               SEQUENCE LISTING

<110> John Innes Enterprises Ltd
      Quadram Institute Bioscience
      Imperial College Innovations Ltd

<120> Foodstuffs having improved digestion properties

<130> PC931497WO

<150> GB2014104.0
<151> 2020-09-08

<160> 2

<170> BiSSAP 1.3.6

<210> 1
<211> 3549
<212> DNA
<213> Pisum sativum


<220> 
<223> P. sativum SBE1 cDNA

<400> 1
ccattcttca ccgtacaata catccctatc tataataaac aacaattttc ttctcatcat       60

tatatcattt tattcaatta ttaaatagtt ttattctttc aagaagttaa tgcgaatcat      120

tttttactct gtatgtcatc acacttctct tgcctccttc tactcaattt tttagatctc      180

ttcttctttg caatataaac cctaagctga tagaagaaga tggtttatac aatctcagga      240

attcgatttc ccgttcttcc ttctctgcac aagtcgacct tacgctgcga tcgcagagct      300

tcatctcact ctttttttct caagaacaac tcgtcatcat tctctcggac ctcactttat      360

gcaaagtttt cccgcgattc tgaaaccaaa tcttccacaa ttgctgaatc tgataaagta      420

cttattcctg aagatcaaga taactctgta tccttggcag atcaacttga aaatcctgat      480

ataacctcag aggatgcaca gaacttggag gatttaacca tgaaagatgg gaataagtac      540

aatattgatg aatcaactag cagttataga gaggttggag atgagaaagg ttctgttacg      600

tcatcatcac ttgtagatgt taacaccgac actcaagcca agaagacatc agttcattca      660

gacaagaaag taaaagtaga taaacctaag atcattcctc cacccggtac tgggcagaaa      720

atatatgaga ttgatccact tttgcaagct caccgtcagc atcttgattt ccgttatgga      780

caatacaaaa gaattcggga ggaaattgac aaatatgaag gtggtcttga tgcattttcg      840

cgtgggtacg aaaagtttgg cttcacacgc agtgctacag gcattactta cagggagtgg      900

gcacctggag ctaagtcagc agcattagtt ggagatttca acaattggaa tccaaatgca      960

gatgtaatga ctaaggacgc ttttggtgta tgggagatct tcttgccaaa caatgccgat     1020

ggttcgccac caattcctca tggttctcga gtcaagatcc acatggatac tccctctggg     1080

atcaaggact cgattcctgc ttggatcaaa ttctctgtac aggctcctgg tgaaattcca     1140

tacaatggaa tatactatga tcccccagag gaggaaaagt atgtcttcaa acatccacag     1200

ccaaaacgac cacagtcaat tagaatatat gagtcacaca ttggaatgag tagcccggag     1260

ccaaaaatca acacatatgc gaactttaga gatgatgtac tacctcgcat taaaaaactt     1320

ggctataatg ctgtccagat tatggctatc caagaacatt cttattatgc tagttttggg     1380

taccatgtta ctaatttctt tgcacctagc agtcggtttg gtactccaga agatctcaag     1440

tctctgatag acagagccca tgaactaggc ctgcttgttc tgatggatat tgtacatagc     1500

cattcctcaa ataatacatt ggatggcctg aacatgtttg atggaactga tggtcattac     1560

ttccatcctg gttcacgggg ttatcattgg atgtgggatt ctcgcctttt taactatgga     1620

agctgggaag tactaaggta cctactttca aatgcaagat ggtggctgga tgaatataag     1680

tttgatgggt ttcgatttga tggcgtcaca tcaatgatgt acactcatca tggactgcag     1740

gtatctttta ccggaaatta cagtgagtat tttggtttag caactgatgt tgaggctgtg     1800

gtttacatga tgcttgttaa tgatctcatt cacgggctct tccctgaagc tgtttcaatt     1860

ggtgaagatg tgagtggaat gccaacattc tgccttccga cgcaagatgg tgggattggc     1920

tttaactacc gcttgcatat ggctgttgca gacaagtgga ttgagcttct taagaaacaa     1980

gatgaagatt ggagaatggg tgatatagtc cacacactga caaacagaag gtggctggaa     2040

aaatgtgtgg tttatgctga aagtcatgac caggccttgg ttggtgataa gacactagca     2100

ttttggttga tggacaagga tatgtatgac ttcatggctt tagacaggcc atctactcct     2160

ctaatagatc gtggtatagc attacacaaa atgattcggc ttattactat gggccttggt     2220

ggtgaagggt atttgaattt tatggggaat gaattcggac atcctgagtg gatcgatttt     2280

ccaaggggtg agcaacatct tcctaatggc aaaatagttc ctgggaataa caacagttat     2340

gataaatgca gacgtagatt tgacttgggt gatgcagatt atctaagata tcatggaatg     2400

caagaatttg atcgagctat gcagcatcta gaagagagat atggtttcat gacttctgag     2460

caccaatata tttcacggaa aaatgaaggt gacagagtta taatctttga aagagacaac     2520

ctggtctttg tcttcaattt tcactggacc aacagttatt cagattacaa ggttggctgt     2580

ttaaagccag ggaaatataa gattgtcttg gattcagatg ataccttgtt tggtggcttc     2640

aataggctca atcatactgc tgagtacttc acctctgaag ggtggtatga tgaccggcct     2700

cgatctttcc tcgtttatgc accttctaga acagcagtgg tgtatgccct tgcggatgga     2760

gttgaatcag agccaattga actgtcagat ggagtagaat cagagccaat tgagctgtca     2820

gttggagttg aatcagagcc aattgagctg tcagttgaag aagctgaatc agagccaatt     2880

gagcggtcag ttgaagaagt tgaatcagag acaactcaac agtcagttga agttgaatca     2940

gagacaactc aacagtcagt tgaagttgaa tcagagacaa ctcaatagtc agttgaagtt     3000

gaaccatgat gacaagtttt tctggaatga atatatcaat ttcataatgg catcagtgca     3060

atcaagtcaa aaccagccaa ccaacttaag gtgattagag caggctctga aactgcaagg     3120

cggagccctt gtgttaactt aatctttagt gcggcagcaa accaacgttg aagtgtttgt     3180

acccattgct tgctaggtca attatgtata gcacgtcaag ttttttgttt ttacttgggg     3240

tttaataggc acaagttgcg tggcgtgtga atgtgaaaca acaaatgtac tgtcctcaga     3300

tcctccgttt ctgtggacat ataaatcctt tttacccata agtgcacaaa ccttaattaa     3360

accctggaaa gttttgcggg aggccttata tatgatttct cctttcagaa ttaaaagttg     3420

agtcatagca atagggccgt gctgatgaaa taattgtttt gtacacggtg aaaatagtat     3480

tgccttacta taaaatataa atggtggaac ctttgcctcc aattgcaaaa aaaaaaaaaa     3540

aaggaattc                                                             3549


<210> 2
<211> 922
<212> PRT
<213> Pisum sativum


<220> 
<223> P. sativum SBE1 amino acid

<400> 2
Met Val Tyr Thr Ile Ser Gly Ile Arg Phe Pro Val Leu Pro Ser Leu 
1               5                   10                  15      
His Lys Ser Thr Leu Arg Cys Asp Arg Arg Ala Ser Ser His Ser Phe 
            20                  25                  30          
Phe Leu Lys Asn Asn Ser Ser Ser Phe Ser Arg Thr Ser Leu Tyr Ala 
        35                  40                  45              
Lys Phe Ser Arg Asp Ser Glu Thr Lys Ser Ser Thr Ile Ala Glu Ser 
    50                  55                  60                  
Asp Lys Val Leu Ile Pro Glu Asp Gln Asp Asn Ser Val Ser Leu Ala 
65                  70                  75                  80  
Asp Gln Leu Glu Asn Pro Asp Ile Thr Ser Glu Asp Ala Gln Asn Leu 
                85                  90                  95      
Glu Asp Leu Thr Met Lys Asp Gly Asn Lys Tyr Asn Ile Asp Glu Ser 
            100                 105                 110         
Thr Ser Ser Tyr Arg Glu Val Gly Asp Glu Lys Gly Ser Val Thr Ser 
        115                 120                 125             
Ser Ser Leu Val Asp Val Asn Thr Asp Thr Gln Ala Lys Lys Thr Ser 
    130                 135                 140                 
Val His Ser Asp Lys Lys Val Lys Val Asp Lys Pro Lys Ile Ile Pro 
145                 150                 155                 160 
Pro Pro Gly Thr Gly Gln Lys Ile Tyr Glu Ile Asp Pro Leu Leu Gln 
                165                 170                 175     
Ala His Arg Gln His Leu Asp Phe Arg Tyr Gly Gln Tyr Lys Arg Ile 
            180                 185                 190         
Arg Glu Glu Ile Asp Lys Tyr Glu Gly Gly Leu Asp Ala Phe Ser Arg 
        195                 200                 205             
Gly Tyr Glu Lys Phe Gly Phe Thr Arg Ser Ala Thr Gly Ile Thr Tyr 
    210                 215                 220                 
Arg Glu Trp Ala Pro Gly Ala Lys Ser Ala Ala Leu Val Gly Asp Phe 
225                 230                 235                 240 
Asn Asn Trp Asn Pro Asn Ala Asp Val Met Thr Lys Asp Ala Phe Gly 
                245                 250                 255     
Val Trp Glu Ile Phe Leu Pro Asn Asn Ala Asp Gly Ser Pro Pro Ile 
            260                 265                 270         
Pro His Gly Ser Arg Val Lys Ile His Met Asp Thr Pro Ser Gly Ile 
        275                 280                 285             
Lys Asp Ser Ile Pro Ala Trp Ile Lys Phe Ser Val Gln Ala Pro Gly 
    290                 295                 300                 
Glu Ile Pro Tyr Asn Gly Ile Tyr Tyr Asp Pro Pro Glu Glu Glu Lys 
305                 310                 315                 320 
Tyr Val Phe Lys His Pro Gln Pro Lys Arg Pro Gln Ser Ile Arg Ile 
                325                 330                 335     
Tyr Glu Ser His Ile Gly Met Ser Ser Pro Glu Pro Lys Ile Asn Thr 
            340                 345                 350         
Tyr Ala Asn Phe Arg Asp Asp Val Leu Pro Arg Ile Lys Lys Leu Gly 
        355                 360                 365             
Tyr Asn Ala Val Gln Ile Met Ala Ile Gln Glu His Ser Tyr Tyr Ala 
    370                 375                 380                 
Ser Phe Gly Tyr His Val Thr Asn Phe Phe Ala Pro Ser Ser Arg Phe 
385                 390                 395                 400 
Gly Thr Pro Glu Asp Leu Lys Ser Leu Ile Asp Arg Ala His Glu Leu 
                405                 410                 415     
Gly Leu Leu Val Leu Met Asp Ile Val His Ser His Ser Ser Asn Asn 
            420                 425                 430         
Thr Leu Asp Gly Leu Asn Met Phe Asp Gly Thr Asp Gly His Tyr Phe 
        435                 440                 445             
His Pro Gly Ser Arg Gly Tyr His Trp Met Trp Asp Ser Arg Leu Phe 
    450                 455                 460                 
Asn Tyr Gly Ser Trp Glu Val Leu Arg Tyr Leu Leu Ser Asn Ala Arg 
465                 470                 475                 480 
Trp Trp Leu Asp Glu Tyr Lys Phe Asp Gly Phe Arg Phe Asp Gly Val 
                485                 490                 495     
Thr Ser Met Met Tyr Thr His His Gly Leu Gln Val Ser Phe Thr Gly 
            500                 505                 510         
Asn Tyr Ser Glu Tyr Phe Gly Leu Ala Thr Asp Val Glu Ala Val Val 
        515                 520                 525             
Tyr Met Met Leu Val Asn Asp Leu Ile His Gly Leu Phe Pro Glu Ala 
    530                 535                 540                 
Val Ser Ile Gly Glu Asp Val Ser Gly Met Pro Thr Phe Cys Leu Pro 
545                 550                 555                 560 
Thr Gln Asp Gly Gly Ile Gly Phe Asn Tyr Arg Leu His Met Ala Val 
                565                 570                 575     
Ala Asp Lys Trp Ile Glu Leu Leu Lys Lys Gln Asp Glu Asp Trp Arg 
            580                 585                 590         
Met Gly Asp Ile Val His Thr Leu Thr Asn Arg Arg Trp Leu Glu Lys 
        595                 600                 605             
Cys Val Val Tyr Ala Glu Ser His Asp Gln Ala Leu Val Gly Asp Lys 
    610                 615                 620                 
Thr Leu Ala Phe Trp Leu Met Asp Lys Asp Met Tyr Asp Phe Met Ala 
625                 630                 635                 640 
Leu Asp Arg Pro Ser Thr Pro Leu Ile Asp Arg Gly Ile Ala Leu His 
                645                 650                 655     
Lys Met Ile Arg Leu Ile Thr Met Gly Leu Gly Gly Glu Gly Tyr Leu 
            660                 665                 670         
Asn Phe Met Gly Asn Glu Phe Gly His Pro Glu Trp Ile Asp Phe Pro 
        675                 680                 685             
Arg Gly Glu Gln His Leu Pro Asn Gly Lys Ile Val Pro Gly Asn Asn 
    690                 695                 700                 
Asn Ser Tyr Asp Lys Cys Arg Arg Arg Phe Asp Leu Gly Asp Ala Asp 
705                 710                 715                 720 
Tyr Leu Arg Tyr His Gly Met Gln Glu Phe Asp Arg Ala Met Gln His 
                725                 730                 735     
Leu Glu Glu Arg Tyr Gly Phe Met Thr Ser Glu His Gln Tyr Ile Ser 
            740                 745                 750         
Arg Lys Asn Glu Gly Asp Arg Val Ile Ile Phe Glu Arg Asp Asn Leu 
        755                 760                 765             
Val Phe Val Phe Asn Phe His Trp Thr Asn Ser Tyr Ser Asp Tyr Lys 
    770                 775                 780                 
Val Gly Cys Leu Lys Pro Gly Lys Tyr Lys Ile Val Leu Asp Ser Asp 
785                 790                 795                 800 
Asp Thr Leu Phe Gly Gly Phe Asn Arg Leu Asn His Thr Ala Glu Tyr 
                805                 810                 815     
Phe Thr Ser Glu Gly Trp Tyr Asp Asp Arg Pro Arg Ser Phe Leu Val 
            820                 825                 830         
Tyr Ala Pro Ser Arg Thr Ala Val Val Tyr Ala Leu Ala Asp Gly Val 
        835                 840                 845             
Glu Ser Glu Pro Ile Glu Leu Ser Asp Gly Val Glu Ser Glu Pro Ile 
    850                 855                 860                 
Glu Leu Ser Val Gly Val Glu Ser Glu Pro Ile Glu Leu Ser Val Glu 
865                 870                 875                 880 
Glu Ala Glu Ser Glu Pro Ile Glu Arg Ser Val Glu Glu Val Glu Ser 
                885                 890                 895     
Glu Thr Thr Gln Gln Ser Val Glu Val Glu Ser Glu Thr Thr Gln Gln 
            900                 905                 910         
Ser Val Glu Val Glu Ser Glu Thr Thr Gln 
        915                 920         

