                         SEQUENCE LISTING

<110>  Vaxcyte, Inc.
 
<120>  CARRIER-PROTEIN POLYSACCHARIDE CONJUGATION METHODS

<130>  STRO-012/01WO 331540-2103

<150>  US 63/067,752
<151>  2020-08-19

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  535
<212>  PRT
<213>  Unknown

<220>
<223>  CRM197

<400>  1

Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      


Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          


Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              


Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  


Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  


Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      


Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         


Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             


Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 


Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 


Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     


Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         


Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             


Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 


Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 


Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     


His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         


Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             


Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 


Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     


Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         


Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             


Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 


Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 


Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     


Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         


Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             


His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 


Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 


Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     


Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         


Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 
        515                 520                 525             


Leu Phe Phe Glu Ile Lys Ser 
    530                 535 


<210>  2
<211>  536
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CRM-pAMF6 mutant


<220>
<221>  SITE
<222>  (34)..(34)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<220>
<221>  SITE
<222>  (213)..(213)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<220>
<221>  SITE
<222>  (245)..(245)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<220>
<221>  SITE
<222>  (265)..(265)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<220>
<221>  SITE
<222>  (386)..(386)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<220>
<221>  SITE
<222>  (527)..(527)
<223>  Xaa is 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid

<400>  2

Met Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Xaa Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Asn Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Xaa Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Xaa Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Xaa Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Xaa Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn 
385                 390                 395                 400 


Thr Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly 
                405                 410                 415     


His Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly 
            420                 425                 430         


Val Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys 
        435                 440                 445             


Thr His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala 
    450                 455                 460                 


Ile Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val 
465                 470                 475                 480 


Gly Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser 
                485                 490                 495     


Ser Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val 
            500                 505                 510         


Leu Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Xaa Leu 
        515                 520                 525             


Ser Leu Phe Phe Glu Ile Lys Ser 
    530                 535     


<210>  3
<211>  580
<212>  PRT
<213>  Unknown

<220>
<223>  WT IpaB

<400>  3

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Lys Ser Asp Glu Tyr Ala Ala Glu Val Arg Lys Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Lys 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Lys Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Lys Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  4
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 1 K289/K368/K395


<220>
<221>  SITE
<222>  (289)..(289)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (368)..(368)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<400>  4

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Xaa Ser Asp Glu Tyr Ala Ala Glu Val Arg Lys Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Xaa 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Lys Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  5
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 2 K299/K395/K436


<220>
<221>  SITE
<222>  (299)..(299)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (436)..(436)
<223>  Xaa is a non-natural amino acid

<400>  5

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Lys Ser Asp Glu Tyr Ala Ala Glu Val Arg Xaa Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Lys 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Xaa Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  6
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 3 K299/K368/K395


<220>
<221>  SITE
<222>  (299)..(299)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (368)..(368)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<400>  6

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Lys Ser Asp Glu Tyr Ala Ala Glu Val Arg Xaa Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Xaa 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Lys Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  7
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 4 K289/K368/K395/K436


<220>
<221>  SITE
<222>  (289)..(289)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (368)..(368)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (436)..(436)
<223>  Xaa is a non-natural amino acid

<400>  7

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Xaa Ser Asp Glu Tyr Ala Ala Glu Val Arg Lys Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Xaa 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Xaa Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  8
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 5 K299/K395/K436/K470


<220>
<221>  SITE
<222>  (299)..(299)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (436)..(436)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (470)..(470)
<223>  Xaa is a non-natural amino acid

<400>  8

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Lys Ser Asp Glu Tyr Ala Ala Glu Val Arg Xaa Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Lys 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Xaa Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Xaa Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  9
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IpaB Mutant 6 K299/K368/K395/K436


<220>
<221>  SITE
<222>  (299)..(299)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (368)..(368)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (395)..(395)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (436)..(436)
<223>  Xaa is a non-natural amino acid

<400>  9

Met His Asn Val Asn Thr Thr Thr Thr Gly Leu Ser Leu Ala Lys Ile 
1               5                   10                  15      


Leu Ala Ser Thr Glu Leu Gly Asp Asn Thr Ile Gln Ala Gly Asn Asp 
            20                  25                  30          


Ala Ala Asn Lys Leu Phe Ser Leu Thr Ile Ala Asp Leu Thr Ala Asn 
        35                  40                  45              


Lys Asn Ile Asn Thr Thr Asn Ala His Ser Thr Ser Asn Ile Leu Ile 
    50                  55                  60                  


Pro Glu Leu Lys Ala Pro Lys Ser Leu Asn Ala Ser Ser Gln Leu Thr 
65                  70                  75                  80  


Leu Leu Ile Gly Asn Leu Ile Gln Ile Leu Gly Glu Lys Ser Leu Thr 
                85                  90                  95      


Ala Leu Thr Asn Lys Ile Thr Ala Trp Lys Ser Gln Gln Gln Ala Arg 
            100                 105                 110         


Gln Gln Lys Asn Leu Glu Phe Ser Asp Lys Ile Asn Thr Leu Leu Ser 
        115                 120                 125             


Glu Thr Glu Gly Leu Thr Arg Asp Tyr Glu Lys Gln Ile Asn Lys Leu 
    130                 135                 140                 


Lys Asn Ala Asp Ser Lys Ile Lys Asp Leu Glu Asn Lys Ile Asn Gln 
145                 150                 155                 160 


Ile Gln Thr Arg Leu Ser Glu Leu Asp Pro Asp Ser Pro Glu Lys Lys 
                165                 170                 175     


Lys Leu Ser Arg Glu Glu Ile Gln Leu Thr Ile Lys Lys Asp Ala Ala 
            180                 185                 190         


Val Lys Asp Arg Thr Leu Ile Glu Gln Lys Thr Leu Ser Ile His Ser 
        195                 200                 205             


Lys Leu Thr Asp Lys Ser Met Gln Leu Glu Lys Glu Ile Asp Ser Phe 
    210                 215                 220                 


Ser Ala Phe Ser Asn Thr Ala Ser Ala Glu Gln Leu Ser Thr Gln Gln 
225                 230                 235                 240 


Lys Ser Leu Thr Gly Leu Ala Ser Val Thr Gln Leu Met Ala Thr Phe 
                245                 250                 255     


Ile Gln Leu Val Gly Lys Asn Asn Glu Glu Ser Leu Lys Asn Asp Leu 
            260                 265                 270         


Ala Leu Phe Gln Ser Leu Gln Glu Ser Arg Lys Thr Glu Met Glu Arg 
        275                 280                 285             


Lys Ser Asp Glu Tyr Ala Ala Glu Val Arg Xaa Ala Glu Glu Leu Asn 
    290                 295                 300                 


Arg Val Met Gly Cys Val Gly Lys Ile Leu Gly Ala Leu Leu Thr Ile 
305                 310                 315                 320 


Val Ser Val Val Ala Ala Ala Phe Ser Gly Gly Ala Ser Leu Ala Leu 
                325                 330                 335     


Ala Asp Val Gly Leu Ala Leu Met Val Thr Asp Ala Ile Val Gln Ala 
            340                 345                 350         


Ala Thr Gly Asn Ser Phe Met Glu Gln Ala Leu Asn Pro Ile Met Xaa 
        355                 360                 365             


Ala Val Ile Glu Pro Leu Ile Lys Leu Leu Ser Asp Ala Phe Thr Lys 
    370                 375                 380                 


Met Leu Glu Gly Leu Gly Val Asp Ser Lys Xaa Ala Lys Met Ile Gly 
385                 390                 395                 400 


Ser Ile Leu Gly Ala Ile Ala Gly Ala Leu Val Leu Val Ala Ala Val 
                405                 410                 415     


Val Leu Val Ala Thr Val Gly Lys Gln Ala Ala Ala Lys Leu Ala Glu 
            420                 425                 430         


Asn Ile Gly Xaa Ile Ile Gly Lys Thr Leu Thr Asp Leu Ile Pro Lys 
        435                 440                 445             


Phe Leu Lys Asn Phe Ser Ser Gln Leu Asp Asp Leu Ile Thr Asn Ala 
    450                 455                 460                 


Val Ala Arg Leu Asn Lys Phe Leu Gly Ala Ala Gly Asp Glu Val Ile 
465                 470                 475                 480 


Ser Lys Gln Ile Ile Ser Thr His Leu Asn Gln Ala Val Leu Leu Gly 
                485                 490                 495     


Glu Ser Val Asn Ser Ala Thr Gln Ala Gly Gly Ser Val Ala Ser Ala 
            500                 505                 510         


Val Phe Gln Asn Ser Ala Ser Thr Asn Leu Ala Asp Leu Thr Leu Ser 
        515                 520                 525             


Lys Tyr Gln Val Glu Gln Leu Ser Lys Tyr Ile Ser Glu Ala Ile Glu 
    530                 535                 540                 


Lys Phe Gly Gln Leu Gln Glu Val Ile Ala Asp Leu Leu Ala Ser Met 
545                 550                 555                 560 


Ser Asn Ser Gln Ala Asn Arg Thr Asp Val Ala Lys Ala Ile Leu Gln 
                565                 570                 575     


Gln Thr Thr Ala 
            580 


<210>  10
<211>  834
<212>  PRT
<213>  Streptococcus pyogenes

<400>  10

Met His His His His His His Gly Ser Gly Glu Asn Leu Tyr Phe Gln 
1               5                   10                  15      


Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
            20                  25                  30          


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
        35                  40                  45              


Lys Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
    50                  55                  60                  


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
65                  70                  75                  80  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
                85                  90                  95      


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
            100                 105                 110         


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
        115                 120                 125             


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
    130                 135                 140                 


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
145                 150                 155                 160 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
                165                 170                 175     


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
            180                 185                 190         


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
        195                 200                 205             


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
    210                 215                 220                 


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
225                 230                 235                 240 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
                245                 250                 255     


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Lys 
            260                 265                 270         


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
        275                 280                 285             


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
    290                 295                 300                 


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
305                 310                 315                 320 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
                325                 330                 335     


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
            340                 345                 350         


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
        355                 360                 365             


Tyr Lys Lys Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
    370                 375                 380                 


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
385                 390                 395                 400 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
                405                 410                 415     


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
            420                 425                 430         


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
        435                 440                 445             


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
    450                 455                 460                 


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
465                 470                 475                 480 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
                485                 490                 495     


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
            500                 505                 510         


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
        515                 520                 525             


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
    530                 535                 540                 


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
545                 550                 555                 560 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
                565                 570                 575     


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
            580                 585                 590         


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
        595                 600                 605             


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
    610                 615                 620                 


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
625                 630                 635                 640 


Lys Lys Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
                645                 650                 655     


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
            660                 665                 670         


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
        675                 680                 685             


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
    690                 695                 700                 


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
705                 710                 715                 720 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
                725                 730                 735     


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
            740                 745                 750         


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
        755                 760                 765             


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
    770                 775                 780                 


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
785                 790                 795                 800 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
                805                 810                 815     


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
            820                 825                 830         


Gln Arg 
        


<210>  11
<211>  251
<212>  PRT
<213>  Haemophilus influenzae

<400>  11

Gly Cys Ser Ser His Ser Ser Asn Met Ala Asn Thr Gln Met Lys Ser 
1               5                   10                  15      


Asp Lys Ile Ile Ile Ala His Arg Gly Ala Ser Gly Tyr Leu Pro Glu 
            20                  25                  30          


His Thr Leu Glu Ser Lys Ala Leu Ala Phe Ala Gln Gln Ala Asp Tyr 
        35                  40                  45              


Leu Glu Gln Asp Leu Ala Met Thr Lys Asp Gly Arg Leu Val Val Ile 
    50                  55                  60                  


His Asp His Phe Leu Asp Gly Leu Thr Asp Val Ala Lys Lys Phe Pro 
65                  70                  75                  80  


His Arg His Arg Lys Asp Gly Arg Tyr Tyr Val Ile Asp Phe Thr Leu 
                85                  90                  95      


Lys Glu Ile Gln Ser Leu Glu Met Thr Glu Asn Phe Glu Thr Lys Asp 
            100                 105                 110         


Gly Lys Gln Ala Gln Val Tyr Pro Asn Arg Phe Pro Leu Trp Lys Ser 
        115                 120                 125             


His Phe Arg Ile His Thr Phe Glu Asp Glu Ile Glu Phe Ile Gln Gly 
    130                 135                 140                 


Leu Glu Lys Ser Thr Gly Lys Lys Val Gly Ile Tyr Pro Glu Ile Lys 
145                 150                 155                 160 


Ala Pro Trp Phe His His Gln Asn Gly Lys Asp Ile Ala Ala Glu Thr 
                165                 170                 175     


Leu Lys Val Leu Lys Lys Tyr Gly Tyr Asp Lys Lys Thr Asp Met Val 
            180                 185                 190         


Tyr Leu Gln Thr Phe Asp Phe Asn Glu Leu Lys Arg Ile Lys Thr Glu 
        195                 200                 205             


Leu Leu Pro Gln Met Gly Met Asp Leu Lys Leu Val Gln Leu Ile Ala 
    210                 215                 220                 


Tyr Thr Asp Trp Lys Glu Thr Gln Glu Lys Asp Pro Lys Gly Tyr Trp 
225                 230                 235                 240 


Val Asn Tyr Asn Tyr Asp Trp Met Phe Lys Pro 
                245                 250     


<210>  12
<211>  817
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPYAD [33-849 FRAGMENT] WT W/O LEADER

<400>  12

Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser Asn 
1               5                   10                  15      


Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala Lys 
            20                  25                  30          


Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala Glu 
        35                  40                  45              


Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile Asn 
    50                  55                  60                  


His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser Ala 
65                  70                  75                  80  


Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu Leu 
                85                  90                  95      


Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr Glu 
            100                 105                 110         


Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu Ser 
        115                 120                 125             


Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp Leu 
    130                 135                 140                 


Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn Ala 
145                 150                 155                 160 


Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala Asn 
                165                 170                 175     


Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala Asp 
            180                 185                 190         


Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu Leu 
        195                 200                 205             


Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser Arg 
    210                 215                 220                 


Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn Asn 
225                 230                 235                 240 


Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Lys Leu 
                245                 250                 255     


Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr Lys 
            260                 265                 270         


Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln Leu 
        275                 280                 285             


Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp Pro 
    290                 295                 300                 


Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala Ala 
305                 310                 315                 320 


His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val Thr 
                325                 330                 335     


Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser Tyr 
            340                 345                 350         


Lys Lys Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln Pro 
        355                 360                 365             


Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile Ile 
    370                 375                 380                 


Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn Met 
385                 390                 395                 400 


Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly Ile 
                405                 410                 415     


Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp His 
            420                 425                 430         


Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val Asp 
        435                 440                 445             


Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser Asn 
    450                 455                 460                 


Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn Ile 
465                 470                 475                 480 


Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly Ser 
                485                 490                 495     


Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile Ala 
            500                 505                 510         


Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala Ile 
        515                 520                 525             


His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln Leu 
    530                 535                 540                 


Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn Leu 
545                 550                 555                 560 


Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu Leu 
                565                 570                 575     


Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp Gln 
            580                 585                 590         


Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr Glu 
        595                 600                 605             


Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala Lys 
    610                 615                 620                 


Lys Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn Arg 
625                 630                 635                 640 


Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu Ala 
                645                 650                 655     


Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala Leu 
            660                 665                 670         


Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu His 
        675                 680                 685             


Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg His 
    690                 695                 700                 


Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro Pro 
705                 710                 715                 720 


Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr Pro 
                725                 730                 735     


Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala Ser 
            740                 745                 750         


Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu Val 
        755                 760                 765             


Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys Ile 
    770                 775                 780                 


Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser Gly 
785                 790                 795                 800 


Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr Gln 
                805                 810                 815     


Arg 
    


<210>  13
<211>  818
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SPYAD [33-849 FRAGMENT] NNAA (E.G., PAMF) W/O LEADER, WITH G 
       OVERHANG FROM CLEAVAGE


<220>
<221>  SITE
<222>  (33)..(33)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (256)..(256)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (355)..(355)
<223>  Xaa is a non-natural amino acid

<220>
<221>  SITE
<222>  (626)..(626)
<223>  Xaa is a non-natural amino acid

<400>  13

Gly Gln Val Lys Ala Asp Asp Arg Ala Ser Gly Glu Thr Lys Ala Ser 
1               5                   10                  15      


Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu Thr Ile Gln Glu Ala 
            20                  25                  30          


Xaa Ala Thr Ile Asp Ala Val Glu Lys Thr Leu Ser Gln Gln Lys Ala 
        35                  40                  45              


Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys Thr Thr Ala Glu Ile 
    50                  55                  60                  


Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln Lys Ala Leu Thr Ser 
65                  70                  75                  80  


Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser Ser Glu Glu Thr Leu 
                85                  90                  95      


Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu Thr Ala Thr Glu Thr 
            100                 105                 110         


Glu Leu His Asn Ala Gln Ala Asp Gln His Ser Lys Glu Thr Ala Leu 
        115                 120                 125             


Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr Thr Arg Ala Gln Asp 
    130                 135                 140                 


Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn Ile Ala Lys Leu Asn 
145                 150                 155                 160 


Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys Ala Ala Gln Thr Ala 
                165                 170                 175     


Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu Glu Lys Ala Lys Ala 
            180                 185                 190         


Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys Gln Leu Thr Glu Glu 
        195                 200                 205             


Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys Glu Ala Glu Leu Ser 
    210                 215                 220                 


Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp Ser Ile Val Gly Asn 
225                 230                 235                 240 


Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu Glu Glu Leu Lys Xaa 
                245                 250                 255     


Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser Tyr Asn Asn Tyr Tyr 
            260                 265                 270         


Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala Ser Pro Gly Asn Gln 
        275                 280                 285             


Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg Asn Arg Phe Val Asp 
    290                 295                 300                 


Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu Leu Ala Gln Phe Ala 
305                 310                 315                 320 


Ala His Met Ile Asn Ser Val Arg Arg Gln Leu Gly Leu Pro Pro Val 
                325                 330                 335     


Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg Leu Leu Ser Thr Ser 
            340                 345                 350         


Tyr Lys Xaa Thr His Gly Asn Thr Arg Pro Ser Phe Val Tyr Gly Gln 
        355                 360                 365             


Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro His Asp Lys Thr Ile 
    370                 375                 380                 


Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile Arg Asn Asp Asp Asn 
385                 390                 395                 400 


Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val His Thr Val Asn Gly 
                405                 410                 415     


Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr Met Leu Phe Thr Asp 
            420                 425                 430         


His Leu His Gly Asn Thr Tyr Gly His Ala Ile Asn Phe Leu Arg Val 
        435                 440                 445             


Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu Gly Phe Ser Thr Ser 
    450                 455                 460                 


Asn Val Gly Ser Leu Asn Glu His Phe Val Met Phe Pro Glu Ser Asn 
465                 470                 475                 480 


Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro Ile Lys Ala Val Gly 
                485                 490                 495     


Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr Val Ser Asp Thr Ile 
            500                 505                 510         


Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu Asn Arg Leu Ser Ala 
        515                 520                 525             


Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln Ala Lys Val Ser Gln 
    530                 535                 540                 


Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln Ser Asp Ser Leu Asn 
545                 550                 555                 560 


Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly Ser Leu Arg Thr Glu 
                565                 570                 575     


Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu Glu Ala Thr Arg Asp 
            580                 585                 590         


Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala Ala Leu His Gln Thr 
        595                 600                 605             


Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val Thr Ala Leu Val Ala 
    610                 615                 620                 


Lys Xaa Ala His Leu Gln Tyr Leu Arg Asp Phe Lys Leu Asn Pro Asn 
625                 630                 635                 640 


Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn Thr Lys Gln Asp Leu 
                645                 650                 655     


Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln Glu Ala Leu Ala Ala 
            660                 665                 670         


Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr Ile Ala Thr Thr Glu 
        675                 680                 685             


His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn Glu Lys Glu Tyr Arg 
    690                 695                 700                 


His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp Leu Gln Val Ala Pro 
705                 710                 715                 720 


Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser Lys Ile Asp Thr Thr 
                725                 730                 735     


Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys Gln Leu Leu Glu Ala 
            740                 745                 750         


Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu Val Ala Glu Ala Leu 
        755                 760                 765             


Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn Ala Ile Val Ser Lys 
    770                 775                 780                 


Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys Thr Ser Tyr Gly Ser 
785                 790                 795                 800 


Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp Val Asp Glu Ser Thr 
                805                 810                 815     


Gln Arg 
        


