                       SEQUENCE LISTING

<110>  Precirix N.V.

<120>  Antibody fragment against FAP

<130>  P6091373PCT

<150>  20195428.6
<151>  2020-09-10

<160>  55

<210>  1
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of B1

<400>  1
Gly Arg Leu Ser Ser Ser Asn Ser 
1               5               

<210>  2
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of B1

<400>  2
Ile Thr Gly Gly Gly Glu Thr 
1               5           

<210>  3
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of B1

<400>  3
Asn Phe Trp Pro Pro Leu Ile Asn Tyr 
1               5                   

<210>  4
<211>  115
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B1

<400>  4
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Leu Ser Ser 
                20                  25                  30  

Ser Asn Ser Met Ala Trp Tyr Arg Gln Val Pro Gly Lys Arg Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Gly Glu Thr Asn Tyr Ala 
                50                  55                  60  

Asp Phe Val Gly Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Gly Leu Tyr Leu Gln Leu Asn Gly Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Ala Tyr Tyr Cys Asn Phe Trp Pro Pro Leu Ile Asn Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115 

<210>  5
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of B2

<400>  5
Gly Ser Ile Ser Ser Ala Asn Ser 
1               5               

<210>  6
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of B2

<400>  6
Leu Thr Thr Gly Gly Arg Ser 
1               5           

<210>  7
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of B2

<400>  7
Asn Leu Trp Pro Pro Val Gln Gly Tyr 
1               5                   

<210>  8
<211>  115
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B2

<400>  8
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Ser Ile Ser Ser 
                20                  25                  30  

Ala Asn Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Asp Val Val Ala Gly Leu Thr Thr Gly Gly Arg Ser His Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Leu Trp Pro Pro Val Gln Gly Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115 

<210>  9
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of B3

<400>  9
Gly Arg Leu Phe Ser Thr Asn Ala 
1               5               

<210>  10
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of B3

<400>  10
Ile Thr Gly Gly Asp Arg Ser 
1               5           

<210>  11
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of B3

<400>  11
Asn Phe Tyr Pro Pro Ile Val Gly Asp Tyr 
1               5                   10  

<210>  12
<211>  116
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B3

<400>  12
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Leu Phe Ser 
                20                  25                  30  

Thr Asn Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Asp Arg Ser Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Gly Lys 
                65                  70                  75  

Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Phe Tyr Pro Pro Ile Val Gly Asp Tyr 
                95                  100                 105 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115     

<210>  13
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of B4

<400>  13
Gly Phe Thr Phe Ser Ser Tyr Tyr 
1               5               

<210>  14
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of B4

<400>  14
Ile Tyr Ala Asp Gly Asp Met Thr 
1               5               

<210>  15
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of B4

<400>  15
Ala Lys Asp Pro Leu Pro Pro Tyr His 
1               5                   

<210>  16
<211>  116
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B4

<400>  16
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Val Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser 
                20                  25                  30  

Ser Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                35                  40                  45  

Glu Trp Val Ala Ser Ile Tyr Ala Asp Gly Asp Met Thr Tyr Tyr 
                50                  55                  60  

Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                65                  70                  75  

Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp 
                80                  85                  90  

Thr Ala Val Tyr Tyr Cys Ala Lys Asp Pro Leu Pro Pro Tyr His 
                95                  100                 105 

Val Asn Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115     

<210>  17
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B1 with HA-His6-tag

<400>  17
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Leu Ser Ser 
                20                  25                  30  

Ser Asn Ser Met Ala Trp Tyr Arg Gln Val Pro Gly Lys Arg Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Gly Glu Thr Asn Tyr Ala 
                50                  55                  60  

Asp Phe Val Gly Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Gly Leu Tyr Leu Gln Leu Asn Gly Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Ala Tyr Tyr Cys Asn Phe Trp Pro Pro Leu Ile Asn Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala Tyr Pro 
                110                 115                 120 

Tyr Asp Val Pro Asp Tyr Gly Ser His His His His His His 
                125                 130                 

<210>  18
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B2 with HA-His6-tag

<400>  18
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Ser Ile Ser Ser 
                20                  25                  30  

Ala Asn Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Asp Val Val Ala Gly Leu Thr Thr Gly Gly Arg Ser His Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Leu Trp Pro Pro Val Gln Gly Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala Tyr Pro 
                110                 115                 120 

Tyr Asp Val Pro Asp Tyr Gly Ser His His His His His His 
                125                 130                 

<210>  19
<211>  135
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B3 with HA-His6-tag

<400>  19
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Leu Phe Ser 
                20                  25                  30  

Thr Asn Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Asp Arg Ser Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Gly Lys 
                65                  70                  75  

Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Phe Tyr Pro Pro Ile Val Gly Asp Tyr 
                95                  100                 105 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala Tyr 
                110                 115                 120 

Pro Tyr Asp Val Pro Asp Tyr Gly Ser His His His His His His 
                125                 130                 135 




<210>  20
<211>  135
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B4 with HA-His6-tag

<400>  20
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Val Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser 
                20                  25                  30  

Ser Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                35                  40                  45  

Glu Trp Val Ala Ser Ile Tyr Ala Asp Gly Asp Met Thr Tyr Tyr 
                50                  55                  60  

Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                65                  70                  75  

Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp 
                80                  85                  90  

Thr Ala Val Tyr Tyr Cys Ala Lys Asp Pro Leu Pro Pro Tyr His 
                95                  100                 105 

Val Asn Gln Gly Thr Gln Val Thr Val Ser Ser Ala Ala Ala Tyr 
                110                 115                 120 

Pro Tyr Asp Val Pro Asp Tyr Gly Ser His His His His His His 
                125                 130                 135 




<210>  21
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B1 with His6-tag

<400>  21
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Leu Ser Ser 
                20                  25                  30  

Ser Asn Ser Met Ala Trp Tyr Arg Gln Val Pro Gly Lys Arg Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Gly Glu Thr Asn Tyr Ala 
                50                  55                  60  

Asp Phe Val Gly Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Gly Leu Tyr Leu Gln Leu Asn Gly Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Ala Tyr Tyr Cys Asn Phe Trp Pro Pro Leu Ile Asn Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
                110                 115                 120 

His 
    

<210>  22
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B2 with His6-tag

<400>  22
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Ser Ile Ser Ser 
                20                  25                  30  

Ala Asn Ser Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Asp Val Val Ala Gly Leu Thr Thr Gly Gly Arg Ser His Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Leu Trp Pro Pro Val Gln Gly Tyr Trp 
                95                  100                 105 

Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
                110                 115                 120 

His 
    

<210>  23
<211>  122
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B3 with His6-tag

<400>  23
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Leu Phe Ser 
                20                  25                  30  

Thr Asn Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Val Ala Gly Ile Thr Gly Gly Asp Arg Ser Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Gly Lys 
                65                  70                  75  

Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Phe Tyr Pro Pro Ile Val Gly Asp Tyr 
                95                  100                 105 

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
                110                 115                 120 

His His 
        

<210>  24
<211>  122
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B4 with His6-tag

<400>  24
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Val Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Phe Thr Phe Ser 
                20                  25                  30  

Ser Tyr Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
                35                  40                  45  

Glu Trp Val Ala Ser Ile Tyr Ala Asp Gly Asp Met Thr Tyr Tyr 
                50                  55                  60  

Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                65                  70                  75  

Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp 
                80                  85                  90  

Thr Ala Val Tyr Tyr Cys Ala Lys Asp Pro Leu Pro Pro Tyr His 
                95                  100                 105 

Val Asn Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
                110                 115                 120 

His His 
        

<210>  25
<211>  2283
<212>  DNA
<213>  Homo sapiens

<220>
<223>  nucleic acid encoding human fibroblast activation protein alpha (FAP), transcript variant 1

<400>  25
atgaagactt gggtaaaaat cgtatttgga gttgccacct ctgctgtgct                 50
tgccttattg gtgatgtgca ttgtcttacg cccttcaaga gttcataact                 100
ctgaagaaaa tacaatgaga gcactcacac tgaaggatat tttaaatgga                 150
acattttctt ataaaacatt ttttccaaac tggatttcag gacaagaata                 200
tcttcatcaa tctgcagata acaatatagt actttataat attgaaacag                 250
gacaatcata taccattttg agtaatagaa ccatgaaaag tgtgaatgct                 300
tcaaattacg gcttatcacc tgatcggcaa tttgtatatc tagaaagtga                 350
ttattcaaag ctttggagat actcttacac agcaacatat tacatctatg                 400
accttagcaa tggagaattt gtaagaggaa atgagcttcc tcgtccaatt                 450
cagtatttat gctggtcgcc tgttgggagt aaattagcat atgtctatca                 500
aaacaatatc tatttgaaac aaagaccagg agatccacct tttcaaataa                 550
catttaatgg aagagaaaat aaaatattta atggaatccc agactgggtt                 600
tatgaagagg aaatgcttgc tacaaaatat gctctctggt ggtctcctaa                 650
tggaaaattt ttggcatatg cggaatttaa tgatacggat ataccagtta                 700
ttgcctattc ctattatggc gatgaacaat atcctagaac aataaatatt                 750
ccatacccaa aggctggagc taagaatccc gttgttcgga tatttattat                 800
cgataccact taccctgcgt atgtaggtcc ccaggaagtg cctgttccag                 850
caatgatagc ctcaagtgat tattatttca gttggctcac gtgggttact                 900
gatgaacgag tatgtttgca gtggctaaaa agagtccaga atgtttcggt                 950
cctgtctata tgtgacttca gggaagactg gcagacatgg gattgtccaa                 1000
agacccagga gcatatagaa gaaagcagaa ctggatgggc tggtggattc                 1050
tttgtttcaa caccagtttt cagctatgat gccatttcgt actacaaaat                 1100
atttagtgac aaggatggct acaaacatat tcactatatc aaagacactg                 1150
tggaaaatgc tattcaaatt acaagtggca agtgggaggc cataaatata                 1200
ttcagagtaa cacaggattc actgttttat tctagcaatg aatttgaaga                 1250
ataccctgga agaagaaaca tctacagaat tagcattgga agctatcctc                 1300
caagcaagaa gtgtgttact tgccatctaa ggaaagaaag gtgccaatat                 1350
tacacagcaa gtttcagcga ctacgccaag tactatgcac ttgtctgcta                 1400
cggcccaggc atccccattt ccacccttca tgatggacgc actgatcaag                 1450
aaattaaaat cctggaagaa aacaaggaat tggaaaatgc tttgaaaaat                 1500
atccagctgc ctaaagagga aattaagaaa cttgaagtag atgaaattac                 1550
tttatggtac aagatgattc ttcctcctca atttgacaga tcaaagaagt                 1600
atcccttgct aattcaagtg tatggtggtc cctgcagtca gagtgtaagg                 1650
tctgtatttg ctgttaattg gatatcttat cttgcaagta aggaagggat                 1700
ggtcattgcc ttggtggatg gtcgaggaac agctttccaa ggtgacaaac                 1750
tcctctatgc agtgtatcga aagctgggtg tttatgaagt tgaagaccag                 1800
attacagctg tcagaaaatt catagaaatg ggtttcattg atgaaaaaag                 1850
aatagccata tggggctggt cctatggagg atacgtttca tcactggccc                 1900
ttgcatctgg aactggtctt ttcaaatgtg gtatagcagt ggctccagtc                 1950
tccagctggg aatattacgc gtctgtctac acagagagat tcatgggtct                 2000
cccaacaaag gatgataatc ttgagcacta taagaattca actgtgatgg                 2050
caagagcaga atatttcaga aatgtagact atcttctcat ccacggaaca                 2100
gcagatgata atgtgcactt tcaaaactca gcacagattg ctaaagctct                 2150
ggttaatgca caagtggatt tccaggcaat gtggtactct gaccagaacc                 2200
acggcttatc cggcctgtcc acgaaccact tatacaccca catgacccac                 2250
ttcctaaagc agtgtttctc tttgtcagac taa                                   2283

<210>  26
<211>  760
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human fibroblast activation protein alpha (FAP), transcript variant 1

<400>  26
Met Lys Thr Trp Val Lys Ile Val Phe Gly Val Ala Thr Ser Ala 
1               5                   10                  15  

Val Leu Ala Leu Leu Val Met Cys Ile Val Leu Arg Pro Ser Arg 
                20                  25                  30  

Val His Asn Ser Glu Glu Asn Thr Met Arg Ala Leu Thr Leu Lys 
                35                  40                  45  

Asp Ile Leu Asn Gly Thr Phe Ser Tyr Lys Thr Phe Phe Pro Asn 
                50                  55                  60  

Trp Ile Ser Gly Gln Glu Tyr Leu His Gln Ser Ala Asp Asn Asn 
                65                  70                  75  

Ile Val Leu Tyr Asn Ile Glu Thr Gly Gln Ser Tyr Thr Ile Leu 
                80                  85                  90  

Ser Asn Arg Thr Met Lys Ser Val Asn Ala Ser Asn Tyr Gly Leu 
                95                  100                 105 

Ser Pro Asp Arg Gln Phe Val Tyr Leu Glu Ser Asp Tyr Ser Lys 
                110                 115                 120 

Leu Trp Arg Tyr Ser Tyr Thr Ala Thr Tyr Tyr Ile Tyr Asp Leu 
                125                 130                 135 

Ser Asn Gly Glu Phe Val Arg Gly Asn Glu Leu Pro Arg Pro Ile 
                140                 145                 150 

Gln Tyr Leu Cys Trp Ser Pro Val Gly Ser Lys Leu Ala Tyr Val 
                155                 160                 165 

Tyr Gln Asn Asn Ile Tyr Leu Lys Gln Arg Pro Gly Asp Pro Pro 
                170                 175                 180 

Phe Gln Ile Thr Phe Asn Gly Arg Glu Asn Lys Ile Phe Asn Gly 
                185                 190                 195 

Ile Pro Asp Trp Val Tyr Glu Glu Glu Met Leu Ala Thr Lys Tyr 
                200                 205                 210 

Ala Leu Trp Trp Ser Pro Asn Gly Lys Phe Leu Ala Tyr Ala Glu 
                215                 220                 225 

Phe Asn Asp Thr Asp Ile Pro Val Ile Ala Tyr Ser Tyr Tyr Gly 
                230                 235                 240 

Asp Glu Gln Tyr Pro Arg Thr Ile Asn Ile Pro Tyr Pro Lys Ala 
                245                 250                 255 

Gly Ala Lys Asn Pro Val Val Arg Ile Phe Ile Ile Asp Thr Thr 
                260                 265                 270 

Tyr Pro Ala Tyr Val Gly Pro Gln Glu Val Pro Val Pro Ala Met 
                275                 280                 285 

Ile Ala Ser Ser Asp Tyr Tyr Phe Ser Trp Leu Thr Trp Val Thr 
                290                 295                 300 

Asp Glu Arg Val Cys Leu Gln Trp Leu Lys Arg Val Gln Asn Val 
                305                 310                 315 

Ser Val Leu Ser Ile Cys Asp Phe Arg Glu Asp Trp Gln Thr Trp 
                320                 325                 330 

Asp Cys Pro Lys Thr Gln Glu His Ile Glu Glu Ser Arg Thr Gly 
                335                 340                 345 

Trp Ala Gly Gly Phe Phe Val Ser Thr Pro Val Phe Ser Tyr Asp 
                350                 355                 360 

Ala Ile Ser Tyr Tyr Lys Ile Phe Ser Asp Lys Asp Gly Tyr Lys 
                365                 370                 375 

His Ile His Tyr Ile Lys Asp Thr Val Glu Asn Ala Ile Gln Ile 
                380                 385                 390 

Thr Ser Gly Lys Trp Glu Ala Ile Asn Ile Phe Arg Val Thr Gln 
                395                 400                 405 

Asp Ser Leu Phe Tyr Ser Ser Asn Glu Phe Glu Glu Tyr Pro Gly 
                410                 415                 420 

Arg Arg Asn Ile Tyr Arg Ile Ser Ile Gly Ser Tyr Pro Pro Ser 
                425                 430                 435 

Lys Lys Cys Val Thr Cys His Leu Arg Lys Glu Arg Cys Gln Tyr 
                440                 445                 450 

Tyr Thr Ala Ser Phe Ser Asp Tyr Ala Lys Tyr Tyr Ala Leu Val 
                455                 460                 465 

Cys Tyr Gly Pro Gly Ile Pro Ile Ser Thr Leu His Asp Gly Arg 
                470                 475                 480 

Thr Asp Gln Glu Ile Lys Ile Leu Glu Glu Asn Lys Glu Leu Glu 
                485                 490                 495 

Asn Ala Leu Lys Asn Ile Gln Leu Pro Lys Glu Glu Ile Lys Lys 
                500                 505                 510 

Leu Glu Val Asp Glu Ile Thr Leu Trp Tyr Lys Met Ile Leu Pro 
                515                 520                 525 

Pro Gln Phe Asp Arg Ser Lys Lys Tyr Pro Leu Leu Ile Gln Val 
                530                 535                 540 

Tyr Gly Gly Pro Cys Ser Gln Ser Val Arg Ser Val Phe Ala Val 
                545                 550                 555 

Asn Trp Ile Ser Tyr Leu Ala Ser Lys Glu Gly Met Val Ile Ala 
                560                 565                 570 

Leu Val Asp Gly Arg Gly Thr Ala Phe Gln Gly Asp Lys Leu Leu 
                575                 580                 585 

Tyr Ala Val Tyr Arg Lys Leu Gly Val Tyr Glu Val Glu Asp Gln 
                590                 595                 600 

Ile Thr Ala Val Arg Lys Phe Ile Glu Met Gly Phe Ile Asp Glu 
                605                 610                 615 

Lys Arg Ile Ala Ile Trp Gly Trp Ser Tyr Gly Gly Tyr Val Ser 
                620                 625                 630 

Ser Leu Ala Leu Ala Ser Gly Thr Gly Leu Phe Lys Cys Gly Ile 
                635                 640                 645 

Ala Val Ala Pro Val Ser Ser Trp Glu Tyr Tyr Ala Ser Val Tyr 
                650                 655                 660 

Thr Glu Arg Phe Met Gly Leu Pro Thr Lys Asp Asp Asn Leu Glu 
                665                 670                 675 

His Tyr Lys Asn Ser Thr Val Met Ala Arg Ala Glu Tyr Phe Arg 
                680                 685                 690 

Asn Val Asp Tyr Leu Leu Ile His Gly Thr Ala Asp Asp Asn Val 
                695                 700                 705 

His Phe Gln Asn Ser Ala Gln Ile Ala Lys Ala Leu Val Asn Ala 
                710                 715                 720 

Gln Val Asp Phe Gln Ala Met Trp Tyr Ser Asp Gln Asn His Gly 
                725                 730                 735 

Leu Ser Gly Leu Ser Thr Asn His Leu Tyr Thr His Met Thr His 
                740                 745                 750 

Phe Leu Lys Gln Cys Phe Ser Leu Ser Asp 
                755                 760 

<210>  27
<211>  17086
<212>  DNA
<213>  Artificial sequence

<220>
<223>  targeting vector

<400>  27
cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc                 50
gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt                 100
gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg                 150
ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat                 200
tggagctcca ccgcccgggc tggttctttc cgcctcagaa gccatagagc                 250
ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg                 300
ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca                 350
atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac                 400
cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg                 450
caactagaag gcacagtcga ggctgatcag cgagctctag gatctgcatt                 500
ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac                 550
aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata                 600
tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac                 650
accacagaag taaggttcct tcacaaagag atcgcctgac acgatttcct                 700
gcacaggctt gagccatata ctcatacatc gcatcttggc cacgttttcc                 750
acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt                 800
cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg                 850
ctgagcacta cacgcgaagc accatcaccg aaccttttga taaactcttc                 900
cgttccgact tgctccatca acggttcagt gagacttaaa cctaactctt                 950
tcttaatagt ttcggcatta tccactttta gtgcgagaac cttcgtcagt                 1000
cctggatacg tcactttgac cacgcctcca gcttttccag agagcgggtt                 1050
ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat                 1100
aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc                 1150
ttttgtatac ctttttgaat ggaatctaca taaccaggtt tagtcccgtg                 1200
gtacgaagaa aagttttcca tcacaaaaga tttagaagaa tcaacaacat                 1250
catcaggatc catggcacgc gcttctacaa ggcgctggcc gaagaggtgc                 1300
gggagtttca cgccaccaag atctgcggca cgctgttgac gctgttaagc                 1350
gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat                 1400
atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg                 1450
aattcgccaa tgacaagacg ctgggcgggg tttgctcgac attgggtgga                 1500
aacattccag gcctgggtgg agaggctttt tgcttcctct tgcaaaacca                 1550
cactgctcga cattgggtgg aaacattcca ggcctgggtg gagaggcttt                 1600
ttgcttcctc ttgaaaacca cactgctcga tttgttagca gcctcgaatc                 1650
aacccgggcg atcctaggcg atgagatcta gctgtcgcga agagtggcgc                 1700
gccacccagt cacatcctcc ttaaaacagt tcaaacagct agaactatcc                 1750
cagatgttct agttagattc aaccagcttt ctaaagaaaa tacaaacata                 1800
aataaaatgt ttattcaacc ttaaaatgtc cccgtatact agtactttca                 1850
acttaatgtt gcttcttaag atgtcccaat ttctccataa aacctgtcag                 1900
acgagtcttt ggagtcctaa gtggtaccca ttaccagtca tgtgacaatg                 1950
attaattgct aaagctagca gtatgcattt taattatacc ttaatcactt                 2000
attgcattta gtttctgtct agtaaaggag gtcttgggaa tcactcacta                 2050
caaaggactt gctgattttt ttgggggtgg ggggtggggg ggtgggcgtt                 2100
ggcgagctgg gacagaagtt ctgagaaagt actatgtgga tgttccaaag                 2150
aaagcaacaa atttgagaag accctctaga gggcagttca catgggaccc                 2200
ggctgatggc tttctctcgg attgacgcct cacaatcaag gcacatggag                 2250
atgtcagtaa gtgcactgta cacctgtgat gatgcctggt actgcttcaa                 2300
tttgtaaatc gatagctcat taaccgcatg gggagtggga ttcatccggg                 2350
agctgcagtg ggctttgaat actcttctct gccaatgcat actagtgact                 2400
cactctgctc accagaactt tcctaaggct agaattcagg gacagctgtc                 2450
tggagaatgg cagggaggaa gttcacaagc cactgtgctg gtttgtggtg                 2500
gagaaagttt cctgtggcag ccagaccagt caactaaagc aaacagcaac                 2550
acagaacagt gatgttttct cgttattttt catgcacagt ttgtgaaaga                 2600
tcagcatgaa acttagcata gtcaggcaaa agatattaca aacttcttga                 2650
gaaagccagc aatggaaaaa ctggagacac catattctag caacagtatc                 2700
catgtggaaa ccaggaatct gccctaggaa attgctctgg accacagaga                 2750
aggtttaaaa aaaaattgtg tctgtgtgtg tgtgtgtgtg tgtgtgtgtg                 2800
ttgtgaattc agtcatttta ctgatctgcc aagatgaccg atcactttga                 2850
aatatgtgga cttagccaaa atgaagtaca catgacttct cttttcactt                 2900
gcctttttaa aatttatttt aaggtcttta taattactaa atgttctcct                 2950
ttctcagaag atattctgag agcaaagcaa aaatatcact cttgtaaagc                 3000
catttccatt cttccaaagg tctgctggca aattattccc gctgatcttt                 3050
ccatctttct agcctgtgca aacacaccta acccatacta catttcaaca                 3100
gatggcgctt catttcttta aaggaaagca gccgtgggtt tagacagttg                 3150
aatttttaaa cttctgtatt tactgaaagt gcatatggtg ctatacggac                 3200
aaagaaattg tgctgaaaga aaaacatttg tgcctgcagc acctcatagg                 3250
ctcccgggag aagagtgtgc agtcacaccg tgtacctctc acaaagccgg                 3300
atttggctcc actgttcttc ccttccccaa acatttgata tgtacaatgc                 3350
accctcaaaa agtttctcca tttgaaatcc ttcctctcag ctgcaaacaa                 3400
gaggagtgta gaaaagattg ttactaaagt gactcgagtg atttttgagg                 3450
agaatatgaa atataaacct gaacaacaac aaaaaggtga aataaaacgg                 3500
cctctgtatg acaacttaag cagatgttgg tgtaaattta caaggatgag                 3550
agagctctag agcctccctc cagccactca tagttcactt aaaggccaag                 3600
aacgccccca aaatctgttt ctaattttac agaaatcttt cgaaatttgg                 3650
cacggtagtc aaaagtccgt gggaaaggga ggaggggagc gggaagcaac                 3700
tcatgtcctg acttcagctt ccaagtggaa gacagacttg cttcttttca                 3750
acagttttca cagatgcggt gacccacgct gtgcagtgag aatcagctaa                 3800
cttgcaaaaa catctggaaa aatgaagact tgggtaaaaa tcgtatttgg                 3850
agttgccacc tctgctgtgc ttgccttatt ggtgatgtgc attgtcttac                 3900
gcccttcaag agttcataac tctgaagaaa atacaatgag agcactcaca                 3950
ctgaaggata ttttaaatgg aacattttct tataaaacat tttttccaaa                 4000
ctggatttca ggacaagaat atcttcatca atctgcagat aacaatatag                 4050
tactttataa tattgaaaca ggacaatcat ataccatttt gagtaataga                 4100
accatgaaaa gtgtgaatgc ttcaaattac ggcttatcac ctgatcggca                 4150
atttgtatat ctagaaagtg attattcaaa gctttggaga tactcttaca                 4200
cagcaacata ttacatctat gaccttagca atggagaatt tgtaagagga                 4250
aatgagcttc ctcgtccaat tcagtattta tgctggtcgc ctgttgggag                 4300
taaattagca tatgtctatc aaaacaatat ctatttgaaa caaagaccag                 4350
gagatccacc ttttcaaata acatttaatg gaagagaaaa taaaatattt                 4400
aatggaatcc cagactgggt ttatgaagag gaaatgcttg ctacaaaata                 4450
tgctctctgg tggtctccta atggaaaatt tttggcatat gcggaattta                 4500
atgatacgga tataccagtt attgcctatt cctattatgg cgatgaacaa                 4550
tatcctagaa caataaatat tccataccca aaggctggag ctaagaatcc                 4600
cgttgttcgg atatttatta tcgataccac ttaccctgcg tatgtaggtc                 4650
cccaggaagt gcctgttcca gcaatgatag cctcaagtga ttattatttc                 4700
agttggctca cgtgggttac tgatgaacga gtatgtttgc agtggctaaa                 4750
aagagtccag aatgtttcgg tcctgtctat atgtgacttc agggaagact                 4800
ggcagacatg ggattgtcca aagacccagg agcatataga agaaagcaga                 4850
actggatggg ctggtggatt ctttgtttca acaccagttt tcagctatga                 4900
tgccatttcg tactacaaaa tatttagtga caaggatggc tacaaacata                 4950
ttcactatat caaagacact gtggaaaatg ctattcaaat tacaagtggc                 5000
aagtgggagg ccataaatat attcagagta acacaggatt cactgtttta                 5050
ttctagcaat gaatttgaag aataccctgg aagaagaaac atctacagaa                 5100
ttagcattgg aagctatcct ccaagcaaga agtgtgttac ttgccatcta                 5150
aggaaagaaa ggtgccaata ttacacagca agtttcagcg actacgccaa                 5200
gtactatgca cttgtctgct acggcccagg catccccatt tccacccttc                 5250
atgatggacg cactgatcaa gaaattaaaa tcctggaaga aaacaaggaa                 5300
ttggaaaatg ctttgaaaaa tatccagctg cctaaagagg aaattaagaa                 5350
acttgaagta gatgaaatta ctttatggta caagatgatt cttcctcctc                 5400
aatttgacag atcaaagaag tatcccttgc taattcaagt gtatggtggt                 5450
ccctgcagtc agagtgtaag gtctgtattt gctgttaatt ggatatctta                 5500
tcttgcaagt aaggaaggga tggtcattgc cttggtggat ggtcgaggaa                 5550
cagctttcca aggtgacaaa ctcctctatg cagtgtatcg aaagctgggt                 5600
gtttatgaag ttgaagacca gattacagct gtcagaaaat tcatagaaat                 5650
gggtttcatt gatgaaaaaa gaatagccat atggggctgg tcctatggag                 5700
gatacgtttc atcactggcc cttgcatctg gaactggtct tttcaaatgt                 5750
ggtatagcag tggctccagt ctccagctgg gaatattacg cgtctgtcta                 5800
cacagagaga ttcatgggtc tcccaacaaa ggatgataat cttgagcact                 5850
ataagaattc aactgtgatg gcaagagcag aatatttcag aaatgtagac                 5900
tatcttctca tccacggaac agcagatgat aatgtgcact ttcaaaactc                 5950
agcacagatt gctaaagctc tggttaatgc acaagtggat ttccaggcaa                 6000
tgtggtactc tgaccagaac cacggcttat ccggcctgtc cacgaaccac                 6050
ttatacaccc acatgaccca cttcctaaag cagtgtttct ctttgtcaga                 6100
ctaatcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc                 6150
aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa                 6200
ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa                 6250
ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca                 6300
ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta                 6350
tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca                 6400
aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc                 6450
cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat                 6500
attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac                 6550
atgttttact agccagattt ttcctcctct cctgactact cccagtcata                 6600
gctgtccctc ttctcttatg gagatcctcg agggacctaa taacttcgta                 6650
tagcatacat tatacgaagt tatattaagg gttccgcaag ctctagtcga                 6700
gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc                 6750
cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc                 6800
accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat                 6850
ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca                 6900
aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc                 6950
acagtcgagg ctgatcagcg agctctagag aattgatccc ctcagaagaa                 7000
ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga                 7050
taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca                 7100
gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc                 7150
cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga                 7200
tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg                 7250
ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg                 7300
atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag                 7350
tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta                 7400
gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac                 7450
tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt                 7500
cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca                 7550
gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc                 7600
gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa                 7650
ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg                 7700
attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc                 7750
cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatggttt                 7800
agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat                 7850
taattgtcaa caggctgcag gtcgaaaggc ccggagatga ggaagaggag                 7900
aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc                 7950
ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc                 8000
ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa                 8050
agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc                 8100
ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc                 8150
acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg                 8200
ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg                 8250
gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt                 8300
agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt                 8350
gggaaaagcg cctcccctac ccggtagaat ttcgacgacc tgcagccaag                 8400
ctagcttcgc gagctcgacc gaacaaacga cccaacaccc gtgcgtttta                 8450
ttctgtcttt ttattgccgc tcagctttac agtgacaatg acggctggcg                 8500
actgaatatt agtgcttaca gacagcacta catattttcc gtcgatgttg                 8550
aaatcctttc tcatatgtca ccataaatat caaataatta tagcaatcat                 8600
ttacgcgtta atggctaatc gccatcttcc agcaggcgca ccattgcccc                 8650
tgtttcacta tccaggttac ggatatagtt catgacaata tttacattgg                 8700
tccagccacc agcttgcatg atctccggta ttgaaactcc agcgcgggcc                 8750
atatctcgcg cggctccgac acgggcactg tgtccagacc aggccaggta                 8800
tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt                 8850
caaaaatccc ttccagggcg cgagttgata gctggctggt ggcagatggc                 8900
gcggcaacac cattttttct gacccggcaa aacaggtagt tattcggatc                 8950
atcagctaca ccagagacgg aaatccatcg ctcgaccagt ttagttaccc                 9000
ccaggctaag tgccttctct acacctgcgg tgctaaccag cgttttcgtt                 9050
ctgccaatat ggattaacat tctcccaccg tcagtacgtg agatatcttt                 9100
aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa                 9150
tccccagaaa tgccagatta cgtatatcct ggcagcgatc gctattttcc                 9200
atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg                 9250
ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa                 9300
ccagtgaaac agcattgctg tcacttggtc gtggcagccc ggaccgacga                 9350
tgaagcatgt ttagctggcc caaatgttgc tggatagttt ttactgccag                 9400
accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg                 9450
cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg                 9500
caaacggaca gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc                 9550
cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga                 9600
ccggtaatgc aggcaaattt tggtgtacgg tcagtaaatt ggacaccttc                 9650
ctcttcttct tgggcatggc cgcaggaaag cagagccctg aagctcccat                 9700
caccggccaa taagagccaa gcctgcagtg tgacctcata gagcaatgtg                 9750
ccagccagcc tgaccccaag ggccctcagg cttgggcaca ctgtctctag                 9800
gaccctgaga gaaagacata cccatttctg cttagggccc tgaggatgag                 9850
cccaggggtg gcttggcact gaagcaaagg acactggggc tcagctggca                 9900
gcaaagtgac caggatgctg aggctttgac ccagaagcca gaggccagag                 9950
gccaggactt ctcttggtcc cagtccaccc tcactcagag ctttaccaat                 10000
gccctctgga tagttgtcgg gtaacggtgg acgccactga ttctctggcc                 10050
agcctaggac ttcgccattc cgctgattct gctcttccag ccactggctg                 10100
accggttgga agtactccag cagtgccttg gcatccaggg catctgagcc                 10150
taccaggtcc ttcagtacct cctgccaggg cctggagcag ccagcctgca                 10200
acacctgcct gccaagcaga gtgaccactg tgggcacagg ggacacaggg                 10250
tggggcccac aacagcacca ttgtccactt gtccctcact agtaaaagaa                 10300
ctctagggtt gcggggggtg ggggaggtct ctgtgaggct ggtaagggat                 10350
atttgcctgg cccatggagc tagcttggct ggacgtaaac tcctcttcag                 10400
acctaataac ttcgtatagc atacattata cgaagttata ttaagggtta                 10450
ttgaatatga tcggaattgg gctgcaggaa ttcgatagct tggctgcagg                 10500
tcgacactgg cgatgtaagg gtaggaagag ctttacagtg ttatttcagc                 10550
aacagaaaaa cctgagagct attctgtacc cctcccacca cccccccaaa                 10600
atacacgcag aagacaagct ttgataggcc tctcaagact gtatttttaa                 10650
ctagcattgt tttctattac tagatttgcc tggtagcaag aaaaaaaata                 10700
atgagtaaga atagaaacgt taaaaaaaga acctatggag gctttgaaat                 10750
cactaaatag tgcttccaaa aaatttaagg aagatgttca gaatagaaat                 10800
ctcaggaata gccatatagg aaaagtaaaa ctcttgttct atatatagta                 10850
gcaattagtc attcaattaa gaggcttgtt acagttacgc aaacattatc                 10900
aaggtaaatt ggttggggtg tttgtttgtt tgtttgtttg ttttttattt                 10950
ttacagaaag gcttaggctg gcaacaatgt cacttgactg agacctagaa                 11000
gatgttatgc actgattttg aaactagttt taattttaat attatccatt                 11050
ggtaataaac tatgccccta ttattccaaa ccttcttatg aacagcaatg                 11100
tatgcttcta aagcagagat gtaatgtaac agtgacagag actgtactat                 11150
aggatctggg aagaacaaat gctccgagtt gatcagaacc agggaacgtc                 11200
tgtggttaaa gtgaacggac tctgaacaag ggttagtggt ccagcctgaa                 11250
ccagcaaaca cgtcagtcct gaattctctg ttgcttttta aaattgaaac                 11300
atgtacaacg tacaaaagct tgaagagttc aaaaatgtcc acatcctttc                 11350
acagaattgc aatgtgcagg ataacctgat gaaggcagtc tccttggagc                 11400
tgcactttta atttttagct tgcatcttca tgtcctaaag cacaagattt                 11450
cccctgggcc atcaaggtgt atcctcctat tgtcatttta ttaagctaga                 11500
ctctcacctc aattcttaga ggacacactg cctccaacca ggagcttctg                 11550
agtgcctggc caggtctctg acccctttaa tccagtctag tagaaatcag                 11600
cctctggctt ttgcatacac ttaccagagc ttgagatgga tattctgctc                 11650
cctcaaaaat taacagtttt tggaagcaaa agtacatgaa ttttgttgag                 11700
acagactttt ctgtccacaa gtgttgccct ttggactttg aagaatattc                 11750
ccaatgagaa gcatatttat ccattgcaaa tagcatataa ggatagatat                 11800
ttgggtctat acttacattt atagatatag acatttgtgt aattacatat                 11850
aatcccaatc tttatcttct ttccaagttt ttttatgaat agcatactca                 11900
aaaatatgtt ataaactata tataacataa aagtcctgtg tttttttata                 11950
acatagattt ctattttcaa tggaaaagat aattctctaa gtaatttcct                 12000
gttattgtga aataatcaaa ttaacctcaa gatgaattat tttgcattca                 12050
tttacttctt ctgcagaagt aaacaatgct taggcctgct gtctggactg                 12100
taaagtcact gcctcttcat tattacggac acctcagcat gtcacctgag                 12150
cagcattgtc ttcttgtgcc cataccttca ccatgtccag gctggggacc                 12200
aggcccaact ttactcacat ttcttccatg ggaaggaatg aagtaaaaag                 12250
tatgaaaaag taaagacttc ccatgggaag tcaaggaaaa gtagcaatgg                 12300
aacagaaagg agaagaaaaa gctaggtagg cccctatacc acttcgtcag                 12350
aaatgtcacc actgtacgtt tgtctcctgt gcccttgggt tgctagtcca                 12400
ggagctactt gacaaggtaa cagaagtcaa gaaatatcca tctattccat                 12450
tatttataaa ctaaatgtac tgggttcccc aaaatacctc ctatcacttg                 12500
ctgttgctga agttacaaag ttcagtagtc tcaatttccc tcagacaggc                 12550
aaacaaggaa taatttgtca ttctacacac cctaaaacac atggccacag                 12600
ccatcattat ggagaaatgt ctctgtcgct caagtataag tgaaatgaat                 12650
agagttgggg cgtaggggtc ttttcattcc caggtggaag tccacaaaga                 12700
atcagccttc actttagaga agttttgggg gggggggggg agagagcaga                 12750
cacagacagt aagaatgagt ggaagtggag tagtgaatga gaatgcctgt                 12800
ttaactcact tacatattaa aaataaatgt gttgcagctc tttctggaaa                 12850
tcctaaggta tttcattttc attttaattt taatctaaac tgaagacaaa                 12900
catgcctttg tgccctgcag gccatgtggt ccttggcttc tgccccttca                 12950
cctgactgac tccctttatt ttcctgcctc catttattct ctcttgagga                 13000
tttttttttc cttgcaccct tacaccagta tgaagatgga accaggtaaa                 13050
aagtcagcac aatatccctt ggcttccctt tgtctaccct gatgccaatc                 13100
atacagactt tcttgcttca agagatttac aaagatgatg aaaataaaaa                 13150
ccagaactag aaagtttcag aggccttaga attaaaaaaa aaatctctat                 13200
agccttcttt gagtcatgat ttacatttta tgaatatcac cctttcttcc                 13250
tccatttgtt tgtttgtttg tttattggtt tttgtggtac attaccctat                 13300
ggagtgatat ataagcactc aatacatgtt agctataatt ttacacattt                 13350
atttatttta tatgtgtgcc tctgtgtatg tatgtaggca tggttgtaag                 13400
gatgtcagag ggcaatctgt aggggttgat tctttccttc taccatagga                 13450
gttcctgaga ccaaactcag gtagtcaggc ctactggctg tcacctttac                 13500
ttgctaaacc atctcaattt cccaactatg atttttttaa tttcaaaatt                 13550
tatcacacaa aagaaattgt gcctacaact ctctctcttt tcttttacct                 13600
cctaatatgg ggatctatat gtgataaaat atgtttctta gacttttctg                 13650
tttggtatat taatttttta aacattttct cctgaattct ggtcgtagtc                 13700
accattactc ttgagtgtta ccctatacta tactcggcct gcttggacct                 13750
aaagcaataa atagtttaga aacgtgtgtg tgtgtgtgtg tgtgtgtgtg                 13800
agagagagag agagagagag agagagagag agagagacag agacagagac                 13850
agagacagag acagagagac acagagacaa agagagggag ccttaaaata                 13900
aaaaaaaaca cagaaagaaa aggggagttg ggggcagaaa gataagggga                 13950
gttggggtgt gaagatgaag aagagaaagc aaaaaggaca caccatgtgg                 14000
aagatcacag agtctctgga agtgtgactc tggtgcttcc taaggaagtt                 14050
caggcactgc cactttagcc ttgagccatc ccgactttct cttctctgcc                 14100
tttgcactct cttcctctgt tggccatagc tgtcaatttg gcttaataca                 14150
ttttctggaa atttgtattg gatgccaatt ttaaagtcag atttcacatt                 14200
aaaaaaatga agacttgggc tgttttacaa agtacttgcc acagcagctt                 14250
cttattccct aggtctgaaa gtagtctgtt ctggccgcca ggacaacttg                 14300
aacagaactt tattttctca cagttctgag gtttggaagt ctaagatcaa                 14350
agtgccaact tgtttcttcc tgtagactgc ctttggctta caggccactg                 14400
tatccttgct agacctttcc cttatggcgg ccgcgtacca gcttttgttc                 14450
cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt                 14500
ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc                 14550
ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac                 14600
attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt                 14650
gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt                 14700
attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt                 14750
tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat                 14800
ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag                 14850
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag                 14900
gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt                 14950
ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc                 15000
tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc                 15050
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta                 15100
ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac                 15150
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct                 15200
tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg                 15250
gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg                 15300
aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg                 15350
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat                 15400
ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag                 15450
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc                 15500
tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg                 15550
tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa                 15600
tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag                 15650
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc                 15700
gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg                 15750
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg                 15800
ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg                 15850
agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat                 15900
tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa                 15950
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta                 16000
tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc                 16050
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt                 16100
cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc                 16150
ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt                 16200
gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg                 16250
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt                 16300
taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg                 16350
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa                 16400
ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa                 16450
caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt                 16500
tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg                 16550
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac                 16600
aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc                 16650
tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac                 16700
cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt                 16750
cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg                 16800
ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa                 16850
acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg                 16900
tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg                 16950
ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt                 17000
ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt                 17050
aacaaaaatt taacgcgaat tttaacaaaa tattaa                                17086

<210>  28
<211>  745
<212>  PRT
<213>  Artificial sequence

<220>
<223>  human FAP recombinant protein

<400>  28
Gly Ser His His His His His His Gly Ser Leu Arg Pro Ser Arg 
1               5                   10                  15  

Val His Asn Ser Glu Glu Asn Thr Met Arg Ala Leu Thr Leu Lys 
                20                  25                  30  

Asp Ile Leu Asn Gly Thr Phe Ser Tyr Lys Thr Phe Phe Pro Asn 
                35                  40                  45  

Trp Ile Ser Gly Gln Glu Tyr Leu His Gln Ser Ala Asp Asn Asn 
                50                  55                  60  

Ile Val Leu Tyr Asn Ile Glu Thr Gly Gln Ser Tyr Thr Ile Leu 
                65                  70                  75  

Ser Asn Arg Thr Met Lys Ser Val Asn Ala Ser Asn Tyr Gly Leu 
                80                  85                  90  

Ser Pro Asp Arg Gln Phe Val Tyr Leu Glu Ser Asp Tyr Ser Lys 
                95                  100                 105 

Leu Trp Arg Tyr Ser Tyr Thr Ala Thr Tyr Tyr Ile Tyr Asp Leu 
                110                 115                 120 

Ser Asn Gly Glu Phe Val Arg Gly Asn Glu Leu Pro Arg Pro Ile 
                125                 130                 135 

Gln Tyr Leu Cys Trp Ser Pro Val Gly Ser Lys Leu Ala Tyr Val 
                140                 145                 150 

Tyr Gln Asn Asn Ile Tyr Leu Lys Gln Arg Pro Gly Asp Pro Pro 
                155                 160                 165 

Phe Gln Ile Thr Phe Asn Gly Arg Glu Asn Lys Ile Phe Asn Gly 
                170                 175                 180 

Ile Pro Asp Trp Val Tyr Glu Glu Glu Met Leu Ala Thr Lys Tyr 
                185                 190                 195 

Ala Leu Trp Trp Ser Pro Asn Gly Lys Phe Leu Ala Tyr Ala Glu 
                200                 205                 210 

Phe Asn Asp Thr Asp Ile Pro Val Ile Ala Tyr Ser Tyr Tyr Gly 
                215                 220                 225 

Asp Glu Gln Tyr Pro Arg Thr Ile Asn Ile Pro Tyr Pro Lys Ala 
                230                 235                 240 

Gly Ala Lys Asn Pro Val Val Arg Ile Phe Ile Ile Asp Thr Thr 
                245                 250                 255 

Tyr Pro Ala Tyr Val Gly Pro Gln Glu Val Pro Val Pro Ala Met 
                260                 265                 270 

Ile Ala Ser Ser Asp Tyr Tyr Phe Ser Trp Leu Thr Trp Val Thr 
                275                 280                 285 

Asp Glu Arg Val Cys Leu Gln Trp Leu Lys Arg Val Gln Asn Val 
                290                 295                 300 

Ser Val Leu Ser Ile Cys Asp Phe Arg Glu Asp Trp Gln Thr Trp 
                305                 310                 315 

Asp Cys Pro Lys Thr Gln Glu His Ile Glu Glu Ser Arg Thr Gly 
                320                 325                 330 

Trp Ala Gly Gly Phe Phe Val Ser Thr Pro Val Phe Ser Tyr Asp 
                335                 340                 345 

Ala Ile Ser Tyr Tyr Lys Ile Phe Ser Asp Lys Asp Gly Tyr Lys 
                350                 355                 360 

His Ile His Tyr Ile Lys Asp Thr Val Glu Asn Ala Ile Gln Ile 
                365                 370                 375 

Thr Ser Gly Lys Trp Glu Ala Ile Asn Ile Phe Arg Val Thr Gln 
                380                 385                 390 

Asp Ser Leu Phe Tyr Ser Ser Asn Glu Phe Glu Glu Tyr Pro Gly 
                395                 400                 405 

Arg Arg Asn Ile Tyr Arg Ile Ser Ile Gly Ser Tyr Pro Pro Ser 
                410                 415                 420 

Lys Lys Cys Val Thr Cys His Leu Arg Lys Glu Arg Cys Gln Tyr 
                425                 430                 435 

Tyr Thr Ala Ser Phe Ser Asp Tyr Ala Lys Tyr Tyr Ala Leu Val 
                440                 445                 450 

Cys Tyr Gly Pro Gly Ile Pro Ile Ser Thr Leu His Asp Gly Arg 
                455                 460                 465 

Thr Asp Gln Glu Ile Lys Ile Leu Glu Glu Asn Lys Glu Leu Glu 
                470                 475                 480 

Asn Ala Leu Lys Asn Ile Gln Leu Pro Lys Glu Glu Ile Lys Lys 
                485                 490                 495 

Leu Glu Val Asp Glu Ile Thr Leu Trp Tyr Lys Met Ile Leu Pro 
                500                 505                 510 

Pro Gln Phe Asp Arg Ser Lys Lys Tyr Pro Leu Leu Ile Gln Val 
                515                 520                 525 

Tyr Gly Gly Pro Cys Ser Gln Ser Val Arg Ser Val Phe Ala Val 
                530                 535                 540 

Asn Trp Ile Ser Tyr Leu Ala Ser Lys Glu Gly Met Val Ile Ala 
                545                 550                 555 

Leu Val Asp Gly Arg Gly Thr Ala Phe Gln Gly Asp Lys Leu Leu 
                560                 565                 570 

Tyr Ala Val Tyr Arg Lys Leu Gly Val Tyr Glu Val Glu Asp Gln 
                575                 580                 585 

Ile Thr Ala Val Arg Lys Phe Ile Glu Met Gly Phe Ile Asp Glu 
                590                 595                 600 

Lys Arg Ile Ala Ile Trp Gly Trp Ser Tyr Gly Gly Tyr Val Ser 
                605                 610                 615 

Ser Leu Ala Leu Ala Ser Gly Thr Gly Leu Phe Lys Cys Gly Ile 
                620                 625                 630 

Ala Val Ala Pro Val Ser Ser Trp Glu Tyr Tyr Ala Ser Val Tyr 
                635                 640                 645 

Thr Glu Arg Phe Met Gly Leu Pro Thr Lys Asp Asp Asn Leu Glu 
                650                 655                 660 

His Tyr Lys Asn Ser Thr Val Met Ala Arg Ala Glu Tyr Phe Arg 
                665                 670                 675 

Asn Val Asp Tyr Leu Leu Ile His Gly Thr Ala Asp Asp Asn Val 
                680                 685                 690 

His Phe Gln Asn Ser Ala Gln Ile Ala Lys Ala Leu Val Asn Ala 
                695                 700                 705 

Gln Val Asp Phe Gln Ala Met Trp Tyr Ser Asp Gln Asn His Gly 
                710                 715                 720 

Leu Ser Gly Leu Ser Thr Asn His Leu Tyr Thr His Met Thr His 
                725                 730                 735 

Phe Leu Lys Gln Cys Phe Ser Leu Ser Asp 
                740                 745 

<210>  29
<211>  746
<212>  PRT
<213>  Artificial sequence

<220>
<223>  murine FAP recombinant protein

<400>  29
Gly Ser His His His His His His Gly Ser Leu Arg Pro Ser Arg 
1               5                   10                  15  

Val Tyr Lys Pro Glu Gly Asn Thr Lys Arg Ala Leu Thr Leu Lys 
                20                  25                  30  

Asp Ile Leu Asn Gly Thr Phe Ser Tyr Lys Thr Tyr Phe Pro Asn 
                35                  40                  45  

Trp Ile Ser Glu Gln Glu Tyr Leu His Gln Ser Glu Asp Asp Asn 
                50                  55                  60  

Ile Val Phe Tyr Asn Ile Glu Thr Arg Glu Ser Tyr Ile Ile Leu 
                65                  70                  75  

Ser Asn Ser Thr Met Lys Ser Val Asn Ala Thr Asp Tyr Gly Leu 
                80                  85                  90  

Ser Pro Asp Arg Gln Phe Val Tyr Leu Glu Ser Asp Tyr Ser Lys 
                95                  100                 105 

Leu Trp Arg Tyr Ser Tyr Thr Ala Thr Tyr Tyr Ile Tyr Asp Leu 
                110                 115                 120 

Gln Asn Gly Glu Phe Val Arg Gly Tyr Glu Leu Pro Arg Pro Ile 
                125                 130                 135 

Gln Tyr Leu Cys Trp Ser Pro Val Gly Ser Lys Leu Ala Tyr Val 
                140                 145                 150 

Tyr Gln Asn Asn Ile Tyr Leu Lys Gln Arg Pro Gly Asp Pro Pro 
                155                 160                 165 

Phe Gln Ile Thr Tyr Thr Gly Arg Glu Asn Arg Ile Phe Asn Gly 
                170                 175                 180 

Ile Pro Asp Trp Val Tyr Glu Glu Glu Met Leu Ala Thr Lys Tyr 
                185                 190                 195 

Ala Leu Trp Trp Ser Pro Asp Gly Lys Phe Leu Ala Tyr Val Glu 
                200                 205                 210 

Phe Asn Asp Ser Asp Ile Pro Ile Ile Ala Tyr Ser Tyr Tyr Gly 
                215                 220                 225 

Asp Gly Gln Tyr Pro Arg Thr Ile Asn Ile Pro Tyr Pro Lys Ala 
                230                 235                 240 

Gly Ala Lys Asn Pro Val Val Arg Val Phe Ile Val Asp Thr Thr 
                245                 250                 255 

Tyr Pro His His Val Gly Pro Met Glu Val Pro Val Pro Glu Met 
                260                 265                 270 

Ile Ala Ser Ser Asp Tyr Tyr Phe Ser Trp Leu Thr Trp Val Ser 
                275                 280                 285 

Ser Glu Arg Val Cys Leu Gln Trp Leu Lys Arg Val Gln Asn Val 
                290                 295                 300 

Ser Val Leu Ser Ile Cys Asp Phe Arg Glu Asp Trp His Ala Trp 
                305                 310                 315 

Glu Cys Pro Lys Asn Gln Glu His Val Glu Glu Ser Arg Thr Gly 
                320                 325                 330 

Trp Ala Gly Gly Phe Phe Val Ser Thr Pro Ala Phe Ser Gln Asp 
                335                 340                 345 

Ala Thr Ser Tyr Tyr Lys Ile Phe Ser Asp Lys Asp Gly Tyr Lys 
                350                 355                 360 

His Ile His Tyr Ile Lys Asp Thr Val Glu Asn Ala Ile Gln Ile 
                365                 370                 375 

Thr Ser Gly Lys Trp Glu Ala Ile Tyr Ile Phe Arg Val Thr Gln 
                380                 385                 390 

Asp Ser Leu Phe Tyr Ser Ser Asn Glu Phe Glu Gly Tyr Pro Gly 
                395                 400                 405 

Arg Arg Asn Ile Tyr Arg Ile Ser Ile Gly Asn Ser Pro Pro Ser 
                410                 415                 420 

Lys Lys Cys Val Thr Cys His Leu Arg Lys Glu Arg Cys Gln Tyr 
                425                 430                 435 

Tyr Thr Ala Ser Phe Ser Tyr Lys Ala Lys Tyr Tyr Ala Leu Val 
                440                 445                 450 

Cys Tyr Gly Pro Gly Leu Pro Ile Ser Thr Leu His Asp Gly Arg 
                455                 460                 465 

Thr Asp Gln Glu Ile Gln Val Leu Glu Glu Asn Lys Glu Leu Glu 
                470                 475                 480 

Asn Ser Leu Arg Asn Ile Gln Leu Pro Lys Val Glu Ile Lys Lys 
                485                 490                 495 

Leu Lys Asp Gly Gly Leu Thr Phe Trp Tyr Lys Met Ile Leu Pro 
                500                 505                 510 

Pro Gln Phe Asp Arg Ser Lys Lys Tyr Pro Leu Leu Ile Gln Val 
                515                 520                 525 

Tyr Gly Gly Pro Cys Ser Gln Ser Val Lys Ser Val Phe Ala Val 
                530                 535                 540 

Asn Trp Ile Thr Tyr Leu Ala Ser Lys Glu Gly Ile Val Ile Ala 
                545                 550                 555 

Leu Val Asp Gly Arg Gly Thr Ala Phe Gln Gly Asp Lys Phe Leu 
                560                 565                 570 

His Ala Val Tyr Arg Lys Leu Gly Val Tyr Glu Val Glu Asp Gln 
                575                 580                 585 

Leu Thr Ala Val Arg Lys Phe Ile Glu Met Gly Phe Ile Asp Glu 
                590                 595                 600 

Glu Arg Ile Ala Ile Trp Gly Trp Ser Tyr Gly Gly Tyr Val Ser 
                605                 610                 615 

Ser Leu Ala Leu Ala Ser Gly Thr Gly Leu Phe Lys Cys Gly Ile 
                620                 625                 630 

Ala Val Ala Pro Val Ser Ser Trp Glu Tyr Tyr Ala Ser Ile Tyr 
                635                 640                 645 

Ser Glu Arg Phe Met Gly Leu Pro Thr Lys Asp Asp Asn Leu Glu 
                650                 655                 660 

His Tyr Lys Asn Ser Thr Val Met Ala Arg Ala Glu Tyr Phe Arg 
                665                 670                 675 

Asn Val Asp Tyr Leu Leu Ile His Gly Thr Ala Asp Asp Asn Val 
                680                 685                 690 

His Phe Gln Asn Ser Ala Gln Ile Ala Lys Ala Leu Val Asn Ala 
                695                 700                 705 

Gln Val Asp Phe Gln Ala Met Trp Tyr Ser Asp Gln Asn His Gly 
                710                 715                 720 

Ile Ser Ser Gly Arg Ser Gln Asn His Leu Tyr Thr His Met Thr 
                725                 730                 735 

His Phe Leu Lys Gln Cys Phe Ser Leu Ser Asp 
                740                 745     

<210>  30
<211>  761
<212>  PRT
<213>  Mus musculus

<220>
<223>  murine fibroblast activation protein alpha (FAP)

<400>  30
Met Lys Thr Trp Leu Lys Thr Val Phe Gly Val Thr Thr Leu Ala 
1               5                   10                  15  

Ala Leu Ala Leu Val Val Ile Cys Ile Val Leu Arg Pro Ser Arg 
                20                  25                  30  

Val Tyr Lys Pro Glu Gly Asn Thr Lys Arg Ala Leu Thr Leu Lys 
                35                  40                  45  

Asp Ile Leu Asn Gly Thr Phe Ser Tyr Lys Thr Tyr Phe Pro Asn 
                50                  55                  60  

Trp Ile Ser Glu Gln Glu Tyr Leu His Gln Ser Glu Asp Asp Asn 
                65                  70                  75  

Ile Val Phe Tyr Asn Ile Glu Thr Arg Glu Ser Tyr Ile Ile Leu 
                80                  85                  90  

Ser Asn Ser Thr Met Lys Ser Val Asn Ala Thr Asp Tyr Gly Leu 
                95                  100                 105 

Ser Pro Asp Arg Gln Phe Val Tyr Leu Glu Ser Asp Tyr Ser Lys 
                110                 115                 120 

Leu Trp Arg Tyr Ser Tyr Thr Ala Thr Tyr Tyr Ile Tyr Asp Leu 
                125                 130                 135 

Gln Asn Gly Glu Phe Val Arg Gly Tyr Glu Leu Pro Arg Pro Ile 
                140                 145                 150 

Gln Tyr Leu Cys Trp Ser Pro Val Gly Ser Lys Leu Ala Tyr Val 
                155                 160                 165 

Tyr Gln Asn Asn Ile Tyr Leu Lys Gln Arg Pro Gly Asp Pro Pro 
                170                 175                 180 

Phe Gln Ile Thr Tyr Thr Gly Arg Glu Asn Arg Ile Phe Asn Gly 
                185                 190                 195 

Ile Pro Asp Trp Val Tyr Glu Glu Glu Met Leu Ala Thr Lys Tyr 
                200                 205                 210 

Ala Leu Trp Trp Ser Pro Asp Gly Lys Phe Leu Ala Tyr Val Glu 
                215                 220                 225 

Phe Asn Asp Ser Asp Ile Pro Ile Ile Ala Tyr Ser Tyr Tyr Gly 
                230                 235                 240 

Asp Gly Gln Tyr Pro Arg Thr Ile Asn Ile Pro Tyr Pro Lys Ala 
                245                 250                 255 

Gly Ala Lys Asn Pro Val Val Arg Val Phe Ile Val Asp Thr Thr 
                260                 265                 270 

Tyr Pro His His Val Gly Pro Met Glu Val Pro Val Pro Glu Met 
                275                 280                 285 

Ile Ala Ser Ser Asp Tyr Tyr Phe Ser Trp Leu Thr Trp Val Ser 
                290                 295                 300 

Ser Glu Arg Val Cys Leu Gln Trp Leu Lys Arg Val Gln Asn Val 
                305                 310                 315 

Ser Val Leu Ser Ile Cys Asp Phe Arg Glu Asp Trp His Ala Trp 
                320                 325                 330 

Glu Cys Pro Lys Asn Gln Glu His Val Glu Glu Ser Arg Thr Gly 
                335                 340                 345 

Trp Ala Gly Gly Phe Phe Val Ser Thr Pro Ala Phe Ser Gln Asp 
                350                 355                 360 

Ala Thr Ser Tyr Tyr Lys Ile Phe Ser Asp Lys Asp Gly Tyr Lys 
                365                 370                 375 

His Ile His Tyr Ile Lys Asp Thr Val Glu Asn Ala Ile Gln Ile 
                380                 385                 390 

Thr Ser Gly Lys Trp Glu Ala Ile Tyr Ile Phe Arg Val Thr Gln 
                395                 400                 405 

Asp Ser Leu Phe Tyr Ser Ser Asn Glu Phe Glu Gly Tyr Pro Gly 
                410                 415                 420 

Arg Arg Asn Ile Tyr Arg Ile Ser Ile Gly Asn Ser Pro Pro Ser 
                425                 430                 435 

Lys Lys Cys Val Thr Cys His Leu Arg Lys Glu Arg Cys Gln Tyr 
                440                 445                 450 

Tyr Thr Ala Ser Phe Ser Tyr Lys Ala Lys Tyr Tyr Ala Leu Val 
                455                 460                 465 

Cys Tyr Gly Pro Gly Leu Pro Ile Ser Thr Leu His Asp Gly Arg 
                470                 475                 480 

Thr Asp Gln Glu Ile Gln Val Leu Glu Glu Asn Lys Glu Leu Glu 
                485                 490                 495 

Asn Ser Leu Arg Asn Ile Gln Leu Pro Lys Val Glu Ile Lys Lys 
                500                 505                 510 

Leu Lys Asp Gly Gly Leu Thr Phe Trp Tyr Lys Met Ile Leu Pro 
                515                 520                 525 

Pro Gln Phe Asp Arg Ser Lys Lys Tyr Pro Leu Leu Ile Gln Val 
                530                 535                 540 

Tyr Gly Gly Pro Cys Ser Gln Ser Val Lys Ser Val Phe Ala Val 
                545                 550                 555 

Asn Trp Ile Thr Tyr Leu Ala Ser Lys Glu Gly Ile Val Ile Ala 
                560                 565                 570 

Leu Val Asp Gly Arg Gly Thr Ala Phe Gln Gly Asp Lys Phe Leu 
                575                 580                 585 

His Ala Val Tyr Arg Lys Leu Gly Val Tyr Glu Val Glu Asp Gln 
                590                 595                 600 

Leu Thr Ala Val Arg Lys Phe Ile Glu Met Gly Phe Ile Asp Glu 
                605                 610                 615 

Glu Arg Ile Ala Ile Trp Gly Trp Ser Tyr Gly Gly Tyr Val Ser 
                620                 625                 630 

Ser Leu Ala Leu Ala Ser Gly Thr Gly Leu Phe Lys Cys Gly Ile 
                635                 640                 645 

Ala Val Ala Pro Val Ser Ser Trp Glu Tyr Tyr Ala Ser Ile Tyr 
                650                 655                 660 

Ser Glu Arg Phe Met Gly Leu Pro Thr Lys Asp Asp Asn Leu Glu 
                665                 670                 675 

His Tyr Lys Asn Ser Thr Val Met Ala Arg Ala Glu Tyr Phe Arg 
                680                 685                 690 

Asn Val Asp Tyr Leu Leu Ile His Gly Thr Ala Asp Asp Asn Val 
                695                 700                 705 

His Phe Gln Asn Ser Ala Gln Ile Ala Lys Ala Leu Val Asn Ala 
                710                 715                 720 

Gln Val Asp Phe Gln Ala Met Trp Tyr Ser Asp Gln Asn His Gly 
                725                 730                 735 

Ile Ser Ser Gly Arg Ser Gln Asn His Leu Tyr Thr His Met Thr 
                740                 745                 750 

His Phe Leu Lys Gln Cys Phe Ser Leu Ser Asp 
                755                 760     

<210>  31
<211>  20
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human Cystatin-S signal peptide

<400>  31
Met Ala Arg Pro Leu Cys Thr Leu Leu Leu Leu Met Ala Thr Leu 
1               5                   10                  15  

Ala Gly Ala Leu Ala 
                20  

<210>  32
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  nontargeting control VHH R3B23

<400>  32
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Asp Thr Gly Tyr 
                20                  25                  30  

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
                35                  40                  45  

Ala Val Ile Asn Ser Asp Ser Gly Val Gly Ser Thr Tyr Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Ile Tyr Tyr Cys Ala Ala Gly His Phe Ser Asp Tyr Val Ser 
                95                  100                 105 

Pro Trp Thr Trp Arg Glu Ile Tyr Arg Tyr Asn Val Trp Gly Gln 
                110                 115                 120 

Gly Thr Gln Val Thr Val Ser Ser His His His His His His 
                125                 130                 

<210>  33
<211>  345
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid encoding B1

<400>  33
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggggggtc                 50
tctgagactc tcctgtgtag cctctggaag gctctccagt agcaattcca                 100
tggcctggta tcgccaggtt ccagggaagc gtcgcgagtt ggtcgcggga                 150
attactggtg gtggtgagac aaactatgca gacttcgtgg gtggccgatt                 200
caccatctcc agagacaacg ccaagaacgg gctgtatctg caattgaacg                 250
gcctgaaacc tgaggacacg gccgcctatt attgtaattt ctggccccca                 300
cttatcaact actggggcca agggacccag gtcaccgtct cctca                      345

<210>  34
<211>  345
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid encoding B2

<400>  34
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagg ctggggggtc                 50
tctgagactc tcctgtgcag tttctggaag catctccagt gccaatagca                 100
tgggctggta ccgccaggct ccagggaagc agcgcgacgt ggtcgcaggt                 150
cttactactg gtggtaggag tcactatgca gactccgtga agggccgatt                 200
caccatctcc agagacaatg ccaagaacac ggtgtatctg caaatgaaca                 250
gcctgaaagc agaggacacg gccgtctatt actgtaattt gtggccgccg                 300
gttcagggct actggggcca ggggacccag gtcaccgtct cctca                      345

<210>  35
<211>  348
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid encoding B3

<400>  35
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagg ctggggggtc                 50
tctgagactc tcctgtgcag tctctggaag actcttcagt accaatgcca                 100
tgggctggta ccgccaggct ccagggaagc agcgcgagtt ggtcgcaggc                 150
attactggtg gtgatagatc aaactatgca gactccgtga agggccgatt                 200
caccatctcc agagacaatg gcaagaacac gctgtatctg caaatgaaca                 250
gcctgaaacc tgaggacacg gccgtctatt actgtaattt ctacccgcct                 300
attgtgggtg actactgggg ccaggggacc caggtcaccg tctcctca                   348

<210>  36
<211>  348
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid encoding B4

<400>  36
gatgtgcagc tggtggagtc tgggggagga ttggtgcagg ttgggggctc                 50
tctgagactc tcctgtgtag cctctggatt caccttcagt agctactaca                 100
tgagctgggt ccgccaggct ccagggaagg ggctggagtg ggtggccagt                 150
atttatgctg acggtgatat gacatactat gcagactccg tgaggggccg                 200
attcaccatc tccagagaca acgccaagaa cacgctgtat ctgcaaatga                 250
acagtctgaa atctgaggac acggccgtgt attactgtgc aaaagatccc                 300
ctccccccct atcatgttaa ccaggggacc caggtcaccg tctcctca                   348

<210>  37
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of B1

<400>  37
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser 
                20                  25  

<210>  38
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of B1

<400>  38
Met Ala Trp Tyr Arg Gln Val Pro Gly Lys Arg Arg Glu Leu Val 
1               5                   10                  15  

Ala Gly 
        

<210>  39
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of B1

<400>  39
Asn Tyr Ala Asp Phe Val Gly Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Gly Leu Tyr Leu Gln Leu Asn Gly Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Ala Tyr Tyr Cys 
                35              

<210>  40
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of B1

<400>  40
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  41
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of B2

<400>  41
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser 
                20                  25  

<210>  42
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of B2

<400>  42
Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Asp Val Val 
1               5                   10                  15  

Ala Gly 
        

<210>  43
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of B2

<400>  43
His Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Ala 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  44
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of B2

<400>  44
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  45
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of B2

<400>  45
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Val Ser 
                20                  25  

<210>  46
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of B2

<400>  46
Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
1               5                   10                  15  

Ala Gly 
        

<210>  47
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of B2

<400>  47
Asn Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Gly Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  48
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of B2

<400>  48
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  49
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of B4

<400>  49
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Val Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Val Ala Ser 
                20                  25  

<210>  50
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of B4

<400>  50
Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
1               5                   10                  15  

Ala Ser 
        

<210>  51
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of B4

<400>  51
Tyr Tyr Ala Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  52
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of B4

<400>  52
Val Asn Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  53
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Exemplary HA tag

<400>  53
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   

<210>  54
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Exemplary HA tag

<400>  54
Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser 
1               5                   10  

<210>  55
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Exemplary cysteine tag

<400>  55
Ser Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Cys 
1               5                   10                  15  






