<110>    National Cancer Center
         JP BIO A Inc.
         Kookmin University Industry Academy Cooperation Foundation

<120>    An humanized antibody to Digoxigenin and use thereof

<130>    FC20192WO

<150>    KR 10-2020-0109727
<151>    2020-08-28

<160>    40

<170>    KoPatentIn 3.0

<210>    1
<211>    121
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Mus musculus VH-m


<400>    1
Gln Val Thr Leu Lys Glu Ser Gly Pro Gly Ile Leu Gln Pro Ser Gln
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Ser Leu Ser Gly Phe Ser Leu Thr Thr Ser
             20                  25                  30 

Gly Met Gly Val Gly Trp Ile Arg Gln Ser Ser Gly Lys Gly Leu Glu
         35                  40                  45 

Trp Leu Ala Asn Ile Trp Trp Tyr Asp Thr Lys Tyr Tyr Asn Ala Ala
     50                  55                  60 

Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val
 65                  70                  75                  80 

Phe Leu Lys Ile Val Ser Val Asp Thr Ala Asp Thr Ala Thr Tyr Tyr
                 85                  90                  95 

Cys Gly Arg Ile His Tyr Asn Gly Ser Arg Phe Gly Asp Tyr Trp Gly
            100                 105                 110 

Gln Gly Thr Thr Leu Thr Val Ser Ser
        115                 120    


<210>    2
<211>    122
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VH-h1


<400>    2
Gln Val Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln
  1               5                  10                  15 

Thr Leu Thr Leu Thr Cys Thr Leu Ser Gly Phe Ser Leu Thr Thr Ser
             20                  25                  30 

Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu
         35                  40                  45 

Trp Val Leu Ala Asn Ile Trp Trp Tyr Asp Thr Lys Tyr Tyr Asn Ala
     50                  55                  60 

Ser Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln
 65                  70                  75                  80 

Val Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr
                 85                  90                  95 

Tyr Cys Gly Arg Ile His Tyr Asn Gly Ser Arg Phe Gly Asp Tyr Trp
            100                 105                 110 

Gly Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120        


<210>    3
<211>    121
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VH-h2


<400>    3
Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys Pro Thr Gln
  1               5                  10                  15 

Thr Leu Thr Leu Thr Cys Thr Leu Ser Gly Phe Ser Leu Thr Thr Ser
             20                  25                  30 

Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu
         35                  40                  45 

Trp Leu Ala Asn Leu Trp Trp Tyr Asp Thr Lys Tyr Tyr Asn Ala Ser
     50                  55                  60 

Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys Asn Gln Val
 65                  70                  75                  80 

Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Thr Tyr Tyr
                 85                  90                  95 

Cys Gly Arg Leu His Tyr Asn Gly Ser Arg Phe Gly Asp Tyr Trp Gly
            100                 105                 110 

Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120    


<210>    4
<211>    121
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VH-h3


<400>    4
Gln Val Thr Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Leu Ser Gly Phe Ser Leu Thr Thr Ser
             20                  25                  30 

Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu
         35                  40                  45 

Trp Leu Ala Asn Ile Trp Trp Tyr Asp Thr Lys Tyr Tyr Asn Ala Ser
     50                  55                  60 

Leu Lys Ser Arg Val Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val
 65                  70                  75                  80 

Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr
                 85                  90                  95 

Cys Gly Arg Ile His Tyr Asn Gly Ser Arg Phe Gly Asp Tyr Trp Gly
            100                 105                 110 

Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120    


<210>    5
<211>    121
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VH-h4


<400>    5
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu
  1               5                  10                  15 

Thr Leu Ser Leu Thr Cys Thr Leu Ser Gly Phe Ser Leu Thr Thr Ser
             20                  25                  30 

Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu
         35                  40                  45 

Trp Ile Ala Asn Ile Trp Trp Tyr Asp Thr Lys Tyr Tyr Asn Ala Ser
     50                  55                  60 

Leu Lys Ser Arg Val Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val
 65                  70                  75                  80 

Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr
                 85                  90                  95 

Cys Gly Arg Ile His Tyr Asn Gly Ser Arg Phe Gly Asp Tyr Trp Gly
            100                 105                 110 

Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120    


<210>    6
<211>    111
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Mus musculus VL-m


<400>    6
Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Phe Gly
  1               5                  10                  15 

Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Thr
             20                  25                  30 

Asn Gly Lys Thr Tyr Leu Met Trp Leu Leu Gln Arg Pro Gly Gln Ser
         35                  40                  45 

Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Val Pro
     50                  55                  60 

Gly Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
 65                  70                  75                  80 

Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Leu Thr Ile
                 85                  90                  95 

His Phe Pro Tyr Ser Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110 




<210>    7
<211>    112
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VL-h1


<400>    7
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly
  1               5                  10                  15 

Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Tyr Thr
             20                  25                  30 

Asn Gly Lys Thr Tyr Leu Met Trp Leu Gln Gln Arg Pro Gly Gln Ser
         35                  40                  45 

Pro Arg Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Val Pro
     50                  55                  60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
 65                  70                  75                  80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Leu Gln Thr
                 85                  90                  95 

Thr His Phe Pro Tyr Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110 




<210>    8
<211>    112
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VL-h2


<400>    8
Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly
  1               5                  10                  15 

Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu Tyr Thr
             20                  25                  30 

Asn Gly Lys Thr Tyr Leu Met Trp Leu Gln Gln Arg Pro Gly Gln Ser
         35                  40                  45 

Pro Arg Arg Leu Ile Tyr Leu Val Ser Thr Trp Asp Ser Gly Val Pro
     50                  55                  60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
 65                  70                  75                  80 

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Leu Gln Thr
                 85                  90                  95 

Thr His Phe Pro Tyr Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110 




<210>    9
<211>    112
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VL-h3


<400>    9
Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
  1               5                  10                  15 

Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Thr
             20                  25                  30 

Asn Gly Lys Thr Tyr Leu Met Trp Leu Gln Gln Lys Pro Gly Gln Pro
         35                  40                  45 

Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Val Pro
     50                  55                  60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
 65                  70                  75                  80 

Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Leu Gln Thr
                 85                  90                  95 

Thr His Phe Pro Tyr Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110 




<210>    10
<211>    112
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Humanized VL-h4

<400>    10
Asp Val Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
  1               5                  10                  15 

Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Thr
             20                  25                  30 

Asn Gly Lys Thr Tyr Leu Met Trp Leu Gln Gln Lys Pro Gly Gln Pro
         35                  40                  45 

Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Arg Asp Ser Gly Val Pro
     50                  55                  60 

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
 65                  70                  75                  80 

Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Leu Gln Thr
                 85                  90                  95 

Thr His Phe Pro Tyr Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110 




<210>    11
<211>    32
<212>    DNA_RNA
<213>    Artificial Sequence

<220>
<223>    internal 2'-O-methyl-modified aptamer SQ7-1(8)


<220>
<223>    internal 2'-O-methyl-modified aptamers (from 1to 4 and 29 to 32)


<220>
<221>    modified_base
<222>    (1)..(4)
<223>    2'-O-methyl-modified bases from 1 to 4


<220>
<221>    modified_base
<222>    (29)..(32)
<223>    2'-O-methyl-modified bases from 29 to 32


<400>    11
guuggtatat acttctttag cttggaacca ac                                       32



<210>    12
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ7


<400>    12
agcagcacag aggtcagatg atgttggtat atacttcttt agcttggaac caactcttgc         60

cctatgcgtg ctaccgtgaa                                                     80




<210>    13
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ7-1


<400>    13
gttggtatat acttctttag cttggaacca ac                                       32




<210>    14
<211>    32
<212>    DNA_RNA
<213>    Artificial Sequence

<220>
<223>    internal 2'-O-methyl-modified aptamer SQ7-1(1)

<220>
<223>    internal 2'-O-methyl-modified aptamers (from 1 to 6)


<400>    14
guugguatat acttctttag cttggaacca ac                                       32



<210> 15
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(2)

<220>
<223> internal 2'-O-methyl-modified aptamers (from 7 to 11)

<220>
<221> modified_base
<222> (7)..(11)
<223> 2'-O-methyl-modified bases from 7 to 11

<400> 15
gttggtauau acttctttag cttggaacca ac                                       32



<210> 16
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(3)

<220>
<223> internal 2'-O-methyl-modified aptamers (from 14 to 18)

<220>
<221> modified_base
<222> (14)..(18)
<223> 2'-O-methyl-modified bases from 14 to 18

<400> 16
gttggtatat actucuuuag cttggaacca ac                                       32



<210> 17
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(4)

<220>
<223> internal 2'-O-methyl-modified aptamer (from 21 to 26)

<220>
<221> modified_base
<222> (21)..(26)
<223> 2'-O-methyl-modified bases from 21 to 26

<400> 17
gttggtatat acttctttag cuuggaacca ac                                       32



<210> 18
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(5)

<220>
<223> internal 2'-O-methyl-modified aptamers (from 27 to 32)

<220>
<221> modified_base
<222> (27)..(32)
<223> 2'-O-methyl-modified bases from 27 to 32

<400> 18
gttggtatat acttctttag cttggaacca ac                                       32



<210> 19
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(6)

<220>
<223> internal 2'-O-methyl-modified aptamers (from 7 to 26)

<220>
<221> modified_base
<222> (7)..(26)
<223> 2'-O-methyl-modified bases from 7 to 26

<400> 19
gttggtauau acuucuuuag cuuggaacca ac                                       32



<210> 20
<211> 32
<212> DNA_RNA
<213> Artificial Sequence

<220>
<223> internal 2'-O-methyl-modified aptamer SQ7-1(1,5)

<220>
<223> internal 2'-O-methyl-modified aptamers (from 1 to 6 and 27 to 32)

<220>
<221> modified_base
<222> (1)..(6)
<223> 2'-O-methyl-modified bases from 1 to 6

<220>
<221> modified_base
<222> (27)..(32)
<223> 2'-O-methyl-modified bases from 27 to 32

<400> 20
guugguatat acttctttag cttggaacca ac                                       32


<210>    21
<211>    32
<212>    DNA_RNA
<213>    Artificial Sequence

<220>
<223>    internal 2'-O-methyl-modified aptamer SQ7-1(7)


<220>
<223>    Artificial Sequence


<220>
<221>    modified_base
<222>    (1)..(5)
<223>    2'-O-methyl-modified bases from 1 to 5


<220>
<221>    modified_base
<222>    (27)..(32)
<223>    2'-O-methyl-modified bases from 27 to 32


<400>    21
guuggtatat acttctttag cttggaacca ac                                       32


<210>    22
<211>    32
<212>    DNA_RNA
<213>    Artificial Sequence

<220>
<223>    internal 2'-O-methyl-modified aptamer SQ7-1(9)


<220>
<223>    Artificial Sequence


<220>
<221>    modified_base
<222>    (1)..(3)
<223>    2'-O-methyl-modified bases from 1 to 3


<220>
<221>    modified_base
<222>    (30)..(32)
<223>    2'-O-methyl-modified bases from 30 to 32


<400>    22
guuggtatat acttctttag cttggaacca ac                                       32



<210> 23
<211> 79
<212> DNA
<213> Artificial Sequence

<220>
<223> DNA aptamer SQ7a

<400> 23
agcagcacag aggtcagatg atgttggtat atacttcttt agcttggaac caactcttct 60

cctatgcgtg ctaccgtga                                                      79



<210> 24
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> DNA aptamer SQ7b

<400> 24
agcaacacag aggtcagatg atgttggtat atacttcttt agcttggaac ccactcttgt 60

cctatgcgtg ctaccgtgaa                                                      80



<210> 25
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> DNA aptamer SQ1

<400> 25
agcagcacag aggtcagatg cttgggctat ctcttattca tgctgttcca ccgctctcgg 60

cctatgcgtg ctaccgtgaa                                                      80



<210>    26
<211>    79
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ2


<400>    26
agcagcacag aggtcagatg aaccgcgatc tcatctgtac gctcacccgg tcgaagggac         60

ctatgcgtgc taccgtgaa                                                      79


<210>    27
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ3


<400>    27
agcagcacag aggtcagatg tgggggcgat tacgacccgg cgaaattaat agatctgccg         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    28
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ4


<400>    28
agcagcacag aggtcagatg agtgtccaac gtcggcgaaa ttaataggtt ggaacgaacg         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    29
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ5


<400>    29
agcagcacag aggtcagatg tcatggcaaa acgtccctac gctacgatta gttaggtcga         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    30
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ6


<400>    30
agcagcacag aggtcagatg gcttgccaca acgtgcgacg ctatgactaa ttcggcgacc         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    31
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8


<400>    31
agcagcacag aggtcagatg cttgggctat ttcttattca tgctgttcca ccgctctcgg         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    32
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ9


<400>    32
agcagcacag aggtcagatg attcaacttt gaataagtcc agtgacttct aacatagtgg         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    33
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ10


<400>    33
agcagcacag aggtcagatg ctcttgaagg gatacattgc ccttcgatgc ttgtctgatc         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    34
<211>    80
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ11


<400>    34
agcagcacag aggtcagatg gtgccaacct gaagtgactg agatatcaac cggtaggcct         60

cctatgcgtg ctaccgtgaa                                                     80


<210>    35
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-1


<400>    35
cagcacagag gtcagatgct tgggctattt cttattcatg ctg                           43


<210>    36
<211>    26
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-2


<400>    36
cagcacagag gtcagatgct tgggct                                              26


<210>    37
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-3


<400>    37
catgctgttc caccgctctc ggcctatgcg tg                                       32


<210>    38
<211>    28
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-4


<400>    38
agcagcacag aggtcagatg cttgggct                                            28


<210>    39
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-5


<400>    39
ctcggcctat gcgtgctacc gtg                                                 23


<210>    40
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA aptamer SQ8-6


<400>    40
caccgctctc ggcctatgcg tgctaccgtg                                          30

