                         SEQUENCE LISTING

<110>  Berkeley Lights, Inc.
 
<120>  METHODS OF ASSAYING A BIOLOGICAL CELL

<130>  01149-0018-00PCT

<150>  US 63/080,960
<151>  2020-09-21

<150>  US 63/075,269
<151>  2020-09-07

<150>  US 63/211,337
<151>  2021-06-16

<160>  64    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  1
tggtaggctg                                                              10


<210>  2
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  2
gttagctgct                                                              10


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  3
tacataaaga                                                              10


<210>  4
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  4
agccctatca                                                              10


<210>  5
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  5
acctaccgcc                                                              10


<210>  6
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  6
tctccaagac                                                              10


<210>  7
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  7
gtatacatta                                                              10


<210>  8
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  8
agactcgatt                                                              10


<210>  9
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  9
ccaggattaa                                                              10


<210>  10
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  10
ctccttcaag                                                              10


<210>  11
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  11
actacttctg                                                              10


<210>  12
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode

<400>  12
gccttgttgt                                                              10


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  13
cttccgatct tggtaggctg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  14
cttccgatct gttagctgct                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  15
cttccgatct tacataaaga                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  16
cttccgatct agccctatca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  17
cttccgatct acctaccgcc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  18
cttccgatct tctccaagac                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  19
cttccgatct gtatacatta                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  20
cttccgatct agactcgatt                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  21
cttccgatct ccaggattaa                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  22
cttccgatct ctccttcaag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  23
cttccgatct actacttctg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary demultiplexing forward primer

<400>  24
cttccgatct gccttgttgt                                                   20


<210>  25
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  25
ctcacacgac gctcttccga tcttggtagg ctg                                    33


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  26
ctcacacgac gctcttccga tctgttagct gct                                    33


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  27
ctcacacgac gctcttccga tcttacataa aga                                    33


<210>  28
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  28
ctcacacgac gctcttccga tctagcccta tca                                    33


<210>  29
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  29
ctcacacgac gctcttccga tctacctacc gcc                                    33


<210>  30
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  30
ctcacacgac gctcttccga tcttctccaa gac                                    33


<210>  31
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  31
ctcacacgac gctcttccga tctgtataca tta                                    33


<210>  32
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  32
ctcacacgac gctcttccga tctagactcg att                                    33


<210>  33
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  33
ctcacacgac gctcttccga tctccaggat taa                                    33


<210>  34
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  34
ctcacacgac gctcttccga tctctccttc aag                                    33


<210>  35
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  35
ctcacacgac gctcttccga tctactactt ctg                                    33


<210>  36
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode-specific primer

<400>  36
ctcacacgac gctcttccga tctgccttgt tgt                                    33


<210>  37
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  37
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatcttg gtaggctggg       60

g                                                                       61


<210>  38
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  38
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctgt tagctgctgg       60

g                                                                       61


<210>  39
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  39
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctta cataaagagg       60

g                                                                       61


<210>  40
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  40
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctag ccctatcagg       60

g                                                                       61


<210>  41
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  41
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctac ctaccgccgg       60

g                                                                       61


<210>  42
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  42
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatcttc tccaagacgg       60

g                                                                       61


<210>  43
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  43
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctgt atacattagg       60

g                                                                       61


<210>  44
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  44
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctag actcgattgg       60

g                                                                       61


<210>  45
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  45
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctcc aggattaagg       60

g                                                                       61


<210>  46
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  46
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctct ccttcaaggg       60

g                                                                       61


<210>  47
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  47
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctac tacttctggg       60

g                                                                       61


<210>  48
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary first oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 52-Bio

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  n is u

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  2'-O-Me

<220>
<221>  modified_base
<222>  (59)..(61)
<223>  PS linkage

<400>  48
ntatatannn gtggtatcaa cgcagagtac acgacgctct tccgatctgc cttgttgtgg       60

g                                                                       61


<210>  49
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary second oligonucleotide including a capture sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is 5Biosg

<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  n is inosine

<400>  49
naagcagtgg tatcaacgca gagtactttt tttttttttt tttttttttt ttttttvn         58


<210>  50
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary priming sequence

<400>  50
aagcagtggt atcaacgcag agtac                                             25


<210>  51
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary priming sequence

<400>  51
acactctttc cctacacgac gctcttccga tc                                     32


<210>  52
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary priming sequence

<400>  52
aatgatacgg cgaccaccga gatctacact ctttccctac acga                        44


<210>  53
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary priming sequence

<400>  53
caagcagaag acggcatacg agat                                              24


<210>  54
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary heavy chain constant region reverse primer

<400>  54
acagtcactg agctgct                                                      17


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary light chain constant region reverse primer

<400>  55
gactgaggca cctccagatg                                                   20


<210>  56
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Not1 restriction site sequence

<400>  56
gcggccgc                                                                 8


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary barcode specific primer

<400>  57
cttccgatct tggtaggctg                                                   20


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cDNA sequence

<400>  58
acacgacgct cttccgatct tggtaggctg                                        30


<210>  59
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cDNA sequence

<400>  59
cagcctacca agatcggaag agcgtcgtgt                                        30


<210>  60
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary TAP adapter primer

<400>  60
agagtacacg acgctcttcc gatcttggta ggctg                                  35


<210>  61
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cDNA sequence

<400>  61
ctcttccgat cttggtaggc tg                                                22


<210>  62
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cDNA sequence

<400>  62
cagcctacca agatcggaag ag                                                22


<210>  63
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary TAP backbone

<400>  63
tatatatttg tggtatcaac gcagagtaca cgacgctctt ccgatct                     47


<210>  64
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary cDNA sequence

<400>  64
tctcatgtgc tgcgagaagg ctagaaccat ccgac                                  35


