               SEQUENCE LISTING

<110> Universiteit Antwerpen

<120> A novel probiotic streptococcus salivarius strain and its uses

<130> UNA-044

<150> EP20194562.3
<151> 2020-09-04

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer 515F

<400> 1
tatggtaatt gtgtgccagc mgccgcggta a                                   31


<210> 2
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer 806R

<400> 2
agtcagtcag ccggactach vgggtwtcta at                                   32


<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer 27F

<400> 3
agagtttgat cmtggctcag                                                20


<210> 4
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer 1492R

<400> 4
ggttaccttg ttacgactt                                                 19


<210> 5
<211> 77
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Bacteriocin locus 1 ctg1_271

<400> 5
Met Thr Thr Gln Thr Met Asn Asn Phe Glu Thr Leu Asp Leu Glu Ala 
1               5                   10                  15      
Leu Ala Asn Val Glu Gly Gly Asn Arg Cys Lys Asp Leu Ile Phe Gly 
            20                  25                  30          
Gly Ala Leu Thr Gly Ala Gly Ala Gly Phe Thr Gly Gly Met Ala Ala 
        35                  40                  45              
Phe Gly Val Thr Ala Phe Pro Gly Ala Phe Val Gly Ala His Val Gly 
    50                  55                  60                  
Ala Val Ala Gly Gly Leu Ala Cys Ile Gly Ala Ser Phe 
65                  70                  75          

<210> 6
<211> 581
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Bacteriocin locus 1 ctg1_272

<400> 6
Met Pro Asn Gln Asn Gln Trp Ser Val Phe Lys Arg Leu Leu Thr Tyr 
1               5                   10                  15      
Leu Lys Pro Tyr Lys Phe Leu Thr Phe Leu Ala Leu Thr Phe Leu Leu 
            20                  25                  30          
Ser Thr Thr Ile Ile Lys Ser Ile Ile Pro Leu Val Ala Ser Tyr Phe 
        35                  40                  45              
Ile Asp His Tyr Leu His Asp Met Asn Gln Ala Ala Ser Leu Ile Leu 
    50                  55                  60                  
Ile Gly Tyr Tyr Gly Leu Tyr Leu Leu Gln Thr Leu Val Gln Tyr Leu 
65                  70                  75                  80  
Gly Asn Leu Phe Phe Ala Arg Val Ser Tyr Ser Ile Val Arg Asp Ile 
                85                  90                  95      
Arg Arg Asp Ala Phe Ala Asn Met Glu Lys Leu Gly Met Ser Tyr Phe 
            100                 105                 110         
Asp Lys Thr Pro Ala Gly Ser Ile Val Ser Arg Leu Thr Asn Asp Thr 
        115                 120                 125             
Glu Thr Ile Ser Glu Met Phe Ser Gly Ile Leu Ser Ser Phe Ile Ser 
    130                 135                 140                 
Ala Ile Phe Ile Phe Val Thr Thr Leu Tyr Thr Met Met Ile Leu Asp 
145                 150                 155                 160 
Ile Arg Leu Thr Gly Leu Ile Val Leu Phe Leu Pro Leu Ile Val Leu 
                165                 170                 175     
Leu Val Asn Leu Tyr Arg Lys Lys Ser Val Val Ile Ile Glu Lys Thr 
            180                 185                 190         
Arg Ala Leu Leu Ser Asp Ile Asn Ala Lys Leu Ser Glu Ser Ile Glu 
        195                 200                 205             
Gly Ile Arg Ile Ile Gln Ala Phe Asn Gln Glu Lys Arg Leu Lys Lys 
    210                 215                 220                 
Glu Phe Asp Ala Ile Asn Glu Glu His Leu Ile Tyr Ala Ser Arg Ser 
225                 230                 235                 240 
Met Ser Leu Asp Ser Leu Phe Leu Arg Pro Ala Met Ser Leu Leu Lys 
                245                 250                 255     
Leu Leu Gly Tyr Ala Val Leu Met Ala Tyr Phe Gly Tyr Arg Gly Ile 
            260                 265                 270         
Tyr Leu Gly Ile Thr Ala Gly Thr Met Tyr Ala Phe Ile Gln Tyr Ile 
        275                 280                 285             
Asn Arg Leu Phe Asp Pro Leu Ile Glu Val Thr Gln Asn Phe Ser Thr 
    290                 295                 300                 
Leu Gln Thr Ser Met Val Ser Ala Gly Arg Val Phe Ala Leu Ile Asp 
305                 310                 315                 320 
Glu Arg Thr Tyr Glu Pro Glu Gln Arg Asp Thr Asp Ala Lys Val Thr 
                325                 330                 335     
Glu Gly Asn Ile Arg Phe Glu Asn Val Ser Phe Ser Tyr Asp Gly Lys 
            340                 345                 350         
His Gln Ile Leu Asp Asn Ile Ser Phe Ser Val Asn Lys Gly Glu Thr 
        355                 360                 365             
Ile Ala Phe Val Gly Ser Thr Gly Ser Gly Lys Ser Ser Ile Ile Asn 
    370                 375                 380                 
Val Phe Met Arg Phe Tyr Glu Phe Gln Ser Gly Arg Val Leu Leu Asp 
385                 390                 395                 400 
Gly Val Asp Ile Arg Asp Tyr Ser Gln Ala Glu Leu Arg Lys Asn Ile 
                405                 410                 415     
Gly Leu Val Leu Gln Glu Pro Phe Leu Tyr His Gly Thr Ile Lys Ser 
            420                 425                 430         
Asn Ile Ala Met Tyr Gln Asp Leu Thr Asp Glu Glu Ile Lys Ala Ala 
        435                 440                 445             
Ala Glu Phe Val Asp Ala Asn His Phe Ile Asp Lys Leu Pro Glu Gly 
    450                 455                 460                 
Tyr Asp Ala Pro Val Ser Glu Arg Gly Ser Ser Phe Ser Thr Gly Gln 
465                 470                 475                 480 
Arg Gln Leu Leu Ala Phe Ala Arg Thr Val Ala Ser Gln Pro Lys Ile 
                485                 490                 495     
Leu Ile Leu Asp Glu Ala Thr Ala Asn Ile Asp Ser Glu Thr Glu Ala 
            500                 505                 510         
Ile Val Gln Asn Ser Leu Ala Lys Met Arg Gln Gly Arg Thr Thr Ile 
        515                 520                 525             
Ala Ile Ala His Arg Leu Ser Thr Ile Gln Asp Ala Asn Cys Ile Tyr 
    530                 535                 540                 
Val Leu Asp Lys Gly Arg Ile Ile Glu His Gly Ser His Glu Glu Leu 
545                 550                 555                 560 
Leu Ala Leu Gly Gly Thr Tyr His Lys Met Tyr Ser Leu Gln Ala Gly 
                565                 570                 575     
Ala Met Glu Gly Ala 
            580     

<210> 7
<211> 585
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Bacteriocin locus 1 ctg1_273

<400> 7
Leu Phe Phe Lys Glu Ser Ile Ile Ala Lys Leu Trp Trp Phe Phe Lys 
1               5                   10                  15      
Leu Glu Lys Arg Arg Tyr Ile Val Gly Ile Leu Ala Leu Ser Leu Val 
            20                  25                  30          
Ser Val Leu Asn Leu Ile Pro Pro Met Val Met Gly Arg Val Ile Asp 
        35                  40                  45              
Ala Ile Thr Ser Gly Lys Leu Thr Asn Gln Ile Leu Leu Leu Asn Leu 
    50                  55                  60                  
Val Tyr Leu Leu Leu Ala Ala Ile Gly Met Tyr Tyr Leu Arg Tyr Val 
65                  70                  75                  80  
Trp Arg Met Tyr Ile Leu Ala Thr Ser Tyr Arg Leu Gly Gln Ile Met 
                85                  90                  95      
Arg Ser Arg Leu Phe Glu His Phe Thr Lys Met Ser Pro Ser Phe Tyr 
            100                 105                 110         
Gln Lys Tyr Arg Thr Gly Asp Leu Met Ala His Ala Thr Asn Asp Ile 
        115                 120                 125             
Asn Ser Leu Thr Arg Leu Ala Gly Gly Gly Val Met Ser Ala Val Asp 
    130                 135                 140                 
Ala Ser Ile Thr Ala Leu Val Thr Leu Ile Thr Met Phe Phe Ser Ile 
145                 150                 155                 160 
Ser Trp Gln Met Thr Leu Val Ala Val Leu Pro Leu Pro Phe Met Ala 
                165                 170                 175     
Phe Ala Thr Ser Arg Leu Gly Arg Lys Thr His Lys Ala Phe Gly Glu 
            180                 185                 190         
Ser Gln Ala Ala Phe Ser Glu Leu Asn Asn Lys Val Gln Glu Ser Val 
        195                 200                 205             
Ser Gly Ile Lys Leu Thr Lys Ser Phe Gly Tyr Gln Glu Ala Glu Leu 
    210                 215                 220                 
Gln Ser Phe Gln Glu Thr Asn Lys Met Thr Phe Lys Lys Asn Met His 
225                 230                 235                 240 
Thr Met Lys Tyr Asp Ser Leu Phe Asp Pro Leu Val Leu Leu Phe Val 
                245                 250                 255     
Gly Ser Ser Tyr Val Leu Thr Leu Leu Val Gly Ala Phe Met Ile Gln 
            260                 265                 270         
Ala Gly Gln Ile Thr Val Gly Asn Leu Val Thr Phe Ile Thr Tyr Leu 
        275                 280                 285             
Asp Met Leu Val Trp Pro Leu Met Ala Ile Gly Phe Leu Phe Asn Ile 
    290                 295                 300                 
Thr Gln Arg Gly Asn Val Ser Tyr Gln Arg Ile Glu Asn Leu Leu Glu 
305                 310                 315                 320 
Gln Glu Ser Asp Val Gln Asp Pro Lys His Pro Leu Pro Ser Ile Glu 
                325                 330                 335     
Asn Gly Arg Leu Glu Tyr Ala Ile Asp Arg Phe Ala Phe Glu Asp Glu 
            340                 345                 350         
Asp Thr Leu Arg Asp Ile His Phe Thr Leu Asp Lys Gly Gln Thr Leu 
        355                 360                 365             
Gly Leu Val Gly Gln Thr Gly Ser Gly Lys Thr Ala Leu Val Lys Leu 
    370                 375                 380                 
Leu Leu Arg Glu His Asp Val Asn Gln Gly Ala Ile Tyr Leu Asn Gly 
385                 390                 395                 400 
His Asp Ile Arg Asp Tyr Arg Leu Ser Asp Leu Arg Ser Leu Met Gly 
                405                 410                 415     
Tyr Val Pro Gln Asp Gln Phe Leu Phe Ala Ser Ser Ile Leu Asp Asn 
            420                 425                 430         
Val Arg Phe Gly Asn Pro Asp Leu Ala Phe Glu Lys Val Glu Glu Ala 
        435                 440                 445             
Thr Lys Leu Ala Gln Val Tyr Asp Asp Ile Lys Ala Met Pro Glu Gly 
    450                 455                 460                 
Phe Glu Thr Ile Ile Gly Glu Lys Gly Val Ser Leu Ser Gly Gly Gln 
465                 470                 475                 480 
Lys Gln Arg Leu Ala Met Ser Arg Ala Met Ile Leu Asp Pro Asp Ile 
                485                 490                 495     
Leu Ile Leu Asp Asp Ser Leu Ser Ala Val Asp Ala Lys Thr Glu His 
            500                 505                 510         
Ala Ile Ile Asp Asn Leu Lys His Thr Arg Lys Asp Lys Thr Thr Ile 
        515                 520                 525             
Ile Thr Ala His Arg Leu Ser Ala Val Val His Ala Asp Leu Val Leu 
    530                 535                 540                 
Val Met Gln Asn Gly Arg Ile Ile Glu Arg Gly Arg His Glu Asp Leu 
545                 550                 555                 560 
Leu Ala Gln Gly Gly Trp Tyr Ala Lys Thr Tyr Glu Ser Gln Gln Leu 
                565                 570                 575     
Glu Met Glu Gly Gly Glu Val Asp Ala 
            580                 585 

<210> 8
<211> 358
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_139

<400> 8
Val Glu Lys Ser Leu Thr Phe Asn Gln Leu Val Lys Gln Thr Tyr Phe 
1               5                   10                  15      
Lys Ile Leu Arg Gln Phe Leu Ile Asn Leu Phe Leu Phe Tyr Leu Leu 
            20                  25                  30          
Pro Met Leu Leu Thr Ala Phe Thr Asn Ile Thr Glu Lys Asn Ala Gly 
        35                  40                  45              
Ala Phe Thr Ile Tyr Phe Ser Thr Ala Ser Trp Ile Tyr Ile Ser Leu 
    50                  55                  60                  
Ile Leu Leu Phe Ser Ile Thr Lys Asn Met Arg Asn Leu Leu Glu Lys 
65                  70                  75                  80  
Ile Lys Gln Glu Thr Ile Ile Val Tyr Asn Gln Ser Leu Leu Gly Asp 
                85                  90                  95      
Thr Asn Glu Asn Asn Ser Ala Lys Leu Thr Leu Ile Glu Leu Ile Glu 
            100                 105                 110         
Thr Arg Gln Lys Ile Gln Glu Met Gln Ala Thr Ile Lys Lys Ile Ile 
        115                 120                 125             
Gln Ser Glu Lys Gln Gln Lys Gln Glu Leu Met Phe Gln Val Ser Ala 
    130                 135                 140                 
Ala Ala His Asp Leu Lys Thr Pro Leu Thr Val Ile Gln Gly Asn Ala 
145                 150                 155                 160 
Gln Phe Leu Gln Ser Met Asp Ile Pro Gly Asn Ile Glu Gln Cys Leu 
                165                 170                 175     
Glu Asp Ile Glu Leu Ala Ser Gln Gln Leu Asn Leu Tyr Phe Asn Gln 
            180                 185                 190         
Leu Ile Asn Tyr Ser Lys Thr Phe Tyr Asn Asp Thr Ser Asn Trp Glu 
        195                 200                 205             
Ser Leu Ser Ser Asp Tyr Leu Phe Glu Leu Phe Glu Gln Glu Ile Lys 
    210                 215                 220                 
Leu Ile Thr Asp Lys Lys Ala Asp Ile Gln Phe Ser Asn Asn Leu Pro 
225                 230                 235                 240 
Thr Ser Ile Tyr Leu Thr Leu Asn Leu Asn Leu Phe Leu Arg Ala Thr 
                245                 250                 255     
Leu Asn Ile Ile Asn Asn Ala Ile Asp His Ser Gln Ser Ser Tyr Pro 
            260                 265                 270         
Thr Ile Lys Val Asn Tyr Lys Phe Ile Asp Asn Gln Phe Tyr Ile Ser 
        275                 280                 285             
Ile Trp Asn Ser Asp Ser Ser Phe Thr Glu Asn Leu Leu Glu Lys Tyr 
    290                 295                 300                 
Gly Leu Leu Phe Tyr Gln Asp Lys Gln Ala Thr Asn Ser Glu Gln Arg 
305                 310                 315                 320 
Ser Asn Phe Gly Ile Gly Leu Ala Phe Val Lys Arg Val Ala Lys Ile 
                325                 330                 335     
His Gly Gly Gln Val Asn Leu Ser Asn Cys Asn Asn Gly Ala Leu Val 
            340                 345                 350         
Thr Ile Cys Ile Pro Val 
        355             

<210> 9
<211> 223
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_140

<400> 9
Met Lys Tyr Lys Ile Leu Val Ile Asp Asp Asp Lys Ser Ile Leu Arg 
1               5                   10                  15      
Leu Val Arg Asn Val Leu Glu Ser Lys Asn Phe Val Val Asp Thr Leu 
            20                  25                  30          
Thr Ser Ile Pro Asp Ile Asp Ile Cys His Phe Ile Gly Tyr Asp Leu 
        35                  40                  45              
Ile Ile Leu Asp Ile Met Met Pro His Ser Gly Ile Glu Ile Cys Arg 
    50                  55                  60                  
Tyr Ile Arg Glu Phe Ile Ser Val Pro Ile Ile Phe Leu Thr Ala Lys 
65                  70                  75                  80  
Asn Leu Glu Glu Asp Val Val Cys Gly Ile Gln Ser Gly Ala Asp Asp 
                85                  90                  95      
Tyr Ile Thr Lys Pro Phe Ser Ile Pro Glu Leu Val Ala Arg Val Gln 
            100                 105                 110         
Met His Ile Arg Arg Glu Val Arg Asn Asn Asn Ser Asn Glu Ile Leu 
        115                 120                 125             
Gln Phe Gly Pro Ile Thr Ile Asn Lys Ile Lys Glu Glu Leu Arg Val 
    130                 135                 140                 
Ala Asp Val His Val Pro Thr Thr Lys Arg Glu Phe Gln Leu Ile Leu 
145                 150                 155                 160 
Leu Leu Ser Ser Asn Pro Asn Lys Ile Phe Ser Asn Glu Glu Leu Phe 
                165                 170                 175     
Asp Tyr Leu Tyr Pro His Asp Ser Glu Thr Gln Gly Arg Ser Ile Thr 
            180                 185                 190         
Glu Tyr Val Tyr Gln Val Arg Gln Lys Leu Lys Pro Tyr Glu Leu Asn 
        195                 200                 205             
Pro Ile Lys Thr Ile Trp Gly Gly Gly Tyr Gln Trp Arg Ser Leu 
    210                 215                 220             

<210> 10
<211> 587
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_142

<400> 10
Met Lys Val Asn Val Ile Leu Ser Lys Asn Gln Ile Ile Thr Thr Asp 
1               5                   10                  15      
Thr Leu Ile Asp Glu Leu Val Val Ser Glu Glu Leu Lys Thr Tyr Thr 
            20                  25                  30          
Ala Asp Asn Leu Leu Met Ile Val Lys Pro His Lys Asn Ser Glu Asp 
        35                  40                  45              
Ile Gly Arg Phe Leu Ala Lys Glu Asn Leu Asn Ile Glu Met Ile Glu 
    50                  55                  60                  
Ser Asn Leu Leu Glu Leu Lys Lys Asn Ala Ser Phe Val Ala Ile Thr 
65                  70                  75                  80  
Lys Glu Asn His Phe Val Ile Gly Gly Asp Phe Asn Asn His Asn Pro 
                85                  90                  95      
Ile Tyr Tyr Leu Glu Lys Asp Glu Glu Leu Phe Ile Ser Phe Tyr Pro 
            100                 105                 110         
Glu Leu Ile Ala Ser Phe Thr Lys Gln Lys Leu Asn Arg Thr Tyr Phe 
        115                 120                 125             
Ala Leu Arg Ile Val Asn Phe Asp Thr Val Tyr Pro Phe Thr Glu Leu 
    130                 135                 140                 
Thr Pro Trp Asp Asn Val Lys Leu Val Asn Ser Arg Lys Ile Leu Val 
145                 150                 155                 160 
Ser Leu Glu Gly Gln Val Lys Gln Val Phe Pro Trp Arg Ser Asn Gly 
                165                 170                 175     
Glu Asn Lys Thr Ile Ala Gly Val Ala Gly Glu Leu Ser Lys Asn Leu 
            180                 185                 190         
Asn Ser Val Leu Arg Lys Tyr Leu Glu Asn Gln Pro Lys Ile Ser Ala 
        195                 200                 205             
Asp Leu Ser Gly Gly Leu Asp Ser Thr Ser Leu Cys Tyr Phe Cys Lys 
    210                 215                 220                 
Asn Leu Gly Ile Glu Leu Asn Thr Val Phe Leu Ser Pro Glu Asp Gly 
225                 230                 235                 240 
Ala Asp Ser Asp Val Val Trp Ser Asn Arg Ala Ser Arg Asp Val Ser 
                245                 250                 255     
Ala Glu His Val Ile Leu Pro Tyr Ser Ser Thr Val Thr Leu Ser Thr 
            260                 265                 270         
Leu Asp Pro Ile Ala Ile Tyr Asn Ala Leu Pro Phe Gly Pro Ser Glu 
        275                 280                 285             
Ser Phe Arg Tyr Ile Asn Leu Ala Arg Lys Ile Ile Glu Ile Gly Leu 
    290                 295                 300                 
Glu Asn Asp Ile Ser Thr His Ile Asn Gly His Gly Gly Asp Glu Leu 
305                 310                 315                 320 
Phe Gly Pro Leu Ser Thr Met Ala Trp Ser Leu Tyr His Ser Asn Tyr 
                325                 330                 335     
Pro Asn Arg Ile Arg Asn Leu Tyr Gly Phe Ala Arg Leu Asn Lys Phe 
            340                 345                 350         
Asn Leu Phe Lys Phe Phe Lys Ser Leu Thr Asp Asn Thr Ser Leu Ser 
        355                 360                 365             
Asp Asn Leu Arg Asn Leu Val Cys Gln Glu Val Glu Gln Val Asp Phe 
    370                 375                 380                 
Asp Ile Lys Tyr Ser Ser Lys Trp Ile Ser Thr Leu Ser Val Glu Ser 
385                 390                 395                 400 
Phe Val Thr Gln Thr Ala Arg Asp Leu Ile Thr Arg Cys Ala Thr Ser 
                405                 410                 415     
Tyr Ala Glu Lys Asp Ile Glu Ser Tyr Ser Asp Asp Lys Ser Ile His 
            420                 425                 430         
Lys Ile Leu Glu Glu Ile Glu Ser His Thr Leu Leu Gln Arg Asp Leu 
        435                 440                 445             
Asn Ala Met Val Pro Thr Asp Lys Tyr Phe Arg Phe Leu Ser Pro Tyr 
    450                 455                 460                 
Thr Asn Tyr Asp Ile Val Glu Ser Ala Leu Lys Tyr Asn Ile Asp Glu 
465                 470                 475                 480 
Arg Phe Asn Ala Lys Val Thr Lys Pro Met Leu Ala Glu Ile Arg Pro 
                485                 490                 495     
Ser Ser Met Ser Leu Glu Tyr Phe Gln Arg Arg Asp Lys Gly Glu Tyr 
            500                 505                 510         
Ser Lys Pro Thr Phe Thr Glu Tyr Ser Lys Arg Lys Lys Ile Leu Gln 
        515                 520                 525             
Lys Ile Phe Ser Glu Glu Cys Leu Ile Tyr Lys Leu Gly Leu Val Asp 
    530                 535                 540                 
Arg Val Asp Leu Leu Glu Ser Leu Asn Lys Phe Ser Ala Asp Gly Glu 
545                 550                 555                 560 
Leu Leu Glu Asn Val Met Arg Leu Gln Ile Ile Glu Phe Trp Leu Arg 
                565                 570                 575     
Gly Tyr Cys Glu Ser Glu Gly Glu Tyr Glu Ile 
            580                 585         

<210> 11
<211> 139
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_144

<400> 11
Met Ser Ser Pro Val Arg Met Glu Gln Arg Thr Lys Leu Ser Val Lys 
1               5                   10                  15      
Asn Lys Ile Thr Ala Leu Phe Cys Ala Asn Val Ser Phe Phe Leu Ile 
            20                  25                  30          
Lys Leu Pro Pro Lys Lys Leu Ser Glu Val Ile Glu Lys Leu Ser Lys 
        35                  40                  45              
Asn Thr Arg Lys Ala Leu Pro Lys Glu Val Glu Ser Trp Arg Thr Ser 
    50                  55                  60                  
Ile Asn Ser Ile Asn Val Arg Cys Ala Gly Asn Gly Cys Leu Gln Arg 
65                  70                  75                  80  
Ser Val Ala Val Met Leu Arg Gly Ile Ile Ala Arg Arg Thr Pro Asp 
                85                  90                  95      
Trp Val Ser Gly Phe Gln Val Ser Pro Phe Ile Ala His Ala Trp Val 
            100                 105                 110         
Glu Val Asp Gly Ile Pro Ile Gly Glu Glu Met Asp Leu Ser Asn Phe 
        115                 120                 125             
Gln Lys Ile Leu Phe Val Lys Gly Gly Gln Arg 
    130                 135                 

<210> 12
<211> 235
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_145

<400> 12
Met Ile Lys Ile Glu Asn Val Ser Lys Ala Phe Arg Glu Lys Ile Val 
1               5                   10                  15      
Leu Ser Lys Ile Ser Phe Thr Ala Asn Glu Gly Glu Val Thr Ala Leu 
            20                  25                  30          
Ile Gly Pro Asn Gly Ser Gly Lys Ser Thr Leu Leu Lys Ile Leu Leu 
        35                  40                  45              
Gly Leu Ile Ser Thr Asp Glu Gly Arg Ala Thr Phe Asp Gly Lys Asn 
    50                  55                  60                  
Tyr Gly Glu Leu Gly Lys Tyr Pro Phe Arg Tyr Val Gly Thr Phe Leu 
65                  70                  75                  80  
Asp Ser Phe Gln Pro Asn Pro Thr Arg Thr Ala Tyr Asn His Leu Arg 
                85                  90                  95      
Trp Ile Ala Leu Thr Ser Gly Ile Ser Lys Gln Arg Cys Leu Glu Cys 
            100                 105                 110         
Leu Lys Ile Val Gly Leu Gln Asp Val Gly Asn Lys Lys Ile Lys Asp 
        115                 120                 125             
Tyr Ser Leu Gly Met Lys Gln Arg Leu Gly Leu Ala Thr Ala Ile Leu 
    130                 135                 140                 
Ala Asn Pro Lys Ile Leu Ile Leu Asp Glu Pro Ile Asn Gly Leu Asp 
145                 150                 155                 160 
Pro Asp Gly Ile Arg Trp Val Arg Glu Phe Leu Arg Glu Phe Val Lys 
                165                 170                 175     
Asp Asp Lys Ile Val Leu Ile Thr Ser His Tyr Met Asn Glu Leu Glu 
            180                 185                 190         
Leu Thr Val Asp Lys Ile Val Gly Leu Ser Asn Gly Lys Val Val Ile 
        195                 200                 205             
Asp Gly Ser Ser Lys Asp Val Leu Glu Glu Tyr Gly Ser Phe Glu Glu 
    210                 215                 220                 
Ala Tyr Phe Arg Ser Val Arg Asn Glu Met Arg 
225                 230                 235 

<210> 13
<211> 580
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Lassopeptide ctg2_148

<400> 13
Met Asp Ser Gln Gln His Ser Glu Val Ser Phe Arg Glu Phe Leu Arg 
1               5                   10                  15      
Leu Leu Lys Pro His Lys Ile Ser Ile Ser Ile Ala Leu Ile Ile Ala 
            20                  25                  30          
Leu Ile Ala Ser Thr Leu Ser Ile Leu Gln Pro Ile Leu Leu Ser Lys 
        35                  40                  45              
Ile Ile Asp Asn Ile Asn Asn Thr Ile Leu Lys Lys Thr Val Ile Leu 
    50                  55                  60                  
Phe Ser Met Ile Val Leu Ser Ser Ala Ile Leu Asn Ser Ile Lys Gln 
65                  70                  75                  80  
Tyr Ile Leu Glu Ser Ile Ser Glu Asn Leu Val Asn Ser Leu Arg Ile 
                85                  90                  95      
Gln Ile Val Asn Arg Ser Ile Lys Tyr Lys Ile Glu Val Phe Asp Thr 
            100                 105                 110         
Arg Lys Ile Gly Asp Leu Ala Ser Ile Leu Ser Thr Asp Thr Ala Gln 
        115                 120                 125             
Leu Arg Gly Ile Leu Ser Gln Gly Ile Val Glu Leu Val Ser Gln Thr 
    130                 135                 140                 
Phe Thr Met Leu Phe Ala Leu Ile Met Met Phe Tyr Leu Asp Ile Gln 
145                 150                 155                 160 
Leu Phe Ser Leu Ser Leu Leu Ala Val Leu Ser Leu Leu Ile Cys Gly 
                165                 170                 175     
Leu Leu Leu Gly Lys Arg Thr Arg Pro Ile Ala Lys Asp Leu Gln Glu 
            180                 185                 190         
Val Val Gly Glu Leu Ser Ser Glu Leu Glu Arg Thr Leu Arg Gly Val 
        195                 200                 205             
Arg Thr Ile Arg Ala Phe Leu Ser Glu Asn Val Phe Val Glu Arg Met 
    210                 215                 220                 
Ser Ser Val Val Thr Ser Ala Thr Lys Ile Gly Lys Lys Val Ala Ile 
225                 230                 235                 240 
Phe Lys Ser Ile Ile Ser Gly Phe Ser Asn Ile Ala Leu Gln Ile Met 
                245                 250                 255     
Leu Ile Ile Ile Ile Gly Val Gly Ala Ile Arg Val Ala Thr Gly Val 
            260                 265                 270         
Ile Ser Ile Gly Ser Leu Ser Ala Phe Ile Met Tyr Val Met Leu Val 
        275                 280                 285             
Leu Thr Pro Ala Ala Met Leu Gly Gly Val Leu Ala Ser Ile Asn Glu 
    290                 295                 300                 
Gly Leu Gly Ala Tyr Ser Arg Ile Lys Val Ala Leu Glu Leu Pro Ile 
305                 310                 315                 320 
Glu Glu Asp Ile Asn Ser Leu Asp Val Leu Glu Lys Lys Glu Asn Gln 
                325                 330                 335     
Ile Leu Gln Ile Lys Lys Leu Ser Phe Lys Tyr Asn Asp Asn Phe Asp 
            340                 345                 350         
Lys Asn Ile Leu Asn Lys Ile Asp Ile Ser Val Asn Gly Pro Gly Val 
        355                 360                 365             
Thr Ala Leu Val Gly Pro Ser Gly Ser Gly Lys Thr Thr Ile Phe Glu 
    370                 375                 380                 
Leu Ile Glu Arg Phe Tyr Asp Phe Asp Asp Gly Thr Ile Glu Leu Tyr 
385                 390                 395                 400 
Gly Asn Asn Ile Tyr Asp Leu Pro Ile Lys Val Leu Arg Ser Asn Ile 
                405                 410                 415     
Thr Phe Val Glu Gln Asn Ala Ser Ile Phe Ser Gly Thr Val Leu Glu 
            420                 425                 430         
Asn Leu Gln Ile Ala Asn Lys Asn Val Ser Arg Leu Asp Cys Leu His 
        435                 440                 445             
Ala Leu Asn Leu Val Asn Leu Phe Gln Asp Ile Pro Ser Glu Glu Ile 
    450                 455                 460                 
Leu Asp Arg Val Ile Gly Glu Ser Gly Ile Thr Leu Ser Gly Gly Glu 
465                 470                 475                 480 
Lys Gln Arg Leu Ser Leu Ala Arg Ala Leu Ile Ser Pro Asn Asp Ile 
                485                 490                 495     
Ile Leu Leu Asp Glu Thr Thr Ser Asn Leu Asp Ser Ile Asn Glu Ser 
            500                 505                 510         
Lys Ile His Gln Leu Ile Lys Asp Leu Ser Arg Arg Lys Thr Val Leu 
        515                 520                 525             
Ile Ile Ala His Arg Leu Ser Thr Ile Gln Asn Ala Asp Ser Ile Tyr 
    530                 535                 540                 
Leu Ile Ser Glu Gly Glu Val Asp Asp Cys Gly Thr His Gln Glu Leu 
545                 550                 555                 560 
Leu Ser Arg Asn Lys Leu Tyr Lys Glu Leu Val Glu Thr Gln Phe Ile 
                565                 570                 575     
Asn Lys Glu Thr 
            580 

<210> 14
<211> 698
<212> PRT
<213> Streptococcus salivarius


<220> 
<223> Bacteriocin locus 2 ctg4_81

<400> 14
Met Ser Lys Tyr Pro Tyr Ile Ser Gln Val Asp Gln Arg Asp Cys Gly 
1               5                   10                  15      
Val Ala Thr Leu Ala Met Ile Leu Lys His Tyr Gly Ser Ser Tyr Ser 
            20                  25                  30          
Leu Ala Tyr Leu Arg Glu Leu Ala Gln Thr Ser Arg Glu Gly Thr Ser 
        35                  40                  45              
Ala Leu Gly Leu Val Glu Ala Ala Lys Gln Leu Gly Leu Glu Thr Gln 
    50                  55                  60                  
Ala Ile Arg Ala Asp Leu Asp Leu Phe Lys Gln Glu Asn Leu Ser Tyr 
65                  70                  75                  80  
Pro Phe Ile Val His Val Val Lys Glu Gly Gly Leu Gln His Tyr Tyr 
                85                  90                  95      
Thr Val Phe Gly Gln Ile Lys Gly Gln Leu Val Ile Gly Asp Pro Asp 
            100                 105                 110         
Pro Ser Lys Lys Val Ile Lys Met Pro Leu Glu Glu Phe Ser Lys Glu 
        115                 120                 125             
Trp Thr Gly Val Ala Leu Phe Phe Val Pro Gly Glu Ala Tyr Thr Lys 
    130                 135                 140                 
Tyr Lys Glu Asp Val Pro Gly Leu Leu Ser Phe Leu Pro Ile Leu Phe 
145                 150                 155                 160 
Arg Arg Lys Gly Leu Ile Ala Val Ile Val Leu Leu Ser Phe Leu Val 
                165                 170                 175     
Thr Leu Val Asn Ile Ile Gly Ser Tyr Tyr Leu Gln Ala Ile Ile Asp 
            180                 185                 190         
Arg Leu Ile Pro Gln Gly Ala Tyr Asn Leu Leu Thr Met Ile Ser Leu 
        195                 200                 205             
Gly Leu Cys Ile Ser Tyr Leu Ala Gln Gln Val Phe Thr Phe Phe Lys 
    210                 215                 220                 
Asp Tyr Leu Leu His Arg Leu Gly Asn Tyr Leu Ser Ile Ala Val Ile 
225                 230                 235                 240 
Leu Pro Tyr Ile Lys His Val Leu Ser Leu Pro Ile Ser Phe Phe Ser 
                245                 250                 255     
Ser Arg Arg Thr Gly Glu Ile Thr Ser Arg Phe Gly Asp Ala Asn Thr 
            260                 265                 270         
Ile Ile Asp Ala Leu Ala Ser Thr Ile Leu Ser Ile Phe Leu Asp Val 
        275                 280                 285             
Thr Ile Val Met Thr Leu Ala Val Ala Leu Ile Val Gln Asn Gln Ser 
    290                 295                 300                 
Leu Phe Leu Met Thr Leu Thr Val Val Pro Ile Tyr Val Leu Ile Ile 
305                 310                 315                 320 
Val Ala Phe Tyr Lys Leu Phe Glu Lys Glu Asn Tyr Gln Leu Met Glu 
                325                 330                 335     
Ala Asn Ser Gln Val Asn Thr Ala Val Ile Asp Asp Leu Arg Gly Ile 
            340                 345                 350         
Glu Thr Leu Lys Ser Leu Arg Val Glu Glu Arg Arg Tyr Arg Glu Ile 
        355                 360                 365             
Glu Val Lys Phe His Asp Tyr Leu Lys Lys Ser Leu Ser Lys Ala Lys 
    370                 375                 380                 
Trp Gln Leu Thr Gln Asp Gly Leu Lys Thr Gly Val Gln Leu Val Ser 
385                 390                 395                 400 
Asn Val Phe Ile Leu Trp Tyr Gly Ala Gln Leu Val Met Glu Gly Gln 
                405                 410                 415     
Leu Ser Ala Gly Gln Leu Ile Thr Tyr Asn Met Leu Leu Asn Tyr Phe 
            420                 425                 430         
Thr Thr Pro Leu Ile Asn Ile Ile Asn Leu Gln Ser Lys Ile Gln Gln 
        435                 440                 445             
Ala Lys Val Ala Asn Asn Arg Leu Gln Glu Val Tyr Val Val Asp Lys 
    450                 455                 460                 
Glu Glu Lys Gly Lys Leu Arg Glu Leu Ser Phe Lys Gln Leu Ala Phe 
465                 470                 475                 480 
Lys Gly Val Ser His Arg Phe Ser Tyr Gln Gln Glu Thr Leu Ser Lys 
                485                 490                 495     
Ile Asp Leu Thr Ile His Lys Gly Glu Lys Ile Ala Leu Met Gly Lys 
            500                 505                 510         
Ser Gly Ser Gly Lys Thr Thr Leu Ala Lys Met Leu Ser Gly Tyr Tyr 
        515                 520                 525             
Thr Lys Ser Ser Gly Arg Val Thr Leu Asp Lys Asp Ala Ile Ser His 
    530                 535                 540                 
Ala Glu Leu Arg Gln Leu Val Thr Tyr Val Pro Gln Gln Thr Tyr Val 
545                 550                 555                 560 
Phe Thr Gly Thr Ile Leu Glu Asn Leu Leu Leu Gly Phe Glu Gly Glu 
                565                 570                 575     
Val Asp Glu Lys Lys Leu Leu Lys Val Cys Gln Gln Ala Asp Ile Leu 
            580                 585                 590         
Glu Asp Ile Gln Lys Met Pro Leu Gly Phe Gln Thr Gln Val Ser Glu 
        595                 600                 605             
Asp Gly Gly Leu Ser Gly Gly Gln Lys Gln Arg Leu Ala Ile Ala Arg 
    610                 615                 620                 
Ala Leu Leu Ser Lys Gln Pro Ile Leu Ile Phe Asp Glu Ala Thr Ser 
625                 630                 635                 640 
Gly Leu Asp Ser Asp Thr Glu Ser Arg Val Met Ala Asn Leu Ala Lys 
                645                 650                 655     
Ile Lys Arg Thr Met Ile Phe Ile Ala His Arg Asn Ser Val Arg Gln 
            660                 665                 670         
His Val Ser Arg Val Val Thr Met Val Ser Gly Gln Ile Glu Ser Asp 
        675                 680                 685             
Ser Pro Asn Phe Asn Leu Phe Gln Phe Ile 
    690                 695             

