                         SEQUENCE LISTING

<110>  3M Innovative Properties Company
       Erickson, Joshua
       Bhokisham, Narendranath
       Bonilla, Tonya
       Dai, Minghua
 
<120>  Biological Indicator Having Modified Test Microorganism

<130>  82825US002

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  2078
<212>  DNA
<213>  Bacillus stearothermophilus


<220>
<221>  misc_structure
<222>  (1)..(6)
<223>  PstI Restriction Site

<220>
<221>  misc_signal
<222>  (157)..(159)
<223>  Start codon

<220>
<221>  misc_structure
<222>  (157)..(489)
<223>  Coding sequence for truncated sspL gene from Geobacillus 
       stearothermophilus

<220>
<221>  misc_structure
<222>  (490)..(2072)
<223>  Coding sequence for alpha-glucosidase enzyme.

<220>
<221>  misc_signal
<222>  (2070)..(2072)
<223>  Stop codon

<220>
<221>  misc_structure
<222>  (2073)..(2078)
<223>  BbsI Restriction Site

<400>  1
ctgcagaatg gctgcatcaa acttcgcaaa tttaacataa tacatagtac agtcagcagc       60

cattgggcac agccgagaag gcagtgactc cccggattca cggctggtgc aactccagca      120

agcccaacca tactatatat aaaaggagat ataacaatgg ctgcatcaaa cttcgcaaat      180

ttaacataat acatagtaca gtcagcagcc attgggcaca gccgagaagg cagtgactcc      240

ccggattcac ggctggtgca actccagcaa gcccaaccat actatatata aaaggagata      300

taacaatgcc aaaccaatct ggaagtaact cttcaaacca actacttgta cctggcgcag      360

ctcaggtaat cgatcaaatg aaattcgaag gcggcggcgg cagcttgaaa aaaacatggt      420

ggaaagaggg cgttgcgtat caaatttatc ctcgcagctt tatggatgcc aacggcgacg      480

gcatcggtga tcttcgcggc atcatcgaaa agctggatta tttggtggag cttggagtcg      540

acatcgtttg gatttgtccg atttaccggt cgccgaatgc cgataacgga tatgacatca      600

gcgattatta tgccattatg gatgagtttg gaacgatgga tgacttcgat gaattgcttg      660

cccaagccca tcggcgcggg ttgaaaatca ttttggattt ggtcatcaat catacgagtg      720

atgagcatcc gtggtttatc gaatcgcggt catcgcgaga caatccgaag cgcgactggt      780

acatttggcg cgacggcaaa gacgggcgcg aaccgaacaa ctgggaaagc attttcggcg      840

gctcggcatg gcagtatgac gagcggacgg gtcagtatta cttgcatatt tttgacgtca      900

aacagcccga cttgaattgg gaaaacgacg aagtgcggcg ggcgctttat gcgatgatca      960

actggtggct ggataaaggc atcgacggct ttcgcatcga cgcgatttcc cacattaaga     1020

aaaagccggg tcttcccgat ttgccaaatc cgaaggggct gaagtacgtg ccgtcatttg     1080

ctgcgcacat gaaccagcca gggattatgg agtatttgcg agagttgaaa gagcaaacgt     1140

ttgcacgata tgacattatg acggtcggcg aggcgaacgg agtaacggtt gatgaggccg     1200

aacaatgggt cggggaagaa aacggcgtgt ttcatatgat ttttcagttt gagcatttag     1260

ggctttggaa aaggaaagcc gatggttcga tcgatgtccg ccggctgaag cggacgttga     1320

cgaaatggca aaaaggattg gaaaaccgtg ggtggaacgc gctctttttg gaaaaccacg     1380

acttgcctcg atcagtgtcg acatggggaa atgacggcga gtattgggcg gagagcgcga     1440

aggcgcttgg cgcgctctac tttttcatgc aagggacgcc gttcatttac caagggcaag     1500

agatcgggat gacgaacgtg caattctccg acattcgcga ctaccgcgat gtcgctgcct     1560

tgcgtctgta tgaactcgaa cgggcgaacg gccggacaca tgaggaagtg atgaagatca     1620

tttggcaaac cgggcgcgac aactcgcgca ccccgatgca atggtctggt gccccaaacg     1680

ctggattcac gacaggtacg ccatggatca aggtgaacga aaactatcgt acgatcaatg     1740

tcgaggccga gcggcgcgac ccgaactcgg tatggtcgtt ttatcgacaa atgattcagc     1800

ttcggaaagc gaacgagctg tttgtttatg gaacgtacga tctgcttttg gaaaaccacc     1860

catccattta cgcgtacaca agaacgcttg gccgcgatcg ggcgcttatc attgtcaacg     1920

tatccgatcg tccttcactt taccgctatg acggcttccg ccttcagtca agcgatttgg     1980

cgctctcgaa ctacccggtc cgtccgcata aaaatgcgac gcgttttaag ctgaagccgt     2040

acgaggcgcg tgtatacatc tggaaagaat aagaagac                             2078


<210>  2
<211>  2063
<212>  DNA
<213>  Bacillus stearothermophilus


<220>
<221>  misc_structure
<222>  (1)..(6)
<223>  KpnI restriction site

<220>
<221>  misc_structure
<222>  (157)..(159)
<223>  Start codon

<220>
<221>  misc_structure
<222>  (157)..(391)
<223>  Coding sequence for truncated sspL gene

<220>
<221>  misc_structure
<222>  (392)..(2057)
<223>  Coding sequence for alpha-glucosidase gene

<220>
<221>  misc_signal
<222>  (2055)..(2057)
<223>  Stop codon

<220>
<221>  misc_structure
<222>  (2058)..(2063)
<223>  EcoRI restriction site

<400>  2
ggtaccaatg gctgcatcaa acttcgcaaa tttaacataa tacatagtac agtcagcagc       60

cattgggcac agccgagaag gcagtgactc cccggattca cggctggtgc aactccagca      120

agcccaacca tactatatat aaaaggagat ataacaatgg ctgcatcaaa cttcgcaaat      180

ttaacataat acatagtaca gtcagcagcc attgggcaca gccgagaagg cagtgactcc      240

ccggattcac ggctggtgca actccagcaa gcccaaccat actatatata aaaggagata      300

taacaatgcc aaaccaatct ggaagtaact cttcaaacca actacttgta cctggcgcag      360

ctcaggtaat cgatcaaatg aaattcgaaa tgaaaaaaac atggtggaaa gagggcgttg      420

cgtatcaaat ttatcctcgc agctttatgg atgccaacgg cgacggcatc ggtgatcttc      480

gcggcatcat cgaaaagctg gattatttgg tggagcttgg agtcgacatc gtttggattt      540

gtccgattta ccggtcgccg aatgccgata acggatatga catcagcgat tattatgcca      600

ttatggatga gtttggaacg atggatgact tcgatgaatt gcttgcccaa gcccatcggc      660

gcgggttgaa aatcattttg gatttggtca tcaatcatac gagtgatgag catccgtggt      720

ttatcgaatc gcggtcatcg cgagacaatc cgaagcgcga ctggtacatt tggcgcgacg      780

gcaaagacgg gcgcgaaccg aacaactggg aaagcatttt cggcggctcg gcatggcagt      840

atgacgagcg gacgggtcag tattacttgc atatttttga cgtcaaacag cccgacttga      900

attgggaaaa cgacgaagtg cggcgggcgc tttatgcgat gatcaactgg tggctggata      960

aaggcatcga cggctttcgc atcgacgcga tttcccacat taagaaaaag ccgggtcttc     1020

ccgatttgcc aaatccgaag gggctgaagt acgtgccgtc atttgctgcg cacatgaacc     1080

agccagggat tatggagtat ttgcgagagt tgaaagagca aacgtttgca cgatatgaca     1140

ttatgacggt cggcgaggcg aacggagtaa cggttgatga ggccgaacaa tgggtcgggg     1200

aagaaaacgg cgtgtttcat atgatttttc agtttgagca tttagggctt tggaaaagga     1260

aagccgatgg ttcgatcgat gtccgccggc tgaagcggac gttgacgaaa tggcaaaaag     1320

gattggaaaa ccgtgggtgg aacgcgctct ttttggaaaa ccacgacttg cctcgatcag     1380

tgtcgacatg gggaaatgac ggcgagtatt gggcggagag cgcgaaggcg cttggcgcgc     1440

tctacttttt catgcaaggg acgccgttca tttaccaagg gcaagagatc gggatgacga     1500

acgtgcaatt ctccgacatt cgcgactacc gcgatgtcgc tgccttgcgt ctgtatgaac     1560

tcgaacgggc gaacggccgg acacatgagg aagtgatgaa gatcatttgg caaaccgggc     1620

gcgacaactc gcgcaccccg atgcaatggt ctggtgcccc aaacgctgga ttcacgacag     1680

gtacgccatg gatcaaggtg aacgaaaact atcgtacgat caatgtcgag gccgagcggc     1740

gcgacccgaa ctcggtatgg tcgttttatc gacaaatgat tcagcttcgg aaagcgaacg     1800

agctgtttgt ttatggaacg tacgatctgc ttttggaaaa ccacccatcc atttacgcgt     1860

acacaagaac gcttggccgc gatcgggcgc ttatcattgt caacgtatcc gatcgtcctt     1920

cactttaccg ctatgacggc ttccgccttc agtcaagcga tttggcgctc tcgaactacc     1980

cggtccgtcc gcataaaaat gcgacgcgtt ttaagctgaa gccgtacgag gcgcgtgtat     2040

acatctggaa agaataagaa ttc                                             2063


<210>  3
<211>  1137
<212>  DNA
<213>  Bacillus stearothermophilus


<220>
<221>  misc_structure
<222>  (1)..(6)
<223>  HindIII restriction site

<220>
<221>  misc_structure
<222>  (488)..(490)
<223>  Start codon for pyrF gene

<220>
<221>  misc_structure
<222>  (1131)..(1137)
<223>  PstI restriction site

<400>  3
aagcttacgt ggctaatatc acagaaattg cattagcgat cgaggaagcg ggagcggacg       60

gtcttacgat gatcaacaca ctaatcggca tgagactcga tttaaaaacc ggcaaaccga      120

tattagcgaa taaaacaggg ggactttcgg gccctgctgt gaagccggtt gccattcgca      180

tggtgtatga agtcagccag atggtcaaca tcccgattat cggaatggga ggcgtgcaaa      240

cggctgaaga tgccctggaa tttcttctcg cgggagcaag cgcagtcgct gtcggaacag      300

caaactttgt gaatcctttt gcatgtccag agattattga acagctccca tctgttttgc      360

tccaatacgg ctatcaatca attgaagaat gcatcggaag gagctggaat catgaaaaac      420

aacctgccca tcatcgcgct tgattttgcg tcagctgaag aaacacttgc gttcttagcg      480

ccttttcagc aagaaccgtt atttgtaaag gttgggatgg agctttttta tcaagaaggg      540

ccatctatcg tgaaacaact aaaagaaaga aactgcgagc tatttttaga tctaaagctt      600

catgacatcc cgactactgt aaacaaagcg atgaagcgcc ttgccagtct tggagtagac      660

ctcgtcaatg ttcatgctgc cgggggcaaa aaaatgatgc aggcagctct cgaaggctta      720

gaagaaggta cgccggctgg aaaaaaacgt ccgtcactta tcgcggtaac ccagctgaca      780

agcacatctg aacaaatcat gaaagatgaa ctgctgatcg aaaagtctct gattgatacg      840

gttgtgcact acagcaaaca ggcggaagaa agcggactgg atggagtggt ctgctctgtt      900

catgaagcaa aagccattta ccaagcggtg tcgccttcat ttctgactgt cactccgggg      960

atcagaatgt cagaggacgc tgcgaatgac caagttcgcg tagcgacgcc tgccattgca     1020

agagagaaag gttcatcagc gattgtagta ggacgctcga ttacaaaagc ggaagacccg     1080

gtaaaagcct ataaggctgt cagacttgaa tgggagggaa tcaaatcttg aggtacc        1137


