                         SEQUENCE LISTING

<110>  Alfasigma S.p.A.
 
<120>  NOVEL DNA SEQUENCES ENCODING HUMAN SCFVs ANTIBODIES WITH 
       NEUTRALIZING ACTIVITY AGAINST SARS-CORONAVIRUS-2 AND USES THEREOF

<130>  36596WO

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  740
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76


<220>
<221>  CDS
<222>  (1)..(738)

<400>  1
atg gag gag gtg cag ctg ttg cag tct gca gga ggc ttg gtc cag cct         48
Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

ggc acc ctg gtc acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ttg acg cag tct cca ggc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly           
    130                 135                 140                           

acc ctg tct ttg tct cca ggg gaa aga gcc acc ctc tcc tgc agg gcc        480
Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala           
145                 150                 155                 160           

agt cag agt gtt agc agc agc tac tta gcc tgg tac cag cag aaa cct        528
Ser Gln Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro           
                165                 170                 175               

ggc cag gct ccc agg ctc ctc atc tat ggt gca tcc agc agg gcc act        576
Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr           
            180                 185                 190                   

ggc atc cca gac agg ttc agt ggc agt ggg tct ggg aca gac ttc act        624
Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr           
        195                 200                 205                       

ctc acc atc agc aga ctg gag cct gaa gat ttt gca gtg tat tac tgt        672
Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys           
    210                 215                 220                           

cag caa tat ggt agc tca ccg tac act ttt ggc caa ggg acc aag ctg        720
Gln Gln Tyr Gly Ser Ser Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu           
225                 230                 235                 240           

gag atc aaa cgt gcg gcc gc                                             740
Glu Ile Lys Arg Ala Ala                                                   
                245                                                       


<210>  2
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly 
    130                 135                 140                 


Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Ser Val Ser Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Ser Arg Ala Thr 
            180                 185                 190         


Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Tyr Gly Ser Ser Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Lys Arg Ala Ala 
                245     


<210>  3
<211>  740
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76-58


<220>
<221>  CDS
<222>  (1)..(738)

<400>  3
atg gag gag gtg cag ctg atg cag tct gcg gga ggc ttg gtc cag cct         48
Met Glu Glu Val Gln Leu Met Gln Ser Ala Gly Gly Leu Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac agt tcc aag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ser Ser Lys Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

ggc acc ctg gtc acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ttg acg cag tct ccg gcc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala           
    130                 135                 140                           

acc ctg tct gcg tct cca ggg gaa aga gcc acc ctc tcc tgc agg gcc        480
Thr Leu Ser Ala Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala           
145                 150                 155                 160           

agt cag agt gtt agg agc gac tta gcc tgg tac cag cag aaa cct ggc        528
Ser Gln Ser Val Arg Ser Asp Leu Ala Trp Tyr Gln Gln Lys Pro Gly           
                165                 170                 175               

cgg gct ccc agg ctc ctc atc tat ggt gcg tcc aga agg gcc act ggc        576
Arg Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Arg Arg Ala Thr Gly           
            180                 185                 190                   

atc cca gac agg ttt agt ggc agt ggg tct ggg aca gac ttc act ctc        624
Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu           
        195                 200                 205                       

acc atc agc aga ctg gag cct gaa gat ttt gca gtg tat tac tgt cag        672
Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln           
    210                 215                 220                           

cag tat ggt agc tca cct cgt atc acc ttc ggc caa ggg acc aag gtg        720
Gln Tyr Gly Ser Ser Pro Arg Ile Thr Phe Gly Gln Gly Thr Lys Val           
225                 230                 235                 240           

gaa atc aaa cgt gcg gcc gc                                             740
Glu Ile Lys Arg Ala Ala                                                   
                245                                                       


<210>  4
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Glu Glu Val Gln Leu Met Gln Ser Ala Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ser Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Thr Leu Ser Ala Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Ser Val Arg Ser Asp Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Arg Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Arg Arg Ala Thr Gly 
            180                 185                 190         


Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Tyr Gly Ser Ser Pro Arg Ile Thr Phe Gly Gln Gly Thr Lys Val 
225                 230                 235                 240 


Glu Ile Lys Arg Ala Ala 
                245     


<210>  5
<211>  737
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76-55


<220>
<221>  CDS
<222>  (1)..(735)

<400>  5
atg gag gag gtg cag ctg ttg cag tct gca gga ggc cag gtc cag cct         48
Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Gln Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

gga acc ctg gtc acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ttg acg cag tct cca gcc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala           
    130                 135                 140                           

acc ctg tct gtg tct cca ggg gaa cga gcc acc ctc tcc tgc agg gcc        480
Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala           
145                 150                 155                 160           

agt cag agt gtt agc agc aac tta gcc tgg tac cag cag aaa cct ggc        528
Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro Gly           
                165                 170                 175               

cag gct ccc agg ctc ctc atc tat ggt gca tcc acc agg gcc act ggt        576
Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Thr Arg Ala Thr Gly           
            180                 185                 190                   

atc cca gcc agg ttc agt ggc agt ggg tct ggg aca gag ttc act ctc        624
Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu           
        195                 200                 205                       

acc atc agc agc ctg cag tct gaa gat ttt gca gtg tat tat tgt cag        672
Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys Gln           
    210                 215                 220                           

cag tat gtt agg tca cct ccg act ttt ggc cag ggg acc aag gtg gaa        720
Gln Tyr Val Arg Ser Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu           
225                 230                 235                 240           

atc aaa cgt gcg gcc gc                                                 737
Ile Lys Arg Ala Ala                                                       
                245                                                       


<210>  6
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Gln Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Thr Leu Ser Val Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Ser Val Ser Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Ser Thr Arg Ala Thr Gly 
            180                 185                 190         


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Ser Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Tyr Val Arg Ser Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 


Ile Lys Arg Ala Ala 
                245 


<210>  7
<211>  725
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76-57


<220>
<221>  CDS
<222>  (1)..(723)

<400>  7
atg gag gag gtg cag ctg ttg cag tct gca gga ggc ttg gtc cag cct         48
Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ctc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac aat tcc cag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

gga acc ctg gtc acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ttg acg cag tct ccg ttc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Phe           
    130                 135                 140                           

ctc ctg tct gca tct gtg ggg gac aga gtc agc atc act tgc cgg gcc        480
Leu Leu Ser Ala Ser Val Gly Asp Arg Val Ser Ile Thr Cys Arg Ala           
145                 150                 155                 160           

act cag gac att agc aat aat tta gcc tgg tat caa caa aag tca ggg        528
Thr Gln Asp Ile Ser Asn Asn Leu Ala Trp Tyr Gln Gln Lys Ser Gly           
                165                 170                 175               

aaa gcc cct agc ctc ctg atc tat gct gca tcc act ttg caa agt ggg        576
Lys Ala Pro Ser Leu Leu Ile Tyr Ala Ala Ser Thr Leu Gln Ser Gly           
            180                 185                 190                   

gtc ccg tca agg ttc agc ggc agt aaa tct ggg aca cag ttc act ctc        624
Val Pro Ser Arg Phe Ser Gly Ser Lys Ser Gly Thr Gln Phe Thr Leu           
        195                 200                 205                       

aca atc agc agc ctg caa cct gaa gat ttt gca act tat tac tgt caa        672
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln           
    210                 215                 220                           

cat agg gac att ttt ggc cag ggg acc aag ctg gag atc aaa cgt gcg        720
His Arg Asp Ile Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala           
225                 230                 235                 240           

gcc gc                                                                 725
Ala                                                                       
                                                                          


<210>  8
<211>  241
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Phe 
    130                 135                 140                 


Leu Leu Ser Ala Ser Val Gly Asp Arg Val Ser Ile Thr Cys Arg Ala 
145                 150                 155                 160 


Thr Gln Asp Ile Ser Asn Asn Leu Ala Trp Tyr Gln Gln Lys Ser Gly 
                165                 170                 175     


Lys Ala Pro Ser Leu Leu Ile Tyr Ala Ala Ser Thr Leu Gln Ser Gly 
            180                 185                 190         


Val Pro Ser Arg Phe Ser Gly Ser Lys Ser Gly Thr Gln Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


His Arg Asp Ile Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala 
225                 230                 235                 240 


Ala 
    


<210>  9
<211>  743
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76-77


<220>
<221>  CDS
<222>  (1)..(741)

<400>  9
atg gag gag gtg cag ctg ttg cag tct gca gga ggc ttg gtc cag cct         48
Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

ggc acc ctg gtt acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ctg act cag tct cca ggc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly           
    130                 135                 140                           

acc ctg tct ttg tct cca ggt gag aaa gcc acc ctc ttt tgc agg gcc        480
Thr Leu Ser Leu Ser Pro Gly Glu Lys Ala Thr Leu Phe Cys Arg Ala           
145                 150                 155                 160           

agt cag act ttt agc agc aac tat tta gcc tgg tac cag cag aaa cct        528
Ser Gln Thr Phe Ser Ser Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro           
                165                 170                 175               

ggc cag gct ccc agc ctc ctc atc tat ggt gga tcc acc agg gcc gct        576
Gly Gln Ala Pro Ser Leu Leu Ile Tyr Gly Gly Ser Thr Arg Ala Ala           
            180                 185                 190                   

ggc atc cca gac aga ttc agc ggc agt ggg tct ggg aca gac ttc act        624
Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr           
        195                 200                 205                       

ctc acc atc aac aga ctg gag cct gaa gat ttc gca att tat tac tgt        672
Leu Thr Ile Asn Arg Leu Glu Pro Glu Asp Phe Ala Ile Tyr Tyr Cys           
    210                 215                 220                           

caa gag tat ggt agt tca cct cgg gtc acc ttc ggc caa ggg aca cga        720
Gln Glu Tyr Gly Ser Ser Pro Arg Val Thr Phe Gly Gln Gly Thr Arg           
225                 230                 235                 240           

ctg gag att aaa cgt gcg gcc gc                                         743
Leu Glu Ile Lys Arg Ala Ala                                               
                245                                                       


<210>  10
<211>  247
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Glu Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly 
    130                 135                 140                 


Thr Leu Ser Leu Ser Pro Gly Glu Lys Ala Thr Leu Phe Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Thr Phe Ser Ser Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                165                 170                 175     


Gly Gln Ala Pro Ser Leu Leu Ile Tyr Gly Gly Ser Thr Arg Ala Ala 
            180                 185                 190         


Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
        195                 200                 205             


Leu Thr Ile Asn Arg Leu Glu Pro Glu Asp Phe Ala Ile Tyr Tyr Cys 
    210                 215                 220                 


Gln Glu Tyr Gly Ser Ser Pro Arg Val Thr Phe Gly Gln Gly Thr Arg 
225                 230                 235                 240 


Leu Glu Ile Lys Arg Ala Ala 
                245         


<210>  11
<211>  737
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide and deduced amino acid sequence of scFv 76-46


<220>
<221>  CDS
<222>  (1)..(735)

<400>  11
atg gag gag gtg cag ctg cag cag tct gca gga ggc ttg gtc cag cct         48
Met Glu Glu Val Gln Leu Gln Gln Ser Ala Gly Gly Leu Val Gln Pro           
1               5                   10                  15                

ggg ggg tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt         96
Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser           
            20                  25                  30                    

gcc aac tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag        144
Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
        35                  40                  45                        

tgg gtc tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc        192
Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser           
    50                  55                  60                            

gtg aag ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg        240
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu           
65                  70                  75                  80            

tat ctt caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac        288
Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr           
                85                  90                  95                

tgt gcg aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa        336
Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln           
            100                 105                 110                   

gga acc ctg gtc acc gtc tcc tca ggt ggt ggt ggt agc ggc ggc ggc        384
Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly           
        115                 120                 125                       

ggc tct ggt ggt ggt gga tcc gaa att gtg ctg act cag tct cca gcc        432
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala           
    130                 135                 140                           

acc ctg tct ttg tct cca ggg gaa aga gcc acc ctc tcc tgc agg gcc        480
Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala           
145                 150                 155                 160           

agt gag agt gtt agc agc tat tta gcc tgg tac caa cag aaa cct ggc        528
Ser Glu Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly           
                165                 170                 175               

cag gct ccc agg ctc ctc atc tat ggt gta tcc agc agg gcc act ggc        576
Gln Ala Pro Arg Leu Leu Ile Tyr Gly Val Ser Ser Arg Ala Thr Gly           
            180                 185                 190                   

atc cca gac agg ttc aat ggc agt ggg tct ggg aca gac ttt act ctt        624
Ile Pro Asp Arg Phe Asn Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu           
        195                 200                 205                       

acc atc agc aga ctg gag cct gaa gat ttt gca gtg tat tac tgt cag        672
Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln           
    210                 215                 220                           

cag tat ggt agc tct ccg atc acc ttc ggc caa ggg acc aag gtg gag        720
Gln Tyr Gly Ser Ser Pro Ile Thr Phe Gly Gln Gly Thr Lys Val Glu           
225                 230                 235                 240           

atc aaa cgt gcg gcc gc                                                 737
Ile Lys Arg Ala Ala                                                       
                245                                                       


<210>  12
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Glu Glu Val Gln Leu Gln Gln Ser Ala Gly Gly Leu Val Gln Pro 
1               5                   10                  15      


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser 
            20                  25                  30          


Ala Asn Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Val Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala 
145                 150                 155                 160 


Ser Glu Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Gln Ala Pro Arg Leu Leu Ile Tyr Gly Val Ser Ser Arg Ala Thr Gly 
            180                 185                 190         


Ile Pro Asp Arg Phe Asn Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Tyr Gly Ser Ser Pro Ile Thr Phe Gly Gln Gly Thr Lys Val Glu 
225                 230                 235                 240 


Ile Lys Arg Ala Ala 
                245 


<210>  13
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH1

<400>  13
cctttctatg cggcccagcc ggccatggag caggtgcagc tggtgcagtc tgg              53


<210>  14
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH2

<400>  14
cctttctatg cggcccagcc ggccatggag caggtcaact taagggagtc tgg              53


<210>  15
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH3

<400>  15
cctttctatg cggcccagcc ggccatggag gaggtgcagc tggtggagtc tgg              53


<210>  16
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH4


<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  S = mixture of 50% G and 50% C nucleotides

<400>  16
cctttctatg cggcccagcc ggccatggag caggtgcagc tgcaggagtc sgg              53


<210>  17
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH5

<400>  17
cctttctatg cggcccagcc ggccatggag gaggtgcagc tgttgcagtc tgc              53


<210>  18
<211>  53
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VH6

<400>  18
cctttctatg cggcccagcc ggccatggag caggtacagc tgcagcagtc agg              53


<210>  19
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VHJ1-2-4-5


<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  K = mixture of 50% G and 50% T nucleotides

<400>  19
accagagccg ccgccgccgc taccaccacc acctgaggag acggtgacca gggtkcc          57


<210>  20
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VHJ5

<400>  20
accagagccg ccgccgccgc taccaccacc acctgaagag acggtgacca ttgtccc          57


<210>  21
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VHJ6

<400>  21
accagagccg ccgccgccgc taccaccacc acctgaggag acggtgaccg tggtccc          57


<210>  22
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VK1

<400>  22
agcggcggcg gcggctctgg tggtggtgga tccgacatcc agatgaccca gtctcc           56


<210>  23
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VK2/4


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  Y = mixture of 50% T and 50% C nucleotides

<400>  23
agcggcggcg gcggctctgg tggtggtgga tccgatgttg tgatgacyca gtctcc           56


<210>  24
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VK3

<400>  24
agcggcggcg gcggctctgg tggtggtgga tccgaaattg tgttgacgca gtctcc           56


<210>  25
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VK5

<400>  25
agcggcggcg gcggctctgg tggtggtgga tccgaaacga cactcacgca gtctcc           56


<210>  26
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VK6

<400>  26
agcggcggcg gcggctctgg tggtggtgga tccgaaattg tgctgactca gtctcc           56


<210>  27
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VKJ1

<400>  27
cggcaccggc gcacctgcgg ccgcacgttt gatttccacc ttggtccc                    48


<210>  28
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VKJ2/4

<400>  28
cggcaccggc gcacctgcgg ccgcacgttt gatctccasc ttggtccc                    48


<210>  29
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VKJ3

<400>  29
cggcaccggc gcacctgcgg ccgcacgttt gatatccact ttggtccc                    48


<210>  30
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VKJ5

<400>  30
cggcaccggc gcacctgcgg ccgcacgttt aatctccagt cgtgtccc                    48


<210>  31
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer VL1

<400>  31
agcggcggcg gcggctctgg tggtggtgga tcccagtctg tgttgacgca gccgcc           56


<210>  32
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL2

<400>  32
agcggcggcg gcggctctgg tggtggtgga tcccagtctg ccctgactca gcctgc           56


<210>  33
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL3a

<400>  33
agcggcggcg gcggctctgg tggtggtgga tcctcctatg tgctgactca gccacc           56


<210>  34
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL3b

<400>  34
agcggcggcg gcggctctgg tggtggtgga tcctcctatg tgctgactca gccacc           56


<210>  35
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL4

<400>  35
agcggcggcg gcggctctgg tggtggtgga tcccacgtta tactgactca accgcc           56


<210>  36
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL5

<400>  36
agcggcggcg gcggctctgg tggtggtgga tcccaggctg tgctcactca gccgtc           56


<210>  37
<211>  56
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VL6

<400>  37
agcggcggcg gcggctctgg tggtggtgga tccaatttta tgctgactca gcccca           56


<210>  38
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VLJ1/2/3


<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  S = mixture of 50% G and 50% C nucleotides

<400>  38
cggcaccggc gcacctgcgg ccgcacctag gacggtsacc ttggtccc                    48


<210>  39
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VLJ4/5

<400>  39
cggcaccggc gcacctgcgg ccgcacctaa aacggtgagc tgggtccc                    48


<210>  40
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer KM167

<400>  40
ggctcgtatg ttgtgtggaa ttg                                               23


<210>  41
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer VHJ1-2-4-5


<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  K = mixture of 50% G and 50% T nucleotides

<400>  41
accagagccg ccgccgccgc taccaccacc acctgaggag acggtgacca gggtkcc          57


<210>  42
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer KM167 and VKJ5

<400>  42
cggcaccggc gcacctgcgg ccgcacgttt aatctccagt cgtgtccc                    48


<210>  43
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  43

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
1               5                   10                  15  


<210>  44
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VH CDR3 antibody region

<400>  44

Ala Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile 
1               5                   10          


<210>  45
<211>  351
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Query 1 of scFv 76 / nucleotide and deduced amino acid sequence


<220>
<221>  CDS
<222>  (1)..(351)

<400>  45
gag gtg cag ctg ttg cag tct gca gga ggc ttg gtc cag cct ggg ggg         48
Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro Gly Gly           
1               5                   10                  15                

tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt gcc aac         96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Ala Asn           
            20                  25                  30                    

tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag tgg gtc        144
Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val           
        35                  40                  45                        

tca gtt att tat ccc ggt ggt agc aca ttc tac gca gac tcc gtg aag        192
Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser Val Lys           
    50                  55                  60                            

ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg tat ctt        240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu           
65                  70                  75                  80            

caa atg aac agc ctg aga gtc gag gat acg gcc gtg tat tac tgt gcg        288
Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala           
                85                  90                  95                

aga gat ctt tcg gtg gca ggt gct ttt gat atc tgg ggc caa ggc acc        336
Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln Gly Thr           
            100                 105                 110                   

ctg gtc acc gtc tcc                                                    351
Leu Val Thr Val Ser                                                       
        115                                                               


<210>  46
<211>  117
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  46

Glu Val Gln Leu Leu Gln Ser Ala Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Ala Asn 
            20                  25                  30          


Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Val Ile Tyr Pro Gly Gly Ser Thr Phe Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Leu Ser Val Ala Gly Ala Phe Asp Ile Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser 
        115         


<210>  47
<211>  291
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHV3-53*01 germline analysis sequence


<220>
<221>  CDS
<222>  (1)..(291)

<400>  47
gag gtg cag ctg gtg gag tct gga gga ggc ttg atc cag cct ggg ggg         48
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile Gln Pro Gly Gly           
1               5                   10                  15                

tcc ctg aga ctc tcc tgt gca gcc tct ggg ttc acc gtc agt agc aac         96
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Ser Asn           
            20                  25                  30                    

tac atg agc tgg gtc cgc cag gct cca ggg aag ggg ctg gag tgg gtc        144
Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val           
        35                  40                  45                        

tca gtt att tat agc ggt ggt agc aca tac tac gca gac tcc gtg aag        192
Ser Val Ile Tyr Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys           
    50                  55                  60                            

ggc cga ttc acc atc tcc aga gac aat tcc aag aac acg ctg tat ctt        240
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu           
65                  70                  75                  80            

caa atg aac agc ctg aga gcc gag gac acg gcc gtg tat tac tgt gcg        288
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala           
                85                  90                  95                

aga                                                                    291
Arg                                                                       
                                                                          


<210>  48
<211>  97
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  48

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ile Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Ser Asn 
            20                  25                  30          


Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Val Ile Tyr Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg 
    


<210>  49
<211>  291
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHV3-53*02 germline analysis sequence

<400>  49
gaggtgcagc tggtggagac tggaggaggc ttgatccagc ctggggggtc cctgagactc       60

tcctgtgcag cctctgggtt caccgtcagt agcaactaca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagtt atttatagcg gtggtagcac atactacgca      180

gactccgtga agggccgatt caccatctcc agagacaatt ccaagaacac gctgtatctt      240

caaatgaaca gcctgagagc cgaggacacg gccgtgtatt actgtgcgag a               291


<210>  50
<211>  291
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHV3-53*04 germline analysis sequence

<400>  50
gaggtgcagc tggtggagtc tggaggaggc ttggtccagc ctggggggtc cctgagactc       60

tcctgtgcag cctctgggtt caccgtcagt agcaactaca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagtt atttatagcg gtggtagcac atactacgca      180

gactccgtga agggccgatt caccatctcc agacacaatt ccaagaacac gctgtatctt      240

caaatgaaca gcctgagagc tgaggacacg gccgtgtatt actgtgcgag a               291


<210>  51
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHJ3*02 germline analysis sequence

<400>  51
ggtgcttttg atatctgggg ccaagggaca atggtcaccg tc                          42


<210>  52
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHJ3*01 germline analysis sequence

<400>  52
ggtgcttttg agatctgggg ccaagggaca atggtcaccg tc                          42


<210>  53
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IGHJ1*01 germline analysis sequence

<400>  53
tggggccagg gcaccctggt caccgtctcc                                        30


<210>  54
<211>  197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence variants of the receptor binding domain of 
       the spike protein of the Wuhan SARS-CoV-2 virus strain; sequence 
       corresponds to amino acid positions 332 to 528 of spike RBD


<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Xaa = Lys or Asn

<220>
<221>  VARIANT
<222>  (121)..(121)
<223>  Xaa = Leu or Arg

<220>
<221>  VARIANT
<222>  (122)..(122)
<223>  Xaa = Tyr or Phe

<220>
<221>  VARIANT
<222>  (141)..(141)
<223>  Xaa = Ile or Val

<220>
<221>  VARIANT
<222>  (144)..(144)
<223>  Xaa = Ala or Val

<220>
<221>  VARIANT
<222>  (152)..(152)
<223>  Xaa = Val or Ala

<220>
<221>  VARIANT
<222>  (153)..(153)
<223>  Xaa = Glu or Lys

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Xaa = Asn or Tyr

<220>
<221>  VARIANT
<222>  (189)..(189)
<223>  Xaa = Ala or Ser

<400>  54

Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
1               5                   10                  15      


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
            20                  25                  30          


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
        35                  40                  45              


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
    50                  55                  60                  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
65                  70                  75                  80  


Pro Gly Gln Thr Gly Xaa Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
                85                  90                  95      


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
            100                 105                 110         


Lys Val Gly Gly Asn Tyr Asn Tyr Xaa Xaa Arg Leu Phe Arg Lys Ser 
        115                 120                 125             


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Xaa Tyr Gln Xaa 
    130                 135                 140                 


Gly Ser Thr Pro Cys Asn Gly Xaa Xaa Gly Phe Asn Cys Tyr Phe Pro 
145                 150                 155                 160 


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Xaa Gly Val Gly Tyr Gln Pro 
                165                 170                 175     


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Xaa Pro Ala Thr 
            180                 185                 190         


Val Cys Gly Pro Lys 
        195         


