                         SEQUENCE LISTING

<110>  Charit - Universittsmedizin Berlin
 
<120>  CHIMERIC ANTIGEN RECEPTOR (CAR)-EXPRESSING CELLS RECOGNIZING CEA

<130>  XII 2207/20WO

<150>  EP20192398.4
<151>  2020-08-24

<150>  EP20205693.3
<151>  2020-11-04

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  1
atgggatgga gctgtatcat cctcttcctg gtagcaacag ctacaggcgt gcacagt          57


<210>  2
<211>  744
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR variable

<400>  2
gacatccaga tgacccagag cccaagcagc ctgagcgcca gcgtgggtga cagagtgacc       60

atcacctgta gtaccagctc gagtgtaagt tacatgcact ggtaccagca gaagccaggt      120

aaggctccaa ggctgctgat ctacagcaca tccaacctgg cttctggtgt gccaagcaga      180

ttcagcggta gcggtagcgg taccgacttc accttcacca tcagcagcct ccagccagag      240

gacatcgcca cctactactg ccatcagtgg agtagttatc ccacgttcgg ccaagggacc      300

aaggtggaaa tcaaaggatc cacttccggt tcaggaaagc ccgggagtgg tgaaggtagc      360

actaaaggcc aggtccagct gcaggagagc ggtccaggtc ttgtgagacc tagccagacc      420

ctgagcctga cctgcaccgt gtctggcttc accatcagca gtggttatag ctggcactgg      480

gtgagacagc cacctggacg aggtcttgag tggattggat acatacagta cagtggtatc      540

actaactaca acccctctct caaaagtaga gtgacaatgc tggtagacac cagcaagaac      600

cagttcagcc tgagactcag cagcgtgaca gccgccgaca ccgcggtcta ttattgtgca      660

agagaagact atgattacca ctggtacttc gatgtctggg gtcaaggcag cacggtcacc      720

gtctcctcag gtgcggccgc cgag                                             744


<210>  3
<211>  693
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  3
cccaaatctt gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg       60

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc      120

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac      180

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac      240

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc      300

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc      360

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag      420

gaaatgacca aaaaccaggt cagcctgacc tgcctggtca aaggccttct atccagcgac      480

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc      540

gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg      600

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac      660

acgcagaaga gcctctccct gtctccgggt aag                                   693


<210>  4
<211>  222
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  4
gatcccaaac tctgctactt ttgggtgctg gtggtggttg gtggagtcct ggcttgctat       60

agcttgctag taacagtggc ctttattatt ttctgggtga ggagtaagag gagcaggctc      120

ctgcacagtg actacatgaa catgactccc cgccgccccg ggcccacccg caagcattac      180

cagccctatg ccccaccacg cgacttcgca gcctatcgct cc                         222


<210>  5
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  5
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgcag agaaggaaga accctcagga aggcctgtac      180

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag      240

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac      300

acctacgacg cccttcatat gcaggctctg ccacctagc                             339


<210>  6
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  6
ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct       60

ggaccg                                                                  66


<210>  7
<211>  597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  7
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtt gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg gttggtgtcg tgggcggcct gctgggcagc      540

ctggtgctgc tagtctgggt cctggccgtc atctgctccc gggccgcacg agggtga         597


<210>  8
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter

<400>  8
taatcccagt gtggtggtac ggaattctct agactgccgg atccaagctg gaggaaaaac       60

tgtttcatac agaaggcgtg gaggaaaaac tgtttcatac agaaggcgtg gaggaaaaac      120

tgtttcatac agaaggcgtg gaggaaaaac tgtttcatac agaaggcgtc gcgaattcgc      180

ggagactcta gagggtatat aatggaagct cgatttccag cttggcattc cggtactgtt      240

ggtaaacacc aagcttcacc                                                  260


<210>  9
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  9
atggtgagca agggcgagga gctgttcacc ggggtggtga tggcaccaag aagggctcgc       60

ggatgccgga ccctcggtct cccggcgctg cttctgctgc tgctgctccg gccgccggcg      120

acgcggggc                                                              129


<210>  10
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  10
atcacgtgtc ctccacccat gtccgtggaa cacgcagaca tctgggtcaa gagctacagc       60

ttgtactcca gggagcggta catttgtaac tctggtttca agaggaaagc cggcacgtcc      120

agcctgacgg agtgcgtgtt gaacaaggcc acgaatgtcg cccactggac aacccccagt      180

ctcaaatgca ttagagaccc tgccctggtt caccaaaggc cagcgccacc c               231


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  11
agcggcggag gatgtggagg cggaggctgt ggcggaagcg gaggcggagg cagt             54


<210>  12
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  12
atccagaact gggtgaatgt cataagtgat ctgaagaaaa ttgaagatct tattcagtct       60

atgcatattg atgctactct atatactgaa agtgatgttc accccagttg caaagtgaca      120

gcaatgaagt gctttctctt ggagctgcaa gttatttcac ttgagtccgg agatgcaagt      180

attcatgata cagtggaaaa tctgatcatc cttgcaaacg acagtttgtc ttctaatggg      240

aatgtaacag aatctggatg caaagaatgt gaggaactgg aggaaaaaaa tattaaagaa      300

tttttgcaga gttttgtaca tattgtccaa atgttcatca acacttcttg ataa            354


<210>  13
<211>  3746
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  13
atgggatgga gctgtatcat cctcttcctg gtagcaacag ctacaggcgt gcacagtgac       60

atccagatga cccagagccc aagcagcctg agcgccagcg tgggtgacag agtgaccatc      120

acctgtagta ccagctcgag tgtaagttac atgcactggt accagcagaa gccaggtaag      180

gctccaaggc tgctgatcta cagcacatcc aacctggctt ctggtgtgcc aagcagattc      240

agcggtagcg gtagcggtac cgacttcacc ttcaccatca gcagcctcca gccagaggac      300

atcgccacct actactgcca tcagtggagt agttatccca cgttcggcca agggaccaag      360

gtggaaatca aaggatccac ttccggttca ggaaagcccg ggagtggtga aggtagcact      420

aaaggccagg tccagctgca ggagagcggt ccaggtcttg tgagacctag ccagaccctg      480

agcctgacct gcaccgtgtc tggcttcacc atcagcagtg gttatagctg gcactgggtg      540

agacagccac ctggacgagg tcttgagtgg attggataca tacagtacag tggtatcact      600

aactacaacc cctctctcaa aagtagagtg acaatgctgg tagacaccag caagaaccag      660

ttcagcctga gactcagcag cgtgacagcc gccgacaccg cggtctatta ttgtgcaaga      720

gaagactatg attaccactg gtacttcgat gtctggggtc aaggcagcac ggtcaccgtc      780

tcctcaggtg cggccgccga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc      840

ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac      900

accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa      960

gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca     1020

aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg     1080

caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca     1140

gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac     1200

accctgcccc catcccggga ggaaatgacc aaaaaccagg tcagcctgac ctgcctggtc     1260

aaaggccttc tatccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac     1320

aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag     1380

ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat     1440

gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg taaggatccc     1500

aaactctgct acttttgggt gctggtggtg gttggtggag tcctggcttg ctatagcttg     1560

ctagtaacag tggcctttat tattttctgg gtgaggagta agaggagcag gctcctgcac     1620

agtgactaca tgaacatgac tccccgccgc cccgggccca cccgcaagca ttaccagccc     1680

tatgccccac cacgcgactt cgcagcctat cgctccagag tgaagttcag caggagcgca     1740

gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa tctaggacga     1800

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag     1860

ccgcagagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg     1920

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat     1980

ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct tcatatgcag     2040

gctctgccac ctagcggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat     2100

gtcgaagaga atcctggacc gatgcagatc ccacaggcgc cctggccagt cgtctgggcg     2160

gtgctacaac tgggctggcg gccaggatgg ttcttagact ccccagacag gccctggaac     2220

ccccccacct tctccccagc cctgctcgtg gtgaccgaag gggacaacgc caccttcacc     2280

tgcagcttct ccaacacatc ggagagcttc gtgctaaact ggtaccgcat gagccccagc     2340

aaccagacgg acaagctggc cgccttcccc gaggaccgca gccagcccgg ccaggactgc     2400

cgcttccgtg tcacacaact gcccaacggg cgtgacttcc acatgagcgt tgtcagggcc     2460

cggcgcaatg acagcggcac ctacctctgt ggggccatct ccctggcccc caaggcgcag     2520

atcaaagaga gcctgcgggc agagctcagg gtgacagaga gaagggcaga agtgcccaca     2580

gcccacccca gcccctcacc caggccagcc ggccagttcc aaaccctggt ggttggtgtc     2640

gtgggcggcc tgctgggcag cctggtgctg ctagtctggg tcctggccgt catctgctcc     2700

cgggccgcac gagggtgata atcccagtgt ggtggtacgg aattctctag actgccggat     2760

ccaagctgga ggaaaaactg tttcatacag aaggcgtgga ggaaaaactg tttcatacag     2820

aaggcgtgga ggaaaaactg tttcatacag aaggcgtgga ggaaaaactg tttcatacag     2880

aaggcgtcgc gaattcgcgg agactctaga gggtatataa tggaagctcg atttccagct     2940

tggcattccg gtactgttgg taaacaccaa gcttcaccat ggtgagcaag ggcgaggagc     3000

tgttcaccgg ggtggtgatg gcaccaagaa gggctcgcgg atgccggacc ctcggtctcc     3060

cggcgctgct tctgctgctg ctgctccggc cgccggcgac gcggggcatc acgtgtcctc     3120

cacccatgtc cgtggaacac gcagacatct gggtcaagag ctacagcttg tactccaggg     3180

agcggtacat ttgtaactct ggtttcaaga ggaaagccgg cacgtccagc ctgacggagt     3240

gcgtgttgaa caaggccacg aatgtcgccc actggacaac ccccagtctc aaatgcatta     3300

gagaccctgc cctggttcac caaaggccag cgccacccag cggcggagga tgtggaggcg     3360

gaggctgtgg cggaagcgga ggcggaggca gtatccagaa ctgggtgaat gtcataagtg     3420

atctgaagaa aattgaagat cttattcagt ctatgcatat tgatgctact ctatatactg     3480

aaagtgatgt tcaccccagt tgcaaagtga cagcaatgaa gtgctttctc ttggagctgc     3540

aagttatttc acttgagtcc ggagatgcaa gtattcatga tacagtggaa aatctgatca     3600

tccttgcaaa cgacagtttg tcttctaatg ggaatgtaac agaatctgga tgcaaagaat     3660

gtgaggaact ggaggaaaaa aatattaaag aatttttgca gagttttgta catattgtcc     3720

aaatgttcat caacacttct tgataa                                          3746


<210>  14
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  14

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Thr Ala Thr 
1               5                   10                  15      


Gly Val His Ser 
            20  


<210>  15
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Thr Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu 
65                  70                  75                  80  


Asp Ile Ala Thr Tyr Tyr Cys His Gln Trp Ser Ser Tyr Pro Thr Phe 
                85                  90                  95      


Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Ser Thr Ser Gly Ser Gly 
            100                 105                 110         


Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val Gln Leu Gln 
        115                 120                 125             


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  16

Ser Ser Val Ser Tyr Met His 
1               5           


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  17

Leu Leu Ile Tyr Ser Thr Ser Asn Leu Ala Ser 
1               5                   10      


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  18

His Gln Trp Ser Ser Tyr Pro 
1               5           


<210>  19
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  19

Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu Ser Leu Thr 
1               5                   10                  15      


Cys Thr Val Ser Gly Phe Thr Ile Ser Ser Gly Tyr Ser Trp His Trp 
            20                  25                  30          


Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile Gly Tyr Ile Gln 
        35                  40                  45              


Tyr Ser Gly Ile Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr 
    50                  55                  60                  


Met Leu Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg Leu Ser Ser 
65                  70                  75                  80  


Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu Asp Tyr 
                85                  90                  95      


Asp Tyr His Trp Tyr Phe Asp Val Trp Gly Gln Gly Ser Thr Val Thr 
            100                 105                 110         


Val Ser Ser Gly Ala Ala Ala Glu 
        115                 120 


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  20

Phe Thr Ile Ser Ser Gly Tyr Ser Trp His 
1               5                   10  


<210>  21
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  21

Trp Ile Gly Tyr Ile Gln Tyr Ser Gly Ile Thr Asn Tyr 
1               5                   10              


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  22

Arg Glu Asp Tyr Asp Tyr His Trp Tyr Phe Asp Val 
1               5                   10          


<210>  23
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  23

Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Leu Leu Ser Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  24
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  24

Asp Pro Lys Leu Cys Tyr Phe Trp Val Leu Val Val Val Gly Gly Val 
1               5                   10                  15      


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            20                  25                  30          


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
        35                  40                  45              


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    50                  55                  60                  


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
65                  70                  


<210>  25
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  25

Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
1               5                   10                  15      


Val Thr Val Ala Phe Ile Ile 
            20              


<210>  26
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  26

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Ser 
    


<210>  27
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  27

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  28
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  28

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly 
        195             


<210>  29
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  29

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Met Ala Pro 
1               5                   10                  15      


Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala Leu Leu Leu 
            20                  25                  30          


Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg 
        35                  40          


<210>  30
<211>  78
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  30

Gly Ile Thr Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp 
1               5                   10                  15      


Val Lys Ser Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser 
            20                  25                  30          


Gly Phe Lys Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu 
        35                  40                  45              


Asn Lys Ala Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys 
    50                  55                  60                  


Ile Arg Asp Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro 
65                  70                  75              


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  31

Ser Gly Gly Gly Cys Gly Gly Gly Gly Cys Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser 
        


<210>  32
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  32

Ile Gln Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp 
1               5                   10                  15      


Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp 
            20                  25                  30          


Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu 
        35                  40                  45              


Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr 
    50                  55                  60                  


Val Glu Asn Leu Ile Ile Leu Ala Asn Asp Ser Leu Ser Ser Asn Gly 
65                  70                  75                  80  


Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys 
                85                  90                  95      


Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe 
            100                 105                 110         


Ile Asn Thr Ser 
        115     


<210>  33
<211>  685
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  33

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            20                  25                  30          


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Thr Ser Ser Ser Val 
        35                  40                  45              


Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Arg Leu 
    50                  55                  60                  


Leu Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu 
                85                  90                  95      


Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys His Gln Trp Ser Ser Tyr 
            100                 105                 110         


Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Ser Thr Ser 
        115                 120                 125             


Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val 
    130                 135                 140                 


Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg Pro Ser Gln Thr Leu 
145                 150                 155                 160 


Ser Leu Thr Cys Thr Val Ser Gly Phe Thr Ile Ser Ser Gly Tyr Ser 
                165                 170                 175     


Trp His Trp Val Arg Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile Gly 
            180                 185                 190         


Tyr Ile Gln Tyr Ser Gly Ile Thr Asn Tyr Asn Pro Ser Leu Lys Ser 
        195                 200                 205             


Arg Val Thr Met Leu Val Asp Thr Ser Lys Asn Gln Phe Ser Leu Arg 
    210                 215                 220                 


Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Glu Asp Tyr Asp Tyr His Trp Tyr Phe Asp Val Trp Gly Gln Gly Ser 
                245                 250                 255     


Thr Val Thr Val Ser Ser Gly Ala Ala Ala Glu Pro Lys Ser Cys Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
        275                 280                 285             


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
    290                 295                 300                 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
305                 310                 315                 320 


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
                325                 330                 335     


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
            340                 345                 350         


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
        355                 360                 365             


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
    370                 375                 380                 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
385                 390                 395                 400 


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
                405                 410                 415     


Thr Cys Leu Val Lys Gly Leu Leu Ser Ser Asp Ile Ala Val Glu Trp 
            420                 425                 430         


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
        435                 440                 445             


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
    450                 455                 460                 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
465                 470                 475                 480 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
                485                 490                 495     


Gly Lys Asp Pro Lys Leu Cys Tyr Phe Trp Val Leu Val Val Val Gly 
            500                 505                 510         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        515                 520                 525             


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
    530                 535                 540                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
545                 550                 555                 560 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
                565                 570                 575     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
            580                 585                 590         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        595                 600                 605             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg 
    610                 615                 620                 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
625                 630                 635                 640 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
                645                 650                 655     


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            660                 665                 670         


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Ser 
        675                 680                 685 


<210>  34
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  34

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly 
        195             


<210>  35
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct component

<400>  35

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Met Ala Pro 
1               5                   10                  15      


Arg Arg Ala Arg Gly Cys Arg Thr Leu Gly Leu Pro Ala Leu Leu Leu 
            20                  25                  30          


Leu Leu Leu Leu Arg Pro Pro Ala Thr Arg Gly Ile Thr Cys Pro Pro 
        35                  40                  45              


Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser Tyr Ser Leu 
    50                  55                  60                  


Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys Arg Lys Ala 
65                  70                  75                  80  


Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala Thr Asn Val 
                85                  90                  95      


Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp Pro Ala Leu 
            100                 105                 110         


Val His Gln Arg Pro Ala Pro Pro Ser Gly Gly Gly Cys Gly Gly Gly 
        115                 120                 125             


Gly Cys Gly Gly Ser Gly Gly Gly Gly Ser Ile Gln Asn Trp Val Asn 
    130                 135                 140                 


Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His 
145                 150                 155                 160 


Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys 
                165                 170                 175     


Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu 
            180                 185                 190         


Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile 
        195                 200                 205             


Leu Ala Asn Asp Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly 
    210                 215                 220                 


Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu 
225                 230                 235                 240 


Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
                245                 250                 


