                         SEQUENCE LISTING

<110>  Ramot at Tel-Aviv University Ltd.
       BAR-ILAN UNIVERSITY
       Eldar-Finkelman, Hagit
       SENDEROWITZ, Hanoch
 
<120>  SMALL MOLECULE MODULATORS OF GSK-3 ACTIVITY

<130>  88351

<150>  US 63/072,291
<151>  2020-08-31

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  433
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ser Gly Arg Pro Arg Thr Thr Ser Phe Ala Glu Ser Cys Lys Pro 
1               5                   10                  15      


Val Gln Gln Pro Ser Ala Phe Gly Ser Met Lys Val Ser Arg Asp Lys 
            20                  25                  30          


Asp Gly Ser Lys Val Thr Thr Val Val Ala Thr Pro Gly Gln Gly Pro 
        35                  40                  45              


Asp Arg Pro Gln Glu Val Ser Tyr Thr Asp Thr Lys Val Ile Gly Asn 
    50                  55                  60                  


Gly Ser Phe Gly Val Val Tyr Gln Ala Lys Leu Cys Asp Ser Gly Glu 
65                  70                  75                  80  


Leu Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Phe Lys Asn Arg 
                85                  90                  95      


Glu Leu Gln Ile Met Arg Lys Leu Asp His Cys Asn Ile Val Arg Leu 
            100                 105                 110         


Arg Tyr Phe Phe Tyr Ser Ser Gly Glu Lys Lys Asp Glu Val Tyr Leu 
        115                 120                 125             


Asn Leu Val Leu Asp Tyr Val Pro Glu Thr Val Tyr Arg Val Ala Arg 
    130                 135                 140                 


His Tyr Ser Arg Ala Lys Gln Thr Leu Pro Val Ile Tyr Val Lys Leu 
145                 150                 155                 160 


Tyr Met Tyr Gln Leu Phe Arg Ser Leu Ala Tyr Ile His Ser Phe Gly 
                165                 170                 175     


Ile Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Leu Asp Pro Asp 
            180                 185                 190         


Thr Ala Val Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Gln Leu Val 
        195                 200                 205             


Arg Gly Glu Pro Asn Val Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala 
    210                 215                 220                 


Pro Glu Leu Ile Phe Gly Ala Thr Asp Tyr Thr Ser Ser Ile Asp Val 
225                 230                 235                 240 


Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Ile 
                245                 250                 255     


Phe Pro Gly Asp Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val 
            260                 265                 270         


Leu Gly Thr Pro Thr Arg Glu Gln Ile Arg Glu Met Asn Pro Asn Tyr 
        275                 280                 285             


Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp Thr Lys Asp 
    290                 295                 300                 


Ser Ser Gly Thr Gly His Phe Thr Ser Gly Val Arg Val Phe Arg Pro 
305                 310                 315                 320 


Arg Thr Pro Pro Glu Ala Ile Ala Leu Cys Ser Arg Leu Leu Glu Tyr 
                325                 330                 335     


Thr Pro Thr Ala Arg Leu Thr Pro Leu Glu Ala Cys Ala His Ser Phe 
            340                 345                 350         


Phe Asp Glu Leu Arg Asp Pro Asn Val Lys Leu Pro Asn Gly Arg Asp 
        355                 360                 365             


Thr Pro Ala Leu Phe Asn Phe Thr Thr Gln Glu Leu Ser Ser Asn Pro 
    370                 375                 380                 


Pro Leu Ala Thr Ile Leu Ile Pro Pro His Ala Arg Ile Gln Ala Ala 
385                 390                 395                 400 


Ala Ser Thr Pro Thr Asn Ala Thr Ala Ala Ser Asp Ala Asn Thr Gly 
                405                 410                 415     


Asp Arg Gly Gln Thr Asn Asn Ala Ala Ser Ala Ser Ala Ser Asn Ser 
            420                 425                 430         


Thr 
    


<210>  2
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic peptide


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  PHOSPHORYLATION

<400>  2

Ser Xaa Xaa Xaa Ser 
1               5   


<210>  3
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  89-95 loop of GSK-3

<400>  3

Gln Asp Lys Arg Phe Lys Asn 
1               5           


<210>  4
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Inhibitor L803


<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  PHOSPHORYLATION

<400>  4

Lys Glu Ala Pro Pro Ala Pro Pro Gln Ser Pro 
1               5                   10      


<210>  5
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L803F peptide amino acid sequence


<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  PHOSPHORYLATION

<400>  5

Lys Glu Ala Pro Pro Ala Pro Pro Gln Ser Pro Phe 
1               5                   10          


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MYRISTIC ACID ATTACHED TO THE N-TERMINUS OF L803 (Seq ID NO: 4)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N- terminally myristoylated

<400>  6

Lys Glu Ala Pro Pro Ala Pro Pro Gln Ser Pro 
1               5                   10      


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MYRISTIC ACID ATTACHED TO THE N-TERMINUS OF L803F (Seq ID NO: 5)


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N- terminally myristoylated

<400>  7

Lys Glu Ala Pro Pro Ala Pro Pro Gln Ser Pro Phe 
1               5                   10          


<210>  8
<211>  433
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSK-3 beta, F93A mutant

<400>  8

Met Ser Gly Arg Pro Arg Thr Thr Ser Phe Ala Glu Ser Cys Lys Pro 
1               5                   10                  15      


Val Gln Gln Pro Ser Ala Phe Gly Ser Met Lys Val Ser Arg Asp Lys 
            20                  25                  30          


Asp Gly Ser Lys Val Thr Thr Val Val Ala Thr Pro Gly Gln Gly Pro 
        35                  40                  45              


Asp Arg Pro Gln Glu Val Ser Tyr Thr Asp Thr Lys Val Ile Gly Asn 
    50                  55                  60                  


Gly Ser Phe Gly Val Val Tyr Gln Ala Lys Leu Cys Asp Ser Gly Glu 
65                  70                  75                  80  


Leu Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Ala Lys Asn Arg 
                85                  90                  95      


Glu Leu Gln Ile Met Arg Lys Leu Asp His Cys Asn Ile Val Arg Leu 
            100                 105                 110         


Arg Tyr Phe Phe Tyr Ser Ser Gly Glu Lys Lys Asp Glu Val Tyr Leu 
        115                 120                 125             


Asn Leu Val Leu Asp Tyr Val Pro Glu Thr Val Tyr Arg Val Ala Arg 
    130                 135                 140                 


His Tyr Ser Arg Ala Lys Gln Thr Leu Pro Val Ile Tyr Val Lys Leu 
145                 150                 155                 160 


Tyr Met Tyr Gln Leu Phe Arg Ser Leu Ala Tyr Ile His Ser Phe Gly 
                165                 170                 175     


Ile Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Leu Asp Pro Asp 
            180                 185                 190         


Thr Ala Val Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Gln Leu Val 
        195                 200                 205             


Arg Gly Glu Pro Asn Val Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala 
    210                 215                 220                 


Pro Glu Leu Ile Phe Gly Ala Thr Asp Tyr Thr Ser Ser Ile Asp Val 
225                 230                 235                 240 


Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Ile 
                245                 250                 255     


Phe Pro Gly Asp Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val 
            260                 265                 270         


Leu Gly Thr Pro Thr Arg Glu Gln Ile Arg Glu Met Asn Pro Asn Tyr 
        275                 280                 285             


Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp Thr Lys Asp 
    290                 295                 300                 


Ser Ser Gly Thr Gly His Phe Thr Ser Gly Val Arg Val Phe Arg Pro 
305                 310                 315                 320 


Arg Thr Pro Pro Glu Ala Ile Ala Leu Cys Ser Arg Leu Leu Glu Tyr 
                325                 330                 335     


Thr Pro Thr Ala Arg Leu Thr Pro Leu Glu Ala Cys Ala His Ser Phe 
            340                 345                 350         


Phe Asp Glu Leu Arg Asp Pro Asn Val Lys Leu Pro Asn Gly Arg Asp 
        355                 360                 365             


Thr Pro Ala Leu Phe Asn Phe Thr Thr Gln Glu Leu Ser Ser Asn Pro 
    370                 375                 380                 


Pro Leu Ala Thr Ile Leu Ile Pro Pro His Ala Arg Ile Gln Ala Ala 
385                 390                 395                 400 


Ala Ser Thr Pro Thr Asn Ala Thr Ala Ala Ser Asp Ala Asn Thr Gly 
                405                 410                 415     


Asp Arg Gly Gln Thr Asn Asn Ala Ala Ser Ala Ser Ala Ser Asn Ser 
            420                 425                 430         


Thr 
    


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  L807mts amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N- terminally myristoylated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  PHOSPHORYLATION

<400>  9

Gly Lys Glu Ala Pro Pro Ser Pro Pro Pro Ser Pro 
1               5                   10          


