                         SEQUENCE LISTING

<110>  Pictor Limited
 
<120>  MULTIPLEX ASSAY FOR JOHNE'S DISEASE AND PREGNANCY

<130>  PIC1004/PC

<140>  NZ767333
<141>  2020-08-20

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide Antigen 1

<400>  1

Asp Thr Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln 
1               5                   10              


<210>  2
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide Antigen 2

<400>  2

Gly Ser Val Val Met Phe Gly Gly Val Asp His Arg Tyr Tyr Lys Gly 
1               5                   10                  15      


Glu Leu Asn Trp 
            20  


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide Antigen 3

<400>  3

Ile Thr Ile Gly Thr Pro Pro Gln Glu Phe Gln Val 
1               5                   10          


<210>  4
<211>  379
<212>  PRT
<213>  Bos taurus

<400>  4

Met Lys Trp Ile Val Leu Leu Gly Leu Val Ala Phe Ser Glu Cys Ile 
1               5                   10                  15      


Val Lys Ile Pro Leu Arg Gln Val Lys Thr Met Arg Lys Thr Leu Ser 
            20                  25                  30          


Gly Lys Asn Met Leu Lys Asn Phe Leu Lys Glu His Pro Tyr Arg Leu 
        35                  40                  45              


Ser Gln Ile Ser Phe Arg Gly Ser Asn Leu Thr Ile His Pro Leu Arg 
    50                  55                  60                  


Asn Ile Met Asn Leu Val Tyr Val Gly Asn Ile Thr Ile Gly Thr Pro 
65                  70                  75                  80  


Pro Gln Glu Phe Gln Val Val Phe Asp Thr Gly Ser Ser Asp Leu Trp 
                85                  90                  95      


Val Pro Ser Phe Cys Thr Met Pro Ala Cys Ser Ala Pro Val Trp Phe 
            100                 105                 110         


Arg Gln Leu Gln Ser Ser Thr Phe Gln Pro Thr Asn Lys Thr Phe Thr 
        115                 120                 125             


Ile Thr Tyr Gly Ser Gly Ser Met Lys Gly Phe Leu Ala Tyr Asp Thr 
    130                 135                 140                 


Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln Pro Phe Gly Leu Ser 
145                 150                 155                 160 


Val Val Glu Tyr Gly Leu Glu Gly Arg Asn Tyr Asp Gly Val Leu Gly 
                165                 170                 175     


Leu Asn Tyr Pro Asn Ile Ser Phe Ser Gly Ala Ile Pro Ile Phe Asp 
            180                 185                 190         


Asn Leu Lys Asn Gln Gly Ala Ile Ser Glu Pro Val Phe Ala Phe Tyr 
        195                 200                 205             


Leu Ser Lys Asn Lys Gln Glu Gly Ser Val Val Met Phe Gly Gly Val 
    210                 215                 220                 


Asp His Gln Tyr Tyr Lys Gly Glu Leu Asn Trp Ile Pro Leu Ile Glu 
225                 230                 235                 240 


Ala Gly Glu Trp Arg Val His Met Asp Arg Ile Ser Met Lys Arg Thr 
                245                 250                 255     


Val Ile Ala Cys Ser Asp Gly Cys Glu Ala Leu Val His Thr Gly Thr 
            260                 265                 270         


Ser His Ile Glu Gly Pro Gly Arg Leu Val Asn Asn Ile His Arg Leu 
        275                 280                 285             


Ile Arg Thr Arg Pro Phe Asp Ser Lys His Tyr Val Ser Cys Phe Ala 
    290                 295                 300                 


Thr Lys Tyr Leu Pro Ser Ile Thr Phe Ile Ile Asn Gly Ile Lys Tyr 
305                 310                 315                 320 


Pro Met Thr Ala Arg Ala Tyr Ile Phe Lys Asp Ser Arg Gly Arg Cys 
                325                 330                 335     


Tyr Ser Ala Phe Lys Glu Asn Thr Val Arg Thr Ser Arg Glu Thr Trp 
            340                 345                 350         


Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Phe Ser Val Phe Asp Arg 
        355                 360                 365             


Gly Asn Asp Arg Ile Gly Leu Ala Arg Ala Val 
    370                 375                 


<210>  5
<211>  380
<212>  PRT
<213>  Bos taurus

<400>  5

Met Lys Trp Leu Val Leu Leu Gly Leu Val Ala Phe Ser Glu Cys Ile 
1               5                   10                  15      


Phe Lys Ile Pro Leu Arg Arg Val Lys Thr Met Arg Lys Thr Leu Ser 
            20                  25                  30          


Gly Lys Asn Met Leu Asn Asn Phe Leu Lys Glu His Pro Tyr Lys Leu 
        35                  40                  45              


Ser Gln Ile Ser Phe Arg Gly Ser Asn Leu Thr Thr Leu Pro Leu Arg 
    50                  55                  60                  


Asn Ile Trp Asp Ile Phe Tyr Ile Gly Thr Ile Thr Ile Gly Thr Pro 
65                  70                  75                  80  


Pro Gln Glu Phe Gln Val Val Phe Asp Thr Ala Ser Ser Asp Leu Trp 
                85                  90                  95      


Val Pro Ser Ile Ile Cys Asn Ser Ser Thr Cys Ser Thr His Val Arg 
            100                 105                 110         


Phe Arg His Arg Gln Ser Ser Thr Phe Arg Leu Thr Asn Lys Thr Phe 
        115                 120                 125             


Gly Ile Thr Tyr Gly Ser Gly Arg Met Lys Gly Val Val Val His Asp 
    130                 135                 140                 


Thr Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln Pro Phe Gly Leu 
145                 150                 155                 160 


Ser Val Ala Glu Tyr Gly Phe Glu Gly Arg Arg Phe Asp Gly Val Leu 
                165                 170                 175     


Gly Leu Asn Tyr Pro Asn Ile Ser Phe Ser Lys Ala Ile Pro Ile Phe 
            180                 185                 190         


Asp Lys Leu Lys Asn Glu Gly Ala Ile Ser Glu Pro Val Phe Ala Phe 
        195                 200                 205             


Tyr Leu Ser Lys Asp Lys Gln Lys Gly Ser Val Val Met Phe Gly Gly 
    210                 215                 220                 


Val Asp His Arg Tyr Tyr Lys Gly Glu Leu Asn Trp Val Pro Leu Ile 
225                 230                 235                 240 


Arg Ala Gly Asp Trp Ser Val His Val Asp Arg Ile Thr Met Lys Gly 
                245                 250                 255     


Glu Val Ile Gly Cys Ser Asp Gly Cys Thr Ala Met Val Asp Thr Gly 
            260                 265                 270         


Ser Ser Asn Ile Gln Gly Pro Gly Arg Val Ile Asp Asn Ile His Lys 
        275                 280                 285             


Leu Ile Gly Ala Thr Pro Arg Gly Ser Lys His Tyr Val Ser Cys Ser 
    290                 295                 300                 


Ala Val Ser Ala Leu Pro Ser Val Val Phe Thr Ile Asn Gly Ile Asn 
305                 310                 315                 320 


Tyr Pro Val Pro Ala Arg Ala Tyr Val Leu Lys Asp Phe Thr Gly Asn 
                325                 330                 335     


Cys Tyr Thr Thr Phe Lys Glu Lys Arg Val Arg Arg Ser Thr Glu Phe 
            340                 345                 350         


Trp Ile Leu Gly Glu Ala Phe Leu Arg Leu Tyr Phe Ser Val Phe Asp 
        355                 360                 365             


Arg Gly Asn Asp Arg Ile Gly Leu Ala Arg Ala Val 
    370                 375                 380 


<210>  6
<211>  380
<212>  PRT
<213>  Bos taurus

<400>  6

Met Lys Trp Val Val Leu Leu Gly Leu Val Ala Phe Ser Glu Cys Ile 
1               5                   10                  15      


Val Lys Ile Pro Leu Arg Arg Val Lys Thr Met Arg Lys Thr Leu Ser 
            20                  25                  30          


Gly Lys Asn Met Leu Asn Asn Phe Leu Lys Glu His Gly Asn Arg Leu 
        35                  40                  45              


Ser Lys Ile Ser Phe Arg Gly Ser Asn Leu Thr Thr Leu Pro Leu Arg 
    50                  55                  60                  


Asn Ile Glu Asp Leu Met Tyr Val Gly Asn Ile Thr Ile Gly Thr Pro 
65                  70                  75                  80  


Pro Gln Glu Phe Gln Val Val Phe Asp Thr Gly Ser Ser Asp Phe Trp 
                85                  90                  95      


Val Pro Ser Asp Phe Cys Thr Ser Pro Asp Cys Ile Thr His Val Arg 
            100                 105                 110         


Phe Arg Gln His Gln Ser Ser Thr Phe Arg Pro Thr Asn Lys Thr Phe 
        115                 120                 125             


Ser Ile Thr Tyr Gly Ser Gly Arg Met Arg Gly Val Val Val His Asp 
    130                 135                 140                 


Thr Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln Pro Phe Gly Leu 
145                 150                 155                 160 


Ser Val Ser Glu Tyr Gly Phe Lys Asp Arg Ala Tyr Asp Gly Ile Leu 
                165                 170                 175     


Gly Leu Asn Tyr Pro Asp Glu Ser Phe Ser Glu Ala Ile Pro Ile Phe 
            180                 185                 190         


Asp Lys Leu Lys Asn Glu Gly Ala Ile Ser Glu Pro Ile Phe Ala Phe 
        195                 200                 205             


Tyr Leu Ser Lys Lys Lys Arg Glu Gly Ser Val Val Met Phe Gly Gly 
    210                 215                 220                 


Val Asp His Arg Tyr Tyr Lys Gly Glu Leu Asn Trp Val Pro Leu Ile 
225                 230                 235                 240 


Glu Glu Gly Asp Trp Ser Val Arg Met Asp Gly Ile Ser Met Lys Thr 
                245                 250                 255     


Lys Val Val Ala Cys Ser Asp Gly Cys Glu Ala Val Val Asp Thr Gly 
            260                 265                 270         


Thr Ser Leu Ile Lys Gly Pro Arg Lys Leu Val Asn Lys Ile Gln Lys 
        275                 280                 285             


Leu Ile Gly Ala Thr Pro Arg Gly Ser Lys His Tyr Val Tyr Cys Ser 
    290                 295                 300                 


Ala Val Asn Ala Leu Pro Ser Ile Ile Phe Thr Ile Asn Gly Ile Asn 
305                 310                 315                 320 


Tyr Pro Val Pro Ala Arg Ala Tyr Ile Leu Lys Asp Ser Arg Gly Arg 
                325                 330                 335     


Cys Tyr Thr Ala Phe Lys Lys Gln Arg Phe Ser Ser Ser Thr Glu Thr 
            340                 345                 350         


Trp Leu Leu Gly Asp Ala Phe Leu Arg Val Tyr Phe Ser Val Phe Asp 
        355                 360                 365             


Arg Gly Asn Gly Arg Ile Gly Leu Ala Gln Ala Val 
    370                 375                 380 


<210>  7
<211>  380
<212>  PRT
<213>  Bos taurus

<400>  7

Met Lys Trp Leu Val Leu Leu Gly Leu Val Ala Phe Ser Glu Cys Ile 
1               5                   10                  15      


Val Lys Ile Pro Leu Arg Arg Val Lys Thr Met Thr Lys Thr Leu Ser 
            20                  25                  30          


Gly Lys Asn Met Leu Asn Asn Phe Val Lys Glu His Ala Tyr Arg Leu 
        35                  40                  45              


Ser Gln Ile Ser Phe Arg Gly Ser Asn Leu Thr Ile His Pro Leu Arg 
    50                  55                  60                  


Asn Ile Arg Asp Phe Phe Tyr Val Gly Asn Ile Thr Ile Gly Thr Pro 
65                  70                  75                  80  


Pro Gln Glu Phe Gln Val Ile Phe Asp Thr Gly Ser Ser Glu Leu Trp 
                85                  90                  95      


Val Pro Ser Ile Phe Cys Asn Ser Ser Thr Cys Ser Lys His Asp Arg 
            100                 105                 110         


Phe Arg His Leu Glu Ser Ser Thr Phe Arg Leu Ser Arg Arg Thr Phe 
        115                 120                 125             


Ser Ile Thr Tyr Gly Ser Gly Arg Ile Glu Ala Leu Val Val His Asp 
    130                 135                 140                 


Thr Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln Gln Phe Gly Leu 
145                 150                 155                 160 


Cys Leu Glu Glu Ser Gly Phe Glu Gly Met Arg Phe Asp Gly Val Leu 
                165                 170                 175     


Gly Leu Ser Tyr Thr Asn Ile Ser Pro Ser Gly Ala Ile Pro Ile Phe 
            180                 185                 190         


Tyr Lys Leu Lys Asn Glu Gly Ala Ile Ser Glu Pro Val Phe Ala Phe 
        195                 200                 205             


Tyr Leu Ser Lys Asp Glu Arg Glu Gly Ser Val Val Met Phe Gly Gly 
    210                 215                 220                 


Ala Asp His Arg Tyr Tyr Lys Gly Glu Leu Asn Trp Ile Pro Leu Met 
225                 230                 235                 240 


Lys Ala Gly Asp Trp Ser Val His Met Asp Arg Ile Ser Met Lys Arg 
                245                 250                 255     


Lys Val Ile Ala Cys Ser Gly Gly Cys Lys Ala Leu Val Asp Thr Gly 
            260                 265                 270         


Ser Ser Asp Ile Val Gly Pro Ser Thr Leu Val Asn Asn Ile Trp Lys 
        275                 280                 285             


Leu Ile Gly Ala Thr Pro Gln Gly Ser Glu His Tyr Val Ser Cys Ser 
    290                 295                 300                 


Ala Val Asn Ser Leu Pro Ser Ile Ile Phe Thr Ile Lys Ser Asn Asn 
305                 310                 315                 320 


Tyr Arg Val Pro Gly Gln Ala Tyr Ile Leu Lys Asp Ser Arg Gly Arg 
                325                 330                 335     


Cys Phe Thr Ala Phe Lys Gly His Gln Gln Ser Ser Ser Thr Glu Met 
            340                 345                 350         


Trp Ile Leu Gly Asp Val Phe Leu Arg Leu Tyr Phe Ser Val Phe Asp 
        355                 360                 365             


Arg Arg Lys Asp Arg Ile Gly Leu Ala Thr Lys Val 
    370                 375                 380 


<210>  8
<211>  379
<212>  PRT
<213>  Bos taurus

<400>  8

Met Lys Trp Leu Val Leu Leu Gly Leu Val Ala Phe Ser Glu Cys Ile 
1               5                   10                  15      


Val Lys Ile Pro Leu Arg Arg Val Lys Thr Met Arg Asn Ala Ile Ser 
            20                  25                  30          


Gly Lys Asn Thr Leu Asn Asn Ile Leu Lys Glu His Ala Tyr Arg Leu 
        35                  40                  45              


Pro Gln Ile Ser Phe Arg Gly Ser Asn Leu Thr His Pro Leu Arg Asn 
    50                  55                  60                  


Ile Arg Asp Leu Phe Tyr Val Gly Asn Ile Thr Ile Gly Thr Pro Pro 
65                  70                  75                  80  


Gln Glu Phe Gln Val Ile Phe Asp Thr Gly Ser Ser Asp Leu Trp Val 
                85                  90                  95      


Ala Ser Ile Phe Cys Asn Ser Ser Ser Cys Ala Ala His Val Arg Phe 
            100                 105                 110         


Arg His His Gln Ser Ser Thr Phe Arg Pro Thr Asn Lys Thr Phe Arg 
        115                 120                 125             


Ile Thr Tyr Gly Ser Gly Arg Met Lys Gly Val Val Val His Asp Thr 
    130                 135                 140                 


Val Arg Ile Gly Asp Leu Val Ser Thr Asp Gln Pro Phe Gly Leu Cys 
145                 150                 155                 160 


Leu Lys Asp Ser Gly Phe Lys Gly Ile Pro Phe Asp Gly Ile Leu Gly 
                165                 170                 175     


Leu Ser Tyr Pro Asn Lys Thr Phe Ser Gly Ala Phe Pro Ile Phe Asp 
            180                 185                 190         


Lys Leu Lys Asn Glu Gly Ala Ile Ser Glu Pro Val Phe Ala Phe Tyr 
        195                 200                 205             


Leu Ser Lys Asp Lys Gln Glu Gly Ser Val Val Met Phe Gly Gly Val 
    210                 215                 220                 


Asp His Arg Tyr Tyr Lys Gly Glu Leu Asn Trp Val Pro Leu Ile Gln 
225                 230                 235                 240 


Val Gly Asp Trp Phe Val His Met Asp Arg Thr Thr Met Lys Arg Lys 
                245                 250                 255     


Val Ile Ala Cys Ser Asp Gly Cys Lys Ala Leu Val Asp Thr Gly Thr 
            260                 265                 270         


Ser Asp Ile Val Gly Pro Ser Thr Leu Val Asn Asn Ile Trp Lys Leu 
        275                 280                 285             


Ile Arg Ala Arg Pro Leu Gly Pro Gln Tyr Phe Val Ser Cys Ser Ala 
    290                 295                 300                 


Val Asn Thr Leu Pro Ser Ile Ile Phe Thr Ile Asn Gly Ile Asn Tyr 
305                 310                 315                 320 


Arg Leu Pro Ala Arg Ala Tyr Ile His Lys Asp Ser Arg Gly Arg Cys 
                325                 330                 335     


Tyr Thr Ala Phe Lys Glu His Arg Phe Ser Ser Pro Ile Glu Thr Trp 
            340                 345                 350         


Leu Leu Gly Asp Val Phe Leu Arg Arg Tyr Phe Ser Val Phe Asp Arg 
        355                 360                 365             


Gly Asn Asp Arg Ile Gly Leu Ala Arg Ala Val 
    370                 375                 


