                         SEQUENCE LISTING

<110>  TechnoVax, Inc.
 
<120>  SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 
       [SARS-COV-2]-VIRUS-LIKE PARTICLE [VLP] VACCINE: COMPOSITIONS, 
       DELIVERY STRATEGIES, METHODS AND USES

<130>  10050-010184-WO0

<140>	PCT/US2021/046353
<141>  2021-08-17

<150>  63/066,617
<151>  2020-08-17

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Original S glycoprotein (Wuhan strain, MN988669)

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  2
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M from Wuhan strain (MN988669)

<400>  2

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      


Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          


Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              


Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  


Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  


Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      


Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         


Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             


Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 


Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 


Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     


Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         


Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             


Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         


<210>  3
<211>  75
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E from Wuhan strain (MN988669)

<400>  3

Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      


Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          


Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              


Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  


Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  


<210>  4
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N from Wuhan strain (MN988669)

<400>  4

Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 
1               5                   10                  15      


Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 
            20                  25                  30          


Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 
        35                  40                  45              


Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 
    50                  55                  60                  


Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 
65                  70                  75                  80  


Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 
                85                  90                  95      


Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr 
            100                 105                 110         


Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp 
        115                 120                 125             


Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp 
    130                 135                 140                 


His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln 
145                 150                 155                 160 


Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser 
                165                 170                 175     


Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn 
            180                 185                 190         


Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala 
        195                 200                 205             


Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu 
    210                 215                 220                 


Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln 
225                 230                 235                 240 


Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys 
                245                 250                 255     


Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln 
            260                 265                 270         


Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp 
        275                 280                 285             


Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile 
    290                 295                 300                 


Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile 
305                 310                 315                 320 


Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala 
                325                 330                 335     


Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 
            340                 345                 350         


Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 
        355                 360                 365             


Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 
    370                 375                 380                 


Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 
385                 390                 395                 400 


Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 
                405                 410                 415     


Thr Gln Ala 
            


<210>  5
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S glycoprotein with Triple mutations and S1-S2 mutation (Wuhan 
       strain, MN988669)

<400>  5

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Cys Glu His Val 
                645                 650                 655     


Asn Asn Cys Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ser Gly Ser Ala Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Cys Tyr Thr Cys Cys Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  6
<211>  1270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S glycoprotein from South Africa strain with mutations (B.1.351)

<400>  6

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 
                245                 250                 255     


Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 
            260                 265                 270         


Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 
        275                 280                 285             


Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 
    290                 295                 300                 


Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 
305                 310                 315                 320 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                325                 330                 335     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            340                 345                 350         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        355                 360                 365             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    370                 375                 380                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
385                 390                 395                 400 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile Ala 
                405                 410                 415     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            420                 425                 430         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        435                 440                 445             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    450                 455                 460                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
465                 470                 475                 480 


Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                485                 490                 495     


Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            500                 505                 510         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        515                 520                 525             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 
    530                 535                 540                 


Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 
545                 550                 555                 560 


Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro 
                565                 570                 575     


Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 
            580                 585                 590         


Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 
        595                 600                 605             


Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 
    610                 615                 620                 


Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 
625                 630                 635                 640 


Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 
                645                 650                 655     


Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 
            660                 665                 670         


Thr Gln Thr Asn Ser Pro Ser Gly Ser Ala Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  7
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S glycoprotein from India strain with mutations ((B .1. 617)

<400>  7

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Ile Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Lys Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Gln Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Arg Ser Gly Ser Ala Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  His Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  8
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Original S glycoprotein (Wuhan strain, MN988669)

<400>  8
atgttcgtgt tcctcgtgct gctgcccctg gtgtctagcc agtgcgtgaa cctgaccacc       60

agaacccagc tgcctccagc ctacaccaac agcttcacca ggggagtgta ctaccccgac      120

aaggtgttcc ggagcagcgt gctgcatagc acccaggacc tgttcctgcc cttcttcagc      180

aacgtgactt ggttccacgc catccacgtc agcggaacca acggcaccaa gcgctttgac      240

aacccagtgc tgcccttcaa cgacggagtg tacttcgcca gcaccgagaa gagcaacatc      300

atccggggtt ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtcgt gatcaaggtc tgcgagttcc agttttgcaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt ccgggtgtac      480

agcagcgcca acaattgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccccatc aacctcgtga gagacctgcc acagggcttc      660

agcgctctgg aacctctggt ggatctgcca atcggcatca acatcacccg gttccagacc      720

ctgctggctc tgcacagaag ctacctgacc ccaggcgatt cttctagcgg ctggacagca      780

ggagccgccg cttattacgt gggctatctg cagccccgga ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgcagtggat tgcgctctgg atcctctgag cgagaccaag      900

tgcaccctga agagcttcac cgtggagaag ggcatctacc agaccagcaa cttccgggtg      960

cagcctacag agagcatcgt gcggttccct aacatcacca acctctgccc cttcggcgag     1020

gtgttcaacg ccaccagatt cgccagcgtc tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actacagcgt gctgtacaac agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgatctg tgcttcacca acgtgtacgc cgacagcttc     1200

gtgatcaggg gcgacgaagt gcgacagatc gccccaggac agacaggaaa gatcgccgat     1260

tacaactaca agctgcccga cgacttcacc ggctgcgtga tcgcttggaa cagcaacaac     1320

ctggacagca aggtcggcgg caactacaac tacctgtacc ggctgttccg gaagagcaac     1380

ctgaagccct tcgagcggga catcagcaca gagatctacc aggccggcag cacaccttgt     1440

aacggcgtgg agggcttcaa ctgctacttc cctctgcaga gctacggctt ccagcctaca     1500

aacggcgtgg gataccagcc ttacagggtg gtggtgctga gcttcgaact gctgcacgct     1560

ccagccaccg tctgcggacc taaaaagagc accaacctgg tgaagaacaa gtgcgtgaat     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggcag agacatcgca gacaccacag acgccgtgag agatcctcag     1740

accctggaga tcctggacat caccccttgc agctttggcg gagtgtccgt gatcacccca     1800

ggaaccaaca ccagcaatca ggtggccgtg ctgtaccagg acgtgaattg caccgaggtg     1860

ccagtggcca ttcacgcaga tcagctgacc cctacctgga gagtgtacag caccggaagc     1920

aacgtgttcc agaccagggc aggttgcctg atcggagccg agcacgtgaa caacagctac     1980

gagtgcgaca tccccatcgg cgccggcatt tgcgcctctt accagaccca gaccaacagc     2040

cctagaagag ccagaagcgt ggcctctcag agcatcatcg cctacaccat gagcctggga     2100

gccgagaaca gcgtggccta cagcaacaac agcatcgcca tccccaccaa cttcaccatc     2160

agcgtgacca ccgagattct gcccgtgtct atgaccaaga ccagcgtcga ctgcaccatg     2220

tacatctgcg gcgacagcac cgagtgctct aacctgctgc tgcagtacgg ctccttttgc     2280

acccagctga acagggctct gacaggcatc gcagtggagc aggacaagaa cacccaggag     2340

gtcttcgccc aggtcaagca gatctacaag acccccccta tcaaggactt cggcggcttt     2400

aacttcagcc agatcctgcc cgatcccagc aagcctagca agcggagctt catcgaggac     2460

ctgctgttca acaaggtcac cctggccgac gcaggcttca tcaagcagta cggcgattgc     2520

ctgggcgaca ttgccgccag agacctgatt tgcgcccaga agttcaacgg cctgacagtg     2580

ctgcctcctc tgctgaccga cgagatgatc gcccagtata caagcgctct gctggcagga     2640

accatcacca gcggttggac ctttggagcc ggagccgctc tgcagatccc ttttgccatg     2700

cagatggcct accgcttcaa cggaatcggc gtgacccaga acgtgctcta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagctagcg ctctgggcaa gctgcaggat gtggtgaacc agaacgccca ggctctgaac     2880

accctggtga agcagctgag cagcaacttc ggagccatca gcagcgtgct gaacgacatc     2940

ctgagcaggc tggacaaggt ggaagccgaa gtgcagatcg acaggctgat caccggcaga     3000

ctgcagtctc tgcagaccta cgtgacccag cagctgatca gagccgccga gatcagagct     3060

agcgctaatc tggccgccac caagatgagc gagtgcgtgc tgggacagag caagagagtg     3120

gacttctgcg gcaagggcta ccacctgatg agctttcctc agagcgctcc tcacggagtg     3180

gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agccccagcc     3240

atttgccacg acggaaaggc ccacttcccc agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacccagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgagcg gcaattgcga cgtggtcatc ggcatcgtga acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc cttcaaggag gagctggaca agtacttcaa gaaccacacc     3480

agcccagacg tggacctggg agacatcagc ggcatcaacg ccagcgtggt gaacatccag     3540

aaggagatcg acaggctgaa cgaggtggcc aagaacctga acgagagcct gatcgacctg     3600

caggagctgg gcaagtacga gcagtacatc aagtggcctt ggtacatttg gctgggcttc     3660

atcgccggcc tgatcgcaat cgtcatggtg accatcatgc tctgttgcat gaccagttgc     3720

tgcagctgcc tgaagggttg ctgcagttgc gggagttgct gcaagttcga cgaggacgac     3780

agcgagccag tgctgaaagg cgtgaagctg cactacacct aa                        3822


<210>  9
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  M protein from Wuhan strain (MN988669)

<400>  9
atggccgact ctaacggcac catcaccgtg gaggagctga agaagctgct cgagcagtgg       60

aacctggtca tcggcttcct gttcctcacc tggatttgcc tgctgcagtt cgcctacgcc      120

aaccggaacc ggttcctgta catcatcaag ctgatcttcc tctggctcct ctggcccgtg      180

acactggctt gtttcgtgct ggcagccgtg taccggatca attggatcac cggcggcatc      240

gctatcgcta tggcttgtct cgtgggcctg atgtggctga gctacttcat cgccagcttc      300

cggctgttcg ccagaaccag aagcatgtgg agcttcaacc ccgagaccaa catcctgctg      360

aacgtgcctc tgcacggcac aatcctgacc agacctctgc tggagagcga gctggtcatc      420

ggagcagtga tcctgagagg ccacctgaga atcgccggac atcatctggg acggtgcgac      480

atcaaggacc tgcccaagga gatcacagtg gccacaagcc ggaccctgag ctactacaag      540

ctgggagcca gccagagagt ggcaggagat agcggattcg ccgcctacag cagatacagg      600

atcggcaact acaagctgaa caccgaccac agcagcagca gcgataacat cgccctgctg      660

gtgcagtaa                                                              669


<210>  10
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E protein from Wuhan strain (MN988669)

<400>  10
atgtacagct tcgtgtccga ggagaccgga accctgatcg tgaactccgt gctgctgttc       60

ctggcctttg tggtgttcct gctggtgacc ctggccattc tgacagctct caggctctgc      120

gcctattgct gcaacatcgt gaacgtgtcc ctggtgaagc ccagcttcta cgtgtacagc      180

cgcgtgaaga acctgaacag cagcagggtg ccagatctgc tggtgtaa                   228


<210>  11
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N protein from Wuhan strain (MN988669)

<400>  11
atgagcgaca acggacctca gaaccagcgg aacgccccta gaatcacctt tggcggccct       60

agcgatagca ccggcagcaa tcagaacggc gagagaagcg gcgccagatc taagcagagg      120

agacctcagg gcctgcctaa caataccgcc tcttggttca ccgctctgac acagcacggc      180

aaggaggacc tgaagttccc taggggccag ggcgtgccta tcaataccaa cagcagcccc      240

gacgatcaga tcggctacta caggagggcc accagaagga tcagaggcgg agacggcaag      300

atgaaggacc tgagcccccg ctggtacttt tactatctgg gcacaggacc agaggccgga      360

ctgccttacg gagccaacaa ggacggcatc atttgggtgg ccaccgaagg agccctgaac      420

acccctaagg accacatcgg caccaggaac ccagccaata acgccgctat cgtgctgcag      480

ctgcctcagg gaacaacact gcctaagggc ttttacgccg agggcagcag aggaggaagc      540

caggcctcta gcagaagcag cagcagaagc aggaacagca gcagaaacag caccccaggc      600

agcagcagag gaaccagccc agccagaatg gcaggaaacg gaggagacgc agctctggct      660

ctgctgctgc tggataggct gaaccagctg gagagcaaga tgagcggcaa gggacagcag      720

cagcagggac agaccgtgac caagaagagc gccgccgaag cctctaagaa gcctagacag      780

aagcggaccg ccaccaaggc ttacaacgtg acccaggcct tcggcagaag aggaccagaa      840

cagacccagg gcaacttcgg cgatcaggaa ctgatccggc agggcaccga ctacaagcat      900

tggcctcaga tcgcccagtt tgctcctagc gccagcgcct ttttcggcat gagcaggatc      960

ggcatggaag tgacccctag cggaacttgg ctgacctaca ccggagccat caagctggac     1020

gacaaggacc ccaacttcaa ggaccaggtc atcctgctga acaagcacat cgacgcctac     1080

aagaccttcc ctcccaccga gcccaagaag gacaagaaga agaaggccga cgagacccag     1140

gctctgcctc agaggcagaa gaagcagcag accgtgacac tgctgccagc agcagatctg     1200

gacgacttca gcaagcagct gcagcagagc atgagcagcg ccgattctac acaggcctaa     1260


<210>  12
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S glycoprotein with Triple mutations and S1-S2 mutation (Wuhan 
       strain, MN988669)

<400>  12
atgttcgtgt tcctcgtgct gctgcccctg gtgtctagcc agtgcgtgaa cctgaccacc       60

agaacccagc tgcctccagc ctacaccaac agcttcacca ggggagtgta ctaccccgac      120

aaggtgttcc ggagcagcgt gctgcatagc acccaggacc tgttcctgcc cttcttcagc      180

aacgtgactt ggttccacgc catccacgtc agcggaacca acggcaccaa gcgctttgac      240

aacccagtgc tgcccttcaa cgacggagtg tacttcgcca gcaccgagaa gagcaacatc      300

atccggggtt ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtcgt gatcaaggtc tgcgagttcc agttttgcaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt ccgggtgtac      480

agcagcgcca acaattgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccccatc aacctcgtga gagacctgcc acagggcttc      660

agcgctctgg aacctctggt ggatctgcca atcggcatca acatcacccg gttccagacc      720

ctgctggctc tgcacagaag ctacctgacc ccaggcgatt cttctagcgg ctggacagca      780

ggagccgccg cttattacgt gggctatctg cagccccgga ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgcagtggat tgcgctctgg atcctctgag cgagaccaag      900

tgcaccctga agagcttcac cgtggagaag ggcatctacc agaccagcaa cttccgggtg      960

cagcctacag agagcatcgt gcggttccct aacatcacca acctctgccc cttcggcgag     1020

gtgttcaacg ccaccagatt cgccagcgtc tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actacagcgt gctgtacaac agcgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgatctg tgcttcacca acgtgtacgc cgacagcttc     1200

gtgatcaggg gcgacgaagt gcgacagatc gccccaggac agacaggaaa gatcgccgat     1260

tacaactaca agctgcccga cgacttcacc ggctgcgtga tcgcttggaa cagcaacaac     1320

ctggacagca aggtcggcgg caactacaac tacctgtacc ggctgttccg gaagagcaac     1380

ctgaagccct tcgagcggga catcagcaca gagatctacc aggccggcag cacaccttgt     1440

aacggcgtgg agggcttcaa ctgctacttc cctctgcaga gctacggctt ccagcctaca     1500

aacggcgtgg gataccagcc ttacagggtg gtggtgctga gcttcgaact gctgcacgct     1560

ccagccaccg tctgcggacc taaaaagagc accaacctgg tgaagaacaa gtgcgtgaat     1620

ttcaacttca acggcctgac cggaaccggc gtgctgaccg agagcaacaa gaagttcctg     1680

cccttccagc agttcggcag agacatcgca gacaccacag acgccgtgag agatcctcag     1740

accctggaga tcctggacat caccccttgc agctttggcg gagtgtccgt gatcacccca     1800

ggaaccaaca ccagcaatca ggtggccgtg ctgtaccagg acgtgaattg caccgaggtg     1860

ccagtggcca ttcacgcaga tcagctgacc cctacctgga gagtgtacag caccggaagc     1920

aacgtgttcc agaccagggc aggttgcctg atcggatgcg agcacgtgaa caactgctac     1980

gagtgcgaca tccccatcgg cgccggcatt tgcgcctctt accagaccca gaccaacagc     2040

cctagtggta gcgctagcgt ggcctctcag agcatcatct gctacacctg ctgcctggga     2100

gccgagaaca gcgtggccta cagcaacaac agcatcgcca tccccaccaa cttcaccatc     2160

agcgtgacca ccgagattct gcccgtgtct atgaccaaga ccagcgtcga ctgcaccatg     2220

tacatctgcg gcgacagcac cgagtgctct aacctgctgc tgcagtacgg ctccttttgc     2280

acccagctga acagggctct gacaggcatc gcagtggagc aggacaagaa cacccaggag     2340

gtcttcgccc aggtcaagca gatctacaag acccccccta tcaaggactt cggcggcttt     2400

aacttcagcc agatcctgcc cgatcccagc aagcctagca agcggagctt catcgaggac     2460

ctgctgttca acaaggtcac cctggccgac gcaggcttca tcaagcagta cggcgattgc     2520

ctgggcgaca ttgccgccag agacctgatt tgcgcccaga agttcaacgg cctgacagtg     2580

ctgcctcctc tgctgaccga cgagatgatc gcccagtata caagcgctct gctggcagga     2640

accatcacca gcggttggac ctttggagcc ggagccgctc tgcagatccc ttttgccatg     2700

cagatggcct accgcttcaa cggaatcggc gtgacccaga acgtgctcta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagctagcg ctctgggcaa gctgcaggat gtggtgaacc agaacgccca ggctctgaac     2880

accctggtga agcagctgag cagcaacttc ggagccatca gcagcgtgct gaacgacatc     2940

ctgagcaggc tggacaaggt ggaagccgaa gtgcagatcg acaggctgat caccggcaga     3000

ctgcagtctc tgcagaccta cgtgacccag cagctgatca gagccgccga gatcagagct     3060

agcgctaatc tggccgccac caagatgagc gagtgcgtgc tgggacagag caagagagtg     3120

gacttctgcg gcaagggcta ccacctgatg agctttcctc agagcgctcc tcacggagtg     3180

gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agccccagcc     3240

atttgccacg acggaaaggc ccacttcccc agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacccagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgagcg gcaattgcga cgtggtcatc ggcatcgtga acaacaccgt gtacgacccc     3420

ctgcagccag agctggactc cttcaaggag gagctggaca agtacttcaa gaaccacacc     3480

agcccagacg tggacctggg agacatcagc ggcatcaacg ccagcgtggt gaacatccag     3540

aaggagatcg acaggctgaa cgaggtggcc aagaacctga acgagagcct gatcgacctg     3600

caggagctgg gcaagtacga gcagtacatc aagtggcctt ggtacatttg gctgggcttc     3660

atcgccggcc tgatcgcaat cgtcatggtg accatcatgc tctgttgcat gaccagttgc     3720

tgcagctgcc tgaagggttg ctgcagttgc gggagttgct gcaagttcga cgaggacgac     3780

agcgagccag tgctgaaagg cgtgaagctg cactacacct aa                        3822


<210>  13
<211>  3813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S glycoprotein from South Africa strain with mutations (B.1.351)

<400>  13
atgttcgtgt tcctcgtgct gctgcccctg gtgtctagcc agtgcgtgaa cctgaccacc       60

agaacccagc tgcctccagc ctacaccaac agcttcacca ggggagtgta ctaccccgac      120

aaggtgttcc ggagcagcgt gctgcatagc acccaggacc tgttcctgcc cttcttcagc      180

aacgtgactt ggttccacgc catccacgtg tccggaacca acggcaccaa gagattcgcc      240

aacccagtgc tgcccttcaa cgacggagtg tacttcgcca gcaccgagaa gagcaacatc      300

atccggggtt ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtcgt gatcaaggtc tgcgagttcc agttttgcaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt ccgggtgtac      480

agcagcgcca acaattgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccccatc aacctcgtga gaggcctgcc tcagggattc      660

tcagccctgg aacctctggt ggatctgcca atcggcatca acatcacccg gttccagacc      720

ctgcacagaa gctacctgac cccaggcgat tcttctagcg gctggacagc aggagccgca      780

gcttactacg tgggatacct gcagcccagg accttcctgc tgaagtacaa cgagaacggc      840

accatcaccg acgcagtgga ttgcgctctg gaccctctga gcgagaccaa gtgtaccctc      900

aagagcttca ccgtggagaa gggcatctac cagaccagca acttccgggt gcagcctaca      960

gagagcatcg tgcggttccc caacatcacc aacctctgcc ccttcggcga ggtgttcaac     1020

gctaccagat tcgccagcgt gtacgcttgg aaccggaagc ggatcagcaa ttgcgtggcc     1080

gactacagcg tgctgtacaa cagcgccagc ttcagcacct tcaagtgcta cggcgtgtct     1140

cccaccaagc tgaacgacct ctgcttcacc aacgtgtacg ccgacagctt cgtgatccgg     1200

ggagacgaag tgcgccagat tgctccagga cagaccggca acatcgccga ctacaactac     1260

aagctgcccg acgacttcac cggctgcgtg atcgcctgga acagcaacaa cctggacagc     1320

aaggtcggcg gcaactacaa ctacctgtac cggctgttcc ggaagagcaa cctgaagcct     1380

ttcgagcggg acatcagcac cgagatctac caggcaggca gcacaccttg taacggagtg     1440

aagggcttca actgctactt ccccctgcag agctacggct ttcagcctac ctacggcgtg     1500

ggatatcagc cctacagggt ggtggtgctg agctttgagc tgctgcacgc cccagctaca     1560

gtgtgcggac ccaagaagag caccaacctg gtgaagaaca agtgcgtgaa cttcaacttc     1620

aatggcctga ccggcacagg agtgctgaca gagagcaaca agaagttcct gcccttccag     1680

cagttcggca gggatatcgc cgataccacc gacgccgtga gagatcctca gacactggag     1740

atcctggaca tcaccccttg cagcttcgga ggagtgtccg tgatcacccc aggcacaaac     1800

accagcaacc aggtggcagt gctgtaccag ggcgtgaatt gcaccgaagt gccagtggct     1860

atccacgcag accagctgac ccctacttgg agggtgtaca gcaccggcag caacgtgttc     1920

cagaccagag caggttgtct gatcggagcc gagcacgtga acaacagcta cgagtgcgac     1980

atccctatcg gagccggcat ttgcgcctct taccagaccc agaccaactc tcctagcggc     2040

agcgccagcg tggcctctca gtctatcatc gcctacacca tgagcctggg cgtggaaaac     2100

agcgtggcct acagcaacaa cagcatcgcc atccccacca acttcaccat cagcgtgacc     2160

accgagatcc tgccagtgtc catgaccaag accagcgtcg actgcaccat gtacatttgc     2220

ggcgatagca ccgagtgctc caatctgctg ctgcagtacg gctccttttg cacccagctg     2280

aacagagccc tgacaggaat cgccgtggag caggacaaga acacccagga ggtcttcgcc     2340

caggtcaagc agatctacaa gacccccccc atcaaggact tcggcggctt caacttcagc     2400

cagatcctgc cagatcccag caagcctagc aagcggagct tcatcgagga cctgctgttc     2460

aacaaggtca cactggccga cgccggcttt atcaagcagt acggcgattg cctgggcgac     2520

attgcagcca gggacctgat ttgcgcccag aagttcaacg gcctgaccgt gctgcctcct     2580

ctgctgaccg acgagatgat cgcccagtac acaagcgccc tgctggccgg aacaatcaca     2640

agcggatgga catttggagc cggagcagct ctgcagatcc ctttcgccat gcagatggcc     2700

taccgcttca acggaatcgg cgtgacccag aacgtgctct acgagaacca gaagctgatc     2760

gccaaccagt tcaacagcgc catcggcaag atccaggaca gcctgtctag cacagccagc     2820

gctctgggaa agctgcagga cgtggtgaac cagaacgctc aggccctgaa taccctggtg     2880

aagcagctga gcagcaactt cggagccatc agcagcgtgc tgaacgacat cctgagcagg     2940

ctggaccctc cagaagccga agtgcagatc gacaggctga tcaccggcag actgcagtct     3000

ctgcagacct acgtgaccca gcagctgatc agagccgcag agatcagagc cagcgccaat     3060

ctggcagcta ccaagatgag cgagtgcgtc ctgggacaga gcaagcgggt ggacttttgc     3120

ggcaagggct accacctgat gagcttccct cagagcgctc ctcacggagt ggtgtttctg     3180

cacgtgacct acgtgccagc ccaggaaaag aacttcacca ccgccccagc catttgccac     3240

gacggaaagg cccactttcc tagagagggc gtgttcgtgt ccaacggcac ccattggttc     3300

gtgacccagc ggaacttcta cgagccccag atcatcacca ccgacaacac cttcgtgagc     3360

ggcaattgcg acgtggtcat cggcatcgtg aacaacaccg tgtacgaccc cctgcagcca     3420

gagctggact ccttcaagga ggagctggac aagtacttca agaaccacac cagcccagac     3480

gtggacctgg gcgatatcag cggcatcaac gccagcgtgg tgaacatcca gaaggagatc     3540

gacaggctga acgaggtggc caagaacctg aacgagagcc tgatcgacct gcaggagctg     3600

ggcaagtacg agcagtacat caagtggcct tggtacattt ggctgggctt catcgccggc     3660

ctgatcgcca tcgtcatggt caccatcatg ctctgctgca tgaccagttg ctgctcttgc     3720

ctgaagggtt gctgcagctg cgggtcttgt tgcaagttcg acgaggacga cagcgagcca     3780

gtgctgaagg gcgtgaagct gcactacacc taa                                  3813


<210>  14
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S glycoprotein from India strain with mutations ((B .1. 617)

<400>  14
atgttcgtgt tcctcgtgct gctgcccctc gttagttctc aatgcgtgaa cctgaccacg       60

cggacccagc tgccacccgc ctataccaac tcatttactc gaggggtgta ctatcccgat      120

aaggtgtttc gatcatctgt actccacagc acccaagatc tgtttctccc attcttcagt      180

aacgtgacct ggtttcacgc cattcacgtg agtggcacta acggcacaaa acgcttcgac      240

aatccggtgc tgcctttcaa cgacggcgta tacttcgcat caatagagaa aagcaatatc      300

atccggggtt ggatattcgg cacaaccctg gatagcaaaa cacaaagtct gctcatcgtc      360

aacaatgcga cgaacgtagt gatcaaggtg tgcgagttcc agttctgcaa cgaccccttc      420

ctggacgtgt actatcataa gaataataag tcctggatga agtcagaatt tcgggtgtac      480

tcaagcgcta acaactgtac cttcgagtac gtgtcacaac cattcctcat ggatctcgag      540

ggcaagcaag gaaactttaa gaacttgaga gagttcgttt tcaagaacat cgacggctac      600

ttcaagattt acagtaaaca tacaccaatc aacctggtta gagacctgcc acaaggcttc      660

tctgccctcg agcccctcgt cgacctgccc atcgggatca atattacgcg tttccagaca      720

ctcctggccc tgcacaggtc atacctcaca cccggcgact catcatccgg gtggactgca      780

ggagccgctg cctactacgt aggatacctg cagccccgca ccttcctgct gaagtacaac      840

gagaacggca ctataaccga cgccgtggat tgcgctctcg atcccctttc tgagacgaaa      900

tgcactctca agtcatttac cgtcgagaag ggcatttacc agaccagcaa tttcagggtt      960

cagcctactg agtcaatcgt gcgattccca aacatcacga atctgtgtcc gttcggggaa     1020

gtcttcaatg cgactcggtt cgccagcgtg tacgcctgga atagaaaacg tattagtaat     1080

tgcgtggcag actactccgt gctttacaac agcgctagtt tctctacatt caaatgctac     1140

ggggtaagtc ccacaaagtt gaacgacttg tgtttcacaa acgtatacgc ggacagcttc     1200

gtgatccgtg gcgacgaggt gcgccagatt gcccccggtc agacagggaa aatagccgac     1260

tacaactaca agcttcccga cgacttcacg ggttgtgtga ttgcctggaa ctcaaataac     1320

ctcgacagca aagtcggtgg gaactacaac tataggtaca ggctgttccg caaatcaaac     1380

ctgaagccct tcgaacgcga catcagtacc gagatatacc aagctggatc tactccatgc     1440

aacggcgtcc agggcttcaa ctgctatttc cccctgcaga gttacgggtt tcagcctaca     1500

aacggagtgg gatatcagcc ttatcgggtg gtcgtcctga gtttcgagct gcttcacgct     1560

cctgcgaccg tctgcggccc caagaaatca acgaaccttg taaagaataa gtgcgttaac     1620

tttaatttta acggcctgac cgggactggg gtgttgacag aatccaataa gaaattcctt     1680

ccatttcagc agttcggtag ggatatagca gataccaccg acgccgtgag ggaccctcaa     1740

acgctcgaaa tcctcgatat caccccctgc tcattcggcg gggtttccgt catcaccccc     1800

gggaccaaca cctcaaatca agtggccgtg ctgtaccaag gggtcaattg tactgaggtt     1860

ccggtggcaa tccacgccga ccagctgacc ccaacctgga gagtgtactc cactggcagt     1920

aacgtcttcc agacccgcgc tggttgcctc atcggcgccg agcacgtgaa taattcctac     1980

gaatgcgata tccctatcgg agccgggatc tgtgcatcat accaaacgca aaccaacagc     2040

agaagcggca gtgcatccgt ggcatctcag agcataatcg cgtatacaat gagtttgggc     2100

gccgagaact ctgtcgcata ttccaacaat tccatcgcga tccctacgaa cttcaccatc     2160

tctgtcacaa ccgagatact ccctgtatca atgactaaaa ctagtgtgga ctgcaccatg     2220

tatatctgcg gcgacagcac cgagtgttct aacctgctgc tgcagtacgg aagcttctgc     2280

actcagttga atcgagccct gacgggcatt gccgtggagc aggataagaa tacgcaggaa     2340

gtcttcgctc aggtgaagca gatctataag actcccccta tcaaggactt cggcggcttc     2400

aacttctctc agattttgcc cgacccttcc aagccttcaa aacgcagctt catcgaggac     2460

ctgttgttta ataaggttac cctggccgac gccggattta ttaagcagta cggggactgt     2520

ctcggggaca tcgcagcaag ggatctgata tgcgcccaga aattcaatgg gttgacagta     2580

cttcccccgc tgcttactga cgagatgatc gcccagtata cgagcgcgct cctggccggg     2640

acgattacat cagggtggac tttcggcgct ggggcagccc tgcagattcc cttcgccatg     2700

cagatggcct accgcttcaa cggcatcggc gtgacccaaa acgtgctgta cgaaaatcag     2760

aagctgatcg caaatcagtt caactccgcc atagggaaga tccaggatag cttgagctca     2820

accgcctctg ctctcgggaa gctccaggac gtcgtgaatc agaacgctca ggccctcaat     2880

accctggtca agcagctcag tagcaacttc ggcgctatct cttctgtgtt gaacgacatt     2940

ctgagcaggc tggatccacc ggaagcagag gtccagatag accgactgat tactggaagg     3000

ctgcagagcc ttcaaactta cgtaacccag cagcttatca gggcagccga gataagggcc     3060

agcgccaact tggccgcgac gaagatgtct gaatgcgttt tgggccagtc taagagggtg     3120

gacttctgcg ggaaagggta ccacttgatg tcatttcccc aaagcgcccc acacggagtg     3180

gtgtttcttc acgtcaccta cgtgcccgct cacgagaaga attttaccac agcgcccgct     3240

atatgccacg acggtaaggc ccatttcccc agagagggcg tgttcgtgag caacgggact     3300

cattggttcg tcacgcagcg caacttctac gagccccaga ttatcaccac tgataatact     3360

ttcgtcagcg gcaattgcga cgtggttatt ggcatcgtaa ataatactgt gtacgacccg     3420

ctgcagcccg agctcgattc ctttaaagaa gaactcgaca agtacttcaa gaaccacact     3480

agccccgacg tcgacctcgg agatatttcc gggataaacg ccagcgtcgt gaatatacag     3540

aaggagatcg atagactgaa cgaagtcgct aagaacctga acgagagcct tattgacctg     3600

caggagctcg gtaaatacga acaatacatt aagtggcctt ggtatatatg gctcggcttc     3660

atcgcgggac tgatcgctat cgtcatggtt actatcatgc tgtgttgcat gacttcatgt     3720

tgctcatgcc ttaaaggatg ctgctcctgc gggagctgtt gtaagttcga cgaggatgat     3780

tcagagccgg tattgaaagg ggtaaagctc cactatactt ga                        3822


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A653C

<400>  15

Leu Ile Gly Ala Glu His Val 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A694C

<400>  16

Ser Ile Ile Ala Tyr Thr Met 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S659C

<400>  17

Val Asn Asn Ser Tyr Glu Cys 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S698C

<400>  18

Tyr Thr Met Ser Leu Gly Ala 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C662C

<400>  19

Ser Tyr Glu Cys Asp Ile Pro 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M697C

<400>  20

Ala Tyr Thr Met Ser Leu Gly 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A570C

<400>  21

Arg Asp Ile Ala Asp Thr Thr 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V963C

<400>  22

Asn Thr Leu Val Lys Gln Leu 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  D571C

<400>  23

Asp Ile Ala Asp Thr Thr Asp 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S967C

<400>  24

Lys Gln Leu Ser Ser Asn Phe 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  K558C

<400>  25

Ser Asn Lys Lys Phe Leu Pro 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N282C

<400>  26

Tyr Asn Glu Asn Gly Thr Ile 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P862C

<400>  27

Thr Val Leu Pro Pro Leu Leu 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A668C

<400>  28

Pro Ile Gly Ala Gly Ile Cys 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Y707C

<400>  29

Ser Val Ala Tyr Ser Asn Asn 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T883C

<400>  30

Gly Thr Ile Thr Ser Gly Trp 
1               5           


<210>  31
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V705C

<400>  31

Glu Asn Ser Val Ala Tyr Ser 
1               5           


