ala Asn Asp Lys Pro  Phe Lys Gly 
    1040                 1045                 1050             


Asn Thr  Lys Pro Leu Glu Thr  Ile Tyr Gly Leu Arg  Thr Tyr Gly 
    1055                 1060                 1065             


Asp Lys  Ser Tyr Ile Thr Gln  Arg Val Glu Leu Asn  Ser Ile Asp 
    1070                 1075                 1080             


Lys Lys  Ala Thr Lys Ile Lys  Ser Ile Ile Asp Glu  Thr Ile Lys 
    1085                 1090                 1095             


Asn Asp  Leu Leu Asn Lys Leu  Lys Glu Asn Pro Thr  Glu Gln Glu 
    1100                 1105                 1110             


Trp Lys  Leu Met Leu Gln Asn  Tyr Ile His Pro Lys  Lys Gln Thr 
    1115                 1120                 1125             


Lys Val  Lys Lys Val Met Ile  Ser Val Ser Glu Gly  Glu Ile Thr 
    1130                 1135                 1140             


Lys Asp  Ser Asn Asn Arg Glu  Arg Met Gly Glu Phe  Val Asp Phe 
    1145                 1150                 1155             


Gly Thr  Lys Gly Thr Gln His  Gln Phe Lys His Ser  Lys Arg His 
    1160                 1165                 1170             


Lys Gly  Gln Ile Leu Tyr Phe  Asn Glu Lys Gly Val  Val Glu Val 
    1175                 1180                 1185             


Met Pro  Val Tyr Ser Asn Ile  Lys Thr Thr Asp Val  Lys Asp Lys 
    1190                 1195                 1200             


Leu Gln  Asn Met Gly Cys Lys  Leu Tyr Asn Lys Gly  Gln Met Phe 
    1205                 1210                 1215             


Tyr Ser  Gly Cys Leu Val Asp  Ile Pro Lys Pro Phe  Lys Ala Gly 
    1220                 1225                 1230             


Ser Lys  Glu Tyr Pro Ala Gly  Arg Tyr Gln Ile Lys  Thr Ile Arg 
    1235                 1240                 1245             


Ser Asp  Lys Val Ala Glu Leu  Glu Asp Ala Cys Gly  Asn Lys Ile 
    1250                 1255                 1260             


Ser Thr  Asn Val Lys Tyr Leu  Val Pro Ala Glu Phe  Lys Lys Val 
    1265                 1270                 1275             


Glu Ser  Lys Gly Ser Gly Gly  Ser Thr Asn Leu Ser  Asp Ile Ile 
    1280                 1285                 1290             


Glu Lys  Glu Thr Gly Lys Gln  Leu Val Ile Gln Glu  Ser Ile Leu 
    1295                 1300                 1305             


Met Leu  Pro Glu Glu Val Glu  Glu Val Ile Gly Asn  Lys Pro Glu 
    1310                 1315                 1320             


Ser Asp  Ile Leu Val His Thr  Ala Tyr Asp Glu Ser  Thr Asp Glu 
    1325                 1330                 1335             


Asn Val  Met Leu Leu Thr Ser  Asp Ala Pro Glu Tyr  Lys Pro Trp 
    1340                 1345                 1350             


Ala Leu  Val Ile Gln Asp Ser  Asn Gly Glu Asn Lys  Ile Lys Met 
    1355                 1360                 1365             


Leu Ser  Gly Gly Ser Pro Lys  Lys Lys Arg Lys Val  
    1370                 1375                 1380 


<210> 70
<211> 1025
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG1-4 (D9A) nickase

<400> 70
Met Lys Lys Lys Val Phe Ala Phe Ala Leu Gly Lys Ala Ser Ile Gly 
1               5                   10                  15      


Tyr Cys Val Arg Asp Gly Phe Asn Ile Asn Glu Ala Asn Ser Ile Ile 
            20                  25                  30          


Ile Asp Lys Asp His Ala Glu Thr Met Ser Leu Arg Asp Arg Arg Arg 
        35                  40                  45              


Ile Lys Lys Thr Leu Asp Ala His His Ala Arg Glu Glu Tyr Phe Asn 
    50                  55                  60                  


Lys Val Trp Ser Gly Val Gly Leu Asn Ile Leu Asp Lys Asn Asn Asn 
65                  70                  75                  80  


Asn Phe Lys Lys Glu Phe Pro Ser Lys Asn Glu Asp Val Ile Tyr Thr 
                85                  90                  95      


Ser Cys Leu Leu Arg Ile Ala Leu Leu Gln Asn Gln Lys Leu Glu Glu 
            100                 105                 110         


Trp Gln Ile Tyr Lys Ala Leu Tyr Asn Ala Phe Gln Arg Arg Gly Tyr 
        115                 120                 125             


Asp Val Asn Ile Ala Trp Lys Ser Val Gln Thr Asp Asp Asp Lys Glu 
    130                 135                 140                 


Asn Ile Glu Arg Met Lys Lys Tyr Thr Cys Glu Asn Asp Ile Glu Leu 
145                 150                 155                 160 


Ile Ile Ser Asp Glu Tyr Lys Tyr Pro Cys Tyr Tyr Asp Ala Leu Arg 
                165                 170                 175     


Leu Gly Leu Trp Asn Glu Asp Glu Pro Thr Lys Leu Asn Arg Ser Ile 
            180                 185                 190         


Pro Gln His Asn Ser Asn Lys Val Arg Thr Thr Glu Tyr Val Ala Pro 
        195                 200                 205             


Arg Glu Leu Val Glu Lys Glu Leu Lys Gln Leu Trp Leu Asn Ala Gln 
    210                 215                 220                 


Leu Gln Leu Pro Gln Leu Asn Ser Ile Ser Ala Glu Glu Phe Leu Tyr 
225                 230                 235                 240 


Gly Glu Tyr Lys Ile Pro Tyr Gly Ser Cys Val Gln Lys Glu Phe Lys 
                245                 250                 255     


Gln Tyr Arg Gly Thr Lys Lys Asp Trp Gln Gly Val Leu Cys Gln Lys 
            260                 265                 270         


Ile Pro Arg Phe Asp Asn Arg Ile Ile Ala Lys Cys Lys Leu Leu Pro 
        275                 280                 285             


Lys Arg Asn Val Cys Lys Ala Asn Thr Ile Glu Asn Val Ser Phe Val 
    290                 295                 300                 


Leu Leu Met Lys Leu Lys Asn Leu Arg Phe Thr Val Ile Ser Gly Glu 
305                 310                 315                 320 


Lys Leu Arg Leu Ser Pro Val Gln Ile Lys Gln Ile Tyr Glu Asn Trp 
                325                 330                 335     


Leu Glu Lys Val Glu Asp Thr Tyr Asn Lys Asp Leu Glu Lys Ala Glu 
            340                 345                 350         


Lys Leu Gly Lys Glu Asn Pro Glu Lys Arg Leu Asn Ile Thr Ile Thr 
        355                 360                 365             


Arg Gln Glu Ile Glu Lys Ile Ile Gly Glu Lys Ile Ile Asp Lys Ile 
    370                 375                 380                 


Glu Pro Met Lys Ala Asp Ile Ser Gly Arg Ser Ser Phe Cys Arg Arg 
385                 390                 395                 400 


Ala Cys Gln Ile Met Asn Lys Ile Ile Leu Asp Gly Glu Leu Tyr Pro 
                405                 410                 415     


Gln Asn Val Asp Ile Ser Glu Phe Val Asp Arg Asp Asn Ala Lys Asn 
            420                 425                 430         


Gly Ile Thr Glu Glu Glu Ile Gln Ser Met Leu Ser Lys Ile Gly Asp 
        435                 440                 445             


Trp Asn Asn Leu Tyr Ile Pro Asp Asn Arg Glu Glu Asn Ala Gln Asn 
    450                 455                 460                 


Ala Ser Asn Ser Arg Ile Lys Thr Asp Ile Met Leu Gly Asn Ile Thr 
465                 470                 475                 480 


Asn Pro Ile Val Arg Asn Arg Leu Gln Ile Phe Arg Asp Leu Leu Leu 
                485                 490                 495     


Asn Leu Ala Glu Lys Tyr Gly Thr Pro Asp Glu Val Ile Phe Glu Phe 
            500                 505                 510         


Val Arg Asp Gly Ala Asp Asn Ser Leu Tyr Gly Ser Lys Lys Ala Gln 
        515                 520                 525             


Asp Thr Glu Arg Phe Met Lys Gln Gln Glu Lys Glu Asn Glu Gln Ile 
    530                 535                 540                 


Ile Asn Glu Leu Lys Asp Ala Gly Leu Tyr Val Asn Gly Gln Asn His 
545                 550                 555                 560 


Ala Asn Ala Thr Tyr Phe Leu Met His Lys Leu Leu Lys Ser Gln Gly 
                565                 570                 575     


Gly Lys Cys Ile Tyr Ser Gly Gln Asn Ile Ser Pro Ser Asn Tyr Asp 
            580                 585                 590         


Glu Cys Glu Ile Asp His Ile Tyr Pro Arg Thr Leu Gly Gly Asn Asp 
        595                 600                 605             


Ala Leu Tyr Asn Lys Val Val Cys Tyr Arg Arg Glu Asn Gln Asp Lys 
    610                 615                 620                 


Lys Gly Arg Thr Pro Tyr Glu Trp Leu Ser Pro Asp Ser Asp Lys Trp 
625                 630                 635                 640 


Ala Asn Tyr Val Asn Arg Leu Asn Lys Ile Lys Lys Gln Leu Gly Lys 
                645                 650                 655     


Lys Lys Phe Glu Leu Leu Thr Ser Lys Pro Glu Asp Cys Glu Lys Leu 
            660                 665                 670         


Ile Glu Ser Tyr Asn Gly Leu Ala Glu Thr Ser His Ile Ala Arg Val 
        675                 680                 685             


Ala Gln Gln Met Thr Ala Phe Ile Phe Gly Trp Gly Leu Gln Ile Gln 
    690                 695                 700                 


Gly Glu Ser Arg Arg Ile Phe Val Asn Asn Gly Ser Ser Thr Cys Ala 
705                 710                 715                 720 


Ile Arg Lys Arg Tyr Gly Leu Asn Lys Leu Leu Gly Asp Asn Leu Lys 
                725                 730                 735     


Lys Asn Arg Ser Asn Asp Lys His His Ala Leu Asp Ala Ile Cys Ile 
            740                 745                 750         


Ser Phe Ser Arg Asp Phe Lys Tyr Asp Lys Glu Ile Lys Lys Asp Ile 
        755                 760                 765             


Ile Lys Gly Phe Asn Pro Glu Ile Val Lys Asn Ala Ile Asp Lys Ile 
    770                 775                 780                 


Met Pro Tyr Pro Tyr Ala Asn Asp Lys Pro Phe Lys Gly Asn Thr Lys 
785                 790                 795                 800 


Pro Leu Glu Thr Ile Tyr Gly Leu Arg Thr Tyr Gly Asp Lys Ser Tyr 
                805                 810                 815     


Ile Thr Gln Arg Val Glu Leu Asn Ser Ile Asp Lys Lys Ala Thr Lys 
            820                 825                 830         


Ile Lys Ser Ile Ile Asp Glu Thr Ile Lys Asn Asp Leu Leu Asn Lys 
        835                 840                 845             


Leu Lys Glu Asn Pro Thr Glu Gln Glu Trp Lys Leu Met Leu Gln Asn 
    850                 855                 860                 


Tyr Ile His Pro Lys Lys Gln Thr Lys Val Lys Lys Val Met Ile Ser 
865                 870                 875                 880 


Val Ser Glu Gly Glu Ile Thr Lys Asp Ser Asn Asn Arg Glu Arg Met 
                885                 890                 895     


Gly Glu Phe Val Asp Phe Gly Thr Lys Gly Thr Gln His Gln Phe Lys 
            900                 905                 910         


His Ser Lys Arg His Lys Gly Gln Ile Leu Tyr Phe Asn Glu Lys Gly 
        915                 920                 925             


Val Val Glu Val Met Pro Val Tyr Ser Asn Ile Lys Thr Thr Asp Val 
    930                 935                 940                 


Lys Asp Lys Leu Gln Asn Met Gly Cys Lys Leu Tyr Asn Lys Gly Gln 
945                 950                 955                 960 


Met Phe Tyr Ser Gly Cys Leu Val Asp Ile Pro Lys Pro Phe Lys Ala 
                965                 970                 975     


Gly Ser Lys Glu Tyr Pro Ala Gly Arg Tyr Gln Ile Lys Thr Ile Arg 
            980                 985                 990         


Ser Asp Lys Val Ala Glu Leu Glu  Asp Ala Cys Gly Asn  Lys Ile Ser 
        995                 1000                 1005             


Thr Asn  Val Lys Tyr Leu Val  Pro Ala Glu Phe Lys  Lys Val Glu 
    1010                 1015                 1020             


Ser Lys  
    1025 


<210> 71
<211> 1059
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG1-6 (D13A) nickase

<400> 71
Met Glu Lys Glu Lys Asn Asn His Val Trp Gly Phe Ala Ile Gly Lys 
1               5                   10                  15      


Gly Ser Leu Gly Glu Ala Val Arg Val Gly Gly Asp Phe Lys His Ile 
            20                  25                  30          


Ala Ser Val Leu Leu Asp Pro Glu Phe Gly Glu Ile Lys Thr Ala Ala 
        35                  40                  45              


Leu Ala Arg Arg Gln Met Arg Thr Arg Lys Ala His Lys Ala Arg Glu 
    50                  55                  60                  


Lys Trp Leu Glu Glu Cys Leu Asp Gly Thr Gly Val Glu Ile Leu Lys 
65                  70                  75                  80  


Arg Arg Glu Val Gly Ile Val Asp Gly Gln Trp Arg Leu Ile Ser Lys 
                85                  90                  95      


Gly Asp Glu Arg Leu Glu Arg Glu Phe Pro Pro Asn Gly Glu Glu Val 
            100                 105                 110         


Cys Tyr Asn Ser Ile Ala Leu Arg Cys Lys Leu Leu Leu Gly Glu Lys 
        115                 120                 125             


Leu Glu Gly Trp Gln Val Phe Lys Ala Leu Asn Ser Ala Ile Gln Lys 
    130                 135                 140                 


Arg Gly Tyr Asp Glu Asn Ile Pro Trp Gly Glu Ala Glu Glu Lys Ser 
145                 150                 155                 160 


Ser Lys Lys Asp Glu Asp Asp Tyr Ala Gln Lys Leu Ser Gln Tyr Glu 
                165                 170                 175     


Arg Glu Lys Ser Glu Leu Phe Glu Ser Phe Ala Asp Gly Glu Lys Tyr 
            180                 185                 190         


Asp Phe Pro Cys Phe Phe Lys Ala Tyr Lys Met Gly Leu Trp Ser Pro 
        195                 200                 205             


Glu Asn Pro Arg Arg Val Glu Val Arg Ile Asp Cys Arg Ala Gln Lys 
    210                 215                 220                 


Ala Lys Ser Tyr Val Val Ala Arg Lys Tyr Val Glu Arg Glu Phe Glu 
225                 230                 235                 240 


Cys Leu Val Glu Ala Ala Ala Lys Phe Phe Pro Lys Leu Lys Gly Arg 
                245                 250                 255     


Ala Lys Phe Ile Leu Tyr Gly Val Ser Glu Thr Pro Tyr Ala Ser Tyr 
            260                 265                 270         


Tyr Gly Asn Met Arg Lys Lys Phe Gly Leu Lys Arg Gly Ala Glu Ser 
        275                 280                 285             


Asp Trp Thr Ala Leu Gly Gln Lys Val Pro Arg Phe Asp Asn Arg Ile 
    290                 295                 300                 


Ile Asp Lys Cys Arg Leu Ile Pro Arg Met Asn Val Cys Lys Ile Arg 
305                 310                 315                 320 


Pro Leu Asn Glu Ala Arg Asn Glu Lys Asp Leu Leu Tyr Tyr Glu Val 
                325                 330                 335     


Thr Leu Ala Leu Lys Leu Leu Asn Leu Arg Phe Phe Arg Asn Ser Asn 
            340                 345                 350         


Ile Glu Gln Leu Thr Phe Glu Glu Phe Lys Lys Ala Phe Glu Ile Ala 
        355                 360                 365             


Ser Gly Ala Lys Tyr Lys Met Thr Lys Thr Ala Met Lys Lys Phe Leu 
    370                 375                 380                 


Lys Ser Ile Ser Ala Thr Ala Leu Gly Asp Asp Tyr Ala Glu Ile Glu 
385                 390                 395                 400 


Pro Pro Lys Glu Ser Gly Arg Ala Ser Phe Ser Arg Pro Ala Met Asp 
                405                 410                 415     


Ile Leu Lys Glu Leu Ile Phe Ser Gly Met Pro Pro Arg Glu Phe Tyr 
            420                 425                 430         


Gly Arg Lys Ile Pro Glu Ile Ser Asn Thr Asp Lys Asn Lys Gly Val 
        435                 440                 445             


Val Ala Ser Asp Leu Asp Phe Ile Lys Leu Met Gly Asp Cys Pro Trp 
    450                 455                 460                 


Gly Gly Ile Phe Ile Pro Asp Val Gln Thr Tyr Gln Phe Ala Asn Ala 
465                 470                 475                 480 


Ala Phe Pro Asn Pro Asp Ala Gln Ile Asn Lys Leu Ile Gly Ser Gln 
                485                 490                 495     


Asn Asn Pro Ile Val Arg His Arg Leu Thr Phe Phe Phe Glu Arg Ile 
            500                 505                 510         


Lys Phe Leu Glu Ser Lys Tyr Gly Val Pro Asp Lys Val Val Leu Glu 
        515                 520                 525             


Phe Val Arg Asp Asp Phe Met Gly Glu Lys Glu Lys Lys Glu Met Arg 
    530                 535                 540                 


Ile Ala Met Lys Lys Arg Ala Glu Glu Lys Met Met Ile Ala Lys Asp 
545                 550                 555                 560 


Leu Asp Glu His Gly Phe Lys Gly Gly Asn Leu Leu Leu Lys Met Glu 
                565                 570                 575     


Leu Tyr Arg Lys Gln Asn Gly Ile Cys Leu Tyr Thr Gly Asp Ala Leu 
            580                 585                 590         


Pro Leu Ser Glu Leu Ala Thr Leu Glu Ile Glu His Ile Val Pro Arg 
        595                 600                 605             


Ser Arg Gly Gly Pro Asp Ala Gln Tyr Asn Tyr Val Ile Thr Arg Glu 
    610                 615                 620                 


Lys Thr Asn Lys Leu Lys Gly Asp Lys Thr Pro Tyr Glu Trp Leu Ser 
625                 630                 635                 640 


Ser Asp Ala Lys Glu Trp Ala Ser Tyr Thr Asp Arg Val Asn Ala Cys 
                645                 650                 655     


Ala Lys Ala Leu Gly Lys Lys Arg Cys Arg Leu Leu Leu Glu Lys Asp 
            660                 665                 670         


Ala Glu Glu Leu Val Glu Lys Tyr Thr Ala Leu Ala Glu Thr Ala His 
        675                 680                 685             


Ile Ala Lys Leu Ala Gln Lys Ile Val Cys Met His Phe Gly Phe Gln 
    690                 695                 700                 


Phe Gly Gly Leu Ser Gly Thr Lys Arg Val Phe Thr Val Pro Gly Ala 
705                 710                 715                 720 


Leu Thr Ser Arg Ile Arg Lys Phe Tyr Gly Leu Asp Arg Ile Leu His 
                725                 730                 735     


Asn Ala Ala Ala Glu Ser Glu Asn Leu Ser Phe Glu Glu Phe Leu Lys 
            740                 745                 750         


Phe Ser Glu Asp Leu Glu Lys Lys Asn Arg Lys Asn Lys Lys His His 
        755                 760                 765             


Ala Leu Asp Ala Met Cys Leu Cys Phe Ala Pro Thr Gly Val Asp Ser 
    770                 775                 780                 


Arg Arg Ala Lys Leu Gly Glu Ile Leu Pro Glu Lys Leu Arg Ser Glu 
785                 790                 795                 800 


Lys Ala Ala Arg Glu Phe Phe Lys Ser Tyr Leu Asp Lys Ile Met Pro 
                805                 810                 815     


Val Asp Val Ala Pro Lys Lys Pro Arg Leu Glu Asp Gly Ile Tyr Ser 
            820                 825                 830         


Lys Arg Ile Ile Gly Gly Lys Ala Cys Met Val Lys Arg Asn Asn Leu 
        835                 840                 845             


Val Asp Leu Ala Tyr Lys Ser Gly Leu Lys Pro Val Phe Asp Ile Pro 
    850                 855                 860                 


Thr Leu Ile Lys Leu Val Asp Lys Lys Glu Lys Gly Ile Ile Asn Pro 
865                 870                 875                 880 


Gln Ile Arg Lys Met Ile Gly Glu Phe Ala Ala Thr Asn Pro Asp Glu 
                885                 890                 895     


Ser Ala Trp Arg Lys Trp Cys Glu Glu Val Arg Leu Pro Ser Lys Ser 
            900                 905                 910         


Gly Leu Gly Ala Arg Val Leu Arg Val Leu Val Tyr Tyr Gly Glu Ala 
        915                 920                 925             


Asp Glu Tyr Lys Asp Leu Ser Lys Asp Gly Cys Gly Ala Tyr Arg Lys 
    930                 935                 940                 


Gly Asp Gly His Lys Gly Gln Val Val Trp Glu Ser Val Asp Gly Lys 
945                 950                 955                 960 


Tyr Tyr Val Glu Pro Val Tyr Val His Ala Ser Lys Ala Gly Val Met 
                965                 970                 975     


Ala Ala Leu Asn Ala Asn Pro Lys Lys Lys Arg Ile Cys Gly Met Phe 
            980                 985                 990         


Asn Ser His Cys Thr Val Asp Val  Gly Asp Val Tyr Asn  Asp Arg Gly 
        995                 1000                 1005             


Asp Phe  Ile Leu Pro Ala Gly  Arg Tyr Met Val Asn  Thr Ile Leu 
    1010                 1015                 1020             


Thr Thr  Gly Arg Cys Val Leu  Thr Asn Ala Asp Gly  Glu Lys Arg 
    1025                 1030                 1035             


Asn Pro  Ile Asn Ile Asn Tyr  Leu Met Arg Ala Gly  Met Arg Arg 
    1040                 1045                 1050             


Val Glu  Leu Ser Glu Leu 
    1055                 


<210> 72
<211> 1134
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG3-6 (D13A) nickase

<400> 72
Met Ser Thr Asp Met Lys Asn Tyr Arg Ile Gly Val Ala Val Gly Asp 
1               5                   10                  15      


Arg Ser Val Gly Leu Ala Ala Ile Glu Phe Asp Asp Asp Gly Leu Pro 
            20                  25                  30          


Ile Gln Lys Leu Ala Leu Val Thr Phe Arg His Asp Gly Gly Leu Asp 
        35                  40                  45              


Pro Thr Lys Asn Lys Thr Pro Met Ser Arg Lys Glu Thr Arg Gly Ile 
    50                  55                  60                  


Ala Arg Arg Thr Met Arg Met Asn Arg Glu Arg Lys Arg Arg Leu Arg 
65                  70                  75                  80  


Asn Leu Asp Asn Val Leu Glu Asn Leu Gly Tyr Ser Val Pro Glu Gly 
                85                  90                  95      


Pro Glu Pro Glu Thr Tyr Glu Ala Trp Thr Ser Arg Ala Leu Leu Ala 
            100                 105                 110         


Ser Ile Lys Leu Ala Ser Ala Asp Glu Leu Asn Glu His Leu Val Arg 
        115                 120                 125             


Ala Val Arg His Met Ala Arg His Arg Gly Trp Ala Asn Pro Trp Trp 
    130                 135                 140                 


Ser Leu Asp Gln Leu Glu Lys Ala Ser Gln Glu Pro Ser Glu Thr Phe 
145                 150                 155                 160 


Glu Ile Ile Leu Ala Arg Ala Arg Glu Leu Phe Gly Glu Lys Val Pro 
                165                 170                 175     


Ala Asn Pro Thr Leu Gly Met Leu Gly Ala Leu Ala Ala Asn Asn Glu 
            180                 185                 190         


Val Leu Leu Arg Pro Arg Asp Glu Lys Lys Arg Lys Thr Gly Tyr Val 
        195                 200                 205             


Arg Gly Thr Pro Leu Met Phe Ala Gln Val Arg Gln Gly Asp Gln Leu 
    210                 215                 220                 


Ala Glu Leu Arg Arg Ile Cys Glu Val Gln Gly Ile Glu Asp Gln Tyr 
225                 230                 235                 240 


Glu Ala Leu Arg Leu Gly Val Phe Asp His Lys His Pro Tyr Val Pro 
                245                 250                 255     


Lys Glu Arg Val Gly Lys Asp Pro Leu Asn Pro Ser Thr Asn Arg Thr 
            260                 265                 270         


Ile Arg Ala Ser Leu Glu Phe Gln Glu Phe Arg Ile Leu Asp Ser Val 
        275                 280                 285             


Ala Asn Leu Arg Val Arg Ile Gly Ser Arg Ala Lys Arg Glu Leu Thr 
    290                 295                 300                 


Glu Ala Glu Tyr Asp Ala Ala Val Glu Phe Leu Met Asp Tyr Ala Asp 
305                 310                 315                 320 


Lys Glu Gln Pro Ser Trp Ala Asp Val Ala Glu Lys Ile Gly Val Pro 
                325                 330                 335     


Gly Asn Arg Leu Val Ala Pro Val Leu Glu Asp Val Gln Gln Lys Thr 
            340                 345                 350         


Ala Pro Tyr Asp Arg Ser Ser Ala Ala Phe Glu Lys Ala Met Gly Lys 
        355                 360                 365             


Lys Thr Glu Ala Arg Gln Trp Trp Glu Ser Thr Asp Asp Asp Gln Leu 
    370                 375                 380                 


Arg Ser Leu Leu Ile Ala Phe Leu Val Asp Ala Thr Asn Asp Thr Glu 
385                 390                 395                 400 


Glu Ala Ala Ala Glu Ala Gly Leu Ser Glu Leu Tyr Lys Ser Trp Pro 
                405                 410                 415     


Ala Glu Glu Arg Glu Ala Leu Ser Asn Ile Asp Phe Glu Lys Gly Arg 
            420                 425                 430         


Val Ala Tyr Ser Gln Glu Thr Leu Ser Lys Leu Ser Glu Tyr Met His 
        435                 440                 445             


Glu Tyr Arg Val Gly Leu His Glu Ala Arg Lys Ala Val Phe Gly Val 
    450                 455                 460                 


Asp Asp Thr Trp Arg Pro Pro Leu Asp Lys Leu Glu Glu Pro Thr Gly 
465                 470                 475                 480 


Gln Pro Ala Val Asp Arg Val Leu Thr Ile Leu Arg Arg Phe Val Leu 
                485                 490                 495     


Asp Cys Glu Arg Gln Trp Gly Arg Pro Arg Ala Ile Thr Val Glu His 
            500                 505                 510         


Thr Arg Thr Gly Leu Met Gly Pro Thr Gln Arg Gln Lys Ile Leu Asn 
        515                 520                 525             


Glu Gln Lys Lys Asn Arg Ala Asp Asn Glu Arg Ile Arg Asp Glu Leu 
    530                 535                 540                 


Arg Glu Ser Gly Val Asp Asn Pro Ser Arg Ala Glu Val Arg Arg His 
545                 550                 555                 560 


Leu Ile Val Gln Glu Gln Glu Cys Gln Cys Leu Tyr Cys Gly Thr Met 
                565                 570                 575     


Ile Thr Thr Thr Thr Ser Glu Leu Asp His Ile Val Pro Arg Ala Gly 
            580                 585                 590         


Gly Gly Ser Ser Arg Arg Glu Asn Leu Ala Ala Val Cys Arg Ala Cys 
        595                 600                 605             


Asn Ala Lys Lys Lys Arg Glu Leu Phe Tyr Ala Trp Ala Gly Pro Val 
    610                 615                 620                 


Lys Ser Gln Glu Thr Ile Glu Arg Val Arg Gln Leu Lys Ala Phe Lys 
625                 630                 635                 640 


Asp Ser Lys Lys Ala Lys Met Phe Lys Asn Gln Ile Arg Arg Leu Asn 
                645                 650                 655     


Gln Thr Glu Ala Asp Glu Pro Ile Asp Glu Arg Ser Leu Ala Ser Thr 
            660                 665                 670         


Ser Tyr Ala Ala Val Ala Val Arg Glu Arg Leu Glu Gln His Phe Asn 
        675                 680                 685             


Glu Gly Leu Ala Leu Asp Asp Lys Ser Arg Val Val Leu Asp Val Tyr 
    690                 695                 700                 


Ala Gly Ala Val Thr Arg Glu Ser Arg Arg Ala Gly Gly Ile Asp Glu 
705                 710                 715                 720 


Arg Ile Leu Leu Arg Gly Glu Arg Asp Lys Asn Arg Phe Asp Val Arg 
                725                 730                 735     


His His Ala Val Asp Ala Ala Val Met Thr Leu Leu Asn Arg Ser Val 
            740                 745                 750         


Ala Leu Thr Leu Glu Gln Arg Ser Gln Leu Arg Arg Thr Phe Tyr Glu 
        755                 760                 765             


Gln Gly Leu Asp Lys Leu Asp Arg Asn Gln Leu Lys Pro Glu Glu Asp 
    770                 775                 780                 


Trp Arg Asp Phe Thr Gly Leu Ala Pro Ala Ser Gln Glu Lys Phe Leu 
785                 790                 795                 800 


Glu Trp Arg Lys Ala Ala Thr Ile Leu Gly Asp Leu Leu Ala Glu Ala 
                805                 810                 815     


Ile Glu Asp Asp Ser Ile Ala Val Val Ser Pro Leu Arg Leu Arg Pro 
            820                 825                 830         


Gln Asn Gly Ser Val His Leu Glu Thr Ile Ser Ala Val Lys Lys Gln 
        835                 840                 845             


Thr Leu Gly Ser Asp Trp Pro Ala Asp Ala Val Lys Arg Ile Val Asp 
    850                 855                 860                 


Pro Glu Ile Tyr Leu Ala Met Lys Asp Ala Leu Gly Lys Leu Lys Glu 
865                 870                 875                 880 


Leu Pro Glu Asp Ser Ala Arg Ser Leu Glu Leu Pro Asp Gly Arg Phe 
                885                 890                 895     


Val Glu Ala Asp Asp Glu Val Leu Phe Phe Pro Glu Asn Ala Ala Ser 
            900                 905                 910         


Ile Leu Thr Pro Arg Gly Val Ala Glu Ile Gly Gly Ser Ile His His 
        915                 920                 925             


Ala Arg Leu Tyr Gly Trp Leu Thr Lys Lys Gly Glu Leu Lys Val Gly 
    930                 935                 940                 


Met Leu Arg Val Tyr Gly Ala Glu Phe Pro Trp Leu Met Arg Glu Ser 
945                 950                 955                 960 


Gly Ser Arg Asn Val Leu Ser Met Pro Ile His Arg Gly Ser Gln Ser 
                965                 970                 975     


Phe Arg Asp Met Gln Asp Thr Thr Arg Lys Ala Val Glu Ser Gly Glu 
            980                 985                 990         


Ala Val Glu Phe Ala Trp Ile Thr  Gln Asn Asp Glu Leu  Glu Phe Asp 
        995                 1000                 1005             


Pro Asp  Asp Tyr Ile Ala His  Gly Gly Lys Asp Glu  Leu Arg Gln 
    1010                 1015                 1020             


Phe Leu  Gly Phe Met Pro Glu  Cys Arg Trp Arg Val  Asp Gly Phe 
    1025                 1030                 1035             


Lys Lys  Asn Tyr Gln Ile Arg  Ile Arg Pro Ala Met  Leu Ser Arg 
    1040                 1045                 1050             


Glu Gln  Leu Pro Ser Asp Ile  Gln Arg Arg Leu Glu  Ser Lys Thr 
    1055                 1060                 1065             


Leu Thr  Lys Asn Glu Ser Leu  Leu Leu Lys Ala Leu  Asp Thr Gly 
    1070                 1075                 1080             


Leu Val  Val Ala Ile Gly Gly  Leu Leu Pro Leu Glu  Thr Leu Lys 
    1085                 1090                 1095             


Val Ile  Arg Arg Asn Asn Leu  Gly Phe Pro Arg Trp  Arg Gly Asn 
    1100                 1105                 1110             


Gly Asn  Leu Pro Thr Ser Phe  Glu Val Arg Ser Ser  Ala Leu Arg 
    1115                 1120                 1125             


Ala Leu  Gly Val Glu Gly 
    1130                 


<210> 73
<211> 1131
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG3-7 (D12A) nickase

<400> 73
Met Ser Glu His Thr Lys Tyr Arg Ile Gly Ile Ala Val Gly Asp Arg 
1               5                   10                  15      


Ser Val Gly Leu Ala Ala Ile Glu Phe Asp Asp Asp Gly Phe Pro Leu 
            20                  25                  30          


Ser Asn Leu Ala Met Val Thr Tyr Arg His Asp Gly Gly Leu Asp Pro 
        35                  40                  45              


Thr Thr Asn Lys Ser Pro Lys Ser Arg Lys Glu Thr Arg Gly Val Ala 
    50                  55                  60                  


Leu Arg Thr Met Arg Met Arg Arg Arg Lys Lys Ser Arg Leu Lys Asp 
65                  70                  75                  80  


Leu Asp Lys Thr Leu Arg Ala Leu Gly Tyr Val Val Pro Ala Cys Glu 
                85                  90                  95      


Glu Pro Gln Thr Tyr Glu Ala Trp Lys Ser Arg Ala Leu Leu Ala Thr 
            100                 105                 110         


Thr Lys Val Glu Gly Ala Gln Glu Leu Gly Glu His Leu Val Arg Ala 
        115                 120                 125             


Val Arg His Met Ala Arg His Arg Gly Trp Arg Asn Pro Trp Trp Thr 
    130                 135                 140                 


Phe Asn Gln Leu Glu Ile Ala Ser Thr Ala Pro Ser Asp Thr Phe Ala 
145                 150                 155                 160 


Lys Ile Arg Glu Arg Ala Glu Lys Glu Trp Pro Gly Val Val Pro Glu 
                165                 170                 175     


Glu Ala Thr Leu Gly Met Ile Gly Ala Leu Ala Ala Ser Asn Asn Val 
            180                 185                 190         


Leu Leu Arg Pro Arg Thr Tyr Arg Lys Asp Gln Pro Arg His Ser Lys 
        195                 200                 205             


Lys Leu Asn Val Leu Gly Gln Glu Pro Ile Leu Val Asp Lys Val Arg 
    210                 215                 220                 


Gln Glu Asp Leu Leu Ala Glu Leu Arg Thr Ile Cys Lys Lys Gln Asp 
225                 230                 235                 240 


Ile Glu Asp Gln Tyr Glu Lys Leu Ala Gln Ala Val Phe Glu Gln Thr 
                245                 250                 255     


Arg Pro Tyr Val Pro Leu Asp Arg Val Gly Lys Asp Pro Leu Gln Pro 
            260                 265                 270         


Asn Lys Ser Arg Ala Ser Arg Ala Ser Leu Glu Phe Gln Arg Phe Arg 
        275                 280                 285             


Ile Leu Asp Ala Val Ala His Leu Arg Val Arg Val Gly Ala Ser Asp 
    290                 295                 300                 


Arg Arg Ala Leu Thr Asp Glu Glu His Asp Lys Ala Val His Phe Leu 
305                 310                 315                 320 


Met Gly Tyr Ser Lys Lys Asp Val Pro Thr Trp Gly Asp Val Ala Asp 
                325                 330                 335     


Glu Ile Gly Val Glu Pro Ala His Leu Ile Ala Pro Val Ile Asp Asp 
            340                 345                 350         


Val Arg Leu Asn Lys Ala Pro Tyr Asp Arg Ser Ser Thr Glu Phe Glu 
        355                 360                 365             


Gln Lys Met Lys Lys Lys Thr Arg Ala Arg Gln Trp Trp Asp Glu Ala 
    370                 375                 380                 


Asn Leu Glu Ser Arg Ser Gln Leu Ile Leu Leu Val Ser Asp Pro Thr 
385                 390                 395                 400 


Asp Glu Thr Met Arg Leu Ala Glu Val Ser Gly Leu Tyr Glu Val Phe 
                405                 410                 415     


Glu Ser Trp Pro Asp Glu Glu Lys Glu Ala Leu Glu Gly Leu Lys Val 
            420                 425                 430         


Glu Ser Gly Arg Ala Ala Tyr Ser Thr Asp Thr Leu Asn Lys Leu Ser 
        435                 440                 445             


Asp His Met Arg Leu Lys Arg Ile Asp Leu His Glu Ala Arg Lys Ile 
    450                 455                 460                 


Ala Phe Gly Val Asp Asp Met Trp His Pro Pro Leu Asp Arg Ile Asp 
465                 470                 475                 480 


Glu Pro Thr Gly Gln Pro Thr Val Asp Arg Val Leu Thr Ile Val Arg 
                485                 490                 495     


Arg Phe Ile Phe Asp Cys Glu Arg Ala Trp Gly Cys Pro Glu Asn Val 
            500                 505                 510         


Val Ile Glu His Ala Arg Thr Gly Leu Met Gly Pro Ala Gln Arg Ala 
        515                 520                 525             


Asp Val Leu Asn Glu Ile Ala Lys Asn Arg Lys Lys Asn Asp Lys Ile 
    530                 535                 540                 


Arg Glu Asp Leu Arg Ala Gly Gly Ile Asn Asn Pro Thr Arg Ala Asp 
545                 550                 555                 560 


Ile Arg Arg Asn Ala Ile Val Gln Asp Gln Glu Ser Gln Cys Leu Tyr 
                565                 570                 575     


Cys Gly Ala Thr Ile Gly Val Ser Tyr Ser Glu Leu Asp His Ile Val 
            580                 585                 590         


Pro Arg Ala Gly Gly Gly Ser Ser Arg Arg Glu Asn Leu Ala Ala Val 
        595                 600                 605             


Cys Arg Ala Cys Asn Asp Ser Lys Lys Asp Thr Pro Phe Ala Val Trp 
    610                 615                 620                 


Ala Lys Asn Thr Ser Arg Pro Val Thr Leu Glu Gly Thr Ile Gln Arg 
625                 630                 635                 640 


Leu Arg Glu Leu Lys Ala Phe Lys Arg Pro Lys Asp Arg Arg Met Val 
                645                 650                 655     


Asp Leu Ile Ile Arg Arg Leu Lys Gln Thr Glu Lys Asp Asp Pro Ile 
            660                 665                 670         


Asp Glu Arg Ser Leu Ala Ser Thr Ser Tyr Ala Ala Thr Ala Ile Arg 
        675                 680                 685             


Glu Arg Ile Glu Gln Tyr Phe Asn Glu Lys Ala Glu Lys Leu Asp Leu 
    690                 695                 700                 


Ala Lys Val His Ile Asp Val Tyr Ser Gly Ser Leu Thr Arg Glu Ser 
705                 710                 715                 720 


Arg Arg Ala Gly Gly Ile Asp Lys Met Val Leu Leu Arg Gly Gln Ser 
                725                 730                 735     


Asp Lys Asn Arg Phe Asp Val Arg His His Ala Ile Asp Ala Ala Val 
            740                 745                 750         


Leu Thr Leu Leu Asn Arg Ser Val Ala Val Thr Leu Glu Gln Arg Arg 
        755                 760                 765             


Leu Ile Lys Gln Gln Arg Glu Tyr Ser Leu Glu Lys Ser Arg Arg Glu 
    770                 775                 780                 


Arg Asp Asn Val Trp Arg Asp Phe Met Gly Leu Gly Pro Ala Ala Gln 
785                 790                 795                 800 


Glu Lys Phe Ala Lys Trp Lys Lys Thr Ala Tyr Val Leu Ala Asp Ile 
                805                 810                 815     


Ile Lys Glu Ala Ile Ser Asn Asp Ala Ile Pro Val Val Ser Pro Leu 
            820                 825                 830         


Arg Leu Arg Pro Gln Asn Gly Ser Val His Leu Asp Thr Val Asp Ala 
        835                 840                 845             


Val Leu Glu Arg Thr Ile Gly Asp Ala Trp Thr Val Asp Gln Val His 
    850                 855                 860                 


Arg Ile Val Asn Pro Gln Ile Tyr Leu Ala Phe Ala Gly Tyr Leu Gly 
865                 870                 875                 880 


Asn Gln Lys Ala Leu Asp Pro Asp Ser Ser Arg Val Leu Ala Leu Asn 
                885                 890                 895     


Asp Gly Arg Lys Leu Thr Ala Glu Asp Val Ile Tyr Val Phe Pro Glu 
            900                 905                 910         


Lys Ala Ala Ser Ile Leu Thr Pro Arg Gly Val Val Lys Ile Gly Glu 
        915                 920                 925             


Ser Val His His Val Arg Leu Tyr Ala Trp Lys Asn Arg Lys Gly Lys 
    930                 935                 940                 


Ala Glu Val Gly Met Leu Arg Val Phe Gly Ala Glu Phe Pro Trp Leu 
945                 950                 955                 960 


Met Arg Glu Ser Gly Val Lys Asp Val Leu Arg Val Pro Ile His Thr 
                965                 970                 975     


Gly Ser Gln Ser Tyr Arg Asp Leu Ser Phe Thr Val Arg Lys Asn Ile 
            980                 985                 990         


Glu Lys Gly Glu Ala Ala Glu Ile  Gly Trp Leu Thr Gln  Asn Glu Glu 
        995                 1000                 1005             


Leu Glu  Phe Asn Pro Glu Ser  Tyr Leu Gln Glu Gly  Gly Lys Asp 
    1010                 1015                 1020             


Lys Leu  Ala Lys Phe Leu Ala  Phe Leu Pro Glu Thr  Arg Trp Arg 
    1025                 1030                 1035             


Val Asp  Gly Phe Pro Met Pro  Asp Lys Leu Arg Ile  Arg Pro Ala 
    1040                 1045                 1050             


Leu Leu  Ser Arg Glu Glu Ile  Pro Glu Gly Val Phe  Arg Thr Glu 
    1055                 1060                 1065             


Glu Gln  Ser Leu Leu Glu Glu  Ala Leu Thr Lys Gly  Leu Ile Ile 
    1070                 1075                 1080             


Ala Thr  Lys Gly Leu Leu Ser  Leu Pro Asp Val Lys  Val Leu Arg 
    1085                 1090                 1095             


Arg Asn  Asn Leu Gly Ile Pro  Arg Trp Arg Gly Gly  Ser Tyr Arg 
    1100                 1105                 1110             


Pro Val  Ser Leu Asp Ile Gln  Arg Ala Ala Leu Ala  Ala Leu Asp 
    1115                 1120                 1125             


Glu Gln  Glu 
    1130     


<210> 74
<211> 1132
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG3-8 (D13A) nickase

<400> 74
Met Ser Thr Asp Met Lys Asn Tyr Arg Ile Gly Val Ala Val Gly Asp 
1               5                   10                  15      


Arg Ser Val Gly Leu Ala Ala Ile Glu Phe Asp Asp Ala Gly Phe Pro 
            20                  25                  30          


Ile Gln Lys Leu Ala Leu Val Thr Phe Arg His Asp Gly Gly Leu Asp 
        35                  40                  45              


Pro Thr Asp Asn Pro Lys Ser Arg Lys Glu Thr Arg Gly Glu Ala Arg 
    50                  55                  60                  


Arg Arg Met Arg Met Thr Arg Arg Arg Lys Gln Arg Leu Arg Asp Leu 
65                  70                  75                  80  


Asp Lys Val Leu Glu Asn Leu Gly Tyr Thr Val Pro Glu Gly Pro Glu 
                85                  90                  95      


Pro Glu Thr Tyr Glu Ala Trp Thr Ser Arg Ala Leu Leu Ala Ser Ile 
            100                 105                 110         


Lys Leu Ala Ser Ala Asp Glu Leu Asn Glu His Leu Val Arg Ala Val 
        115                 120                 125             


Arg His Ile Ala Arg His Arg Gly Trp Val Asn Pro Trp Trp Ser Leu 
    130                 135                 140                 


Asp Gln Leu Glu Arg Ala Ser Gln Glu Pro Ser Glu Thr Phe Glu Ile 
145                 150                 155                 160 


Ile Leu Ala Arg Ala Arg Glu Leu Phe Gly Glu Arg Val Pro Ala Asn 
                165                 170                 175     


Pro Thr Leu Gly Met Leu Gly Ala Leu Ala Ala Asn Asn Glu Val Leu 
            180                 185                 190         


Leu Arg Pro Arg Ala Glu Lys Lys Lys Lys Thr Gly Tyr Val Arg Gly 
        195                 200                 205             


Thr Pro Leu Leu Ala Ala Gln Val Arg Gln Ile Asp Gln Val Ala Glu 
    210                 215                 220                 


Leu Arg Arg Ile Cys Glu Val Gln Gly Ile Glu Glu Gln Tyr Glu Thr 
225                 230                 235                 240 


Leu Arg Asn Ala Ile Phe Ala His Lys Val Ala Tyr Val Pro Thr Glu 
                245                 250                 255     


Arg Val Gly Lys Asp Pro Leu Ala Pro Ser Lys Asn Arg Thr Ile Arg 
            260                 265                 270         


Ala Ser Leu Glu Phe Gln Glu Phe Arg Ile Leu Asp Ser Val Ala Asn 
        275                 280                 285             


Leu Arg Val Arg Thr Asp Ser Arg Ala Lys Arg Glu Leu Thr Glu Gly 
    290                 295                 300                 


Glu Tyr Asp Ala Ala Val Glu Phe Leu Met Gly Tyr Thr Ala Lys Glu 
305                 310                 315                 320 


Gln Pro Ser Trp Ala Asp Val Ala Glu Glu Ile Gly Val Pro Gly Asn 
                325                 330                 335     


Arg Leu Ile Ala Pro Val Leu Glu Asp Val Gln Gln Lys Thr Ala Pro 
            340                 345                 350         


Phe Asp Arg Ser Ser Ala Ala Phe Glu Lys Ala Met Ser Lys Arg Thr 
        355                 360                 365             


Glu Ala Arg Gln Trp Trp Glu Ala Asn Asp Asp Asp Gln Leu Arg Ser 
    370                 375                 380                 


Leu Phe Ile Met Phe Leu Ala Asp Ala Thr Asn Asp Thr Glu Glu Ala 
385                 390                 395                 400 


Ala Ala Val Ala Gly Leu Pro Glu Leu Tyr Met Ser Trp Pro Ala Glu 
                405                 410                 415     


Glu Arg Glu Ala Leu Ser Asn Ile Asp Phe Glu Lys Gly Arg Val Ala 
            420                 425                 430         


Tyr Ser His Glu Thr Leu Ser Lys Leu Ser Glu Tyr Met His Glu His 
        435                 440                 445             


Arg Val Gly Leu His Glu Ala Arg Lys Ala Val Phe Gly Val Asp Asp 
    450                 455                 460                 


Thr Trp Arg Pro Pro Leu Ala Lys Leu Glu Glu Pro Thr Gly Gln Pro 
465                 470                 475                 480 


Thr Val Asp Arg Val Leu Thr Ile Leu Arg Arg Phe Val Leu Asp Cys 
                485                 490                 495     


Glu Arg Gln Trp Gly Arg Pro Arg Ala Ile Thr Val Glu His Ala Arg 
            500                 505                 510         


Ile Gly Leu Val Gly Pro Ala Gln Arg Gln Asn Ile Leu Lys Glu Gln 
        515                 520                 525             


Glu Asp Asn Arg Lys Asn Asn Glu Cys Ile Arg Asp Glu Leu Arg Lys 
    530                 535                 540                 


Ser Gly Val Glu Asn Pro Ser Arg Thr Glu Val Arg Arg His Leu Val 
545                 550                 555                 560 


Val Gln Asp Gln Glu Ser Gln Cys Leu Tyr Cys Gly Ala Val Ile Arg 
                565                 570                 575     


Thr Asp Thr Ser Glu Leu Asp His Ile Val Pro Arg Ala Gly Gly Gly 
            580                 585                 590         


Ser Ser Arg Arg Glu Asn Leu Ala Ala Val Cys Arg Tyr Cys Asn Ser 
        595                 600                 605             


Lys Lys Asp Arg Thr Leu Phe Tyr Asp Trp Ala Gly Ser Val Arg Leu 
    610                 615                 620                 


Gln Glu Thr Ile Asp Arg Val Arg Gln Leu Lys Ala Phe Lys Asp Ser 
625                 630                 635                 640 


Lys Lys Ala Lys Met Phe Lys Asn Gln Ile Arg Arg Leu Arg Gln Thr 
                645                 650                 655     


Glu Ala Asp Glu Pro Ile Asp Glu Arg Ser Leu Ala Ser Thr Ser Tyr 
            660                 665                 670         


Ala Ala Val Ala Val Arg Glu Arg Leu Glu Gln His Phe Asn Glu Gly 
        675                 680                 685             


Leu Ala Pro Asp Asp Lys Asn Arg Val Val Leu Asp Val Tyr Ala Gly 
    690                 695                 700                 


Ser Val Thr Arg Glu Ser Arg Arg Ala Gly Gly Ile Asp Glu Arg Ile 
705                 710                 715                 720 


Leu Leu Arg Gly Glu Arg Asp Lys Asn Arg Phe Asp Val Arg His His 
                725                 730                 735     


Ala Ile Asp Ala Ala Val Met Thr Leu Leu Asn Arg Ser Val Ala Leu 
            740                 745                 750         


Thr Leu Glu Gln Arg Ser Gln Leu Arg Arg Ala Phe Tyr Glu Gln Gly 
        755                 760                 765             


Leu Asp Lys Leu Asp Arg Asp Gln Leu Lys Pro Glu Glu Asp Trp Arg 
    770                 775                 780                 


Asn Phe Ile Gly Leu Ser Leu Ala Ser Gln Glu Lys Phe Leu Glu Trp 
785                 790                 795                 800 


Lys Lys Val Thr Thr Val Leu Gly Asp Leu Leu Ala Glu Ala Ile Glu 
                805                 810                 815     


Asp Asp Ser Ile Ala Val Val Ser Pro Leu Arg Leu Arg Pro Gln Asn 
            820                 825                 830         


Gly Arg Val His Lys Asp Thr Ile Ala Ala Val Lys Lys Gln Thr Leu 
        835                 840                 845             


Gly Ser Ala Trp Ser Ala Asp Ala Val Lys Arg Ile Val Asp Pro Glu 
    850                 855                 860                 


Ile Tyr Leu Ala Met Lys Asp Ala Leu Gly Lys Ser Lys Val Leu Pro 
865                 870                 875                 880 


Glu Asp Ser Ala Arg Thr Leu Glu Leu Ser Asp Gly Arg Tyr Leu Glu 
                885                 890                 895     


Ala Asp Asp Glu Val Leu Phe Phe Pro Lys Asn Ala Ala Ser Ile Leu 
            900                 905                 910         


Thr Pro Arg Gly Val Ala Glu Ile Gly Gly Ser Ile His His Ala Arg 
        915                 920                 925             


Leu Tyr Ser Trp Leu Thr Lys Lys Gly Glu Leu Lys Ile Gly Met Leu 
    930                 935                 940                 


Arg Val Tyr Gly Ala Glu Phe Pro Trp Leu Met Arg Glu Ser Gly Ser 
945                 950                 955                 960 


His Asp Val Leu Arg Met Pro Ile His Pro Gly Ser Gln Ser Phe Arg 
                965                 970                 975     


Asp Met Gln Asp Thr Thr Arg Lys Ala Val Glu Ser Ser Glu Ala Val 
            980                 985                 990         


Glu Phe Ala Trp Ile Thr Gln Asn  Asp Glu Leu Glu Phe  Glu Pro Glu 
        995                 1000                 1005             


Asp Tyr  Ile Ala His Gly Gly  Lys Asp Glu Leu Arg  Gln Phe Leu 
    1010                 1015                 1020             


Glu Phe  Met Pro Glu Cys Arg  Trp Arg Val Asp Gly  Phe Lys Lys 
    1025                 1030                 1035             


Asn Tyr  Gln Ile Arg Ile Arg  Pro Ala Met Leu Ser  Arg Glu Gln 
    1040                 1045                 1050             


Leu Pro  Ser Asp Ile Gln Arg  Arg Leu Glu Ser Lys  Thr Leu Thr 
    1055                 1060                 1065             


Glu Asn  Glu Ser Leu Leu Leu  Lys Ala Leu Asp Thr  Gly Leu Val 
    1070                 1075                 1080             


Val Ala  Ile Gly Gly Leu Leu  Pro Leu Gly Thr Leu  Lys Val Ile 
    1085                 1090                 1095             


Arg Arg  Asn Asn Leu Gly Phe  Pro Arg Trp Arg Gly  Asn Gly Asn 
    1100                 1105                 1110             


Leu Pro  Thr Ser Phe Glu Val  Arg Ser Ser Ala Leu  Arg Ala Leu 
    1115                 1120                 1125             


Gly Val  Glu Gly 
    1130         


<210> 75
<211> 1055
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG4-5 (D17A) nickase

<400> 75
Met Gly Asp Gln Gln Thr Leu Gln Ala Thr Ser Tyr Cys Leu Gly Leu 
1               5                   10                  15      


Ala Val Gly Val Gln Gly Val Gly Trp Ala Val Ile Asp Leu Asp Asp 
            20                  25                  30          


Gln Gly Gln Pro Leu Gly Val Arg Arg Thr Gly Val Arg Cys Phe Asp 
        35                  40                  45              


Ser Gly Val Gly Ser Glu Thr Glu Phe Glu Lys Gly Val Asp Glu Pro 
    50                  55                  60                  


Pro Ser Thr Lys Arg Arg Gln Ala Arg Gln Gln Arg Arg Gln Leu Trp 
65                  70                  75                  80  


Arg Arg Ala Gln Arg Gln Arg Arg Val Phe Arg Ala Leu Gln Glu Ala 
                85                  90                  95      


Gly Leu Leu Pro Pro Gly Pro Pro Thr Pro Val Ser Arg Asp Lys Ile 
            100                 105                 110         


Leu Arg Thr Leu Asp Ala Asp Leu Ala Ile Gln Phe Gly Ser Leu Glu 
        115                 120                 125             


Asp Arg Ile Ala Ala His Leu Leu Pro Tyr Arg Leu Arg Ala Leu Ala 
    130                 135                 140                 


Leu Asp Thr Pro Leu Pro Pro Tyr Ala Leu Gly Arg Ala Phe Tyr His 
145                 150                 155                 160 


Leu Ala Gln Arg Arg Gly Phe Leu Ser Asn Lys Lys Ala Leu Lys Glu 
                165                 170                 175     


Glu Lys Glu Ala Gly Glu Val Lys Lys Gly Ile Ala Glu Leu Gln Glu 
            180                 185                 190         


Glu Met Asn Arg Ala Gly Ala Arg Ala Leu Gly Glu Tyr Phe Ser Ser 
        195                 200                 205             


Leu Asp Pro Glu Gln Arg Arg Ile Arg Asn Arg Trp Thr Ala Arg Gln 
    210                 215                 220                 


Met Tyr Leu Asp Glu Phe Asp Arg Val Trp Glu Ala Gln Ser Arg Tyr 
225                 230                 235                 240 


His Pro Arg Leu Thr Asp Glu Leu Lys Thr Lys Val Arg Arg Ala Ile 
                245                 250                 255     


Phe Phe Gln Arg Pro Leu Lys Ser Gln Lys Gly Leu Ile Gly Arg Cys 
            260                 265                 270         


Glu Leu Glu Pro Arg His Arg Arg Ala Pro Leu Ala Ser Leu Leu Ala 
        275                 280                 285             


Gln Arg Phe Arg Tyr Trp Gln Lys Ile Asn Asp Leu Glu Val Ile Gln 
    290                 295                 300                 


Pro Asp Gly Val Val Arg Pro Leu Ser Pro Glu Glu Arg His Lys Leu 
305                 310                 315                 320 


Ala His Phe Leu Glu Thr Gln Ala Glu Ala Thr Phe Ala Arg Ile Arg 
                325                 330                 335     


Lys Leu Leu Asp Leu Lys Lys Pro Lys Gly Ser Gln Glu Asp Phe Ala 
            340                 345                 350         


Phe Asn Leu Glu Arg Gly Gly Glu Lys Arg Ile Leu Gly Asn Arg Thr 
        355                 360                 365             


Ala Ala Lys Met Ala Ala Val Leu Gly Gln Arg Trp Ala Ala Met Asp 
    370                 375                 380                 


Asp Glu Asp Lys Arg Arg Leu Val Asn Glu Ile Leu Ser Phe Glu Lys 
385                 390                 395                 400 


Glu Ala Pro Leu Ala Arg Arg Leu Val Arg Ala Trp Asn Leu Asp Glu 
                405                 410                 415     


Pro Thr Ala Ala Gln Leu Ala Ala Leu Asp Phe Glu Pro Gly Tyr Ala 
            420                 425                 430         


Ala Leu Ser Ala Arg Ala Ile Arg Lys Leu Leu Pro Leu Met Gln Arg 
        435                 440                 445             


Glu Gly Met Arg Phe Ala Thr Ala Arg Lys Gln Val Tyr Gly Asp His 
    450                 455                 460                 


Leu Leu Gly Thr Arg Ala Tyr Asp Ala Leu Pro Pro Val Ala Lys Ala 
465                 470                 475                 480 


Ile Pro Ser Leu Arg Asn Pro Ala Val Cys Arg Ala Leu Thr Glu Val 
                485                 490                 495     


Arg Lys Val Val Asn Ala Leu Val Arg Ala Tyr Gly Lys Pro Ala Met 
            500                 505                 510         


Val Arg Ile Glu Leu Ala Arg Asp Met Lys Arg Gly Arg Lys Gln Arg 
        515                 520                 525             


Lys Glu Thr Trp Gln Ala Met Arg Glu Asn Glu Arg Cys Arg Leu Asp 
    530                 535                 540                 


Ala Arg Lys Leu Leu Gln Asp His Gly Ile Gln Glu Pro Arg Pro Ala 
545                 550                 555                 560 


Asp Ile Leu Lys Val Leu Leu Ala Glu Glu Cys Asn Trp Glu Cys Pro 
                565                 570                 575     


Tyr Thr Gly Lys Ala Ile Ser Met Asp Ala Leu Ile Gly Ser Gln Pro 
            580                 585                 590         


Gln Phe Asp Val Glu His Ile Ile Pro Phe Ser Arg Ser Leu Asp Asn 
        595                 600                 605             


Ser Phe Leu Asn Lys Thr Leu Cys Tyr His Glu Glu Asn Arg Asn Val 
    610                 615                 620                 


Lys Gln Asn Arg Thr Pro Phe Glu Ala Tyr Gly Gly Asp Glu Gln Arg 
625                 630                 635                 640 


Trp His Glu Ile Leu Gln Arg Val Arg Arg Phe Arg Gly Ser Ala Ala 
                645                 650                 655     


Glu Ser Lys Leu Arg Lys Phe Gln Leu Arg Gln Leu Pro Ala Asp Phe 
            660                 665                 670         


Thr Asn Arg His Leu Asn Asp Thr Arg Tyr Ala Ser Arg Leu Ala Ala 
        675                 680                 685             


Asp Tyr Leu Ala Leu Leu Tyr Gly Gly Arg Val Asp Ala Asn Gly Arg 
    690                 695                 700                 


Gln Arg Val Gln Val Gly Thr Gly Ala Thr Thr Gly Phe Leu Arg Asp 
705                 710                 715                 720 


Glu Trp Gly Leu Asn Ala Ile Leu Gly Asp Asn Asp Glu Lys Glu Arg 
                725                 730                 735     


Ala Asp His Arg His His Ala Ile Asp Ala Val Val Ile Ala Leu Thr 
            740                 745                 750         


Gly Pro Gly Thr Val Gln Ala Leu Thr Arg Ala Ala Leu Arg Ala Lys 
        755                 760                 765             


Glu Leu Gly Arg Arg Leu Phe Val Pro Leu Asp Pro Pro Trp Ala Asp 
    770                 775                 780                 


Arg Asp Ser Phe Leu Arg Asp Val Arg Ala Ser Val Glu Ala Ile Thr 
785                 790                 795                 800 


Val Ser Tyr Arg Val Asp Arg Lys Val Ser Gly Gln Leu His Glu Glu 
                805                 810                 815     


Ser Asn Tyr Ser Lys Pro His Met Thr Val Asp Asn Lys Gly Asn Leu 
            820                 825                 830         


Val Glu His Arg His Ile Arg Lys Pro Leu Lys Asp Met Ser Val Glu 
        835                 840                 845             


Glu Val Glu Ala Ile Val Asp Asp Arg Val Arg Lys Leu Val Gln Glu 
    850                 855                 860                 


Lys Leu Arg Gln Leu Gly Gln Glu Pro Lys Lys Ala Phe Ala Asp Glu 
865                 870                 875                 880 


Ala Asn His Pro Tyr Phe Thr Thr Ala Asp Gly Arg Leu Val Pro Ile 
                885                 890                 895     


His Lys Ala Arg Ile Arg Lys Thr Val Ala Thr Ile Thr Val Gly Pro 
            900                 905                 910         


Pro Gln Cys Pro Arg His Val Ala Pro Gly Leu Asn His His Ile Glu 
        915                 920                 925             


Ile Leu Ala Val Arg Asp Pro Ala Gly Ala Val Thr His Trp Glu Gly 
    930                 935                 940                 


Glu Leu Val Ser Leu Phe Glu Ala Ala Arg Arg Val Lys Ala Gly Glu 
945                 950                 955                 960 


Pro Val Val Arg Arg Asn His Gly Pro Asn Lys Asp Phe Leu Phe Ser 
                965                 970                 975     


Leu Ala Lys Gly Glu Tyr Val Glu Met Glu Leu Gln Pro Gly Lys Arg 
            980                 985                 990         


Gln Leu Phe Arg Val Thr Val Ile  Ser Ala Lys Gln Ile  Glu Phe Arg 
        995                 1000                 1005             


Leu His  His Asp Ala Arg Pro  Thr Met Leu Leu Arg  Lys Thr Pro 
    1010                 1015                 1020             


Gly Ala  Arg Val Ile Arg Ser  Pro Gly Ser Leu Phe  Lys Ala Lys 
    1025                 1030                 1035             


Ala Arg  Lys Val Ala Val Asp  Pro Leu Gly Asn Val  Phe Pro Ala 
    1040                 1045                 1050             


Asn Asp  
    1055 


<210> 76
<211> 1003
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG14-1 (D23A) nickase

<400> 76
Met Glu Ile Thr Ile Asn Arg Glu Ile Gly Lys Leu Gly Leu Pro Arg 
1               5                   10                  15      


His Leu Val Leu Gly Met Ala Pro Gly Ile Ala Ser Cys Gly Phe Ala 
            20                  25                  30          


Leu Ile Asp Thr Ala Asn His Glu Ile Leu Asp Leu Gly Val Arg Leu 
        35                  40                  45              


Phe Asp Ser Pro Thr His Pro Lys Thr Gly Gln Ser Leu Ala Val Ile 
    50                  55                  60                  


Arg Arg Gly Phe Arg Ser Thr Arg Arg Asn Ile Asp Arg Thr Gln Ala 
65                  70                  75                  80  


Arg Leu Lys His Cys Leu Gln Val Leu Lys Ala Tyr Gly Leu Ile Pro 
                85                  90                  95      


Gln Asp Ala Thr Lys Glu Tyr Phe His Thr Thr Lys Gly Asp Lys Gln 
            100                 105                 110         


Pro Leu Lys Leu Arg Val Asp Gly Leu Asp Arg Leu Leu Asn Asp Arg 
        115                 120                 125             


Glu Trp Ala Leu Val Leu Tyr Ser Leu Cys Lys Arg Arg Gly Tyr Ile 
    130                 135                 140                 


Pro His Gly Glu Gly Asn Gln Asp Lys Ser Ser Glu Gly Gly Lys Val 
145                 150                 155                 160 


Leu Ser Ala Leu Ala Ala Asn Lys Glu Ala Ile Ala Glu Thr Ser Cys 
                165                 170                 175     


Arg Thr Val Gly Glu Trp Leu Ala Gln Gln Pro Gln Ser Arg Asn Arg 
            180                 185                 190         


Gly Gly Asn Tyr Asp Lys Tyr Val Thr His Ala Gln Leu Ile Glu Glu 
        195                 200                 205             


Thr His Ile Leu Phe Asp Ala Gln Arg Ser Phe Gly Ser Lys Tyr Ala 
    210                 215                 220                 


Ser Pro Glu Phe Glu Ala Ala Tyr Ile Glu Val Cys Asp Trp Glu Arg 
225                 230                 235                 240 


Ser Arg Lys Asp Phe Asp Arg Arg Thr Tyr Asp Leu Val Gly His Cys 
                245                 250                 255     


Ser Tyr Phe Pro Thr Glu Lys Arg Ala Ala Arg Cys Thr Leu Thr Ser 
            260                 265                 270         


Glu Leu Val Ser Ala Tyr Gly Ala Leu Gly Asn Ile Thr Ile Ile His 
        275                 280                 285             


Glu Asn Gly Thr Ser Arg Ala Leu Ser Ala Thr Glu Arg Asp Glu Cys 
    290                 295                 300                 


Ile Ala Ile Leu Phe Ser Cys Glu Pro Ile Arg Gly Asn Lys Asp Cys 
305                 310                 315                 320 


Ala Val Lys Phe Gly Ala Leu Arg Lys Ala Leu Asp Leu Ser Ser Gly 
                325                 330                 335     


Asp Tyr Phe Lys Gly Val Pro Ala Ala Asp Glu Lys Thr Arg Glu Val 
            340                 345                 350         


Tyr Lys Pro Lys Gly Trp Arg Val Leu Arg Asn Thr Leu Asn Ala Ala 
        355                 360                 365             


Asn Pro Ile Leu Leu Gln Arg Leu Arg Asp Asp Arg Asp Leu Ala Asp 
    370                 375                 380                 


Ala Val Met Glu Ala Val Ala Tyr Ser Ser Ala Leu Pro Val Leu Gln 
385                 390                 395                 400 


Glu Gln Leu Gln Gly Leu Pro Leu Ser Glu Ala Glu Ile Glu Ala Leu 
                405                 410                 415     


Cys Arg Leu Pro Tyr Ser Ser Lys Ala Leu Asn Gly Tyr Gly Asn Arg 
            420                 425                 430         


Ser Lys Lys Ala Leu Asp Met Leu Leu Asp Cys Leu Glu Glu Pro Glu 
        435                 440                 445             


Val Leu Asn Leu Thr Gln Ala Glu Asn Asp Cys Gly Leu Leu Gly Leu 
    450                 455                 460                 


Arg Ile Ala Gly Ala Gln Leu Glu Arg Ser Asp Arg Leu Met Pro Tyr 
465                 470                 475                 480 


Glu Thr Trp Ile Glu Arg Thr Gly Arg Thr Asn Asn Asn Pro Val Val 
                485                 490                 495     


Ile Arg Ser Met Ser Gln Met Arg Lys Val Val Asn Ala Ile Cys Arg 
            500                 505                 510         


Lys Trp Gly Val Pro Asn Glu Ile His Val Glu Leu Asp Arg Glu Leu 
        515                 520                 525             


Arg Leu Pro Gln Arg Ala Lys Asp Glu Ile Ala Lys Ala Asn Lys Lys 
    530                 535                 540                 


Asn Glu Lys Asn Arg Glu Arg Ile Ala Gly Gln Ile Ala Glu Leu Arg 
545                 550                 555                 560 


Gly Cys Thr Ala Asp Glu Val Thr Gly Lys Gln Ile Glu Lys Tyr Arg 
                565                 570                 575     


Leu Trp Glu Glu Gln Glu Tyr Phe Asp Leu Tyr Thr Gly Ala Lys Ile 
            580                 585                 590         


Glu Val Asp Arg Leu Ile Ser Asp Asp Thr Tyr Thr Gln Ile Asp His 
        595                 600                 605             


Ile Leu Pro Phe Ser Arg Thr Gly Glu Asn Ser Arg Asn Asn Lys Val 
    610                 615                 620                 


Leu Val Leu Ala Lys Ser Asn Gln Asp Lys Arg Glu Gln Thr Pro Tyr 
625                 630                 635                 640 


Glu Trp Met Ser His Asp Gly Ala Pro Ser Trp Asp Ala Phe Glu Arg 
                645                 650                 655     


Arg Val Gln Glu Asn Gln Lys Leu Ser Arg Arg Lys Lys Asn Phe Leu 
            660                 665                 670         


Leu Glu Lys Asp Leu Asp Thr Lys Glu Gly Glu Phe Leu Ala Arg Ser 
        675                 680                 685             


Phe Thr Asp Thr Ala Tyr Met Ser Arg Glu Val Cys Ala Tyr Leu Ala 
    690                 695                 700                 


Asp Cys Leu Leu Phe Pro Asp Asp Gly Ala Lys Ala His Val Val Pro 
705                 710                 715                 720 


Thr Thr Gly Arg Ala Thr Ala Trp Leu Arg Arg Arg Trp Gly Leu Asn 
                725                 730                 735     


Phe Gly Ser Asn Gly Glu Lys Asp Arg Ser Asp Asp Arg His His Ala 
            740                 745                 750         


Thr Asp Ala Cys Val Ile Ala Ala Cys Ser Pro Ser Leu Val Ile Lys 
        755                 760                 765             


Thr Ala Arg Ile Asn Gln Glu Thr His Trp Ser Ile Thr Arg Gly Met 
    770                 775                 780                 


Asn Glu Thr Gln Arg Arg Asp Ala Ile Met Lys Ala Leu Glu Ser Val 
785                 790                 795                 800 


Met Pro Trp Glu Thr Phe Ala Asn Glu Val Arg Ala Ala His Asp Phe 
                805                 810                 815     


Val Val Pro Thr Arg Phe Val Pro Arg Lys Gly Lys Gly Glu Leu Phe 
            820                 825                 830         


Glu Gln Thr Val Tyr Arg Tyr Ala Gly Val Asn Ala Gln Gly Lys Asp 
        835                 840                 845             


Ile Ala Arg Lys Ala Ser Ser Asp Lys Asp Ile Val Met Gly Asn Ala 
    850                 855                 860                 


Val Val Ser Ala Asp Glu Lys Ser Val Ile Lys Val Ser Glu Met Leu 
865                 870                 875                 880 


Cys Leu Arg Leu Trp His Asp Pro Glu Ala Lys Lys Gly Gln Gly Ala 
                885                 890                 895     


Trp Tyr Ala Asp Pro Val Tyr Lys Ala Asp Ile Pro Ala Leu Lys Asp 
            900                 905                 910         


Gly Thr Tyr Val Pro Arg Ile Ala Lys Ala His Thr Gly Arg Lys Ala 
        915                 920                 925             


Trp Lys Pro Val Pro Glu Ser Ala Met Ala Lys Pro Pro Leu Glu Ile 
    930                 935                 940                 


Tyr Phe Gly Asp Leu Val Gln Ile Gly Asp Phe Ile Gly Arg Phe Ser 
945                 950                 955                 960 


Gly Tyr Asn Ile Asn Asn Ala Asn Trp Ser Phe Thr Asp Arg Leu Thr 
                965                 970                 975     


Arg Leu Asn Leu Ser Cys Pro Thr Val Gly Gln Leu Asn Asn Asp Leu 
            980                 985                 990         


Ser Pro Val Val Ile Arg Glu Ser  Pro Ile Lys 
        995                 1000             


<210> 77
<211> 1082
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG15-1 (D8A) nickase

<400> 77
Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu Gly 
            20                  25                  30          


Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly Ala 
        35                  40                  45              


Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val Ile 
    50                  55                  60                  


Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys Lys 
65                  70                  75                  80  


His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val Pro 
                85                  90                  95      


Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu Lys 
            100                 105                 110         


Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys His 
        115                 120                 125             


Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp Glu 
    130                 135                 140                 


Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys Asn 
145                 150                 155                 160 


Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg Lys 
                165                 170                 175     


Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg Ala 
            180                 185                 190         


Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn Leu 
        195                 200                 205             


Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile Leu 
    210                 215                 220                 


Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser Pro 
225                 230                 235                 240 


Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu Glu 
                245                 250                 255     


Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met Ala 
            260                 265                 270         


Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg Gly 
        275                 280                 285             


Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp Leu 
    290                 295                 300                 


Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val Leu 
305                 310                 315                 320 


Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu Lys 
                325                 330                 335     


Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu Lys 
            340                 345                 350         


Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala Leu 
        355                 360                 365             


Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln Leu 
    370                 375                 380                 


Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr Ile 
385                 390                 395                 400 


Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu Ala 
                405                 410                 415     


Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser Leu 
            420                 425                 430         


Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val Tyr 
        435                 440                 445             


Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser Leu 
    450                 455                 460                 


Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile Ser 
465                 470                 475                 480 


Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn Pro 
                485                 490                 495     


Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe Arg 
            500                 505                 510         


Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys Asn 
        515                 520                 525             


Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg Pro 
    530                 535                 540                 


Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn Gly 
545                 550                 555                 560 


Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu Lys 
                565                 570                 575     


Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg Ser 
            580                 585                 590         


Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu Asn 
        595                 600                 605             


Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala Pro 
    610                 615                 620                 


Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg Asn 
625                 630                 635                 640 


Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr Thr 
                645                 650                 655     


Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile Ser 
            660                 665                 670         


Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys Gly 
        675                 680                 685             


Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val Thr 
    690                 695                 700                 


Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn Gly 
705                 710                 715                 720 


Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr Gln 
                725                 730                 735     


Gly Ile Val Asn Lys Val Ser Arg Tyr Ser Lys Ser Arg Glu Leu Trp 
            740                 745                 750         


Asp Tyr Glu Val Asp Met Glu Thr Gly Glu Val Leu Gln Lys Lys Asn 
        755                 760                 765             


Lys Asn Thr Lys Asp Val Phe Pro Glu Pro Trp Leu Asn Phe Arg Tyr 
    770                 775                 780                 


Glu Leu Glu Gln Lys Val Arg Val Arg Pro Leu Asp Ile Pro Glu Thr 
785                 790                 795                 800 


Ala Asp Ile Thr Glu Met Glu Glu Pro Phe Val Ser His Met Pro Asn 
                805                 810                 815     


Arg Lys Ile His Gly Pro Ala His Lys Glu Thr Ile Arg Ser Gly Arg 
            820                 825                 830         


Leu Lys Glu Glu Gly Tyr Thr Ile Ser Lys Thr Ala Leu Ile Asp Leu 
        835                 840                 845             


Lys Leu Thr Glu Asp Lys Glu Glu Ile Lys Gly Tyr Tyr Asn Lys Glu 
    850                 855                 860                 


Ser Asp Arg Leu Leu Tyr Glu Ala Leu Lys Lys Gln Leu Gln Arg Tyr 
865                 870                 875                 880 


Gly Gly Lys Ala Lys Glu Ala Phe Lys Glu Pro Phe His Lys Pro Lys 
                885                 890                 895     


Ala Asp Gly Thr Pro Gly Pro Ile Val Asn Lys Val Lys Ile Met Glu 
            900                 905                 910         


Lys Ser Thr Met Leu Ile Pro Val Asn Gly Gly Lys Gly Leu Ala Ser 
        915                 920                 925             


Asn Gly Asn Met Val Arg Ile Asp Val Phe Arg Ala Glu Glu Lys Gly 
    930                 935                 940                 


Lys Lys Lys Tyr Tyr Phe Ile Pro Val Tyr Val Ala Asp Thr Val Lys 
945                 950                 955                 960 


Glu Glu Leu Pro Asn Arg Ala Val Leu Ala His Lys Pro Tyr Glu Ala 
                965                 970                 975     


Trp Lys Ile Met Lys Glu Glu Asn Phe Ile Phe Ser Leu Tyr Pro Asn 
            980                 985                 990         


Asp Leu Ile Phe Val Asp Ala Gly  Lys Glu Ile Pro Phe  Lys Ala Ala 
        995                 1000                 1005             


Leu Lys  Gly Ser Thr Leu Asp  Pro Glu Lys Lys Ala  Ser Arg Phe 
    1010                 1015                 1020             


Leu Met  Tyr Tyr Lys Gly Ala  Asp Ile Ala Thr Gly  Ser Ile Ser 
    1025                 1030                 1035             


Gly Val  Asn His Asp Glu Thr  Tyr Lys Ala Arg Gly  Val Gly Ile 
    1040                 1045                 1050             


Gln Ser  Leu Arg Glu Ile Lys  Lys Cys Cys Ile Asp  Val Leu Gly 
    1055                 1060                 1065             


Asn Ile  Ser Phe Ala Ser Lys  Glu Lys Arg Gln Thr  Phe Arg 
    1070                 1075                 1080         


<210> 78
<211> 1348
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: nickase

<220>
<223> Description: nMG18-1 (D12A) nickase

<400> 78
Met Asp Gln Ser Lys Glu Tyr Tyr Ile Gly Leu Ala Gly Gly Thr Asp 
1               5                   10                  15      


Ser Val Gly Trp Ala Val Leu Asn Pro Asp Tyr Thr Val Val Lys Lys 
            20                  25                  30          


Arg Gly Lys Ser Leu Trp Gly Val Arg Leu Phe Glu Ser Gly Gln Thr 
        35                  40                  45              


Ala Ala Glu Arg Arg Leu Ala Arg Ser Ala Arg Arg Arg Thr Asp Arg 
    50                  55                  60                  


Lys Lys Trp Arg Leu Lys Leu Leu Lys Glu Leu Phe Ala Glu Glu Val 
65                  70                  75                  80  


Ala Ala Cys Asp Pro Ala Phe Phe Ala Arg Leu Ala Glu Ser Arg Tyr 
                85                  90                  95      


Trp Glu Asp Asp Lys Lys Val Asp Gly Ala Asp Thr Leu Phe His Asp 
            100                 105                 110         


Ala Asp Phe Thr Asp Lys Asp Tyr His Arg Gln Phe Pro Thr Ile Tyr 
        115                 120                 125             


His Leu Arg Arg His Leu Met Cys Cys Thr Glu Lys Pro Asp Ile Arg 
    130                 135                 140                 


Leu Leu Tyr Leu Ala Val Ala His Met Ile Lys Asn Arg Gly His Phe 
145                 150                 155                 160 


Leu Asn Glu Gly Gln Asp Leu Asn Gln Val Arg Arg Phe Asp Asp Val 
                165                 170                 175     


Trp Gln Asn Leu Cys Ala Ala Val Ser Asp Val Leu Gly Ile Glu Leu 
            180                 185                 190         


Ala Val Glu Asn Gly Ala Lys Ala Ala Glu Val Leu Thr Ser Ser Val 
        195                 200                 205             


Lys Ile Lys Glu Lys Thr Lys Gln Leu Glu Ser Val Leu Gly Glu Gly 
    210                 215                 220                 


Glu Ser Glu Ala Asp Arg Ser Val Cys Lys Ala Leu Ala Gly Leu Leu 
225                 230                 235                 240 


Ala Gly Ala Lys Val Ser Ala Val Asp Leu Phe Asn Gly Asp Glu Ala 
                245                 250                 255     


Leu Lys Glu Cys Gly Lys Phe Ser Leu Ala Glu Lys Ser Ile Glu Glu 
            260                 265                 270         


Leu Glu Pro Asp Leu Gly Asp Ala Leu Gly Glu Glu Arg Phe Ser Leu 
        275                 280                 285             


Leu Gln Tyr Ala Tyr Ala Ala Tyr Asn Trp Ala Val Phe Ala Arg Leu 
    290                 295                 300                 


Leu Pro Glu Gly Lys Thr Ile Ser Glu Val Lys Thr Glu Glu Tyr Glu 
305                 310                 315                 320 


Ala Tyr Lys Ala Asp Leu Arg Ala Leu Lys Gln Leu Val Lys Gln Tyr 
                325                 330                 335     


Ala Ser Asp Gln Tyr Tyr Arg Val Phe Arg Ser Ala Lys Ile Lys Gly 
            340                 345                 350         


Asn Tyr Ala Ser Tyr Val Gly Lys Thr Asn Cys Gly Asn Glu Lys Ser 
        355                 360                 365             


Asn Pro Glu Ser Cys Lys Pro Glu Glu Phe Phe Ala Phe Leu Lys Lys 
    370                 375                 380                 


Glu Leu Lys Pro Leu Trp Ser Gln Asn Ile Pro Ala Ala Ala Ser Leu 
385                 390                 395                 400 


Ala Ala Lys Leu Glu Ser Gly Thr Leu Leu Arg Arg Gln Arg Ser Gly 
                405                 410                 415     


Glu Asn Ala Ala Ile Pro Tyr Gln Ala His Leu Gln Glu Leu Gln Ile 
            420                 425                 430         


Ile Leu Lys Asn Met Gln Gln Phe Tyr Pro Phe Leu Gln Lys Ala Asp 
        435                 440                 445             


Glu Asn Gly Phe Thr Pro Ala Tyr Lys Ile Glu Lys Leu Leu Thr Phe 
    450                 455                 460                 


Arg Ile Pro Tyr Tyr Val Gly Pro Leu Asn Asp Ala His Lys Glu Lys 
465                 470                 475                 480 


Gly Phe Cys Trp Ile Val Lys Lys Ala Asp Val Pro Val Leu Pro Trp 
                485                 490                 495     


Asn Phe Asp Gln Val Val Asp Glu Asn Ala Cys Glu Asp Ala Phe Ile 
            500                 505                 510         


Gly Arg Met Ser Ala Thr Cys Thr Tyr Leu Val Gly Glu Asn Val Leu 
        515                 520                 525             


Pro Gln Asn Ser Pro Leu Tyr Cys Arg Phe Met Val Leu Asn Glu Leu 
    530                 535                 540                 


Asn Asn Leu Arg Ile Arg Ser Glu Lys Ile Thr Pro Ala Leu Lys Gln 
545                 550                 555                 560 


Ala Ile Phe Thr Gln Leu Phe Cys Ala Gly Lys Gly Gly Lys Val Thr 
                565                 570                 575     


Gln Lys Lys Leu Leu Asp Phe Leu His Ser Gln Gly Ile Asp Ala Asn 
            580                 585                 590         


Lys Gln Asp Ile Ser Gly Ile Asp Gly Asp Phe Lys Ala Ser Met Ala 
        595                 600                 605             


Pro Ala Val Lys Leu Gln Ser Ile Phe Thr Glu Pro Leu Pro Pro Glu 
    610                 615                 620                 


Ile Leu Glu Arg Leu Ala Phe Ala Leu Thr Val Ser Gly Glu Ser His 
625                 630                 635                 640 


Lys Met Leu Gln Asn Arg Ile Arg Ala Ile Leu Pro Asn Ala Asp Asn 
                645                 650                 655     


Ala Val Ile Arg Lys Val Cys Ala Leu Lys Phe Thr Gly Trp Gly Arg 
            660                 665                 670         


Leu Ser Gln Lys Leu Leu Thr Glu Ile Tyr Asp Val Asp Gln Thr Thr 
        675                 680                 685             


Gly Glu Cys Arg Ser Leu Met Asp Leu Leu Trp Ser Thr Asn Asn Asn 
    690                 695                 700                 


Leu Met Gln Leu Leu Ser Gly Asp Phe Thr Phe Ala Ala Gly Ile Asp 
705                 710                 715                 720 


Ala Tyr Asn Ala Ala Gln Asn Gly Thr Glu Asn Ile Phe Asp Tyr Asp 
                725                 730                 735     


His Leu Val Lys Asp Leu Tyr Val Ser Pro Ser Val Lys Arg Gly Leu 
            740                 745                 750         


Trp Gln Thr Leu Leu Leu Val Lys Glu Val Arg Lys Ile Met Gly Cys 
        755                 760                 765             


Asp Pro Ala Arg Val Phe Ile Glu Met Ala Arg Gly Val Ser Pro Glu 
    770                 775                 780                 


Gln Lys Asn Lys Arg Thr Val Ser Arg Lys Asp Gln Leu Leu Ala Leu 
785                 790                 795                 800 


Tyr Lys Ala Cys Lys Glu Asp Ala Arg Asp Trp Cys Gly Glu Leu Glu 
                805                 810                 815     


Ala Leu Pro Glu Asp Lys Leu Arg Ser Asp Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Thr Gln Met Gly Arg Cys Met Tyr Ser Gly Glu Pro Ile Arg Leu Asp 
        835                 840                 845             


Asp Leu Met His Asp Thr Ala Asn Gln Leu Tyr Asp Ile Asp His Ile 
    850                 855                 860                 


Tyr Pro Gln Ser Lys Thr Lys Asp Asp Ser Leu Thr Asn Arg Val Leu 
865                 870                 875                 880 


Val Lys Arg Gln Leu Asn Ala Ile Lys Ser Asn Asp Tyr Pro Leu Pro 
                885                 890                 895     


Gln Thr Met Arg Gln Thr Ala Leu Trp Lys Ala Leu Leu Glu Lys Gly 
            900                 905                 910         


Leu Ile Ser Lys Gln Lys Tyr Leu Arg Leu Thr Arg Thr Thr Pro Phe 
        915                 920                 925             


Thr Glu Glu Glu Leu Ala Gly Phe Ile Asn Arg Gln Leu Val Glu Thr 
    930                 935                 940                 


Arg Gln Ser Thr Lys Ala Ala Ala His Leu Leu Glu Gln Leu Leu Pro 
945                 950                 955                 960 


Asn Thr Glu Val Val Tyr Val Lys Ala Gly Leu Ala Ala Asp Phe Arg 
                965                 970                 975     


Gln Glu Phe Gly Phe Val Lys Cys Arg Asp Ala Asn Asp Phe His His 
            980                 985                 990         


Ala Lys Asp Ala Tyr Leu Asn Ala  Val Val Gly Asn Val  Tyr His Glu 
        995                 1000                 1005             


Lys Phe  Thr Lys Asn Pro Leu  Arg Phe Val Arg Ser  Gly Gln Glu 
    1010                 1015                 1020             


Tyr Ser  Leu Asn Leu Ser Ala  Leu Phe Gln Asn Trp  Asn Ile Tyr 
    1025                 1030                 1035             


Lys Gly  Gly Arg Val Ile Trp  Gln Lys Gly Glu Asp  Gly Ser Leu 
    1040                 1045                 1050             


Glu Thr  Val Arg Ala Arg Met  Ala Lys Asn Asp Pro  Met Val Thr 
    1055                 1060                 1065             


Arg Tyr  Cys Thr Glu Gly Arg  Gly Ala Leu Tyr Asp  Leu Gln Pro 
    1070                 1075                 1080             


Met Lys  Lys Ser Lys Gly Gln  Leu Pro Leu Lys Ser  Ser Asp Glu 
    1085                 1090                 1095             


Arg Leu  Gln His Ile Asp Arg  Tyr Gly Gly Tyr Asn  Lys Leu Ala 
    1100                 1105                 1110             


Gly Ala  Tyr Phe Thr Leu Ala  Ala Tyr Tyr Lys Lys  Gly Lys Arg 
    1115                 1120                 1125             


Val Lys  Ser Ile Glu Ser Val  Pro Leu Tyr Leu Ala  Ala Lys Leu 
    1130                 1135                 1140             


Gln Arg  Asp Pro Ala Ala Leu  Gln Gln Tyr Leu Ala  Asp Gln Leu 
    1145                 1150                 1155             


Gly Thr  Asp Arg Val Glu Ile  Leu Val Pro Glu Ile  Lys Leu Gly 
    1160                 1165                 1170             


Thr Leu  Phe Lys Trp Asn Gly  Tyr Pro Met Thr Leu  Ser Gly Arg 
    1175                 1180                 1185             


Thr Gly  Pro Gln Leu Leu Phe  Arg Asn Ala Ala Glu  Leu Arg Thr 
    1190                 1195                 1200             


Asn Ala  Glu Gln Glu Gln Tyr  Ile Lys Lys Met Ser  Arg Tyr Leu 
    1205                 1210                 1215             


Glu Lys  Cys Lys Gly Arg Lys  Glu Pro Leu Pro Ile  Arg Pro Ala 
    1220                 1225                 1230             


Tyr Asp  Lys Leu Thr Pro Glu  Glu Asn Leu Gln Leu  Tyr Asp Ala 
    1235                 1240                 1245             


Phe Thr  Gln Trp Leu Thr Ser  Gly Ile Tyr Ala Lys  Arg Leu Ser 
    1250                 1255                 1260             


Leu Gln  Gly Lys Phe Leu Leu  Glu Lys Arg Asp Ala  Phe Ala Ala 
    1265                 1270                 1275             


Leu Ser  Pro Glu Ala Gln Val  Arg Gln Leu Met Glu  Ile Leu His 
    1280                 1285                 1290             


Leu Phe  Gln Cys Asn Pro Val  Ala Ala Asn Leu Ser  Glu Leu Gly 
    1295                 1300                 1305             


Gly Ala  Ala His Ala Gly Ile  Leu Leu Ala Ser Lys  Asn Ile Asp 
    1310                 1315                 1320             


Gly Lys  Val Pro Val Ser Ile  Val His Gln Ser Val  Thr Gly Tyr 
    1325                 1330                 1335             


Phe Thr  Gln Glu Val Cys Leu  Asn Asp Leu 
    1340                 1345             


<210> 79
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG1-4 (D9A) protospacer and PAM for in vitro
       nickase assay

<400> 79
ccgtgagcca ccacgtcgca agcctcgacc aggaagg                                37


<210> 80
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG1-6 (D13A) protospacer and PAM for in vitro
       nickase assay

<400> 80
tccgtgagcc accacgtcgc aagcctcgac tagaacgg                               38


<210> 81
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG3-6 (D13A) protospacer and PAM for in vitro
       nickase assay

<400> 81
agccaccacg tcgcaagcct cgacgtgggt ta                                     32


<210> 82
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG3-7 (D12A) protospacer and PAM for in vitro
      nickase assay

<400> 82
agccaccacg tcgcaagcct cgacgtgtta c                                      31


<210> 83
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG3-8 (D13A) protospacer and PAM for in vitro
      nickase assay

<400> 83
agccaccacg tcgcaagcct cgacgtgggt ta                                     32


<210> 84
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG4-5 (D17A) protospacer and PAM for in vitro 
      nickase assay

<400> 84
agccaccacg tcgcaagcct cgacggcca                                         29


<210> 85
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG14-1 (D23A) protospacer and PAM for in vitro 
      nickase assay

<400> 85
tccgtgagcc accacgtcgc aagcctcgac ggcggggacg                             40


<210> 86
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG15-1 (D8A) protospacer and PAM for in vitro 
      nickase assay

<400> 86
ccgtgagcca ccacgtcgca agcctcgact ggacctg                                37


<210> 87
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: target sequence

<220>
<223> Description: nMG18-1 (D12A) protospacer and PAM for in vitro 
      nickase assay

<400> 87
agccaccacg tcgcaagcct cgaccgtaat tg                                     32


<210> 88
<211> 121
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG1-4 (D9A) single guide RNA for in vitro 
      nickase assay

<400> 88
ccaccacgtc gcaagcctcg acgttttgat ttactcgaaa gagtccaatc ataattgacc       60

ggagaataat tgattcctct acaatgtacg aataaattca ttctctaaac cttaaaaatt      120

t                                                                      121


<210> 89
<211> 99
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG1-6 (D13A) single guide RNA for in vitro 
      nickase assay

<400> 89
ccaccacgtc gcaagcctcg acgttttgac ttgaaaaagt cttaactgat tttgccgaat       60

ttcaagctct gcattgcacc ttggcattcg gcatatttt                              99


<210> 90
<211> 110
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG3-6 (D13A) single guide RNA for in vitro 
      nickase assay

<400> 90
ccaccacgtc gcaagcctcg acgttgagaa tcgaaagatt cttaataagg catccttccg       60

atgctgactt ctcaccgtcc gttttccaat aggagcgggc ggtatgtttt                 110


<210> 91
<211> 113
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG3-7 (D12A) single guide RNA for in vitro 
      nickase assay

<400> 91
ccaccacgtc gcaagcctcg acgttgggaa ccgaaaggtt cccaataagg cgcatcttgg       60

cgctgacttc tcaccgtcct cttgctgctt agcagagggc ggtatgtttt ttt             113


<210> 92
<211> 118
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG3-8 (D13A) single guide RNA for in vitro 
      nickase assay

<400> 92
ccaccacgtc gcaagcctcg acgttgagaa tctttcgaaa gaaagattct taataaggca       60

tccttccgat gctgacttct caccgtccgg ctcctcttag gaacgggcgg tatgtttt        118


<210> 93
<211> 124
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG4-5 (D17A) single guide RNA for in vitro 
      nickase assay

<400> 93
ccaccacgtc gcaagcctcg acgctgtggc ttgcggggga aaccccttgt cacagtaagg       60

gactttcgtt cgcgaaaggc aacctcgcca gcatcgctgg gcgaggacca gggcaaggcg      120

attt                                                                   124


<210> 94
<211> 118
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG14-1 (D23A) single guide RNA for in vitro 
      nickase assay

<400> 94
ccaccacgtc gcaagcctcg acgtcttgag cgaaagctcc agacaagggg agccacttaa       60

gtggcttacc cgtaaagtaa cccccgttca atcttcggat tgggcggggc gaactttt        118


<210> 95
<211> 118
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG15-1 (D8A) single guide RNA for in vitro 
      nickase assay

<400> 95
ccaccacgtc gcaagcctcg acgttgtaat tccctagaaa taggttatta caataaggtc       60

caacaggagt gttggtaccg taaagctcta acggcaccca cgggtgccgt tatctttt        118


<210> 96
<211> 101
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: single guide RNA

<220>
<223> Description: nMG18-1 (D12A) single guide RNA for in vitro 
      nickase assay

<400> 96
ccaccacgtc gcaagcctcg acgtttgaga gtagtgaaaa ctacgagttc aaatacaatt       60

ttttcaaatt gccctatagg gccctcacag tgtgagattt t                          101


<210> 97
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-4 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 97
cgtttgccgt ctgaatttga cc                                                22


<210> 98
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-4 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 98
gaagatcagg atatgtggcg ga                                                22


<210> 99
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-4 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 99
acgggtaaca gtttctttat gg                                                22


<210> 100
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-6 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 100
cgttgctgca taaaccgact ac                                                22


<210> 101
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-6 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 101
aaaacccgaa actgtggagc gc                                                22


<210> 102
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA1-6 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 102
gattgaaaat ggtctgctgc tg                                                22


<210> 103
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-6 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 103
tacgggtaac agtttcttta tg                                                22


<210> 104
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-6 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 104
gcgttggcaa tttaaccgcc ag                                                22


<210> 105
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-6 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 105
ctgaatatcg acggtttcca ta                                                22


<210> 106
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-7 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 106
cacatttaat gttgatgaaa gc                                                22


<210> 107
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-7 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 107
acacaaatca gcgatttcca tg                                                22


<210> 108
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-7 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 108
atggatgagc agacgatggt gc                                                22


<210> 109
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-8 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 109
ttcatctgtg gtgcaacggg cg                                                22


<210> 110
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-8 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 110
acgagcatca tcctctgcat gg                                                22


<210> 111
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA3-8 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 111
cgacccgcat tgaccctaac gc                                                22


<210> 112
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA4-5 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 112
cacaaatcag cgatttccat gt                                                22


<210> 113
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA4-5 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 113
tgatgaagca gaacaacttt aa                                                22


<210> 114
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA4-5 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 114
aatattgaaa cccacggcat gg                                                22


<210> 115
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA14-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 115
tcaaaaaatg gctttcgcta cc                                                22


<210> 116
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA14-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 116
aagcagcgtt gttgcagtgc ac                                                22


<210> 117
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA14-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 117
tcagccggaa aacctaccgg at                                                22


<210> 118
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA15-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 118
gaagatcagg atatgtggcg ga                                                22


<210> 119
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA15-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 119
aacgtcgaaa acccgaaact gt                                                22


<210> 120
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA15-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 120
aaacccacgg catggtgcca at                                                22


<210> 121
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA18-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 121
aaaacccgaa actgtggagc gc                                                22


<210> 122
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA18-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 122
ggcaactctg gctcacagta cg                                                22


<210> 123
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGA18-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 123
ccggaaaacc taccggattg at                                                22


<210> 124
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: ABE8.17m sgRNA spacer 1 (targeting E. coli lacZ)

<400> 124
tttctttcac agatgtggat                                                   20


<210> 125
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: ABE8.17m sgRNA spacer 2 (targeting E. coli lacZ)

<400> 125
caggatatgt ggcggatgag                                                   20


<210> 126
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: ABE8.17m sgRNA spacer 3 (targeting E. coli lacZ)

<400> 126
tgcgaatacg cccacgcgat                                                   20


<210> 127
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-4 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 127
cggcgtttca tctgtggtgc aa                                                22


<210> 128
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-4 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 128
gccaggacag tcgtttgccg tc                                                22


<210> 129
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-4 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 129
tcagccgcgc tgtactggag gc                                                22


<210> 130
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-6 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 130
cccttcccaa cagttgcgca gc                                                22


<210> 131
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-6 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 131
ctgtcgtcgt cccctcaaac tg                                                22


<210> 132
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC1-6 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 132
ctacgtctga acgtcgaaaa cc                                                22


<210> 133
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-6 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 133
gtgacggcag ttatctggaa ga                                                22


<210> 134
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-6 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 134
atgatttcag ccgcgctgta ct                                                22


<210> 135
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-6 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 135
ttactgccgc ctgttttgac cg                                                22


<210> 136
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-7 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 136
cgaatggcgc tttgcctggt tt                                                22


<210> 137
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-7 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 137
tcccctcaaa ctggcagatg ca                                                22


<210> 138
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-7 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 138
atctacacca acgtgaccta tc                                                22


<210> 139
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-8 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 139
ttcatctgtg gtgcaacggg cg                                                22


<210> 140
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-8 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 140
acgagcatca tcctctgcat gg                                                22


<210> 141
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC3-8 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 141
cgacccgcat tgaccctaac gc                                                22


<210> 142
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC4-5 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 142
caacgggcgc tgggtcggtt ac                                                22


<210> 143
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC4-5 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 143
cacaaatcag cgatttccat gt                                                22


<210> 144
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC4-5 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 144
aaggcggcgg agccgacacc ac                                                22


<210> 145
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC14-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 145
cggtcaatcc gccgtttgtt cc                                                22


<210> 146
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC14-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 146
tcctttgcga atacgcccac gc                                                22


<210> 147
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC14-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 147
ggtcggctta cggcggtgat tt                                                22


<210> 148
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC15-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 148
gccgatcgcg tcacactacg tc                                                22


<210> 149
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC15-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 149
gatccgcgct ggctaccggc ga                                                22


<210> 150
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC15-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 150
ccttcccgcc cggtgcagta tg                                                22


<210> 151
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC18-1 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 151
tcagccgcgc tgtactggag gc                                                22


<210> 152
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC18-1 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 152
gaccagccct tcccggctgt gc                                                22


<210> 153
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: MGC18-1 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 153
tccgtttatc cgggcaaacc at                                                22


<210> 154
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: BE3 sgRNA spacer 1 (targeting E. coli lacZ)

<400> 154
cctctggatg tcgctccaca                                                   20


<210> 155
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: BE3 sgRNA spacer 2 (targeting E. coli lacZ)

<400> 155
gggacgcgcg aattgaatta                                                   20


<210> 156
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: BE3 sgRNA spacer 3 (targeting E. coli lacZ)

<400> 156
cgttttacaa cgtcgtgact                                                   20


<210> 157
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG1-4 (D9A)

<400> 157
tgaaaaagaa agttttcgca tttgcgcttg gtaaagcaag                             40


<210> 158
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG1-4 (D9A)

<400> 158
tggtatatct ccttcttaaa gttaaac                                           27


<210> 159
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG1-6 (D13A)

<400> 159
accatgtttg gggctttgcg attggcaaag ggtct                                  35


<210> 160
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG1-6 (D13A)

<400> 160
tgttcttttc tttctccatg gtata                                             25


<210> 161
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-6 (D13A)

<400> 161
attatcgcat tggcgtggcg gtcggggatc gcagc                                  35


<210> 162
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-6 (D13A)

<400> 162
tcttcatatc ggttgacatg g                                                 21


<210> 163
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-7 (D12A)

<400> 163
agtaccgcat tggcatcgcg gtgggcgatc gctcg                                  35


<210> 164
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-7 (D12A)

<400> 164
tagtatgttc gctcatggta ta                                                22


<210> 165
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-8 (D13A)

<400> 165
attaccgcat tggcgtggcg gtaggtgatc gtagt                                  35


<210> 166
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG3-8 (D13A)

<400> 166
tcttcatatc agtgctcatg g                                                 21


<210> 167
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG4-5 (D17A)

<400> 167
gctactgctt gggcctggcg gtaggtgtgc aagga                                  35


<210> 168
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG4-5 (D17A)

<400> 168
tggtcgcctg cagggtct                                                     18


<210> 169
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG14-1 (D23A)

<400> 169
acctggttct gggtatggcg ccgggcatcg cctca                                  35


<210> 170
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG14-1 (D23A)

<400> 170
ggcgaggcaa gcctaatt                                                     18


<210> 171
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG15-1 (D8A)

<400> 171
actatgtgct cggcctggcg gtcggtattg cttca                                  35


<210> 172
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG15-1 (D8A)

<400> 172
tcatggtata tctccttctt aaagt                                             25


<210> 173
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG18-1 (D12A)

<400> 173
taaagagtac tacattggtc tggcgggtgg tacggactcg                             40


<210> 174
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of MG18-1 (D12A)

<400> 174
ctctggtcca tggtatatct c                                                 21


<210> 175
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of SpCas9 (D10A)

<400> 175
gataagaaat actcaatagg actggcgatt ggcacaaata gc                          42


<210> 176
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Site-directed mutagenesis of SpCas9 (D10A)

<400> 176
catggtatat ctccttctta aagtt                                             25


<210> 177
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: For lacZ sequencing

<400> 177
ccaggcttta cactttatgc t                                                 21


<210> 178
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: For lacZ sequencing

<400> 178
gtatgtggtg gatgaagcc                                                    19


<210> 179
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify the fragment for nickase assay

<400> 179
agcttccagg gggaaacg                                                     18


<210> 180
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify the fragment for nickase assay

<400> 180
attaacctat aaaaataggc gtatca                                            26


<210> 181
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify T7 promoter-His tag-adenine deaminase for  
      MGA entry plasmid

<400> 181
gatataccat gggcagcagt catcatcatc accatcactc cgaggttgaa tttagtcatg       60


<210> 182
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify T7 promoter-His tag-adenine deaminase for  
      MGA entry plasmid

<400> 182
agactttcct cttcttcttg ggagacccgc cagagcctcg aggacccacc agagctgcct       60


<210> 183
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SV40 NLS-vector backbone for  
      MGA entry plasmid

<400> 183
tctggcgggt ctcccaagaa gaagaggaaa gtctaagatc cggctgctaa caaag            55


<210> 184
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SV40 NLS-vector backbone for  
      MGA entry plasmid

<400> 184
ctccttgcat gctctagaat taattaaacc attccttgcg gcggc                       45


<210> 185
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify vector backbone for  
      MGA entry plasmid

<400> 185
aggaatggtt taattaattc tagagcatgc aaggagatgg cgcccaac                    48


<210> 186
<211> 56
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify vector backbone for  
      MGA entry plasmid

<400> 186
gtgatggtga tgatgatgac tgctgcccat ggtatatctc cttcttaaag ttaaac           56


<210> 187
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify T7 promoter-His-tag-cytosine deaminase for 
      MGC entry plasmid

<400> 187
aggagatata ccatgggcag cagtcatcat catcaccatc acatgtcgtc tgagaccggg       60


<210> 188
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify T7 promoter-His-tag-cytosine deaminase for 
      MGC entry plasmid

<400> 188
taatatctga aagattggta gaaccaccag acccgcggca gattcaggtg tcgcactttc       60


<210> 189
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify UGI-SV40 NLS for MGC entry plasmid

<400> 189
cgacacctga atctgccgcg ggtctggtgg ttctaccaat cttt                        44


<210> 190
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify UGI-SV40 NLS for MGC entry plasmid

<400> 190
ttagactttc ctcttcttct tgggagaacc accagaaagc attttgatct tgttttcgcc       60


<210> 191
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SV40 NLS-vector backbone for 
      MGC entry plasmid

<400> 191
ctggtggttc tcccaagaag aagaggaaag tctaagatcc ggctgctaac aaag             54


<210> 192
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SV40 NLS-vector backbone for 
      MGC entry plasmid

<400> 192
ctccttgcat gctctagaat taattaacca ttccttgcgg cggcg                       45


<210> 193
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify vector backbone for MGC entry plasmid

<400> 193
aggaatggtt aattaattct agagcatgca aggagatggc gcccaaca                    48


<210> 194
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify vector backbone for MGC entry plasmid

<400> 194
gtgatgatga tgactgctgc ccatggtata tctccttctt aaagttaaac                  50


<210> 195
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-4 (D9A) for pMGA expression plasmid

<400> 195
aaagtagtgg aggcagctct ggtgggtcca tgaaaaagaa agttttcgca tttgc            55


<210> 196
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-4 (D9A) for pMGA expression plasmid

<400> 196
ctcttcttct tgggagaccc gccagagcct ttgctttcga cttttttaaa ttcgg            55


<210> 197
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-6 (D13A) for pMGA expression plasmid

<400> 197
gaaagtagtg gaggcagctc tggtgggtcc atggagaaag aaaagaacaa ccat             54


<210> 198
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-6 (D13A) for pMGA expression plasmid

<400> 198
cctcttcttc ttgggagacc cgccagagcc cagttcactt aactcgacgc                  50


<210> 199
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-6 (D13A) for pMGA expression plasmid

<400> 199
gaaagtagtg gaggcagctc tggtgggtcc atgtcaaccg atatgaagaa ttatc            55


<210> 200
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-6 (D13A) for pMGA expression plasmid

<400> 200
cctcttcttc ttgggagacc cgccagagcc cccctctact ccaagcgc                    48


<210> 201
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-7 (D12A) for pMGA expression plasmid

<400> 201
gaaagtagtg gaggcagctc tggtgggtcc atgagcgaac atactaagta cc               52


<210> 202
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-7 (D12A) for pMGA expression plasmid

<400> 202
cctcttcttc ttgggagacc cgccagagcc ttcttgttca tcaagggctg                  50


<210> 203
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-8 (D13A) for pMGA expression plasmid

<400> 203
gaaagtagtg gaggcagctc tggtgggtcc atgagcactg atatgaagaa ttac             54


<210> 204
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-8 (D13A) for pMGA expression plasmid

<400> 204
cctcttcttc ttgggagacc cgccagagcc accctcgacg cctaacgc                    48


<210> 205
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG4-5 (D17A) for pMGA expression plasmid

<400> 205
gaaagtagtg gaggcagctc tggtgggtcc atgggagatc agcagacc                    48


<210> 206
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG4-5 (D17A) for pMGA expression plasmid

<400> 206
cctcttcttc ttgggagacc cgccagagcc atcgttcgcg ggaaacac                    48


<210> 207
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG14-1 (D23A) for pMGA expression plasmid

<400> 207
gaaagtagtg gaggcagctc tggtgggtcc atggaaatta caattaaccg tgaga            55


<210> 208
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG14-1 (D23A) for pMGA expression plasmid

<400> 208
cctcttcttc ttgggagacc cgccagagcc cttaatcggg ctctcacg                    48


<210> 209
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG15-1 (D8A) for pMGA expression plasmid

<400> 209
gaaagtagtg gaggcagctc tggtgggtcc atgaactatg tgctcggcc                   49


<210> 210
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG15-1 (D8A) for pMGA expression plasmid

<400> 210
cctcttcttc ttgggagacc cgccagagcc acggaacgtt tggcgctt                    48


<210> 211
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG18-1 (D12A) for pMGA expression plasmid

<400> 211
gaaagtagtg gaggcagctc tggtgggtcc atggaccaga gtaaagagta ct               52


<210> 212
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG18-1 (D12A) for pMGA expression plasmid

<400> 212
cctcttcttc ttgggagacc cgccagagcc cagatcattt aaacacacct cttg             54


<210> 213
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SpCas9 (D10A) for pMGA expression plasmid

<400> 213
gaaagtagtg gaggcagctc tggtgggtcc atggataaga aatactcaat aggac            55


<210> 214
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SpCas9 (D10A) for pMGA expression plasmid

<400> 214
cctcttcttc ttgggagacc cgccagagcc gtcacctccc agctgact                    48


<210> 215
<211> 56
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-4 (D9A) for pMGC expression plasmid

<400> 215
acatccgaaa gtgcgacacc tgaatctgcc atgaaaaaga aagttttcgc atttgc           56


<210> 216
<211> 56
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-4 (D9A) for pMGC expression plasmid

<400> 216
atctgaaaga ttggtagaac caccagaccc tttgctttcg acttttttaa attcgg           56


<210> 217
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-6 (D13A) for pMGC expression plasmid

<400> 217
acatccgaaa gtgcgacacc tgaatctgcc atggagaaag aaaagaacaa ccat             54


<210> 218
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG1-6 (D13A) for pMGC expression plasmid

<400> 218
atctgaaaga ttggtagaac caccagaccc cagttcactt aactcgacgc                  50


<210> 219
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-6 (D13A) for pMGC expression plasmid

<400> 219
catccgaaag tgcgacacct gaatctgcca tgtcaaccga tatgaagaat tatc             54


<210> 220
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-6 (D13A) for pMGC expression plasmid

<400> 220
atctgaaaga ttggtagaac caccagaccc cccctctact ccaagcgc                    48


<210> 221
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-7 (D12A) for pMGC expression plasmid

<400> 221
acatccgaaa gtgcgacacc tgaatctgcc atgagcgaac atactaagta cc               52


<210> 222
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-7 (D12A) for pMGC expression plasmid

<400> 222
atctgaaaga ttggtagaac caccagaccc ttcttgttca tcaagggctg                  50


<210> 223
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-8 (D13A) for pMGC expression plasmid

<400> 223
acatccgaaa gtgcgacacc tgaatctgcc atgagcactg atatgaagaa ttac             54


<210> 224
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG3-8 (D13A) for pMGC expression plasmid

<400> 224
atctgaaaga ttggtagaac caccagaccc accctcgacg cctaacgc                    48


<210> 225
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG4-5 (D17A) for pMGC expression plasmid

<400> 225
acatccgaaa gtgcgacacc tgaatctgcc atgggagatc agcagacc                    48


<210> 226
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG4-5 (D17A) for pMGC expression plasmid

<400> 226
atctgaaaga ttggtagaac caccagaccc atcgttcgcg ggaaacac                    48


<210> 227
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG14-1 (D23A) for pMGC expression plasmid

<400> 227
acatccgaaa gtgcgacacc tgaatctgcc atggaaatta caattaaccg tgag             54


<210> 228
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG14-1 (D23A) for pMGC expression plasmid

<400> 228
atctgaaaga ttggtagaac caccagaccc cttaatcggg ctctcacga                   49


<210> 229
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG15-1 (D8A) for pMGC expression plasmid

<400> 229
acatccgaaa gtgcgacacc tgaatctgcc atgaactatg tgctcggcc                   49


<210> 230
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG15-1 (D8A) for pMGC expression plasmid

<400> 230
atctgaaaga ttggtagaac caccagaccc acggaacgtt tggcgctt                    48


<210> 231
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG18-1 (D12A) for pMGC expression plasmid

<400> 231
atccgaaagt gcgacacctg aatctgccat ggaccagagt aaagagtac                   49


<210> 232
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify nMG18-1 (D12A) for pMGC expression plasmid

<400> 232
tatctgaaag attggtagaa ccaccagacc ccagatcatt taaacacacc tcttg            55


<210> 233
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SpCas9 (D10A) for pMGC expression plasmid

<400> 233
acatccgaaa gtgcgacacc tgaatctgcc atggataaga aatactcaat aggac            55


<210> 234
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify SpCas9 (D10A) for pMGC expression plasmid

<400> 234
atctgaaaga ttggtagaac caccagaccc gtcacctccc agctgact                    48


<210> 235
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 1

<400> 235
ggtcaaattc agacggcaaa cgctatagtg agtcgtatta aggt                        44


<210> 236
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 1

<400> 236
tatagcgttt gccgtctgaa tttgaccgtt ttgatttact cgaaagagtc                  50


<210> 237
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 2

<400> 237
caaaactccg ccacatatcc tgatcttcct atagtgagtc gtattaaggt                  50


<210> 238
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 2

<400> 238
tataggaaga tcaggatatg tggcggagtt ttgatttact cgaaagagtc                  50


<210> 239
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 3

<400> 239
caaaacccat aaagaaactg ttacccgtct atagtgagtc gtattaaggt                  50


<210> 240
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-4_sgRNA spacer 3

<400> 240
tatagacggg taacagtttc tttatgggtt ttgatttact cgaaagagtc                  50


<210> 241
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 1

<400> 241
caaaacgtag tcggtttatg cagcaacgct atagtgagtc gtattaaggt                  50


<210> 242
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 1

<400> 242
tagcgttgct gcataaaccg actacgtttt gacttgaaaa agtcttaact                  50


<210> 243
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 2

<400> 243
caaaacgcgc tccacagttt cgggttttct atagtgagtc gtattaaggt                  50


<210> 244
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 2

<400> 244
tagaaaaccc gaaactgtgg agcgcgtttt gacttgaaaa agtcttaact                  50


<210> 245
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 3

<400> 245
caaaaccagc agcagaccat tttcaatcct atagtgagtc gtattaaggt                  50


<210> 246
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA1-6_sgRNA spacer 3

<400> 246
taggattgaa aatggtctgc tgctggtttt gacttgaaaa agtcttaact                  50


<210> 247
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 1

<400> 247
ctcaaccata aagaaactgt tacccgtact atagtgagtc gtattaaggt                  50


<210> 248
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 1

<400> 248
atagtacggg taacagtttc tttatggttg agaatcgaaa gattcttaat                  50


<210> 249
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 2

<400> 249
ctcaacctgg cggttaaatt gccaacgcct atagtgagtc gtattaaggt                  50


<210> 250
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 2

<400> 250
taggcgttgg caatttaacc gccaggttga gaatcgaaag attcttaata                  50


<210> 251
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 3

<400> 251
ctcaactatg gaaaccgtcg atattcagct atagtgagtc gtattaaggt                  50


<210> 252
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-6_sgRNA spacer 3

<400> 252
tagctgaata tcgacggttt ccatagttga gaatcgaaag attcttaata                  50


<210> 253
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 1

<400> 253
cccaacgctt tcatcaacat taaatgtgct atagtgagtc gtattaaggt                  50


<210> 254
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 1

<400> 254
tcactatagc acatttaatg ttgatgaaag cgttgggaac cgaaaggttc                  50


<210> 255
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 2

<400> 255
cccaaccatg gaaatcgctg atttgtgtct atagtgagtc gtattaaggt                  50


<210> 256
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 2

<400> 256
tcactataga cacaaatcag cgatttccat ggttgggaac cgaaaggttc                  50


<210> 257
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 3

<400> 257
cccaacgcac catcgtctgc tcatccatct atagtgagtc gtattaaggt                  50


<210> 258
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-7_sgRNA spacer 3

<400> 258
tcactataga tggatgagca gacgatggtg cgttgggaac cgaaaggttc                  50


<210> 259
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 1

<400> 259
cacagcacat ggaaatcgct gatttgtgct atagtgagtc gtattaaggt                  50


<210> 260
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 1

<400> 260
cactatagca caaatcagcg atttccatgt gctgtggctt gcggggga                    48


<210> 261
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 2

<400> 261
cacagcttaa agttgttctg cttcatcact atagtgagtc gtattaaggt                  50


<210> 262
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 2

<400> 262
cactatagtg atgaagcaga acaactttaa gctgtggctt gcggggga                    48


<210> 263
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 3

<400> 263
cacagcccat gccgtgggtt tcaatattct atagtgagtc gtattaaggt                  50


<210> 264
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA4-5_sgRNA spacer 3

<400> 264
cactatagaa tattgaaacc cacggcatgg gctgtggctt gcggggga                    48


<210> 265
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 1

<400> 265
caagacggta gcgaaagcca ttttttgact atagtgagtc gtattaaggt                  50


<210> 266
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 1

<400> 266
cactatagtc aaaaaatggc tttcgctacc gtcttgagcg aaagctcc                    48


<210> 267
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 2

<400> 267
caagacgtgc actgcaacaa cgctgcttct atagtgagtc gtattaaggt                  50


<210> 268
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 2

<400> 268
cactatagaa gcagcgttgt tgcagtgcac gtcttgagcg aaagctcc                    48


<210> 269
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 3

<400> 269
caagacatcc ggtaggtttt ccggctgact atagtgagtc gtattaaggt                  50


<210> 270
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA14-1_sgRNA spacer 3

<400> 270
cactatagtc agccggaaaa cctaccggat gtcttgagcg aaagctcc                    48


<210> 271
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 1

<400> 271
tacaactccg ccacatatcc tgatcttcct atagtgagtc gtattaaggt                  50


<210> 272
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 1

<400> 272
tataggaaga tcaggatatg tggcggagtt gtaattccct agaaataggt                  50


<210> 273
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 2

<400> 273
tacaacacag tttcgggttt tcgacgttct atagtgagtc gtattaaggt                  50


<210> 274
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 2

<400> 274
tatagaacgt cgaaaacccg aaactgtgtt gtaattccct agaaataggt                  50


<210> 275
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 3

<400> 275
tacaacattg gcaccatgcc gtgggtttct atagtgagtc gtattaaggt                  50


<210> 276
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA15-1_sgRNA spacer 3

<400> 276
tatagaaacc cacggcatgg tgccaatgtt gtaattccct agaaataggt                  50


<210> 277
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 1

<400> 277
tcaaacgcgc tccacagttt cgggttttct atagtgagtc gtattaaggt                  50


<210> 278
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 1

<400> 278
tatagaaaac ccgaaactgt ggagcgcgtt tgagagtagt gaaaactacg                  50


<210> 279
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 2

<400> 279
tcaaaccgta ctgtgagcca gagttgccct atagtgagtc gtattaaggt                  50


<210> 280
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 2

<400> 280
tatagggcaa ctctggctca cagtacggtt tgagagtagt gaaaactacg                  50


<210> 281
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 3

<400> 281
tcaaacatca atccggtagg ttttccggct atagtgagtc gtattaaggt                  50


<210> 282
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA18-1_sgRNA spacer 3

<400> 282
tatagccgga aaacctaccg gattgatgtt tgagagtagt gaaaactacg                  50


<210> 283
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 1

<400> 283
atccacatct gtgaaagaaa ctatagtgag tcgtattagg a                           41


<210> 284
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 1

<400> 284
tttctttcac agatgtggat gttttagagc tagaaatagc aagtt                       45


<210> 285
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 2

<400> 285
ctcatccgcc acatatcctg ctatagtgag tcgtattagg a                           41


<210> 286
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 2

<400> 286
caggatatgt ggcggatgag gttttagagc tagaaatagc aagtt                       45


<210> 287
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 3

<400> 287
atcgcgtggg cgtattcgca ctatagtgag tcgtattagg a                           41


<210> 288
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify ABE8.17m_sgRNA spacer 3

<400> 288
tgcgaatacg cccacgcgat gttttagagc tagaaatagc aagtt                       45


<210> 289
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 1

<400> 289
caaaacttgc accacagatg aaacgccgct atagtgagtc gtattaaggt                  50


<210> 290
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 1

<400> 290
tatagcggcg tttcatctgt ggtgcaagtt ttgatttact cgaaagagtc                  50


<210> 291
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 2

<400> 291
caaaacgacg gcaaacgact gtcctggcct atagtgagtc gtattaaggt                  50


<210> 292
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 2

<400> 292
tataggccag gacagtcgtt tgccgtcgtt ttgatttact cgaaagagtc                  50


<210> 293
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 3

<400> 293
caaaacgcct ccagtacagc gcggctgact atagtgagtc gtattaaggt                  50


<210> 294
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-4_spacer 3

<400> 294
tatagtcagc cgcgctgtac tggaggcgtt ttgatttact cgaaagagtc                  50


<210> 295
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 1

<400> 295
caaaacgctg cgcaactgtt gggaagggct atagtgagtc gtattaaggt                  50


<210> 296
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 1

<400> 296
tagcccttcc caacagttgc gcagcgtttt gacttgaaaa agtcttaact                  50


<210> 297
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 2

<400> 297
caaaaccagt ttgaggggac gacgacagct atagtgagtc gtattaaggt                  50


<210> 298
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 2

<400> 298
tagctgtcgt cgtcccctca aactggtttt gacttgaaaa agtcttaact                  50


<210> 299
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 3

<400> 299
caaaacggtt ttcgacgttc agacgtagct atagtgagtc gtattaaggt                  50


<210> 300
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC1-6_spacer 3

<400> 300
tagctacgtc tgaacgtcga aaaccgtttt gacttgaaaa agtcttaact                  50


<210> 301
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 1

<400> 301
ctcaactctt ccagataact gccgtcacct atagtgagtc gtattaaggt                  50


<210> 302
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 1

<400> 302
aggtgacggc agttatctgg aagagttgag aatcgaaaga ttcttaataa                  50


<210> 303
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 2

<400> 303
tcaacagtac agcgcggctg aaatcatcta tagtgagtcg tattaaggtc                  50


<210> 304
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 2

<400> 304
agatgatttc agccgcgctg tactgttgag aatcgaaaga ttcttaataa                  50


<210> 305
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 3

<400> 305
ctcaaccggt caaaacaggc ggcagtaact atagtgagtc gtattaaggt                  50


<210> 306
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-6_spacer 3

<400> 306
tagttactgc cgcctgtttt gaccggttga gaatcgaaag attcttaata                  50


<210> 307
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 1

<400> 307
cccaacaaac caggcaaagc gccattcgct atagtgagtc gtattaaggt                  50


<210> 308
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 1

<400> 308
cactatagcg aatggcgctt tgcctggttt gttgggaacc gaaaggttc                   49


<210> 309
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 2

<400> 309
cccaactgca tctgccagtt tgaggggact atagtgagtc gtattaaggt                  50


<210> 310
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 2

<400> 310
cactatagtc ccctcaaact ggcagatgca gttgggaacc gaaaggttc                   49


<210> 311
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 3

<400> 311
cccaacgata ggtcacgttg gtgtagatct atagtgagtc gtattaaggt                  50


<210> 312
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-7_spacer 3

<400> 312
cactatagat ctacaccaac gtgacctatc gttgggaacc gaaaggttc                   49


<210> 313
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 1

<400> 313
cacagcgtaa ccgacccagc gcccgttgct atagtgagtc gtattaaggt                  50


<210> 314
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 1

<400> 314
cactatagca acgggcgctg ggtcggttac gctgtggctt gcggggga                    48


<210> 315
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 2

<400> 315
cacagcacat ggaaatcgct gatttgtgct atagtgagtc gtattaaggt                  50


<210> 316
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 2

<400> 316
cactatagca caaatcagcg atttccatgt gctgtggctt gcggggga                    48


<210> 317
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 3

<400> 317
cacagcgtgg tgtcggctcc gccgccttct atagtgagtc gtattaaggt                  50


<210> 318
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC4-5_spacer 3

<400> 318
cactatagaa ggcggcggag ccgacaccac gctgtggctt gcggggga                    48


<210> 319
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 1

<400> 319
caagacggaa caaacggcgg attgaccgct atagtgagtc gtattaaggt                  50


<210> 320
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 1

<400> 320
cactatagcg gtcaatccgc cgtttgttcc gtcttgagcg aaagctcc                    48


<210> 321
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 2

<400> 321
caagacgcgt gggcgtattc gcaaaggact atagtgagtc gtattaaggt                  50


<210> 322
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 2

<400> 322
cactatagtc ctttgcgaat acgcccacgc gtcttgagcg aaagctcc                    48


<210> 323
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 3

<400> 323
caagacaaat caccgccgta agccgaccct atagtgagtc gtattaaggt                  50


<210> 324
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC14-1_spacer 3

<400> 324
cactataggg tcggcttacg gcggtgattt gtcttgagcg aaagctcc                    48


<210> 325
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 1

<400> 325
tacaacgacg tagtgtgacg cgatcggcct atagtgagtc gtattaaggt                  50


<210> 326
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 1

<400> 326
ataggccgat cgcgtcacac tacgtcgttg taattcccta gaaataggtt                  50


<210> 327
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 2

<400> 327
tacaactcgc cggtagccag cgcggatcct atagtgagtc gtattaaggt                  50


<210> 328
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 2

<400> 328
ataggatccg cgctggctac cggcgagttg taattcccta gaaataggtt                  50


<210> 329
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 3

<400> 329
tacaaccata ctgcaccggg cgggaaggct atagtgagtc gtattaaggt                  50


<210> 330
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC15-1_spacer 3

<400> 330
atagccttcc cgcccggtgc agtatggttg taattcccta gaaataggtt                  50


<210> 331
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 1

<400> 331
tcaaacgcct ccagtacagc gcggctgact atagtgagtc gtattaaggt                  50


<210> 332
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 1

<400> 332
ctatagtcag ccgcgctgta ctggaggcgt ttgagagtag tgaaaactac                  50


<210> 333
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 2

<400> 333
tcaaacgcac agccgggaag ggctggtcct atagtgagtc gtattaaggt                  50


<210> 334
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 2

<400> 334
ctataggacc agcccttccc ggctgtgcgt ttgagagtag tgaaaactac                  50


<210> 335
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 3

<400> 335
tcaaacatgg tttgcccgga taaacggact atagtgagtc gtattaaggt                  50


<210> 336
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC18-1_spacer 3

<400> 336
ctatagtccg tttatccggg caaaccatgt ttgagagtag tgaaaactac                  50


<210> 337
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 1

<400> 337
tgtggagcga catccagagg ctatagtgag tcgtattagg a                           41


<210> 338
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 1

<400> 338
cctctggatg tcgctccaca gttttagagc tagaaatagc aagtt                       45


<210> 339
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 2

<400> 339
taattcaatt cgcgcgtccc ctatagtgag tcgtattagg a                           41


<210> 340
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 2

<400> 340
gggacgcgcg aattgaatta gttttagagc tagaaatagc aagtt                       45


<210> 341
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 3

<400> 341
agtcacgacg ttgtaaaacg ctatagtgag tcgtattagg a                           41


<210> 342
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify BE3_sgRNA spacer 3

<400> 342
cgttttacaa cgtcgtgact gttttagagc tagaaatagc aagtt                       45


<210> 343
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: For lacZ sequencing

<400> 343
cgaacatcca aaagtttgtg ttttt                                             25


<210> 344
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: For lacZ sequencing

<400> 344
tgagcgcatt tttacgcgc                                                    19


<210> 345
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: For lacZ sequencing

<400> 345
gaaaacggca acccgtgg                                                     18


<210> 346
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify sgRNA expression cassette

<400> 346
ccgccgccgc aaggaatggt ttaattaatt acggccagtc atgcataatc                  50


<210> 347
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify sgRNA expression cassette

<400> 347
gactgttggg cgccatctcc ttgcatgctc actgagcctc cacctagcct                  50


<210> 348
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 1

<400> 348
ctcaaccgcc cgttgcacca cagatgaact atagtgagtc gtattaaggt                  50


<210> 349
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 1

<400> 349
tatagttcat ctgtggtgca acgggcggtt gagaatcttt cgaaagaaag                  50


<210> 350
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 2

<400> 350
ctcaacccat gcagaggatg atgctcgtct atagtgagtc gtattaaggt                  50


<210> 351
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 2

<400> 351
atagacgagc atcatcctct gcatgggttg agaatctttc gaaagaaaga                  50


<210> 352
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 3

<400> 352
ctcaacgcgt tagggtcaat gcgggtcgct atagtgagtc gtattaaggt                  50


<210> 353
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGA3-8_sgRNA spacer 3

<400> 353
tatagcgacc cgcattgacc ctaacgcgtt gagaatcttt cgaaagaaag                  50


<210> 354
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 1

<400> 354
ctcaaccgcc cgttgcacca cagatgaact atagtgagtc gtattaaggt                  50


<210> 355
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 1

<400> 355
tatagttcat ctgtggtgca acgggcggtt gagaatcttt cgaaagaaag                  50


<210> 356
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 2

<400> 356
ctcaacccat gcagaggatg atgctcgtct atagtgagtc gtattaaggt                  50


<210> 357
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 2

<400> 357
atagacgagc atcatcctct gcatgggttg agaatctttc gaaagaaaga                  50


<210> 358
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 3

<400> 358
ctcaacgcgt tagggtcaat gcgggtcgct atagtgagtc gtattaaggt                  50


<210> 359
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Amplify MGC3-8_sgRNA spacer 3

<400> 359
tatagcgacc cgcattgacc ctaacgcgtt gagaatcttt cgaaagaaag                  50


<210> 360
<211> 4
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG1-4 (D9A) nickase PAM


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<400> 360
nrrr                                                                     4


<210> 361
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG1-6 (D13A) nickase PAM


<220>
<221> modified_base
<222> (1)..(2)
<223> a, c, t, g, unknown or other

<400> 361
nnrray                                                                   6


<210> 362
<211> 7
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG3-6 (D13A) nickase PAM


<220>
<221> modified_base
<222> (1)..(2)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (6)..(6)
<223> a, c, t, g, unknown or other

<400> 362
nnrggnt                                                                  7


<210> 363
<211> 7
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG3-7 (D12A) nickase PAM


<220>
<221> modified_base
<222> (1)..(2)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (4)..(4)
<223> a, c, t, g, unknown or other

<400> 363
nnrnyay                                                                  7


<210> 364
<211> 7
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG3-8 (D13A) nickase PAM


<220>
<221> modified_base
<222> (1)..(2)
<223> a, c, t, g, unknown or other

<400> 364
nnrggty                                                                  7


<210> 365
<211> 5
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG4-5 (D17A) nickase PAM


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<400> 365
nrccv                                                                    5


<210> 366
<211> 8
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG14-1 (D23A) nickase PAM


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (3)..(4)
<223> a, c, t, g, unknown or other

<400> 366
nrnngrka                                                                 8


<210> 367
<211> 5
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG15-1 (D8A) nickase PAM


<220>
<221> modified_base
<222> (1)..(4)
<223> a, c, t, g, unknown or other

<400> 367
nnnnc                                                                    5


<210> 368
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: PAM

<220>
<223> Description: nMG18-1 (D12A) nickase PAM


<220>
<221> modified_base
<222> (1)..(1)
<223> a, c, t, g, unknown or other

<400> 368
nrwart                                                                   6


<210> 369
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Category: NLS

<220>
<223> Description: SV40

<400> 369
Pro Lys Lys Lys Arg Lys Val 
1               5           


<210> 370
<211> 16
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      nucleoplasmin bipartite NLS sequence

<220>
<223> Category: NLS

<220>
<223> Description: nucleoplasmin bipartite NLS

<400> 370
Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210> 371
<211> 9
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      c-myc NLS sequence

<220>
<223> Category: NLS

<220>
<223> Description: c-myc NLS

<400> 371
Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210> 372
<211> 11
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      c-myc NLS sequence

<220>
<223> Category: NLS

<220>
<223> Description: c-myc NLS

<400> 372
Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210> 373
<211> 38
<212> PRT
<213> Homo sapiens

<220>
<223> Category: NLS

<220>
<223> Description: hRNPA1 M9 NLS

<400> 373
Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210> 374
<211> 42
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      Importin-alpha IBB domain sequence

<220>
<223> Category: NLS

<220>
<223> Description: Importin-alpha IBB domain

<400> 374
Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210> 375
<211> 8
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      Myoma T protein sequence

<220>
<223> Category: NLS

<220>
<223> Description: Myoma T protein

<400> 375
Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210> 376
<211> 8
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      Myoma T protein sequence

<220>
<223> Category: NLS

<220>
<223> Description: Myoma T protein

<400> 376
Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210> 377
<211> 8
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      p53 sequence

<220>
<223> Category: NLS

<220>
<223> Description: p53

<400> 377
Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210> 378
<211> 12
<212> PRT
<213> Mus sp.

<220>
<223> Category: NLS

<220>
<223> Description: mouse c-abl IV

<400> 378
Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210> 379
<211> 5
<212> PRT
<213> Influenza virus

<220>
<223> Category: NLS

<220>
<223> Description: influenza virus NS1

<400> 379
Asp Arg Leu Arg Arg 
1               5   


<210> 380
<211> 7
<212> PRT
<213> Influenza virus

<220>
<223> Category: NLS

<220>
<223> Description: influenza virus NS1

<400> 380
Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210> 381
<211> 10
<212> PRT
<213> Hepatitis delta virus

<220>
<223> Category: NLS

<220>
<223> Description: Hepatitis virus delta antigen

<400> 381
Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210> 382
<211> 10
<212> PRT
<213> Mus sp.

<220>
<223> Category: NLS

<220>
<223> Description: mouse Mx1 protein

<400> 382
Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210> 383
<211> 20
<212> PRT
<213> Homo sapiens

<220>
<223> Category: NLS

<220>
<223> Description: human poly(ADP-ribose) polymerase

<400> 383
Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210> 384
<211> 17
<212> PRT
<213> Homo sapiens

<220>
<223> Category: NLS

<220>
<223> Description: steroid hormone receptor (human) glucocorticoid

<400> 384
Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210> 385
<211> 170
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-3 deaminase

<400> 385
Met Asn Glu Gln Thr Ser Thr Asp Glu Gln Phe Met Gln Gln Ala Leu 
1               5                   10                  15      


Ala Leu Ala Asp Glu Ala Glu Arg Gln Gly Glu Val Pro Val Gly Ala 
            20                  25                  30          


Leu Leu Val His Gln Gly Thr Val Ile Gly Arg Gly Phe Asn Gln Val 
        35                  40                  45              


Ile Thr Gln Leu Asp Pro Ser Ala His Ala Glu Met Leu Ala Ile Arg 
    50                  55                  60                  


Asp Ala Ala Arg Tyr Leu Gln Asn Tyr Arg Ile Leu Asp Thr Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Pro Met Cys Ala Gly Ala Leu Val His 
                85                  90                  95      


Ala Arg Val Lys Arg Leu Val Tyr Gly Ala Ser Asp Pro Arg Thr Gly 
            100                 105                 110         


Ala Cys Gly Ser Val Phe Asn Val Val Ala Asp Thr Arg Leu Asn His 
        115                 120                 125             


Gln Leu Glu Val Val Ala Gly Val Leu Gly Ser Gln Cys Gly Glu Arg 
    130                 135                 140                 


Leu Ser Ala Phe Phe Arg Gln Arg Arg Ala Ala Gln Lys Ala Leu Lys 
145                 150                 155                 160 


Lys Ala Ala Gln Asn Phe Gln Ser Pro Ser 
                165                 170 


<210> 386
<211> 167
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4 deaminase

<400> 386
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asp Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 387
<211> 173
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-5 deaminase

<400> 387
Met Ser Thr Asp Phe Thr Ser Ala Ala Gln Gln Glu Leu Asp Ile Ala 
1               5                   10                  15      


Tyr Met Arg Lys Ala Ile Ala Leu Ala Ala Gln Ala Glu Ala Asp Gly 
            20                  25                  30          


Glu Val Pro Val Gly Ala Val Leu Val His Asn Asn Glu Ile Ile Gly 
        35                  40                  45              


Val Gly Arg Asn Arg Val Ile Gly Gly Cys Asp Pro Ser Leu His Ala 
    50                  55                  60                  


Glu Phe Glu Ala Ile Arg Gln Gly Ala Gln Glu Ile Gly Asn Tyr Arg 
65                  70                  75                  80  


Leu Leu Glu Thr Thr Leu Tyr Val Thr Leu Glu Pro Cys Pro Met Cys 
                85                  90                  95      


Ala Gly Leu Leu Val His Ser Arg Ile Gly Arg Leu Val Tyr Gly Thr 
            100                 105                 110         


Pro Asp Leu Lys Thr Gly Ala Ala Gly Ser Leu Met Asn Leu Leu Gln 
        115                 120                 125             


His Pro Glu Leu Asn His Gln Ile Ala Ile Thr Ser Gly Val Leu Gln 
    130                 135                 140                 


Ala Asp Cys Ala Ala Gln Leu Ser Gly Phe Phe Ser Ala Gln Arg Glu 
145                 150                 155                 160 


Arg Lys Lys Ala Leu Lys Lys Arg Leu Lys Pro Asn Leu 
                165                 170             


<210> 388
<211> 182
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-6 deaminase

<400> 388
Met Ser Glu Ile Pro Gln Val Val Thr Glu Gln Gln Thr Asp Glu Arg 
1               5                   10                  15      


Trp Met His Tyr Ala Leu Ala Leu Ala Asp Lys Ala Glu Gln Ala Gly 
            20                  25                  30          


Glu Ile Pro Val Gly Ala Val Leu Val Lys Asp Asn Gln Val Ile Gly 
        35                  40                  45              


Glu Gly Trp Asn Met Ser Ile Cys Arg His Asp Pro Ser Ala His Ala 
    50                  55                  60                  


Glu Met Leu Ala Ile Arg Glu Ala Ala Val Arg Val Gln Asn Tyr Arg 
65                  70                  75                  80  


Leu Leu Asp Thr Thr Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys 
                85                  90                  95      


Ala Gly Leu Leu Val His Ser Arg Val Lys Arg Leu Val Phe Gly Ala 
            100                 105                 110         


Phe Asp Ala Lys Thr Gly Ala Ala Gly Ser Val Thr Asp Leu Val Arg 
        115                 120                 125             


His Pro Val Leu Asn His Gln Leu Glu Val Thr Ser Gly Val Leu Ala 
    130                 135                 140                 


Glu Gln Cys Ala Asp Lys Leu Ser Ser Phe Phe Arg Arg Arg Arg Gln 
145                 150                 155                 160 


Glu His Lys Ala Ala Lys Ala Leu Arg Gln Ala Gln Asp Pro Glu Asn 
                165                 170                 175     


Gln Gly Asn Ser Glu Ser 
            180         


<210> 389
<211> 151
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-7 deaminase

<400> 389
Met Ser Asp Glu Ile Trp Ile Lys His Ala Leu Thr Leu Ala Asp Lys 
1               5                   10                  15      


Ala Tyr Gln Gln Gly Glu Val Pro Val Gly Ala Val Leu Val Lys Asn 
            20                  25                  30          


Asn Gln Ile Ile Ala Glu Gly Trp Asn Gln Pro Ile Ala His His Asp 
        35                  40                  45              


Pro Ser Ala His Ala Glu Ile Met Ala Leu Arg Leu Ala Gly Gln Ala 
    50                  55                  60                  


Glu Gln Asn Tyr Arg Leu Pro Glu Thr Thr Leu Tyr Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ala Arg Ile Gly Arg 
                85                  90                  95      


Leu Val Phe Gly Ala Tyr Asp Pro Lys Thr Gly Ala Ala Gly Ser Val 
            100                 105                 110         


Phe Asp Ile Phe Ser Asp Ala Arg His Asn His Gln Ile Glu Val Val 
        115                 120                 125             


Ser Gly Val Leu Ser Glu Leu Cys Gly Asp Lys Leu Arg Ala Phe Phe 
    130                 135                 140                 


Arg Glu Arg Arg Lys Lys Arg 
145                 150     


<210> 390
<211> 166
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-8 deaminase

<400> 390
Met Pro Gln Ser Glu Gln Asp Ile Tyr Trp Ile Arg Tyr Ala Met Gln 
1               5                   10                  15      


Leu Ala Glu Arg Ala Glu Gln Ala Gly Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val Leu Asp Asn Gln Ala Leu Gly Glu Gly Trp Asn Leu Ser Ile 
        35                  40                  45              


Ser Arg His Asp Pro Cys Ala His Ala Glu Ile Met Ala Ile Arg Gln 
    50                  55                  60                  


Ala Gly Glu Arg Ile Gly Asn Tyr Arg Leu Leu Gly Ala Thr Leu Tyr 
65                  70                  75                  80  


Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Val His Ser 
                85                  90                  95      


Arg Ile Ala Arg Val Val Tyr Gly Ala Ser Asp Leu Lys Thr Gly Ala 
            100                 105                 110         


Ala Gly Ser Val Phe Asp Val Leu Ser Asp Pro Arg His Asn His Val 
        115                 120                 125             


Val Glu Ile Thr Gly Gly Val Glu Ser Glu Ala Cys Ser Gln Gln Leu 
    130                 135                 140                 


Ser Asp Phe Phe Arg Arg Arg Arg Ala Glu Gln Lys Ala Glu Lys Gln 
145                 150                 155                 160 


Arg Leu Lys Asp Asn Gln 
                165     


<210> 391
<211> 168
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-9 deaminase

<400> 391
Met Asn Val Ala Ala Asp Glu Gln Trp Met Lys Glu Ala Leu Arg Gln 
1               5                   10                  15      


Ala Glu Arg Ala Leu Leu Ala Gly Glu Val Pro Val Gly Ala Val Val 
            20                  25                  30          


Val Ser Gln Asn Asn Ile Val Gly Gln Gly Phe Asn Arg Asn Leu Leu 
        35                  40                  45              


Asp Arg Asp Pro Thr Ala His Ala Glu Ile Ile Ala Leu Arg Glu Ala 
    50                  55                  60                  


Ala Ala Asn Ile Gly Asn His Arg Leu Leu Asp Cys Glu Leu Tyr Ala 
65                  70                  75                  80  


Thr Ile Glu Pro Cys Ala Met Cys Ala Gly Ala Ile Thr His Ala Arg 
                85                  90                  95      


Val Arg Arg Leu Val Tyr Gly Ala Glu Asp Leu Lys Ala Gly Ala Val 
            100                 105                 110         


His Ser Val Met Gln Val Val Asn His Pro Ala Leu Asn His Arg Val 
        115                 120                 125             


Glu Val Lys Gly Gly Val Leu Ala Glu Arg Cys Gly Glu Leu Leu Gln 
    130                 135                 140                 


Gln Phe Phe Trp Ala Arg Arg Ala Glu Gln Ser Gly Ile Ser Gly Ala 
145                 150                 155                 160 


Asn Glu Asp Gln Leu Arg Arg Gly 
                165             


<210> 392
<211> 143
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-10 deaminase

<400> 392
Met Leu Leu Ala Met Glu Gln Ala Arg Ala Ala Glu Glu Ala Gly Glu 
1               5                   10                  15      


Val Pro Val Gly Ala Val Ile Leu Ser Pro Glu Gly Glu Val Val Ala 
            20                  25                  30          


Arg Gly Glu Asn Arg Val Leu Arg Asp Ser Asp Pro Thr Ala His Ala 
        35                  40                  45              


Glu Met Val Ala Leu Arg Ala Ala Gly Arg Val Leu Gly Asn Tyr Arg 
    50                  55                  60                  


Leu Pro Gly Cys Thr Leu Tyr Ser Thr Leu Glu Pro Cys Ser Met Cys 
65                  70                  75                  80  


Ser Gly Ala Ile Leu His Ala Arg Val Ala Arg Leu Val Phe Ala Ala 
                85                  90                  95      


Ser Asp Pro Lys Ala Gly Ala Cys Gly Ser Val Leu Glu Val Met Asn 
            100                 105                 110         


His Pro Arg Leu Asn His Gln Val Glu Leu Val Gly Gly Val Leu Ala 
        115                 120                 125             


Leu Glu Cys Gly Gln Met Leu Thr Glu Phe Phe Arg Ala Arg Arg 
    130                 135                 140             


<210> 393
<211> 210
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-11 deaminase

<400> 393
Met Ser Ala Glu Ile Asp Leu Thr Asp Lys Ala Phe Met Arg Gln Ala 
1               5                   10                  15      


Leu Asp Gln Ala Gln Asn Ala Trp Leu Leu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Val Ile Val Arg Glu Gly Arg Val Ile Ala Thr Gly Tyr Asn Arg 
        35                  40                  45              


Pro Ile Gly Asp Asn Asp Pro Thr Ala His Ala Glu Ile Val Ala Leu 
    50                  55                  60                  


Arg Gln Ala Ala Ser Leu Leu Gly Asn Tyr Arg Leu Pro Gly Cys Thr 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Met Ala Leu Met 
                85                  90                  95      


His Ala Arg Leu Asp Arg Val Val Phe Gly Ala Pro Asp Pro Lys Thr 
            100                 105                 110         


Gly Ala Ala Gly Ser Val Ile Asn Leu Phe Asp Glu Pro Arg Leu Asn 
        115                 120                 125             


His His Cys Ala Leu Arg Gly Gly Val Glu Ala Gln Asp Cys Ala Arg 
    130                 135                 140                 


Leu Leu Gln Asp Phe Phe Arg Glu Arg Arg Arg Gln Ile Gln Ala Leu 
145                 150                 155                 160 


Gln Gln Lys Pro Ala Ile Pro Ala Asp Arg Ala Asp Pro Asp Glu Gln 
                165                 170                 175     


His Ala Ser Leu His Ala Glu Gly Pro Ala Pro Leu Ala Gly Ile Asp 
            180                 185                 190         


Val Arg His Leu Asp Glu Pro Asp Ala Ala Ala Thr Ser Asn Pro Glu 
        195                 200                 205             


Ser Pro 
    210 


<210> 394
<211> 159
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-12 deaminase

<400> 394
Met Glu Ile Lys Ala Arg Met Arg Asp Ile Met Thr Asp Asp Glu Arg 
1               5                   10                  15      


Trp Met Ala Lys Ala Leu Arg Leu Ala Lys Glu Gly Ala Gln Ala Gly 
            20                  25                  30          


Glu Val Pro Val Gly Ala Ile Leu Val Phe Ser Gly Glu Gln Leu Ala 
        35                  40                  45              


Ala Ala Tyr Asn Ser Pro Ile Arg Leu Arg Asp Ala Thr Ala His Ala 
    50                  55                  60                  


Glu Ile Leu Ala Leu Arg Leu Gly Gly Glu Arg Leu Gln Asn Tyr Arg 
65                  70                  75                  80  


Leu Thr Gly Cys Thr Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys 
                85                  90                  95      


Ala Gly Ala Leu Val Gln Ala Arg Ile Ala Arg Leu Val Phe Gly Ala 
            100                 105                 110         


Arg Asp Ser Arg Val Gly Ala Ala Gly Ser Ala Leu Asp Leu Ala Arg 
        115                 120                 125             


Glu Pro Arg Phe Asn His Gln Val Thr Val Val Gly Gly Val Leu Ala 
    130                 135                 140                 


Ser Glu Cys Glu Gln Val Leu Arg Glu Phe Phe Arg Ala Arg Arg 
145                 150                 155                 


<210> 395
<211> 205
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-13 deaminase

<400> 395
Met Pro Glu Pro Phe Gln Gly Gly Asp Pro Ala Asp Pro Ala Arg Ala 
1               5                   10                  15      


Ala Ala Glu Ala Gln Asp Leu Ala Asp Arg Ala Phe Met Arg Leu Ala 
            20                  25                  30          


Leu Asp Gln Ala His Asn Ala Trp Leu Leu Gly Glu Val Pro Val Gly 
        35                  40                  45              


Ala Val Ile Val His Glu Gly Arg Val Leu Ala Thr Gly Tyr Asn Arg 
    50                  55                  60                  


Pro Ile Gly Asp Ser Asp Pro Thr Ala His Ala Glu Ile Val Ala Leu 
65                  70                  75                  80  


Arg Gln Ala Ala Ser Leu Leu Gly Asn Tyr Arg Leu Pro Gly Cys Thr 
                85                  90                  95      


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Met Ala Leu Leu 
            100                 105                 110         


His Ala Arg Ile Glu Arg Val Val Phe Gly Ala Ser Asp Pro Lys Thr 
        115                 120                 125             


Gly Ala Ala Gly Ser Val Leu Asp Leu Phe Ala Gln Pro Leu Leu Asn 
    130                 135                 140                 


His His Ala Ser Leu Arg Ala Gly Val Glu Ala Gln Ala Cys Gly Ala 
145                 150                 155                 160 


Leu Leu Gln Glu Phe Phe Arg Glu Arg Arg Ala Arg Ala Arg Ala Gln 
                165                 170                 175     


Gln Gln Leu Pro Ala His Gly Gly Gly Ile Asp Val Arg His Leu Asp 
            180                 185                 190         


Ala Pro Glu Pro Gly Asp Glu Ser Asp Pro Phe Asp Glu 
        195                 200                 205 


<210> 396
<211> 159
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-14 deaminase

<400> 396
Met Gly Ser Asp Gln Asp Phe Met Arg Leu Ala Leu Leu Glu Ala Glu 
1               5                   10                  15      


Lys Ala Gly Ala Ile Gly Glu Val Pro Val Gly Ala Val Ile Val Lys 
            20                  25                  30          


Asp Gly Lys Val Val Ala Arg Gly His Asn Gln Pro Val Ser Gly His 
        35                  40                  45              


Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Glu Ala Gly Gln 
    50                  55                  60                  


Val Leu Gly Asn Tyr Arg Leu Pro Ala Cys Asp Leu Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Ala Met Cys Ile Gly Ala Met Ile His Ala Arg Ile Arg 
                85                  90                  95      


Arg Val Ile Phe Gly Ala His Asp Pro Lys Thr Gly Ala Ala Gly Ser 
            100                 105                 110         


Val Ile Asn Leu Phe Glu Glu Lys Thr Leu Asn His His Ala Thr Val 
        115                 120                 125             


Thr Gly Gly Ile Leu Ala Ser Asp Cys Ala Gln Leu Leu Lys Ala Phe 
    130                 135                 140                 


Phe Ala Met Arg Arg Lys Glu Ala Lys Thr Pro Ser Leu Ser Gly 
145                 150                 155                 


<210> 397
<211> 168
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-15 deaminase

<400> 397
Met Glu Pro Met Glu Glu His Arg His Phe Met Gly Leu Ala Ile Glu 
1               5                   10                  15      


Glu Ala Ala Arg Ala Tyr Glu Leu Asn Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Leu Val Gly Ser Ala Gly Glu Ile Leu Ser Arg Ala Ser Asn Ala Pro 
        35                  40                  45              


Ile Arg Ser Ala Asp Pro Ser Ala His Ala Glu Met Ile Ala Leu Arg 
    50                  55                  60                  


Gln Ala Ala Arg Ala Ser Gly Asn Tyr Arg Leu Pro Gly Ser Val Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Val Gly Ala Met Leu His 
                85                  90                  95      


Ala Arg Ile Ala Thr Leu Val Phe Gly Ala Arg Asp Pro Arg Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Val Val Asp Leu Thr Ser Val Pro Ala Phe Asn His 
        115                 120                 125             


Tyr Val Arg Val Ile Ala Gly Val Arg Ala Glu Glu Cys Ser Glu Leu 
    130                 135                 140                 


Leu Arg Arg Phe Phe Arg Glu Arg Arg Arg Glu Gly Lys Ser Gly Tyr 
145                 150                 155                 160 


Gly Gly Glu Val Pro Lys Arg Pro 
                165             


<210> 398
<211> 153
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-16 deaminase

<400> 398
Met Ala Asp Glu Asp Ile Lys Phe Met Arg Leu Ala Leu Glu Leu Ala 
1               5                   10                  15      


Lys Lys Ala Glu Phe Ile Asp Glu Val Pro Val Gly Ala Leu Ile Ile 
            20                  25                  30          


Lys Asp Gly Glu Ile Ile Gly Thr Gly Met Asn Ser Cys Ile Ser Asp 
        35                  40                  45              


His Asp Pro Thr Ser His Ala Glu Ile Lys Ala Ile Arg Asp Ala Ala 
    50                  55                  60                  


Asn Thr Ile Lys Asn Tyr Arg Leu Asn Gly Cys Ser Ile Tyr Val Thr 
65                  70                  75                  80  


Leu Glu Pro Cys Ala Met Cys Val Gly Ala Ile Gln His Ala Arg Ile 
                85                  90                  95      


Glu Lys Ile Ile Tyr Gly Ala Asn Asp Pro Lys Thr Gly Ala Cys Gly 
            100                 105                 110         


Ser Val Ile Asp Leu Met Ser Ile Lys Glu Ile Asn His His Thr Glu 
        115                 120                 125             


Ala Ile Gly Gly Val Leu Gln Lys Glu Cys Gly Gln Ile Leu Lys Asp 
    130                 135                 140                 


Phe Phe Leu Ser Lys Arg Lys Lys Pro 
145                 150             


<210> 399
<211> 148
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-17 deaminase

<400> 399
Met Gln Arg Ala Leu Gly Arg Ala Gly Asp Ala Arg Ala Gln Gly Glu 
1               5                   10                  15      


Val Pro Val Gly Ala Val Val Val Cys Ala Gly Glu Val Val Gly Glu 
            20                  25                  30          


Gly Cys Asn Arg Asn Ile Ser Leu Asp Asp Pro Ser Ala His Ala Glu 
        35                  40                  45              


Ile Leu Ala Leu Arg Ala Ala Gly Gln Arg Leu Gly Asn Tyr Arg Leu 
    50                  55                  60                  


Pro Gly Cys Thr Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Val 
65                  70                  75                  80  


Gly Ala Ala Ile His Ser Arg Leu Asp Gly Val Val Phe Gly Ala Thr 
                85                  90                  95      


Asp Pro Lys Thr Gly Ala Leu Gly Gly Ala Phe Asp Leu Pro Ala Ser 
            100                 105                 110         


His Ala His Asn His Val Leu Ser Val Arg Gly Gly Leu Leu Ala Asp 
        115                 120                 125             


Glu Cys Gly Ala Leu Leu Lys Ala Phe Phe Arg Asp Arg Arg Lys Ala 
    130                 135                 140                 


Pro Arg Ala Pro 
145             


<210> 400
<211> 191
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-18 deaminase

<400> 400
Met Gln Lys Lys Val Asp Asp Phe Leu Lys Ile Val Glu Pro Pro Asp 
1               5                   10                  15      


Ala Glu Arg Arg Thr Lys Gly Lys Leu Leu Gln Gln Arg Ala Val Asp 
            20                  25                  30          


Glu Gln Trp Met Gly Leu Ala Leu Gln Arg Gly Glu Glu Ala Gly Arg 
        35                  40                  45              


Gln Gly Glu Val Pro Val Gly Ala Val Val Val Gly Lys Asp Gly Phe 
    50                  55                  60                  


Leu Thr Ala Ala Gly Asn Ser Pro Ile Gly Leu Ala Asp Pro Ser Ala 
65                  70                  75                  80  


His Ala Glu Met Leu Ala Ile Arg Ala Ala Ala Lys Leu Leu Gly Asn 
                85                  90                  95      


Tyr Arg Leu Pro Glu Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Ile 
            100                 105                 110         


Met Cys Met Gly Ala Leu Ile Gln Ala Arg Ile Ser Arg Leu Val Phe 
        115                 120                 125             


Gly Ala Ala Asp Pro Lys Thr Gly Ala Ala Ile Ser Val Tyr Ala Ile 
    130                 135                 140                 


Gly Thr Asp Gln Arg Leu Asn His Thr Val Ala Val Thr Gly Gly Ile 
145                 150                 155                 160 


Leu Ala Glu Ala Cys Ala Gln Leu Leu Lys Asn Phe Phe Lys Lys Arg 
                165                 170                 175     


Arg Gly His Ser Pro Val Ile Asn Ser Pro Phe Leu Lys Thr Pro 
            180                 185                 190     


<210> 401
<211> 144
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-19 deaminase

<400> 401
Met Arg Glu Ala Tyr Ala Leu Ala Leu Ile Ala Tyr Glu Gln Gly Glu 
1               5                   10                  15      


Val Pro Val Gly Ala Val Leu Val Gly Glu Asp Asp Glu Ile Leu Gly 
            20                  25                  30          


Arg Gly Trp Asn Gln Val Ile Gln Arg His Asp Pro Met Ala His Ala 
        35                  40                  45              


Glu Cys Val Ala Ile Gln Glu Ala Ala Ala Gln Leu Arg Asn Tyr Arg 
    50                  55                  60                  


Leu Asn Gly Ser Thr Leu Tyr Val Thr Leu Glu Pro Cys Cys Met Cys 
65                  70                  75                  80  


Ala Gly Ala Met Val His Ala Arg Ile Ala Arg Leu Val Phe Gly Ala 
                85                  90                  95      


Arg Asp Val Arg Ser Gly Ala Ala Gly Ser Ala Tyr Asn Leu Leu Ala 
            100                 105                 110         


Gly Asp Leu Leu Asn His Arg Val Lys Ile Asp Glu Gly Ile Leu Gln 
        115                 120                 125             


Gln Glu Cys Ser His Leu Leu Arg Asn Phe Phe Lys Gln Arg Arg Asp 
    130                 135                 140                 


<210> 402
<211> 195
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-20 deaminase

<400> 402
Met Thr Ser Gln Val Ser Arg Gln Gln Pro Leu Ser Gly Asn Asn Leu 
1               5                   10                  15      


Val His Asn Asp Leu Ile Ala Ser Val Cys Pro Pro Gly Leu Val Pro 
            20                  25                  30          


Ala Gln Gln Asp Glu Phe Trp Met Gln Gln Ala Leu Val Leu Ala Arg 
        35                  40                  45              


Gln Ala Glu Met Met Gly Glu Val Pro Val Gly Ala Val Val Val Lys 
    50                  55                  60                  


Asn Gln Gln Leu Ile Gly Tyr Gly Tyr Asn Gln Ser Ile Thr Leu Asn 
65                  70                  75                  80  


Asp Pro Ser Ala His Ala Glu Val Leu Ala Leu Arg Met Ala Gly Gln 
                85                  90                  95      


Thr Leu Thr Asn Tyr Arg Leu Pro Asp Thr Thr Leu Tyr Val Thr Leu 
            100                 105                 110         


Glu Pro Cys Ala Met Cys Ala Thr Thr Met Val His Ala Arg Val Ala 
        115                 120                 125             


Arg Leu Val Tyr Gly Ala Ala Asp Leu Lys Thr Gly Ala Ala Gly Ser 
    130                 135                 140                 


Val Leu Asn Leu Thr Asn Tyr Thr Ala Phe Asn His Gln Leu Ser Cys 
145                 150                 155                 160 


Val Ser Gly Val Leu Gln Arg Asp Cys Ser Tyr Leu Leu Ser Asn Phe 
                165                 170                 175     


Phe Lys Leu Arg Arg Thr Gln His Lys Ala Lys Lys Val Ser Gly Thr 
            180                 185                 190         


Phe Ile Arg 
        195 


<210> 403
<211> 162
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-21 deaminase

<400> 403
Met Asp His Glu Arg Tyr Met Arg Arg Ala Ile Glu Leu Ala Gln Gln 
1               5                   10                  15      


Ala Glu Ser Leu Gly Glu Val Pro Val Gly Ala Val Ile Val His Asp 
            20                  25                  30          


His Gln Ile Ile Gly Glu Gly Phe Asn Glu Val Ile Arg Arg Asn Asp 
        35                  40                  45              


Pro Ser Ala His Ala Glu Ala Gln Ala Ile Arg Ala Ala Gly Gln His 
    50                  55                  60                  


Leu Ala Asn Tyr Arg Leu Val Asn Thr Thr Leu Tyr Val Ser Leu Glu 
65                  70                  75                  80  


Pro Cys Ala Met Cys Ala Gly Leu Ile Val His Ala Arg Val Lys Thr 
                85                  90                  95      


Val Val Phe Ala Thr Pro Asp Pro Arg Thr Gly Ala Thr Gly Thr Ala 
            100                 105                 110         


Ile Gln Val Leu Asn His Pro Ser Met Asn His His Val Glu Val Val 
        115                 120                 125             


Ser Gly Val Leu Gln Glu Gln Ser Ala Thr Ile Leu Gln Asn Phe Phe 
    130                 135                 140                 


Arg Lys Lys Arg Glu Leu Ala Lys Met Lys Lys Phe His Glu Lys Arg 
145                 150                 155                 160 


Val Asn 
        


<210> 404
<211> 166
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-22 deaminase

<400> 404
Met Ser Asp Glu His Phe Met Arg Leu Ala Leu Ala Gln Ala Ala Leu 
1               5                   10                  15      


Ala Glu Ala Glu Gly Glu Ile Pro Val Gly Ala Val Val Val Leu Asn 
            20                  25                  30          


Gly Glu Val Val Gly Ser Gly Tyr Asn Ser Pro Ile Gly Arg His Asp 
        35                  40                  45              


Pro Thr Ala His Ala Glu Ile Arg Ala Leu Arg Ala Ala Ala Ala Thr 
    50                  55                  60                  


Leu Gly Asn Tyr Arg Leu Val Gly Cys Glu Leu Phe Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Leu Met Cys Ala Gly Ala Ile Gln His Ala Arg Ile Ala Arg 
                85                  90                  95      


Leu Val Tyr Gly Ala Ala Asp Tyr Lys Thr Gly Val Cys Gly Ser Thr 
            100                 105                 110         


Leu Asp Val Phe Gly Glu Leu Pro Gly Ser Pro Val Pro Arg Leu Asn 
        115                 120                 125             


His His Thr Gln Val Thr Gly Gly Val Leu Gly Asp Glu Cys Ser Arg 
    130                 135                 140                 


Gln Leu Ser Ala Phe Phe Ala Arg Arg Arg Ala Glu Lys Asn Ala Ala 
145                 150                 155                 160 


Lys Asn His Val Phe Gln 
                165     


<210> 405
<211> 161
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-23 deaminase

<400> 405
Met Asp Ser Leu Val Glu Arg His Glu His Tyr Met Met Leu Ala Leu 
1               5                   10                  15      


Arg Leu Ala Glu Lys Gly Ala Asp Arg Gly Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Val Val Ser Pro Lys Gly Gln Ile Leu Ser Leu Ala His Asn Glu 
        35                  40                  45              


Pro Ile Asp Leu His Asp Pro Thr Gly His Ala Glu Ile Ile Ala Ile 
    50                  55                  60                  


Arg Lys Ala Ala Gln Arg Ile Gly Asn Tyr Arg Leu Val Gly Cys Ser 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys Tyr Gly Ala Ile Ile 
                85                  90                  95      


His Ala Arg Ile Lys Arg Leu Phe Phe Gly Ala Tyr Asp Pro Lys Ala 
            100                 105                 110         


Gly Ala Gly Thr Val Phe Gly Leu Phe Ser Ser Pro Phe Phe Asn His 
        115                 120                 125             


Gln Pro Glu Val Arg Gly Gly Ile Leu Ala Glu Leu Cys Glu Lys Val 
    130                 135                 140                 


Leu Lys Glu Phe Phe Glu Lys Leu Arg Ser Ser Lys Cys Cys Ser Ser 
145                 150                 155                 160 


Gly 
    


<210> 406
<211> 152
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-24 deaminase

<400> 406
Met Thr Glu Asp Glu Arg Trp Met Glu Glu Ala Leu Ala Leu Ala Arg 
1               5                   10                  15      


Glu Ala Ser Asn Asp Gly Glu Ala Pro Val Gly Ala Val Val Val His 
            20                  25                  30          


Ala Gly Glu Ile Ile Gly Arg Gly Arg Asn Arg Ile Glu Gly Leu Ala 
        35                  40                  45              


Asp Pro Thr Ala His Ala Glu Leu Leu Ala Leu Arg Gln Ala Ser Met 
    50                  55                  60                  


Asn Leu Gly Arg Trp Arg Leu Thr Gly Cys Ala Leu Phe Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Ala Met Cys Leu Gly Ala Cys Tyr Ala Ala Arg Val Asp 
                85                  90                  95      


Arg Leu Val Tyr Gly Ala Thr Ser Pro Lys Phe Gly Ala Ala Gly Ser 
            100                 105                 110         


Ser Val Thr Leu Ala Gly Val Glu Arg Leu Asn His Arg Leu Glu Val 
        115                 120                 125             


Val Ser Gly Val Leu Glu Lys Glu Cys Ala Arg Leu Leu Ala Lys Phe 
    130                 135                 140                 


Phe Arg Gly Leu Arg Gly Lys Gly 
145                 150         


<210> 407
<211> 168
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-25 deaminase

<400> 407
Met Asp Asp Gln His Trp Met Gly Arg Ala Leu Ala Leu Ala Glu Arg 
1               5                   10                  15      


Ala Ala Glu Gln Gly Glu Val Pro Gly Gly Ala Val Leu Val Ile Glu 
            20                  25                  30          


Asn Glu Ile Val Gly Glu Gly Trp Ser Arg Pro Ile Ala Thr Gln Asp 
        35                  40                  45              


Pro Ser Ala His Ala Glu Ile Leu Ala Leu Arg Asp Ala Gly Leu Arg 
    50                  55                  60                  


Arg Gly Asn Tyr Arg Leu Ser Gly Ser Val Leu Tyr Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Val Met Cys Ala Gly Ala Ile Val His Ala Arg Val Asp Thr 
                85                  90                  95      


Leu Val Phe Gly Ala Ala Asp Pro Lys Ala Gly Ala Cys Gly Ser Val 
            100                 105                 110         


Phe Asp Leu Leu Pro Ser Asp Gly Arg Phe Asn His His Thr Gln Cys 
        115                 120                 125             


Arg Gly Pro Ile Leu Ala Asp Ala Cys Gly Thr Ile Leu Arg Ala Phe 
    130                 135                 140                 


Phe Ala Asn Lys Arg Ala Ser Pro Arg Ala Arg Ala Asn Ala Ser Ala 
145                 150                 155                 160 


Ala His Glu Arg Pro Glu Asn Asp 
                165             


<210> 408
<211> 173
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-26 deaminase

<400> 408
Met Ser Glu Ser Gln Phe Asp Glu Thr Gln Tyr Arg Trp Met Asp Arg 
1               5                   10                  15      


Ala Leu Glu Leu Ala Gly Gln Ala Ala Asn Leu Gly Glu Val Pro Val 
            20                  25                  30          


Gly Ala Val Ile Val Ser Ala Glu Gly Glu Leu Leu Gly Glu Gly Phe 
        35                  40                  45              


Asn Gln Pro Ile Lys Val Gln Asp Pro Thr Ala His Ala Glu Ile Met 
    50                  55                  60                  


Ala Ile Arg Gln Ala Cys Arg Lys Thr Gly Asn Tyr Arg Leu Pro Gly 
65                  70                  75                  80  


Ala Val Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala 
                85                  90                  95      


Ile Val His Ala Arg Leu Ala Arg Val Val Phe Ala Thr Cys Glu Pro 
            100                 105                 110         


Lys Ala Gly Ala Val Glu Ser Thr Leu Arg Phe Phe Asp Leu Pro Gln 
        115                 120                 125             


Leu Asn His Arg Val Glu Tyr Thr Gly Gly Leu Arg Arg Ser Glu Ala 
    130                 135                 140                 


Ser Ser Leu Leu Ser His Phe Phe Gln Ser Arg Arg Glu Glu Lys Ala 
145                 150                 155                 160 


Leu Leu Lys Lys Asn Ser Arg Gln Gln Gln Ser Asp Cys 
                165                 170             


<210> 409
<211> 164
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-27 deaminase

<400> 409
Met Arg Glu Glu Lys Asp Leu Ile Phe Met Lys Lys Ala Leu His Leu 
1               5                   10                  15      


Ala Glu Glu Ala Trp Arg Gln Gly Glu Val Pro Val Gly Ala Val Val 
            20                  25                  30          


Val Asp Gly Glu Gly Gln Ile Ile Gly Gln Gly Tyr Asn Gln Ser Ile 
        35                  40                  45              


Ala Leu Leu Asp Pro Thr Ala His Ala Glu Ile Val Ala Leu Arg Gln 
    50                  55                  60                  


Ala Ala Lys Lys Lys Lys Asn Tyr Arg Leu Ser Gly Ser Thr Leu Tyr 
65                  70                  75                  80  


Val Thr Leu Glu Pro Cys Pro Met Cys Leu Gly Ala Met Val Gln Ala 
                85                  90                  95      


Arg Ile Asp Arg Leu Val Phe Gly Ala Tyr Asp Pro Lys Asn Gly Ala 
            100                 105                 110         


Val Ser Ser Ile Met Asn Phe Pro Leu Asp Lys Met Asn His Arg Phe 
        115                 120                 125             


Glu Ile Glu Gly Gly Leu Leu Ser Gln Glu Cys Ala Thr Ile Leu Lys 
    130                 135                 140                 


Glu Phe Phe Gln Ser Arg Arg Gly Glu Thr Asn Asn Lys Lys Glu Thr 
145                 150                 155                 160 


Ala Lys Glu Glu 
                


<210> 410
<211> 153
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-28 deaminase

<400> 410
Met Asp Thr Asp Glu Gln Phe Met Gln His Ala Ile Gln Leu Ala His 
1               5                   10                  15      


Lys Ala Gln Ser Gln Gly Glu Val Pro Val Gly Ala Ile Leu Val Lys 
            20                  25                  30          


Asp Asn Lys Ile Ile Gly Lys Gly Trp Asn Gln Pro Ile Thr Arg His 
        35                  40                  45              


Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile Arg His Ala Gly Gln 
    50                  55                  60                  


Ala Leu Lys Asn Tyr Arg Leu Pro Asp Thr Thr Leu Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Asn Met Cys Ala Gly Met Ile Ile His Ala Arg Ile Lys 
                85                  90                  95      


Arg Leu Val Phe Ala Ala Tyr Asp Pro Lys Thr Gly Ala Ala Gly Ser 
            100                 105                 110         


Val Phe Asp Thr Leu Thr Asp Pro Arg Asn Asn His Thr Val Glu Ile 
        115                 120                 125             


Glu Gly Gly Val Leu Glu Glu Pro Cys Ala Glu Met Leu Arg His Phe 
    130                 135                 140                 


Phe Arg Gln Arg Arg Lys Lys Thr Pro 
145                 150             


<210> 411
<211> 158
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-29 deaminase

<400> 411
Met Ser Glu Leu Glu Asn Asn Asn Pro Gln His Ile Lys Trp Met Arg 
1               5                   10                  15      


Glu Ala Ile Lys Leu Ala Gln Lys Ala Gln Glu Cys Gly Glu Val Pro 
            20                  25                  30          


Val Gly Ala Leu Ile Thr Lys Asn Asp Thr Leu Ile Ser Ser Ala Tyr 
        35                  40                  45              


Asn Gln Pro Ile Thr Lys His Asp Pro Thr Ala His Ala Glu Ile Gln 
    50                  55                  60                  


Ala Leu Gln Gln Ala Gly Lys Ile Leu Gln Asn Tyr Arg Leu Asn Asp 
65                  70                  75                  80  


Thr Val Leu Tyr Ile Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala 
                85                  90                  95      


Ile Val His Ala Arg Ile Gly Thr Val Val Phe Gly Ala Tyr Asp His 
            100                 105                 110         


Lys Thr Gly Val Ala Gly Ser Ala Gly Asn Ile Phe Thr Ala Glu Phe 
        115                 120                 125             


Ser Asn His Lys Pro Lys Ile Ile Gly Gly Thr Leu Glu Lys Glu Cys 
    130                 135                 140                 


Ser Leu Ile Ile Arg Asn Phe Phe Gln Ala Arg Arg Gln Lys 
145                 150                 155             


<210> 412
<211> 182
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-30 deaminase

<400> 412
Met Pro Asn Phe Leu Lys Gln Leu Gln Gln Ile Ile Ala Phe Glu Thr 
1               5                   10                  15      


Ser Cys Ala Ala Asp Trp Ser Glu Gln Gln Lys His Val Phe Trp Met 
            20                  25                  30          


Gln Gln Ala Leu Lys Val Ala Ala Tyr Gly Glu Gln Leu Gly Glu Val 
        35                  40                  45              


Pro Val Gly Ala Val Ala Val Val Glu Asn Gln Leu Ile Ala Val Gly 
    50                  55                  60                  


Trp Asn Ala Ser Ile Ser Lys His Asp Pro Thr Ala His Ala Glu Val 
65                  70                  75                  80  


Met Ala Leu Arg Ala Ala Gly Thr Val Leu Lys Asn Tyr Arg Gln Leu 
                85                  90                  95      


Gln Leu Thr Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly 
            100                 105                 110         


Ala Met Val His Ala Arg Ile Ala Gln Leu Val Tyr Gly Ala Ser Asp 
        115                 120                 125             


Pro Lys Thr Gly Ala Val Asp Ser Val Phe Asn Leu Leu Arg His Pro 
    130                 135                 140                 


Asn Leu Asn His Gln Ala Pro Val Leu Gly Gly Val Leu Ala Thr Glu 
145                 150                 155                 160 


Cys Arg Thr Gln Leu Gln Asp Phe Phe Arg Arg Arg Arg Gln Glu Lys 
                165                 170                 175     


Lys Gln Ala Gln Gln Lys 
            180         


<210> 413
<211> 167
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-31 deaminase

<400> 413
Met Glu Ser Val Asp Glu Gln Trp Met Arg Tyr Ala Ile Arg Leu Ala 
1               5                   10                  15      


Gln Arg Ala Glu Glu Gln Gly Glu Val Pro Val Gly Ala Val Leu Val 
            20                  25                  30          


Lys Gly Asp Arg Cys Leu Ala Glu Gly Trp Asn Val Pro Val Gln Arg 
        35                  40                  45              


His Asp Pro Thr Ala His Ala Glu Met Val Ala Ile Arg Asp Ala Gly 
    50                  55                  60                  


Ile Ala Leu Glu Asn Tyr Arg Leu Ile Asp Ser Thr Leu Tyr Val Thr 
65                  70                  75                  80  


Leu Glu Pro Cys Val Met Cys Met Gly Ala Ile Ala His Ala Arg Ile 
                85                  90                  95      


Lys Arg Leu Val Phe Gly Ala Phe Asp Pro Lys Arg Gly Ala Val Cys 
            100                 105                 110         


Asn Ala Leu Ser Leu Thr Asp Ala Ser Phe Leu Asn His Lys Val Val 
        115                 120                 125             


Trp Asp Gly Gly Val Phe Glu Gln Glu Cys Ser Glu Leu Leu Ile Asp 
    130                 135                 140                 


Phe Phe Lys Ala Arg Arg Lys Val Thr Lys Pro Glu Val Val Val Thr 
145                 150                 155                 160 


Asn Pro Gln Arg His Gly Met 
                165         


<210> 414
<211> 160
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-32 deaminase

<400> 414
Met Tyr Asn Gln Gln Ala Ile Asp Lys His Phe Met Asn Cys Ala Leu 
1               5                   10                  15      


Glu Leu Ala Thr Phe Ala Ala Asp Ser Gly Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Ile Val Arg Lys Asn Thr Ile Val Ala Lys Gly Ser Asn Ser Pro 
        35                  40                  45              


Ile Ala Asp His Asp Pro Thr Ala His Ala Glu Ile Ile Ala Ile Arg 
    50                  55                  60                  


Lys Ala Thr Ala Gln Cys Ala Asn Tyr Arg Thr Pro Asp Thr Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Ile Met Cys Met Gly Ala Ile Ile Gln 
                85                  90                  95      


Ala Arg Ile Gly Arg Leu Val Phe Gly Ala Tyr Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Cys Tyr Ser Ile Gly Gln Asp Asn Leu Leu Asn His 
        115                 120                 125             


Ser Leu Glu Ile Ala Gly Gly Ile Asn Glu Gln Gln Cys Ala Ile Leu 
    130                 135                 140                 


Leu Lys Lys Phe Phe Lys Ile Arg Arg Lys Lys Gly Ser Lys Asn Asn 
145                 150                 155                 160 


<210> 415
<211> 150
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-33 deaminase

<400> 415
Met Ser Asp Leu Glu Trp Met Arg Arg Ala Ile Thr Leu Ala Gln Lys 
1               5                   10                  15      


Ala Glu Gln Gln Gly Glu Val Pro Val Gly Ala Leu Leu Val Arg Glu 
            20                  25                  30          


Gly Val Cys Val Gly Glu Gly Trp Asn Gln Pro Ile Leu Arg His Asp 
        35                  40                  45              


Pro Ser Ala His Ala Glu Ile Asn Ala Leu Arg Asp Ala Gly Gln Ala 
    50                  55                  60                  


Thr Glu Asn Tyr Arg Leu Ile Asp Thr Val Leu Tyr Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Val Met Cys Met Gly Ala Ile Ala Asn Ala Arg Val Arg Arg 
                85                  90                  95      


Leu Val Phe Gly Ala Ser Asp Pro Ala Arg Gly Ala Ala Gly Ser Ile 
            100                 105                 110         


Leu Ser Leu Ser Asn Ala Ser Phe Leu Asn His His Ile Glu Thr Glu 
        115                 120                 125             


Gly Gly Leu Leu Ala Glu Glu Cys Ser Glu Leu Leu Arg Ser Phe Phe 
    130                 135                 140                 


Ala Arg Arg Arg Arg Lys 
145                 150 


<210> 416
<211> 155
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-34 deaminase

<400> 416
Met Ser Pro Val Val Ala Glu Ser Asp Arg Ala Trp Met Arg His Ala 
1               5                   10                  15      


Leu Glu Leu Ala Asn Arg Ala Gly Glu Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Val Met Val Lys Asp Gly Gln Leu Val Gly Glu Gly Trp Asn Arg 
        35                  40                  45              


Val Ile Gly Leu Asn Asp Pro Ser Ala His Ala Glu Ile Leu Ala Leu 
    50                  55                  60                  


Arg Glu Ala Gly Gln Arg Leu Gly Asn Tyr Arg Leu Pro Gly Cys Ser 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Met Leu 
                85                  90                  95      


His Ala Arg Leu Glu Arg Val Tyr Phe Gly Thr Gly Asp Pro Lys Thr 
            100                 105                 110         


Gly Ala Leu Gly Gly Thr Phe Asp Leu Phe Ala Asp Tyr Pro His Asn 
        115                 120                 125             


His Arg Ile Glu Thr Gln Gly Gly Leu Leu Glu Ile Glu Cys Ser Ser 
    130                 135                 140                 


Leu Leu Lys Glu Phe Phe Arg Ala Arg Arg Leu 
145                 150                 155 


<210> 417
<211> 194
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-35 deaminase

<400> 417
Met Gly Leu Asn Gly Leu Thr His His Ser Pro Phe Ile Pro Ile Ser 
1               5                   10                  15      


Glu Asn Ile Asp Thr Ser Asp Lys Leu Ala Leu Gln Ile Met Ser Leu 
            20                  25                  30          


Lys Leu Ser Asn Pro Asp Glu Lys Trp Met Arg His Ala Gln Ser Leu 
        35                  40                  45              


Ala Glu His Ala Ala Ala Gln Gly Glu Ile Pro Val Gly Ala Val Ile 
    50                  55                  60                  


Val Gln Ala Gly Glu Cys Ile Ala Glu Gly Trp Asn Gln Pro Ile Ala 
65                  70                  75                  80  


Asn Asn Asp Pro Thr Ala His Ala Glu Ile Ile Ala Leu Arg Lys Ala 
                85                  90                  95      


Ala Gln Tyr Leu Asn Asn Tyr Arg Leu Val Asn Thr Leu Leu Tyr Val 
            100                 105                 110         


Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Ile Ile Gln Ala Arg 
        115                 120                 125             


Val Lys Arg Val Ile Phe Gly Ala Tyr Asp Pro Lys Ala Gly Ala Ala 
    130                 135                 140                 


Gly Ser Arg Phe Asp Ile Leu Arg Asp Thr Arg His Asn Tyr Gln Val 
145                 150                 155                 160 


Glu Cys Ile Ser Gly Val Leu Ala Lys Glu Cys Ser Asp Tyr Leu Ser 
                165                 170                 175     


Arg Phe Phe Lys Asn Arg Arg Thr Arg Ala Lys Pro Ser Arg Phe Pro 
            180                 185                 190         


Lys Pro 
        


<210> 418
<211> 174
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-36 deaminase

<400> 418
Met Gln Asp Asp Thr Val Pro Glu Glu Ile Asp His Glu Arg Trp Met 
1               5                   10                  15      


Arg Gln Ala Leu Asn Glu Ala Met Asp Ala Ala Glu Glu Gly Glu Val 
            20                  25                  30          


Pro Val Gly Ala Val Val Val Tyr Glu Asn Arg Ile Ile Gly Arg Gly 
        35                  40                  45              


His Asn Arg Thr Val Ala Thr His Asp Pro Ser Ala His Ala Glu Ile 
    50                  55                  60                  


Leu Ala Leu Gly Ala Ala Gly Ala Ala Val Gly Asp Trp Arg Leu Thr 
65                  70                  75                  80  


Gly Val Asp Ile Tyr Val Ser Leu Glu Pro Cys Ala Met Cys Ala Gly 
                85                  90                  95      


Ala Ile Gln Leu Gly Arg Leu Arg Arg Ala Val Phe Gly Pro Arg Asp 
            100                 105                 110         


Pro Lys Phe Gly Gly Cys Gly Ser Val Leu Asp Val Leu Arg Glu Pro 
        115                 120                 125             


Arg Leu Asn His Gln Val Glu Val Ile Glu Gly Val Met Ala Ala Glu 
    130                 135                 140                 


Ser Gln His Leu Leu Lys Glu Phe Phe Arg Arg Leu Arg Ala Lys Asp 
145                 150                 155                 160 


Arg Val Pro Ala Thr Pro Asp Pro Phe Pro Asn Ala Gly Glu 
                165                 170                 


<210> 419
<211> 149
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-37 deaminase

<400> 419
Met Glu Glu Glu Tyr Phe Met Arg Glu Ala Leu Lys Glu Ala Glu Lys 
1               5                   10                  15      


Ala Ala Gln Glu Gly Glu Val Pro Ile Gly Ala Val Val Val Gly Pro 
            20                  25                  30          


Ser Arg Glu Ile Ile Gly Arg Gly Tyr Asn Arg Pro Ile Gly Leu Cys 
        35                  40                  45              


Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Glu Ala Ala Lys 
    50                  55                  60                  


Arg Leu Gly Asn Tyr Arg Leu Leu Asp Cys Glu Ile Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Pro Met Cys Ala Gly Ala Leu Val Tyr Ala Arg Leu Lys 
                85                  90                  95      


Arg Leu Val Phe Gly Ala His Asp Pro Lys Ala Gly Ala Cys Gly Ser 
            100                 105                 110         


Val Tyr Asn Ile Val Gln Asp Lys Arg Leu Asn His Gln Leu Glu Val 
        115                 120                 125             


Ala Gly Gly Leu Leu Ala Glu Glu Cys Arg Ala Leu Leu Gln Ala Phe 
    130                 135                 140                 


Phe Lys Ser Arg Arg 
145                 


<210> 420
<211> 160
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-38 deaminase

<400> 420
Met Phe Glu Phe Ser Asp Asn Lys Ala Glu Phe Phe Met Lys Gln Ala 
1               5                   10                  15      


Ile Leu Gln Ala Ile Arg Ala Trp Lys Lys Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Val Val Val Ser Gly Ala Gly Gln Ile Leu Ala Ser Ala His Asn 
        35                  40                  45              


Cys Pro Ile Ser Leu Asp Asp Pro Thr Ala His Ala Glu Ile Leu Ala 
    50                  55                  60                  


Leu Arg Leu Ala Ala Gly Lys Val Gly Asn Tyr Arg Leu Thr Asp Ser 
65                  70                  75                  80  


Trp Leu Ile Ser Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met 
                85                  90                  95      


Val Trp Ala Arg Val Lys Gly Val Val Phe Gly Ala Arg Asp Pro Val 
            100                 105                 110         


Ser Gly Ala Phe Gly Ser Val Met Asp Ile Asn Glu Val Pro Gly Leu 
        115                 120                 125             


Asn His Arg Ile Glu Val Thr Glu Gly Val Leu Ala Ala Glu Cys Ser 
    130                 135                 140                 


Gln Leu Leu Lys Asp Phe Phe Lys Arg Arg Arg Gln Ser Ser Ala Gly 
145                 150                 155                 160 


<210> 421
<211> 148
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-39 deaminase

<400> 421
Met Thr Asp Glu Asn Trp Met Gln Arg Ala Leu Gln Leu Ala Ala Gln 
1               5                   10                  15      


Ala Glu Ala Val Gly Glu Val Pro Val Gly Ala Val Ile Val Lys Asn 
            20                  25                  30          


Asp Thr Leu Val Ala Glu Gly Phe Asn Gln Pro Ile Gly Ala His Asp 
        35                  40                  45              


Ala Thr Ala His Ala Glu Ile Val Ala Leu Arg Arg Ala Gly Glu Ala 
    50                  55                  60                  


Leu Gly Asn Tyr Arg Leu Val Asp Thr Thr Leu Tyr Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Ser Met Cys Val Gly Ala Met Ile His Ala Arg Val Ala Lys 
                85                  90                  95      


Val Val Phe Gly Ala Ser Asp Pro Arg Thr Gly Ala Leu Gly Gly Ala 
            100                 105                 110         


Phe Asp Leu Gln Gln Ala Gly Arg His Asn His Arg Phe Asp Val Val 
        115                 120                 125             


Ser Gly Val Met Ala Asp Ser Ser Arg Gln Leu Leu Gln Asn Phe Phe 
    130                 135                 140                 


Arg Val Arg Arg 
145             


<210> 422
<211> 149
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-40 deaminase

<400> 422
Met Thr Glu Asp Glu Lys Trp Met Asn Leu Ala Ile Glu Gln Ala Ile 
1               5                   10                  15      


Lys Ala Gln Asp Glu Asp Glu Val Pro Val Gly Ala Val Leu Val Lys 
            20                  25                  30          


Asn Gly Leu Leu Leu Ser Gln Ala His Asn Gln Pro Ile Ser Thr Asn 
        35                  40                  45              


Asp Ala Thr Ala His Ala Glu Ile Gln Ala Leu Arg Lys Ala Gly Ile 
    50                  55                  60                  


Lys Leu Gln Asn Tyr Arg Leu Thr Asp Ser Thr Leu Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Ala Met Cys Leu Gly Ala Met Met His Ala Arg Ile Ala 
                85                  90                  95      


Arg Ile Val Tyr Gly Ala Asn Asp Leu Lys Thr Gly Ala Cys Gly Ser 
            100                 105                 110         


Ser Glu Asn Leu Leu Asn Ala Asn Cys Phe Asn His Lys Ile His Leu 
        115                 120                 125             


Ile Ser Gly Val Leu Glu Asn Glu Cys Lys Gln Leu Leu Lys Lys Phe 
    130                 135                 140                 


Phe Asn Ser Arg Arg 
145                 


<210> 423
<211> 185
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-41 deaminase

<400> 423
Met Asn Gln Val Asn Cys Gly Arg Ile Gly Ser Arg Leu Ile Arg Thr 
1               5                   10                  15      


Gly Gly Val Asn Ile Ser Arg Ala Pro Ile Glu Glu Asp Pro Val Asn 
            20                  25                  30          


Asp Leu Gln Tyr Ala Gln Asp Thr Lys Trp Met Arg Cys Ala Leu Asp 
        35                  40                  45              


Cys Ala Arg Arg Ala Tyr Ser Thr Glu Glu Val Pro Ile Gly Ala Val 
    50                  55                  60                  


Leu Val Arg Asn Glu Gln Ile Ile Gly Glu Gly Phe Asn Gln Thr Ile 
65                  70                  75                  80  


Arg Asn Leu Asp Pro Thr Ser His Ala Glu Ile Met Ala Ile Arg Ser 
                85                  90                  95      


Ala Ala Glu Asn Ile Gly Asn Tyr Arg Leu Ser Asp Thr Thr Leu Tyr 
            100                 105                 110         


Val Thr Ile Glu Pro Cys Met Met Cys Leu Gly Ala Ile Ile His Ala 
        115                 120                 125             


Arg Val Glu Arg Leu Val Phe Gly Ala Gln Glu Pro Lys Ala Gly Val 
    130                 135                 140                 


Leu Lys Ser His Pro Val Phe Asp Gly Asp Phe Leu Asn His Gln Val 
145                 150                 155                 160 


Lys Trp Ser Ser Gly Ile Leu Glu Ser Glu Cys Ser Arg Leu Met Arg 
                165                 170                 175     


Asp Phe Phe Ile His Lys Arg Thr Leu 
            180                 185 


<210> 424
<211> 203
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-42 deaminase

<400> 424
Met Ala Pro Val Pro Leu Lys Gln Pro Leu Ala Gly Glu Ser Phe Asp 
1               5                   10                  15      


Ala His Asp His Ala Met Met Arg Leu Ala Ile Asp Gln Ala Leu Asn 
            20                  25                  30          


Ala Gln Leu His Gly Glu Val Pro Val Gly Ala Val Leu Val Lys Gly 
        35                  40                  45              


Gly Gln Val Ile Ala Thr Gly Tyr Asn His Pro Ile Gly Ser His Asp 
    50                  55                  60                  


Pro Thr Ala His Ala Glu Ile Arg Thr Leu Arg Met Ala Ala Glu Gln 
65                  70                  75                  80  


Leu Gly Asn Tyr Arg Leu Gly Glu Ser Thr Leu Tyr Val Thr Leu Glu 
                85                  90                  95      


Pro Cys Met Met Cys Leu Gly Ala Met Leu His Ala Arg Ile Thr Arg 
            100                 105                 110         


Val Val Phe Gly Ala Ser Asp Pro Lys Thr Gly Val Cys Gly Gly Val 
        115                 120                 125             


Leu Asp Leu Pro Ala Glu Gly Arg Leu Asn His Gln Thr Thr Val Gln 
    130                 135                 140                 


Gly Gly Leu Leu Ala Glu Glu Cys Gly Glu Leu Leu Gln Arg Phe Phe 
145                 150                 155                 160 


Ala Glu Arg Arg Ala Gln Arg Arg Ala Asp Arg Glu Ala Leu Arg Ala 
                165                 170                 175     


Gln Gln Asp Glu Leu Leu Arg Trp Ala Ala Thr Leu Pro Ala Glu Glu 
            180                 185                 190         


Glu Val Gly Thr Leu Arg Leu Asp Glu Glu Thr 
        195                 200             


<210> 425
<211> 150
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-43 deaminase

<400> 425
Met Ala Met Ala Leu Ala Glu Ala Glu Lys Ala Met Gln Ile Gly Glu 
1               5                   10                  15      


Val Pro Ile Gly Ala Val Val Val Gly Pro Asp Gln Gln Leu Leu Gly 
            20                  25                  30          


Thr Gly Tyr Asn Gln Ser Ile Cys Leu Asn Asp Pro Ser Ala His Ala 
        35                  40                  45              


Glu Met Leu Ala Ile Arg Ala Ala Ala Ala Gln Leu Gly Asn Tyr Arg 
    50                  55                  60                  


Leu Arg Asp Cys Thr Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys 
65                  70                  75                  80  


Met Gly Ala Ile Leu His Ser Arg Ile Gln Arg Val Val Phe Ala Ala 
                85                  90                  95      


Ala Asp Pro Lys Thr Gly Ala Cys Gly Ser Val Leu Ser Leu Gln Ser 
            100                 105                 110         


Glu Pro Arg Leu Asn His His Cys Ser Val Thr Gln Gly Val Leu Ala 
        115                 120                 125             


Glu Arg Ala Ala Ser Leu Ile Lys Gly Phe Phe Lys Ala Arg Arg Gln 
    130                 135                 140                 


Val Lys Lys Cys Arg Cys 
145                 150 


<210> 426
<211> 164
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-44 deaminase

<400> 426
Met Leu His Met Val Tyr Tyr Glu Ile Asn Glu Thr Asn Ser Gln Asp 
1               5                   10                  15      


Val Phe Phe Met Arg Arg Ala Leu Thr Leu Ala Gln Lys Ala Ala Ser 
            20                  25                  30          


Ala Asp Glu Val Pro Val Gly Ala Val Ala Ile Ala Asn Asn Glu Ile 
        35                  40                  45              


Ile Gly Glu Gly Phe Asn Cys Pro Ile Gly Thr His Asp Pro Thr Ala 
    50                  55                  60                  


His Ala Glu Ile Met Ala Leu Arg Gln Ala Ala Gln Lys Ser Asn Asn 
65                  70                  75                  80  


Tyr Arg Leu Pro Glu Val Thr Leu Tyr Val Thr Leu Glu Pro Cys Val 
                85                  90                  95      


Met Cys Val Gly Ala Met Leu His Ala Arg Ile Lys Arg Leu Val Phe 
            100                 105                 110         


Gly Ala Ile Asp Gln Lys Thr Gly Ala Val Val Ser Val Phe Lys Ile 
        115                 120                 125             


Leu Asp Glu Lys Asn Leu Asn His Arg Ile Gln Tyr Gln Ser Gly Val 
    130                 135                 140                 


Leu Ala Val Pro Cys Gly Lys Ile Leu Ser Asp Phe Phe Lys Asp Arg 
145                 150                 155                 160 


Arg Gly Val Ile 
                


<210> 427
<211> 180
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-45 deaminase

<400> 427
Met Thr Ala Leu Ile Thr Thr Ser Asn Ile His Asp Lys Met Met Ser 
1               5                   10                  15      


Leu Ala Leu Lys Glu Ala Phe Lys Ala Tyr Lys Leu Gly Glu Ile Pro 
            20                  25                  30          


Val Gly Cys Val Ile Thr Asp Glu Asn Asn Glu Leu Val Ala Thr Gly 
        35                  40                  45              


Phe Asn Arg Thr Ile Ile Asp Asn Asp Pro Ser Ala His Ala Glu Ile 
    50                  55                  60                  


Val Ala Leu Arg Lys Ala Gly Glu Lys Leu Gln Asn Tyr Arg Leu Val 
65                  70                  75                  80  


Asn Leu Asn Leu Tyr Val Thr Leu Glu Pro Cys Thr Met Cys Ser Gly 
                85                  90                  95      


Ala Ile Ile His Ala Arg Ile Lys Arg Val Phe Phe Gly Ala Tyr Asp 
            100                 105                 110         


Leu Lys Thr Gly Ala Cys Gly Ser Val Phe Asn Val Leu Asn Asp Asp 
        115                 120                 125             


Arg His Asn His Lys Val Glu Ile Tyr Gly Gly Ile Leu Lys Glu Glu 
    130                 135                 140                 


Cys Ser Ser Met Leu Ser Asp Phe Phe Lys Ala Arg Arg Lys Met Gln 
145                 150                 155                 160 


Lys Glu Gly Arg Asp Trp Ile Val Glu Ser Gly Lys Asp Arg Thr Trp 
                165                 170                 175     


Leu Asn Lys Asp 
            180 


<210> 428
<211> 153
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-46 deaminase

<400> 428
Met Ile Ser Asp Thr Glu Phe Met Gly Ala Ala Leu Glu Leu Ala Leu 
1               5                   10                  15      


Ala Ala Ser Lys Leu Gly Glu Val Pro Val Gly Ala Val Val Val Lys 
            20                  25                  30          


Gly Gly Arg Ile Ile Gly Thr Gly Ser Asn Ala Pro Ile Ser Arg His 
        35                  40                  45              


Asp Pro Thr Ala His Ala Glu Ile Leu Ala Leu Arg Ala Ala Ala Ser 
    50                  55                  60                  


Ala Leu Glu Asn Tyr Arg Leu Thr Glu Cys Thr Leu Tyr Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Ala Met Cys Ala Gly Ala Ile Gln His Ala Arg Ile Ala 
                85                  90                  95      


Arg Leu Val Phe Gly Ala Ser Asp Ser Lys Thr Gly Ala Cys Gly Ser 
            100                 105                 110         


Val Val Asn Leu Phe Gly Glu Pro Met Leu Asn His His Thr Gln Val 
        115                 120                 125             


Gln Gly Gly Met Leu Ala Arg Asp Cys Gly Glu Leu Leu Ser Asp Phe 
    130                 135                 140                 


Phe Arg Gln Arg Arg Ala Ala Val Gln 
145                 150             


<210> 429
<211> 160
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-47 deaminase

<400> 429
Met Ser Gly Ser Asp Ala Ala Ala Ser Arg Ala Cys Met Glu Gln Ala 
1               5                   10                  15      


Leu Ala Cys Ala Arg Asp Ala Glu Ala Ala Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Val Ile Val Asp Ala Ala Gly Ala Val Val Ala Arg Ala Ala Asn 
        35                  40                  45              


Ala Pro Ile Ala Arg Asn Asp Pro Thr Ala His Ala Glu Leu Leu Val 
    50                  55                  60                  


Leu Arg Ala Ala Gly Arg Val Leu Gly Asn Tyr Arg Leu Pro Gly Cys 
65                  70                  75                  80  


Val Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Val Gly Ala Leu 
                85                  90                  95      


Val His Ala Arg Ile Ala Arg Ile Val Tyr Gly Ala Pro Asp Pro Lys 
            100                 105                 110         


Thr Gly Ala Cys Gly Ser Val Phe Asp Leu Ala Ala Asn Ser Lys Met 
        115                 120                 125             


Asn His Arg Ile Glu Val Ala Gly Gly Val Leu Ala Glu Glu Cys Gly 
    130                 135                 140                 


Ala Leu Leu Lys Gln Phe Phe Ala Ala Arg Arg Gly Arg Asp Ala Pro 
145                 150                 155                 160 


<210> 430
<211> 151
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-48 deaminase

<400> 430
Met Phe Ser Arg Ala Asp Gln Val Phe Met Arg Gln Ala Leu Glu Leu 
1               5                   10                  15      


Ala Lys Gln Ala Lys Gly Glu Gly Glu Val Pro Val Gly Ala Val Leu 
            20                  25                  30          


Thr Leu Asn Asp Leu Pro Ile Ala Thr Ala Tyr Asn Gln Ser Ile Thr 
        35                  40                  45              


His Gln Asp Pro Thr Ala His Ala Glu Ile Ile Val Leu Arg Glu Ala 
    50                  55                  60                  


Ala Lys Ile Leu Lys Asn Tyr Arg Leu Val Asn Thr Val Leu Tyr Val 
65                  70                  75                  80  


Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Ile His Ala Arg 
                85                  90                  95      


Val Lys Arg Leu Ile Tyr Ala Thr Ala Asp Pro Arg Thr Gly Ala Cys 
            100                 105                 110         


Gly Ser Ile Phe Asn Leu Ile Asn His Gln Ala Leu Asn His Gln Ile 
        115                 120                 125             


Gln Tyr Glu Ser Gly Leu Leu Ala Glu Glu Ser Lys Leu Leu Leu Gln 
    130                 135                 140                 


Gln Phe Phe Gln Lys Arg Arg 
145                 150     


<210> 431
<211> 148
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-49 deaminase

<400> 431
Met Asn Asp Glu His Tyr Ile Gln Tyr Ala Leu Asn Leu Ala Gln Lys 
1               5                   10                  15      


Ala Glu Ser Glu Asn Glu Val Pro Val Gly Ala Leu Ile Val Cys Asn 
            20                  25                  30          


Asn Gln Ile Ile Ala Glu Gly Trp Asn Gln Pro Ile Gln Leu His Asp 
        35                  40                  45              


Ser Ser Ala His Ala Glu Met Leu Ala Leu Arg Lys Ala Ser His His 
    50                  55                  60                  


Ile Glu Asn Tyr Arg Leu Lys Asn Thr Thr Leu Tyr Val Thr Leu Glu 
65                  70                  75                  80  


Pro Cys Ala Met Cys Val Gly Ala Met Ile His Ala Arg Ile Gln Lys 
                85                  90                  95      


Leu Val Phe Gly Ala Trp Asp Gln Lys Ala Gly Ala Val His Ser Val 
            100                 105                 110         


Phe Gln Leu Ile Asp Asp Thr Arg His Asn His Ala Ile Glu Trp Arg 
        115                 120                 125             


Gly Gly Val Met Ala Asp Ala Cys Ser Arg Val Leu Lys Asn Phe Phe 
    130                 135                 140                 


Lys Gln Arg Arg 
145             


<210> 432
<211> 153
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-50 deaminase

<400> 432
Met Thr Ser His Asp Asp Thr Tyr Trp Ile Ile His Ala Leu Thr Leu 
1               5                   10                  15      


Ala Lys Lys Ala Glu Gln Gln Gly Glu Val Pro Val Gly Ala Val Ile 
            20                  25                  30          


Val His Asp His Gln Ile Val Gly Glu Gly Trp Asn Gln Pro Ile Gly 
        35                  40                  45              


Ala Gly Asp Pro Thr Ala His Ala Glu Ile Ile Ala Met Arg Asn Ala 
    50                  55                  60                  


Ala Gln His Leu Lys Asn Tyr Arg Leu Ile Asn Cys Thr Leu Tyr Val 
65                  70                  75                  80  


Thr Leu Glu Pro Cys Ala Met Cys Val Gly Ala Met Val His Ala Arg 
                85                  90                  95      


Ile Ser Arg Leu Val Phe Gly Ala Phe Asp Pro Lys Ala Gly Ala Val 
            100                 105                 110         


Thr Ser Val Met Gln Leu Leu Asp Ala Pro His Phe Asn His Ile Val 
        115                 120                 125             


Thr His Gln Gly Gly Ile Cys Ala Glu Lys Cys Ser Glu Ile Leu Lys 
    130                 135                 140                 


Ser Phe Phe Ala Met Lys Arg Lys Thr 
145                 150             


<210> 433
<211> 155
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-51 deaminase

<400> 433
Met Thr Asp Asp Arg Asp Leu Tyr Trp Met Gln Gln Ala Leu Leu Leu 
1               5                   10                  15      


Ala Val Asp Ala Gly Arg Arg Gly Glu Val Pro Val Gly Ala Leu Leu 
            20                  25                  30          


Val Leu Asp Glu Val Ser Ile Gly Val Gly Tyr Asn Arg Pro Ile Gly 
        35                  40                  45              


Asp Arg Asp Pro Thr Ala His Ala Glu Ile Ile Ala Leu Arg Asp Ala 
    50                  55                  60                  


Ala Lys Lys Ile Asn Asn Tyr Arg Leu Leu Asp Thr Thr Leu Tyr Val 
65                  70                  75                  80  


Thr Leu Glu Pro Cys Met Met Cys Ala Ala Ala Met Val His Ala Arg 
                85                  90                  95      


Ile Lys Arg Leu Val Tyr Gly Ala Pro Asp Pro Lys Ala Gly Ala Ile 
            100                 105                 110         


Val Ser Gln Ala Gln Ile Leu Asp Ala Pro Phe Phe Asn His Arg Val 
        115                 120                 125             


Ala Tyr Tyr Gly Asn Leu Leu Ala Met Pro Cys Gly Glu Leu Leu Arg 
    130                 135                 140                 


Thr Phe Phe Lys Ala Lys Arg Leu Ser Ser Thr 
145                 150                 155 


<210> 434
<211> 153
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-52 deaminase

<400> 434
Met Arg Leu Ala Met Asp Met Ala Lys Glu Ala Tyr Ser Ile Gly Glu 
1               5                   10                  15      


Val Pro Val Gly Cys Val Ile Ala Asp Ala Asp Gly Asn Val Ile Ser 
            20                  25                  30          


Thr Gly Tyr Asn Arg Ser Ile Thr Asp Asn Asp Pro Ser Ala His Ala 
        35                  40                  45              


Glu Met Val Ala Ile Arg Asn Ala Gly Ser Leu Leu Lys Asn Tyr Arg 
    50                  55                  60                  


Met Pro Gly Leu Ser Leu Tyr Val Thr Leu Glu Pro Cys Cys Met Cys 
65                  70                  75                  80  


Ser Gly Ala Ile Ile His Ala Arg Ile Ala Arg Val Tyr Tyr Gly Ala 
                85                  90                  95      


Ser Asp Leu Lys Thr Gly Ala Cys Gly Ser Val Phe Asn Val Met Ser 
            100                 105                 110         


Asp Pro Arg His Asn His Ser Val Glu Cys Ile Gly Gly Ile Leu Ala 
        115                 120                 125             


Asp Glu Cys Ser Lys Met Leu Ser Asp Phe Phe Lys Glu Arg Arg Gln 
    130                 135                 140                 


Gln Lys Lys Ala Glu Ala Thr Lys Leu 
145                 150             


<210> 435
<211> 174
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-53 deaminase

<400> 435
Met Thr Val Asn His Glu Tyr Phe Met Arg Leu Ala Ile Glu Gln Ala 
1               5                   10                  15      


Leu Leu Ala Tyr Gln Ala Gly Glu Ile Pro Val Gly Ala Val Ile Val 
            20                  25                  30          


Asp Thr Asp Gly Asn Val Ile Gly Arg Gly Phe Asn Arg Thr Ile Leu 
        35                  40                  45              


Asp Asn Asp Pro Ser Ala His Ala Glu Ile Val Ala Leu Arg Asp Ala 
    50                  55                  60                  


Gly Glu Arg Val Lys Asn Tyr Arg Leu Ile Asn Leu Asp Met Tyr Val 
65                  70                  75                  80  


Thr Leu Glu Pro Cys Thr Met Cys Ser Gly Ala Leu Ile His Ser Arg 
                85                  90                  95      


Leu Asn Asn Leu Tyr Phe Gly Ala Tyr Asp Leu Lys Thr Gly Ala Cys 
            100                 105                 110         


Gly Ser Arg Phe Asn Ile Leu Gly Ser Ser Glu Tyr Asn His Ile Val 
        115                 120                 125             


Asn Val Thr Gly Gly Ile Leu Lys Glu Glu Cys Gln Asn Ile Ile Ser 
    130                 135                 140                 


Asp Phe Phe Lys Ala Arg Arg Lys Met Gln Lys Asp Gly Arg Asp Trp 
145                 150                 155                 160 


Ile Val Glu Ser Gly Lys Val Arg Ser Trp Gln Lys Asn Val 
                165                 170                 


<210> 436
<211> 172
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-54 deaminase

<400> 436
Met Thr Ser Pro Thr Ala Gly Leu Gly Glu Thr Asp Glu Tyr Trp Met 
1               5                   10                  15      


Asn Arg Ala Leu Ala Leu Ala Ala Glu Ala Gly Glu Ser Gly Glu Val 
            20                  25                  30          


Pro Val Gly Ala Val Val Val Leu Asp Gly Arg Glu Val Gly Ala Gly 
        35                  40                  45              


Tyr Asn Gly Pro Ile Gly Ser Cys Asp Pro Thr Ala His Ala Glu Ile 
    50                  55                  60                  


Arg Ala Leu Arg Asp Ala Gly Ala Arg Ala Gly Asn Tyr Arg Leu Pro 
65                  70                  75                  80  


Gly Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Thr Met Cys Val Gly 
                85                  90                  95      


Ala Ile Val His Ala Arg Ile Asn Arg Leu Val Tyr Gly Thr Thr Glu 
            100                 105                 110         


Pro Lys Ala Gly Ala Val Glu Ser Ile Arg Gln Thr Leu Arg Asp Asp 
        115                 120                 125             


His Phe Asn Trp Gln Val Glu Ala Thr Gly Gly Val Leu Ala Ser Gln 
    130                 135                 140                 


Cys Arg Glu Ile Ile Gln Arg Phe Phe Ala Gly Arg Arg Glu Tyr His 
145                 150                 155                 160 


Arg Leu Arg Lys Gln Thr Leu Ser Val Lys Glu Gln 
                165                 170         


<210> 437
<211> 158
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-55 deaminase

<400> 437
Met Pro Asp Leu Asp Asp Gly Tyr Trp Met Gln Gln Ala Leu Lys Leu 
1               5                   10                  15      


Ala Ala Gln Ala Lys Asp Gln Gly Glu Val Pro Val Gly Ala Leu Val 
            20                  25                  30          


Val Gly Glu Gln Lys Leu Leu Gly Gln Gly Tyr Asn Gln Val Ile Ser 
        35                  40                  45              


Leu Gln Asp Pro Ser Ala His Ala Glu Val Met Ala Leu Arg Gly Ala 
    50                  55                  60                  


Gly Ile Thr Gln Gln Asn Tyr Arg Leu Val Asn Thr Thr Leu Tyr Val 
65                  70                  75                  80  


Thr Leu Glu Pro Cys Met Met Cys Ala Gly Val Leu Val His Ala Arg 
                85                  90                  95      


Ile Lys Arg Leu Val Phe Gly Ala Tyr Asp Pro Lys Ala Gly Ala Ile 
            100                 105                 110         


Ser Ser Cys Ala Gln Leu Leu Thr Leu Pro His Leu Asn His Gln Ile 
        115                 120                 125             


Leu Val Gln Gly Gly Ile Leu Ala Lys Glu Cys Gly Gln Gln Leu Thr 
    130                 135                 140                 


Asp Phe Phe Lys Ala Lys Arg Gln Ala Lys His Asp Lys Gly 
145                 150                 155             


<210> 438
<211> 157
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-56 deaminase

<400> 438
Met Thr Ala Ala Ala Glu Phe Asp Glu His Asp Leu Arg Trp Met Arg 
1               5                   10                  15      


Gln Ala Leu Glu Leu Ala Arg Arg Ala Glu Arg Asp His Ala Glu Val 
            20                  25                  30          


Pro Val Gly Ala Val Leu Val Ile Asp Asn Glu Val Ile Gly Ser Gly 
        35                  40                  45              


Met Asn Arg Asn Ile Ile Asp His Asp Ala Ser Ala His Ala Glu Ile 
    50                  55                  60                  


Val Cys Leu Arg Asp Ala Gly Gln Arg Val Ala Asn His Arg Phe Pro 
65                  70                  75                  80  


Gly Ser Thr Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly 
                85                  90                  95      


Ala Ile Val His Ala Arg Val Gly Arg Val Val Tyr Ala Ala Ser Asp 
            100                 105                 110         


Pro Lys Thr Gly Ala Ala Gly Ser Val Phe Asp Ile Leu Thr Ser Glu 
        115                 120                 125             


Arg His Asn His Arg Val Ala Val Arg Gly Gly Leu Leu Ala Glu Glu 
    130                 135                 140                 


Ser Ala Arg Leu Leu Gln Leu Phe Phe Arg Ser Arg Arg 
145                 150                 155         


<210> 439
<211> 176
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-57 deaminase

<400> 439
Met Ser Glu Ser Ser Ala Val Tyr Ser Asp Glu Tyr Trp Leu Arg Tyr 
1               5                   10                  15      


Ala Leu Gln Leu Ala Glu Arg Ala Tyr Gln Gln Gly Glu Val Pro Val 
            20                  25                  30          


Gly Ala Val Leu Val Arg Asp Asn Gln Ile Leu Gly Glu Gly Trp Asn 
        35                  40                  45              


Gln Ser Ile Ser Gln His Asp Ala Thr Ala His Ala Glu Ile Met Ala 
    50                  55                  60                  


Leu Arg Ala Ala Gly Leu Val Ala Gly Asn Tyr Arg Leu Pro Asn Thr 
65                  70                  75                  80  


Ser Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Leu 
                85                  90                  95      


Leu His Ala Arg Val Glu Arg Val Val Phe Gly Ala Tyr Asp Pro Lys 
            100                 105                 110         


Thr Gly Ala Val Gly Ser Met Phe Asp Val Leu Leu Asp Ala Arg His 
        115                 120                 125             


Asn His Gln Val Gln Leu Thr Gly Gly Val Leu Gln Ala Asp Cys Val 
    130                 135                 140                 


Ala Gln Leu Gln Ala Phe Phe Lys Glu Arg Arg Leu Ala Gln Lys Leu 
145                 150                 155                 160 


Ala Lys Phe Asn Ser Val Thr Ile Ser Ser Pro Thr Ser Thr Glu Asn 
                165                 170                 175     


<210> 440
<211> 158
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-58 deaminase

<400> 440
Met Ser Leu Ala Asp Arg His Glu Met Phe Met Gln Met Ala Leu Gln 
1               5                   10                  15      


Gln Ala Gln Leu Ala Tyr Asp Cys Ala Glu Val Pro Val Gly Ala Val 
            20                  25                  30          


Val Val Asn Ser Ala Gly Glu Val Ile Gly Lys Gly Tyr Asn Arg Thr 
        35                  40                  45              


Ile Ile Asp Ser Asp Pro Thr Ala His Ala Glu Ile Val Ala Leu Arg 
    50                  55                  60                  


Gln Ala Ala Ala His Glu Gln Asn Tyr Arg Leu Pro Asp Leu Thr Val 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ile Gly Ala Leu Thr His 
                85                  90                  95      


Ala Arg Ile Ala His Val Val Tyr Gly Ala Ser Asp Pro Lys Thr Gly 
            100                 105                 110         


Ala Cys Gly Ser Val Leu Cys Ile Gln Ala Asn Lys Lys Ile Asn His 
        115                 120                 125             


His Thr Arg Ile Thr Ser Gly Val Leu Glu Gln Ala Cys Ala Glu Lys 
    130                 135                 140                 


Leu Arg His Phe Phe Arg Glu Arg Arg Lys Gly Ser Lys Leu 
145                 150                 155             


<210> 441
<211> 154
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-59 deaminase

<400> 441
Met Ala Asp Asp Asp Phe Met Ala Leu Ala Ile Glu Gln Ala Leu Leu 
1               5                   10                  15      


Ala Gln Ala Gln Gly Glu Val Pro Val Gly Ala Val Leu Val Asp Gly 
            20                  25                  30          


Ser Gly His Val Ile Gly Arg Gly Ala Asn Ala Val Ile Gly Leu Ser 
        35                  40                  45              


Asp Pro Thr Ala His Ala Glu Ile Val Ala Leu Arg Ser Ala Ala Lys 
    50                  55                  60                  


Leu Leu Gly Asn Tyr Arg Leu Pro Gln Thr Thr Leu Phe Val Thr Leu 
65                  70                  75                  80  


Glu Pro Cys Ala Met Cys Leu Gly Ala Leu Phe His Ala Arg Val Ser 
                85                  90                  95      


Arg Ile Val Phe Gly Ala Pro Asp Pro Lys Thr Gly Ala Cys Gly Ser 
            100                 105                 110         


Arg Leu Asp Leu Thr Ala Pro Asp Leu Ile Asn Phe His Ala Ser Val 
        115                 120                 125             


Arg Gly Gly Val Met Gln Glu Ala Cys Gly His Leu Leu Thr Asp Phe 
    130                 135                 140                 


Phe Lys Arg Lys Arg Ser Glu Lys Leu Ser 
145                 150                 


<210> 442
<211> 204
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-60 deaminase

<400> 442
Met Arg Val Ala Leu Gly Ala Ala Arg Glu Ala Gln Ala Ala Gly Glu 
1               5                   10                  15      


Val Pro Val Gly Ala Val Ile Val Arg Asp Gly Val Leu Ile Ala Thr 
            20                  25                  30          


Gly Arg Asn Ala Pro Arg Gln Gln His Asp Pro Thr Ala His Ala Glu 
        35                  40                  45              


Ile Ala Ala Leu Arg Ala Ala Ala Arg Arg Leu Gly Asn Tyr Arg Leu 
    50                  55                  60                  


Asp Gly Cys Thr Leu Tyr Val Thr Leu Glu Pro Cys Leu Met Cys Val 
65                  70                  75                  80  


Gly Ala Met Leu His Ala Arg Leu Glu Arg Val Val Phe Gly Ala Pro 
                85                  90                  95      


Asp Pro Lys Thr Gly Ala Ala Gly Ser Val Phe Asn Ala Leu Ala Glu 
            100                 105                 110         


Pro Lys Leu Asn His Gln Thr Thr Val Glu Gly Gly Val Leu Ala Thr 
        115                 120                 125             


Glu Cys Ala Ala Leu Leu Gln Val Phe Phe Gly Gln Arg Arg Leu Leu 
    130                 135                 140                 


Gln Arg Ile Ser Ala Gln Pro Leu Arg Asp Asp Ala Leu Arg Thr Ser 
145                 150                 155                 160 


Glu Ala Arg Met Ala Ala Phe Ala Ser Pro Leu Ser Arg Trp Val Cys 
                165                 170                 175     


Asp Leu Pro Ala Leu Gln Gly Leu Arg Leu His Tyr Leu Asp Met Gly 
            180                 185                 190         


Pro Ser Thr Glu Gly Arg Ala Trp Leu Leu Leu His 
        195                 200                 


<210> 443
<211> 168
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-61 deaminase

<400> 443
Met Asn Thr Glu Gln Ser Lys Pro Leu Leu Gly Asp Ala His Phe Met 
1               5                   10                  15      


Gln Gln Ala Leu Gln Leu Ala Asp Ala Ala Phe Ala Leu Gln Glu Val 
            20                  25                  30          


Pro Val Gly Ala Val Val Val Gln Asp Gly Ile Ile Ile Gly Arg Gly 
        35                  40                  45              


Phe Asn Ala Pro Ile Thr Gln His Asp Pro Cys Ala His Ala Glu Ile 
    50                  55                  60                  


Leu Ala Leu Arg Asp Ala Ala Lys Asn Ile Gly Asn Tyr Arg Leu Val 
65                  70                  75                  80  


Gly Cys Glu Leu Phe Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly 
                85                  90                  95      


Ala Met Met His Ala Arg Ile Ala Arg Val Val Tyr Gly Ala Tyr Asp 
            100                 105                 110         


Leu Lys Thr Gly Ala Cys Gly Ser Val Gln Asn Val Phe Asn Glu Pro 
        115                 120                 125             


Arg Trp Asn His His Thr Ile Cys Thr Gly Gly Val Leu Ala Glu Gln 
    130                 135                 140                 


Cys Gly Thr Lys Leu Ser Gln Phe Phe Ala Leu Arg Arg Ala Gln Lys 
145                 150                 155                 160 


Lys Ser Leu Thr Ala Thr Trp Thr 
                165             


<210> 444
<211> 283
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG121 deaminase

<220>
<223> Description: MG121-1 deaminase

<400> 444
Met Lys Val Thr Leu Gly Gly Gln Thr Ile Asp Val Glu Glu Val Leu 
1               5                   10                  15      


Lys Arg Ile Ser Ser Thr Thr Val Ala Ala Ala Leu Ile Lys Asp Gly 
            20                  25                  30          


Glu Val Val Ala Val Glu Glu Thr Gly Gly Glu His Ala Glu Lys Lys 
        35                  40                  45              


Leu Val Arg Lys His Asp Val Glu Gly Ala Val Ile Phe Val Thr Ala 
    50                  55                  60                  


Arg Pro Cys Arg Tyr Cys Ala Glu Ala Met Ala Glu Ala Gly Val Ala 
65                  70                  75                  80  


Gly Val Val Tyr Leu Gly Lys Gly Arg Gly Tyr Gly Pro Tyr Leu Leu 
                85                  90                  95      


Glu Arg Leu Gly Val Pro Cys Val Glu Val His Pro Ser Glu Pro Pro 
            100                 105                 110         


Ala Trp Arg Pro Val Pro Arg Leu Asp Val Leu Ile Thr Phe Gly Gly 
        115                 120                 125             


Asn Pro Tyr Leu Thr Glu Glu Asp Val Ala Ala Arg Thr Tyr Ala Leu 
    130                 135                 140                 


Leu Thr Gly Ile Gly Val Glu Ala Glu Val Val Pro Ala Pro Asn Asn 
145                 150                 155                 160 


Met Ala Gly Arg Val Glu Val Met Val Lys Arg Gly Asp Pro Asp Arg 
                165                 170                 175     


Ala His Gly Arg Leu Lys Glu Thr Phe Gly Pro Val Phe Lys Val Arg 
            180                 185                 190         


Arg Phe Gln Leu Ser Gly Asp Tyr Glu Asp Arg Glu Ser Leu Ala Glu 
        195                 200                 205             


Asp Val Leu Glu Val Val Glu Pro Leu Ile Glu Asp Pro Phe Ala Val 
    210                 215                 220                 


Arg Ala Asn Val Ala Ser Pro Gly Pro Thr Phe Ser Gly Ser Lys Glu 
225                 230                 235                 240 


Ala Glu Val Phe Ile Gly Asp Val Leu Thr Ser Ala Gly His Glu Val 
                245                 250                 255     


Asp Leu Glu Asp Pro Arg Thr Leu Val His Val Asp Val Leu Gly Arg 
            260                 265                 270         


Arg Val Ser Val Gly Val Glu Arg Arg Asp Gly 
        275                 280             


<210> 445
<211> 279
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG121 deaminase

<220>
<223> Description: MG121-2 deaminase

<400> 445
Val Lys Ile Thr Leu Ala Gly Glu Thr Ile Asp Val Glu Lys Val Leu 
1               5                   10                  15      


Arg Glu Ile Pro Thr Arg Thr Val Thr Ala Ala Leu Ile Lys Asp Gly 
            20                  25                  30          


Glu Val Lys Ala Tyr Glu Glu Ala Asp Gly Glu His Ala Glu Lys Lys 
        35                  40                  45              


Leu Ile Arg Asn His Asp Val Ser Asp Gly Val Val Phe Val Thr Ile 
    50                  55                  60                  


Arg Pro Cys Leu Tyr Cys Ala Arg Glu Leu Ala Lys Ala Gly Val Ala 
65                  70                  75                  80  


Gly Val Val Tyr Ile Gly Arg Gly Arg Gly Leu Gly Pro Tyr Tyr Leu 
                85                  90                  95      


Ala Arg Lys Gly Ile Glu Val Val Glu Val Asn Pro Ser Arg Pro Leu 
            100                 105                 110         


His Tyr Asp Pro Val Glu Arg Leu Asp Val Leu Val Thr Phe Gly Gly 
        115                 120                 125             


Asn Pro Tyr Leu Thr Glu Glu Asp Val Ala Ala Arg Val Tyr Cys Leu 
    130                 135                 140                 


Leu Thr Gly Lys Gly Ile Lys Ala Glu Ile Ala Pro Ala Pro Glu Asn 
145                 150                 155                 160 


Leu Ala Gly Arg Val Glu Val Met Ile Thr Asp Gly Asp Ser Glu Arg 
                165                 170                 175     


Ala Ala Arg Val Ile Glu Arg Glu Leu Pro Thr Phe Asn Val Arg His 
            180                 185                 190         


Phe Leu Leu Ser Asp Arg Phe Asp Asp Arg Glu Glu Leu Ser Lys Arg 
        195                 200                 205             


Val Leu Glu Glu Ile Glu Pro Arg Val Glu Asp Pro Phe Ala Val Arg 
    210                 215                 220                 


Ala Arg Ile Ala Arg Ala Gly Leu Phe Ser Ser Ser Arg Asp Ala Glu 
225                 230                 235                 240 


Val Phe Leu Gly Asp Val Leu Thr Ser Ala Gly His Glu Val Asp Leu 
                245                 250                 255     


Asp Glu Pro Arg Thr Val Val His Val Asp Val Leu Gly Asp Arg Val 
            260                 265                 270         


Ser Val Gly Val Glu Lys Arg 
        275                 


<210> 446
<211> 283
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG121 deaminase

<220>
<223> Description: MG121-3 deaminase

<400> 446
Met Lys Val Thr Leu Gly Gly Gln Thr Ile Asp Val Glu Glu Val Leu 
1               5                   10                  15      


Lys Arg Ile Ser Ser Thr Thr Val Ala Ala Ala Leu Ile Lys Asp Gly 
            20                  25                  30          


Glu Val Val Ala Val Glu Glu Thr Gly Gly Glu His Ala Glu Lys Lys 
        35                  40                  45              


Leu Val Arg Lys His Asp Val Glu Gly Ala Val Ile Phe Val Thr Ala 
    50                  55                  60                  


Arg Pro Cys Arg Tyr Cys Ala Glu Ala Met Ala Glu Ala Gly Val Ala 
65                  70                  75                  80  


Gly Val Val Tyr Leu Gly Lys Gly Arg Gly Tyr Gly Pro Tyr Leu Leu 
                85                  90                  95      


Glu Arg Leu Gly Val Pro Cys Val Glu Val His Pro Ser Glu Pro Pro 
            100                 105                 110         


Ala Trp Arg Pro Val Pro Arg Leu Asp Val Leu Ile Thr Phe Gly Gly 
        115                 120                 125             


Asn Pro Tyr Leu Thr Glu Glu Asp Val Ala Ala Arg Thr Tyr Ala Leu 
    130                 135                 140                 


Leu Thr Gly Ile Gly Ile Glu Ala Glu Val Val Pro Ala Pro Asn Asn 
145                 150                 155                 160 


Met Ala Gly Arg Val Glu Val Met Val Lys Arg Gly Asp Pro Asp Arg 
                165                 170                 175     


Ala His Gly Arg Leu Lys Glu Thr Phe Gly Ser Val Phe Lys Val Arg 
            180                 185                 190         


Arg Phe Gln Leu Ser Gly Asp Tyr Glu Asp Arg Glu Ser Leu Ala Glu 
        195                 200                 205             


Asp Val Leu Glu Val Val Glu Pro Leu Ile Glu Asp Pro Phe Ala Val 
    210                 215                 220                 


Arg Ala Asn Val Ala Ser Pro Gly Pro Thr Phe Ser Gly Ser Lys Glu 
225                 230                 235                 240 


Ala Glu Val Phe Ile Gly Asp Val Leu Thr Ser Ala Gly His Glu Val 
                245                 250                 255     


Asp Leu Glu Asp Pro Arg Thr Leu Val His Val Asp Val Leu Gly Arg 
            260                 265                 270         


Arg Val Ser Val Gly Val Glu Arg Arg Asp Gly 
        275                 280             


<210> 447
<211> 280
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      uncultivated organism

<220>
<223> Category: MG121 deaminase

<220>
<223> Description: MG121-4 deaminase

<400> 447
Met Arg Ser Ala Glu Tyr Leu Ala Tyr Leu Arg Arg Arg Tyr Gly Leu 
1               5                   10                  15      


Arg Pro Asn Arg Arg Leu Gly Gln His Phe Met Val Asp Asp Arg Leu 
            20                  25                  30          


Leu Arg Phe Met Val Glu Ala Ala Gly Val Gly Pro Glu Asp Thr Val 
        35                  40                  45              


Leu Glu Ile Gly Pro Gly Pro Gly Leu Leu Thr Arg Tyr Leu Ile Glu 
    50                  55                  60                  


Arg Ala Gly Arg Val Val Ala Val Glu Leu Asp Glu Arg Leu Val Glu 
65                  70                  75                  80  


Val Leu Glu Arg Glu Leu Gly Asp Ala Glu Asn Leu Glu Ile Val His 
                85                  90                  95      


Ala Asp Phe Leu Glu Tyr Gly Val Pro Glu Glu Val Asn Lys Val Val 
            100                 105                 110         


Ser Asn Leu Pro Tyr Gln Ile Ser Ser Glu Ala Thr Phe Glu Ile Leu 
        115                 120                 125             


Arg Ser Asp Val Glu Arg Ala Val Leu Thr Tyr Gln Arg Glu Phe Ala 
    130                 135                 140                 


Glu Arg Met Thr Ala Glu Pro Gly Thr Arg Gly Tyr Gly Arg Leu Thr 
145                 150                 155                 160 


Val Met Val Asn Leu Leu Ala Arg Val Glu Val Leu Arg Ala Val Pro 
                165                 170                 175     


Arg Arg Ala Phe Val Pro Pro Pro Arg Val Ser Ser Ala Ile Val Arg 
            180                 185                 190         


Leu Glu Pro Arg Pro Glu Ser Glu Leu Pro Asp Val Asp Pro Glu Thr 
        195                 200                 205             


Leu Glu Ala Val Cys Arg Gly Leu Phe Gln His Arg Arg Lys Thr Val 
    210                 215                 220                 


Arg Asn Ala Leu Arg Leu Ser Ala His Glu Trp Gly Gly Asp Glu Glu 
225                 230                 235                 240 


Val Val Glu Glu Val Leu Arg Arg Leu Pro Glu Glu Leu Leu Asp Lys 
                245                 250                 255     


Arg Pro Leu His Leu Thr Pro Glu Glu Val Val Arg Val Ala Arg Cys 
            260                 265                 270         


Val Glu Arg Ala Arg Gly Ser Ser 
        275                 280 


<210> 448
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V1

<400> 448
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 449
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V2

<400> 449
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 450
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V3

<400> 450
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 451
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V4

<400> 451
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 452
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V5

<400> 452
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 453
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V6

<400> 453
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 454
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V7

<400> 454
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Cys Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 455
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V8

<400> 455
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 456
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V9

<400> 456
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 457
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V10

<400> 457
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 458
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V11

<400> 458
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Arg 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 459
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V12

<400> 459
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Arg Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 460
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V13

<400> 460
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Pro Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 461
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V14

<400> 461
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 462
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V15

<400> 462
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 463
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V16

<400> 463
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 464
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V17

<400> 464
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Ser Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 465
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V18

<400> 465
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 466
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V19

<400> 466
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 467
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V20

<400> 467
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 468
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V21

<400> 468
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 469
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V22

<400> 469
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Arg Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 470
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V23

<400> 470
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 471
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V24

<400> 471
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg Tyr Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 472
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V25

<400> 472
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Tyr Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 473
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V26

<400> 473
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Arg Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 474
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V27

<400> 474
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Arg 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Arg Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 475
<211> 167
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: MG68 putative adenosine deaminase (TadA-like)

<220>
<223> Description: MG68-4_V28

<400> 475
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Arg Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Ser Thr Phe Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Val Glu Asn Pro Lys Arg 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Asn Arg 
    130                 135                 140                 


Leu Leu Cys Asp Phe Phe Arg Trp Arg Pro Ala Gln Lys Asn Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn 
                165         


<210> 476
<211> 962
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: MG68-4_V1-nMG34-1 (D10A)

<400> 476
Met Thr Asp Ser Gln Asn Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala 
1               5                   10                  15      


Leu Lys Leu Ala Gln Lys Ala Trp Asp Glu Gly Glu Val Pro Val Gly 
            20                  25                  30          


Ala Ile Leu Val Arg Lys Gly Glu Val Ile Gly His Gly Trp Asn Arg 
        35                  40                  45              


Ser Ile Gly Ser His Asp Pro Ser Ala His Ala Glu Ile Met Ala Ile 
    50                  55                  60                  


Arg Glu Ala Gly Val Arg Ile Gly Asn Tyr Arg Met Leu Asp Thr Val 
65                  70                  75                  80  


Leu Tyr Val Thr Leu Glu Pro Cys Ala Met Cys Ala Gly Ala Leu Val 
                85                  90                  95      


His Gly Arg Ile Ser Arg Leu Val Phe Gly Ala Glu Asn Pro Lys Thr 
            100                 105                 110         


Gly Ala Cys Gly Ser Val Met Asp Ile Ala Arg His Pro Ala Leu Asn 
        115                 120                 125             


His Gln Leu Gln Val Asp Gly Gly Val Met Ala Ala Pro Cys Ala Arg 
    130                 135                 140                 


Leu Leu Ser Asp Phe Phe Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala 
145                 150                 155                 160 


Arg Asp Asn Ser Ser Gly Asn Gly Thr Ser Gly Gly Ser Ser Gly Gly 
                165                 170                 175     


Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
            180                 185                 190         


Ser Ser Gly Gly Ser Ser Gly Gly Ser Met Glu Arg Glu Leu Val Leu 
        195                 200                 205             


Gly Ile Ala Tyr Gly Gly Lys Tyr Thr Gly Leu Ala Val Val Asp Arg 
    210                 215                 220                 


Arg His Asn Gln Val Leu Tyr Ala Asn Arg Leu Lys Met Arg Asp Asp 
225                 230                 235                 240 


Val Ala Gly Ile Leu Lys Asp Arg Arg Lys Gln Arg Gly Ile Arg Arg 
                245                 250                 255     


Thr Ala Gln Thr Lys Lys Lys Arg Leu Arg Glu Leu Lys Asn Tyr Leu 
            260                 265                 270         


Lys Ser Ile Gly Tyr Asn Glu Ser Thr Ala Thr Phe Glu Thr Val Tyr 
        275                 280                 285             


Ser Leu Ala His Lys Arg Gly Tyr Asp Tyr Ala Asp Met Pro Glu Glu 
    290                 295                 300                 


Lys Thr Ser Glu Glu Ile Glu Ala Met Asp Val Glu Glu Arg Lys Gln 
305                 310                 315                 320 


Trp Glu Lys Glu Lys Gln Glu Trp Glu Glu Thr Lys Arg Asn Ser Arg 
                325                 330                 335     


His Arg Lys Glu Val Val Lys Asp Val His Lys Ala Met Ile Glu Gly 
            340                 345                 350         


Arg Ala Thr Glu Glu Gln Ile Lys Arg Val Glu Arg Ile Phe Asn Lys 
        355                 360                 365             


Gln Tyr Arg Pro Lys Arg Phe Asn Asn Arg Ile Leu Thr Lys Cys Lys 
    370                 375                 380                 


Val Glu Asp Cys Gly Val Asn Thr Pro Leu Arg Lys Asn Val Arg Asp 
385                 390                 395                 400 


Leu Leu Ile Glu Asn Ile Val Arg Phe Phe Pro Ile Glu Gln Ser Glu 
                405                 410                 415     


Lys Asp Asn Leu Lys Asp Ala Val Leu Asp Lys Asn Arg Arg Glu Glu 
            420                 425                 430         


Val Lys Ser Phe Phe Arg Lys His Lys Thr Asp Glu His Ile Arg Lys 
        435                 440                 445             


Gln Val Tyr Asp Ile Ala Asp Asn Lys Leu Ser Gly Arg Thr Val Phe 
    450                 455                 460                 


Cys Lys Glu His Ile Leu Glu His Thr Glu His Ser Lys Glu Glu Arg 
465                 470                 475                 480 


Lys Val Phe Arg Leu Ala Pro Ser Leu Lys Thr Lys Ile Glu Asn Val 
                485                 490                 495     


Leu Ala Val Ile Lys Asp Glu Ile Leu Pro Lys Phe Thr Val Asn Lys 
            500                 505                 510         


Val Val Met Glu Ser Asn Asn Phe Asp Ile Ala Ala Lys Thr Gln Gly 
        515                 520                 525             


Lys Lys Arg Leu Ala Lys Glu Glu Tyr Gly Lys Gly Pro Arg Glu Gly 
    530                 535                 540                 


Lys Glu Thr Arg Lys Glu Ala Leu Leu Arg Glu Thr Asp Gly Arg Cys 
545                 550                 555                 560 


Ile Tyr Cys Gly Lys Ser Ile Asp Ile Ser Asn Ala His Asp Asp His 
                565                 570                 575     


Ile Phe Pro Arg Lys Ala Gly Gly Leu Asn Ile Phe Ala Asn Leu Val 
            580                 585                 590         


Ala Cys Cys Ala Val Cys Asn Glu Asn Lys Lys Gly Arg Thr Pro Leu 
        595                 600                 605             


Glu Ser Gly Ile Ser Pro Lys Pro Glu Ile Ile Ala Phe Met Lys Asn 
    610                 615                 620                 


Asp Leu Lys Lys Lys Ile Leu Glu Asp Ala Arg Asn Ile Asn Thr Val 
625                 630                 635                 640 


Asp Phe Asn Lys Tyr Met Ser His Ala Ser Ile Gly Trp Arg Tyr Met 
                645                 650                 655     


Arg Asp Arg Leu Arg Glu Ser Ala Gly Asn Lys Lys Leu Pro Ile Glu 
            660                 665                 670         


Arg Gln Ser Gly Ile Tyr Thr Ala Tyr Phe Arg Arg Trp Trp Gly Phe 
        675                 680                 685             


Lys Lys Glu Arg Gly Asn Thr Leu His His Ala Leu Asp Ala Val Ile 
    690                 695                 700                 


Leu Ala Ser Arg Lys Gly Tyr Ser Asp Asp Gly Leu Val Asp Met Thr 
705                 710                 715                 720 


Leu Lys Pro Lys Tyr Asn Lys Gly Gly Glu Phe Asp Pro Glu Lys His 
                725                 730                 735     


Leu Pro Glu Pro Ile Glu Phe Lys Met Asp Lys Gly Ser Arg Gly Ser 
            740                 745                 750         


Ala Leu His Asp Arg Asn Pro Leu Ser Tyr Lys Lys Gly Ile Ile Thr 
        755                 760                 765             


Arg Arg Phe Met Val Thr Glu Ile Glu Cys Gly Lys Glu Asp Asp Val 
    770                 775                 780                 


Ile Ser Glu Thr Tyr Arg Glu Lys Leu Lys Glu Ala Phe Lys Arg Phe 
785                 790                 795                 800 


Asp Thr Lys Lys Gly Lys Cys Leu Thr Asp Lys Glu Ala Lys Glu Ala 
                805                 810                 815     


Gly Phe Cys Ile Lys Lys Asn Glu Leu Val Met Ser Leu Lys Cys Ser 
            820                 825                 830         


Ile Lys Gly Thr Gly Pro Gly Gln Met Ile Arg Ile Asn Asn Asn Val 
        835                 840                 845             


Phe Lys Thr Asn Val His Asn Val Gly Val Asp Val Tyr Leu Asp Glu 
    850                 855                 860                 


Lys Gly Lys Lys Lys Ala Tyr Glu Arg Lys Asn Pro Arg Leu Ser Lys 
865                 870                 875                 880 


His Phe Ile Glu Pro Pro Pro Gln Pro Asn Gly Arg Val Ser Phe Thr 
                885                 890                 895     


Leu Lys Arg Arg Asp Met Val Thr Val Glu Gly Glu Asp Ala Ile Tyr 
            900                 905                 910         


Arg Ile Lys Lys Leu Gly Thr Ser Pro Thr Ile Glu Ala Val Val Gly 
        915                 920                 925             


Ser Asp Gly Lys Thr Arg Thr Val Ser Ala Thr Lys Leu Thr Lys Ala 
    930                 935                 940                 


Asn Ser Ala Glu Leu Glu Gly Ser Gly Gly Ser Pro Lys Lys Lys Arg 
945                 950                 955                 960 


Lys Val 
        


<210> 477
<211> 1589
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: MG68-4_V1-nSpCas9 (D10A)

<400> 477
Met Gly Ser Ser His His His His His His Met Thr Asp Ser Gln Asn 
1               5                   10                  15      


Asp Thr Asp Glu Tyr Trp Met Gly Glu Ala Leu Lys Leu Ala Gln Lys 
            20                  25                  30          


Ala Trp Asp Glu Gly Glu Val Pro Val Gly Ala Ile Leu Val Arg Lys 
        35                  40                  45              


Gly Glu Val Ile Gly His Gly Trp Asn Arg Ser Ile Gly Ser His Asp 
    50                  55                  60                  


Pro Ser Ala His Ala Glu Ile Met Ala Ile Arg Glu Ala Gly Val Arg 
65                  70                  75                  80  


Ile Gly Asn Tyr Arg Met Leu Asp Thr Val Leu Tyr Val Thr Leu Glu 
                85                  90                  95      


Pro Cys Ala Met Cys Ala Gly Ala Leu Val His Gly Arg Ile Ser Arg 
            100                 105                 110         


Leu Val Phe Gly Ala Glu Asn Pro Lys Thr Gly Ala Cys Gly Ser Val 
        115                 120                 125             


Met Asp Ile Ala Arg His Pro Ala Leu Asn His Gln Leu Gln Val Asp 
    130                 135                 140                 


Gly Gly Val Met Ala Ala Pro Cys Ala Arg Leu Leu Ser Asp Phe Phe 
145                 150                 155                 160 


Arg Trp Arg Arg Ala Gln Lys Lys Ala Ala Arg Asp Asn Ser Ser Gly 
                165                 170                 175     


Asn Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly 
            180                 185                 190         


Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly 
        195                 200                 205             


Ser Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser 
    210                 215                 220                 


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
225                 230                 235                 240 


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
                245                 250                 255     


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
            260                 265                 270         


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
        275                 280                 285             


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
    290                 295                 300                 


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
305                 310                 315                 320 


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
                325                 330                 335     


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
            340                 345                 350         


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
        355                 360                 365             


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
    370                 375                 380                 


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
385                 390                 395                 400 


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
                405                 410                 415     


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
            420                 425                 430         


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
        435                 440                 445             


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
    450                 455                 460                 


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
465                 470                 475                 480 


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
                485                 490                 495     


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
            500                 505                 510         


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
        515                 520                 525             


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
    530                 535                 540                 


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
545                 550                 555                 560 


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
                565                 570                 575     


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
            580                 585                 590         


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
        595                 600                 605             


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
    610                 615                 620                 


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
625                 630                 635                 640 


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
                645                 650                 655     


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
            660                 665                 670         


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
        675                 680                 685             


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
    690                 695                 700                 


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
705                 710                 715                 720 


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
                725                 730                 735     


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
            740                 745                 750         


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
        755                 760                 765             


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
    770                 775                 780                 


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
785                 790                 795                 800 


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
                805                 810                 815     


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
            820                 825                 830         


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
        835                 840                 845             


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
    850                 855                 860                 


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
865                 870                 875                 880 


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
                885                 890                 895     


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
            900                 905                 910         


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
        915                 920                 925             


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
    930                 935                 940                 


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
945                 950                 955                 960 


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
                965                 970                 975     


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
            980                 985                 990         


Ile Glu Glu Gly Ile Lys Glu Leu  Gly Ser Gln Ile Leu  Lys Glu His 
        995                 1000                 1005             


Pro Val  Glu Asn Thr Gln Leu  Gln Asn Glu Lys Leu  Tyr Leu Tyr 
    1010                 1015                 1020             


Tyr Leu  Gln Asn Gly Arg Asp  Met Tyr Val Asp Gln  Glu Leu Asp 
    1025                 1030                 1035             


Ile Asn  Arg Leu Ser Asp Tyr  Asp Val Asp His Ile  Val Pro Gln 
    1040                 1045                 1050             


Ser Phe  Leu Lys Asp Asp Ser  Ile Asp Asn Lys Val  Leu Thr Arg 
    1055                 1060                 1065             


Ser Asp  Lys Asn Arg Gly Lys  Ser Asp Asn Val Pro  Ser Glu Glu 
    1070                 1075                 1080             


Val Val  Lys Lys Met Lys Asn  Tyr Trp Arg Gln Leu  Leu Asn Ala 
    1085                 1090                 1095             


Lys Leu  Ile Thr Gln Arg Lys  Phe Asp Asn Leu Thr  Lys Ala Glu 
    1100                 1105                 1110             


Arg Gly  Gly Leu Ser Glu Leu  Asp Lys Ala Gly Phe  Ile Lys Arg 
    1115                 1120                 1125             


Gln Leu  Val Glu Thr Arg Gln  Ile Thr Lys His Val  Ala Gln Ile 
    1130                 1135                 1140             


Leu Asp  Ser Arg Met Asn Thr  Lys Tyr Asp Glu Asn  Asp Lys Leu 
    1145                 1150                 1155             


Ile Arg  Glu Val Lys Val Ile  Thr Leu Lys Ser Lys  Leu Val Ser 
    1160                 1165                 1170             


Asp Phe  Arg Lys Asp Phe Gln  Phe Tyr Lys Val Arg  Glu Ile Asn 
    1175                 1180                 1185             


Asn Tyr  His His Ala His Asp  Ala Tyr Leu Asn Ala  Val Val Gly 
    1190                 1195                 1200             


Thr Ala  Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
    1205                 1210                 1215             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1220                 1225                 1230             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1235                 1240                 1245             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1250                 1255                 1260             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1265                 1270                 1275             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1280                 1285                 1290             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1295                 1300                 1305             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1310                 1315                 1320             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1325                 1330                 1335             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1340                 1345                 1350             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1355                 1360                 1365             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1370                 1375                 1380             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1385                 1390                 1395             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1400                 1405                 1410             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1415                 1420                 1425             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1430                 1435                 1440             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1445                 1450                 1455             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1460                 1465                 1470             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1475                 1480                 1485             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1490                 1495                 1500             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1505                 1510                 1515             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1520                 1525                 1530             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1535                 1540                 1545             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1550                 1555                 1560             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp Gly 
    1565                 1570                 1575             


Ser Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1580                 1585                 


<210> 478
<211> 1361
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)

<400> 478
Met Gly Ser Ser His His His His His His Gly Met Ser Ser Glu Thr 
1               5                   10                  15      


Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His 
            20                  25                  30          


Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys 
        35                  40                  45              


Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His 
    50                  55                  60                  


Thr Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys 
65                  70                  75                  80  


Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr 
                85                  90                  95      


Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr 
            100                 105                 110         


Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala 
        115                 120                 125             


Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp 
    130                 135                 140                 


Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser 
145                 150                 155                 160 


Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala 
                165                 170                 175     


His Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu 
            180                 185                 190         


Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg 
        195                 200                 205             


Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys 
    210                 215                 220                 


His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
225                 230                 235                 240 


Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
                245                 250                 255     


Gly Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly 
            260                 265                 270         


Trp Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu 
        275                 280                 285             


Gly Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly 
    290                 295                 300                 


Ala Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val 
305                 310                 315                 320 


Ile Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys 
                325                 330                 335     


Lys His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val 
            340                 345                 350         


Pro Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu 
        355                 360                 365             


Lys Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys 
    370                 375                 380                 


His Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp 
385                 390                 395                 400 


Glu Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys 
                405                 410                 415     


Asn Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg 
            420                 425                 430         


Lys Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg 
        435                 440                 445             


Ala Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn 
    450                 455                 460                 


Leu Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile 
465                 470                 475                 480 


Leu Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser 
                485                 490                 495     


Pro Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu 
            500                 505                 510         


Glu Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met 
        515                 520                 525             


Ala Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg 
    530                 535                 540                 


Gly Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp 
545                 550                 555                 560 


Leu Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val 
                565                 570                 575     


Leu Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu 
            580                 585                 590         


Lys Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu 
        595                 600                 605             


Lys Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala 
    610                 615                 620                 


Leu Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln 
625                 630                 635                 640 


Leu Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr 
                645                 650                 655     


Ile Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu 
            660                 665                 670         


Ala Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser 
        675                 680                 685             


Leu Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val 
    690                 695                 700                 


Tyr Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser 
705                 710                 715                 720 


Leu Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile 
                725                 730                 735     


Ser Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn 
            740                 745                 750         


Pro Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe 
        755                 760                 765             


Arg Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys 
    770                 775                 780                 


Asn Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg 
785                 790                 795                 800 


Pro Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn 
                805                 810                 815     


Gly Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu 
            820                 825                 830         


Lys Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg 
        835                 840                 845             


Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu 
    850                 855                 860                 


Asn Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala 
865                 870                 875                 880 


Pro Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg 
                885                 890                 895     


Asn Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr 
            900                 905                 910         


Thr Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys 
    930                 935                 940                 


Gly Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val 
945                 950                 955                 960 


Thr Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn 
                965                 970                 975     


Gly Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr 
            980                 985                 990         


Gln Gly Ile Val Asn Lys Val Ser  Arg Tyr Ser Lys Ser  Arg Glu Leu 
        995                 1000                 1005             


Trp Asp  Tyr Glu Val Asp Met  Glu Thr Gly Glu Val  Leu Gln Lys 
    1010                 1015                 1020             


Lys Asn  Lys Asn Thr Lys Asp  Val Phe Pro Glu Pro  Trp Leu Asn 
    1025                 1030                 1035             


Phe Arg  Tyr Glu Leu Glu Gln  Lys Val Arg Val Arg  Pro Leu Asp 
    1040                 1045                 1050             


Ile Pro  Glu Thr Ala Asp Ile  Thr Glu Met Glu Glu  Pro Phe Val 
    1055                 1060                 1065             


Ser His  Met Pro Asn Arg Lys  Ile His Gly Pro Ala  His Lys Glu 
    1070                 1075                 1080             


Thr Ile  Arg Ser Gly Arg Leu  Lys Glu Glu Gly Tyr  Thr Ile Ser 
    1085                 1090                 1095             


Lys Thr  Ala Leu Ile Asp Leu  Lys Leu Thr Glu Asp  Lys Glu Glu 
    1100                 1105                 1110             


Ile Lys  Gly Tyr Tyr Asn Lys  Glu Ser Asp Arg Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Ala Leu  Lys Lys Gln Leu Gln  Arg Tyr Gly Gly Lys  Ala Lys Glu 
    1130                 1135                 1140             


Ala Phe  Lys Glu Pro Phe His  Lys Pro Lys Ala Asp  Gly Thr Pro 
    1145                 1150                 1155             


Gly Pro  Ile Val Asn Lys Val  Lys Ile Met Glu Lys  Ser Thr Met 
    1160                 1165                 1170             


Leu Ile  Pro Val Asn Gly Gly  Lys Gly Leu Ala Ser  Asn Gly Asn 
    1175                 1180                 1185             


Met Val  Arg Ile Asp Val Phe  Arg Ala Glu Glu Lys  Gly Lys Lys 
    1190                 1195                 1200             


Lys Tyr  Tyr Phe Ile Pro Val  Tyr Val Ala Asp Thr  Val Lys Glu 
    1205                 1210                 1215             


Glu Leu  Pro Asn Arg Ala Val  Leu Ala His Lys Pro  Tyr Glu Ala 
    1220                 1225                 1230             


Trp Lys  Ile Met Lys Glu Glu  Asn Phe Ile Phe Ser  Leu Tyr Pro 
    1235                 1240                 1245             


Asn Asp  Leu Ile Phe Val Asp  Ala Gly Lys Glu Ile  Pro Phe Lys 
    1250                 1255                 1260             


Ala Ala  Leu Lys Gly Ser Thr  Leu Asp Pro Glu Lys  Lys Ala Ser 
    1265                 1270                 1275             


Arg Phe  Leu Met Tyr Tyr Lys  Gly Ala Asp Ile Ala  Thr Gly Ser 
    1280                 1285                 1290             


Ile Ser  Gly Val Asn His Asp  Glu Thr Tyr Lys Ala  Arg Gly Val 
    1295                 1300                 1305             


Gly Ile  Gln Ser Leu Arg Glu  Ile Lys Lys Cys Cys  Ile Asp Val 
    1310                 1315                 1320             


Leu Gly  Asn Ile Ser Phe Ala  Ser Lys Glu Lys Arg  Gln Thr Phe 
    1325                 1330                 1335             


Arg Ser  Gly Gly Ser Ser Ser  Gly Gly Gly Ala Ser  Ser Gly Gly 
    1340                 1345                 1350             


Ser Pro  Lys Lys Lys Arg Lys  Val 
    1355                 1360     


<210> 479
<211> 1441
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-UGI (PBS1)

<400> 479
Met Gly Ser Ser His His His His His His Gly Met Ser Ser Glu Thr 
1               5                   10                  15      


Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His 
            20                  25                  30          


Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys 
        35                  40                  45              


Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His 
    50                  55                  60                  


Thr Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys 
65                  70                  75                  80  


Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr 
                85                  90                  95      


Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr 
            100                 105                 110         


Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala 
        115                 120                 125             


Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp 
    130                 135                 140                 


Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser 
145                 150                 155                 160 


Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala 
                165                 170                 175     


His Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu 
            180                 185                 190         


Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg 
        195                 200                 205             


Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys 
    210                 215                 220                 


His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
225                 230                 235                 240 


Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
                245                 250                 255     


Gly Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly 
            260                 265                 270         


Trp Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu 
        275                 280                 285             


Gly Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly 
    290                 295                 300                 


Ala Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val 
305                 310                 315                 320 


Ile Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys 
                325                 330                 335     


Lys His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val 
            340                 345                 350         


Pro Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu 
        355                 360                 365             


Lys Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys 
    370                 375                 380                 


His Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp 
385                 390                 395                 400 


Glu Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys 
                405                 410                 415     


Asn Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg 
            420                 425                 430         


Lys Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg 
        435                 440                 445             


Ala Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn 
    450                 455                 460                 


Leu Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile 
465                 470                 475                 480 


Leu Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser 
                485                 490                 495     


Pro Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu 
            500                 505                 510         


Glu Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met 
        515                 520                 525             


Ala Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg 
    530                 535                 540                 


Gly Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp 
545                 550                 555                 560 


Leu Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val 
                565                 570                 575     


Leu Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu 
            580                 585                 590         


Lys Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu 
        595                 600                 605             


Lys Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala 
    610                 615                 620                 


Leu Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln 
625                 630                 635                 640 


Leu Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr 
                645                 650                 655     


Ile Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu 
            660                 665                 670         


Ala Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser 
        675                 680                 685             


Leu Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val 
    690                 695                 700                 


Tyr Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser 
705                 710                 715                 720 


Leu Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile 
                725                 730                 735     


Ser Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn 
            740                 745                 750         


Pro Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe 
        755                 760                 765             


Arg Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys 
    770                 775                 780                 


Asn Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg 
785                 790                 795                 800 


Pro Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn 
                805                 810                 815     


Gly Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu 
            820                 825                 830         


Lys Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg 
        835                 840                 845             


Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu 
    850                 855                 860                 


Asn Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala 
865                 870                 875                 880 


Pro Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg 
                885                 890                 895     


Asn Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr 
            900                 905                 910         


Thr Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys 
    930                 935                 940                 


Gly Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val 
945                 950                 955                 960 


Thr Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn 
                965                 970                 975     


Gly Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr 
            980                 985                 990         


Gln Gly Ile Val Asn Lys Val Ser  Arg Tyr Ser Lys Ser  Arg Glu Leu 
        995                 1000                 1005             


Trp Asp  Tyr Glu Val Asp Met  Glu Thr Gly Glu Val  Leu Gln Lys 
    1010                 1015                 1020             


Lys Asn  Lys Asn Thr Lys Asp  Val Phe Pro Glu Pro  Trp Leu Asn 
    1025                 1030                 1035             


Phe Arg  Tyr Glu Leu Glu Gln  Lys Val Arg Val Arg  Pro Leu Asp 
    1040                 1045                 1050             


Ile Pro  Glu Thr Ala Asp Ile  Thr Glu Met Glu Glu  Pro Phe Val 
    1055                 1060                 1065             


Ser His  Met Pro Asn Arg Lys  Ile His Gly Pro Ala  His Lys Glu 
    1070                 1075                 1080             


Thr Ile  Arg Ser Gly Arg Leu  Lys Glu Glu Gly Tyr  Thr Ile Ser 
    1085                 1090                 1095             


Lys Thr  Ala Leu Ile Asp Leu  Lys Leu Thr Glu Asp  Lys Glu Glu 
    1100                 1105                 1110             


Ile Lys  Gly Tyr Tyr Asn Lys  Glu Ser Asp Arg Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Ala Leu  Lys Lys Gln Leu Gln  Arg Tyr Gly Gly Lys  Ala Lys Glu 
    1130                 1135                 1140             


Ala Phe  Lys Glu Pro Phe His  Lys Pro Lys Ala Asp  Gly Thr Pro 
    1145                 1150                 1155             


Gly Pro  Ile Val Asn Lys Val  Lys Ile Met Glu Lys  Ser Thr Met 
    1160                 1165                 1170             


Leu Ile  Pro Val Asn Gly Gly  Lys Gly Leu Ala Ser  Asn Gly Asn 
    1175                 1180                 1185             


Met Val  Arg Ile Asp Val Phe  Arg Ala Glu Glu Lys  Gly Lys Lys 
    1190                 1195                 1200             


Lys Tyr  Tyr Phe Ile Pro Val  Tyr Val Ala Asp Thr  Val Lys Glu 
    1205                 1210                 1215             


Glu Leu  Pro Asn Arg Ala Val  Leu Ala His Lys Pro  Tyr Glu Ala 
    1220                 1225                 1230             


Trp Lys  Ile Met Lys Glu Glu  Asn Phe Ile Phe Ser  Leu Tyr Pro 
    1235                 1240                 1245             


Asn Asp  Leu Ile Phe Val Asp  Ala Gly Lys Glu Ile  Pro Phe Lys 
    1250                 1255                 1260             


Ala Ala  Leu Lys Gly Ser Thr  Leu Asp Pro Glu Lys  Lys Ala Ser 
    1265                 1270                 1275             


Arg Phe  Leu Met Tyr Tyr Lys  Gly Ala Asp Ile Ala  Thr Gly Ser 
    1280                 1285                 1290             


Ile Ser  Gly Val Asn His Asp  Glu Thr Tyr Lys Ala  Arg Gly Val 
    1295                 1300                 1305             


Gly Ile  Gln Ser Leu Arg Glu  Ile Lys Lys Cys Cys  Ile Asp Val 
    1310                 1315                 1320             


Leu Gly  Asn Ile Ser Phe Ala  Ser Lys Glu Lys Arg  Gln Thr Phe 
    1325                 1330                 1335             


Arg Ser  Gly Gly Ser Ser Arg  Thr Asn Leu Ser Asp  Ile Ile Glu 
    1340                 1345                 1350             


Lys Glu  Thr Gly Lys Gln Leu  Val Ile Gln Glu Ser  Ile Leu Met 
    1355                 1360                 1365             


Leu Pro  Glu Glu Val Glu Glu  Val Ile Gly Asn Lys  Pro Glu Ser 
    1370                 1375                 1380             


Asp Ile  Leu Val His Thr Ala  Tyr Asp Glu Ser Thr  Asp Glu Asn 
    1385                 1390                 1395             


Val Met  Leu Leu Thr Ser Asp  Ala Pro Glu Tyr Lys  Pro Trp Ala 
    1400                 1405                 1410             


Leu Val  Ile Gln Asp Ser Asn  Gly Glu Asn Lys Ile  Lys Met Leu 
    1415                 1420                 1425             


Ala Ser  Ser Gly Gly Ser Pro  Lys Lys Lys Arg Lys  Val 
    1430                 1435                 1440     


<210> 480
<211> 1442
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-1

<400> 480
Met Gly Ser Ser His His His His His His Gly Met Ser Ser Glu Thr 
1               5                   10                  15      


Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His 
            20                  25                  30          


Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys 
        35                  40                  45              


Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His 
    50                  55                  60                  


Thr Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys 
65                  70                  75                  80  


Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr 
                85                  90                  95      


Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr 
            100                 105                 110         


Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala 
        115                 120                 125             


Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp 
    130                 135                 140                 


Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser 
145                 150                 155                 160 


Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala 
                165                 170                 175     


His Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu 
            180                 185                 190         


Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg 
        195                 200                 205             


Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys 
    210                 215                 220                 


His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
225                 230                 235                 240 


Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
                245                 250                 255     


Gly Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly 
            260                 265                 270         


Trp Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu 
        275                 280                 285             


Gly Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly 
    290                 295                 300                 


Ala Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val 
305                 310                 315                 320 


Ile Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys 
                325                 330                 335     


Lys His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val 
            340                 345                 350         


Pro Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu 
        355                 360                 365             


Lys Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys 
    370                 375                 380                 


His Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp 
385                 390                 395                 400 


Glu Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys 
                405                 410                 415     


Asn Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg 
            420                 425                 430         


Lys Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg 
        435                 440                 445             


Ala Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn 
    450                 455                 460                 


Leu Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile 
465                 470                 475                 480 


Leu Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser 
                485                 490                 495     


Pro Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu 
            500                 505                 510         


Glu Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met 
        515                 520                 525             


Ala Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg 
    530                 535                 540                 


Gly Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp 
545                 550                 555                 560 


Leu Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val 
                565                 570                 575     


Leu Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu 
            580                 585                 590         


Lys Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu 
        595                 600                 605             


Lys Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala 
    610                 615                 620                 


Leu Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln 
625                 630                 635                 640 


Leu Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr 
                645                 650                 655     


Ile Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu 
            660                 665                 670         


Ala Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser 
        675                 680                 685             


Leu Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val 
    690                 695                 700                 


Tyr Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser 
705                 710                 715                 720 


Leu Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile 
                725                 730                 735     


Ser Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn 
            740                 745                 750         


Pro Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe 
        755                 760                 765             


Arg Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys 
    770                 775                 780                 


Asn Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg 
785                 790                 795                 800 


Pro Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn 
                805                 810                 815     


Gly Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu 
            820                 825                 830         


Lys Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg 
        835                 840                 845             


Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu 
    850                 855                 860                 


Asn Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala 
865                 870                 875                 880 


Pro Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg 
                885                 890                 895     


Asn Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr 
            900                 905                 910         


Thr Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys 
    930                 935                 940                 


Gly Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val 
945                 950                 955                 960 


Thr Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn 
                965                 970                 975     


Gly Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr 
            980                 985                 990         


Gln Gly Ile Val Asn Lys Val Ser  Arg Tyr Ser Lys Ser  Arg Glu Leu 
        995                 1000                 1005             


Trp Asp  Tyr Glu Val Asp Met  Glu Thr Gly Glu Val  Leu Gln Lys 
    1010                 1015                 1020             


Lys Asn  Lys Asn Thr Lys Asp  Val Phe Pro Glu Pro  Trp Leu Asn 
    1025                 1030                 1035             


Phe Arg  Tyr Glu Leu Glu Gln  Lys Val Arg Val Arg  Pro Leu Asp 
    1040                 1045                 1050             


Ile Pro  Glu Thr Ala Asp Ile  Thr Glu Met Glu Glu  Pro Phe Val 
    1055                 1060                 1065             


Ser His  Met Pro Asn Arg Lys  Ile His Gly Pro Ala  His Lys Glu 
    1070                 1075                 1080             


Thr Ile  Arg Ser Gly Arg Leu  Lys Glu Glu Gly Tyr  Thr Ile Ser 
    1085                 1090                 1095             


Lys Thr  Ala Leu Ile Asp Leu  Lys Leu Thr Glu Asp  Lys Glu Glu 
    1100                 1105                 1110             


Ile Lys  Gly Tyr Tyr Asn Lys  Glu Ser Asp Arg Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Ala Leu  Lys Lys Gln Leu Gln  Arg Tyr Gly Gly Lys  Ala Lys Glu 
    1130                 1135                 1140             


Ala Phe  Lys Glu Pro Phe His  Lys Pro Lys Ala Asp  Gly Thr Pro 
    1145                 1150                 1155             


Gly Pro  Ile Val Asn Lys Val  Lys Ile Met Glu Lys  Ser Thr Met 
    1160                 1165                 1170             


Leu Ile  Pro Val Asn Gly Gly  Lys Gly Leu Ala Ser  Asn Gly Asn 
    1175                 1180                 1185             


Met Val  Arg Ile Asp Val Phe  Arg Ala Glu Glu Lys  Gly Lys Lys 
    1190                 1195                 1200             


Lys Tyr  Tyr Phe Ile Pro Val  Tyr Val Ala Asp Thr  Val Lys Glu 
    1205                 1210                 1215             


Glu Leu  Pro Asn Arg Ala Val  Leu Ala His Lys Pro  Tyr Glu Ala 
    1220                 1225                 1230             


Trp Lys  Ile Met Lys Glu Glu  Asn Phe Ile Phe Ser  Leu Tyr Pro 
    1235                 1240                 1245             


Asn Asp  Leu Ile Phe Val Asp  Ala Gly Lys Glu Ile  Pro Phe Lys 
    1250                 1255                 1260             


Ala Ala  Leu Lys Gly Ser Thr  Leu Asp Pro Glu Lys  Lys Ala Ser 
    1265                 1270                 1275             


Arg Phe  Leu Met Tyr Tyr Lys  Gly Ala Asp Ile Ala  Thr Gly Ser 
    1280                 1285                 1290             


Ile Ser  Gly Val Asn His Asp  Glu Thr Tyr Lys Ala  Arg Gly Val 
    1295                 1300                 1305             


Gly Ile  Gln Ser Leu Arg Glu  Ile Lys Lys Cys Cys  Ile Asp Val 
    1310                 1315                 1320             


Leu Gly  Asn Ile Ser Phe Ala  Ser Lys Glu Lys Arg  Gln Thr Phe 
    1325                 1330                 1335             


Arg Ser  Gly Gly Ser Ser Ser  Lys Thr Ile Lys Asp  Leu Glu Glu 
    1340                 1345                 1350             


Phe Ala  Ala Glu Lys Leu Gly  Tyr Asp Ile Val Phe  Gln Glu Ser 
    1355                 1360                 1365             


Ile Ser  Met Glu Pro Gln Glu  Val Glu His Leu Ile  Thr Thr Asp 
    1370                 1375                 1380             


Glu Lys  Ser Val Leu Val Asn  Ser Thr Tyr Asp Asp  Asn Lys Glu 
    1385                 1390                 1395             


Ile Thr  Ile Ser Met Ile Ser  Thr Pro His Pro Glu  Tyr Arg Pro 
    1400                 1405                 1410             


Ile Ala  Leu Ile Ile Gln Asp  Met Glu Gly Glu Val  Gln Arg Lys 
    1415                 1420                 1425             


Glu Leu  Gly Ser Gly Gly Ser  Pro Lys Lys Lys Arg  Lys Val 
    1430                 1435                 1440         


<210> 481
<211> 1443
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-2

<400> 481
Met Gly Ser Ser His His His His His His Gly Met Ser Ser Glu Thr 
1               5                   10                  15      


Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His 
            20                  25                  30          


Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys 
        35                  40                  45              


Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His 
    50                  55                  60                  


Thr Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys 
65                  70                  75                  80  


Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr 
                85                  90                  95      


Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr 
            100                 105                 110         


Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala 
        115                 120                 125             


Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp 
    130                 135                 140                 


Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser 
145                 150                 155                 160 


Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala 
                165                 170                 175     


His Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu 
            180                 185                 190         


Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg 
        195                 200                 205             


Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys 
    210                 215                 220                 


His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
225                 230                 235                 240 


Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
                245                 250                 255     


Gly Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly 
            260                 265                 270         


Trp Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu 
        275                 280                 285             


Gly Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly 
    290                 295                 300                 


Ala Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val 
305                 310                 315                 320 


Ile Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys 
                325                 330                 335     


Lys His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val 
            340                 345                 350         


Pro Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu 
        355                 360                 365             


Lys Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys 
    370                 375                 380                 


His Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp 
385                 390                 395                 400 


Glu Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys 
                405                 410                 415     


Asn Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg 
            420                 425                 430         


Lys Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg 
        435                 440                 445             


Ala Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn 
    450                 455                 460                 


Leu Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile 
465                 470                 475                 480 


Leu Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser 
                485                 490                 495     


Pro Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu 
            500                 505                 510         


Glu Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met 
        515                 520                 525             


Ala Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg 
    530                 535                 540                 


Gly Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp 
545                 550                 555                 560 


Leu Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val 
                565                 570                 575     


Leu Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu 
            580                 585                 590         


Lys Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu 
        595                 600                 605             


Lys Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala 
    610                 615                 620                 


Leu Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln 
625                 630                 635                 640 


Leu Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr 
                645                 650                 655     


Ile Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu 
            660                 665                 670         


Ala Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser 
        675                 680                 685             


Leu Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val 
    690                 695                 700                 


Tyr Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser 
705                 710                 715                 720 


Leu Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile 
                725                 730                 735     


Ser Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn 
            740                 745                 750         


Pro Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe 
        755                 760                 765             


Arg Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys 
    770                 775                 780                 


Asn Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg 
785                 790                 795                 800 


Pro Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn 
                805                 810                 815     


Gly Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu 
            820                 825                 830         


Lys Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg 
        835                 840                 845             


Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu 
    850                 855                 860                 


Asn Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala 
865                 870                 875                 880 


Pro Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg 
                885                 890                 895     


Asn Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr 
            900                 905                 910         


Thr Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys 
    930                 935                 940                 


Gly Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val 
945                 950                 955                 960 


Thr Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn 
                965                 970                 975     


Gly Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr 
            980                 985                 990         


Gln Gly Ile Val Asn Lys Val Ser  Arg Tyr Ser Lys Ser  Arg Glu Leu 
        995                 1000                 1005             


Trp Asp  Tyr Glu Val Asp Met  Glu Thr Gly Glu Val  Leu Gln Lys 
    1010                 1015                 1020             


Lys Asn  Lys Asn Thr Lys Asp  Val Phe Pro Glu Pro  Trp Leu Asn 
    1025                 1030                 1035             


Phe Arg  Tyr Glu Leu Glu Gln  Lys Val Arg Val Arg  Pro Leu Asp 
    1040                 1045                 1050             


Ile Pro  Glu Thr Ala Asp Ile  Thr Glu Met Glu Glu  Pro Phe Val 
    1055                 1060                 1065             


Ser His  Met Pro Asn Arg Lys  Ile His Gly Pro Ala  His Lys Glu 
    1070                 1075                 1080             


Thr Ile  Arg Ser Gly Arg Leu  Lys Glu Glu Gly Tyr  Thr Ile Ser 
    1085                 1090                 1095             


Lys Thr  Ala Leu Ile Asp Leu  Lys Leu Thr Glu Asp  Lys Glu Glu 
    1100                 1105                 1110             


Ile Lys  Gly Tyr Tyr Asn Lys  Glu Ser Asp Arg Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Ala Leu  Lys Lys Gln Leu Gln  Arg Tyr Gly Gly Lys  Ala Lys Glu 
    1130                 1135                 1140             


Ala Phe  Lys Glu Pro Phe His  Lys Pro Lys Ala Asp  Gly Thr Pro 
    1145                 1150                 1155             


Gly Pro  Ile Val Asn Lys Val  Lys Ile Met Glu Lys  Ser Thr Met 
    1160                 1165                 1170             


Leu Ile  Pro Val Asn Gly Gly  Lys Gly Leu Ala Ser  Asn Gly Asn 
    1175                 1180                 1185             


Met Val  Arg Ile Asp Val Phe  Arg Ala Glu Glu Lys  Gly Lys Lys 
    1190                 1195                 1200             


Lys Tyr  Tyr Phe Ile Pro Val  Tyr Val Ala Asp Thr  Val Lys Glu 
    1205                 1210                 1215             


Glu Leu  Pro Asn Arg Ala Val  Leu Ala His Lys Pro  Tyr Glu Ala 
    1220                 1225                 1230             


Trp Lys  Ile Met Lys Glu Glu  Asn Phe Ile Phe Ser  Leu Tyr Pro 
    1235                 1240                 1245             


Asn Asp  Leu Ile Phe Val Asp  Ala Gly Lys Glu Ile  Pro Phe Lys 
    1250                 1255                 1260             


Ala Ala  Leu Lys Gly Ser Thr  Leu Asp Pro Glu Lys  Lys Ala Ser 
    1265                 1270                 1275             


Arg Phe  Leu Met Tyr Tyr Lys  Gly Ala Asp Ile Ala  Thr Gly Ser 
    1280                 1285                 1290             


Ile Ser  Gly Val Asn His Asp  Glu Thr Tyr Lys Ala  Arg Gly Val 
    1295                 1300                 1305             


Gly Ile  Gln Ser Leu Arg Glu  Ile Lys Lys Cys Cys  Ile Asp Val 
    1310                 1315                 1320             


Leu Gly  Asn Ile Ser Phe Ala  Ser Lys Glu Lys Arg  Gln Thr Phe 
    1325                 1330                 1335             


Arg Ser  Gly Gly Ser Ser Ser  Lys Thr Ile Lys Asp  Leu Glu Glu 
    1340                 1345                 1350             


Phe Ala  Ala Glu Lys Leu Gly  Tyr Asp Ile Val Phe  Gln Glu Ser 
    1355                 1360                 1365             


Ile Ser  Met Glu Pro Gln Glu  Val Glu His Leu Ile  Thr Thr Asp 
    1370                 1375                 1380             


Glu Lys  Ser Val Leu Val Asn  Ser Thr Tyr Asp Asp  Asp Lys Glu 
    1385                 1390                 1395             


Ile Thr  Ile Ser Val Ile Ser  Thr Pro His Pro Glu  Tyr Arg Pro 
    1400                 1405                 1410             


Ile Ala  Leu Val Ile Gln Asp  Met Glu Gly Glu Val  Gln Arg Lys 
    1415                 1420                 1425             


Glu Leu  Tyr Gly Ser Gly Gly  Ser Pro Lys Lys Lys  Arg Lys Val 
    1430                 1435                 1440             


<210> 482
<211> 1422
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-

<400> 482
Met Gly Ser Ser His His His His His His Gly Met Ser Ser Glu Thr 
1               5                   10                  15      


Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His 
            20                  25                  30          


Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys 
        35                  40                  45              


Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His 
    50                  55                  60                  


Thr Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys 
65                  70                  75                  80  


Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr 
                85                  90                  95      


Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr 
            100                 105                 110         


Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala 
        115                 120                 125             


Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp 
    130                 135                 140                 


Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser 
145                 150                 155                 160 


Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala 
                165                 170                 175     


His Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu 
            180                 185                 190         


Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg 
        195                 200                 205             


Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys 
    210                 215                 220                 


His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys 
225                 230                 235                 240 


Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
                245                 250                 255     


Gly Met Asn Tyr Val Leu Gly Leu Ala Val Gly Ile Ala Ser Val Gly 
            260                 265                 270         


Trp Ala Val Leu Glu Leu Asn Glu Glu Asp Asn Pro Ile Arg Ile Glu 
        275                 280                 285             


Gly Leu Gly Ala Arg Ile Phe Asp Lys Ala Glu Val Pro Lys Thr Gly 
    290                 295                 300                 


Ala Ser Leu Ala Ala Pro Arg Arg Met Ser Arg Gly Ile Arg Arg Val 
305                 310                 315                 320 


Ile Arg Arg Arg Arg Phe Arg Leu Gln Arg Val Arg Ser Tyr Leu Lys 
                325                 330                 335     


Lys His Asn Ile Leu Pro Ala Glu Lys Val Asp His Leu Tyr Asp Val 
            340                 345                 350         


Pro Ser Ala Ile Asp Ile Tyr Glu Leu Arg Lys Arg Ala Leu Thr Glu 
        355                 360                 365             


Lys Val Thr Ala Glu Glu Trp Ala Arg Leu Leu Ile Phe Phe Ala Lys 
    370                 375                 380                 


His Arg Gly Phe Lys Ser Asn Arg Lys Lys Ala Ser Gly Asp Ala Asp 
385                 390                 395                 400 


Glu Gly Glu Met Leu Lys Ala Ile Ala Ala Asn Ala Glu Ile Leu Lys 
                405                 410                 415     


Asn Tyr Arg Thr Val Gly Glu Met Leu Cys Asp Asn Glu Lys Phe Arg 
            420                 425                 430         


Lys Arg Lys Arg Asn Arg Asp Gly Ala Tyr Asn Phe Thr Val Ser Arg 
        435                 440                 445             


Ala Met Leu Met Glu Glu Ile His Thr Leu Phe Glu Ile Gln Arg Asn 
    450                 455                 460                 


Leu Gly Gln Lys Phe Ala Asp Glu Lys Leu Glu Glu Glu Tyr Val Ile 
465                 470                 475                 480 


Leu Phe Ala Ala Gln Arg Lys Phe Asp Glu Gly Pro Gly Glu Asn Ser 
                485                 490                 495     


Pro Tyr Ala Gly Asn Gln Ile Glu Lys Met Ile Gly Ser Cys Thr Leu 
            500                 505                 510         


Glu Gly Lys Lys Glu Lys Arg Ala Pro Lys Ala Ser Tyr Ala Phe Met 
        515                 520                 525             


Ala Phe Asn Leu Trp Gln Lys Ile Asn His Leu Lys Val Asn Arg Arg 
    530                 535                 540                 


Gly Ser Glu Arg Phe Leu Thr Glu Glu Glu Arg Arg Arg Ile Ala Asp 
545                 550                 555                 560 


Leu Ala Trp Lys Lys Glu Lys Leu Thr Tyr Gly Ser Leu Arg Lys Val 
                565                 570                 575     


Leu Ser Leu Asp Pro Glu Asp Arg Phe Val Gly Leu Arg Tyr Asp Leu 
            580                 585                 590         


Lys Lys Gly Lys Ala Glu Asn Leu Glu Glu Pro Val Ser Glu Ala Glu 
        595                 600                 605             


Lys Arg Ser Phe Ser Trp Val Lys Asp Tyr His Ser Ile Arg Lys Ala 
    610                 615                 620                 


Leu Asp Lys Val Glu Lys Asn Arg Ile Leu Lys Leu Ser His Glu Gln 
625                 630                 635                 640 


Leu Asp Thr Ile Ala Thr Ala Phe Ser Val Tyr Lys Asn Glu Glu Thr 
                645                 650                 655     


Ile Glu Lys Tyr Leu Glu Glu Gly Gly Ile Gly Lys Ala Asp Gly Glu 
            660                 665                 670         


Ala Leu Leu Glu Arg Leu Ser Gly Phe Ser Lys Phe Gly His Leu Ser 
        675                 680                 685             


Leu Lys Ala Cys Tyr Lys Leu Leu Pro Phe Leu Glu Lys Gly Glu Val 
    690                 695                 700                 


Tyr Ser Arg Ala Cys Glu Leu Ala Gly Tyr Asp Phe Ser Lys Ser Ser 
705                 710                 715                 720 


Leu Glu Asn Ile Asp Asp Ile Pro Asn Pro Val Val Lys Arg Ser Ile 
                725                 730                 735     


Ser Gln Thr Leu Lys Val Ile Asn Ala Ile Ile Leu Arg Tyr Gly Asn 
            740                 745                 750         


Pro Val Glu Val His Ile Glu Leu Ala Arg Glu Leu Ala Arg Ser Phe 
        755                 760                 765             


Arg Asp Arg Lys Lys Met Glu Lys Gly Met Gln Glu Asn Gln Ala Lys 
    770                 775                 780                 


Asn Glu Lys Ile Arg Gln Arg Leu Ile Ser Glu Phe Gly Val Leu Arg 
785                 790                 795                 800 


Pro Lys Gly Met Asp Ile Val Lys Phe Lys Leu Cys Glu Glu Gln Asn 
                805                 810                 815     


Gly Glu Cys Ala Tyr Ser Gln Lys His Phe Asp Met Glu Lys Leu Leu 
            820                 825                 830         


Lys Asp Pro Ser Tyr Ala Glu Val Asp His Ile Ile Pro Tyr Ser Arg 
        835                 840                 845             


Ser Phe Asp Asp Ser Tyr Asn Asn Lys Val Leu Val Leu Thr Lys Glu 
    850                 855                 860                 


Asn Arg Glu Lys Arg Asn Arg Ile Pro Met Glu Tyr Leu Ala Asp Ala 
865                 870                 875                 880 


Pro Glu Arg Lys Asn Arg Phe Ile His Trp Val Lys Ser Thr Ile Arg 
                885                 890                 895     


Asn Ser Arg Lys Arg Glu Asn Leu Leu Arg Thr Asp Tyr Thr Ala Thr 
            900                 905                 910         


Thr Glu Asn Glu Trp Lys Arg Arg Asn Leu Gln Asp Thr Gln Tyr Ile 
        915                 920                 925             


Ser Lys Tyr Leu Tyr Asn Tyr Leu Arg His His Leu Val Leu Ala Lys 
    930                 935                 940                 


Gly Tyr Thr Glu Arg Lys Arg Arg Ile Ile Pro Val Asn Gly Ala Val 
945                 950                 955                 960 


Thr Ala Tyr Phe Arg Lys Arg Leu Gly Ile Asn Lys Ile Arg Glu Asn 
                965                 970                 975     


Gly Asp Leu His His Ala Val Asp Ala Val Ile Ile Ala Cys Ala Thr 
            980                 985                 990         


Gln Gly Ile Val Asn Lys Val Ser  Arg Tyr Ser Lys Ser  Arg Glu Leu 
        995                 1000                 1005             


Trp Asp  Tyr Glu Val Asp Met  Glu Thr Gly Glu Val  Leu Gln Lys 
    1010                 1015                 1020             


Lys Asn  Lys Asn Thr Lys Asp  Val Phe Pro Glu Pro  Trp Leu Asn 
    1025                 1030                 1035             


Phe Arg  Tyr Glu Leu Glu Gln  Lys Val Arg Val Arg  Pro Leu Asp 
    1040                 1045                 1050             


Ile Pro  Glu Thr Ala Asp Ile  Thr Glu Met Glu Glu  Pro Phe Val 
    1055                 1060                 1065             


Ser His  Met Pro Asn Arg Lys  Ile His Gly Pro Ala  His Lys Glu 
    1070                 1075                 1080             


Thr Ile  Arg Ser Gly Arg Leu  Lys Glu Glu Gly Tyr  Thr Ile Ser 
    1085                 1090                 1095             


Lys Thr  Ala Leu Ile Asp Leu  Lys Leu Thr Glu Asp  Lys Glu Glu 
    1100                 1105                 1110             


Ile Lys  Gly Tyr Tyr Asn Lys  Glu Ser Asp Arg Leu  Leu Tyr Glu 
    1115                 1120                 1125             


Ala Leu  Lys Lys Gln Leu Gln  Arg Tyr Gly Gly Lys  Ala Lys Glu 
    1130                 1135                 1140             


Ala Phe  Lys Glu Pro Phe His  Lys Pro Lys Ala Asp  Gly Thr Pro 
    1145                 1150                 1155             


Gly Pro  Ile Val Asn Lys Val  Lys Ile Met Glu Lys  Ser Thr Met 
    1160                 1165                 1170             


Leu Ile  Pro Val Asn Gly Gly  Lys Gly Leu Ala Ser  Asn Gly Asn 
    1175                 1180                 1185             


Met Val  Arg Ile Asp Val Phe  Arg Ala Glu Glu Lys  Gly Lys Lys 
    1190                 1195                 1200             


Lys Tyr  Tyr Phe Ile Pro Val  Tyr Val Ala Asp Thr  Val Lys Glu 
    1205                 1210                 1215             


Glu Leu  Pro Asn Arg Ala Val  Leu Ala His Lys Pro  Tyr Glu Ala 
    1220                 1225                 1230             


Trp Lys  Ile Met Lys Glu Glu  Asn Phe Ile Phe Ser  Leu Tyr Pro 
    1235                 1240                 1245             


Asn Asp  Leu Ile Phe Val Asp  Ala Gly Lys Glu Ile  Pro Phe Lys 
    1250                 1255                 1260             


Ala Ala  Leu Lys Gly Ser Thr  Leu Asp Pro Glu Lys  Lys Ala Ser 
    1265                 1270                 1275             


Arg Phe  Leu Met Tyr Tyr Lys  Gly Ala Asp Ile Ala  Thr Gly Ser 
    1280                 1285                 1290             


Ile Ser  Gly Val Asn His Asp  Glu Thr Tyr Lys Ala  Arg Gly Val 
    1295                 1300                 1305             


Gly Ile  Gln Ser Leu Arg Glu  Ile Lys Lys Cys Cys  Ile Asp Val 
    1310                 1315                 1320             


Leu Gly  Asn Ile Ser Phe Ala  Ser Lys Glu Lys Arg  Gln Thr Phe 
    1325                 1330                 1335             


Arg Ser  Gly Gly Ser Ser Ile  Val Arg Val Thr Tyr  Asn Val Glu 
    1340                 1345                 1350             


Gln Glu  Val Glu Ile Ser Asn  Lys Phe Asn Lys Asp  Val Asp Ile 
    1355                 1360                 1365             


Met Lys  Pro Glu Glu Tyr Asp  Asp Tyr Ile Asp Asp  Leu Met Phe 
    1370                 1375                 1380             


Ala Leu  Asn Glu Lys Leu Pro  Glu Tyr Ala Glu Ile  Ser Cys Val 
    1385                 1390                 1395             


Ile Asn  Asp Asn Thr Gly Glu  Val Leu Phe Glu Gly  Gly Ser Gly 
    1400                 1405                 1410             


Gly Ser  Pro Lys Lys Lys Arg  Lys Val 
    1415                 1420         


<210> 483
<211> 10943
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: Plasmid

<220>
<223> Description: pET21-CAT (H193Y)-sgRNA-TadA-nSpCas9 (D10A)

<400> 483
gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagtc atcatcatca       60

ccatcacatg tctgaagtcg aatttagcca cgaatactgg atgcgtcacg cgctgacgct      120

ggcgaaacgt gcctgggatg agcgggaagt gccggtcggc gcggtattag tgcataacaa      180

tcgggtaatc ggcgaaggct ggaaccgccc gattggtcgc catgatccca ccgcacatgc      240

agaaatcatg gccctgcggc agggtggtct ggtgatgcaa aattatcgtc tgatcgacgc      300

cacgttgtat gtcacgcttg aaccatgtgt aatgtgtgcc ggagcgatga tccacagtcg      360

cattggtcgc gtggtctttg gtgcgcgtga cgcgaaaact ggcgctgcgg gatctttaat      420

ggatgtgctg catcatccgg gtatgaatca ccgagtggaa attacggaag gaatactggc      480

ggatgagtgc gcggcgttgc tcagtgactt ctttcgcatg cgccgccagg aaattaaagc      540

gcagaaaaaa gcgcaatcct cgacggattc aggcgggtcg tcgggaggaa gcagtggcag      600

cgagactccc ggaacttcgg agagcgctac accagaaagt agtggaggca gctctggtgg      660

gtccatggat aagaaatact caataggact ggcgattggc acaaatagcg tcggatgggc      720

tgtgatcact gatgaatata aggttccttc taaaaagttc aaggttctgg gaaatacaga      780

ccgccacagt atcaaaaaaa atcttatagg ggctcttctg tttgacagtg gagagacagc      840

cgaagctact agactcaaac ggacagctag gagaaggtat acaagacgga agaataggat      900

ttgttatctc caggagattt tttcaaatga gatggccaaa gtggatgata gtttctttca      960

tagacttgaa gagtcttttt tggtggaaga agacaagaag catgaaagac atcctatttt     1020

tggaaatata gtggatgaag ttgcttatca cgagaaatat ccaactatct atcatctgag     1080

aaaaaaattg gtggattcta ctgataaagc cgatttgcgc ctgatctatt tggccctggc     1140

ccacatgatt aagtttagag gtcatttttt gattgagggc gatctgaatc ctgataatag     1200

tgatgtggac aaactgttta tccagttggt gcaaacctac aatcaactgt ttgaagaaaa     1260

ccctattaac gcaagtggag tggatgctaa agccattctt tctgcaagat tgagtaaatc     1320

aagaagactg gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcctgtttgg     1380

gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc     1440

agaagatgct aaactccagc tttcaaaaga tacttacgat gatgatctgg ataatctgtt     1500

ggctcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatc tgtcagatgc     1560

tattctgctt tcagacatcc tgagagtgaa tactgaaata actaaggctc ccctgtcagc     1620

ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttctga aagccctggt     1680

tagacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata     1740

tgcaggttat attgatggcg gcgcaagcca agaagaattt tataaattta tcaaaccaat     1800

tctggaaaaa atggatggta ctgaggaact gttggtgaaa ctgaatagag aagatttgct     1860

gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct     1920

gcatgctatt ttgagaagac aagaagactt ttatccattt ctgaaagaca atagagagaa     1980

gattgaaaaa atcttgactt ttaggattcc ttattatgtt ggtccattgg ccagaggcaa     2040

tagtaggttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga     2100

agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga     2160

taaaaatctt ccaaatgaaa aagtgctgcc aaaacatagt ttgctttatg agtattttac     2220

cgtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgagaa aaccagcatt     2280

tctttcaggt gaacagaaga aagccattgt tgatctgctc ttcaaaacaa ataggaaagt     2340

gaccgttaag caactgaaag aagattattt caaaaaaata gaatgttttg atagtgttga     2400

aatttcagga gttgaagata gatttaatgc ttcactgggt acataccatg atttgctgaa     2460

aattattaaa gataaagatt ttttggataa tgaagaaaat gaagacatcc tggaggatat     2520

tgttctgaca ttgaccctgt ttgaagatag ggagatgatt gaggaaagac ttaaaacata     2580

cgctcacctc tttgatgata aggtgatgaa acagcttaaa agacgcagat atactggttg     2640

gggaaggttg tccagaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat     2700

actggatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tcatccatga     2760

tgatagtttg acatttaaag aagacatcca aaaagcacaa gtgtctggac aaggcgatag     2820

tctgcatgaa catattgcaa atctggctgg tagccctgct attaaaaaag gtattctcca     2880

gactgtgaaa gttgttgatg aattggtcaa agtgatgggg cggcataagc cagaaaatat     2940

cgttattgaa atggcaagag aaaatcagac aactcaaaag ggccagaaaa attccagaga     3000

gaggatgaaa agaatcgaag aaggtatcaa agaactggga agtcagattc ttaaagagca     3060

tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg     3120

aagagatatg tatgtggacc aagaactgga tattaatagg ctgagtgatt atgatgtcga     3180

tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcctgaccag     3240

gtctgataaa aatagaggta aatccgataa cgttccaagt gaagaagtgg tcaaaaagat     3300

gaaaaactat tggagacaac ttctgaacgc caagctgatc actcaaagga agtttgataa     3360

tctgaccaaa gctgaaagag gaggtttgag tgaacttgat aaagctggtt ttatcaaacg     3420

ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat     3480

gaatactaaa tacgatgaaa atgataaact tattagagag gttaaagtga ttaccctgaa     3540

atctaaactg gtttctgact tcagaaaaga tttccaattc tataaagtga gagagattaa     3600

caattaccat catgcccatg atgcctatct gaatgccgtc gttggaactg ctttgattaa     3660

gaaatatcca aaacttgaaa gcgagtttgt ctatggtgat tataaagttt atgatgttag     3720

gaaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaagt atttctttta     3780

ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa     3840

acgccctctg atcgaaacta atggggaaac tggagaaatt gtctgggata aagggagaga     3900

ttttgccaca gtgcgcaaag tgttgtccat gccccaagtc aatatcgtca agaaaacaga     3960

agtgcagaca ggcggattct ctaaggagtc aattctgcca aaaagaaatt ccgacaagct     4020

gattgctagg aaaaaagact gggacccaaa aaaatatggt ggttttgata gtccaaccgt     4080

ggcttattca gtcctggtgg ttgctaaggt ggaaaaaggg aaatccaaga agctgaaatc     4140

cgttaaagag ctgctgggga tcacaattat ggaaagaagt tcctttgaaa aaaatcccat     4200

tgactttctg gaagctaaag gatataagga agttaaaaaa gacctgatca ttaaactgcc     4260

taaatatagt ctttttgagc tggaaaacgg taggaaacgg atgctggcta gtgccggaga     4320

actgcaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttc tgtatctggc     4380

tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt     4440

ggagcagcat aagcattatc tggatgagat tattgagcaa atcagtgaat tttctaagag     4500

agttattctg gcagatgcca atctggataa agttcttagt gcatataaca aacatagaga     4560

caaaccaata agagaacaag cagaaaatat cattcatctg tttaccttga ccaatcttgg     4620

agcacccgct gcttttaaat actttgatac aacaattgat aggaaaagat atacctctac     4680

aaaagaagtt ctggatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg     4740

cattgatttg agtcagctgg gaggtgacgg ctctggcggg tctcccaaga agaagaggaa     4800

agtctaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc     4860

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     4920

gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc     4980

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc     5040

gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct     5100

ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa     5160

aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc     5220

cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca     5280

ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat     5340

tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg     5400

cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     5460

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     5520

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt     5580

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg     5640

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga     5700

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc     5760

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac     5820

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg     5880

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca     5940

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg     6000

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg     6060

acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg     6120

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag     6180

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg     6240

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct     6300

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     6360

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     6420

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     6480

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     6540

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     6600

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     6660

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     6720

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     6780

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     6840

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     6900

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     6960

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     7020

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     7080

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     7140

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     7200

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     7260

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     7320

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg     7380

gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag     7440

ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca     7500

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     7560

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     7620

aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt     7680

tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag     7740

cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga     7800

tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt     7860

actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg     7920

atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat     7980

gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg     8040

cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat     8100

gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc     8160

ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac     8220

aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg     8280

ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg     8340

aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa     8400

atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata     8460

agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct     8520

ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg     8580

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc     8640

ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac     8700

cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa     8760

gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg     8820

gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac     8880

gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac     8940

cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga     9000

catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata     9060

tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag     9120

cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc     9180

atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg     9240

aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat     9300

gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac     9360

gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt     9420

aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat     9480

cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc     9540

cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac     9600

cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac     9660

tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg     9720

aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct     9780

gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg     9840

gtttaattaa tttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc     9900

actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga     9960

gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt    10020

tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac    10080

ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat    10140

tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct    10200

ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt    10260

ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca    10320

agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat    10380

gttttttgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa    10440

tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa    10500

ggtgctgatg ccgctggcga ttcaggttca ttatgccgtc tgtgatggct tccatgtcgg    10560

cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt    10620

ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgaagcatgc    10680

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa    10740

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata    10800

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag    10860

aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg    10920

ataacaattc ccctctagga tcc                                            10943


<210> 484
<211> 8520
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: Plasmid

<220>
<223> Description: pET21-sgRNA-TadA (ABE8.17m)-nMG34-1 (D10A)

<400> 484
gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagtc atcatcatca       60

ccatcactcc gaggttgaat ttagtcatga atattggatg cgccacgcgc tgacgttagc      120

caaacgtgct cgtgatgagc gcgaagttcc agtcggggcc gtcttggtcc ttaacaatcg      180

tgtcattggc gaggggtgga accgcgctat tgggctgcac gatccaacag cgcatgctga      240

gattatggcc ttgcgtcaag gagggttagt aatgcaaaac taccgtctta ttgatgctac      300

gttatactct acattcgaac cctgtgtaat gtgtgcagga gcgatgatcc acagtcgtat      360

tggccgcgta gtatttggtg tacgcaatgc taaaactgga gccgccggat cgttgatgga      420

tgtcttgcat taccctggaa tgaaccatcg tgtagagatc actgaaggca tccttgccga      480

tgagtgtgcg gctttgcttt gctatttctt ccgtatgcca cgtcgtgttt ttaacgccca      540

aaaaaaggct caatccagta ccgattcagg cgggtcgtcg ggaggaagca gtggcagcga      600

gactcccgga acttcggaga gcgctacacc agaaagtagt ggaggcagct ctggtgggtc      660

catggagcgc gagctggtgc tggggatcgc gtatggtggt aagtacacgg gcttggcggt      720

ggtggatcgc cgccacaacc aagtgctgta cgcgaatcgt cttaaaatgc gtgatgacgt      780

ggcaggcatc ttgaaggacc gtcgcaaaca gcgcgggatt cgtcgcacgg cccagacgaa      840

gaagaaacgc cttcgcgagc tgaagaatta cctgaagagc atcgggtaca atgagtcgac      900

ggccaccttc gagaccgtgt attcactggc gcacaagcgt ggttacgact acgcagacat      960

gccagaggag aaaaccagtg aggagattga agcgatggac gttgaggaac gtaagcaatg     1020

ggagaaggaa aagcaagagt gggaagaaac taagcgcaac tctcgccatc gcaaagaggt     1080

agttaaggac gtacacaagg cgatgatcga gggccgtgcc acggaagaac aaattaagcg     1140

tgtggagcgc atcttcaaca agcaatatcg cccgaagcgt tttaacaacc gcatcttgac     1200

caagtgcaaa gtggaagact gtggtgtgaa cactcctttg cgtaagaacg tacgcgacct     1260

gctgattgag aacattgttc gtttctttcc gattgagcag agcgagaaag acaacctgaa     1320

ggacgccgta ttagacaaga atcgtcgcga agaggtgaag tccttctttc gcaagcacaa     1380

aaccgacgag cacatccgta aacaagtgta cgacattgcc gacaataaac tgagcgggcg     1440

tactgtattc tgcaaggagc acattctgga gcacacggaa cactccaagg aagagcgtaa     1500

agtctttcgc ctggcaccga gtctgaagac gaagattgaa aacgtgctgg cagtaatcaa     1560

ggatgaaatt ctccctaagt tcacggtgaa caaagtagtg atggaatcaa ataacttcga     1620

catcgccgcg aagacccaag gtaagaagcg tctcgccaag gaagagtacg gcaagggccc     1680

gcgtgagggt aaggaaacgc gcaaagaggc gctgctgcgc gagaccgacg ggcgctgcat     1740

ttattgcggc aagagcatcg atatttcgaa cgcacacgac gatcacatct tccctcgtaa     1800

agcgggcgga ttgaacattt tcgcgaacct cgtggcctgc tgtgcggtgt gcaacgaaaa     1860

caagaagggc cgcacgcctc tggagtcggg catttcacca aagccagaga ttattgcatt     1920

tatgaagaac gacctgaaga agaaaatctt ggaagatgct cgcaacatta acaccgtcga     1980

cttcaacaag tatatgagcc acgcttcgat tgggtggcgt tacatgcgcg atcgccttcg     2040

cgaatcggca ggtaacaaga aactgccgat cgaacgccaa agtggtattt atacggccta     2100

ctttcgtcgc tggtggggtt tcaagaaaga gcgcggtaat actttgcacc acgctctgga     2160

cgcggtgatt ttggcctcgc gtaagggcta ctcagacgac ggcttagtag acatgacttt     2220

aaaacccaag tacaataagg gcggcgaatt cgatccggag aagcacttac cggagcctat     2280

cgagttcaag atggacaagg ggtcacgcgg tagcgccctg cacgaccgta atccgttgag     2340

ttacaagaag ggtatcatca ctcgtcgctt catggtcact gaaatcgaat gtggcaagga     2400

agatgacgtg atctctgaaa cctaccgcga gaagttgaag gaagcgttca agcgcttcga     2460

caccaagaag ggcaagtgcc tgaccgataa agaagccaaa gaggcggggt tctgtatcaa     2520

gaagaacgag ctggtgatgt cgctgaagtg ttcaatcaag gggactggcc cgggccaaat     2580

gattcgcatt aacaacaacg tgttcaaaac caacgtacac aacgtaggag ttgacgtcta     2640

cctggacgag aagggcaaga agaaggcgta cgaacgcaag aacccacgct tatcgaagca     2700

ttttattgag ccgccgccac aaccgaacgg ccgcgtctct ttcacactga agcgtcgcga     2760

catggtgact gtagagggag aggatgccat ttaccgcatt aagaagttgg gtacttcccc     2820

gaccatcgag gcggtggtgg ggagcgacgg taagacgcgc acagtatcgg caacgaaatt     2880

gaccaaggcc aatagcgccg agctcgaggg ctctggcggg tctcccaaga agaagaggaa     2940

agtctaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc     3000

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     3060

gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc     3120

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc     3180

gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct     3240

ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa     3300

aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc     3360

cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca     3420

ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat     3480

tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg     3540

cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     3600

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     3660

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt     3720

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg     3780

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga     3840

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc     3900

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac     3960

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg     4020

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca     4080

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg     4140

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg     4200

acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg     4260

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag     4320

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg     4380

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct     4440

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     4500

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     4560

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     4620

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     4680

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     4740

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     4800

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     4860

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     4920

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     4980

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     5040

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     5100

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     5160

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     5220

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     5280

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     5340

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     5400

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     5460

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg     5520

gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag     5580

ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca     5640

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     5700

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     5760

aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt     5820

tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag     5880

cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga     5940

tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt     6000

actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg     6060

atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat     6120

gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg     6180

cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat     6240

gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc     6300

ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac     6360

aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg     6420

ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg     6480

aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa     6540

atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata     6600

agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct     6660

ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg     6720

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc     6780

ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac     6840

cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa     6900

gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg     6960

gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac     7020

gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac     7080

cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga     7140

catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata     7200

tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag     7260

cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc     7320

atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg     7380

aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat     7440

gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac     7500

gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt     7560

aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat     7620

cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc     7680

cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac     7740

cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac     7800

tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg     7860

aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct     7920

gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg     7980

gtttaattaa ttacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca     8040

ttccgcctga ccttaatacg actcactata gtctttatgg cagggtgaaa cgcgttacag     8100

ttaagaaatt aattgtaaaa cgcctataca gtgaagggat atacgcttgg gtttgtccag     8160

cctgagcctc tatgccagaa atggcgcctt catcgtgggt taggacattt aattttaaaa     8220

aaaaaccccg cttcggcggg gttttttttt agcatgcaag gagatggcgc ccaacagtcc     8280

cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg     8340

gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt     8400

ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg     8460

aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctaggatcc     8520


<210> 485
<211> 8925
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: Plasmid

<220>
<223> Description: pET21-sgRNA-rAPOBEC1-nMG34-1 (D10A)-UGI (PBS1)

<400> 485
atgggcagca gtcatcatca tcaccatcac atgtcgtctg agaccgggcc tgttgccgtg       60

gaccctaccc ttcgccgccg tatcgaacca cacgagtttg aagtgttttt tgacccacgc      120

gaactgcgca aagagacttg tttgctttac gagattaact ggggaggccg tcactccatc      180

tggcgtcaca cttcacaaaa tactaataag cacgttgagg ttaatttcat tgagaaattt      240

actacagagc gctacttctg tccgaataca cgttgcagca tcacctggtt tctttcctgg      300

tccccgtgcg gtgaatgttc acgtgccatc accgaattct tatcgcgcta ccctcacgta      360

actttgttta tctatattgc ccgtttatat caccatgcag accctcgcaa tcgtcagggc      420

ctgcgcgacc ttatctcgag tggagtcacc atccaaatta tgactgaaca ggaatcgggg      480

tattgttggc gcaactttgt caattattct ccgagcaacg aggcacattg gccgcgttac      540

ccacacttat gggtgcgcct ttacgtgctg gaattgtatt gcatcattct tggtcttccc      600

ccatgtttga atatccttcg ccgcaaacag cctcagttga cgttttttac gattgctctt      660

cagtcctgtc actaccaacg tcttcccccg cacatcctgt gggcgaccgg tttgaaatcg      720

gggagtgaaa cacccggcac atccgaaagt gcgacacctg aatctgccat ggagcgcgag      780

ctggtgctgg ggatcgcgta tggtggtaag tacacgggct tggcggtggt ggatcgccgc      840

cacaaccaag tgctgtacgc gaatcgtctt aaaatgcgtg atgacgtggc aggcatcttg      900

aaggaccgtc gcaaacagcg cgggattcgt cgcacggccc agacgaagaa gaaacgcctt      960

cgcgagctga agaattacct gaagagcatc gggtacaatg agtcgacggc caccttcgag     1020

accgtgtatt cactggcgca caagcgtggt tacgactacg cagacatgcc agaggagaaa     1080

accagtgagg agattgaagc gatggacgtt gaggaacgta agcaatggga gaaggaaaag     1140

caagagtggg aagaaactaa gcgcaactct cgccatcgca aagaggtagt taaggacgta     1200

cacaaggcga tgatcgaggg ccgtgccacg gaagaacaaa ttaagcgtgt ggagcgcatc     1260

ttcaacaagc aatatcgccc gaagcgtttt aacaaccgca tcttgaccaa gtgcaaagtg     1320

gaagactgtg gtgtgaacac tcctttgcgt aagaacgtac gcgacctgct gattgagaac     1380

attgttcgtt tctttccgat tgagcagagc gagaaagaca acctgaagga cgccgtatta     1440

gacaagaatc gtcgcgaaga ggtgaagtcc ttctttcgca agcacaaaac cgacgagcac     1500

atccgtaaac aagtgtacga cattgccgac aataaactga gcgggcgtac tgtattctgc     1560

aaggagcaca ttctggagca cacggaacac tccaaggaag agcgtaaagt ctttcgcctg     1620

gcaccgagtc tgaagacgaa gattgaaaac gtgctggcag taatcaagga tgaaattctc     1680

cctaagttca cggtgaacaa agtagtgatg gaatcaaata acttcgacat cgccgcgaag     1740

acccaaggta agaagcgtct cgccaaggaa gagtacggca agggcccgcg tgagggtaag     1800

gaaacgcgca aagaggcgct gctgcgcgag accgacgggc gctgcattta ttgcggcaag     1860

agcatcgata tttcgaacgc acacgacgat cacatcttcc ctcgtaaagc gggcggattg     1920

aacattttcg cgaacctcgt ggcctgctgt gcggtgtgca acgaaaacaa gaagggccgc     1980

acgcctctgg agtcgggcat ttcaccaaag ccagagatta ttgcatttat gaagaacgac     2040

ctgaagaaga aaatcttgga agatgctcgc aacattaaca ccgtcgactt caacaagtat     2100

atgagccacg cttcgattgg gtggcgttac atgcgcgatc gccttcgcga atcggcaggt     2160

aacaagaaac tgccgatcga acgccaaagt ggtatttata cggcctactt tcgtcgctgg     2220

tggggtttca agaaagagcg cggtaatact ttgcaccacg ctctggacgc ggtgattttg     2280

gcctcgcgta agggctactc agacgacggc ttagtagaca tgactttaaa acccaagtac     2340

aataagggcg gcgaattcga tccggagaag cacttaccgg agcctatcga gttcaagatg     2400

gacaaggggt cacgcggtag cgccctgcac gaccgtaatc cgttgagtta caagaagggt     2460

atcatcactc gtcgcttcat ggtcactgaa atcgaatgtg gcaaggaaga tgacgtgatc     2520

tctgaaacct accgcgagaa gttgaaggaa gcgttcaagc gcttcgacac caagaagggc     2580

aagtgcctga ccgataaaga agccaaagag gcggggttct gtatcaagaa gaacgagctg     2640

gtgatgtcgc tgaagtgttc aatcaagggg actggcccgg gccaaatgat tcgcattaac     2700

aacaacgtgt tcaaaaccaa cgtacacaac gtaggagttg acgtctacct ggacgagaag     2760

ggcaagaaga aggcgtacga acgcaagaac ccacgcttat cgaagcattt tattgagccg     2820

ccgccacaac cgaacggccg cgtctctttc acactgaagc gtcgcgacat ggtgactgta     2880

gagggagagg atgccattta ccgcattaag aagttgggta cttccccgac catcgaggcg     2940

gtggtgggga gcgacggtaa gacgcgcaca gtatcggcaa cgaaattgac caaggccaat     3000

agcgccgagc tcgaggggtc tggtggttct accaatcttt cagatattat tgaaaaagag     3060

acaggaaagc agttggtcat tcaagagagt atcctgatgt taccggagga agttgaggag     3120

gtaatcggaa ataaacccga gagcgacatt ttagtccaca ctgcttatga cgagagtacg     3180

gatgagaatg tgatgctgct tacgtcagat gcgcccgagt ataagccgtg ggccctggtc     3240

attcaggaca gtaacggcga aaacaagatc aaaatgcttt ctggtggttc tcccaagaag     3300

aagaggaaag tctaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct     3360

gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt     3420

tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg     3480

cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc     3540

tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc     3600

gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg     3660

accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg     3720

tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg     3780

gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt     3840

cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa     3900

tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg     3960

tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat     4020

gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat     4080

tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt     4140

aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag     4200

cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa     4260

agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg     4320

ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct     4380

tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac     4440

tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca     4500

caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat     4560

accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact     4620

attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc     4680

ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga     4740

taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg     4800

taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg     4860

aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca     4920

agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta     4980

ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca     5040

ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg     5100

cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga     5160

tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa     5220

tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc     5280

tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg     5340

tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac     5400

ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct     5460

acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc     5520

ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg     5580

gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg     5640

ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct     5700

ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga     5760

taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg     5820

cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca     5880

tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca     5940

tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac     6000

acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca     6060

gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga     6120

aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt     6180

ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc     6240

tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt     6300

aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga     6360

tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg     6420

cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta     6480

atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca     6540

taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga     6600

ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct     6660

cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc     6720

tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct     6780

gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca     6840

agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct     6900

cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga     6960

cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt     7020

tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat     7080

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt     7140

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt     7200

cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt     7260

tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt     7320

aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc     7380

gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg     7440

ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga     7500

aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga     7560

gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc     7620

gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta     7680

ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat     7740

aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga     7800

tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag     7860

gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg     7920

cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca     7980

acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa     8040

ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc     8100

tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat     8160

aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc     8220

ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg     8280

cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc     8340

aaggaatggt ttaattaatt acggccagtc atgcataatc cgcacgcatc tggaataagg     8400

aagtgccatt ccgcctgacc ttaatacgac tcactatagc gcgccggaga aaaccgcctc     8460

ggttacagtt aagaaattaa ttgtaaaacg cctatacagt gaagggatat acgcttgggt     8520

ttgtccagcc tgagcctcta tgccagaaat ggcgccttca tcgtgggtta ggacatttaa     8580

ttttaaaaaa aaaccccgct tcggcggggt ttttttttag catgcaagga gatggcgccc     8640

aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc     8700

ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc     8760

gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg     8820

atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc     8880

taggatccga aataattttg tttaacttta agaaggagat atacc                     8925


<210> 486
<211> 9519
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: Plasmid

<220>
<223> Description: pET21-CAT (H193Y)-sgRNA-MG68-4 (D109N)-nMG34-1 
      (D10A)

<400> 486
ttaattaatt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac       60

taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga      120

aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg      180

aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg      240

cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc      300

ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg      360

tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt      420

catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag      480

atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt      540

tttttgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata      600

tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg      660

tgctgatgcc gctggcgatt caggttcatt atgccgtctg tgatggcttc catgtcggca      720

gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt      780

aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg aagcatgcaa      840

ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca      900

agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata      960

ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag     1020

gatcgactcg agtgcggccg caagcttgtc gacggagctc gaattcggat cctaatacga     1080

ctcactatag taatgaacct gaatcgccag gttacagtta agaaattaat tgtaaaacgc     1140

ctatacagtg aagggatata cgcttgggtt tgtccagcct gagcctctat gccagaaatg     1200

gcgccttcat cgtgggttag gacatttaat tttaaaaaaa aaccccgctt cggcggggtt     1260

tttttttagc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca     1320

tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg     1380

tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga     1440

tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg     1500

ggaattgtga gcggataaca attcccctct aggatccgaa ataattttgt ttaactttaa     1560

gaaggagata taccccgcgg atgactgatt ctcaaaatga tacagatgaa tactggatgg     1620

gagaggccct gaagttggca caaaaggcat gggacgaagg agaggtgccc gtaggagcca     1680

tcttggttcg taagggcgaa gttatcggtc atggctggaa ccgtagcatc gggtcccatg     1740

acccctcggc gcatgctgaa attatggcta ttcgtgaagc cggggtgcgt atcggtaatt     1800

atcgcatgtt ggataccgta ttatacgtta cgctggagcc gtgtgcaatg tgcgcagggg     1860

cacttgttca cgggcgtatt tcacgcttgg tctttggtgc cgaaaacccc aagacagggg     1920

cgtgcgggtc agtcatggac attgctcgcc accctgcttt gaaccaccaa ttacaagttg     1980

atggtggggt aatggcggct ccttgtgcgc gtcttttatc tgacttcttt cgctggcgtc     2040

gcgctcagaa aaaggcggcc cgtgataata gttcggggaa cggtacctca ggcgggtcgt     2100

cgggaggaag cagtggcagc gagactcccg gaacttcgga gagcgctaca ccagaaagta     2160

gtggaggcag ctctggtggg tccatggagc gcgagctggt gctggggatc gcgtatggtg     2220

gtaagtacac gggcttggcg gtggtggatc gccgccacaa ccaagtgctg tacgcgaatc     2280

gtcttaaaat gcgtgatgac gtggcaggca tcttgaagga ccgtcgcaaa cagcgcggga     2340

ttcgtcgcac ggcccagacg aagaagaaac gccttcgcga gctgaagaat tacctgaaga     2400

gcatcgggta caatgagtcg acggccacct tcgagaccgt gtattcactg gcgcacaagc     2460

gtggttacga ctacgcagac atgccagagg agaaaaccag tgaggagatt gaagcgatgg     2520

acgttgagga acgtaagcaa tgggagaagg aaaagcaaga gtgggaagaa actaagcgca     2580

actctcgcca tcgcaaagag gtagttaagg acgtacacaa ggcgatgatc gagggccgtg     2640

ccacggaaga acaaattaag cgtgtggagc gcatcttcaa caagcaatat cgcccgaagc     2700

gttttaacaa ccgcatcttg accaagtgca aagtggaaga ctgtggtgtg aacactcctt     2760

tgcgtaagaa cgtacgcgac ctgctgattg agaacattgt tcgtttcttt ccgattgagc     2820

agagcgagaa agacaacctg aaggacgccg tattagacaa gaatcgtcgc gaagaggtga     2880

agtccttctt tcgcaagcac aaaaccgacg agcacatccg taaacaagtg tacgacattg     2940

ccgacaataa actgagcggg cgtactgtat tctgcaagga gcacattctg gagcacacgg     3000

aacactccaa ggaagagcgt aaagtctttc gcctggcacc gagtctgaag acgaagattg     3060

aaaacgtgct ggcagtaatc aaggatgaaa ttctccctaa gttcacggtg aacaaagtag     3120

tgatggaatc aaataacttc gacatcgccg cgaagaccca aggtaagaag cgtctcgcca     3180

aggaagagta cggcaagggc ccgcgtgagg gtaaggaaac gcgcaaagag gcgctgctgc     3240

gcgagaccga cgggcgctgc atttattgcg gcaagagcat cgatatttcg aacgcacacg     3300

acgatcacat cttccctcgt aaagcgggcg gattgaacat tttcgcgaac ctcgtggcct     3360

gctgtgcggt gtgcaacgaa aacaagaagg gccgcacgcc tctggagtcg ggcatttcac     3420

caaagccaga gattattgca tttatgaaga acgacctgaa gaagaaaatc ttggaagatg     3480

ctcgcaacat taacaccgtc gacttcaaca agtatatgag ccacgcttcg attgggtggc     3540

gttacatgcg cgatcgcctt cgcgaatcgg caggtaacaa gaaactgccg atcgaacgcc     3600

aaagtggtat ttatacggcc tactttcgtc gctggtgggg tttcaagaaa gagcgcggta     3660

atactttgca ccacgctctg gacgcggtga ttttggcctc gcgtaagggc tactcagacg     3720

acggcttagt agacatgact ttaaaaccca agtacaataa gggcggcgaa ttcgatccgg     3780

agaagcactt accggagcct atcgagttca agatggacaa ggggtcacgc ggtagcgccc     3840

tgcacgaccg taatccgttg agttacaaga agggtatcat cactcgtcgc ttcatggtca     3900

ctgaaatcga atgtggcaag gaagatgacg tgatctctga aacctaccgc gagaagttga     3960

aggaagcgtt caagcgcttc gacaccaaga agggcaagtg cctgaccgat aaagaagcca     4020

aagaggcggg gttctgtatc aagaagaacg agctggtgat gtcgctgaag tgttcaatca     4080

aggggactgg cccgggccaa atgattcgca ttaacaacaa cgtgttcaaa accaacgtac     4140

acaacgtagg agttgacgtc tacctggacg agaagggcaa gaagaaggcg tacgaacgca     4200

agaacccacg cttatcgaag cattttattg agccgccgcc acaaccgaac ggccgcgtct     4260

ctttcacact gaagcgtcgc gacatggtga ctgtagaggg agaggatgcc atttaccgca     4320

ttaagaagtt gggtacttcc ccgaccatcg aggcggtggt ggggagcgac ggtaagacgc     4380

gcacagtatc ggcaacgaaa ttgaccaagg ccaatagcgc cgagctcgag ggctctggcg     4440

ggtctcccaa gaagaagagg aaagtctaag atccggctgc taacaaagcc cgaaaggaag     4500

ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac     4560

gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc     4620

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac     4680

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt     4740

cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc     4800

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc     4860

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact     4920

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg     4980

gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc     5040

gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc     5100

ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa     5160

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc     5220

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa     5280

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa     5340

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg     5400

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa     5460

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc     5520

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc     5580

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta     5640

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag     5700

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca     5760

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata     5820

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     5880

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     5940

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     6000

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     6060

taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa     6120

tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt     6180

gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat     6240

cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg     6300

gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga     6360

gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac     6420

tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt     6480

ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag     6540

cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc     6600

gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag     6660

gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca     6720

gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt     6780

cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc     6840

tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc     6900

cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc     6960

cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat     7020

tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct     7080

gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat     7140

ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc     7200

ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc     7260

accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag     7320

cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt     7380

taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac     7440

tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga     7500

gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga     7560

gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg     7620

ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat     7680

gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac     7740

acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc     7800

gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca     7860

gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc     7920

ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg     7980

agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca     8040

gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg     8100

catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa     8160

ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt     8220

gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc     8280

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg     8340

ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct     8400

ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct     8460

gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca     8520

ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca     8580

gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt     8640

gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct     8700

gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag     8760

aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca     8820

cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag     8880

agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct     8940

ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca     9000

ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac     9060

ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca     9120

gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc     9180

ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag     9240

aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact     9300

ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg     9360

ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga     9420

cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg     9480

agcaccgccg ccgcaaggaa tggtttaatt aattctaga                            9519


<210> 487
<211> 11211
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: Plasmid

<220>
<223> Description: pET21-CAT (H193Y)-sgRNA-MG68-4 (D109N)-nSpCas9 
      (D10A)

<400> 487
gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagtc atcatcatca       60

ccatcacatg actgattctc aaaatgatac agatgaatac tggatgggag aggccctgaa      120

gttggcacaa aaggcatggg acgaaggaga ggtgcccgta ggagccatct tggttcgtaa      180

gggcgaagtt atcggtcatg gctggaaccg tagcatcggg tcccatgacc cctcggcgca      240

tgctgaaatt atggctattc gtgaagccgg ggtgcgtatc ggtaattatc gcatgttgga      300

taccgtatta tacgttacgc tggagccgtg tgcaatgtgc gcaggggcac ttgttcacgg      360

gcgtatttca cgcttggtct ttggtgccga aaaccccaag acaggggcgt gcgggtcagt      420

catggacatt gctcgccacc ctgctttgaa ccaccaatta caagttgatg gtggggtaat      480

ggcggctcct tgtgcgcgtc ttttatctga cttctttcgc tggcgtcgcg ctcagaaaaa      540

ggcggcccgt gataatagtt cggggaactc aggcgggtcg tcgggaggaa gcagtggcag      600

cgagactccc ggaacttcgg agagcgctac accagaaagt agtggaggca gctctggtgg      660

gtccatggat aagaaatact caataggact ggcgattggc acaaatagcg tcggatgggc      720

tgtgatcact gatgaatata aggttccttc taaaaagttc aaggttctgg gaaatacaga      780

ccgccacagt atcaaaaaaa atcttatagg ggctcttctg tttgacagtg gagagacagc      840

cgaagctact agactcaaac ggacagctag gagaaggtat acaagacgga agaataggat      900

ttgttatctc caggagattt tttcaaatga gatggccaaa gtggatgata gtttctttca      960

tagacttgaa gagtcttttt tggtggaaga agacaagaag catgaaagac atcctatttt     1020

tggaaatata gtggatgaag ttgcttatca cgagaaatat ccaactatct atcatctgag     1080

aaaaaaattg gtggattcta ctgataaagc cgatttgcgc ctgatctatt tggccctggc     1140

ccacatgatt aagtttagag gtcatttttt gattgagggc gatctgaatc ctgataatag     1200

tgatgtggac aaactgttta tccagttggt gcaaacctac aatcaactgt ttgaagaaaa     1260

ccctattaac gcaagtggag tggatgctaa agccattctt tctgcaagat tgagtaaatc     1320

aagaagactg gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcctgtttgg     1380

gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc     1440

agaagatgct aaactccagc tttcaaaaga tacttacgat gatgatctgg ataatctgtt     1500

ggctcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatc tgtcagatgc     1560

tattctgctt tcagacatcc tgagagtgaa tactgaaata actaaggctc ccctgtcagc     1620

ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttctga aagccctggt     1680

tagacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata     1740

tgcaggttat attgatggcg gcgcaagcca agaagaattt tataaattta tcaaaccaat     1800

tctggaaaaa atggatggta ctgaggaact gttggtgaaa ctgaatagag aagatttgct     1860

gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct     1920

gcatgctatt ttgagaagac aagaagactt ttatccattt ctgaaagaca atagagagaa     1980

gattgaaaaa atcttgactt ttaggattcc ttattatgtt ggtccattgg ccagaggcaa     2040

tagtaggttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga     2100

agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga     2160

taaaaatctt ccaaatgaaa aagtgctgcc aaaacatagt ttgctttatg agtattttac     2220

cgtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgagaa aaccagcatt     2280

tctttcaggt gaacagaaga aagccattgt tgatctgctc ttcaaaacaa ataggaaagt     2340

gaccgttaag caactgaaag aagattattt caaaaaaata gaatgttttg atagtgttga     2400

aatttcagga gttgaagata gatttaatgc ttcactgggt acataccatg atttgctgaa     2460

aattattaaa gataaagatt ttttggataa tgaagaaaat gaagacatcc tggaggatat     2520

tgttctgaca ttgaccctgt ttgaagatag ggagatgatt gaggaaagac ttaaaacata     2580

cgctcacctc tttgatgata aggtgatgaa acagcttaaa agacgcagat atactggttg     2640

gggaaggttg tccagaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat     2700

actggatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tcatccatga     2760

tgatagtttg acatttaaag aagacatcca aaaagcacaa gtgtctggac aaggcgatag     2820

tctgcatgaa catattgcaa atctggctgg tagccctgct attaaaaaag gtattctcca     2880

gactgtgaaa gttgttgatg aattggtcaa agtgatgggg cggcataagc cagaaaatat     2940

cgttattgaa atggcaagag aaaatcagac aactcaaaag ggccagaaaa attccagaga     3000

gaggatgaaa agaatcgaag aaggtatcaa agaactggga agtcagattc ttaaagagca     3060

tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg     3120

aagagatatg tatgtggacc aagaactgga tattaatagg ctgagtgatt atgatgtcga     3180

tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcctgaccag     3240

gtctgataaa aatagaggta aatccgataa cgttccaagt gaagaagtgg tcaaaaagat     3300

gaaaaactat tggagacaac ttctgaacgc caagctgatc actcaaagga agtttgataa     3360

tctgaccaaa gctgaaagag gaggtttgag tgaacttgat aaagctggtt ttatcaaacg     3420

ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat     3480

gaatactaaa tacgatgaaa atgataaact tattagagag gttaaagtga ttaccctgaa     3540

atctaaactg gtttctgact tcagaaaaga tttccaattc tataaagtga gagagattaa     3600

caattaccat catgcccatg atgcctatct gaatgccgtc gttggaactg ctttgattaa     3660

gaaatatcca aaacttgaaa gcgagtttgt ctatggtgat tataaagttt atgatgttag     3720

gaaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaagt atttctttta     3780

ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa     3840

acgccctctg atcgaaacta atggggaaac tggagaaatt gtctgggata aagggagaga     3900

ttttgccaca gtgcgcaaag tgttgtccat gccccaagtc aatatcgtca agaaaacaga     3960

agtgcagaca ggcggattct ctaaggagtc aattctgcca aaaagaaatt ccgacaagct     4020

gattgctagg aaaaaagact gggacccaaa aaaatatggt ggttttgata gtccaaccgt     4080

ggcttattca gtcctggtgg ttgctaaggt ggaaaaaggg aaatccaaga agctgaaatc     4140

cgttaaagag ctgctgggga tcacaattat ggaaagaagt tcctttgaaa aaaatcccat     4200

tgactttctg gaagctaaag gatataagga agttaaaaaa gacctgatca ttaaactgcc     4260

taaatatagt ctttttgagc tggaaaacgg taggaaacgg atgctggcta gtgccggaga     4320

actgcaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttc tgtatctggc     4380

tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt     4440

ggagcagcat aagcattatc tggatgagat tattgagcaa atcagtgaat tttctaagag     4500

agttattctg gcagatgcca atctggataa agttcttagt gcatataaca aacatagaga     4560

caaaccaata agagaacaag cagaaaatat cattcatctg tttaccttga ccaatcttgg     4620

agcacccgct gcttttaaat actttgatac aacaattgat aggaaaagat atacctctac     4680

aaaagaagtt ctggatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg     4740

cattgatttg agtcagctgg gaggtgacgg ctctggcggg tctcccaaga agaagaggaa     4800

agtctaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc     4860

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     4920

gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc     4980

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc     5040

gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct     5100

ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa     5160

aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc     5220

cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca     5280

ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat     5340

tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg     5400

cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     5460

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     5520

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt     5580

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg     5640

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga     5700

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc     5760

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac     5820

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg     5880

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca     5940

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg     6000

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg     6060

acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg     6120

gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag     6180

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg     6240

gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct     6300

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     6360

agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact     6420

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     6480

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     6540

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     6600

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     6660

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     6720

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     6780

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     6840

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     6900

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     6960

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     7020

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     7080

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     7140

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     7200

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     7260

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     7320

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg     7380

gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag     7440

ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca     7500

acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     7560

gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     7620

aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt     7680

tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag     7740

cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga     7800

tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt     7860

actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg     7920

atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat     7980

gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg     8040

cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat     8100

gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc     8160

ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac     8220

aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg     8280

ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg     8340

aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa     8400

atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata     8460

agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct     8520

ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg     8580

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc     8640

ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac     8700

cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa     8760

gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg     8820

gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac     8880

gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac     8940

cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga     9000

catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata     9060

tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag     9120

cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc     9180

atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg     9240

aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat     9300

gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac     9360

gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt     9420

aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat     9480

cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc     9540

cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac     9600

cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac     9660

tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg     9720

aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct     9780

gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg     9840

gtttaattaa tttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc     9900

actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga     9960

gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt    10020

tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac    10080

ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat    10140

tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct    10200

ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt    10260

ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca    10320

agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat    10380

gttttttgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa    10440

tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa    10500

ggtgctgatg ccgctggcga ttcaggttca ttatgccgtc tgtgatggct tccatgtcgg    10560

cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt    10620

ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgaagcatgc    10680

aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa    10740

caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata    10800

taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag    10860

aggatcgact cgagtgcggc cgcaagcttg tcgacggagc tcgaattcgg atcctaatac    10920

gactcactat agtaatgaac ctgaatcgcc aggttttaga gctagaaata gcaagttaaa    10980

ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtgaac    11040

ctgcaggagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat    11100

ctgttgtttg tcggtgaacg ctctcctgag taggacaaat tcgatcccgc gaaattaata    11160

cgactcacta taggggaatt gtgagcggat aacaattccc ctctaggatc c             11211


<210> 488
<211> 96
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: sgRNA scaffold sequence

<220>
<223> Description: MG15-1

<400> 488
gttgtaattc cctagaaata ggttattaca ataaggtcca acaggagtgt tggtaccgta       60

aagctctaac ggcacccacg ggtgccgtta tctttt                                 96


<210> 489
<211> 123
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Category: sgRNA scaffold sequence

<220>
<223> Description: MG34-1

<400> 489
gttacagtta agaaattaat tgtaaaacgc ctatacagtg aagggatata cgcttgggtt       60

tgtccagcct gagcctctat gccagaaatg gcgccttcat cgtgggttag gacatttaat      120

ttt                                                                    123


<210> 490
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A) in E. coli

<400> 490
gatccgcgct ggctaccggc ga                                                22


<210> 491
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-UGI (PBS1) in E. coli

<400> 491
gatccgcgct ggctaccggc ga                                                22


<210> 492
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-1 in E. coli

<400> 492
gatccgcgct ggctaccggc ga                                                22


<210> 493
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-2 in E. coli

<400> 493
gatccgcgct ggctaccggc ga                                                22


<210> 494
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nMG15-1 (D8A)-MG69-3 in E. coli

<400> 494
gatccgcgct ggctaccggc ga                                                22


<210> 495
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nSpCas9 (D10A)-UGI (PBS1) in HEK293T

<400> 495
ggcccagact gagcacgtga                                                   20


<210> 496
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nSpCas9 (D10A) in HEK293T

<400> 496
ggcccagact gagcacgtga                                                   20


<210> 497
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nSpCas9 (D10A)-MG69-1 in HEK293T

<400> 497
ggcccagact gagcacgtga                                                   20


<210> 498
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: rAPOBEC1-nSpCas9 (D10A)-MG69-2 in HEK293T

<400> 498
ggcccagact gagcacgtga                                                   20


<210> 499
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG1-4 (D9A)-MG69-1_site 1 in HEK293T

<400> 499
gattcccacc tctcaatacg tt                                                22


<210> 500
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG1-4 (D9A)-MG69-1_site 2 in HEK293T

<400> 500
cccaggcagg caggctctcc ga                                                22


<210> 501
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG1-4 (D9A)-MG69-1_site 3 in HEK293T

<400> 501
caccccctct ccctccatca gt                                                22


<210> 502
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG1-4 (D9A)-MG69-1_site 4 in HEK293T

<400> 502
tgtacacagt ttcccaagct ac                                                22


<210> 503
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 1 in HEK293T

<400> 503
ctggactctg gccactccct gg                                                22


<210> 504
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 2 in HEK293T

<400> 504
catccagctc cagccccaga gc                                                22


<210> 505
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 3 in HEK293T

<400> 505
gattccggct ctgagggcca gt                                                22


<210> 506
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 4 in HEK293T

<400> 506
gcccttctgt ttgtcccact tg                                                22


<210> 507
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 5 in HEK293T

<400> 507
ttggccaagc caggcctaga at                                                22


<210> 508
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 6 in HEK293T

<400> 508
ctcagctggt acacggcagg gt                                                22


<210> 509
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG3-6 (D13A)-MG69-1_site 7 in HEK293T

<400> 509
tcgcctatgg gaactctgga gg                                                22


<210> 510
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG4-2 (D28A)-MG69-1_site 1 in HEK293T

<400> 510
cctaacatgg ataagtccag at                                                22


<210> 511
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG4-2 (D28A)-MG69-1_site 2 in HEK293T

<400> 511
ggccctcaga gccggaatct ac                                                22


<210> 512
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG4-2 (D28A)-MG69-1_site 3 in HEK293T

<400> 512
tgtcctccct caagatacag ga                                                22


<210> 513
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG4-2 (D28A)-MG69-1_site 4 in HEK293T

<400> 513
tgtgttcacc tgcccttctg tt                                                22


<210> 514
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG18-1 (D12A)-MG69-1_site 1 in HEK293T

<400> 514
cagtgtcccc cttccctatg gg                                                22


<210> 515
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG18-1 (D12A)-MG69-1_site 2 in HEK293T

<400> 515
cccccttccc tatgggaata at                                                22


<210> 516
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG18-1 (D12A)-MG69-1_site 3 in HEK293T

<400> 516
cagctccagc cccagagcct gg                                                22


<210> 517
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nMG18-1 (D12A)-MG69-1_site 4 in HEK293T

<400> 517
gtgcctctcc ctgcagacgg ta                                                22


<210> 518
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nSpCas9 (D10A)-MG69-1_site 1 in HEK293T

<400> 518
ttgccacgaa gcaggccaat                                                   20


<210> 519
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nSpCas9 (D10A)-MG69-1_site 2 in HEK293T

<400> 519
cccggggccg ccattgacag                                                   20


<210> 520
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nSpCas9 (D10A)-MG69-1_site 3 in HEK293T

<400> 520
tccggctctg agggccagtg                                                   20


<210> 521
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nSpCas9 (D10A)-MG69-1_site 4 in HEK293T

<400> 521
caaccagtcc ccagtgactc                                                   20


<210> 522
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Category: spacer

<220>
<223> Description: A0A2K5RDN7-nSpCas9 (D10A)-MG69-1_site 5 in HEK293T

<400> 522
gagtccgagc agaagaagaa                                                   20


<210> 523
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify lacZ of E. coli 
      and Sanger sequencing

<400> 523
ccaggcttta cactttatgc t                                                 21


<210> 524
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify lacZ of E. coli 
      and Sanger sequencing

<400> 524
cgaacatcca aaagtttgtg ttttt                                             25


<210> 525
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 525
ccgaaaggcg cggtgccg                                                     18


<210> 526
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 526
gtatgtggtg gatgaagcc                                                    19


<210> 527
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 527
ttgtggagcg acatccag                                                     18


<210> 528
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 528
tgagcgcatt tttacgcgc                                                    19


<210> 529
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 529
gaaaacggca acccgtgg                                                     18


<210> 530
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 530
ggattgaaaa tggtctgctg                                                   20


<210> 531
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing of base edit of lacZ of E. coli

<400> 531
actgctgacg ccgctgcg                                                     18


<210> 532
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify CAT (H193Y) of CAT 
      (H193Y)-sgRNA- MG68-4 variant-nSpCas9 (D10A)

<400> 532
ccgccgccgc aaggaatggt ttaattaatt tgatcggcac gtaagagg                    48


<210> 533
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify CAT (H193Y) of CAT 
      (H193Y)-sgRNA-MG68-4 variant-nSpCas9

<400> 533
ggactgttgg gcgccatctc cttgcatgct tcacttattc aggcgtagca                  50


<210> 534
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify CAT (H193Y) of CAT 
      (H193Y)-sgRNA-MG68-4 variant-nMG34-1 (D10A)

<400> 534
aaggaatggt ttaattaatt ctagattaat taatttgatc ggcacgtaag                  50


<210> 535
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Sanger sequencing primer of CAT (H193Y)

<400> 535
ggactgttgg gcgccatctc cttgcatgct tcacttattc aggcgtagca                  50


<210> 536
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify BE3 target site in 
      HEK293T cells and Sanger sequencing

<400> 536
gaggctggag aggcccgt                                                     18


<210> 537
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify BE3 target site in 
      HEK293T cells for Sanger sequencing

<400> 537
gattttcatg caggtgctga aa                                                22


<210> 538
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 538
gctcttccga tctnnnnnag gaggaagggc ctgagt                                 36


<210> 539
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 539
gctcttccga tctnnnnntc tgccctcgtg ggtttg                                 36


<210> 540
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 540
gctcttccga tctnnnnnct ctggccactc cctggc                                 36


<210> 541
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 541
gctcttccga tctnnnnngg caggctctcc gaggag                                 36


<210> 542
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 542
gctcttccga tctnnnnngg gaataataaa agtctctctc ttaa                        44


<210> 543
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 543
gctcttccga tctnnnnncc ccctccacca gtaccc                                 36


<210> 544
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 544
gctcttccga tctnnnnncc tgtccttgga gaaccg                                 36


<210> 545
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 545
gctcttccga tctnnnnngc aggtgaacac aagagct                                37


<210> 546
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 5 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 546
gctcttccga tctnnnnnga aggtgtggtt ccagaac                                37


<210> 547
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nSpCas9 
      (D10A)-MG69-1_site 5 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 547
gctcttccga tctnnnnntc gatgtcctcc ccattg                                 36


<210> 548
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 548
gctcttccga tctnnnnnaa acaggctaga cataggga                               38


<210> 549
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 549
gctcttccga tctnnnnnga agccaccaga gtctcta                                37


<210> 550
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 550
gctcttccga tctnnnnngc cgccattgac agaggg                                 36


<210> 551
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 551
gctcttccga tctnnnnngc atcaaaacaa aagggagatt g                           41


<210> 552
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 552
gctcttccga tctnnnnncc tctgcccacc tcactt                                 36


<210> 553
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 553
gctcttccga tctnnnnngc catgtgggtt aatctgg                                37


<210> 554
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 554
gctcttccga tctnnnnncc ggacgcacct acccat                                 36


<210> 555
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG1-4 
      (D9A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 555
gctcttccga tctnnnnnct agatgggaat ggatggg                                37


<210> 556
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 556
gctcttccga tctnnnnnaa ccacaaaccc acgagg                                 36


<210> 557
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 557
gctcttccga tctnnnnntc aatggcggcc ccgggc                                 36


<210> 558
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 558
gctcttccga tctnnnnnag tgatccccag tgtccc                                 36


<210> 559
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 559
gctcttccga tctnnnnngc cctgaacgcg tttgct                                 36


<210> 560
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 560
gctcttccga tctnnnnntg ggaataataa aagtctctct ct                          42


<210> 561
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 561
gctcttccga tctnnnnncc cctccaccag tacccc                                 36


<210> 562
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 562
gctcttccga tctnnnnnca gggcctcctc agccca                                 36


<210> 563
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 563
gctcttccga tctnnnnngt ctggatgtcg taagggaa                               38


<210> 564
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 5 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 564
gctcttccga tctnnnnngg ggtgtaactc agaatgtttt                             40


<210> 565
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 5 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 565
gctcttccga tctnnnnngg gagtgagact cagaga                                 36


<210> 566
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 6 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 566
gctcttccga tctnnnnngc aaagagggaa atgagatca                              39


<210> 567
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 6 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 567
gctcttccga tctnnnnngt gacacatttg tttgagaatc a                           41


<210> 568
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 7 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 568
gctcttccga tctnnnnnct ttatccccgc acagag                                 36


<210> 569
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG3-6 
      (D13A)-MG69-1_site 7 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 569
gctcttccga tctnnnnnct tggcccatgg gaaatc                                 36


<210> 570
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 570
gctcttccga tctnnnnngt cccatcccaa cacccc                                 36


<210> 571
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 571
gctcttccga tctnnnnntg ggcatgtgtg ctccca                                 36


<210> 572
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 572
gctcttccga tctnnnnnct atgggaataa taaaagtctc tc                          42


<210> 573
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 573
gctcttccga tctnnnnnct ccaccagtac cccacc                                 36


<210> 574
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 574
gctcttccga tctnnnnngg accctggtct ctacct                                 36


<210> 575
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 575
gctcttccga tctnnnnncc tctcccattg aactacc                                37


<210> 576
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 576
gctcttccga tctnnnnncc ccagtgactc agggcc                                 36


<210> 577
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG4-2 
      (D28A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 577
gctcttccga tctnnnnntc gtaagggaaa gacttaggaa                             40


<210> 578
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 578
gctcttccga tctnnnnntc tcccttttgt tttgatgcat tt                          42


<210> 579
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 1 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 579
gctcttccga tctnnnnncc accccaggct ctgggg                                 36


<210> 580
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 580
gctcttccga tctnnnnncc ttttgttttg atgcatttct gttt                        44


<210> 581
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 2 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 581
gctcttccga tctnnnnnaa tctaccaccc caggct                                 36


<210> 582
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 582
gctcttccga tctnnnnnat ccccagtgtc cccctt                                 36


<210> 583
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 3 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 583
gctcttccga tctnnnnncc aggccctgaa cgcgtt                                 36


<210> 584
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Forward primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 584
gctcttccga tctnnnnnag gccaggcctg cggggg                                 36


<210> 585
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<220>
<223> Category: primer

<220>
<223> Description: Reverse primer used to amplify A0A2K5RDN7-nMG18-1 
      (D12A)-MG69-1_site 4 in HEK293T cells


<220>
<221> modified_base
<222> (14)..(18)
<223> a, c, t, g, unknown or other

<400> 585
gctcttccga tctnnnnncc aaaaactccc aaattagcaa a                           41


<210> 586
<211> 969
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: TadA (ABE8.17m)-nMG34-1 (D10A)

<400> 586
Met Gly Ser Ser His His His His His His Ser Glu Val Glu Phe Ser 
1               5                   10                  15      


His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg 
            20                  25                  30          


Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg 
        35                  40                  45              


Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr 
    50                  55                  60                  


Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln 
65                  70                  75                  80  


Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Ser Thr Phe Glu Pro Cys 
                85                  90                  95      


Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val 
            100                 105                 110         


Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp 
        115                 120                 125             


Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly 
    130                 135                 140                 


Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met 
145                 150                 155                 160 


Pro Arg Arg Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp 
                165                 170                 175     


Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
            180                 185                 190         


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
        195                 200                 205             


Met Glu Arg Glu Leu Val Leu Gly Ile Ala Tyr Gly Gly Lys Tyr Thr 
    210                 215                 220                 


Gly Leu Ala Val Val Asp Arg Arg His Asn Gln Val Leu Tyr Ala Asn 
225                 230                 235                 240 


Arg Leu Lys Met Arg Asp Asp Val Ala Gly Ile Leu Lys Asp Arg Arg 
                245                 250                 255     


Lys Gln Arg Gly Ile Arg Arg Thr Ala Gln Thr Lys Lys Lys Arg Leu 
            260                 265                 270         


Arg Glu Leu Lys Asn Tyr Leu Lys Ser Ile Gly Tyr Asn Glu Ser Thr 
        275                 280                 285             


Ala Thr Phe Glu Thr Val Tyr Ser Leu Ala His Lys Arg Gly Tyr Asp 
    290                 295                 300                 


Tyr Ala Asp Met Pro Glu Glu Lys Thr Ser Glu Glu Ile Glu Ala Met 
305                 310                 315                 320 


Asp Val Glu Glu Arg Lys Gln Trp Glu Lys Glu Lys Gln Glu Trp Glu 
                325                 330                 335     


Glu Thr Lys Arg Asn Ser Arg His Arg Lys Glu Val Val Lys Asp Val 
            340                 345                 350         


His Lys Ala Met Ile Glu Gly Arg Ala Thr Glu Glu Gln Ile Lys Arg 
        355                 360                 365             


Val Glu Arg Ile Phe Asn Lys Gln Tyr Arg Pro Lys Arg Phe Asn Asn 
    370                 375                 380                 


Arg Ile Leu Thr Lys Cys Lys Val Glu Asp Cys Gly Val Asn Thr Pro 
385                 390                 395                 400 


Leu Arg Lys Asn Val Arg Asp Leu Leu Ile Glu Asn Ile Val Arg Phe 
                405                 410                 415     


Phe Pro Ile Glu Gln Ser Glu Lys Asp Asn Leu Lys Asp Ala Val Leu 
            420                 425                 430         


Asp Lys Asn Arg Arg Glu Glu Val Lys Ser Phe Phe Arg Lys His Lys 
        435                 440                 445             


Thr Asp Glu His Ile Arg Lys Gln Val Tyr Asp Ile Ala Asp Asn Lys 
    450                 455                 460                 


Leu Ser Gly Arg Thr Val Phe Cys Lys Glu His Ile Leu Glu His Thr 
465                 470                 475                 480 


Glu His Ser Lys Glu Glu Arg Lys Val Phe Arg Leu Ala Pro Ser Leu 
                485                 490                 495     


Lys Thr Lys Ile Glu Asn Val Leu Ala Val Ile Lys Asp Glu Ile Leu 
            500                 505                 510         


Pro Lys Phe Thr Val Asn Lys Val Val Met Glu Ser Asn Asn Phe Asp 
        515                 520                 525             


Ile Ala Ala Lys Thr Gln Gly Lys Lys Arg Leu Ala Lys Glu Glu Tyr 
    530                 535                 540                 


Gly Lys Gly Pro Arg Glu Gly Lys Glu Thr Arg Lys Glu Ala Leu Leu 
545                 550                 555                 560 


Arg Glu Thr Asp Gly Arg Cys Ile Tyr Cys Gly Lys Ser Ile Asp Ile 
                565                 570                 575     


Ser Asn Ala His Asp Asp His Ile Phe Pro Arg Lys Ala Gly Gly Leu 
            580                 585                 590         


Asn Ile Phe Ala Asn Leu Val Ala Cys Cys Ala Val Cys Asn Glu Asn 
        595                 600                 605             


Lys Lys Gly Arg Thr Pro Leu Glu Ser Gly Ile Ser Pro Lys Pro Glu 
    610                 615                 620                 


Ile Ile Ala Phe Met Lys Asn Asp Leu Lys Lys Lys Ile Leu Glu Asp 
625                 630                 635                 640 


Ala Arg Asn Ile Asn Thr Val Asp Phe Asn Lys Tyr Met Ser His Ala 
                645                 650                 655     


Ser Ile Gly Trp Arg Tyr Met Arg Asp Arg Leu Arg Glu Ser Ala Gly 
            660                 665                 670         


Asn Lys Lys Leu Pro Ile Glu Arg Gln Ser Gly Ile Tyr Thr Ala Tyr 
        675                 680                 685             


Phe Arg Arg Trp Trp Gly Phe Lys Lys Glu Arg Gly Asn Thr Leu His 
    690                 695                 700                 


His Ala Leu Asp Ala Val Ile Leu Ala Ser Arg Lys Gly Tyr Ser Asp 
705                 710                 715                 720 


Asp Gly Leu Val Asp Met Thr Leu Lys Pro Lys Tyr Asn Lys Gly Gly 
                725                 730                 735     


Glu Phe Asp Pro Glu Lys His Leu Pro Glu Pro Ile Glu Phe Lys Met 
            740                 745                 750         


Asp Lys Gly Ser Arg Gly Ser Ala Leu His Asp Arg Asn Pro Leu Ser 
        755                 760                 765             


Tyr Lys Lys Gly Ile Ile Thr Arg Arg Phe Met Val Thr Glu Ile Glu 
    770                 775                 780                 


Cys Gly Lys Glu Asp Asp Val Ile Ser Glu Thr Tyr Arg Glu Lys Leu 
785                 790                 795                 800 


Lys Glu Ala Phe Lys Arg Phe Asp Thr Lys Lys Gly Lys Cys Leu Thr 
                805                 810                 815     


Asp Lys Glu Ala Lys Glu Ala Gly Phe Cys Ile Lys Lys Asn Glu Leu 
            820                 825                 830         


Val Met Ser Leu Lys Cys Ser Ile Lys Gly Thr Gly Pro Gly Gln Met 
        835                 840                 845             


Ile Arg Ile Asn Asn Asn Val Phe Lys Thr Asn Val His Asn Val Gly 
    850                 855                 860                 


Val Asp Val Tyr Leu Asp Glu Lys Gly Lys Lys Lys Ala Tyr Glu Arg 
865                 870                 875                 880 


Lys Asn Pro Arg Leu Ser Lys His Phe Ile Glu Pro Pro Pro Gln Pro 
                885                 890                 895     


Asn Gly Arg Val Ser Phe Thr Leu Lys Arg Arg Asp Met Val Thr Val 
            900                 905                 910         


Glu Gly Glu Asp Ala Ile Tyr Arg Ile Lys Lys Leu Gly Thr Ser Pro 
        915                 920                 925             


Thr Ile Glu Ala Val Val Gly Ser Asp Gly Lys Thr Arg Thr Val Ser 
    930                 935                 940                 


Ala Thr Lys Leu Thr Lys Ala Asn Ser Ala Glu Leu Glu Gly Ser Gly 
945                 950                 955                 960 


Gly Ser Pro Lys Lys Lys Arg Lys Val 
                965                 


<210> 587
<211> 1104
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: cytosine base editor

<220>
<223> Description: rAPOBEC1-nMG34-1 (D10A)-UGI (PBS1)

<400> 587
Met Gly Ser Ser His His His His His His Met Ser Ser Glu Thr Gly 
1               5                   10                  15      


Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu 
            20                  25                  30          


Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu 
        35                  40                  45              


Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr 
    50                  55                  60                  


Ser Gln Asn Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe 
65                  70                  75                  80  


Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp 
                85                  90                  95      


Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu 
            100                 105                 110         


Phe Leu Ser Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg 
        115                 120                 125             


Leu Tyr His His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu 
    130                 135                 140                 


Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly 
145                 150                 155                 160 


Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His 
                165                 170                 175     


Trp Pro Arg Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu 
            180                 185                 190         


Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg 
        195                 200                 205             


Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His 
    210                 215                 220                 


Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Ser 
225                 230                 235                 240 


Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ala 
                245                 250                 255     


Met Glu Arg Glu Leu Val Leu Gly Ile Ala Tyr Gly Gly Lys Tyr Thr 
            260                 265                 270         


Gly Leu Ala Val Val Asp Arg Arg His Asn Gln Val Leu Tyr Ala Asn 
        275                 280                 285             


Arg Leu Lys Met Arg Asp Asp Val Ala Gly Ile Leu Lys Asp Arg Arg 
    290                 295                 300                 


Lys Gln Arg Gly Ile Arg Arg Thr Ala Gln Thr Lys Lys Lys Arg Leu 
305                 310                 315                 320 


Arg Glu Leu Lys Asn Tyr Leu Lys Ser Ile Gly Tyr Asn Glu Ser Thr 
                325                 330                 335     


Ala Thr Phe Glu Thr Val Tyr Ser Leu Ala His Lys Arg Gly Tyr Asp 
            340                 345                 350         


Tyr Ala Asp Met Pro Glu Glu Lys Thr Ser Glu Glu Ile Glu Ala Met 
        355                 360                 365             


Asp Val Glu Glu Arg Lys Gln Trp Glu Lys Glu Lys Gln Glu Trp Glu 
    370                 375                 380                 


Glu Thr Lys Arg Asn Ser Arg His Arg Lys Glu Val Val Lys Asp Val 
385                 390                 395                 400 


His Lys Ala Met Ile Glu Gly Arg Ala Thr Glu Glu Gln Ile Lys Arg 
                405                 410                 415     


Val Glu Arg Ile Phe Asn Lys Gln Tyr Arg Pro Lys Arg Phe Asn Asn 
            420                 425                 430         


Arg Ile Leu Thr Lys Cys Lys Val Glu Asp Cys Gly Val Asn Thr Pro 
        435                 440                 445             


Leu Arg Lys Asn Val Arg Asp Leu Leu Ile Glu Asn Ile Val Arg Phe 
    450                 455                 460                 


Phe Pro Ile Glu Gln Ser Glu Lys Asp Asn Leu Lys Asp Ala Val Leu 
465                 470                 475                 480 


Asp Lys Asn Arg Arg Glu Glu Val Lys Ser Phe Phe Arg Lys His Lys 
                485                 490                 495     


Thr Asp Glu His Ile Arg Lys Gln Val Tyr Asp Ile Ala Asp Asn Lys 
            500                 505                 510         


Leu Ser Gly Arg Thr Val Phe Cys Lys Glu His Ile Leu Glu His Thr 
        515                 520                 525             


Glu His Ser Lys Glu Glu Arg Lys Val Phe Arg Leu Ala Pro Ser Leu 
    530                 535                 540                 


Lys Thr Lys Ile Glu Asn Val Leu Ala Val Ile Lys Asp Glu Ile Leu 
545                 550                 555                 560 


Pro Lys Phe Thr Val Asn Lys Val Val Met Glu Ser Asn Asn Phe Asp 
                565                 570                 575     


Ile Ala Ala Lys Thr Gln Gly Lys Lys Arg Leu Ala Lys Glu Glu Tyr 
            580                 585                 590         


Gly Lys Gly Pro Arg Glu Gly Lys Glu Thr Arg Lys Glu Ala Leu Leu 
        595                 600                 605             


Arg Glu Thr Asp Gly Arg Cys Ile Tyr Cys Gly Lys Ser Ile Asp Ile 
    610                 615                 620                 


Ser Asn Ala His Asp Asp His Ile Phe Pro Arg Lys Ala Gly Gly Leu 
625                 630                 635                 640 


Asn Ile Phe Ala Asn Leu Val Ala Cys Cys Ala Val Cys Asn Glu Asn 
                645                 650                 655     


Lys Lys Gly Arg Thr Pro Leu Glu Ser Gly Ile Ser Pro Lys Pro Glu 
            660                 665                 670         


Ile Ile Ala Phe Met Lys Asn Asp Leu Lys Lys Lys Ile Leu Glu Asp 
        675                 680                 685             


Ala Arg Asn Ile Asn Thr Val Asp Phe Asn Lys Tyr Met Ser His Ala 
    690                 695                 700                 


Ser Ile Gly Trp Arg Tyr Met Arg Asp Arg Leu Arg Glu Ser Ala Gly 
705                 710                 715                 720 


Asn Lys Lys Leu Pro Ile Glu Arg Gln Ser Gly Ile Tyr Thr Ala Tyr 
                725                 730                 735     


Phe Arg Arg Trp Trp Gly Phe Lys Lys Glu Arg Gly Asn Thr Leu His 
            740                 745                 750         


His Ala Leu Asp Ala Val Ile Leu Ala Ser Arg Lys Gly Tyr Ser Asp 
        755                 760                 765             


Asp Gly Leu Val Asp Met Thr Leu Lys Pro Lys Tyr Asn Lys Gly Gly 
    770                 775                 780                 


Glu Phe Asp Pro Glu Lys His Leu Pro Glu Pro Ile Glu Phe Lys Met 
785                 790                 795                 800 


Asp Lys Gly Ser Arg Gly Ser Ala Leu His Asp Arg Asn Pro Leu Ser 
                805                 810                 815     


Tyr Lys Lys Gly Ile Ile Thr Arg Arg Phe Met Val Thr Glu Ile Glu 
            820                 825                 830         


Cys Gly Lys Glu Asp Asp Val Ile Ser Glu Thr Tyr Arg Glu Lys Leu 
        835                 840                 845             


Lys Glu Ala Phe Lys Arg Phe Asp Thr Lys Lys Gly Lys Cys Leu Thr 
    850                 855                 860                 


Asp Lys Glu Ala Lys Glu Ala Gly Phe Cys Ile Lys Lys Asn Glu Leu 
865                 870                 875                 880 


Val Met Ser Leu Lys Cys Ser Ile Lys Gly Thr Gly Pro Gly Gln Met 
                885                 890                 895     


Ile Arg Ile Asn Asn Asn Val Phe Lys Thr Asn Val His Asn Val Gly 
            900                 905                 910         


Val Asp Val Tyr Leu Asp Glu Lys Gly Lys Lys Lys Ala Tyr Glu Arg 
        915                 920                 925             


Lys Asn Pro Arg Leu Ser Lys His Phe Ile Glu Pro Pro Pro Gln Pro 
    930                 935                 940                 


Asn Gly Arg Val Ser Phe Thr Leu Lys Arg Arg Asp Met Val Thr Val 
945                 950                 955                 960 


Glu Gly Glu Asp Ala Ile Tyr Arg Ile Lys Lys Leu Gly Thr Ser Pro 
                965                 970                 975     


Thr Ile Glu Ala Val Val Gly Ser Asp Gly Lys Thr Arg Thr Val Ser 
            980                 985                 990         


Ala Thr Lys Leu Thr Lys Ala Asn  Ser Ala Glu Leu Glu  Gly Ser Gly 
        995                 1000                 1005             


Gly Ser  Thr Asn Leu Ser Asp  Ile Ile Glu Lys Glu  Thr Gly Lys 
    1010                 1015                 1020             


Gln Leu  Val Ile Gln Glu Ser  Ile Leu Met Leu Pro  Glu Glu Val 
    1025                 1030                 1035             


Glu Glu  Val Ile Gly Asn Lys  Pro Glu Ser Asp Ile  Leu Val His 
    1040                 1045                 1050             


Thr Ala  Tyr Asp Glu Ser Thr  Asp Glu Asn Val Met  Leu Leu Thr 
    1055                 1060                 1065             


Ser Asp  Ala Pro Glu Tyr Lys  Pro Trp Ala Leu Val  Ile Gln Asp 
    1070                 1075                 1080             


Ser Asn  Gly Glu Asn Lys Ile  Lys Met Leu Ser Gly  Gly Ser Pro 
    1085                 1090                 1095             


Lys Lys  Lys Arg Lys Val 
    1100                 


<210> 588
<211> 1592
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: MG68-3-nSpCas9 (D10A)

<400> 588
Met Gly Ser Ser His His His His His His Met Asn Glu Gln Thr Ser 
1               5                   10                  15      


Thr Asp Glu Gln Phe Met Gln Gln Ala Leu Ala Leu Ala Asp Glu Ala 
            20                  25                  30          


Glu Arg Gln Gly Glu Val Pro Val Gly Ala Leu Leu Val His Gln Gly 
        35                  40                  45              


Thr Val Ile Gly Arg Gly Phe Asn Gln Val Ile Thr Gln Leu Asp Pro 
    50                  55                  60                  


Ser Ala His Ala Glu Met Leu Ala Ile Arg Asp Ala Ala Arg Tyr Leu 
65                  70                  75                  80  


Gln Asn Tyr Arg Ile Leu Asp Thr Thr Leu Tyr Val Thr Leu Glu Pro 
                85                  90                  95      


Cys Pro Met Cys Ala Gly Ala Leu Val His Ala Arg Val Lys Arg Leu 
            100                 105                 110         


Val Tyr Gly Ala Ser Asp Pro Arg Thr Gly Ala Cys Gly Ser Val Phe 
        115                 120                 125             


Asn Val Val Ala Asp Thr Arg Leu Asn His Gln Leu Glu Val Val Ala 
    130                 135                 140                 


Gly Val Leu Gly Ser Gln Cys Gly Glu Arg Leu Ser Ala Phe Phe Arg 
145                 150                 155                 160 


Gln Arg Arg Ala Ala Gln Lys Ala Leu Lys Lys Ala Ala Gln Asn Phe 
                165                 170                 175     


Gln Ser Pro Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu 
            180                 185                 190         


Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser 
        195                 200                 205             


Ser Gly Gly Ser Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly 
    210                 215                 220                 


Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro 
225                 230                 235                 240 


Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys 
                245                 250                 255     


Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu 
            260                 265                 270         


Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys 
        275                 280                 285             


Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys 
    290                 295                 300                 


Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu 
305                 310                 315                 320 


Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp 
                325                 330                 335     


Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys 
            340                 345                 350         


Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu 
        355                 360                 365             


Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly 
    370                 375                 380                 


Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu 
385                 390                 395                 400 


Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser 
                405                 410                 415     


Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg 
            420                 425                 430         


Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly 
        435                 440                 445             


Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe 
    450                 455                 460                 


Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp 
                485                 490                 495     


Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile 
            500                 505                 510         


Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro 
        515                 520                 525             


Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu 
    530                 535                 540                 


Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys 
545                 550                 555                 560 


Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp 
                565                 570                 575     


Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu 
            580                 585                 590         


Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu 
        595                 600                 605             


Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His 
    610                 615                 620                 


Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp 
625                 630                 635                 640 


Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu 
                645                 650                 655     


Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser 
            660                 665                 670         


Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp 
        675                 680                 685             


Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile 
    690                 695                 700                 


Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu 
705                 710                 715                 720 


Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu 
                725                 730                 735     


Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu 
            740                 745                 750         


Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn 
        755                 760                 765             


Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile 
    770                 775                 780                 


Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn 
785                 790                 795                 800 


Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys 
                805                 810                 815     


Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val 
            820                 825                 830         


Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu 
        835                 840                 845             


Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys 
    850                 855                 860                 


Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn 
865                 870                 875                 880 


Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys 
                885                 890                 895     


Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp 
            900                 905                 910         


Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln 
        915                 920                 925             


Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala 
    930                 935                 940                 


Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val 
945                 950                 955                 960 


Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala 
                965                 970                 975     


Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg 
            980                 985                 990         


Met Lys Arg Ile Glu Glu Gly Ile  Lys Glu Leu Gly Ser  Gln Ile Leu 
        995                 1000                 1005             


Lys Glu  His Pro Val Glu Asn  Thr Gln Leu Gln Asn  Glu Lys Leu 
    1010                 1015                 1020             


Tyr Leu  Tyr Tyr Leu Gln Asn  Gly Arg Asp Met Tyr  Val Asp Gln 
    1025                 1030                 1035             


Glu Leu  Asp Ile Asn Arg Leu  Ser Asp Tyr Asp Val  Asp His Ile 
    1040                 1045                 1050             


Val Pro  Gln Ser Phe Leu Lys  Asp Asp Ser Ile Asp  Asn Lys Val 
    1055                 1060                 1065             


Leu Thr  Arg Ser Asp Lys Asn  Arg Gly Lys Ser Asp  Asn Val Pro 
    1070                 1075                 1080             


Ser Glu  Glu Val Val Lys Lys  Met Lys Asn Tyr Trp  Arg Gln Leu 
    1085                 1090                 1095             


Leu Asn  Ala Lys Leu Ile Thr  Gln Arg Lys Phe Asp  Asn Leu Thr 
    1100                 1105                 1110             


Lys Ala  Glu Arg Gly Gly Leu  Ser Glu Leu Asp Lys  Ala Gly Phe 
    1115                 1120                 1125             


Ile Lys  Arg Gln Leu Val Glu  Thr Arg Gln Ile Thr  Lys His Val 
    1130                 1135                 1140             


Ala Gln  Ile Leu Asp Ser Arg  Met Asn Thr Lys Tyr  Asp Glu Asn 
    1145                 1150                 1155             


Asp Lys  Leu Ile Arg Glu Val  Lys Val Ile Thr Leu  Lys Ser Lys 
    1160                 1165                 1170             


Leu Val  Ser Asp Phe Arg Lys  Asp Phe Gln Phe Tyr  Lys Val Arg 
    1175                 1180                 1185             


Glu Ile  Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
    1190                 1195                 1200             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1205                 1210                 1215             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1220                 1225                 1230             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1235                 1240                 1245             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1250                 1255                 1260             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1265                 1270                 1275             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1280                 1285                 1290             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1295                 1300                 1305             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1310                 1315                 1320             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1325                 1330                 1335             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1340                 1345                 1350             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1355                 1360                 1365             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1370                 1375                 1380             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1385                 1390                 1395             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1400                 1405                 1410             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1415                 1420                 1425             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1430                 1435                 1440             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1445                 1450                 1455             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1460                 1465                 1470             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1475                 1480                 1485             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1490                 1495                 1500             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1505                 1510                 1515             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1520                 1525                 1530             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1535                 1540                 1545             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1550                 1555                 1560             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1565                 1570                 1575             


Gly Asp  Gly Ser Gly Gly Ser  Pro Lys Lys Lys Arg  Lys Val 
    1580                 1585                 1590         


<210> 589
<211> 1588
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Category: adenine base editor

<220>
<223> Description: MG68-8-nSpCas9 (D10A)

<400> 589
Met Gly Ser Ser His His His His His His Met Pro Gln Ser Glu Gln 
1               5                   10                  15      


Asp Ile Tyr Trp Ile Arg Tyr Ala Met Gln Leu Ala Glu Arg Ala Glu 
            20                  25                  30          


Gln Ala Gly Glu Val Pro Val Gly Ala Val Leu Val Leu Asp Asn Gln 
        35                  40                  45              


Ala Leu Gly Glu Gly Trp Asn Leu Ser Ile Ser Arg His Asp Pro Cys 
    50                  55                  60                  


Ala His Ala Glu Ile Met Ala Ile Arg Gln Ala Gly Glu Arg Ile Gly 
65                  70                  75                  80  


Asn Tyr Arg Leu Leu Gly Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys 
                85                  90                  95      


Val Met Cys Ala Gly Ala Met Val His Ser Arg Ile Ala Arg Val Val 
            100                 105                 110         


Tyr Gly Ala Ser Asp Leu Lys Thr Gly Ala Ala Gly Ser Val Phe Asp 
        115                 120                 125             


Val Leu Ser Asp Pro Arg His Asn His Val Val Glu Ile Thr Gly Gly 
    130                 135                 140                 


Val Glu Ser Glu Ala Cys Ser Gln Gln Leu Ser Asp Phe Phe Arg Arg 
145                 150                 155                 160 


Arg Arg Ala Glu Gln Lys Ala Glu Lys Gln Arg Leu Lys Asp Asn Gln 
                165                 170                 175     


Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
            180                 185                 190         


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
        195                 200                 205             


Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
    210                 215                 220                 


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
225                 230                 235                 240 


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
                245                 250                 255     


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
            260                 265                 270         


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
        275                 280                 285             


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
    290                 295                 300                 


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
305                 310                 315                 320 


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
                325                 330                 335     


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
            340                 345                 350         


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
        355                 360                 365             


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
    370                 375                 380                 


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
385                 390                 395                 400 


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
                405                 410                 415     


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
            420                 425                 430         


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
        435                 440                 445             


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
    450                 455                 460                 


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
                485                 490                 495     


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
            500                 505                 510         


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
        515                 520                 525             


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
    530                 535                 540                 


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
545                 550                 555                 560 


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
                565                 570                 575     


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
            580                 585                 590         


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
        595                 600                 605             


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
    610                 615                 620                 


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
625                 630                 635                 640 


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
                645                 650                 655     


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
            660                 665                 670         


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
        675                 680                 685             


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
    690                 695                 700                 


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
705                 710                 715                 720 


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
                725                 730                 735     


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
            740                 745                 750         


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
        755                 760                 765             


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
    770                 775                 780                 


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
785                 790                 795                 800 


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
                805                 810                 815     


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
            820                 825                 830         


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
        835                 840                 845             


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
    850                 855                 860                 


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
865                 870                 875                 880 


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
                885                 890                 895     


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
            900                 905                 910         


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
        915                 920                 925             


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
    930                 935                 940                 


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
945                 950                 955                 960 


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
                965                 970                 975     


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
            980                 985                 990         


Glu Glu Gly Ile Lys Glu Leu Gly  Ser Gln Ile Leu Lys  Glu His Pro 
        995                 1000                 1005             


Val Glu  Asn Thr Gln Leu Gln  Asn Glu Lys Leu Tyr  Leu Tyr Tyr 
    1010                 1015                 1020             


Leu Gln  Asn Gly Arg Asp Met  Tyr Val Asp Gln Glu  Leu Asp Ile 
    1025                 1030                 1035             


Asn Arg  Leu Ser Asp Tyr Asp  Val Asp His Ile Val  Pro Gln Ser 
    1040                 1045                 1050             


Phe Leu  Lys Asp Asp Ser Ile  Asp Asn Lys Val Leu  Thr Arg Ser 
    1055                 1060                 1065             


Asp Lys  Asn Arg Gly Lys Ser  Asp Asn Val Pro Ser  Glu Glu Val 
    1070                 1075                 1080             


Val Lys  Lys Met Lys Asn Tyr  Trp Arg Gln Leu Leu  Asn Ala Lys 
    1085                 1090                 1095             


Leu Ile  Thr Gln Arg Lys Phe  Asp Asn Leu Thr Lys  Ala Glu Arg 
    1100                 1105                 1110             


Gly Gly  Leu Ser Glu Leu Asp  Lys Ala Gly Phe Ile  Lys Arg Gln 
    1115                 1120                 1125             


Leu Val  Glu Thr Arg Gln Ile  Thr Lys His Val Ala  Gln Ile Leu 
    1130                 1135                 1140             


Asp Ser  Arg Met Asn Thr Lys  Tyr Asp Glu Asn Asp  Lys Leu Ile 
    1145                 1150                 1155             


Arg Glu  Val Lys Val Ile Thr  Leu Lys Ser Lys Leu  Val Ser Asp 
    1160                 1165                 1170             


Phe Arg  Lys Asp Phe Gln Phe  Tyr Lys Val Arg Glu  Ile Asn Asn 
    1175                 1180                 1185             


Tyr His  His Ala His Asp Ala  Tyr Leu Asn Ala Val  Val Gly Thr 
    1190                 1195                 1200             


Ala Leu  Ile Lys Lys Tyr Pro  Lys Leu Glu Ser Glu  Phe Val Tyr 
    1205                 1210                 1215             


Gly Asp  Tyr Lys Val Tyr Asp  Val Arg Lys Met Ile  Ala Lys Ser 
    1220                 1225                 1230             


Glu Gln  Glu Ile Gly Lys Ala  Thr Ala Lys Tyr Phe  Phe Tyr Ser 
    1235                 1240                 1245             


Asn Ile  Met Asn Phe Phe Lys  Thr Glu Ile Thr Leu  Ala Asn Gly 
    1250                 1255                 1260             


Glu Ile  Arg Lys Arg Pro Leu  Ile Glu Thr Asn Gly  Glu Thr Gly 
    1265                 1270                 1275             


Glu Ile  Val Trp Asp Lys Gly  Arg Asp Phe Ala Thr  Val Arg Lys 
    1280                 1285                 1290             


Val Leu  Ser Met Pro Gln Val  Asn Ile Val Lys Lys  Thr Glu Val 
    1295                 1300                 1305             


Gln Thr  Gly Gly Phe Ser Lys  Glu Ser Ile Leu Pro  Lys Arg Asn 
    1310                 1315                 1320             


Ser Asp  Lys Leu Ile Ala Arg  Lys Lys Asp Trp Asp  Pro Lys Lys 
    1325                 1330                 1335             


Tyr Gly  Gly Phe Asp Ser Pro  Thr Val Ala Tyr Ser  Val Leu Val 
    1340                 1345                 1350             


Val Ala  Lys Val Glu Lys Gly  Lys Ser Lys Lys Leu  Lys Ser Val 
    1355                 1360                 1365             


Lys Glu  Leu Leu Gly Ile Thr  Ile Met Glu Arg Ser  Ser Phe Glu 
    1370                 1375                 1380             


Lys Asn  Pro Ile Asp Phe Leu  Glu Ala Lys Gly Tyr  Lys Glu Val 
    1385                 1390                 1395             


Lys Lys  Asp Leu Ile Ile Lys  Leu Pro Lys Tyr Ser  Leu Phe Glu 
    1400                 1405                 1410             


Leu Glu  Asn Gly Arg Lys Arg  Met Leu Ala Ser Ala  Gly Glu Leu 
    1415                 1420                 1425             


Gln Lys  Gly Asn Glu Leu Ala  Leu Pro Ser Lys Tyr  Val Asn Phe 
    1430                 1435                 1440             


Leu Tyr  Leu Ala Ser His Tyr  Glu Lys Leu Lys Gly  Ser Pro Glu 
    1445                 1450                 1455             


Asp Asn  Glu Gln Lys Gln Leu  Phe Val Glu Gln His  Lys His Tyr 
    1460                 1465                 1470             


Leu Asp  Glu Ile Ile Glu Gln  Ile Ser Glu Phe Ser  Lys Arg Val 
    1475                 1480                 1485             


Ile Leu  Ala Asp Ala Asn Leu  Asp Lys Val Leu Ser  Ala Tyr Asn 
    1490                 1495                 1500             


Lys His  Arg Asp Lys Pro Ile  Arg Glu Gln Ala Glu  Asn Ile Ile 
    1505                 1510                 1515             


His Leu  Phe Thr Leu Thr Asn  Leu Gly Ala Pro Ala  Ala Phe Lys 
    1520                 1525                 1530             


Tyr Phe  Asp Thr Thr Ile Asp  Arg Lys Arg Tyr Thr  Ser Thr Lys 
    1535                 1540                 1545             


Glu Val  Leu Asp Ala Thr Leu  Ile His Gln Ser Ile  Thr Gly Leu 
    1550                 1555                 1560             


Tyr Glu  Thr Arg Ile Asp Leu  Ser Gln Leu Gly Gly  Asp Gly Ser 
    1565                 1570                 1575             


Gly Gly  Ser Pro Lys Lys Lys  Arg Lys Val 
    1580                 1585             


<210> 590
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Category: Linker

<400> 590
Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


<210> 591
<211> 17
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Category: Linker

<400> 591
Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


Ala 
    


<210> 592
<211> 32
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Category: Linker

<400> 592
Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr 
1               5                   10                  15      


Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser 
            20                  25                  30          


<210> 593
<211> 34
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<220>
<223> Category: Linker

<400> 593
Gly Thr Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro 
1               5                   10                  15      


Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly 
            20                  25                  30          


Gly Ser 
        


