                         SEQUENCE LISTING

<110>  Ligandal, Inc.
       Watson, Andre
 
<120>  COMPOSITIONS AND METHODS FOR NANOPARTICLE SEED SUBSTRATES

<130>  134554.8001.WO00

<150>  US 63/063,931
<151>  2020-08-21

<160>  88    

<170>  PatentIn version 3.5

<210>  1
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  Histone H2AX [119-143]

<220>
<221>  MISC_FEATURE
<222>  (27)..(30)
<223>  Cleavable domain

<220>
<221>  MISC_FEATURE
<222>  (31)..(40)
<223>  Linker domain

<220>
<221>  MISC_FEATURE
<222>  (41)..(44)
<223>  Cleavable domain

<220>
<221>  MISC_FEATURE
<222>  (45)..(65)
<223>  E-selectin ligand domain

<400>  1

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Phe Lys Phe Leu Met Ile Ala Ser 
        35                  40                  45              


Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly 
    50                  55                  60                  


Ala 
65  


<210>  2
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand


<220>
<221>  MISC_FEATURE
<222>  (1)..(26)
<223>  Histone H2AX [119-143]

<220>
<221>  MISC_FEATURE
<222>  (27)..(30)
<223>  Cleavable domain

<220>
<221>  MISC_FEATURE
<222>  (31)..(40)
<223>  Linker domain

<220>
<221>  MISC_FEATURE
<222>  (41)..(61)
<223>  E-selectin ligand domain

<400>  2

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser Gln Phe Leu Ser 
        35                  40                  45              


Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
    50                  55                  60      


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable domain

<400>  3

Phe Lys Phe Leu 
1               


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker domain

<400>  4

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E-selectin ligand domain

<400>  5

Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile 
1               5                   10                  15      


Lys Glu Ser Gly Ala 
            20      


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker domain

<400>  6

Gly Ala Pro Gly Ala Pro Gly Ala Pro 
1               5                   


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2 (31-45)

<400>  7

Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr 
1               5                   10                  15  


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2 (61-73)

<400>  8

Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala 
1               5                   10              


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Random coiled sequence

<400>  9

Lys Lys Lys Arg Lys 
1               5   


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Random coiled sequence

<400>  10

Lys Lys Ala His His His His His His 
1               5                   


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Random coiled sequence

<400>  11

Arg Arg Arg Arg Arg Arg Arg 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Random coiled sequence

<400>  12

Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   


<210>  13
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Histone H2AX [119-143]

<400>  13

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr 
            20                  25      


<210>  14
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Representative Gly-X-Y domain

<400>  14

Gly Ala Pro Gly Pro Ser Gly Ala Arg Gly Glu Arg Gly Phe Pro Gly 
1               5                   10                  15      


Glu Arg Gly Val Gln Gly 
            20          


<210>  15
<211>  589
<212>  PRT
<213>  Homo sapiens

<400>  15

Trp Ser Tyr Asn Thr Ser Thr Glu Ala Met Thr Tyr Asp Glu Ala Ser 
1               5                   10                  15      


Ala Tyr Cys Gln Gln Arg Tyr Thr His Leu Val Ala Ile Gln Asn Lys 
            20                  25                  30          


Glu Glu Ile Glu Tyr Leu Asn Ser Ile Leu Ser Tyr Ser Pro Ser Tyr 
        35                  40                  45              


Tyr Trp Ile Gly Ile Arg Lys Val Asn Asn Val Trp Val Trp Val Gly 
    50                  55                  60                  


Thr Gln Lys Pro Leu Thr Glu Glu Ala Lys Asn Trp Ala Pro Gly Glu 
65                  70                  75                  80  


Pro Asn Asn Arg Gln Lys Asp Glu Asp Cys Val Glu Ile Tyr Ile Lys 
                85                  90                  95      


Arg Glu Lys Asp Val Gly Met Trp Asn Asp Glu Arg Cys Ser Lys Lys 
            100                 105                 110         


Lys Leu Ala Leu Cys Tyr Thr Ala Ala Cys Thr Asn Thr Ser Cys Ser 
        115                 120                 125             


Gly His Gly Glu Cys Val Glu Thr Ile Asn Asn Tyr Thr Cys Lys Cys 
    130                 135                 140                 


Asp Pro Gly Phe Ser Gly Leu Lys Cys Glu Gln Ile Val Asn Cys Thr 
145                 150                 155                 160 


Ala Leu Glu Ser Pro Glu His Gly Ser Leu Val Cys Ser His Pro Leu 
                165                 170                 175     


Gly Asn Phe Ser Tyr Asn Ser Ser Cys Ser Ile Ser Cys Asp Arg Gly 
            180                 185                 190         


Tyr Leu Pro Ser Ser Met Glu Thr Met Gln Cys Met Ser Ser Gly Glu 
        195                 200                 205             


Trp Ser Ala Pro Ile Pro Ala Cys Asn Val Val Glu Cys Asp Ala Val 
    210                 215                 220                 


Thr Asn Pro Ala Asn Gly Phe Val Glu Cys Phe Gln Asn Pro Gly Ser 
225                 230                 235                 240 


Phe Pro Trp Asn Thr Thr Cys Thr Phe Asp Cys Glu Glu Gly Phe Glu 
                245                 250                 255     


Leu Met Gly Ala Gln Ser Leu Gln Cys Thr Ser Ser Gly Asn Trp Asp 
            260                 265                 270         


Asn Glu Lys Pro Thr Cys Lys Ala Val Thr Cys Arg Ala Val Arg Gln 
        275                 280                 285             


Pro Gln Asn Gly Ser Val Arg Cys Ser His Ser Pro Ala Gly Glu Phe 
    290                 295                 300                 


Thr Phe Lys Ser Ser Cys Asn Phe Thr Cys Glu Glu Gly Phe Met Leu 
305                 310                 315                 320 


Gln Gly Pro Ala Gln Val Glu Cys Thr Thr Gln Gly Gln Trp Thr Gln 
                325                 330                 335     


Gln Ile Pro Val Cys Glu Ala Phe Gln Cys Thr Ala Leu Ser Asn Pro 
            340                 345                 350         


Glu Arg Gly Tyr Met Asn Cys Leu Pro Ser Ala Ser Gly Ser Phe Arg 
        355                 360                 365             


Tyr Gly Ser Ser Cys Glu Phe Ser Cys Glu Gln Gly Phe Val Leu Lys 
    370                 375                 380                 


Gly Ser Lys Arg Leu Gln Cys Gly Pro Thr Gly Glu Trp Asp Asn Glu 
385                 390                 395                 400 


Lys Pro Thr Cys Glu Ala Val Arg Cys Asp Ala Val His Gln Pro Pro 
                405                 410                 415     


Lys Gly Leu Val Arg Cys Ala His Ser Pro Ile Gly Glu Phe Thr Tyr 
            420                 425                 430         


Lys Ser Ser Cys Ala Phe Ser Cys Glu Glu Gly Phe Glu Leu His Gly 
        435                 440                 445             


Ser Thr Gln Leu Glu Cys Thr Ser Gln Gly Gln Trp Thr Glu Glu Val 
    450                 455                 460                 


Pro Ser Cys Gln Val Val Lys Cys Ser Ser Leu Ala Val Pro Gly Lys 
465                 470                 475                 480 


Ile Asn Met Ser Cys Ser Gly Glu Pro Val Phe Gly Thr Val Cys Lys 
                485                 490                 495     


Phe Ala Cys Pro Glu Gly Trp Thr Leu Asn Gly Ser Ala Ala Arg Thr 
            500                 505                 510         


Cys Gly Ala Thr Gly His Trp Ser Gly Leu Leu Pro Thr Cys Glu Ala 
        515                 520                 525             


Pro Thr Glu Ser Asn Ile Pro Leu Val Ala Gly Leu Ser Ala Ala Gly 
    530                 535                 540                 


Leu Ser Leu Leu Thr Leu Ala Pro Phe Leu Leu Trp Leu Arg Lys Cys 
545                 550                 555                 560 


Leu Arg Lys Ala Lys Lys Phe Val Pro Ala Ser Ser Cys Gln Ser Leu 
                565                 570                 575     


Glu Ser Asp Gly Ser Tyr Gln Lys Pro Ser Tyr Ile Leu 
            580                 585                 


<210>  16
<211>  294
<212>  PRT
<213>  Homo sapiens

<400>  16

Trp Thr Tyr His Tyr Ser Glu Lys Pro Met Asn Trp Gln Arg Ala Arg 
1               5                   10                  15      


Arg Phe Cys Arg Asp Asn Tyr Thr Asp Leu Val Ala Ile Gln Asn Lys 
            20                  25                  30          


Ala Glu Ile Glu Tyr Leu Glu Lys Thr Leu Pro Phe Ser Arg Ser Tyr 
        35                  40                  45              


Tyr Trp Ile Gly Ile Arg Lys Ile Gly Gly Ile Trp Thr Trp Val Gly 
    50                  55                  60                  


Thr Asn Lys Ser Leu Thr Glu Glu Ala Glu Asn Trp Gly Asp Gly Glu 
65                  70                  75                  80  


Pro Asn Asn Lys Lys Asn Lys Glu Asp Cys Val Glu Ile Tyr Ile Lys 
                85                  90                  95      


Arg Asn Lys Asp Ala Gly Lys Trp Asn Asp Asp Ala Cys His Lys Leu 
            100                 105                 110         


Lys Ala Ala Leu Cys Tyr Thr Ala Ser Cys Gln Pro Trp Ser Cys Ser 
        115                 120                 125             


Gly His Gly Glu Cys Val Glu Ile Ile Asn Asn Tyr Thr Cys Asn Cys 
    130                 135                 140                 


Asp Val Gly Tyr Tyr Gly Pro Gln Cys Gln Phe Val Ile Gln Cys Glu 
145                 150                 155                 160 


Pro Leu Glu Ala Pro Glu Leu Gly Thr Met Asp Cys Thr His Pro Leu 
                165                 170                 175     


Gly Asn Phe Ser Phe Ser Ser Gln Cys Ala Phe Ser Cys Ser Glu Gly 
            180                 185                 190         


Thr Asn Leu Thr Gly Ile Glu Glu Thr Thr Cys Gly Pro Phe Gly Asn 
        195                 200                 205             


Trp Ser Ser Pro Glu Pro Thr Cys Gln Val Ile Gln Cys Glu Pro Leu 
    210                 215                 220                 


Ser Ala Pro Asp Leu Gly Ile Met Asn Cys Ser His Pro Leu Ala Ser 
225                 230                 235                 240 


Phe Ser Phe Thr Ser Ala Cys Thr Phe Ile Cys Ser Glu Gly Thr Glu 
                245                 250                 255     


Leu Ile Gly Lys Lys Lys Thr Ile Cys Glu Ser Ser Gly Ile Trp Ser 
            260                 265                 270         


Asn Pro Ser Pro Ile Cys Gln Lys Leu Asp Lys Ser Phe Ser Met Ile 
        275                 280                 285             


Lys Glu Gly Asp Tyr Asn 
    290                 


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Predicted binding domain

<400>  17

Leu Pro Tyr Tyr Ser Ser Tyr 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Predicted binding domain

<400>  18

Leu Ser Tyr Ser Pro Ser Tyr 
1               5           


<210>  19
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Predicted binding domain

<400>  19

Cys Val Glu Ile Tyr Ile Lys Ser Pro Ser Ala Pro Gly Lys Trp Asn 
1               5                   10                  15      


Asp Glu His Cys 
            20  


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Predicted binding domain

<400>  20

Cys Val Glu Ile Tyr Ile Lys Arg Glu Lys Asp Val Gly Met Trp Asp 
1               5                   10                  15      


Glu Arg Cys 
            


<210>  21
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCF (39-56)

<400>  21

Leu Pro Ser His Cys Trp Ile Ser Glu Met Val Val Gln Leu Ser Asp 
1               5                   10                  15      


Ser Leu 
        


<210>  22
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfRH5 (346-357)

<400>  22

Tyr Asn Asn Asn Phe Cys Asn Thr Asn Gly Ile Arg Tyr His 
1               5                   10                  


<210>  23
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfRH5 (200-208)

<400>  23

Tyr Gly Lys Tyr Ile Ala Val Asp Ala 
1               5                   


<210>  24
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfRH5 (444-453)

<400>  24

Leu Asn Ile Trp Arg Thr Phe Gln Lys Asp 
1               5                   10  


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRPa binding domain


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Optional I

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Optional Q

<400>  25

Leu Ile Pro Val Gly Pro Xaa Xaa 
1               5               


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRPa binding domain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Optional F

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Optional R

<400>  26

Xaa Xaa Lys Gly Ser Pro 
1               5       


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SIRPa binding domain

<400>  27

Thr Lys Arg Glu 
1               


<210>  28
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  28

Leu Ile Pro Val Gly Pro Ile Gly Gly Phe Arg Lys Gly Ser Pro 
1               5                   10                  15  


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  29

Leu Ile Pro Val Gly Pro Ile Gly Gly Asn Gln Lys 
1               5                   10          


<210>  30
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  30

Leu Ile Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Ala 
1               5                   10                  15      


Arg Glu Leu Ile Tyr Asn Gln Lys 
            20                  


<210>  31
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  31

Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser Glu Ser 
1               5                   10                  15      


Thr Lys Arg Glu 
            20  


<210>  32
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  32

Leu Ile Pro Val Gly Pro Ile Gly Gly Asn Gln Lys Gly Gly Gly Gly 
1               5                   10                  15      


Ser Thr Lys Arg Glu 
            20      


<210>  33
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-ligand

<400>  33

Leu Ile Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Ala 
1               5                   10                  15      


Arg Glu Leu Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr 
            20                  25                  30          


Thr Val Ser Glu Ser Thr Lys Arg Glu 
        35                  40      


<210>  34
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Predicted binding sequence

<400>  34

Leu Ile Pro Val Gly Pro Gly Gly Phe Arg Lys Gly Ser Pro 
1               5                   10                  


<210>  35
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp120 binding domain

<400>  35

Asn Ala Lys Thr 
1               


<210>  36
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp120 binding domain

<400>  36

Ser Gly Gly Asp 
1               


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp120 binding domain

<400>  37

Trp Gln Lys Val Gly Lys Ala Met 
1               5               


<210>  38
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp120 binding domain

<400>  38

Arg Ser Val Asn Phe Thr Asp Asn Ala Lys Thr 
1               5                   10      


<210>  39
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  H2AX catalytic domain

<400>  39

Lys Lys Ala Thr Gln Ala Ser Glu Pro Gln Glu Tyr 
1               5                   10          


<210>  40
<211>  339
<212>  PRT
<213>  Homo sapiens

<400>  40

Met Ala Met Gln Met Gln Leu Glu Ala Asn Ala Asp Thr Ser Val Glu 
1               5                   10                  15      


Glu Glu Ser Phe Gly Pro Gln Pro Ile Ser Arg Leu Glu Gln Cys Gly 
            20                  25                  30          


Ile Asn Ala Asn Asp Val Lys Lys Leu Glu Glu Ala Gly Phe His Thr 
        35                  40                  45              


Val Glu Ala Val Ala Tyr Ala Pro Lys Lys Glu Leu Ile Asn Ile Lys 
    50                  55                  60                  


Gly Ile Ser Glu Ala Lys Ala Asp Lys Ile Leu Ala Glu Ala Ala Lys 
65                  70                  75                  80  


Leu Val Pro Met Gly Phe Thr Thr Ala Thr Glu Phe His Gln Arg Arg 
                85                  90                  95      


Ser Glu Ile Ile Gln Ile Thr Thr Gly Ser Lys Glu Leu Asp Lys Leu 
            100                 105                 110         


Leu Gln Gly Gly Ile Glu Thr Gly Ser Ile Thr Glu Met Phe Gly Glu 
        115                 120                 125             


Phe Arg Thr Gly Lys Thr Gln Ile Cys His Thr Leu Ala Val Thr Cys 
    130                 135                 140                 


Gln Leu Pro Ile Asp Arg Gly Gly Gly Glu Gly Lys Ala Met Tyr Ile 
145                 150                 155                 160 


Asp Thr Glu Gly Thr Phe Arg Pro Glu Arg Leu Leu Ala Val Ala Glu 
                165                 170                 175     


Arg Tyr Gly Leu Ser Gly Ser Asp Val Leu Asp Asn Val Ala Tyr Ala 
            180                 185                 190         


Arg Ala Phe Asn Thr Asp His Gln Thr Gln Leu Leu Tyr Gln Ala Ser 
        195                 200                 205             


Ala Met Met Val Glu Ser Arg Tyr Ala Leu Leu Ile Val Asp Ser Ala 
    210                 215                 220                 


Thr Ala Leu Tyr Arg Thr Asp Tyr Ser Gly Arg Gly Glu Leu Ser Ala 
225                 230                 235                 240 


Arg Gln Met His Leu Ala Arg Phe Leu Arg Met Leu Leu Arg Leu Ala 
                245                 250                 255     


Asp Glu Phe Gly Val Ala Val Val Ile Thr Asn Gln Val Val Ala Gln 
            260                 265                 270         


Val Asp Gly Ala Ala Met Phe Ala Ala Asp Pro Lys Lys Pro Ile Gly 
        275                 280                 285             


Gly Asn Ile Ile Ala His Ala Ser Thr Thr Arg Leu Tyr Leu Arg Lys 
    290                 295                 300                 


Gly Arg Gly Glu Thr Arg Ile Cys Lys Ile Tyr Asp Ser Pro Cys Leu 
305                 310                 315                 320 


Pro Glu Ala Glu Ala Met Phe Ala Ile Asn Ala Asp Gly Val Gly Asp 
                325                 330                 335     


Ala Lys Asp 
            


<210>  41
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes

<400>  41

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  42
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpCas9 mutant

<400>  42

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Asp Val Pro Ser Asp Asp Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Glu His Ser Ile Asp Asp Asp Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Asp Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Asp Leu Phe Ile Glu Leu Val Glu Thr Tyr 
            180                 185                 190         


Asn Glu Leu Phe Glu Glu Asn Pro Ile Asn Ala Asp Asp Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Asp Asp Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Gly Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Asp Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Asp Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Glu Val Asn Thr Glu Glu Thr Asp Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Asp Pro Ile Leu Glu Asp Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Asp Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu Glu Ala Ile Leu Glu Glu Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Glu Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Asp 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Glu Asp Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr Glu Asp Leu Leu Lys Ile Ile Asp Asp Asp Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Gly Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Asp Val Met Lys Gln Leu Asp Arg Arg Glu Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Glu Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Glu 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Asp Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  43
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified IL2 (31-44)


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Any amino acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Any amino acid

<400>  43

Tyr Lys Asn Xaa Lys Leu Thr Arg Met Leu Thr Xaa Lys Phe 
1               5                   10                  


<210>  44
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified IL2 (61-73)


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Any amino acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Any amino acid

<400>  44

Glu Glu Leu Xaa Xaa Leu Glu Glu Val Leu Asn Xaa Ala 
1               5                   10              


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand

<400>  45

Tyr Thr Ser Arg Leu His Ser Gly Val 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand

<400>  46

Leu Gly Leu Arg Gly His Ser Gly Val 
1               5                   


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand

<400>  47

Leu Leu Gly Arg Gly His Ser Gly Val 
1               5                   


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ligand

<400>  48

Gly Leu Leu Arg Gly His Ser Gly Val 
1               5                   


<210>  49
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adenosine deaminase (9-155)

<400>  49

Arg Gln Pro Ile Pro Ser Glu Gly Leu Gln Leu His Leu Pro Gln Val 
1               5                   10                  15      


Leu Ala Asp Ala Val Ser Arg Leu Val Leu Gly Lys Phe Gly Asp Leu 
            20                  25                  30          


Thr Asp Asn Phe Ser Ser Pro His Ala Arg Arg Lys Val Leu Ala Gly 
        35                  40                  45              


Val Val Met Thr Thr Gly Thr Asp Val Lys Asp Ala Lys Val Ile Ser 
    50                  55                  60                  


Val Ser Thr Gly Thr Lys Cys Ile Asn Gly Glu Tyr Met Ser Asp Arg 
65                  70                  75                  80  


Gly Leu Ala Leu Asn Asp Cys His Ala Glu Ile Ile Ser Arg Arg Ser 
                85                  90                  95      


Leu Leu Arg Phe Leu Tyr Thr Gln Leu Glu Leu Tyr Leu Asn Asn Lys 
            100                 105                 110         


Asp Asp Gln Lys Arg Ser Ile Phe Gln Lys Ser Glu Arg Gly Gly Phe 
        115                 120                 125             


Arg Leu Lys Glu Asn Val Gln Phe His Leu Tyr Ile Ser Thr Ser Pro 
    130                 135                 140                 


Cys Gly Asp 
145         


<210>  50
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dCpf1-crRNA-DNA complex

<400>  50

Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu 
1               5                   10                  15      


Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe 
            20                  25                  30          


Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro 
        35                  40                  45              


Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu 
    50                  55                  60                  


Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg 
65                  70                  75                  80  


Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala 
                85                  90                  95      


Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn 
            100                 105                 110         


Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu 
        115                 120                 125             


Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr 
    130                 135                 140                 


Thr Glu His 
145         


<210>  51
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2819

<400>  51

Val Gly Ala Leu Ala Val Val Val Trp Leu Trp Leu Trp Leu Trp Ala 
1               5                   10                  15      


Gly Ser Gly Pro Lys Lys Lys Arg Lys Val Cys 
            20                  25          


<210>  52
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2845

<400>  52

Lys Lys Trp Ala Leu Leu Ala Leu Ala Leu His His Leu Ala His Leu 
1               5                   10                  15      


Ala Leu His Leu Ala Leu Ala Leu Lys Lys Ala His His His His His 
            20                  25                  30          


His 
    


<210>  53
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2906

<400>  53

Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Gly Leu Phe Gly Ala 
1               5                   10                  15      


Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Ile Asp Gly Trp 
            20                  25                  30          


Tyr Gly 
        


<210>  54
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2926

<400>  54

Arg Phe Thr Phe His Phe Arg Phe Glu Phe Thr Phe His Phe Glu Gly 
1               5                   10                  15      


Gly Gly Arg Arg Arg Arg Arg Arg Arg 
            20                  25  


<210>  55
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2946

<400>  55

Leu Ala Gln Leu Leu Ala Gln Leu Leu Ala Gln Leu Gly Gly Gly Gly 
1               5                   10                  15      


Arg Arg Arg Arg Arg Arg Arg Arg Arg 
            20                  25  


<210>  56
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CPP 2959

<400>  56

Cys Gly Gly Gly Ala Arg Lys Lys Ala Ala Lys Ala Ala Arg Lys Lys 
1               5                   10                  15      


Ala Ala Lys Ala Ala Arg Lys Lys Ala Ala Lys Ala Ala Arg Lys Lys 
            20                  25                  30          


Ala Ala Lys Ala 
        35      


<210>  57
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  57

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Phe Lys Phe 
        35                  40                  45              


Leu Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu 
    50                  55                  60                  


Ile Lys Glu Ser Gly Ala 
65                  70  


<210>  58
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  58

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Phe Lys Phe Leu Met Ile Ala Ser Gln Phe Leu Ser Ala Leu 
    50                  55                  60                  


Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
65                  70                  75  


<210>  59
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  59

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Phe Phe Leu Met Ile Ala Ser Gln Phe 
        35                  40                  45              


Leu Ser Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
    50                  55                  60              


<210>  60
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  60

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Phe Lys Phe 
        35                  40                  45              


Leu Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu 
    50                  55                  60                  


Ile Lys Glu Ser Gly Ala 
65                  70  


<210>  61
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  61

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro 
        35                  40                  45              


Phe Phe Lys Phe Leu Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr 
    50                  55                  60                  


Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
65                  70                  


<210>  62
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  62

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Pro Ser Gly Ala Arg Gly Glu Arg Gly Phe Pro Gly Glu Arg 
        35                  40                  45              


Gly Val Gln Gly Phe Lys Phe Leu Met Ile Ala Ser Gln Phe Leu Ser 
    50                  55                  60                  


Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
65                  70                  75          


<210>  63
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  63

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser Gln Phe Leu Ser 
        35                  40                  45              


Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
    50                  55                  60      


<210>  64
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  64

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala 
        35                  40                  45              


Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser 
    50                  55                  60                  


Gly Ala 
65      


<210>  65
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  65

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu 
    50                  55                  60                  


Leu Ile Lys Glu Ser Gly Ala 
65                  70      


<210>  66
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  66

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Met Ile Ala Ser Gln Phe Leu Ser Ala 
        35                  40                  45              


Leu Thr Leu Val Leu Leu Ile Lys Glu Ser Gly Ala 
    50                  55                  60  


<210>  67
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  67

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Met Ile Ala 
        35                  40                  45              


Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser 
    50                  55                  60                  


Gly Ala 
65      


<210>  68
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  68

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro Gly Ala Pro 
        35                  40                  45              


Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile 
    50                  55                  60                  


Lys Glu Ser Gly Ala 
65                  


<210>  69
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anchor-linker-ligand

<400>  69

Lys Lys Arg Thr Ser Ala Thr Val Gly Pro Lys Ala Pro Ser Gly Gly 
1               5                   10                  15      


Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Ala 
            20                  25                  30          


Pro Gly Pro Ser Gly Ala Arg Gly Glu Arg Gly Phe Pro Gly Glu Arg 
        35                  40                  45              


Gly Val Gln Gly Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu 
    50                  55                  60                  


Val Leu Leu Ile Lys Glu Ser Gly Ala 
65                  70              


<210>  70
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c6bk4a

<400>  70

Val Gly Pro Lys Ala Pro Ser Gly Gly Lys Lys Ala Thr Gln Ala Ser 
1               5                   10                  15      


Gln Glu Tyr Phe Lys Phe Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25                  30          


Ser 
    


<210>  71
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  d2a5yb3

<400>  71

Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser Gln Phe Leu Ser 
1               5                   10                  15      


<210>  72
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker domain

<400>  72

Gly Gly Gly Gly Ser Gly Gly Gly 
1               5               


<210>  73
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c1p68A

<400>  73

Phe Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser 
1               5                   10                  15      


Gln Phe Leu Ser Ala Leu 
            20          


<210>  74
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c1psmA

<400>  74

Lys Lys Ala Thr Gln Ala Ser Gln Glu 
1               5                   


<210>  75
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c2i7uA

<400>  75

Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Met Ile 
            20  


<210>  76
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker domain

<400>  76

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  77
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c21qhB

<400>  77

Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser Gln Phe Leu 
1               5                   10                  15      


Ser Ala 
        


<210>  78
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker domain

<400>  78

Gly Gly Gly Ser Gly Gly Gly 
1               5           


<210>  79
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c2m86A

<400>  79

Ser Gly Gly Lys Lys Ala Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe 
1               5                   10                  15      


Leu Gly Gly Gly Gly 
            20      


<210>  80
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c2n5dA

<400>  80

Gly Gly Ser Gly Gly Gly Gly Ser Met Ile Ala Ser Gln Phe Leu Ser 
1               5                   10                  15      


Ala Leu 
        


<210>  81
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c4dzoA

<400>  81

Phe Leu Ser Ala Leu Thr Leu Val Leu Leu Ile Lys Glu Ser 
1               5                   10                  


<210>  82
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c4zdtB

<400>  82

Gly Ser Met Ile Ala Ser Gln Phe Leu Ser Ala Leu Thr Leu Val Leu 
1               5                   10                  15      


<210>  83
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c5zcsE/c5zcsF

<400>  83

Thr Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly 
1               5                   10          


<210>  84
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c6musB

<400>  84

Gln Ala Ser Gln Glu Tyr Phe Lys Phe Leu Gly Gly Gly 
1               5                   10              


<210>  85
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  d1k47a2/d1kkha2/k1kvka2

<400>  85

Gly Ser Gly Gly Gly Gly Ser Met Ile Ala 
1               5                   10  


<210>  86
<211>  185
<212>  PRT
<213>  Human immunodeficiency virus

<400>  86

Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile 
1               5                   10                  15      


Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu 
            20                  25                  30          


Glu Glu Val Val Ile Arg Ser Val Asn Phe Thr Asp Asn Ala Lys Thr 
        35                  40                  45              


Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Asn Cys Thr Gly Ala 
    50                  55                  60                  


Gly His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu Lys Gln 
65                  70                  75                  80  


Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly Asn Asn Lys Thr Ile Ile 
                85                  90                  95      


Phe Lys Gln Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His Ser Phe 
            100                 105                 110         


Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn 
        115                 120                 125             


Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Lys Gly Ser Asn Asn Thr 
    130                 135                 140                 


Glu Gly Ser Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile 
145                 150                 155                 160 


Asn Met Trp Gln Lys Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Ser 
                165                 170                 175     


Gly Gln Ile Arg Cys Ser Ser Asn Ile 
            180                 185 


<210>  87
<211>  133
<212>  PRT
<213>  Homo sapiens

<400>  87

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  88
<211>  34
<212>  PRT
<213>  Homo sapiens

<400>  88

Asp Pro Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr 
1               5                   10                  15      


Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys 
            20                  25                  30          


Lys Arg 
        


