                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       Patel, Ami
       Weiner, David
       Muthumani, Kar
       Reuschel, Emma
 
<120>  Intradermal MERS-CoV Vaccine

<130>  206193-0073-00WO

<150>  US 63/069,868
<151>  2020-08-25

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  4068
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MERS-CoV Consensus Spike Antigen

<400>  1
atggactgga cttggattct gttcctggtc gccgccgcaa ctcgcgtgca tagctacgtg       60

gatgtcggcc cagactctgt gaagagtgct tgcatcgagg tcgatattca gcagacattc      120

tttgacaaga cttggcctcg accaatcgac gtgagcaaag ccgacggcat catctacccc      180

cagggaagga cttatagtaa catcaccatt acataccagg gcctgttccc ttatcagggc      240

gaccacggag atatgtacgt gtattccgcc ggacatgcta ccgggaccac accacagaaa      300

ctgtttgtgg caaattattc tcaggacgtg aagcagttcg ccaacgggtt tgtggtcaga      360

atcggcgccg ctgcaaactc cactggcacc gtgatcattt ccccctctac cagtgccaca      420

atccggaaaa tctaccctgc ttttatgctg ggcagctccg tgggaaactt ctctgatggg      480

aagatgggcc gcttctttaa tcacaccctg gtgctgctgc cagacggatg cgggacactg      540

ctgagggcct tctactgtat cctggagccc agaagcggaa atcactgccc tgctgggaac      600

tcatacacca gctttgccac ttatcatacc cctgctacag actgttccga tggcaattat      660

aaccggaatg cctccctgaa ctctttcaag gaatacttta atctgcgcaa ctgcacattc      720

atgtacactt ataatatcac cgaggatgaa attctggagt ggttcgggat cacacagact      780

gctcagggcg tgcacctgtt ttctagtcgc tacgtcgatc tgtatggcgg aaacatgttc      840

cagtttgcca ccctgccagt gtacgacaca attaagtact atagcatcat tccccatagt      900

atccgatcaa ttcagagcga caggaaggct tgggccgctt tctacgtgta taaactgcag      960

cccctgacct tcctgctgga tttttctgtg gacggataca tcaggagagc cattgattgc     1020

gggtttaacg acctgagcca gctgcactgt tcctatgaat ctttcgatgt ggagtccggg     1080

gtgtactctg tctcaagctt tgaggctaag ccatcaggga gcgtggtcga gcaggcagaa     1140

ggcgtggagt gcgacttcag tcccctgctg tcaggcacac cccctcaggt gtacaatttc     1200

aaaagactgg tctttactaa ctgtaattac aacctgacca agctgctgag tctgttctca     1260

gtgaacgact ttacctgcag ccagatctcc cctgcagcca ttgccagcaa ttgttattcc     1320

tctctgatcc tggattactt ctcctatccc ctgtctatga aaagtgacct gtcagtgagt     1380

tcagcaggcc ctatctctca gtttaattac aagcagtcct tctctaaccc cacttgcctg     1440

attctggcca ccgtgcctca caacctgact accatcacaa agccactgaa atactcctat     1500

attaacaagt gcagcagact gctgtccgac gatcggactg aagtgcctca gctggtcaat     1560

gccaaccagt actctccatg cgtgagcatc gtcccctcaa ccgtgtggga agacggagat     1620

tactatcgga agcagctgag ccccctggag ggcggcggct ggctggtggc aagtgggtca     1680

acagtcgcca tgactgagca gctgcagatg ggcttcggaa tcaccgtgca gtacggcacc     1740

gatacaaatt ctgtctgtcc taagctggaa tttgctaacg acacaaaaat tgcaagtcag     1800

ctgggcaatt gcgtggagta ctctctgtat ggagtgagtg ggagaggcgt cttccagaac     1860

tgtacagccg tgggcgtccg acagcagagg ttcgtgtacg atgcttatca gaacctggtc     1920

ggctactatt ccgacgatgg aaattactat tgcctgcgag catgcgtgag cgtcccagtg     1980

tccgtcatct acgacaagga aactaaaacc cacgcaaccc tgttcggctc agtggcctgc     2040

gagcatatta gctccaccat gagccagtat agcagatcca cacggtccat gctgaaacgg     2100

cgcgactcta catacggacc cctgcagact cctgtggggt gcgtgctggg cctggtgaac     2160

tctagtctgt tcgtcgaaga ttgcaagctg ccactgggac agtctctgtg cgcactgcca     2220

gacacaccca gtacactgac tccacgcagc gtgcgatccg tcccaggaga gatgagactg     2280

gcaagcatcg ccttcaatca ccctattcag gtggatcagc tgaactcaag ctactttaag     2340

ctgtcaatcc caacaaactt cagctttggc gtgactcagg agtatatcca gacaactatt     2400

cagaaggtga ccgtcgactg caaacagtac gtgtgcaatg gattccagaa atgcgaacag     2460

ctgctgcggg agtatgggca gttttgttcc aagatcaatc aggcactgca tggcgccaac     2520

ctgcgccagg acgatagtgt gcgaaacctg ttcgcctcag tcaagtcctc tcagagttca     2580

cctatcattc cagggttcgg cggcgacttc aacctgaccc tgctggaacc cgtgagcatc     2640

agtaccggca gcaggagcgc cagaagcgca atcgaggatc tgctgtttga caaagtgacc     2700

attgccgacc caggatacat gcaggggtat gacgattgca tgcagcaggg accagcatcc     2760

gctcgcgatc tgatctgtgc tcagtacgtg gcagggtata aggtcctgcc acccctgatg     2820

gacgtgaaca tggaagctgc atatactagc tccctgctgg ggagcattgc aggagtggga     2880

tggaccgctg gactgtctag tttcgccgct atcccatttg ctcagagcat tttctacagg     2940

ctgaacggcg tgggaatcac tcagcaggtc ctgtccgaga atcagaagct gattgccaac     3000

aagttcaacc aggccctggg agctatgcag accgggttta ccacaactaa cgaagctttc     3060

cgcaaagtgc aggacgcagt caacaataac gcacaggccc tgtccaagct ggcttctgag     3120

ctgagtaata cattcggagc aatctccgcc tctattgggg atatcattca gaggctggac     3180

gtgctggagc aggatgccca gatcgaccgg ctgattaatg gacgcctgac cacactgaac     3240

gcttttgtgg cacagcagct ggtccgaagt gaatcagcag ccctgtctgc ccagctggct     3300

aaggacaaag tgaacgagtg cgtcaaggct cagtcaaaac ggagcggctt ttgtgggcag     3360

ggcacccaca tcgtgagctt cgtggtcaat gcacctaacg gcctgtactt tatgcacgtg     3420

ggatactatc caagcaacca tatcgaggtg gtctccgctt atggcctgtg cgatgctgca     3480

aatcctacaa actgtattgc accagtgaac ggatacttca tcaaaactaa caacaccagg     3540

attgtggacg aatggtcata cactggctca agcttttatg cacccgagcc tatcacctcc     3600

ctgaacacaa agtacgtggc cccacatgtc acctatcaga atatctccac aaacctgcct     3660

ccacccctgc tgggcaattc taccggaatt gacttccagg atgaactgga cgagttcttt     3720

aagaatgtga gcacatccat ccccaacttt ggaagcctga ctcagattaa cactaccctg     3780

ctggatctga cctacgagat gctgagtctg cagcaggtgg tcaaggccct gaatgaatca     3840

tacatcgacc tgaaagagct ggggaattat acatactata acaagtggcc ctggtacatc     3900

tggctggggt tcattgcagg actggtggct ctggcactgt gcgtcttctt tatcctgtgc     3960

tgtactggat gcgggaccaa ctgtatgggc aagctgaaat gtaatcggtg ttgtgatcgc     4020

tacgaagaat acgacctgga gccccataaa gtgcatgtcc actaatga                  4068


<210>  2
<211>  1354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MERS-CoV Consensus Spike Antigen

<400>  2

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile 
            20                  25                  30          


Glu Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro 
        35                  40                  45              


Ile Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr 
    50                  55                  60                  


Tyr Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly 
65                  70                  75                  80  


Asp His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr 
                85                  90                  95      


Thr Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln 
            100                 105                 110         


Phe Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr 
        115                 120                 125             


Gly Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile 
    130                 135                 140                 


Tyr Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly 
145                 150                 155                 160 


Lys Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly 
                165                 170                 175     


Cys Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser 
            180                 185                 190         


Gly Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr 
        195                 200                 205             


His Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala 
    210                 215                 220                 


Ser Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe 
225                 230                 235                 240 


Met Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly 
                245                 250                 255     


Ile Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val 
            260                 265                 270         


Asp Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr 
        275                 280                 285             


Asp Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile 
    290                 295                 300                 


Gln Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln 
305                 310                 315                 320 


Pro Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg 
                325                 330                 335     


Ala Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr 
            340                 345                 350         


Glu Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu 
        355                 360                 365             


Ala Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys 
    370                 375                 380                 


Asp Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe 
385                 390                 395                 400 


Lys Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu 
                405                 410                 415     


Ser Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala 
            420                 425                 430         


Ala Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser 
        435                 440                 445             


Tyr Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro 
    450                 455                 460                 


Ile Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu 
465                 470                 475                 480 


Ile Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu 
                485                 490                 495     


Lys Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg 
            500                 505                 510         


Thr Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val 
        515                 520                 525             


Ser Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys 
    530                 535                 540                 


Gln Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser 
545                 550                 555                 560 


Thr Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val 
                565                 570                 575     


Gln Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala 
            580                 585                 590         


Asn Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser 
        595                 600                 605             


Leu Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val 
    610                 615                 620                 


Gly Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val 
625                 630                 635                 640 


Gly Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val 
                645                 650                 655     


Ser Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala 
            660                 665                 670         


Thr Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser 
        675                 680                 685             


Gln Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr 
    690                 695                 700                 


Tyr Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn 
705                 710                 715                 720 


Ser Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu 
                725                 730                 735     


Cys Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Arg Ser Val Arg 
            740                 745                 750         


Ser Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro 
        755                 760                 765             


Ile Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro 
    770                 775                 780                 


Thr Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile 
785                 790                 795                 800 


Gln Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln 
                805                 810                 815     


Lys Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile 
            820                 825                 830         


Asn Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg 
        835                 840                 845             


Asn Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro 
    850                 855                 860                 


Gly Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile 
865                 870                 875                 880 


Ser Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe 
                885                 890                 895     


Asp Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp 
            900                 905                 910         


Cys Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln 
        915                 920                 925             


Tyr Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met 
    930                 935                 940                 


Glu Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly 
945                 950                 955                 960 


Trp Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser 
                965                 970                 975     


Ile Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser 
            980                 985                 990         


Glu Asn Gln Lys Leu Ile Ala Asn  Lys Phe Asn Gln Ala  Leu Gly Ala 
        995                 1000                 1005             


Met Gln  Thr Gly Phe Thr Thr  Thr Asn Glu Ala Phe  Arg Lys Val 
    1010                 1015                 1020             


Gln Asp  Ala Val Asn Asn Asn  Ala Gln Ala Leu Ser  Lys Leu Ala 
    1025                 1030                 1035             


Ser Glu  Leu Ser Asn Thr Phe  Gly Ala Ile Ser Ala  Ser Ile Gly 
    1040                 1045                 1050             


Asp Ile  Ile Gln Arg Leu Asp  Val Leu Glu Gln Asp  Ala Gln Ile 
    1055                 1060                 1065             


Asp Arg  Leu Ile Asn Gly Arg  Leu Thr Thr Leu Asn  Ala Phe Val 
    1070                 1075                 1080             


Ala Gln  Gln Leu Val Arg Ser  Glu Ser Ala Ala Leu  Ser Ala Gln 
    1085                 1090                 1095             


Leu Ala  Lys Asp Lys Val Asn  Glu Cys Val Lys Ala  Gln Ser Lys 
    1100                 1105                 1110             


Arg Ser  Gly Phe Cys Gly Gln  Gly Thr His Ile Val  Ser Phe Val 
    1115                 1120                 1125             


Val Asn  Ala Pro Asn Gly Leu  Tyr Phe Met His Val  Gly Tyr Tyr 
    1130                 1135                 1140             


Pro Ser  Asn His Ile Glu Val  Val Ser Ala Tyr Gly  Leu Cys Asp 
    1145                 1150                 1155             


Ala Ala  Asn Pro Thr Asn Cys  Ile Ala Pro Val Asn  Gly Tyr Phe 
    1160                 1165                 1170             


Ile Lys  Thr Asn Asn Thr Arg  Ile Val Asp Glu Trp  Ser Tyr Thr 
    1175                 1180                 1185             


Gly Ser  Ser Phe Tyr Ala Pro  Glu Pro Ile Thr Ser  Leu Asn Thr 
    1190                 1195                 1200             


Lys Tyr  Val Ala Pro His Val  Thr Tyr Gln Asn Ile  Ser Thr Asn 
    1205                 1210                 1215             


Leu Pro  Pro Pro Leu Leu Gly  Asn Ser Thr Gly Ile  Asp Phe Gln 
    1220                 1225                 1230             


Asp Glu  Leu Asp Glu Phe Phe  Lys Asn Val Ser Thr  Ser Ile Pro 
    1235                 1240                 1245             


Asn Phe  Gly Ser Leu Thr Gln  Ile Asn Thr Thr Leu  Leu Asp Leu 
    1250                 1255                 1260             


Thr Tyr  Glu Met Leu Ser Leu  Gln Gln Val Val Lys  Ala Leu Asn 
    1265                 1270                 1275             


Glu Ser  Tyr Ile Asp Leu Lys  Glu Leu Gly Asn Tyr  Thr Tyr Tyr 
    1280                 1285                 1290             


Asn Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly Phe Ile  Ala Gly Leu 
    1295                 1300                 1305             


Val Ala  Leu Ala Leu Cys Val  Phe Phe Ile Leu Cys  Cys Thr Gly 
    1310                 1315                 1320             


Cys Gly  Thr Asn Cys Met Gly  Lys Leu Lys Cys Asn  Arg Cys Cys 
    1325                 1330                 1335             


Asp Arg  Tyr Glu Glu Tyr Asp  Leu Glu Pro His Lys  Val His Val 
    1340                 1345                 1350             


His 
    


<210>  3
<211>  4074
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MERS-CoV Consensus Spike Antigen lacking Cytoplasmic Domain

<400>  3
atggactgga cttggattct gttcctggtc gccgccgcaa ctcgcgtgca tagctacgtg       60

gatgtcggcc cagactctgt gaagagtgct tgcatcgagg tcgatattca gcagacattc      120

tttgacaaga cttggcctcg accaatcgac gtgagcaaag ccgacggcat catctacccc      180

cagggaagga cttatagtaa catcaccatt acataccagg gcctgttccc ttatcagggc      240

gaccacggag atatgtacgt gtattccgcc ggacatgcta ccgggaccac accacagaaa      300

ctgtttgtgg caaattattc tcaggacgtg aagcagttcg ccaacgggtt tgtggtcaga      360

atcggcgccg ctgcaaactc cactggcacc gtgatcattt ccccctctac cagtgccaca      420

atccggaaaa tctaccctgc ttttatgctg ggcagctccg tgggaaactt ctctgatggg      480

aagatgggcc gcttctttaa tcacaccctg gtgctgctgc cagacggatg cgggacactg      540

ctgagggcct tctactgtat cctggagccc agaagcggaa atcactgccc tgctgggaac      600

tcatacacca gctttgccac ttatcatacc cctgctacag actgttccga tggcaattat      660

aaccggaatg cctccctgaa ctctttcaag gaatacttta atctgcgcaa ctgcacattc      720

atgtacactt ataatatcac cgaggatgaa attctggagt ggttcgggat cacacagact      780

gctcagggcg tgcacctgtt ttctagtcgc tacgtcgatc tgtatggcgg aaacatgttc      840

cagtttgcca ccctgccagt gtacgacaca attaagtact atagcatcat tccccatagt      900

atccgatcaa ttcagagcga caggaaggct tgggccgctt tctacgtgta taaactgcag      960

cccctgacct tcctgctgga tttttctgtg gacggataca tcaggagagc cattgattgc     1020

gggtttaacg acctgagcca gctgcactgt tcctatgaat ctttcgatgt ggagtccggg     1080

gtgtactctg tctcaagctt tgaggctaag ccatcaggga gcgtggtcga gcaggcagaa     1140

ggcgtggagt gcgacttcag tcccctgctg tcaggcacac cccctcaggt gtacaatttc     1200

aaaagactgg tctttactaa ctgtaattac aacctgacca agctgctgag tctgttctca     1260

gtgaacgact ttacctgcag ccagatctcc cctgcagcca ttgccagcaa ttgttattcc     1320

tctctgatcc tggattactt ctcctatccc ctgtctatga aaagtgacct gtcagtgagt     1380

tcagcaggcc ctatctctca gtttaattac aagcagtcct tctctaaccc cacttgcctg     1440

attctggcca ccgtgcctca caacctgact accatcacaa agccactgaa atactcctat     1500

attaacaagt gcagcagact gctgtccgac gatcggactg aagtgcctca gctggtcaat     1560

gccaaccagt actctccatg cgtgagcatc gtcccctcaa ccgtgtggga agacggagat     1620

tactatcgga agcagctgag ccccctggag ggcggcggct ggctggtggc aagtgggtca     1680

acagtcgcca tgactgagca gctgcagatg ggcttcggaa tcaccgtgca gtacggcacc     1740

gatacaaatt ctgtctgtcc taagctggaa tttgctaacg acacaaaaat tgcaagtcag     1800

ctgggcaatt gcgtggagta ctctctgtat ggagtgagtg ggagaggcgt cttccagaac     1860

tgtacagccg tgggcgtccg acagcagagg ttcgtgtacg atgcttatca gaacctggtc     1920

ggctactatt ccgacgatgg aaattactat tgcctgcgag catgcgtgag cgtcccagtg     1980

tccgtcatct acgacaagga aactaaaacc cacgcaaccc tgttcggctc agtggcctgc     2040

gagcatatta gctccaccat gagccagtat agcagatcca cacggtccat gctgaaacgg     2100

cgcgactcta catacggacc cctgcagact cctgtggggt gcgtgctggg cctggtgaac     2160

tctagtctgt tcgtcgaaga ttgcaagctg ccactgggac agtctctgtg cgcactgcca     2220

gacacaccca gtacactgac tccacgcagc gtgcgatccg tcccaggaga gatgagactg     2280

gcaagcatcg ccttcaatca ccctattcag gtggatcagc tgaactcaag ctactttaag     2340

ctgtcaatcc caacaaactt cagctttggc gtgactcagg agtatatcca gacaactatt     2400

cagaaggtga ccgtcgactg caaacagtac gtgtgcaatg gattccagaa atgcgaacag     2460

ctgctgcggg agtatgggca gttttgttcc aagatcaatc aggcactgca tggcgccaac     2520

ctgcgccagg acgatagtgt gcgaaacctg ttcgcctcag tcaagtcctc tcagagttca     2580

cctatcattc cagggttcgg cggcgacttc aacctgaccc tgctggaacc cgtgagcatc     2640

agtaccggca gcaggagcgc cagaagcgca atcgaggatc tgctgtttga caaagtgacc     2700

attgccgacc caggatacat gcaggggtat gacgattgca tgcagcaggg accagcatcc     2760

gctcgcgatc tgatctgtgc tcagtacgtg gcagggtata aggtcctgcc acccctgatg     2820

gacgtgaaca tggaagctgc atatactagc tccctgctgg ggagcattgc aggagtggga     2880

tggaccgctg gactgtctag tttcgccgct atcccatttg ctcagagcat tttctacagg     2940

ctgaacggcg tgggaatcac tcagcaggtc ctgtccgaga atcagaagct gattgccaac     3000

aagttcaacc aggccctggg agctatgcag accgggttta ccacaactaa cgaagctttc     3060

cgcaaagtgc aggacgcagt caacaataac gcacaggccc tgtccaagct ggcttctgag     3120

ctgagtaata cattcggagc aatctccgcc tctattgggg atatcattca gaggctggac     3180

gtgctggagc aggatgccca gatcgaccgg ctgattaatg gacgcctgac cacactgaac     3240

gcttttgtgg cacagcagct ggtccgaagt gaatcagcag ccctgtctgc ccagctggct     3300

aaggacaaag tgaacgagtg cgtcaaggct cagtcaaaac ggagcggctt ttgtgggcag     3360

ggcacccaca tcgtgagctt cgtggtcaat gcacctaacg gcctgtactt tatgcacgtg     3420

ggatactatc caagcaacca tatcgaggtg gtctccgctt atggcctgtg cgatgctgca     3480

aatcctacaa actgtattgc accagtgaac ggatacttca tcaaaactaa caacaccagg     3540

attgtggacg aatggtcata cactggctca agcttttatg cacccgagcc tatcacctcc     3600

ctgaacacaa agtacgtggc cccacatgtc acctatcaga atatctccac aaacctgcct     3660

ccacccctgc tgggcaattc taccggaatt gacttccagg atgaactgga cgagttcttt     3720

aagaatgtga gcacatccat ccccaacttt ggaagcctga ctcagattaa cactaccctg     3780

ctggatctga cctacgagat gctgagtctg cagcaggtgg tcaaggccct gaatgaatca     3840

tacatcgacc tgaaagagct ggggaattat acatactata acaagtggcc ctggtacatc     3900

tggctggggt tcattgcagg actggtggct ctggcactgt gcgtcttctt tatctaatga     3960

ctgtgctgta ctggatgcgg gaccaactgt atgggcaagc tgaaatgtaa tcggtgttgt     4020

gatcgctacg aagaatacga cctggagccc cataaagtgc atgtccacta atga           4074


<210>  4
<211>  1318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MERS-CoV Consensus Spike Antigen lacking Cytoplasmic Domain

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Tyr Val Asp Val Gly Pro Asp Ser Val Lys Ser Ala Cys Ile 
            20                  25                  30          


Glu Val Asp Ile Gln Gln Thr Phe Phe Asp Lys Thr Trp Pro Arg Pro 
        35                  40                  45              


Ile Asp Val Ser Lys Ala Asp Gly Ile Ile Tyr Pro Gln Gly Arg Thr 
    50                  55                  60                  


Tyr Ser Asn Ile Thr Ile Thr Tyr Gln Gly Leu Phe Pro Tyr Gln Gly 
65                  70                  75                  80  


Asp His Gly Asp Met Tyr Val Tyr Ser Ala Gly His Ala Thr Gly Thr 
                85                  90                  95      


Thr Pro Gln Lys Leu Phe Val Ala Asn Tyr Ser Gln Asp Val Lys Gln 
            100                 105                 110         


Phe Ala Asn Gly Phe Val Val Arg Ile Gly Ala Ala Ala Asn Ser Thr 
        115                 120                 125             


Gly Thr Val Ile Ile Ser Pro Ser Thr Ser Ala Thr Ile Arg Lys Ile 
    130                 135                 140                 


Tyr Pro Ala Phe Met Leu Gly Ser Ser Val Gly Asn Phe Ser Asp Gly 
145                 150                 155                 160 


Lys Met Gly Arg Phe Phe Asn His Thr Leu Val Leu Leu Pro Asp Gly 
                165                 170                 175     


Cys Gly Thr Leu Leu Arg Ala Phe Tyr Cys Ile Leu Glu Pro Arg Ser 
            180                 185                 190         


Gly Asn His Cys Pro Ala Gly Asn Ser Tyr Thr Ser Phe Ala Thr Tyr 
        195                 200                 205             


His Thr Pro Ala Thr Asp Cys Ser Asp Gly Asn Tyr Asn Arg Asn Ala 
    210                 215                 220                 


Ser Leu Asn Ser Phe Lys Glu Tyr Phe Asn Leu Arg Asn Cys Thr Phe 
225                 230                 235                 240 


Met Tyr Thr Tyr Asn Ile Thr Glu Asp Glu Ile Leu Glu Trp Phe Gly 
                245                 250                 255     


Ile Thr Gln Thr Ala Gln Gly Val His Leu Phe Ser Ser Arg Tyr Val 
            260                 265                 270         


Asp Leu Tyr Gly Gly Asn Met Phe Gln Phe Ala Thr Leu Pro Val Tyr 
        275                 280                 285             


Asp Thr Ile Lys Tyr Tyr Ser Ile Ile Pro His Ser Ile Arg Ser Ile 
    290                 295                 300                 


Gln Ser Asp Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln 
305                 310                 315                 320 


Pro Leu Thr Phe Leu Leu Asp Phe Ser Val Asp Gly Tyr Ile Arg Arg 
                325                 330                 335     


Ala Ile Asp Cys Gly Phe Asn Asp Leu Ser Gln Leu His Cys Ser Tyr 
            340                 345                 350         


Glu Ser Phe Asp Val Glu Ser Gly Val Tyr Ser Val Ser Ser Phe Glu 
        355                 360                 365             


Ala Lys Pro Ser Gly Ser Val Val Glu Gln Ala Glu Gly Val Glu Cys 
    370                 375                 380                 


Asp Phe Ser Pro Leu Leu Ser Gly Thr Pro Pro Gln Val Tyr Asn Phe 
385                 390                 395                 400 


Lys Arg Leu Val Phe Thr Asn Cys Asn Tyr Asn Leu Thr Lys Leu Leu 
                405                 410                 415     


Ser Leu Phe Ser Val Asn Asp Phe Thr Cys Ser Gln Ile Ser Pro Ala 
            420                 425                 430         


Ala Ile Ala Ser Asn Cys Tyr Ser Ser Leu Ile Leu Asp Tyr Phe Ser 
        435                 440                 445             


Tyr Pro Leu Ser Met Lys Ser Asp Leu Ser Val Ser Ser Ala Gly Pro 
    450                 455                 460                 


Ile Ser Gln Phe Asn Tyr Lys Gln Ser Phe Ser Asn Pro Thr Cys Leu 
465                 470                 475                 480 


Ile Leu Ala Thr Val Pro His Asn Leu Thr Thr Ile Thr Lys Pro Leu 
                485                 490                 495     


Lys Tyr Ser Tyr Ile Asn Lys Cys Ser Arg Leu Leu Ser Asp Asp Arg 
            500                 505                 510         


Thr Glu Val Pro Gln Leu Val Asn Ala Asn Gln Tyr Ser Pro Cys Val 
        515                 520                 525             


Ser Ile Val Pro Ser Thr Val Trp Glu Asp Gly Asp Tyr Tyr Arg Lys 
    530                 535                 540                 


Gln Leu Ser Pro Leu Glu Gly Gly Gly Trp Leu Val Ala Ser Gly Ser 
545                 550                 555                 560 


Thr Val Ala Met Thr Glu Gln Leu Gln Met Gly Phe Gly Ile Thr Val 
                565                 570                 575     


Gln Tyr Gly Thr Asp Thr Asn Ser Val Cys Pro Lys Leu Glu Phe Ala 
            580                 585                 590         


Asn Asp Thr Lys Ile Ala Ser Gln Leu Gly Asn Cys Val Glu Tyr Ser 
        595                 600                 605             


Leu Tyr Gly Val Ser Gly Arg Gly Val Phe Gln Asn Cys Thr Ala Val 
    610                 615                 620                 


Gly Val Arg Gln Gln Arg Phe Val Tyr Asp Ala Tyr Gln Asn Leu Val 
625                 630                 635                 640 


Gly Tyr Tyr Ser Asp Asp Gly Asn Tyr Tyr Cys Leu Arg Ala Cys Val 
                645                 650                 655     


Ser Val Pro Val Ser Val Ile Tyr Asp Lys Glu Thr Lys Thr His Ala 
            660                 665                 670         


Thr Leu Phe Gly Ser Val Ala Cys Glu His Ile Ser Ser Thr Met Ser 
        675                 680                 685             


Gln Tyr Ser Arg Ser Thr Arg Ser Met Leu Lys Arg Arg Asp Ser Thr 
    690                 695                 700                 


Tyr Gly Pro Leu Gln Thr Pro Val Gly Cys Val Leu Gly Leu Val Asn 
705                 710                 715                 720 


Ser Ser Leu Phe Val Glu Asp Cys Lys Leu Pro Leu Gly Gln Ser Leu 
                725                 730                 735     


Cys Ala Leu Pro Asp Thr Pro Ser Thr Leu Thr Pro Arg Ser Val Arg 
            740                 745                 750         


Ser Val Pro Gly Glu Met Arg Leu Ala Ser Ile Ala Phe Asn His Pro 
        755                 760                 765             


Ile Gln Val Asp Gln Leu Asn Ser Ser Tyr Phe Lys Leu Ser Ile Pro 
    770                 775                 780                 


Thr Asn Phe Ser Phe Gly Val Thr Gln Glu Tyr Ile Gln Thr Thr Ile 
785                 790                 795                 800 


Gln Lys Val Thr Val Asp Cys Lys Gln Tyr Val Cys Asn Gly Phe Gln 
                805                 810                 815     


Lys Cys Glu Gln Leu Leu Arg Glu Tyr Gly Gln Phe Cys Ser Lys Ile 
            820                 825                 830         


Asn Gln Ala Leu His Gly Ala Asn Leu Arg Gln Asp Asp Ser Val Arg 
        835                 840                 845             


Asn Leu Phe Ala Ser Val Lys Ser Ser Gln Ser Ser Pro Ile Ile Pro 
    850                 855                 860                 


Gly Phe Gly Gly Asp Phe Asn Leu Thr Leu Leu Glu Pro Val Ser Ile 
865                 870                 875                 880 


Ser Thr Gly Ser Arg Ser Ala Arg Ser Ala Ile Glu Asp Leu Leu Phe 
                885                 890                 895     


Asp Lys Val Thr Ile Ala Asp Pro Gly Tyr Met Gln Gly Tyr Asp Asp 
            900                 905                 910         


Cys Met Gln Gln Gly Pro Ala Ser Ala Arg Asp Leu Ile Cys Ala Gln 
        915                 920                 925             


Tyr Val Ala Gly Tyr Lys Val Leu Pro Pro Leu Met Asp Val Asn Met 
    930                 935                 940                 


Glu Ala Ala Tyr Thr Ser Ser Leu Leu Gly Ser Ile Ala Gly Val Gly 
945                 950                 955                 960 


Trp Thr Ala Gly Leu Ser Ser Phe Ala Ala Ile Pro Phe Ala Gln Ser 
                965                 970                 975     


Ile Phe Tyr Arg Leu Asn Gly Val Gly Ile Thr Gln Gln Val Leu Ser 
            980                 985                 990         


Glu Asn Gln Lys Leu Ile Ala Asn  Lys Phe Asn Gln Ala  Leu Gly Ala 
        995                 1000                 1005             


Met Gln  Thr Gly Phe Thr Thr  Thr Asn Glu Ala Phe  Arg Lys Val 
    1010                 1015                 1020             


Gln Asp  Ala Val Asn Asn Asn  Ala Gln Ala Leu Ser  Lys Leu Ala 
    1025                 1030                 1035             


Ser Glu  Leu Ser Asn Thr Phe  Gly Ala Ile Ser Ala  Ser Ile Gly 
    1040                 1045                 1050             


Asp Ile  Ile Gln Arg Leu Asp  Val Leu Glu Gln Asp  Ala Gln Ile 
    1055                 1060                 1065             


Asp Arg  Leu Ile Asn Gly Arg  Leu Thr Thr Leu Asn  Ala Phe Val 
    1070                 1075                 1080             


Ala Gln  Gln Leu Val Arg Ser  Glu Ser Ala Ala Leu  Ser Ala Gln 
    1085                 1090                 1095             


Leu Ala  Lys Asp Lys Val Asn  Glu Cys Val Lys Ala  Gln Ser Lys 
    1100                 1105                 1110             


Arg Ser  Gly Phe Cys Gly Gln  Gly Thr His Ile Val  Ser Phe Val 
    1115                 1120                 1125             


Val Asn  Ala Pro Asn Gly Leu  Tyr Phe Met His Val  Gly Tyr Tyr 
    1130                 1135                 1140             


Pro Ser  Asn His Ile Glu Val  Val Ser Ala Tyr Gly  Leu Cys Asp 
    1145                 1150                 1155             


Ala Ala  Asn Pro Thr Asn Cys  Ile Ala Pro Val Asn  Gly Tyr Phe 
    1160                 1165                 1170             


Ile Lys  Thr Asn Asn Thr Arg  Ile Val Asp Glu Trp  Ser Tyr Thr 
    1175                 1180                 1185             


Gly Ser  Ser Phe Tyr Ala Pro  Glu Pro Ile Thr Ser  Leu Asn Thr 
    1190                 1195                 1200             


Lys Tyr  Val Ala Pro His Val  Thr Tyr Gln Asn Ile  Ser Thr Asn 
    1205                 1210                 1215             


Leu Pro  Pro Pro Leu Leu Gly  Asn Ser Thr Gly Ile  Asp Phe Gln 
    1220                 1225                 1230             


Asp Glu  Leu Asp Glu Phe Phe  Lys Asn Val Ser Thr  Ser Ile Pro 
    1235                 1240                 1245             


Asn Phe  Gly Ser Leu Thr Gln  Ile Asn Thr Thr Leu  Leu Asp Leu 
    1250                 1255                 1260             


Thr Tyr  Glu Met Leu Ser Leu  Gln Gln Val Val Lys  Ala Leu Asn 
    1265                 1270                 1275             


Glu Ser  Tyr Ile Asp Leu Lys  Glu Leu Gly Asn Tyr  Thr Tyr Tyr 
    1280                 1285                 1290             


Asn Lys  Trp Pro Trp Tyr Ile  Trp Leu Gly Phe Ile  Ala Gly Leu 
    1295                 1300                 1305             


Val Ala  Leu Ala Leu Cys Val  Phe Phe Ile 
    1310                 1315             


<210>  5
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgE leader sequence

<400>  5
atggactgga cttggattct gttcctggtc gccgccgcaa ctcgcgtgca tagc             54


<210>  6
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader sequence

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  7

Arg Lys Ala Trp Ala Ala Phe Tyr Val Tyr Lys Leu Gln Pro Leu 
1               5                   10                  15  


