                         SEQUENCE LISTING

<110>  Pfizer Inc.
       Foley, Timothy Leyden
       Helal, Christopher John
 
<120>  METHODS OF PROTECTING RNA

<130>  PC072662

<150>  US 63/072,778
<151>  2020-08-31

<150>  US 63/227,098
<151>  2021-07-29

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  454
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from HCV IRES

<400>  1
gccagccccc gauugggggc gacacuccac cauagaucac uccccuguga ggaacuacug       60

ucuucacgca gaaagcgucu agccauggcg uuaguaugag ugucgugcag ccuccaggac      120

ccccccuccc gggagagcca uaguggucug cggaaccggu gaguacaccg gaauugccag      180

gacgaccggg uccuuucuug gaucaacccg cucaaugccu ggagauuugg gcgugccccc      240

gcgagacugc uagccgagua guguuggguc gcgaaaggcc uugugguacu gccugauagg      300

gugcuugcga gugccccggg aggucucgua gaccgugcac caugagcacg aauccuaaac      360

cucaaagaaa aaccaaacgu aacaccaacc gccgcccaca ggacgucuga ggcggccgcc      420

gccucagacg uccugugggu uuauugcauc ccgc                                  454


<210>  2
<211>  489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from HCV IRES, with 
       T7promoter and primer annealing site

<400>  2
ggatcctaat acgactcact atagccagcc cccgattggg ggcgacactc caccatagat       60

cactcccctg tgaggaacta ctgtcttcac gcagaaagcg tctagccatg gcgttagtat      120

gagtgtcgtg cagcctccag gaccccccct cccgggagag ccatagtggt ctgcggaacc      180

ggtgagtaca ccggaattgc caggacgacc gggtcctttc ttggatcaac ccgctcaatg      240

cctggagatt tgggcgtgcc cccgcgagac tgctagccga gtagtgttgg gtcgcgaaag      300

gccttgtggt actgcctgat agggtgcttg cgagtgcccc gggaggtctc gtagaccgtg      360

caccatgagc acgaatccta aacctcaaag aaaaaccaaa cgtaacacca accgccgccc      420

acaggacgtc tgaggcggcc gccgcctcag acgtcctgtg ggtttattgc atcccgcctc      480

gagtctaga                                                              489


<210>  3
<211>  574
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride FgenI

<400>  3
ucucucgcua ucucggaauc gaggggucug gccuacgcug cgauugcuuc uucagagcau       60

ccuucuucag ccuugugccc ucuacagugg cagccucaca caaacuauca acauggcuuc      120

cgaauccagg cucuaccaga ucuccggcga gaccaagucu caucugcuca aguuccgcuu      180

gacgacaucu cgggccagca aaccucaggc agugauuuau uugauugaua aaaacacuca      240

cgaaauccgc caagacgaug acaagacugu auacaccucc cucgacgaga uagccgacga      300

ccuccccgac agcaccccuc gauucaucuu ccucagcuau cccuugacga ugcccgaugg      360

acggcuaucc gugccuuaca ccaugaucua cuaccucccc aucaacugca augccgcaac      420

aaggaugcuc uacgccggcg caaaagagcu gauacgcaac acggccgagg ugaacaaggu      480

uaucgauaua gagucugcag aggaucugga agauauucca aagcaguuga gcggauagaa      540

auaauaauaa aagaaauaac auguucgcua ugcc                                  574


<210>  4
<211>  612
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride FgenI, with primer annealing sites

<400>  4
uccaccauga aucacuccuc ucucgcuauc ucggaaucga ggggucuggc cuacgcugcg       60

auugcuucuu cagagcaucc uucuucagcc uugugcccuc uacaguggca gccucacaca      120

aacuaucaac auggcuuccg aauccaggcu cuaccagauc uccggcgaga ccaagucuca      180

ucugcucaag uuccgcuuga cgacaucucg ggccagcaaa ccucaggcag ugauuuauuu      240

gauugauaaa aacacucacg aaauccgcca agacgaugac aagacuguau acaccucccu      300

cgacgagaua gccgacgacc uccccgacag caccccucga uucaucuucc ucagcuaucc      360

cuugacgaug cccgauggac ggcuauccgu gccuuacacc augaucuacu accuccccau      420

caacugcaau gccgcaacaa ggaugcucua cgccggcgca aaagagcuga uacgcaacac      480

ggccgaggug aacaagguua ucgauauaga gucugcagag gaucuggaag auauuccaaa      540

gcaguugagc ggauagaaau aauaauaaaa gaaauaacau guucgcuaug ccgaaaaacc      600

aaacguaaca cc                                                          612


<210>  5
<211>  612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride FgenI, with primer annealing sites

<400>  5
tccaccatga atcactcctc tctcgctatc tcggaatcga ggggtctggc ctacgctgcg       60

attgcttctt cagagcatcc ttcttcagcc ttgtgccctc tacagtggca gcctcacaca      120

aactatcaac atggcttccg aatccaggct ctaccagatc tccggcgaga ccaagtctca      180

tctgctcaag ttccgcttga cgacatctcg ggccagcaaa cctcaggcag tgatttattt      240

gattgataaa aacactcacg aaatccgcca agacgatgac aagactgtat acacctccct      300

cgacgagata gccgacgacc tccccgacag cacccctcga ttcatcttcc tcagctatcc      360

cttgacgatg cccgatggac ggctatccgt gccttacacc atgatctact acctccccat      420

caactgcaat gccgcaacaa ggatgctcta cgccggcgca aaagagctga tacgcaacac      480

ggccgaggtg aacaaggtta tcgatataga gtctgcagag gatctggaag atattccaaa      540

gcagttgagc ggatagaaat aataataaaa gaaataacat gttcgctatg ccgaaaaacc      600

aaacgtaaca cc                                                          612


<210>  6
<211>  1667
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride Chi18-4

<400>  6
uacgccagac aauagauuca gcucgaauaa ugaagccgau uggucacuga uuaacuuggu       60

guaucuacug gguagcaaag gaccgaucuu caccucgcau gaccguugaa uguggcgcaa      120

uucuaguacu ugaauggccu ggccaagcuu auagaacaau cgagacgaau ugaacaccac      180

uaucaaaauc caaauuaugc gguuuucaau cauacaugug gcuuuauggc uggccauggc      240

ggccgguuca uuuacagcua acgcuuacgg agcuguucga ugcgucaugu aucucacagg      300

gcaacacgug guagucccuu cagaaccuca ucucguggau uccauaaccc auuugauacu      360

ggcuuuuaug cgcucugaug ucuucaaugu ggacaaaacg ccugcugaau ucccgcucuu      420

cgcaucaguu gcugagacgc gcgagaaguu caacgcaaau accaagauca uggucgcaau      480

cggcgguugg ggagacgcag gauuugaaga agcugcgcgu gacgauucau cgagaaagcg      540

gugggcuggc cacgucaagg ccaugguuga ccagacagga gccgauggca uugacauuga      600

cugggaauau ccgggaggga accgugacga uuauaaacuc auuccgaacu cucagcggga      660

augggagauu caggcauucg ugcuccuucu ucaagaauug cguuccgccu ugggacaaac      720

gaaacugcuc ucaauugcgg ugccagcgcu ggaacgagau uugauggccu ucacaaacuc      780

gacuauuccg uccauugugg aucaaguuga cuuuaucagu guaaugacuu acgacaugau      840

gaaccgacgc gacaacguug ucaagcacca cagcggcgug gcugacuccu gggaagcaau      900

gaagcgauau auagaucgcg gcgucccucc gcacaagcug aacuuuggac uugguuacua      960

ugccaaaugg uucaugacug aacaaugcga uguacagcac ccauuaggau gccgcacuca     1020

acugcuagaa gaccccgcca auggagccga ucuuggcaag acugcagcuu uuagcuggca     1080

cgacgagguu cccgcagaaa uggccaauuc cuuuggcaaa gcccaugcuc auggccgcua     1140

cuaugaagau ggaagcuacg gcuauuggga cgaugaagag aagagauggu gguccuacga     1200

cacgccucuc accaucaaag ccaaagugcc ucggcuucuc ggcgagcugc agcugggcgg     1260

gguguuugcc ugggggcugg gcgaggacgc uccgcaguuu cugcaccuga aggcgacugu     1320

ugacggcauu cggguucugc gcggagauga cgaugcugug aaggaugagc uguaacaagc     1380

agcggcgugu guacgucugg aguuuggcga caucauugaa ggcgugggau gcaauucaag     1440

ccauugauac ugcagauaag uugaagcagc aacagccaaa acucugcacg uggcccgaga     1500

cuuccgucuu auagcaggga acagacagga uggugcugac ggaauaugca gagcucgagc     1560

ucaaccggua ggcuagcuug aaaaccccau gagcuagcag uuaaccuugc aguuaagggu     1620

aaccagagcu uaaacacguc ugauuuggug caaugaauac aggcugc                   1667


<210>  7
<211>  1705
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride Chi18-4, with primer annealing sites

<400>  7
uccaccauga aucacuccua cgccagacaa uagauucagc ucgaauaaug aagccgauug       60

gucacugauu aacuuggugu aucuacuggg uagcaaagga ccgaucuuca ccucgcauga      120

ccguugaaug uggcgcaauu cuaguacuug aauggccugg ccaagcuuau agaacaaucg      180

agacgaauug aacaccacua ucaaaaucca aauuaugcgg uuuucaauca uacauguggc      240

uuuauggcug gccauggcgg ccgguucauu uacagcuaac gcuuacggag cuguucgaug      300

cgucauguau cucacagggc aacacguggu agucccuuca gaaccucauc ucguggauuc      360

cauaacccau uugauacugg cuuuuaugcg cucugauguc uucaaugugg acaaaacgcc      420

ugcugaauuc ccgcucuucg caucaguugc ugagacgcgc gagaaguuca acgcaaauac      480

caagaucaug gucgcaaucg gcgguugggg agacgcagga uuugaagaag cugcgcguga      540

cgauucaucg agaaagcggu gggcuggcca cgucaaggcc augguugacc agacaggagc      600

cgauggcauu gacauugacu gggaauaucc gggagggaac cgugacgauu auaaacucau      660

uccgaacucu cagcgggaau gggagauuca ggcauucgug cuccuucuuc aagaauugcg      720

uuccgccuug ggacaaacga aacugcucuc aauugcggug ccagcgcugg aacgagauuu      780

gauggccuuc acaaacucga cuauuccguc cauuguggau caaguugacu uuaucagugu      840

aaugacuuac gacaugauga accgacgcga caacguuguc aagcaccaca gcggcguggc      900

ugacuccugg gaagcaauga agcgauauau agaucgcggc gucccuccgc acaagcugaa      960

cuuuggacuu gguuacuaug ccaaaugguu caugacugaa caaugcgaug uacagcaccc     1020

auuaggaugc cgcacucaac ugcuagaaga ccccgccaau ggagccgauc uuggcaagac     1080

ugcagcuuuu agcuggcacg acgagguucc cgcagaaaug gccaauuccu uuggcaaagc     1140

ccaugcucau ggccgcuacu augaagaugg aagcuacggc uauugggacg augaagagaa     1200

gagauggugg uccuacgaca cgccucucac caucaaagcc aaagugccuc ggcuucucgg     1260

cgagcugcag cugggcgggg uguuugccug ggggcugggc gaggacgcuc cgcaguuucu     1320

gcaccugaag gcgacuguug acggcauucg gguucugcgc ggagaugacg augcugugaa     1380

ggaugagcug uaacaagcag cggcgugugu acgucuggag uuuggcgaca ucauugaagg     1440

cgugggaugc aauucaagcc auugauacug cagauaaguu gaagcagcaa cagccaaaac     1500

ucugcacgug gcccgagacu uccgucuuau agcagggaac agacaggaug gugcugacgg     1560

aauaugcaga gcucgagcuc aaccgguagg cuagcuugaa aaccccauga gcuagcaguu     1620

aaccuugcag uuaaggguaa ccagagcuua aacacgucug auuuggugca augaauacag     1680

gcugcgaaaa accaaacgua acacc                                           1705


<210>  8
<211>  1705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence: derived from Trichoderma 
       atroviride Chi18-4, with primer annealing sites

<400>  8
tccaccatga atcactccta cgccagacaa tagattcagc tcgaataatg aagccgattg       60

gtcactgatt aacttggtgt atctactggg tagcaaagga ccgatcttca cctcgcatga      120

ccgttgaatg tggcgcaatt ctagtacttg aatggcctgg ccaagcttat agaacaatcg      180

agacgaattg aacaccacta tcaaaatcca aattatgcgg ttttcaatca tacatgtggc      240

tttatggctg gccatggcgg ccggttcatt tacagctaac gcttacggag ctgttcgatg      300

cgtcatgtat ctcacagggc aacacgtggt agtcccttca gaacctcatc tcgtggattc      360

cataacccat ttgatactgg cttttatgcg ctctgatgtc ttcaatgtgg acaaaacgcc      420

tgctgaattc ccgctcttcg catcagttgc tgagacgcgc gagaagttca acgcaaatac      480

caagatcatg gtcgcaatcg gcggttgggg agacgcagga tttgaagaag ctgcgcgtga      540

cgattcatcg agaaagcggt gggctggcca cgtcaaggcc atggttgacc agacaggagc      600

cgatggcatt gacattgact gggaatatcc gggagggaac cgtgacgatt ataaactcat      660

tccgaactct cagcgggaat gggagattca ggcattcgtg ctccttcttc aagaattgcg      720

ttccgccttg ggacaaacga aactgctctc aattgcggtg ccagcgctgg aacgagattt      780

gatggccttc acaaactcga ctattccgtc cattgtggat caagttgact ttatcagtgt      840

aatgacttac gacatgatga accgacgcga caacgttgtc aagcaccaca gcggcgtggc      900

tgactcctgg gaagcaatga agcgatatat agatcgcggc gtccctccgc acaagctgaa      960

ctttggactt ggttactatg ccaaatggtt catgactgaa caatgcgatg tacagcaccc     1020

attaggatgc cgcactcaac tgctagaaga ccccgccaat ggagccgatc ttggcaagac     1080

tgcagctttt agctggcacg acgaggttcc cgcagaaatg gccaattcct ttggcaaagc     1140

ccatgctcat ggccgctact atgaagatgg aagctacggc tattgggacg atgaagagaa     1200

gagatggtgg tcctacgaca cgcctctcac catcaaagcc aaagtgcctc ggcttctcgg     1260

cgagctgcag ctgggcgggg tgtttgcctg ggggctgggc gaggacgctc cgcagtttct     1320

gcacctgaag gcgactgttg acggcattcg ggttctgcgc ggagatgacg atgctgtgaa     1380

ggatgagctg taacaagcag cggcgtgtgt acgtctggag tttggcgaca tcattgaagg     1440

cgtgggatgc aattcaagcc attgatactg cagataagtt gaagcagcaa cagccaaaac     1500

tctgcacgtg gcccgagact tccgtcttat agcagggaac agacaggatg gtgctgacgg     1560

aatatgcaga gctcgagctc aaccggtagg ctagcttgaa aaccccatga gctagcagtt     1620

aaccttgcag ttaagggtaa ccagagctta aacacgtctg atttggtgca atgaatacag     1680

gctgcgaaaa accaaacgta acacc                                           1705


<210>  9
<211>  30
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence

<400>  9
ggaaucucuc ucacgaacug acguaaucuu                                        30


