                         SEQUENCE LISTING

<110>  Charit - Universittsmedizin Berlin
 
<120>  A CHIMERIC ANTIGEN RECEPTOR CONSTRUCT ENCODING A CHECKPOINT 
       INHIBITORY MOLECULE AND AN IMMUNE STIMULATORY CYTOKINE AND CAR 
       EXPRESSING CELLS RECOGNIZING CD44v6

<130>  XII 2208/20WO

<150>  EP20192399.2
<151>  2020-08-24

<150>  EP20205696.6
<151>  2020-11-04

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  1
atgggatgga gctgtatcat cctcttcctg gtagcaacaa cagccaccgg cgtccatagc       60


<210>  2
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR variable

<400>  2
gagatcgtcc tgactcagag ccctgcaacc ctgtccctgt ctccaggcga acgggctaca       60

ctgagctgct ctgcctctag cagcattaac tacatttact ggtatcagca gaagccaggc      120

caagccccga ggctccttat ttacttgact tccaacctgg ctagtggagt gccggctagg      180

ttcagcggga gcggctcagg cacagacttc acattgacaa tatcaagcct ggaacccgaa      240

gattttgctg tgtactattg tctccaatgg tcttctaatc ctctcacgtt cggagggggg      300

actaaggtgg agattaaggg gtcaacctct ggcagcggga aacctggctc tggcgagggc      360

agcaccaagg gtcaagtcca gctccaagaa gtgcaactcg tggaatcagg aggcggcttg      420

gtgaagcccg gcggctcctt gagactgtct tgcgctgcta gcgggtttac attctcatct      480

tacgacatga gttgggttcg ccaggcacct ggaaaaggcc tcgagtgggt cagcactatt      540

tctagcggcg gatcttacac ctactatctg gacagcatca aaggacgctt cacaatttct      600

cgcgacaatg ctaagaactc tttgtatctc cagatgaact cccttagagc cgaggatact      660

gctgtctact attgcgctcg ccagggactc gattactggg gacggggcac actcgtgact      720

gtctcctctg tgcggccgcc gagc                                             744


<210>  3
<211>  693
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  3
cccaaatctt gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg       60

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc      120

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac      180

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac      240

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc      300

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc      360

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag      420

gaaatgacca aaaaccaggt cagcctgacc tgcctggtca aaggccttct atccagcgac      480

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc      540

gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg      600

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac      660

acgcagaaga gcctctccct gtctccgggt aag                                   693


<210>  4
<211>  222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  4
gatcccaaac tctgctactt ttgggtgctg gtggtggttg gtggagtcct ggcttgctat       60

agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc      120

ctgcacagtg actacatgaa catgactccc cgccgccccg ggcccacccg caagcattac      180

cagccctatg ccccaccacg cgacttcgca gcctatcgct cc                         222


<210>  5
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  5
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgcag agaaggaaga accctcagga aggcctgtac      180

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag      240

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac      300

acctacgacg cccttcatat gcaggctctg ccacctagc                             339


<210>  6
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  6
ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct       60

ggaccg                                                                  66


<210>  7
<211>  597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  7
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtt gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg gttggtgtcg tgggcggcct gctgggcagc      540

ctggtgctgc tagtctgggt cctggccgtc atctgctccc gggccgcacg agggtga         597


<210>  8
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  8
taatcccagt gtggtggtac ggaattctct agactgccgg atccaagctg gaggaaaaac       60

tgtttcatac agaaggcgtg gaggaaaaac tgtttcatac agaaggcgtg gaggaaaaac      120

tgtttcatac agaaggcgtg gaggaaaaac tgtttcatac agaaggcgtc gcgaattcgc      180

ggagactcta gagggtatat aatggaagct cgatttccag cttggcattc cggtactgtt      240

ggtaaacacc aagcttcacc                                                  260


<210>  9
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  9
atggtgagca agggcgagga gctgttcacc ggggtggtga tggcaccaag aagggctcgc       60

ggatgccgga ccctcggtct cccggcgctg cttctgctgc tgctgctccg gccgccggcg      120

acgcggggc                                                              129


<210>  10
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  10
atcacgtgtc ctccacccat gtccgtggaa cacgcagaca tctgggtcaa gagctacagc       60

ttgtactcca gggagcggta catttgtaac tctggtttca agaggaaagc cggcacgtcc      120

agcctgacgg agtgcgtgtt gaacaaggcc acgaatgtcg cccactggac aacccccagt      180

ctcaaatgca ttagagaccc tgccctggtt caccaaaggc cagcgccacc c               231


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  11
agcggcggag gatgtggagg cggaggctgt ggcggaagcg gaggcggagg cagt             54


<210>  12
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  12
atccagaact gggtgaatgt cataagtgat ctgaagaaaa ttgaagatct tattcagtct       60

atgcatattg atgctactct atatactgaa agtgatgttc accccagttg caaagtgaca      120

gcaatgaagt gctttctctt ggagctgcaa gttatttcac ttgagtccgg agatgcaagt      180

attcatgata cagtggaaaa tctgatcatc cttgcaaacg acagtttgtc ttctaatggg      240

aatgtaacag aatctggatg caaagaatgt gaggaactgg aggaaaaaaa tattaaagaa      300

tttttgcaga gttttgtaca tattgtccaa atgttcatca acacttcttg ataa            354


<210>  13
<211>  3749
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  13
atgggatgga gctgtatcat cctcttcctg gtagcaacaa cagccaccgg cgtccatagc       60

gagatcgtcc tgactcagag ccctgcaacc ctgtccctgt ctccaggcga acgggctaca      120

ctgagctgct ctgcctctag cagcattaac tacatttact ggtatcagca gaagccaggc      180

caagccccga ggctccttat ttacttgact tccaacctgg ctagtggagt gccggctagg      240

ttcagcggga gcggctcagg cacagacttc acattgacaa tatcaagcct ggaacccgaa      300

gattttgctg tgtactattg tctccaatgg tcttctaatc ctctcacgtt cggagggggg      360

actaaggtgg agattaaggg gtcaacctct ggcagcggga aacctggctc tggcgagggc      420

agcaccaagg gtcaagtcca gctccaagaa gtgcaactcg tggaatcagg aggcggcttg      480

gtgaagcccg gcggctcctt gagactgtct tgcgctgcta gcgggtttac attctcatct      540

tacgacatga gttgggttcg ccaggcacct ggaaaaggcc tcgagtgggt cagcactatt      600

tctagcggcg gatcttacac ctactatctg gacagcatca aaggacgctt cacaatttct      660

cgcgacaatg ctaagaactc tttgtatctc cagatgaact cccttagagc cgaggatact      720

gctgtctact attgcgctcg ccagggactc gattactggg gacggggcac actcgtgact      780

gtctcctctg tgcggccgcc gagccccaaa tcttgtgaca aaactcacac atgcccaccg      840

tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag      900

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac      960

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     1020

acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc     1080

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     1140

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     1200

tacaccctgc ccccatcccg ggaggaaatg accaaaaacc aggtcagcct gacctgcctg     1260

gtcaaaggcc ttctatccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     1320

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc     1380

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     1440

catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaggat     1500

cccaaactct gctacttttg ggtgctggtg gtggttggtg gagtcctggc ttgctatagc     1560

ttgctagtaa cagtggcctt tattattttc tgggtgagga gtaagaggag caggctcctg     1620

cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa gcattaccag     1680

ccctatgccc caccacgcga cttcgcagcc tatcgctcca gagtgaagtt cagcaggagc     1740

gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga     1800

cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga     1860

aagccgcaga gaaggaagaa ccctcaggaa ggcctgtaca atgaactgca gaaagataag     1920

atggcggagg cctacagtga gattgggatg aaaggcgagc gccggagggg caaggggcac     1980

gatggccttt accagggtct cagtacagcc accaaggaca cctacgacgc ccttcatatg     2040

caggctctgc cacctagcgg atccggcgca acaaacttct ctctgctgaa acaagccgga     2100

gatgtcgaag agaatcctgg accgatgcag atcccacagg cgccctggcc agtcgtctgg     2160

gcggtgctac aactgggctg gcggccagga tggttcttag actccccaga caggccctgg     2220

aaccccccca ccttctcccc agccctgctc gtggtgaccg aaggggacaa cgccaccttc     2280

acctgcagct tctccaacac atcggagagc ttcgtgctaa actggtaccg catgagcccc     2340

agcaaccaga cggacaagct ggccgccttc cccgaggacc gcagccagcc cggccaggac     2400

tgccgcttcc gtgtcacaca actgcccaac gggcgtgact tccacatgag cgttgtcagg     2460

gcccggcgca atgacagcgg cacctacctc tgtggggcca tctccctggc ccccaaggcg     2520

cagatcaaag agagcctgcg ggcagagctc agggtgacag agagaagggc agaagtgccc     2580

acagcccacc ccagcccctc acccaggcca gccggccagt tccaaaccct ggtggttggt     2640

gtcgtgggcg gcctgctggg cagcctggtg ctgctagtct gggtcctggc cgtcatctgc     2700

tcccgggccg cacgagggtg ataatcccag tgtggtggta cggaattctc tagactgccg     2760

gatccaagct ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata     2820

cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata     2880

cagaaggcgt cgcgaattcg cggagactct agagggtata taatggaagc tcgatttcca     2940

gcttggcatt ccggtactgt tggtaaacac caagcttcac catggtgagc aagggcgagg     3000

agctgttcac cggggtggtg atggcaccaa gaagggctcg cggatgccgg accctcggtc     3060

tcccggcgct gcttctgctg ctgctgctcc ggccgccggc gacgcggggc atcacgtgtc     3120

ctccacccat gtccgtggaa cacgcagaca tctgggtcaa gagctacagc ttgtactcca     3180

gggagcggta catttgtaac tctggtttca agaggaaagc cggcacgtcc agcctgacgg     3240

agtgcgtgtt gaacaaggcc acgaatgtcg cccactggac aacccccagt ctcaaatgca     3300

ttagagaccc tgccctggtt caccaaaggc cagcgccacc cagcggcgga ggatgtggag     3360

gcggaggctg tggcggaagc ggaggcggag gcagtatcca gaactgggtg aatgtcataa     3420

gtgatctgaa gaaaattgaa gatcttattc agtctatgca tattgatgct actctatata     3480

ctgaaagtga tgttcacccc agttgcaaag tgacagcaat gaagtgcttt ctcttggagc     3540

tgcaagttat ttcacttgag tccggagatg caagtattca tgatacagtg gaaaatctga     3600

tcatccttgc aaacgacagt ttgtcttcta atgggaatgt aacagaatct ggatgcaaag     3660

aatgtgagga actggaggaa aaaaatatta aagaattttt gcagagtttt gtacatattg     3720

tccaaatgtt catcaacact tcttgataa                                       3749


<210>  14
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  14

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Thr Ala Thr 
1               5                   10                  15      


Gly Val His Ser 
            20  


<210>  15
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  15

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Ile Asn Tyr Ile 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu 
65                  70                  75                  80  


Asp Phe Ala Val Tyr Tyr Cys Leu Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Ser Thr Ser Gly Ser 
            100                 105                 110         


Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val Gln Leu 
        115                 120                 125             


Gln 
    


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  16

Ser Ser Ile Asn Tyr Ile Tyr 
1               5           


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  17

Leu Leu Ile Tyr Leu Thr Ser Asn Leu Ala Ser 
1               5                   10      


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  18

Leu Gln Trp Ser Ser Asn Pro Leu 
1               5               


<210>  19
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  19

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr Tyr Leu Asp Ser Ile 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Gly Leu Asp Tyr Trp Gly Arg Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser Val Arg Pro Pro Ser 
        115                 


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  20

Phe Thr Phe Ser Ser Tyr Asp Met Ser 
1               5                   


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  21

Trp Val Ser Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr Tyr Leu 
1               5                   10                  15  


<210>  22
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  22

Arg Gln Gly Leu Asp Tyr 
1               5       


<210>  23
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  23

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Leu Leu Ser Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  24
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  24

Asp Pro Lys Leu Cys Tyr Phe Trp Val Leu Val Val Val Gly Gly Val 
1               5                   10                  15      


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            20                  25                  30          


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
        35                  40                  45              


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    50                  55                  60                  


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
65                  70                  


<210>  25
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  25

Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
1               5                   10                  15      


Val Thr Val Ala Phe Ile Ile 
            20              


<210>  26
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  26

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Ser 
    


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  27

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  28
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  28

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly 
        195             


<210>  29
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  29

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Met Ala Pro 
1               5                   10                  15      


Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala Leu Leu Leu 
            20                  25                  30          


Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg 
        35                  40          


<210>  30
<211>  78
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  30

Gly Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp 
1               5                   10                  15      


Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser 
            20                  25                  30          


Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu 
        35                  40                  45              


Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys 
    50                  55                  60                  


Ile Arg Asp Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro 
65                  70                  75              


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  31

Ser Gly Gly Gly Cys Gly Gly Gly Gly Cys Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser 
        


<210>  32
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  32

Ile Gln Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp 
1               5                   10                  15      


Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp 
            20                  25                  30          


Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu 
        35                  40                  45              


Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr 
    50                  55                  60                  


Val Glu Asn Leu Ile Ile Leu Ala Asn Asp Ser Leu Ser Ser Asn Gly 
65                  70                  75                  80  


Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys 
                85                  90                  95      


Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe 
            100                 105                 110         


Ile Asn Thr Ser 
        115     


<210>  33
<211>  686
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  33

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Thr Ala Thr 
1               5                   10                  15      


Gly Val His Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            20                  25                  30          


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser 
        35                  40                  45              


Ile Asn Tyr Ile Tyr Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg 
    50                  55                  60                  


Leu Leu Ile Tyr Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      


Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Leu Gln Trp Ser Ser 
            100                 105                 110         


Asn Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Ser 
        115                 120                 125             


Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly 
    130                 135                 140                 


Gln Val Gln Leu Gln Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
                165                 170                 175     


Thr Phe Ser Ser Tyr Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
            180                 185                 190         


Gly Leu Glu Trp Val Ser Thr Ile Ser Ser Gly Gly Ser Tyr Thr Tyr 
        195                 200                 205             


Tyr Leu Asp Ser Ile Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
    210                 215                 220                 


Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
225                 230                 235                 240 


Ala Val Tyr Tyr Cys Ala Arg Gln Gly Leu Asp Tyr Trp Gly Arg Gly 
                245                 250                 255     


Thr Leu Val Thr Val Ser Ser Val Arg Pro Pro Ser Pro Lys Ser Cys 
            260                 265                 270         


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
        275                 280                 285             


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
    290                 295                 300                 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
305                 310                 315                 320 


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
                325                 330                 335     


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
            340                 345                 350         


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
        355                 360                 365             


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
    370                 375                 380                 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
385                 390                 395                 400 


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
                405                 410                 415     


Leu Thr Cys Leu Val Lys Gly Leu Leu Ser Ser Asp Ile Ala Val Glu 
            420                 425                 430         


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
        435                 440                 445             


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
    450                 455                 460                 


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
465                 470                 475                 480 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
                485                 490                 495     


Pro Gly Lys Asp Pro Lys Leu Cys Tyr Phe Trp Val Leu Val Val Val 
            500                 505                 510         


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
        515                 520                 525             


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
    530                 535                 540                 


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
545                 550                 555                 560 


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
                565                 570                 575     


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
            580                 585                 590         


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
        595                 600                 605             


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg 
    610                 615                 620                 


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
625                 630                 635                 640 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
                645                 650                 655     


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
            660                 665                 670         


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Ser 
        675                 680                 685     


<210>  34
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  34

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly 
        195             


<210>  35
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  35

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Met Ala Pro 
1               5                   10                  15      


Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala Leu Leu Leu 
            20                  25                  30          


Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr Cys Pro Pro 
        35                  40                  45              


Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser Tyr Ser Leu 
    50                  55                  60                  


Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys Arg Lys Ala 
65                  70                  75                  80  


Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala Thr Asn Val 
                85                  90                  95      


Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp Pro Ala Leu 
            100                 105                 110         


Val His Gln Arg Pro Ala Pro Pro Ser Gly Gly Gly Cys Gly Gly Gly 
        115                 120                 125             


Gly Cys Gly Gly Ser Gly Gly Gly Gly Ser Ile Gln Asn Trp Val Asn 
    130                 135                 140                 


Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His 
145                 150                 155                 160 


Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys 
                165                 170                 175     


Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu 
            180                 185                 190         


Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile 
        195                 200                 205             


Leu Ala Asn Asp Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly 
    210                 215                 220                 


Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu 
225                 230                 235                 240 


Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
                245                 250                 


<210>  36
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  36

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Thr Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Thr Phe 
                85                  90                  95      


Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Ser Thr Ser Gly Ser Gly 
            100                 105                 110         


Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val Gln Leu Gln 
        115                 120                 125             


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  37

Ser Ser Val Ser Tyr Met His 
1               5           


<210>  38
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  38

Leu Leu Ile Tyr Ser Thr Ser Asn Leu Ala Ser 
1               5                   10      


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  39

His Gln Trp Ser Ser Tyr Pro 
1               5           


<210>  40
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  40

Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr 
1               5                   10                  15      


Cys Thr Val Ser Gly Phe Thr Ile Ser Ser Gly Tyr Ser Trp His Trp 
            20                  25                  30          


Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile Gly Tyr Ile Gln 
        35                  40                  45              


Tyr Ser Gly Ile Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr 
    50                  55                  60                  


Met Leu Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu Ser Ser 
65                  70                  75                  80  


Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Asp Tyr 
                85                  90                  95      


Asp Tyr His Trp Tyr Phe Asp Val Trp Gly Gln Gly Ser Thr Val Thr 
            100                 105                 110         


Val Ser Ser Gly Ala Ala Ala Glu 
        115                 120 


<210>  41
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  41

Phe Thr Ile Ser Ser Gly Tyr Ser Trp His 
1               5                   10  


<210>  42
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  42

Trp Ile Gly Tyr Ile Gln Tyr Ser Gly Ile Thr Asn Tyr 
1               5                   10              


<210>  43
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  43

Arg Glu Asp Tyr Asp Tyr His Trp Tyr Phe Asp Val 
1               5                   10          


<210>  44
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  44
gacatccaga tgacccagag cccaagcagc ctgagcgcca gcgtgggtga cagagtgacc       60

atcacctgta gtaccagctc gagtgtaagt tacatgcact ggtaccagca gaagccaggt      120

aaggctccaa ggctgctgat ctacagcaca tccaacctgg cttctggtgt gccaagcaga      180

ttcagcggta gcggtagcgg taccgacttc accttcacca tcagcagcct ccagccagag      240

gacatcgcca cctactactg ccatcagtgg agtagttatc ccacgttcgg ccaagggacc      300

aaggtggaaa tcaaaggatc cacttccggt tcaggaaagc ccgggagtgg tgaaggtagc      360

actaaaggcc aggtccagct gcaggagagc ggtccaggtc ttgtgagacc tagccagacc      420

ctgagcctga cctgcaccgt gtctggcttc accatcagca gtggttatag ctggcactgg      480

gtgagacagc cacctggacg aggtcttgag tggattggat acatacagta cagtggtatc      540

actaactaca acccctctct caaaagtaga gtgacaatgc tggtagacac cagcaagaac      600

cagttcagcc tgagactcag cagcgtgaca gccgccgaca ccgcggtcta ttattgtgca      660

agagaagact atgattacca ctggtacttc gatgtctggg gtcaaggcag cacggtcacc      720

gtctcctcag gtgcggccgc cgag                                             744


