                         SEQUENCE LISTING

<110>  Northeastern University
       D'ONOFRIO, Anthony
       LEWIS, Kim
 
<120>  HYGROMYCIN A COMPOSITIONS AND METHODS OF USE

<130>  21591011PCT

<150>  63/226,357
<151>  2021-07-28

<150>  63/073,073
<151>  2020-09-01

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  1
gcgcatatgt taaaaaaagt ttattatttt ttaatttttt tatttattgt tgc              53


<210>  2
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  2
ggcctcgagt taattttcca tttgcaaaac aaagttatca taagatacct tgtc             54


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  3
acccggaata agcagtcaag                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  4
gctgccttac aagcctctac                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  5
cagggctttc tggtataggg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  6
ggatactttg cgtcgaaggc                                                   20


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  7
tgcatactta gcaccagctt ca                                                22


<210>  8
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  8
gatcgtcgac aagcaaggag gatattttta tgttaaaaaa ag                          42


<210>  9
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  9
gagacctgca ggttaatttt ccatttgcaa aacaaagtta tcataagata cc               52


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  10
atgccatagc atttttatcc                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of the artificial sequence: Primer

<400>  11
agtttatggc gggcgtcctg                                                   20


<210>  12
<211>  341
<212>  PRT
<213>  Borreliella burgdorferi

<400>  12

Met Leu Lys Lys Val Tyr Tyr Phe Leu Ile Phe Leu Phe Ile Val Ala 
1               5                   10                  15      


Cys Ser Ser Ser Asp Asp Gly Lys Ser Glu Ala Lys Thr Val Ser Leu 
            20                  25                  30          


Ile Val Asp Gly Ala Phe Asp Asp Lys Gly Phe Asn Glu Ser Ser Ser 
        35                  40                  45              


Lys Ala Ile Arg Lys Leu Lys Ala Asp Leu Asn Ile Asn Ile Ile Glu 
    50                  55                  60                  


Lys Ala Ser Thr Gly Asn Ser Tyr Leu Gly Asp Ile Ala Asn Leu Glu 
65                  70                  75                  80  


Asp Gly Asn Ser Asn Leu Ile Trp Gly Ile Gly Phe Arg Leu Ser Asp 
                85                  90                  95      


Ile Leu Phe Gln Arg Ala Ser Glu Asn Val Ser Val Asn Tyr Ala Ile 
            100                 105                 110         


Ile Glu Gly Val Tyr Asp Glu Ile Gln Ile Pro Lys Asn Leu Leu Asn 
        115                 120                 125             


Ile Ser Phe Arg Ser Glu Glu Val Ala Phe Leu Ala Gly Tyr Phe Ala 
    130                 135                 140                 


Ser Lys Ala Ser Lys Thr Gly Lys Ile Gly Phe Val Gly Gly Val Arg 
145                 150                 155                 160 


Gly Lys Val Leu Glu Ser Phe Met Tyr Gly Tyr Glu Ala Gly Ala Lys 
                165                 170                 175     


Tyr Ala Asn Ser Asn Ile Lys Val Val Ser Gln Tyr Val Gly Thr Phe 
            180                 185                 190         


Gly Asp Phe Gly Leu Gly Arg Ser Thr Ala Ser Asn Met Tyr Arg Asp 
        195                 200                 205             


Gly Val Asp Ile Ile Phe Ala Ala Ala Gly Leu Ser Gly Ile Gly Val 
    210                 215                 220                 


Ile Glu Ala Ala Lys Glu Leu Gly Pro Asp His Tyr Ile Ile Gly Val 
225                 230                 235                 240 


Asp Gln Asp Gln Ser Tyr Leu Ala Pro Asn Asn Val Ile Val Ser Ala 
                245                 250                 255     


Val Lys Lys Val Asp Ser Leu Met Tyr Ser Leu Thr Lys Lys Tyr Leu 
            260                 265                 270         


Glu Thr Gly Val Leu Asp Gly Gly Lys Thr Met Phe Leu Gly Leu Lys 
        275                 280                 285             


Glu Asp Gly Leu Gly Leu Val Leu Asn Glu Asn Leu Lys Ser Asn Tyr 
    290                 295                 300                 


Ser Glu Ile Tyr Asn Lys Ser Leu Lys Ile Gly Gln Ser Ile Met Asn 
305                 310                 315                 320 


Gly Ile Ile Lys Val Pro Tyr Asp Lys Val Ser Tyr Asp Asn Phe Val 
                325                 330                 335     


Leu Gln Met Glu Asn 
            340     


<210>  13
<211>  383
<212>  PRT
<213>  Borreliella burgdorferi

<400>  13

Met Thr Ile Ser Lys Asn Val Phe Ser Lys Phe Ile Leu Lys Phe Leu 
1               5                   10                  15      


Asn Ser Ser Ala Phe Val Ser Val Phe Ala Leu Phe Val Gly Phe Leu 
            20                  25                  30          


Ile Val Gly Leu Val Val Met Gly Leu Gly His Ser Pro Phe Arg Met 
        35                  40                  45              


Tyr Phe Ile Ile Leu Glu Ile Ile Phe Ser Ser Pro Lys His Leu Gly 
    50                  55                  60                  


Tyr Val Leu Ser Tyr Ser Ala Pro Leu Ile Phe Thr Gly Leu Ser Ile 
65                  70                  75                  80  


Gly Ile Ser Leu Lys Ala Gly Leu Phe Asn Ile Gly Val Glu Gly Gln 
                85                  90                  95      


Phe Ile Leu Gly Ser Ile Val Ala Leu Ile Ala Ser Val Leu Leu Asp 
            100                 105                 110         


Leu Pro Pro Ile Leu His Val Ile Thr Ile Phe Ile Ile Thr Phe Leu 
        115                 120                 125             


Ala Ser Gly Ser Leu Gly Ile Leu Ile Gly Tyr Leu Lys Ala Lys Phe 
    130                 135                 140                 


Asn Ile Ser Glu Val Ile Ser Gly Ile Met Phe Asn Trp Ile Leu Phe 
145                 150                 155                 160 


His Leu Asn Asn Ile Ile Leu Asp Phe Ser Phe Ile Lys Arg Asp Asn 
                165                 170                 175     


Ser Asp Phe Ser Lys Pro Ile Lys Glu Ser Ala Tyr Ile Asp Phe Leu 
            180                 185                 190         


Ala Ser Trp Lys Leu Ser Pro Glu Gly Leu Ala Tyr Arg Ser Ser His 
        195                 200                 205             


Pro Phe Val Asn Glu Leu Leu Lys Ala Pro Leu His Phe Gly Ile Ile 
    210                 215                 220                 


Leu Gly Ile Ile Phe Ala Ile Leu Ile Trp Phe Leu Leu Asn Lys Thr 
225                 230                 235                 240 


Ile Ile Gly Phe Lys Ile Asn Ala Thr Gly Ser Asn Ile Glu Ala Ser 
                245                 250                 255     


Arg Cys Met Gly Ile Asn Val Lys Ala Val Leu Ile Phe Ser Met Phe 
            260                 265                 270         


Leu Ser Ala Ala Val Ala Gly Leu Ala Gly Ala Ile Gln Leu Met Gly 
        275                 280                 285             


Val Asn Lys Ala Ile Phe Lys Leu Ser Tyr Met Gln Gly Ile Gly Phe 
    290                 295                 300                 


Asn Gly Ile Ala Ala Ser Leu Met Gly Asn Asn Ser Pro Ile Gly Ile 
305                 310                 315                 320 


Ile Phe Ser Ser Ile Leu Phe Ser Ile Leu Leu Tyr Gly Ser Ser Arg 
                325                 330                 335     


Val Gln Ser Leu Met Gly Leu Pro Ser Ser Ile Val Ser Leu Met Met 
            340                 345                 350         


Gly Ile Ile Val Leu Val Ile Ser Ala Ser Tyr Phe Leu Asn Lys Ile 
        355                 360                 365             


Val Leu Lys Gly Val Lys Arg Val Lys Tyr Asn Asn Ile Leu Asp 
    370                 375                 380             


<210>  14
<211>  308
<212>  PRT
<213>  Borreliella burgdorferi

<400>  14

Met Ser Asn Ile Ile Ile Phe Leu Ile Ser Glu Thr Leu Ile Asn Ser 
1               5                   10                  15      


Gln Thr Leu Ile Leu Ala Gly Leu Gly Gly Leu Ile Ser Glu Lys Ser 
            20                  25                  30          


Gly Ile Ile Asn Ile Gly Leu Glu Gly Ile Met Thr Ile Gly Ala Phe 
        35                  40                  45              


Ser Gly Ala Thr Val Ala Tyr Phe Thr Asn Asp Pro Leu Phe Ser Ile 
    50                  55                  60                  


Phe Ala Gly Gly Leu Ala Gly Leu Val Leu Ala Ile Leu His Ala Val 
65                  70                  75                  80  


Phe Thr Ile Phe Leu Lys Ser Asp Gln Ile Ile Thr Gly Met Ala Leu 
                85                  90                  95      


Asn Phe Leu Gly Pro Ala Ile Ala Val Phe Ile Ser Thr Leu Ile Phe 
            100                 105                 110         


Ser Ser Ile Ser Thr Pro Pro Ile Glu Ile Lys Leu Pro Ile Leu Phe 
        115                 120                 125             


Asp Gly Ile Leu Asn Lys Thr Ser Phe Ile Phe Gln Ile Phe Gly Lys 
    130                 135                 140                 


Arg Tyr Ser Val Tyr Ile Ala Ile Leu Ser Val Val Leu Phe His Ile 
145                 150                 155                 160 


Val Phe Lys Tyr Thr Lys Ile Gly Leu Arg Ile Asn Ala Ser Gly Glu 
                165                 170                 175     


Asn Pro Glu Val Leu Glu Ser Val Gly Val Ser Val Asn Lys Ile Arg 
            180                 185                 190         


Phe Phe Cys Val Leu Leu Ser Gly Phe Leu Ala Gly Val Ser Gly Ala 
        195                 200                 205             


Val Leu Thr Thr Val Val Ala Ser Ser Tyr Val Gln Gly Val Thr Gly 
    210                 215                 220                 


Gly Gln Gly Phe Ile Ala Ile Val Met Leu Ile Phe Gly Lys Trp Thr 
225                 230                 235                 240 


Pro Leu Gly Val Leu Ile Gly Ser Phe Leu Phe Ser Phe Val Lys Thr 
                245                 250                 255     


Leu Ala Ile Val Leu Ala Gln Leu Pro Phe Phe Ser Leu Ile Met Pro 
            260                 265                 270         


Pro Lys Met Leu Val Ile Thr Pro Tyr Leu Ile Ile Ile Leu Ser Leu 
        275                 280                 285             


Ile Phe Phe Ser Lys Lys Asn Tyr Ala Pro Lys Phe Leu Gly Ile Thr 
    290                 295                 300                 


Tyr Lys Lys His 
305             


<210>  15
<211>  536
<212>  PRT
<213>  Borreliella burgdorferi

<400>  15

Met Lys Glu Asp Val Leu Val Leu Glu Asn Ile Thr Lys Lys Tyr Gly 
1               5                   10                  15      


Asp Phe Val Ala Asn Asp Asn Val Ser Ile Lys Phe Lys Ala Gly Glu 
            20                  25                  30          


Val His Ala Ile Leu Gly Glu Asn Gly Ala Gly Lys Thr Thr Leu Met 
        35                  40                  45              


Lys Thr Ile Tyr Gly Ile His Gln Val Asn Ser Gly Arg Ile Ile Leu 
    50                  55                  60                  


Lys Gly Gln Glu Ile Asn Phe Lys Asp Ser Ser Glu Ala Ile Arg Asn 
65                  70                  75                  80  


Gly Ile Gly Met Val Phe Gln His Phe Met Leu Ile Pro Gln Phe Thr 
                85                  90                  95      


Ala Val Gln Asn Ile Ile Leu Gly Tyr Glu Asn Ser Lys Phe Gly Phe 
            100                 105                 110         


Leu Asp Tyr Lys Gln Ala Arg Lys Lys Ile Ser Ser Leu Ser Glu Lys 
        115                 120                 125             


Tyr Gly Leu Lys Ile Asp Leu Glu Lys Arg Val Glu Asp Leu Ser Val 
    130                 135                 140                 


Gly Met Glu Gln Lys Ile Glu Ile Leu Lys Val Leu Tyr Arg Asn Ala 
145                 150                 155                 160 


Asp Ile Ile Ile Phe Asp Glu Pro Thr Ala Val Leu Ala Pro Ser Glu 
                165                 170                 175     


Val Asp Asp Phe Ile Asn Ile Leu Lys Val Leu Ala Gln Glu Gly His 
            180                 185                 190         


Thr Val Ile Leu Ile Thr His Lys Ile Lys Glu Ile Arg Ser Ile Ala 
        195                 200                 205             


Lys Lys Cys Thr Ile Met Arg Leu Gly Lys Val Val Lys Thr Val Asn 
    210                 215                 220                 


Ile Ala Asp Ile Asp Asp Lys Asp Leu Thr Lys Leu Met Ile Gly Lys 
225                 230                 235                 240 


Glu Val Ala Leu Arg Ser Ser Lys Ile Lys Phe Glu Asn His Phe Asn 
                245                 250                 255     


Ile Leu Glu Ile Lys Asn Leu Ser Val Lys Asp Glu Arg Gly Val Leu 
            260                 265                 270         


Lys Val Lys Asp Val Asn Leu Asp Leu Arg Asn Gly Glu Ile Leu Gly 
        275                 280                 285             


Ile Ser Gly Ile Glu Gly Ser Gly Gln Glu Asp Leu Val Asp Ala Ile 
    290                 295                 300                 


Leu Gly Leu Lys Ser Ile Phe Lys Gly Asp Ile Leu Lys Lys Asn Ser 
305                 310                 315                 320 


Ser Gly Asn Leu Glu Ser Leu Lys Gly Leu Thr Ile Lys Gln Arg Ile 
                325                 330                 335     


Asp Lys Lys Ile Gly Asn Ile Pro Ser Asp Arg Gln Lys His Gly Leu 
            340                 345                 350         


Ile Leu Glu Phe Asn Val Met Gln Asn Ile Gly Leu Lys Ser Phe Asp 
        355                 360                 365             


Asn Pro Asp Tyr Leu Arg Leu Lys Thr Ile His Leu Lys Ser Asn Phe 
    370                 375                 380                 


Asp Leu Lys Phe Asn Phe Phe Asn Phe Ile Lys Arg Gln Phe Asn Lys 
385                 390                 395                 400 


Phe Lys Lys Gln Phe Val Gly Phe Asp Leu Asn Ile Leu Arg Lys Leu 
                405                 410                 415     


Ser Asn Gln Leu Val Ser His Phe Asp Ile Arg Pro Arg Asp Ile Leu 
            420                 425                 430         


Ser Lys Val Lys His Leu Ser Gly Gly Asn Gln Gln Lys Val Ile Val 
        435                 440                 445             


Ala Arg Glu Ile Ser Leu Glu Pro Asp Ile Leu Leu Ala Ile Gln Pro 
    450                 455                 460                 


Thr Arg Gly Leu Asp Val Gly Ala Val Glu Asn Ile Tyr Lys Arg Ile 
465                 470                 475                 480 


Ile Glu Gln Arg Asp Ala Gly Arg Ser Val Leu Leu Val Ser Leu Glu 
                485                 490                 495     


Leu Asp Glu Leu Val Asn Val Cys Asp Arg Ile Ala Val Met His Gly 
            500                 505                 510         


Gly Arg Ile Val Gly Ile Leu Glu Asp Asn Phe Asp Ile Asp Val Ile 
        515                 520                 525             


Gly Lys Met Met Ile Gly Leu Ser 
    530                 535     


