                         SEQUENCE LISTING

<110>  Intervet International B.V.
 
<120>  HA stem vaccine for HA antibody-positive targets

<130>  25059

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Influenza A virus

<400>  1

Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu 
1               5                   10      


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker sequence

<400>  2

Gly Gly Gly Gly 
1               


<210>  3
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 HA stem encoding construct


<220>
<221>  CDS
<222>  (1)..(927)

<400>  3
atg ccc atg ggg tct ctg caa ccg ctg gcc acc ttg tac ctg ctg ggg         48
Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly           
1               5                   10                  15                

atg ctg gtc gct tcc gtg cta gct gac acc att tgt att gga tac cac         96
Met Leu Val Ala Ser Val Leu Ala Asp Thr Ile Cys Ile Gly Tyr His           
            20                  25                  30                    

gct aat aac tct acc gat acc gtc gat acc gtc ctg gaa aag aat gtc        144
Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val           
        35                  40                  45                        

aca gtc acc cac tct gtg aac ctg ctc gaa gac gga gga ggc ggc aag        192
Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Gly Gly Gly Gly Lys           
    50                  55                  60                            

tac gtg tgc agc acc aag ctg agg atg gct acc ggc ctg agg aac aag        240
Tyr Val Cys Ser Thr Lys Leu Arg Met Ala Thr Gly Leu Arg Asn Lys           
65                  70                  75                  80            

ccc tcc aag cag agc cag ggc ctg ttc gga gct atc gct ggc ttc acc        288
Pro Ser Lys Gln Ser Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr           
                85                  90                  95                

gag gga gga tgg acc gga atg atc gac gga tgg tac ggc tac cac cac        336
Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His           
            100                 105                 110                   

cag aac gag cag gga tcc gga tac gct gcc gac cag aag agc acc cag        384
Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln           
        115                 120                 125                       

aac gcc atc gac ggc atc acc aac aag gtg aac tcc gtg atc gag aag        432
Asn Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys           
    130                 135                 140                           

atg aac acc cag tac acc gcc atc ggc tgc gag tac aac aag agc gag        480
Met Asn Thr Gln Tyr Thr Ala Ile Gly Cys Glu Tyr Asn Lys Ser Glu           
145                 150                 155                 160           

aag tgc atg aag cag atc gag gac aag atc gag gag atc gag tcc aaa        528
Lys Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys           
                165                 170                 175               

gtg tgg tgc tac aac gcc gag ctg ctg gtg ctg ctg gag aac gag agg        576
Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg           
            180                 185                 190                   

acc ctg gac ttc cac gac tcc aac gtg aag aac ctg tac gag aag gtg        624
Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val           
        195                 200                 205                       

aag agc cag ctg aag aac aac gcc aag gag atc ggc aac ggc tgc ttc        672
Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe           
    210                 215                 220                           

gag ttc tac cac aag tgc gac aac gag tgc atg gag agc gtg aag aac        720
Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Lys Asn           
225                 230                 235                 240           

ggc acc tac gac tac cca aag tac tcc gag gag agc aag ctg aac cgc        768
Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg           
                245                 250                 255               

gag aag atc gac ggc gtg aag ctc gag tcc atg ggc gtg tac cag atc        816
Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr Gln Ile           
            260                 265                 270                   

ctg gcc atc tac agc acc gtg gcc tcc agc ctg gtg ctg ctg gtg tcc        864
Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser           
        275                 280                 285                       

ctc gga gct att tct ttt tgg atg tgc agc aat ggg tct ctc cag tgt        912
Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys           
    290                 295                 300                           

agg att tgt att tga                                                    927
Arg Ile Cys Ile                                                           
305                                                                       


<210>  4
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Val Leu Ala Asp Thr Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Gly Gly Gly Gly Lys 
    50                  55                  60                  


Tyr Val Cys Ser Thr Lys Leu Arg Met Ala Thr Gly Leu Arg Asn Lys 
65                  70                  75                  80  


Pro Ser Lys Gln Ser Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr 
                85                  90                  95      


Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His 
            100                 105                 110         


Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln 
        115                 120                 125             


Asn Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Glu Lys 
    130                 135                 140                 


Met Asn Thr Gln Tyr Thr Ala Ile Gly Cys Glu Tyr Asn Lys Ser Glu 
145                 150                 155                 160 


Lys Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys 
                165                 170                 175     


Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg 
            180                 185                 190         


Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys Val 
        195                 200                 205             


Lys Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys Phe 
    210                 215                 220                 


Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Lys Asn 
225                 230                 235                 240 


Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ser Lys Leu Asn Arg 
                245                 250                 255     


Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Met Gly Val Tyr Gln Ile 
            260                 265                 270         


Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val Ser 
        275                 280                 285             


Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
    290                 295                 300                 


Arg Ile Cys Ile 
305             


<210>  5
<211>  954
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H3 HA stem encoding construct


<220>
<221>  CDS
<222>  (1)..(954)

<400>  5
atg ccc atg ggc agc ctg cag ccc ctg gcc acc ctg tac ctg ctg ggc         48
Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly           
1               5                   10                  15                

atg ctg gtg gcc agc gtg ctg gcc cag gat ttc ccc ggc aag ggc aac         96
Met Leu Val Ala Ser Val Leu Ala Gln Asp Phe Pro Gly Lys Gly Asn           
            20                  25                  30                    

aac acc gcc acc ctg tgc ctg ggc cac cac gcc gtg ccc aac ggc acc        144
Asn Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr           
        35                  40                  45                        

ctg gtg aag acc atc acc gat gat cag atc gag gtg acc aac gcc acc        192
Leu Val Lys Thr Ile Thr Asp Asp Gln Ile Glu Val Thr Asn Ala Thr           
    50                  55                  60                            

gag ctg gtg cag aac ggc ggc ggc ggc cac tac atc tgc cag aac acc        240
Glu Leu Val Gln Asn Gly Gly Gly Gly His Tyr Ile Cys Gln Asn Thr           
65                  70                  75                  80            

ctg aag ctg gcc acc ggc atg aga aac aag ccc gag aag cag acc cag        288
Leu Lys Leu Ala Thr Gly Met Arg Asn Lys Pro Glu Lys Gln Thr Gln           
                85                  90                  95                

ggc atc ttc ggc gcc atc gcc ggc ttc acc gag aac ggc tgg gag ggc        336
Gly Ile Phe Gly Ala Ile Ala Gly Phe Thr Glu Asn Gly Trp Glu Gly           
            100                 105                 110                   

atg gtg aac ggc tgg tac ggc ttc aga cac cag aac agc gag ggc atc        384
Met Val Asn Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly Ile           
        115                 120                 125                       

ggc cag gcc gcc gat ctg aag agc acc cag gcc gcc atc aac cag atc        432
Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asn Gln Ile           
    130                 135                 140                           

aac ggc aag ctg aac aga gtg atc gag aag acc aac gag aag tac cac        480
Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Tyr His           
145                 150                 155                 160           

cag atc gag tgc gag tac aac gag agc gag ggc tgc atg aag cag atc        528
Gln Ile Glu Cys Glu Tyr Asn Glu Ser Glu Gly Cys Met Lys Gln Ile           
                165                 170                 175               

gag gat aag atc gag gag atc gag agc aag ctg tgg agc tac aac gcc        576
Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys Leu Trp Ser Tyr Asn Ala           
            180                 185                 190                   

gag ctg ctg gtg gcc ctg gag aac cag cac acc atc gat ctg acc gat        624
Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr Asp           
        195                 200                 205                       

agc gag atg aac aag ctg ttc gag aag acc aga aag cag ctg aga gag        672
Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Lys Gln Leu Arg Glu           
    210                 215                 220                           

aac gcc gag gat atg ggc aac ggc tgc ttc aag atc tac cac aag tgc        720
Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys Cys           
225                 230                 235                 240           

gat aac agc tgc atg gag agc atc aga aac ggc acc tac gat cac aac        768
Asp Asn Ser Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asp His Asn           
                245                 250                 255               

gag tac aga gat gag gcc gtg aac aac aga ttc cag atc aag agc gtg        816
Glu Tyr Arg Asp Glu Ala Val Asn Asn Arg Phe Gln Ile Lys Ser Val           
            260                 265                 270                   

ctg gag aag agc ggc tac aag gat tgg atc ctg tgg atc agc ttc gcc        864
Leu Glu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe Ala           
        275                 280                 285                       

atc agc tgc ttc ctg ctg tgc gcc atc tgg atg ggc ttc atc atc tgg        912
Ile Ser Cys Phe Leu Leu Cys Ala Ile Trp Met Gly Phe Ile Ile Trp           
    290                 295                 300                           

gcc tgc cag aag ggc aac atc aga tgc aac atc tgc atc tga                954
Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile                       
305                 310                 315                               


<210>  6
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Val Leu Ala Gln Asp Phe Pro Gly Lys Gly Asn 
            20                  25                  30          


Asn Thr Ala Thr Leu Cys Leu Gly His His Ala Val Pro Asn Gly Thr 
        35                  40                  45              


Leu Val Lys Thr Ile Thr Asp Asp Gln Ile Glu Val Thr Asn Ala Thr 
    50                  55                  60                  


Glu Leu Val Gln Asn Gly Gly Gly Gly His Tyr Ile Cys Gln Asn Thr 
65                  70                  75                  80  


Leu Lys Leu Ala Thr Gly Met Arg Asn Lys Pro Glu Lys Gln Thr Gln 
                85                  90                  95      


Gly Ile Phe Gly Ala Ile Ala Gly Phe Thr Glu Asn Gly Trp Glu Gly 
            100                 105                 110         


Met Val Asn Gly Trp Tyr Gly Phe Arg His Gln Asn Ser Glu Gly Ile 
        115                 120                 125             


Gly Gln Ala Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asn Gln Ile 
    130                 135                 140                 


Asn Gly Lys Leu Asn Arg Val Ile Glu Lys Thr Asn Glu Lys Tyr His 
145                 150                 155                 160 


Gln Ile Glu Cys Glu Tyr Asn Glu Ser Glu Gly Cys Met Lys Gln Ile 
                165                 170                 175     


Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys Leu Trp Ser Tyr Asn Ala 
            180                 185                 190         


Glu Leu Leu Val Ala Leu Glu Asn Gln His Thr Ile Asp Leu Thr Asp 
        195                 200                 205             


Ser Glu Met Asn Lys Leu Phe Glu Lys Thr Arg Lys Gln Leu Arg Glu 
    210                 215                 220                 


Asn Ala Glu Asp Met Gly Asn Gly Cys Phe Lys Ile Tyr His Lys Cys 
225                 230                 235                 240 


Asp Asn Ser Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asp His Asn 
                245                 250                 255     


Glu Tyr Arg Asp Glu Ala Val Asn Asn Arg Phe Gln Ile Lys Ser Val 
            260                 265                 270         


Leu Glu Lys Ser Gly Tyr Lys Asp Trp Ile Leu Trp Ile Ser Phe Ala 
        275                 280                 285             


Ile Ser Cys Phe Leu Leu Cys Ala Ile Trp Met Gly Phe Ile Ile Trp 
    290                 295                 300                 


Ala Cys Gln Lys Gly Asn Ile Arg Cys Asn Ile Cys Ile 
305                 310                 315         


<210>  7
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H5 HA stem encoding construct


<220>
<221>  CDS
<222>  (1)..(927)

<400>  7
atg ccc atg ggg tct ctg caa ccg ctg gcc acc ttg tac ctg ctg ggg         48
Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly           
1               5                   10                  15                

atg ctg gtc gct tcc gtg cta gca gac cag att tgt atc ggc tat cat         96
Met Leu Val Ala Ser Val Leu Ala Asp Gln Ile Cys Ile Gly Tyr His           
            20                  25                  30                    

gcc aac aac tca aca gaa cag gtg gac act att atg gag aag aat gtg        144
Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val           
        35                  40                  45                        

aca gtg acc cac gca cag gac atc ctg gag aag gga gga ggc ggc aag        192
Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Gly Gly Gly Gly Lys           
    50                  55                  60                            

tac gtg tgc tct aac cgg ctg gtg ctg gcc acc ggc ctg aga aat aag        240
Tyr Val Cys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Lys           
65                  70                  75                  80            

ccc cag aag gag aca cag ggc ctg ttc gga gca atc gca ggc ttt acc        288
Pro Gln Lys Glu Thr Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr           
                85                  90                  95                

gag gga gga tgg cag gga atg gtg gat gga tgg tac ggc tat cac cac        336
Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His           
            100                 105                 110                   

tct aac gag cag ggc agc ggc tat gcc gcc gac aag gag tct acc cag        384
Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln           
        115                 120                 125                       

aag gcc atc gat ggc gtg aca aac aag gtg aat agc atc atc gac aag        432
Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys           
    130                 135                 140                           

atg aat acc cag tac gag gcc atc ggc tgc gag tat aac aat tcc gag        480
Met Asn Thr Gln Tyr Glu Ala Ile Gly Cys Glu Tyr Asn Asn Ser Glu           
145                 150                 155                 160           

agg tgt atg aag cag atc gag gat aag atc gag gag atc gag tct aaa        528
Arg Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys           
                165                 170                 175               

gtg tgg tgc tac aac gcc gag ctg ctg gtg ctg atg gag aat gag cgc        576
Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg           
            180                 185                 190                   

aca ctg gac ttc cac gat agc aac gtg aag aat ctg tat gac aag gtg        624
Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val           
        195                 200                 205                       

agg ctg cag ctg cgc gat aac gcc aag gag ctg ggc aat ggc tgt ttc        672
Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe           
    210                 215                 220                           

gag ttt tac cac aag tgc gac aac gag tgt atg gag agc gtg cgg aat        720
Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn           
225                 230                 235                 240           

ggc acc tac gat tat cct cag tat tcc gag gag gcc cgg ctg aag aga        768
Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg           
                245                 250                 255               

gag gag atc tcc ggc gtg aag ctg gag tct atc ggc atc tac cag atc        816
Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Ile Tyr Gln Ile           
            260                 265                 270                   

ctg tcc atc tat tcc aca gtg gcc agc tcc ctg gcc ctg gcc atc atg        864
Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met           
        275                 280                 285                       

gtg gct ggg ctg tcc ctg tgg atg tgc tct aac ggg tcc ctg cag tgt        912
Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys           
    290                 295                 300                           

cgc att tgt att tga                                                    927
Arg Ile Cys Ile                                                           
305                                                                       


<210>  8
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Val Leu Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Gly Gly Gly Gly Lys 
    50                  55                  60                  


Tyr Val Cys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Lys 
65                  70                  75                  80  


Pro Gln Lys Glu Thr Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr 
                85                  90                  95      


Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His 
            100                 105                 110         


Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln 
        115                 120                 125             


Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys 
    130                 135                 140                 


Met Asn Thr Gln Tyr Glu Ala Ile Gly Cys Glu Tyr Asn Asn Ser Glu 
145                 150                 155                 160 


Arg Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys 
                165                 170                 175     


Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg 
            180                 185                 190         


Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val 
        195                 200                 205             


Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe 
    210                 215                 220                 


Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn 
225                 230                 235                 240 


Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg 
                245                 250                 255     


Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Ile Tyr Gln Ile 
            260                 265                 270         


Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met 
        275                 280                 285             


Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
    290                 295                 300                 


Arg Ile Cys Ile 
305             


<210>  9
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H7 HA stem encoding construct


<220>
<221>  CDS
<222>  (1)..(927)

<400>  9
atg ccc atg ggg tct ctg caa ccg ctg gcc acc ttg tac ctg ctg ggg         48
Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly           
1               5                   10                  15                

atg ctg gtc gct tcc gtg cta gca gac aaa atc tgc ctg gga cat cat         96
Met Leu Val Ala Ser Val Leu Ala Asp Lys Ile Cys Leu Gly His His           
            20                  25                  30                    

gcc gtg agc aat gga acc aaa gtg aat acc ctg acc gaa aga gga gtg        144
Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val           
        35                  40                  45                        

gaa gtc gtg aac gca act gag aca gtg gag agg gga gga ggc ggc cgg        192
Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Gly Gly Gly Gly Arg           
    50                  55                  60                            

tac gtg tgc cag cgg agc ctg ctg ctg gca aca ggc atg aag aac aag        240
Tyr Val Cys Gln Arg Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Lys           
65                  70                  75                  80            

ccc gag aag cct aag gga cag ggc ctg ttc gga gca atc gca ggc ttt        288
Pro Glu Lys Pro Lys Gly Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe           
                85                  90                  95                

acc gag aac ggc tgg gag ggc ctg atc gat ggc tgg tac ggc ttc cgg        336
Thr Glu Asn Gly Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg           
            100                 105                 110                   

cac cag aat gca cag gga gag ggc acc gcc gcc gat tat aag agc aca        384
His Gln Asn Ala Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr           
        115                 120                 125                       

cag tcc gcc atc gac cag atc acc ggc aag ctg aac cgg ctg atc gag        432
Gln Ser Ala Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu           
    130                 135                 140                           

aag aca aat cag cag tac gag ctg atc gat tgc gag tat aac gag agc        480
Lys Thr Asn Gln Gln Tyr Glu Leu Ile Asp Cys Glu Tyr Asn Glu Ser           
145                 150                 155                 160           

gag aag tgt atg aag cag atc gag gac aag atc gag gag atc gag tcc        528
Glu Lys Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser           
                165                 170                 175               

aaa gtg tgg tgc tac aac gcc gag ctg ctg gtg gct atg gag aat cag        576
Lys Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln           
            180                 185                 190                   

cac aca atc gat ctg gcc gac tcc gag atg gat aag ctg tat gag cgg        624
His Thr Ile Asp Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg           
        195                 200                 205                       

gtg aag aga cag ctg agg gag aat gcc gag gag gac ggc acc ggc tgt        672
Val Lys Arg Gln Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys           
    210                 215                 220                           

ttc gag att ttc cac aag tgc gac gat gac tgt atg gcc tcc atc aga        720
Phe Glu Ile Phe His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg           
225                 230                 235                 240           

aac aat aca tac gac cac tct aag tat agg gag gag gcc atg cag aac        768
Asn Asn Thr Tyr Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn           
                245                 250                 255               

cgc atc cag atc gat ccc gtg aag tta att aac ggc tat aag gac gtg        816
Arg Ile Gln Ile Asp Pro Val Lys Leu Ile Asn Gly Tyr Lys Asp Val           
            260                 265                 270                   

atc ctg tgg ttc tct ttt ggc gcc agc tgt ttt atc ctg ctg gcc atc        864
Ile Leu Trp Phe Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile           
        275                 280                 285                       

gtg atg gga ctg gtg ttt att tgc gtc aag aac ggg aac atg agg tgt        912
Val Met Gly Leu Val Phe Ile Cys Val Lys Asn Gly Asn Met Arg Cys           
    290                 295                 300                           

act att tgt att tga                                                    927
Thr Ile Cys Ile                                                           
305                                                                       


<210>  10
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Val Leu Ala Asp Lys Ile Cys Leu Gly His His 
            20                  25                  30          


Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val 
        35                  40                  45              


Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Gly Gly Gly Gly Arg 
    50                  55                  60                  


Tyr Val Cys Gln Arg Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Lys 
65                  70                  75                  80  


Pro Glu Lys Pro Lys Gly Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                85                  90                  95      


Thr Glu Asn Gly Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg 
            100                 105                 110         


His Gln Asn Ala Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr 
        115                 120                 125             


Gln Ser Ala Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu 
    130                 135                 140                 


Lys Thr Asn Gln Gln Tyr Glu Leu Ile Asp Cys Glu Tyr Asn Glu Ser 
145                 150                 155                 160 


Glu Lys Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser 
                165                 170                 175     


Lys Val Trp Cys Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln 
            180                 185                 190         


His Thr Ile Asp Leu Ala Asp Ser Glu Met Asp Lys Leu Tyr Glu Arg 
        195                 200                 205             


Val Lys Arg Gln Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys 
    210                 215                 220                 


Phe Glu Ile Phe His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg 
225                 230                 235                 240 


Asn Asn Thr Tyr Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn 
                245                 250                 255     


Arg Ile Gln Ile Asp Pro Val Lys Leu Ile Asn Gly Tyr Lys Asp Val 
            260                 265                 270         


Ile Leu Trp Phe Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile 
        275                 280                 285             


Val Met Gly Leu Val Phe Ile Cys Val Lys Asn Gly Asn Met Arg Cys 
    290                 295                 300                 


Thr Ile Cys Ile 
305             


<210>  11
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H9 HA stem encoding construct


<220>
<221>  CDS
<222>  (1)..(927)

<400>  11
atg ccc atg ggg tct ctg caa ccg ctg gcc acc ttg tac ctg ctg ggg         48
Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly           
1               5                   10                  15                

atg ctg gtc gct tcc gtg cta gca gat aaa atc tgt att gga cat cag         96
Met Leu Val Ala Ser Val Leu Ala Asp Lys Ile Cys Ile Gly His Gln           
            20                  25                  30                    

tca act aac tct acc gaa acc gtg gac act ctg acc gaa act aac gtg        144
Ser Thr Asn Ser Thr Glu Thr Val Asp Thr Leu Thr Glu Thr Asn Val           
        35                  40                  45                        

ccc gtg aca cat gcc aaa gag ctg ctg cac act ggc gga ggg ggc aag        192
Pro Val Thr His Ala Lys Glu Leu Leu His Thr Gly Gly Gly Gly Lys           
    50                  55                  60                            

tac atc tgc gtg aag agc ctg aaa ctg gca att ggg ctg cgc aac aag        240
Tyr Ile Cys Val Lys Ser Leu Lys Leu Ala Ile Gly Leu Arg Asn Lys           
65                  70                  75                  80            

ccc gcc aaa agc tcc cag gga ctg ttc ggg gca atc gcc gga ttt act        288
Pro Ala Lys Ser Ser Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr           
                85                  90                  95                

gag gga ggg tgg cct ggg ctg gtg gca ggc tgg tac gga ttc cag cac        336
Glu Gly Gly Trp Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His           
            100                 105                 110                   

tcc aac gat cag ggc gtg gga atg gcc gct gat agg gac agc aca cag        384
Ser Asn Asp Gln Gly Val Gly Met Ala Ala Asp Arg Asp Ser Thr Gln           
        115                 120                 125                       

aag gct gtg gac aaa atc act tcc aag gtg aac aat att gtg gat aag        432
Lys Ala Val Asp Lys Ile Thr Ser Lys Val Asn Asn Ile Val Asp Lys           
    130                 135                 140                           

atg aac aaa cag tac gag atc att gac tgc gaa tac aat gag agc gaa        480
Met Asn Lys Gln Tyr Glu Ile Ile Asp Cys Glu Tyr Asn Glu Ser Glu           
145                 150                 155                 160           

acc tgc atg aaa cag atc gag gat aag atc gag gaa atc gaa tcc aag        528
Thr Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys           
                165                 170                 175               

atc tgg tgc tac aac gct gag ctg ctg gtg ctg ctg gaa aat cag aag        576
Ile Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Gln Lys           
            180                 185                 190                   

aca ctg gat gag cac gac gca aac gtg aac aat ctg tac aat aag gtg        624
Thr Leu Asp Glu His Asp Ala Asn Val Asn Asn Leu Tyr Asn Lys Val           
        195                 200                 205                       

aaa aga gct ctg ggc agc aac gca atg gag gac ggg aaa ggc tgc ttt        672
Lys Arg Ala Leu Gly Ser Asn Ala Met Glu Asp Gly Lys Gly Cys Phe           
    210                 215                 220                           

gaa ctg tac cac aag tgc gat gac cag tgc atg gaa act atc agg aac        720
Glu Leu Tyr His Lys Cys Asp Asp Gln Cys Met Glu Thr Ile Arg Asn           
225                 230                 235                 240           

gga acc tac aat aga agg aaa tac cag gaa gag tcc cgc ctg gag cgg        768
Gly Thr Tyr Asn Arg Arg Lys Tyr Gln Glu Glu Ser Arg Leu Glu Arg           
                245                 250                 255               

cag aaa att gaa ggg gtg aag ctc gag agc gaa ggc acc tac aag atc        816
Gln Lys Ile Glu Gly Val Lys Leu Glu Ser Glu Gly Thr Tyr Lys Ile           
            260                 265                 270                   

ctg acc att tac tcc aca gtg gcc agc tcc ctg gtg ctg gct atg gga        864
Leu Thr Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Ala Met Gly           
        275                 280                 285                       

ttc gct gcc ttc ctg ttt tgg gct atg agc aat ggg agt tgt agg tgt        912
Phe Ala Ala Phe Leu Phe Trp Ala Met Ser Asn Gly Ser Cys Arg Cys           
    290                 295                 300                           

aac atc tgt atc tga                                                    927
Asn Ile Cys Ile                                                           
305                                                                       


<210>  12
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu Tyr Leu Leu Gly 
1               5                   10                  15      


Met Leu Val Ala Ser Val Leu Ala Asp Lys Ile Cys Ile Gly His Gln 
            20                  25                  30          


Ser Thr Asn Ser Thr Glu Thr Val Asp Thr Leu Thr Glu Thr Asn Val 
        35                  40                  45              


Pro Val Thr His Ala Lys Glu Leu Leu His Thr Gly Gly Gly Gly Lys 
    50                  55                  60                  


Tyr Ile Cys Val Lys Ser Leu Lys Leu Ala Ile Gly Leu Arg Asn Lys 
65                  70                  75                  80  


Pro Ala Lys Ser Ser Gln Gly Leu Phe Gly Ala Ile Ala Gly Phe Thr 
                85                  90                  95      


Glu Gly Gly Trp Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His 
            100                 105                 110         


Ser Asn Asp Gln Gly Val Gly Met Ala Ala Asp Arg Asp Ser Thr Gln 
        115                 120                 125             


Lys Ala Val Asp Lys Ile Thr Ser Lys Val Asn Asn Ile Val Asp Lys 
    130                 135                 140                 


Met Asn Lys Gln Tyr Glu Ile Ile Asp Cys Glu Tyr Asn Glu Ser Glu 
145                 150                 155                 160 


Thr Cys Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Glu Ser Lys 
                165                 170                 175     


Ile Trp Cys Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Gln Lys 
            180                 185                 190         


Thr Leu Asp Glu His Asp Ala Asn Val Asn Asn Leu Tyr Asn Lys Val 
        195                 200                 205             


Lys Arg Ala Leu Gly Ser Asn Ala Met Glu Asp Gly Lys Gly Cys Phe 
    210                 215                 220                 


Glu Leu Tyr His Lys Cys Asp Asp Gln Cys Met Glu Thr Ile Arg Asn 
225                 230                 235                 240 


Gly Thr Tyr Asn Arg Arg Lys Tyr Gln Glu Glu Ser Arg Leu Glu Arg 
                245                 250                 255     


Gln Lys Ile Glu Gly Val Lys Leu Glu Ser Glu Gly Thr Tyr Lys Ile 
            260                 265                 270         


Leu Thr Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Ala Met Gly 
        275                 280                 285             


Phe Ala Ala Phe Leu Phe Trp Ala Met Ser Asn Gly Ser Cys Arg Cys 
    290                 295                 300                 


Asn Ile Cys Ile 
305             


<210>  13
<211>  10705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA plasmid for expression of VEEV replicon RNA

<400>  13
gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta       60

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      120

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      180

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      240

ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc      300

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      360

atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      420

gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag      480

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      540

aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga      600

ggtctatata agcagagctc tctggctaac tagtaatacg actcactata gcgccgccta      660

tctgatgagt ccgtgaggac gaaactatag gaaaggaatt cctatagtca taggcggcgc      720

atgagagaag cccagaccaa ttacctaccc aaaatggaga aagttcacgt tgacatcgag      780

gaagacagcc cattcctcag agctttgcag cggagcttcc cgcagtttga ggtagaagcc      840

aagcaggtca ctgataatga ccatgctaat gccagagcgt tttcgcatct ggcttcaaaa      900

ctgatcgaaa cggaggtgga cccatccgac acgatccttg acattggaag tgcgcccgcc      960

cgcagaatgt attctaagca caagtatcat tgtatctgtc cgatgagatg tgcggaagat     1020

ccggacagat tgtataagta tgcaactaag ctgaagaaaa actgtaagga aataactgat     1080

aaggaattgg acaagaaaat gaaggaactc gccgccgtca tgagcgaccc tgacctggaa     1140

actgagacta tgtgcctcca cgacgacgag tcgtgtcgct acgaagggca agtcgctgtt     1200

taccaggatg tatacgcggt tgacggaccg acaagtctct atcaccaagc caataaggga     1260

gttagagtcg cctactggat aggctttgac accacccctt ttatgtttaa gaacttggct     1320

ggagcatatc catcatactc taccaactgg gccgacgaaa ccgtgttaac ggctcgtaac     1380

ataggcctat gcagctctga cgttatggag cggtcacgta gagggatgtc cattcttaga     1440

aagaagtatt tgaaaccatc caacaatgtt ctattctctg ttggctcgac catctaccac     1500

gagaagaggg acttactgag gagctggcac ctgccgtctg tatttcactt acgtggcaag     1560

caaaattaca cctgccgctg cgagaccatc gtgtcctgcg acggctacgt ggtgaagcgc     1620

atcgccatct cccccggcct gtacggcaag ccctccggct acgccgccac catgcaccgc     1680

gagggcttcc tgtgctgcaa ggtgacagac accctgaacg gcgagcgcgt gtccttcccc     1740

gtgtgcacct acgtgcccgc caccctgtgc gaccagatga ccggcatcct ggccaccgac     1800

gtgtccgccg acgacgccca gaagctgctg gtgggcctga accagcgcat cgtggtgaac     1860

ggtcgcaccc agagaaacac caataccatg aaaaattacc ttttgcccgt agtggcccag     1920

gcatttgcta ggtgggcaaa ggaatataag gaagatcaag aagatgaaag gccactagga     1980

ctacgagata gacagttagt catggggtgt tgttgggctt ttagaaggca caagataaca     2040

tctatttata agcgcccgga tacccaaacc atcatcaaag tgaacagcga tttccactca     2100

ttcgtgctgc ccaggatagg cagtaacaca ttggagatcg ggctgagaac aagaatcagg     2160

aaaatgttag aggagcacaa ggagccgtca cctctcatta ccgccgagga cgtacaagaa     2220

gctaagtgcg cagccgatga ggctaaggag gtgcgtgaag ccgaggagtt gcgcgcagct     2280

ctaccacctt tggcagctga tgttgaggag cccactctgg aggcagacgt cgacttgatg     2340

ttacaagagg ctggggccgg ctcagtggag acacctcgtg gcttgataaa ggttaccagc     2400

tacgatggcg aggacaagat cggctcttac gctgtgcttt ctccgcaggc tgtactcaag     2460

agtgaaaaat tatcttgcat ccaccctctc gctgaacaag tcatagtgat aacacactct     2520

ggccgaaaag ggcgttatgc cgtggaacca taccatggta aagtagtggt gccagaggga     2580

catgcaatac ccgtccagga ctttcaagct ctgagtgaaa gtgccaccat tgtgtacaac     2640

gaacgtgagt tcgtaaacag gtacctgcac catattgcca cacatggagg agcgctgaac     2700

actgatgaag aatattacaa aactgtcaag cccagcgagc acgacggcga atacctgtac     2760

gacatcgaca ggaaacagtg cgtcaagaaa gaactagtca ctgggctagg gctcacaggc     2820

gagctggtgg atcctccctt ccatgaattc gcctacgaga gtctgagaac acgaccagcc     2880

gctccttacc aagtaccaac cataggggtg tatggcgtgc caggatcagg caagtctggc     2940

atcattaaaa gcgcagtcac caaaaaagat ctagtggtga gcgccaagaa agaaaactgt     3000

gcagaaatta taagggacgt caagaaaatg aaagggctgg acgtcaatgc cagaactgtg     3060

gactcagtgc tcttgaatgg atgcaaacac cccgtagaga ccctgtatat tgacgaagct     3120

tttgcttgtc atgcaggtac tctcagagcg ctcatagcca ttataagacc taaaaaggca     3180

gtgctctgcg gggatcccaa acagtgcggt ttttttaaca tgatgtgcct gaaagtgcat     3240

tttaaccacg agatttgcac acaagtcttc cacaaaagca tctctcgccg ttgcactaaa     3300

tctgtgactt cggtcgtctc aaccttgttt tacgacaaaa aaatgagaac gacgaatccg     3360

aaagagacta agattgtgat tgacactacc ggcagtacca aacctaagca ggacgatctc     3420

attctcactt gtttcagagg gtgggtgaag cagttgcaaa tagattacaa aggcaacgaa     3480

ataatgacgg cagctgcctc tcaagggctg acccgtaaag gtgtgtatgc cgttcggtac     3540

aaggtgaatg aaaatcctct gtacgcaccc acctcagaac atgtgaacgt cctactgacc     3600

cgcacggagg accgcatcgt gtggaaaaca ctagccggcg acccatggat aaaaacactg     3660

actgccaagt accctgggaa tttcactgcc acgatagagg agtggcaagc agagcatgat     3720

gccatcatga ggcacatctt ggagagaccg gaccctaccg acgtcttcca gaataaggca     3780

aacgtgtgtt gggccaaggc tttagtgccg gtgctgaaga ccgctggcat agacatgacc     3840

actgaacaat ggaacactgt ggattatttt gaaacggaca aagctcactc agcagagata     3900

gtattgaacc aactatgcgt gaggttcttt ggactcgatc tggactccgg tctattttct     3960

gcacccactg ttccgttatc cattaggaat aatcactggg ataactcccc gtcgcctaac     4020

atgtacgggc tgaataaaga agtggtccgt cagctctctc gcaggtaccc acaactgcct     4080

cgggcagttg ccactggaag agtctatgac atgaacactg gtacactgcg caattatgat     4140

ccgcgcataa acctagtacc tgtaaacaga agactgcctc atgctttagt cctccaccat     4200

aatgaacacc cacagagtga cttttcttca ttcgtcagca aattgaaggg cagaactgtc     4260

ctggtggtcg gggaaaagtt gtccgtccca ggcaaaatgg ttgactggtt gtcagaccgg     4320

cctgaggcta ccttcagagc tcggctggat ttaggcatcc caggtgatgt gcccaaatat     4380

gacataatat ttgttaatgt gaggacccca tataaatacc atcactatca gcagtgtgaa     4440

gaccatgcca ttaagcttag catgttgacc aagaaagctt gtctgcatct gaatcccggc     4500

ggaacctgtg tcagcatagg ttatggttac gctgacaggg ccagcgaaag catcattggt     4560

gctatagcgc ggcagttcaa gttttcccgg gtatgcaaac cgaaatcctc acttgaagag     4620

acggaagttc tgtttgtatt cattgggtac gatcgcaagg cccgtacgca caatccttac     4680

aagctttcat caaccttgac caacatttat acaggttcca gactccacga agccggatgt     4740

gcaccctcat atcatgtggt gcgaggggat attgccacgg ccaccgaagg agtgattata     4800

aatgctgcta acagcaaagg acaacctggc ggaggggtgt gcggagcgct gtataagaaa     4860

ttcccggaaa gcttcgattt acagccgatc gaagtaggaa aagcgcgact ggtcaaaggt     4920

gcagctaaac atatcattca tgccgtagga ccaaacttca acaaagtttc ggaggttgaa     4980

ggtgacaaac agttggcaga ggcttatgag tccatcgcta agattgtcaa cgataacaat     5040

tacaagtcag tagcgattcc actgttgtcc accggcatct tttccgggaa caaagatcga     5100

ctaacccaat cattgaacca tttgctgaca gctttagaca ccactgatgc agatgtagcc     5160

atatactgca gggacaagaa atgggaaatg actctcaagg aagcagtggc taggagagaa     5220

gcagtggagg agatatgcat atccgacgac tcttcagtga cagaacctga tgcagagctg     5280

gtgagggtgc atccgaagag ttctttggct ggaaggaagg gctacagcac aagcgatggc     5340

aaaactttct catatttgga agggaccaag tttcaccagg cggccaagga tatagcagaa     5400

attaatgcca tgtggcccgt tgcaacggag gccaatgagc aggtatgcat gtatatcctc     5460

ggagaaagca tgagcagtat taggtcgaaa tgccccgtcg aagagtcgga agcctccaca     5520

ccacctagca cgctgccttg cttgtgcatc catgccatga ctccagaaag agtacagcgc     5580

ctaaaagcct cacgtccaga acaaattact gtgtgctcat cctttccatt gccgaagtat     5640

agaatcactg gtgtgcagaa gatccaatgc tcccagccta tattgttctc accgaaagtg     5700

cctgcgtata ttcatccaag gaagtatctc gtggaaacac caccggtaga cgagactccg     5760

gagccatcgg cagagaacca atccacagag gggacacctg aacaaccacc acttataacc     5820

gaggatgaga ccaggactag aacgcctgag ccgatcatca tcgaagagga agaagaggat     5880

agcataagtt tgctgtcaga tggcccgacc caccaggtgc tgcaagtcga ggcagacatt     5940

cacgggccgc cctctgtatc tagctcatcc tggtccattc ctcatgcatc cgactttgat     6000

gtggacagtt tatccatact tgacaccctg gagggagcta gcgtgaccag cggggcaacg     6060

tcagccgaga ctaactctta cttcgcaaag agtatggagt ttctggcgcg accggtgcct     6120

gcgcctcgaa cagtattcag gaaccctcca catcccgctc cgcgcacaag aacaccgtca     6180

cttgcaccca gcagggcctg ctcgagaacc agcctagttt ccaccccgcc aggcgtgaat     6240

agggtgatca ctagagagga gctcgaggcg cttaccccgt cacgcactcc tagcaggtcg     6300

gtctcgagaa ccagcctggt ctccaacccg ccaggcgtaa atagggtgat tacaagagag     6360

gagtttgagg cgttcgtagc acaacaacaa tgacggtttg atgcgggtgc atacatcttt     6420

tcctccgaca ccggtcaagg gcatttacaa caaaaatcag taaggcaaac ggtgctatcc     6480

gaagtggtgt tggagaggac cgaattggag atttcgtatg ccccgcgcct cgaccaagaa     6540

aaagaagaat tactacgcaa gaaattacag ttaaatccca cacctgctaa cagaagcaga     6600

taccagtcca ggaaggtgga gaacatgaaa gccataacag ctagacgtat tctgcaaggc     6660

ctagggcatt atttgaaggc agaaggaaaa gtggagtgct accgaaccct gcatcctgtt     6720

cctttgtatt catctagtgt gaaccgtgcc ttttcaagcc ccaaggtcgc agtggaagcc     6780

tgtaacgcca tgttgaaaga gaactttccg actgtggctt cttactgtat tattccagag     6840

tacgatgcct atttggacat ggttgacgga gcttcatgct gcttagacac tgccagtttt     6900

tgccctgcaa agctgcgcag ctttccaaag aaacactcct atttggaacc cacaatacga     6960

tcggcagtgc cttcagcgat ccagaacacg ctccagaacg tcctggcagc tgccacaaaa     7020

agaaattgca atgtcacgca aatgagagaa ttgcccgtat tggattcggc ggcctttaat     7080

gtggaatgct tcaagaaata tgcgtgtaat aatgaatatt gggaaacgtt taaagaaaac     7140

cccatcaggc ttactgaaga aaacgtggta aattacatta ccaaattaaa aggaccaaaa     7200

gctgctgctc tttttgcgaa gacacataat ttgaatatgt tgcaggacat accaatggac     7260

aggtttgtaa tggacttaaa gagagacgtg aaagtgactc caggaacaaa acatactgaa     7320

gaacggccca aggtacaggt gatccaggct gccgatccgc tagcaacagc gtatctgtgc     7380

ggaatccacc gagagctggt taggagatta aatgcggtcc tgcttccgaa cattcataca     7440

ctgtttgata tgtcggctga agactttgac gctattatag ccgagcactt ccagcctggg     7500

gattgtgttc tggaaactga catcgcgtcg tttgataaaa gtgaggacga cgccatggct     7560

ctgaccgcgt taatgattct ggaagactta ggtgtggacg cagagctgtt gacgctgatt     7620

gaggcggctt tcggcgaaat ttcatcaata catttgccca ctaaaactaa atttaaattc     7680

ggagccatga tgaaatctgg aatgttcctc acactgtttg tgaacacagt cattaacatt     7740

gtaatcgcaa gcagagtgtt gagagaacgg ctaaccggat caccatgtgc agcattcatt     7800

ggagatgaca atatcgtgaa aggagtcaaa tcggacaaat taatggcaga caggtgcgcc     7860

acctggttga atatggaagt caagattata gatgctgtgg tgggcgagaa agcgccttat     7920

ttctgtggag ggtttatttt gtgtgactcc gtgaccggca cagcgtgccg tgtggcagac     7980

cccctaaaaa ggctgtttaa gcttggcaaa cctctggcag cagacgatga acatgatgat     8040

gacaggagaa gggcattgca tgaagagtca acacgctgga accgagtggg tattctttca     8100

gagctgtgca aggcagtaga atcaaggtat gaaaccgtag gaacttccat catagttatg     8160

gccatgacta ctctagctag cagtgttaaa tcattcagct acctgagagg ggcccctata     8220

actctctacg gctaacctga atggactacg acatagtcta gtccgccaag atatcttcag     8280

ggcgcgccgc accatgtgat taattaagta acgatacagc agcaattggc aagctgctta     8340

catagaactc gcggcgattg gcatgccgct ttaaaatttt tattttattt ttcttttctt     8400

ttccgaatcg gattttgttt ttaatatttc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     8460

aaaaaaaaaa aaaaaaggaa gagcgcggcc gctcgagtct agagggcccg tttaaacccg     8520

ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt     8580

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     8640

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     8700

caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc     8760

ttctactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct     8820

ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga     8880

tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa     8940

caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     9000

tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     9060

cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag     9120

gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     9180

gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     9240

tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     9300

catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     9360

gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     9420

gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat     9480

ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     9540

tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     9600

gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     9660

tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     9720

ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc     9780

ggtatttcac accgcataca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt     9840

gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa     9900

tgcttcaata atagcacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga     9960

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt    10020

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct    10080

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc    10140

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc    10200

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc    10260

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg    10320

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt    10380

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg    10440

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg    10500

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt    10560

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag    10620

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctgggctttt    10680

gctggccttt tgctcacatg ttctt                                          10705


