                         SEQUENCE LISTING

<110>  LIMMATECH BIOLOGICS AG
 
<120>  FAB SIALYATION OF ANTIBODIES

<130>  14197-013-228

<140>  TBA
<141>  

<150>  US 63/078,218
<151>  2020-09-14

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  357
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human UDP-GlcNAc Transporter (Uniprot Q9Y2D2), N-term 3xHA tag

<400>  1

Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Tyr Pro Tyr Asp Val Pro 
1               5                   10                  15      


Asp Tyr Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Gly Ser Gly 
            20                  25                  30          


Gly Phe Ala Asn Leu Lys Tyr Val Ser Leu Gly Ile Leu Val Phe Gln 
        35                  40                  45              


Thr Thr Ser Leu Val Leu Thr Met Arg Tyr Ser Arg Thr Leu Lys Glu 
    50                  55                  60                  


Glu Gly Pro Arg Tyr Leu Ser Ser Thr Ala Val Val Val Ala Glu Leu 
65                  70                  75                  80  


Leu Lys Ile Met Ala Cys Ile Leu Leu Val Tyr Lys Asp Ser Lys Cys 
                85                  90                  95      


Ser Leu Arg Ala Leu Asn Arg Val Leu His Asp Glu Ile Leu Asn Lys 
            100                 105                 110         


Pro Met Glu Thr Leu Lys Leu Ala Ile Pro Ser Gly Ile Tyr Thr Leu 
        115                 120                 125             


Gln Asn Asn Leu Leu Tyr Val Ala Leu Ser Asn Leu Asp Ala Ala Thr 
    130                 135                 140                 


Tyr Gln Val Thr Tyr Gln Leu Lys Ile Leu Thr Thr Ala Leu Phe Ser 
145                 150                 155                 160 


Val Ser Met Leu Ser Lys Lys Leu Gly Val Tyr Gln Trp Leu Ser Leu 
                165                 170                 175     


Val Ile Leu Met Thr Gly Val Ala Phe Val Gln Trp Pro Ser Asp Ser 
            180                 185                 190         


Gln Leu Asp Ser Lys Glu Leu Ser Ala Gly Ser Gln Phe Val Gly Leu 
        195                 200                 205             


Met Ala Val Leu Thr Ala Cys Phe Ser Ser Gly Phe Ala Gly Val Tyr 
    210                 215                 220                 


Phe Glu Lys Ile Leu Lys Glu Thr Lys Gln Ser Val Trp Ile Arg Asn 
225                 230                 235                 240 


Ile Gln Leu Gly Phe Phe Gly Ser Ile Phe Gly Leu Met Gly Val Tyr 
                245                 250                 255     


Ile Tyr Asp Gly Glu Leu Val Ser Lys Asn Gly Phe Phe Gln Gly Tyr 
            260                 265                 270         


Asn Arg Leu Thr Trp Ile Val Val Val Leu Gln Ala Leu Gly Gly Leu 
        275                 280                 285             


Val Ile Ala Ala Val Ile Lys Tyr Ala Asp Asn Ile Leu Lys Gly Phe 
    290                 295                 300                 


Ala Thr Ser Leu Ser Ile Ile Leu Ser Thr Leu Ile Ser Tyr Phe Trp 
305                 310                 315                 320 


Leu Gln Asp Phe Val Pro Thr Ser Val Phe Phe Leu Gly Ala Ile Leu 
                325                 330                 335     


Val Ile Thr Ala Thr Phe Leu Tyr Gly Tyr Asp Pro Lys Pro Ala Gly 
            340                 345                 350         


Asn Pro Thr Lys Ala 
        355         


<210>  2
<211>  344
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Beta-galactoside alpha-2,6-sialyltransferase 1 (Uniprot P15907), 
       aa89-406, N-terminal synthetic signal peptide

<400>  2

Met Ile Ala Ser Ser Val Arg His Ala Val Ile Leu Leu Leu Val Ala 
1               5                   10                  15      


Val Ala Met Met Val Asn Asn Val Ile Ala Pro Glu Ala Ser Phe Gln 
            20                  25                  30          


Val Trp Asn Lys Asp Ser Ser Ser Lys Asn Leu Ile Pro Arg Leu Gln 
        35                  40                  45              


Lys Ile Trp Lys Asn Tyr Leu Ser Met Asn Lys Tyr Lys Val Ser Tyr 
    50                  55                  60                  


Lys Gly Pro Gly Pro Gly Ile Lys Phe Ser Ala Glu Ala Leu Arg Cys 
65                  70                  75                  80  


His Leu Arg Asp His Val Asn Val Ser Met Val Glu Val Thr Asp Phe 
                85                  90                  95      


Pro Phe Asn Thr Ser Glu Trp Glu Gly Tyr Leu Pro Lys Glu Ser Ile 
            100                 105                 110         


Arg Thr Lys Ala Gly Pro Trp Gly Arg Cys Ala Val Val Ser Ser Ala 
        115                 120                 125             


Gly Ser Leu Lys Ser Ser Gln Leu Gly Arg Glu Ile Asp Asp His Asp 
    130                 135                 140                 


Ala Val Leu Arg Phe Asn Gly Ala Pro Thr Ala Asn Phe Gln Gln Asp 
145                 150                 155                 160 


Val Gly Thr Lys Thr Thr Ile Arg Leu Met Asn Ser Gln Leu Val Thr 
                165                 170                 175     


Thr Glu Lys Arg Phe Leu Lys Asp Ser Leu Tyr Asn Glu Gly Ile Leu 
            180                 185                 190         


Ile Val Trp Asp Pro Ser Val Tyr His Ser Asp Ile Pro Lys Trp Tyr 
        195                 200                 205             


Gln Asn Pro Asp Tyr Asn Phe Phe Asn Asn Tyr Lys Thr Tyr Arg Lys 
    210                 215                 220                 


Leu His Pro Asn Gln Pro Phe Tyr Ile Leu Lys Pro Gln Met Pro Trp 
225                 230                 235                 240 


Glu Leu Trp Asp Ile Leu Gln Glu Ile Ser Pro Glu Glu Ile Gln Pro 
                245                 250                 255     


Asn Pro Pro Ser Ser Gly Met Leu Gly Ile Ile Ile Met Met Thr Leu 
            260                 265                 270         


Cys Asp Gln Val Asp Ile Tyr Glu Phe Leu Pro Ser Lys Arg Lys Thr 
        275                 280                 285             


Asp Val Cys Tyr Tyr Tyr Gln Lys Phe Phe Asp Ser Ala Cys Thr Met 
    290                 295                 300                 


Gly Ala Tyr His Pro Leu Leu Tyr Glu Lys Asn Leu Val Lys His Leu 
305                 310                 315                 320 


Asn Gln Gly Thr Asp Glu Asp Ile Tyr Leu Leu Gly Lys Ala Thr Leu 
                325                 330                 335     


Pro Gly Phe Arg Thr Ile His Cys 
            340                 


<210>  3
<211>  579
<212>  PRT
<213>  Mouse

<220>
<223>  CMP-Neu5Ac transporter (Q61420.2), GFP fusion

<400>  3

Met Ala Pro Ala Arg Glu Asn Val Ser Leu Phe Phe Lys Leu Tyr Cys 
1               5                   10                  15      


Leu Thr Val Met Thr Leu Val Ala Ala Ala Tyr Thr Val Ala Leu Arg 
            20                  25                  30          


Tyr Thr Arg Thr Thr Ala Glu Glu Leu Tyr Phe Ser Thr Thr Ala Val 
        35                  40                  45              


Cys Ile Thr Glu Val Ile Lys Leu Leu Ile Ser Val Gly Leu Leu Ala 
    50                  55                  60                  


Lys Glu Thr Gly Ser Leu Gly Arg Phe Lys Ala Ser Leu Ser Glu Asn 
65                  70                  75                  80  


Val Leu Gly Ser Pro Lys Glu Leu Ala Lys Leu Ser Val Pro Ser Leu 
                85                  90                  95      


Val Tyr Ala Val Gln Asn Asn Met Ala Phe Leu Ala Leu Ser Asn Leu 
            100                 105                 110         


Asp Ala Ala Val Tyr Gln Val Thr Tyr Gln Leu Lys Ile Pro Cys Thr 
        115                 120                 125             


Ala Leu Cys Thr Val Leu Met Leu Asn Arg Thr Leu Ser Lys Leu Gln 
    130                 135                 140                 


Trp Ile Ser Val Phe Met Leu Cys Gly Gly Val Thr Leu Val Gln Trp 
145                 150                 155                 160 


Lys Pro Ala Gln Ala Thr Lys Val Val Val Ala Gln Asn Pro Leu Leu 
                165                 170                 175     


Gly Phe Gly Ala Ile Ala Ile Ala Val Leu Cys Ser Gly Phe Ala Gly 
            180                 185                 190         


Val Tyr Phe Glu Lys Val Leu Lys Ser Ser Asp Thr Ser Leu Trp Val 
        195                 200                 205             


Arg Asn Ile Gln Met Tyr Leu Ser Gly Ile Val Val Thr Leu Ala Gly 
    210                 215                 220                 


Thr Tyr Leu Ser Asp Gly Ala Glu Ile Gln Glu Lys Gly Phe Phe Tyr 
225                 230                 235                 240 


Gly Tyr Thr Tyr Tyr Val Trp Phe Val Ile Phe Leu Ala Ser Val Gly 
                245                 250                 255     


Gly Leu Tyr Thr Ser Val Val Val Lys Tyr Thr Asp Asn Ile Met Lys 
            260                 265                 270         


Gly Phe Ser Ala Ala Ala Ala Ile Val Leu Ser Thr Ile Ala Ser Val 
        275                 280                 285             


Leu Leu Phe Gly Leu Gln Ile Thr Leu Ser Phe Ala Leu Gly Ala Leu 
    290                 295                 300                 


Leu Val Cys Val Ser Ile Tyr Leu Tyr Gly Leu Pro Arg Gln Asp Thr 
305                 310                 315                 320 


Thr Ser Ile Gln Gln Glu Ala Thr Ser Lys Glu Arg Ile Ile Gly Val 
                325                 330                 335     


Gly Gly Ser Gly Gly Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val 
            340                 345                 350         


Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe 
        355                 360                 365             


Ser Val Arg Gly Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr 
    370                 375                 380                 


Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
385                 390                 395                 400 


Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro 
                405                 410                 415     


Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly 
            420                 425                 430         


Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys 
        435                 440                 445             


Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile 
    450                 455                 460                 


Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His 
465                 470                 475                 480 


Lys Leu Glu Tyr Asn Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp 
                485                 490                 495     


Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val 
            500                 505                 510         


Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
        515                 520                 525             


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr 
    530                 535                 540                 


Gln Ser Val Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
545                 550                 555                 560 


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu 
                565                 570                 575     


Leu Tyr Lys 
            


