 SEQUENCE LISTING

<110>  Glyco-therapy Biotechnology Co.,Ltd.

<120>  Site-specific Antibody Conjugates and the Methods for Preparation of the Same

<130>  0186-PA-004 

<160>  23

<170>  PatentIn version 3.5

<210>  1
<211>  336
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human 1,4-GalT1  (63-398; Y285L )

<400>  1

Gly Ser Asn Ser Ala Ala Ala Ile Gly Gln Ser Ser Gly Glu Leu Arg 
1               5                   10                  15      


Thr Gly Gly Ala Arg Pro Pro Pro Pro Leu Gly Ala Ser Ser Gln Pro 
            20                  25                  30          


Arg Pro Gly Gly Asp Ser Ser Pro Val Val Asp Ser Gly Pro Gly Pro 
        35                  40                  45              


Ala Ser Asn Leu Thr Ser Val Pro Val Pro His Thr Thr Ala Leu Ser 
    50                  55                  60                  


Leu Pro Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro Met Leu 
65                  70                  75                  80  


Ile Glu Phe Asn Met Pro Val Asp Leu Glu Leu Val Ala Lys Gln Asn 
                85                  90                  95      


Pro Asn Val Lys Met Gly Gly Arg Tyr Ala Pro Arg Asp Cys Val Ser 
            100                 105                 110         


Pro His Lys Val Ala Ile Ile Ile Pro Phe Arg Asn Arg Gln Glu His 
        115                 120                 125             


Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Val Leu Gln Arg Gln Gln 
    130                 135                 140                 


Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Asp Thr Ile Phe 
145                 150                 155                 160 


Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Gln Glu Ala Leu Lys Asp 
                165                 170                 175     


Tyr Asp Tyr Thr Cys Phe Val Phe Ser Asp Val Asp Leu Ile Pro Met 
            180                 185                 190         


Asn Asp His Asn Ala Tyr Arg Cys Phe Ser Gln Pro Arg His Ile Ser 
        195                 200                 205             


Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln Leu Phe 
    210                 215                 220                 


Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Thr Ile Asn Gly 
225                 230                 235                 240 


Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp Ile Phe 
                245                 250                 255     


Asn Arg Leu Val Phe Arg Gly Met Ser Ile Ser Arg Pro Asn Ala Val 
            260                 265                 270         


Val Gly Arg Cys Arg Met Ile Arg His Ser Arg Asp Lys Lys Asn Glu 
        275                 280                 285             


Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu Thr Met 
    290                 295                 300                 


Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Gln Val Leu Asp Val Gln 
305                 310                 315                 320 


Arg Tyr Pro Leu Tyr Thr Gln Ile Thr Val Asp Ile Gly Thr Pro Ser 
                325                 330                 335     


<210>  2
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine 1,4-GalT1(Y289L) (130-402;  Y289L; C342T ) with a N-terminal tag

<400>  2

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly Arg Gly Ser Ser Leu 
1               5                   10                  15      


Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro Met Leu Ile 
            20                  25                  30          


Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Val Glu Gln Gln Asn Pro 
        35                  40                  45              


Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys Ile Ser Pro 
    50                  55                  60                  


His Lys Val Ala Ile Ile Ile Pro Phe Arg Asn Arg Gln Glu His Leu 
65                  70                  75                  80  


Lys Tyr Trp Leu Tyr Tyr Leu His Pro Ile Leu Gln Arg Gln Gln Leu 
                85                  90                  95      


Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser Met Phe Asn 
            100                 105                 110         


Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu Lys Asp Tyr 
        115                 120                 125             


Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile Pro Met Asn 
    130                 135                 140                 


Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His Ile Ser Val 
145                 150                 155                 160 


Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln Leu Phe Gly 
                165                 170                 175     


Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile Asn Gly Phe 
            180                 185                 190         


Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp Ile Tyr Asn 
        195                 200                 205             


Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn Ala Val Ile 
    210                 215                 220                 


Gly Lys Thr Arg Met Ile Arg His Ser Arg Asp Lys Lys Asn Glu Pro 
225                 230                 235                 240 


Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu Thr Met Leu 
                245                 250                 255     


Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu Val Gln Arg 
            260                 265                 270         


Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr Pro Ser 
        275                 280                 285         


<210>  3
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Helicobacter pylori (1,3)-fucosyltransferase  (1-392)

<400>  3

Met Phe Gln Pro Leu Leu Asp Ala Phe Ile Glu Ser Ala Ser Ile Glu 
1               5                   10                  15      


Lys Met Val Ser Lys Ser Pro Pro Pro Pro Leu Lys Ile Ala Val Ala 
            20                  25                  30          


Asn Trp Trp Gly Asp Glu Glu Ile Lys Glu Phe Lys Lys Ser Val Leu 
        35                  40                  45              


Tyr Phe Ile Leu Ser Gln Arg Tyr Ala Ile Thr Leu His Gln Asn Pro 
    50                  55                  60                  


Asn Glu Ser Ser Asp Leu Val Phe Ser Asn Pro Leu Gly Ala Ala Arg 
65                  70                  75                  80  


Lys Ile Leu Ser Tyr Gln Asn Thr Lys Arg Val Phe Tyr Thr Gly Glu 
                85                  90                  95      


Asn Glu Ser Pro Asn Phe Asn Leu Phe Asp Tyr Ala Ile Gly Phe Asp 
            100                 105                 110         


Glu Leu Asp Phe Asn Asp Arg Tyr Leu Arg Met Pro Leu Tyr Tyr Ala 
        115                 120                 125             


His Leu His Tyr Glu Ala Glu Leu Val Asn Asp Thr Thr Ala Pro Tyr 
    130                 135                 140                 


Lys Leu Lys Asp Asn Ser Leu Tyr Ala Leu Lys Lys Pro Ser His His 
145                 150                 155                 160 


Phe Lys Glu Asn His Pro Asn Leu Cys Ala Val Val Asn Asp Glu Ser 
                165                 170                 175     


Asp Leu Leu Lys Arg Gly Phe Ala Ser Phe Val Ala Ser Asn Ala Asn 
            180                 185                 190         


Ala Pro Met Arg Asn Ala Phe Tyr Asp Ala Leu Asn Ser Ile Glu Pro 
        195                 200                 205             


Val Thr Gly Gly Gly Ser Val Arg Asn Thr Leu Gly Tyr Lys Val Gly 
    210                 215                 220                 


Asn Lys Ser Glu Phe Leu Ser Gln Tyr Lys Phe Asn Leu Cys Phe Glu 
225                 230                 235                 240 


Asn Ser Gln Gly Tyr Gly Tyr Val Thr Glu Lys Ile Leu Asp Ala Tyr 
                245                 250                 255     


Phe Ser His Thr Ile Pro Ile Tyr Trp Gly Ser Pro Ser Val Ala Lys 
            260                 265                 270         


Asp Phe Asn Pro Lys Ser Phe Val Asn Val His Asp Phe Asn Asn Phe 
        275                 280                 285             


Asp Glu Ala Ile Asp Tyr Ile Lys Tyr Leu His Thr His Pro Asn Ala 
    290                 295                 300                 


Tyr Leu Asp Met Leu Tyr Glu Asn Pro Leu Asn Thr Leu Asp Gly Lys 
305                 310                 315                 320 


Ala Tyr Phe Tyr Gln Asp Leu Ser Phe Lys Lys Ile Leu Asp Phe Phe 
                325                 330                 335     


Lys Thr Ile Leu Glu Asn Asp Thr Ile Tyr His Asn Asn Pro Phe Ile 
            340                 345                 350         


Phe Tyr Arg Asp Leu His Glu Pro Leu Ile Ser Ile Asp Asp Leu Arg 
        355                 360                 365             


Val Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg Val Asn 
    370                 375                 380                 


Tyr Asp Asp Leu Arg Val Asn Tyr 
385                 390         


<210>  4
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hp (1,3)-fucosyltransferase (1-392)  fusing with a C-terminal His tag

<400>  4

Met Phe Gln Pro Leu Leu Asp Ala Phe Ile Glu Ser Ala Ser Ile Glu 
1               5                   10                  15      


Lys Met Val Ser Lys Ser Pro Pro Pro Pro Leu Lys Ile Ala Val Ala 
            20                  25                  30          


Asn Trp Trp Gly Asp Glu Glu Ile Lys Glu Phe Lys Lys Ser Val Leu 
        35                  40                  45              


Tyr Phe Ile Leu Ser Gln Arg Tyr Ala Ile Thr Leu His Gln Asn Pro 
    50                  55                  60                  


Asn Glu Ser Ser Asp Leu Val Phe Ser Asn Pro Leu Gly Ala Ala Arg 
65                  70                  75                  80  


Lys Ile Leu Ser Tyr Gln Asn Thr Lys Arg Val Phe Tyr Thr Gly Glu 
                85                  90                  95      


Asn Glu Ser Pro Asn Phe Asn Leu Phe Asp Tyr Ala Ile Gly Phe Asp 
            100                 105                 110         


Glu Leu Asp Phe Asn Asp Arg Tyr Leu Arg Met Pro Leu Tyr Tyr Ala 
        115                 120                 125             


His Leu His Tyr Glu Ala Glu Leu Val Asn Asp Thr Thr Ala Pro Tyr 
    130                 135                 140                 


Lys Leu Lys Asp Asn Ser Leu Tyr Ala Leu Lys Lys Pro Ser His His 
145                 150                 155                 160 


Phe Lys Glu Asn His Pro Asn Leu Cys Ala Val Val Asn Asp Glu Ser 
                165                 170                 175     


Asp Leu Leu Lys Arg Gly Phe Ala Ser Phe Val Ala Ser Asn Ala Asn 
            180                 185                 190         


Ala Pro Met Arg Asn Ala Phe Tyr Asp Ala Leu Asn Ser Ile Glu Pro 
        195                 200                 205             


Val Thr Gly Gly Gly Ser Val Arg Asn Thr Leu Gly Tyr Lys Val Gly 
    210                 215                 220                 


Asn Lys Ser Glu Phe Leu Ser Gln Tyr Lys Phe Asn Leu Cys Phe Glu 
225                 230                 235                 240 


Asn Ser Gln Gly Tyr Gly Tyr Val Thr Glu Lys Ile Leu Asp Ala Tyr 
                245                 250                 255     


Phe Ser His Thr Ile Pro Ile Tyr Trp Gly Ser Pro Ser Val Ala Lys 
            260                 265                 270         


Asp Phe Asn Pro Lys Ser Phe Val Asn Val His Asp Phe Asn Asn Phe 
        275                 280                 285             


Asp Glu Ala Ile Asp Tyr Ile Lys Tyr Leu His Thr His Pro Asn Ala 
    290                 295                 300                 


Tyr Leu Asp Met Leu Tyr Glu Asn Pro Leu Asn Thr Leu Asp Gly Lys 
305                 310                 315                 320 


Ala Tyr Phe Tyr Gln Asp Leu Ser Phe Lys Lys Ile Leu Asp Phe Phe 
                325                 330                 335     


Lys Thr Ile Leu Glu Asn Asp Thr Ile Tyr His Asn Asn Pro Phe Ile 
            340                 345                 350         


Phe Tyr Arg Asp Leu His Glu Pro Leu Ile Ser Ile Asp Asp Leu Arg 
        355                 360                 365             


Val Asn Tyr Asp Asp Leu Arg Val Asn Tyr Asp Asp Leu Arg Val Asn 
    370                 375                 380                 


Tyr Asp Asp Leu Arg Val Asn Tyr Leu Glu His His His His His His 
385                 390                 395                 400 


<210>  5
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human FT6 catalytic domain (40-359) with a N terminal G

<400>  5

Gly Asp Pro Thr Val Tyr Pro Asn Gly Ser Arg Phe Pro Asp Ser Thr 
1               5                   10                  15      


Gly Thr Pro Ala His Ser Ile Pro Leu Ile Leu Leu Trp Thr Trp Pro 
            20                  25                  30          


Phe Asn Lys Pro Ile Ala Leu Pro Arg Cys Ser Glu Met Val Pro Gly 
        35                  40                  45              


Thr Ala Asp Cys Asn Ile Thr Ala Asp Arg Lys Val Tyr Pro Gln Ala 
    50                  55                  60                  


Asp Ala Val Ile Val His His Arg Glu Val Met Tyr Asn Pro Ser Ala 
65                  70                  75                  80  


Gln Leu Pro Arg Ser Ser Arg Arg Gln Gly Gln Arg Trp Ile Trp Phe 
                85                  90                  95      


Ser Met Glu Ser Pro Ser His Cys Trp Gln Leu Lys Ala Met Asp Gly 
            100                 105                 110         


Tyr Phe Asn Leu Thr Met Ser Tyr Arg Ser Asp Ser Asp Ile Phe Thr 
        115                 120                 125             


Pro Tyr Gly Trp Leu Glu Pro Trp Ser Gly Gln Pro Ala His Pro Pro 
    130                 135                 140                 


Leu Asn Leu Ser Ala Lys Thr Glu Leu Val Ala Trp Ala Val Ser Asn 
145                 150                 155                 160 


Trp Gly Pro Asn Ser Ala Arg Val Arg Tyr Tyr Gln Ser Leu Gln Ala 
                165                 170                 175     


His Leu Lys Val Asp Val Tyr Gly Arg Ser His Lys Pro Leu Pro Gln 
            180                 185                 190         


Gly Thr Met Met Glu Thr Leu Ser Arg Tyr Lys Phe Tyr Leu Ala Phe 
        195                 200                 205             


Lys Asn Ser Leu His Pro Asp Tyr Ile Thr Glu Lys Leu Trp Arg Asn 
    210                 215                 220                 


Ala Leu Glu Ala Trp Ala Val Pro Val Val Leu Gly Pro Ser Arg Ser 
225                 230                 235                 240 


Asn Tyr Glu Arg Phe Leu Pro Pro Asp Ala Phe Ile His Val Asp Asp 
                245                 250                 255     


Phe Gln Ser Pro Lys Asp Leu Ala Arg Tyr Leu Gln Glu Leu Asp Lys 
            260                 265                 270         


Asp His Ala Arg Tyr Leu Ser Tyr Phe Arg Trp Arg Glu Thr Leu Arg 
        275                 280                 285             


Pro Arg Ser Phe Ser Trp Ala Leu Ala Phe Cys Lys Ala Cys Trp Lys 
    290                 295                 300                 


Leu Gln Glu Glu Ser Arg Tyr Gln Thr Arg Gly Ile Ala Ala Trp Phe 
305                 310                 315                 320 


Thr 
    


<210>  6
<211>  1006
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptococcus pyogenes Endoglycosidase S  (37-995) fusing with a N-terminal tag

<400>  6

Met His His His His His His Ser Ser Gly Leu Val Pro Arg Gly Ser 
1               5                   10                  15      


Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp 
            20                  25                  30          


Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly Ile Glu Gly Arg Glu 
        35                  40                  45              


Glu Lys Thr Val Gln Val Gln Lys Gly Leu Pro Ser Ile Asp Ser Leu 
    50                  55                  60                  


His Tyr Leu Ser Glu Asn Ser Lys Lys Glu Phe Lys Glu Glu Leu Ser 
65                  70                  75                  80  


Lys Ala Gly Gln Glu Ser Gln Lys Val Lys Glu Ile Leu Ala Lys Ala 
                85                  90                  95      


Gln Gln Ala Asp Lys Gln Ala Gln Glu Leu Ala Lys Met Lys Ile Pro 
            100                 105                 110         


Glu Lys Ile Pro Met Lys Pro Leu His Gly Pro Leu Tyr Gly Gly Tyr 
        115                 120                 125             


Phe Arg Thr Trp His Asp Lys Thr Ser Asp Pro Thr Glu Lys Asp Lys 
    130                 135                 140                 


Val Asn Ser Met Gly Glu Leu Pro Lys Glu Val Asp Leu Ala Phe Ile 
145                 150                 155                 160 


Phe His Asp Trp Thr Lys Asp Tyr Ser Leu Phe Trp Lys Glu Leu Ala 
                165                 170                 175     


Thr Lys His Val Pro Lys Leu Asn Lys Gln Gly Thr Arg Val Ile Arg 
            180                 185                 190         


Thr Ile Pro Trp Arg Phe Leu Ala Gly Gly Asp Asn Ser Gly Ile Ala 
        195                 200                 205             


Glu Asp Thr Ser Lys Tyr Pro Asn Thr Pro Glu Gly Asn Lys Ala Leu 
    210                 215                 220                 


Ala Lys Ala Ile Val Asp Glu Tyr Val Tyr Lys Tyr Asn Leu Asp Gly 
225                 230                 235                 240 


Leu Asp Val Asp Val Glu His Asp Ser Ile Pro Lys Val Asp Lys Lys 
                245                 250                 255     


Glu Asp Thr Ala Gly Val Glu Arg Ser Ile Gln Val Phe Glu Glu Ile 
            260                 265                 270         


Gly Lys Leu Ile Gly Pro Lys Gly Val Asp Lys Ser Arg Leu Phe Ile 
        275                 280                 285             


Met Asp Ser Thr Tyr Met Ala Asp Lys Asn Pro Leu Ile Glu Arg Gly 
    290                 295                 300                 


Ala Pro Tyr Ile Asn Leu Leu Leu Val Gln Val Tyr Gly Ser Gln Gly 
305                 310                 315                 320 


Glu Lys Gly Gly Trp Glu Pro Val Ser Asn Arg Pro Glu Lys Thr Met 
                325                 330                 335     


Glu Glu Arg Trp Gln Gly Tyr Ser Lys Tyr Ile Arg Pro Glu Gln Tyr 
            340                 345                 350         


Met Ile Gly Phe Ser Phe Tyr Glu Glu Asn Ala Gln Glu Gly Asn Leu 
        355                 360                 365             


Trp Tyr Asp Ile Asn Ser Arg Lys Asp Glu Asp Lys Ala Asn Gly Ile 
    370                 375                 380                 


Asn Thr Asp Ile Thr Gly Thr Arg Ala Glu Arg Tyr Ala Arg Trp Gln 
385                 390                 395                 400 


Pro Lys Thr Gly Gly Val Lys Gly Gly Ile Phe Ser Tyr Ala Ile Asp 
                405                 410                 415     


Arg Asp Gly Val Ala His Gln Pro Lys Lys Tyr Ala Lys Gln Lys Glu 
            420                 425                 430         


Phe Lys Asp Ala Thr Asp Asn Ile Phe His Ser Asp Tyr Ser Val Ser 
        435                 440                 445             


Lys Ala Leu Lys Thr Val Met Leu Lys Asp Lys Ser Tyr Asp Leu Ile 
    450                 455                 460                 


Asp Glu Lys Asp Phe Pro Asp Lys Ala Leu Arg Glu Ala Val Met Ala 
465                 470                 475                 480 


Gln Val Gly Thr Arg Lys Gly Asp Leu Glu Arg Phe Asn Gly Thr Leu 
                485                 490                 495     


Arg Leu Asp Asn Pro Ala Ile Gln Ser Leu Glu Gly Leu Asn Lys Phe 
            500                 505                 510         


Lys Lys Leu Ala Gln Leu Asp Leu Ile Gly Leu Ser Arg Ile Thr Lys 
        515                 520                 525             


Leu Asp Arg Ser Val Leu Pro Ala Asn Met Lys Pro Gly Lys Asp Thr 
    530                 535                 540                 


Leu Glu Thr Val Leu Glu Thr Tyr Lys Lys Asp Asn Lys Glu Glu Pro 
545                 550                 555                 560 


Ala Thr Ile Pro Pro Val Ser Leu Lys Val Ser Gly Leu Thr Gly Leu 
                565                 570                 575     


Lys Glu Leu Asp Leu Ser Gly Phe Asp Arg Glu Thr Leu Ala Gly Leu 
            580                 585                 590         


Asp Ala Ala Thr Leu Thr Ser Leu Glu Lys Val Asp Ile Ser Gly Asn 
        595                 600                 605             


Lys Leu Asp Leu Ala Pro Gly Thr Glu Asn Arg Gln Ile Phe Asp Thr 
    610                 615                 620                 


Met Leu Ser Thr Ile Ser Asn His Val Gly Ser Asn Glu Gln Thr Val 
625                 630                 635                 640 


Lys Phe Asp Lys Gln Lys Pro Thr Gly His Tyr Pro Asp Thr Tyr Gly 
                645                 650                 655     


Lys Thr Ser Leu Arg Leu Pro Val Ala Asn Glu Lys Val Asp Leu Gln 
            660                 665                 670         


Ser Gln Leu Leu Phe Gly Thr Val Thr Asn Gln Gly Thr Leu Ile Asn 
        675                 680                 685             


Ser Glu Ala Asp Tyr Lys Ala Tyr Gln Asn His Lys Ile Ala Gly Arg 
    690                 695                 700                 


Ser Phe Val Asp Ser Asn Tyr His Tyr Asn Asn Phe Lys Val Ser Tyr 
705                 710                 715                 720 


Glu Asn Tyr Thr Val Lys Val Thr Asp Ser Thr Leu Gly Thr Thr Thr 
                725                 730                 735     


Asp Lys Thr Leu Ala Thr Asp Lys Glu Glu Thr Tyr Lys Val Asp Phe 
            740                 745                 750         


Phe Ser Pro Ala Asp Lys Thr Lys Ala Val His Thr Ala Lys Val Ile 
        755                 760                 765             


Val Gly Asp Glu Lys Thr Met Met Val Asn Leu Ala Glu Gly Ala Thr 
    770                 775                 780                 


Val Ile Gly Gly Ser Ala Asp Pro Val Asn Ala Arg Lys Val Phe Asp 
785                 790                 795                 800 


Gly Gln Leu Gly Ser Glu Thr Asp Asn Ile Ser Leu Gly Trp Asp Ser 
                805                 810                 815     


Lys Gln Ser Ile Ile Phe Lys Leu Lys Glu Asp Gly Leu Ile Lys His 
            820                 825                 830         


Trp Arg Phe Phe Asn Asp Ser Ala Arg Asn Pro Glu Thr Thr Asn Lys 
        835                 840                 845             


Pro Ile Gln Glu Ala Ser Leu Gln Ile Phe Asn Ile Lys Asp Tyr Asn 
    850                 855                 860                 


Leu Asp Asn Leu Leu Glu Asn Pro Asn Lys Phe Asp Asp Glu Lys Tyr 
865                 870                 875                 880 


Trp Ile Thr Val Asp Thr Tyr Ser Ala Gln Gly Glu Arg Ala Thr Ala 
                885                 890                 895     


Phe Ser Asn Thr Leu Asn Asn Ile Thr Ser Lys Tyr Trp Arg Val Val 
            900                 905                 910         


Phe Asp Thr Lys Gly Asp Arg Tyr Ser Ser Pro Val Val Pro Glu Leu 
        915                 920                 925             


Gln Ile Leu Gly Tyr Pro Leu Pro Asn Ala Asp Thr Ile Met Lys Thr 
    930                 935                 940                 


Val Thr Thr Ala Lys Glu Leu Ser Gln Gln Lys Asp Lys Phe Ser Gln 
945                 950                 955                 960 


Lys Met Leu Asp Glu Leu Lys Ile Lys Glu Met Ala Leu Glu Thr Ser 
                965                 970                 975     


Leu Asn Ser Lys Ile Phe Asp Val Thr Ala Ile Asn Ala Asn Ala Gly 
            980                 985                 990         


Val Leu Lys Asp Cys Ile Glu Lys Arg Gln Leu Leu Lys Lys 
        995                 1000                1005    


<210>  7
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lactobacillus casei 1,6-fucosidase (1-344) fusing with a CPD tag and a his tag in the C-terminus

<400>  7

Met Asn Asp Asn Val Ala Trp Phe Lys Gln Ala Lys Tyr Gly Met Met 
1               5                   10                  15      


Ile His Trp Gly Leu Tyr Ser Leu Leu Ala Gly Glu Tyr Arg Gly Glu 
            20                  25                  30          


Ser Ser Ser Ala Tyr Ala Glu Trp Ile Gln Ser Lys Phe Gln Ile Pro 
        35                  40                  45              


Asn Ala Glu Tyr Gly Asn Leu Ala Thr Ala Phe Asn Pro Leu Tyr Phe 
    50                  55                  60                  


Asp Ala Lys Lys Ile Val Ala Leu Ala Lys Gln Cys Gly Met Gln Tyr 
65                  70                  75                  80  


Leu Val Val Thr Thr Lys His His Asp Gly Phe Ala Met Tyr His Ser 
                85                  90                  95      


Lys Val Asp Ala Tyr Asn Val Tyr Asp Ala Thr Pro Phe His Arg Asp 
            100                 105                 110         


Ile Ile Gly Glu Leu Ala Glu Ala Cys Gln Lys Ala Gly Leu Lys Phe 
        115                 120                 125             


Gly Leu Tyr Tyr Ser Gln Asp Leu Asp Trp His Asp Pro Asn Gly Gly 
    130                 135                 140                 


Gly Tyr Lys Ser Asn Asp Val Glu Thr Ala Gly Thr Thr Trp Asp Asn 
145                 150                 155                 160 


Ser Trp Asp Phe Pro Asp Glu Asp Gln Lys Asn Phe Asp Leu Cys Phe 
                165                 170                 175     


Asp Asn Lys Ile Leu Pro Gln Ile Lys Glu Ile Met Ser Asn Tyr Gly 
            180                 185                 190         


Asp Ile Ala Thr Ala Trp Phe Asp Val Pro Met Thr Leu Ser Glu Ala 
        195                 200                 205             


Gln Ser Gln Thr Ile Tyr Asp Thr Val Arg Glu Leu Gln Pro Asn Cys 
    210                 215                 220                 


Leu Ile Asn Ser Arg Leu Gly Asn Gly Lys Tyr Asp Phe Val Ser Leu 
225                 230                 235                 240 


Gly Asp Asn Glu Ile Pro Lys Asn Lys Glu Asp Met Asn Lys Thr Asp 
                245                 250                 255     


Val Asp Tyr Asn Glu Ile Thr Gly Phe Lys Pro Ser Pro Leu Gly Leu 
            260                 265                 270         


Tyr Glu Thr Ala Gly Thr Ile Asn Asp Ser Trp Gly Phe Ser Tyr His 
        275                 280                 285             


Asp Gln Asn Trp Lys Thr Pro Arg Thr Leu Tyr Arg Tyr Lys Gln His 
    290                 295                 300                 


Leu Asn Asp Phe Gly Ile Asn Tyr Leu Leu Asn Val Gly Leu Asp Pro 
305                 310                 315                 320 


Leu Gly Arg Val Pro Met Met Ala Glu Glu Asn Leu Leu Ala Ala Lys 
                325                 330                 335     


Ala Leu Glu Asp Glu Ala Asn Arg Ala Leu Ala Asp Gly Lys Ile Leu 
            340                 345                 350         


His Asn Gln Asn Val Asn Ser Trp Gly Pro Ile Thr Val Thr Pro Thr 
        355                 360                 365             


Thr Asp Gly Gly Glu Thr Arg Phe Asp Gly Gln Ile Ile Val Gln Met 
    370                 375                 380                 


Glu Asn Asp Pro Val Val Ala Lys Ala Ala Ala Asn Leu Ala Gly Lys 
385                 390                 395                 400 


His Ala Glu Ser Ser Val Val Val Gln Leu Asp Ser Asp Gly Asn Tyr 
                405                 410                 415     


Arg Val Val Tyr Gly Asp Pro Ser Lys Leu Asp Gly Lys Leu Arg Trp 
            420                 425                 430         


Gln Leu Val Gly His Gly Arg Asp His Ser Glu Thr Asn Asn Thr Arg 
        435                 440                 445             


Leu Ser Gly Tyr Ser Ala Asp Glu Leu Ala Val Lys Leu Ala Lys Phe 
    450                 455                 460                 


Gln Gln Ser Phe Asn Gln Ala Glu Asn Ile Asn Asn Lys Pro Asp His 
465                 470                 475                 480 


Ile Ser Ile Val Gly Cys Ser Leu Val Ser Asp Asp Lys Gln Lys Gly 
                485                 490                 495     


Phe Gly His Gln Phe Ile Asn Ala Met Asp Ala Asn Gly Leu Arg Val 
            500                 505                 510         


Asp Val Ser Val Arg Ser Ser Glu Leu Ala Val Asp Glu Ala Gly Arg 
        515                 520                 525             


Lys His Thr Lys Asp Ala Asn Gly Asp Trp Val Gln Lys Ala Glu Asn 
    530                 535                 540                 


Asn Lys Val Ser Leu Ser Trp Asp Ala Gln Gly His His His His His 
545                 550                 555                 560 


His 
    


<210>  8
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  trastuzumab LC

<400>  8

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  9
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  trastuzumab HC

<400>  9

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  10
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rituximab LC

<400>  10

Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Ile 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Thr Ser Asn Pro Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  11
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rituximab HC

<400>  11

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ala Ser Ala Ser Thr Lys Gly Pro Ser 
        115                 120                 125             


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
    130                 135                 140                 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
            180                 185                 190         


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
        195                 200                 205             


Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Ala Glu Pro Lys Ser Cys 
    210                 215                 220                 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
            260                 265                 270         


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Pro Gly Lys 
    450     


<210>  12
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bevacizumab LC

<400>  12

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  13
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bevacizumab HC

<400>  13

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 
    50                  55                  60                  


Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
        115                 120                 125             


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
    130                 135                 140                 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
145                 150                 155                 160 


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
                165                 170                 175     


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
            180                 185                 190         


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
        195                 200                 205             


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
    210                 215                 220                 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
225                 230                 235                 240 


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                245                 250                 255     


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            260                 265                 270         


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
        275                 280                 285             


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
    290                 295                 300                 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
305                 310                 315                 320 


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
                325                 330                 335     


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            340                 345                 350         


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
        355                 360                 365             


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    370                 375                 380                 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
385                 390                 395                 400 


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
                405                 410                 415     


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
            420                 425                 430         


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
        435                 440                 445             


Leu Ser Pro Gly Lys 
    450             


<210>  14
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hRS7 LC

<400>  14

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  15
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hRS7 HC

<400>  15

Val Gln Leu Gln Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr Gly 
            20                  25                  30          


Met Asn Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Lys Trp Met Gly 
        35                  40                  45              


Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Thr Asp Asp Phe Lys 
    50                  55                  60                  


Gly Arg Phe Ala Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr Leu 
65                  70                  75                  80  


Gln Ile Ser Ser Leu Lys Ala Asp Asp Thr Ala Val Tyr Phe Cys Ala 
                85                  90                  95      


Arg Gly Gly Phe Gly Ser Ser Tyr Trp Tyr Phe Asp Val Trp Gly Gln 
            100                 105                 110         


Gly Ser Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  16
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bovine 1,4-GalT1(Y289L) (130-402;  Y289L; C342T ) without a N-terminal tag

<400>  16

Ser Leu Thr Ala Cys Pro Glu Glu Ser Pro Leu Leu Val Gly Pro Met 
1               5                   10                  15      


Leu Ile Glu Phe Asn Ile Pro Val Asp Leu Lys Leu Val Glu Gln Gln 
            20                  25                  30          


Asn Pro Lys Val Lys Leu Gly Gly Arg Tyr Thr Pro Met Asp Cys Ile 
        35                  40                  45              


Ser Pro His Lys Val Ala Ile Ile Ile Pro Phe Arg Asn Arg Gln Glu 
    50                  55                  60                  


His Leu Lys Tyr Trp Leu Tyr Tyr Leu His Pro Ile Leu Gln Arg Gln 
65                  70                  75                  80  


Gln Leu Asp Tyr Gly Ile Tyr Val Ile Asn Gln Ala Gly Glu Ser Met 
                85                  90                  95      


Phe Asn Arg Ala Lys Leu Leu Asn Val Gly Phe Lys Glu Ala Leu Lys 
            100                 105                 110         


Asp Tyr Asp Tyr Asn Cys Phe Val Phe Ser Asp Val Asp Leu Ile Pro 
        115                 120                 125             


Met Asn Asp His Asn Thr Tyr Arg Cys Phe Ser Gln Pro Arg His Ile 
    130                 135                 140                 


Ser Val Ala Met Asp Lys Phe Gly Phe Ser Leu Pro Tyr Val Gln Leu 
145                 150                 155                 160 


Phe Gly Gly Val Ser Ala Leu Ser Lys Gln Gln Phe Leu Ser Ile Asn 
                165                 170                 175     


Gly Phe Pro Asn Asn Tyr Trp Gly Trp Gly Gly Glu Asp Asp Asp Ile 
            180                 185                 190         


Tyr Asn Arg Leu Ala Phe Arg Gly Met Ser Val Ser Arg Pro Asn Ala 
        195                 200                 205             


Val Ile Gly Lys Thr Arg Met Ile Arg His Ser Arg Asp Lys Lys Asn 
    210                 215                 220                 


Glu Pro Asn Pro Gln Arg Phe Asp Arg Ile Ala His Thr Lys Glu Thr 
225                 230                 235                 240 


Met Leu Ser Asp Gly Leu Asn Ser Leu Thr Tyr Met Val Leu Glu Val 
                245                 250                 255     


Gln Arg Tyr Pro Leu Tyr Thr Lys Ile Thr Val Asp Ile Gly Thr Pro 
            260                 265                 270         


Ser 
    


<210>  17
<211>  959
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Streptococcus pyogenes Endoglycosidase S  (37-995)

<400>  17

Glu Glu Lys Thr Val Gln Val Gln Lys Gly Leu Pro Ser Ile Asp Ser 
1               5                   10                  15      


Leu His Tyr Leu Ser Glu Asn Ser Lys Lys Glu Phe Lys Glu Glu Leu 
            20                  25                  30          


Ser Lys Ala Gly Gln Glu Ser Gln Lys Val Lys Glu Ile Leu Ala Lys 
        35                  40                  45              


Ala Gln Gln Ala Asp Lys Gln Ala Gln Glu Leu Ala Lys Met Lys Ile 
    50                  55                  60                  


Pro Glu Lys Ile Pro Met Lys Pro Leu His Gly Pro Leu Tyr Gly Gly 
65                  70                  75                  80  


Tyr Phe Arg Thr Trp His Asp Lys Thr Ser Asp Pro Thr Glu Lys Asp 
                85                  90                  95      


Lys Val Asn Ser Met Gly Glu Leu Pro Lys Glu Val Asp Leu Ala Phe 
            100                 105                 110         


Ile Phe His Asp Trp Thr Lys Asp Tyr Ser Leu Phe Trp Lys Glu Leu 
        115                 120                 125             


Ala Thr Lys His Val Pro Lys Leu Asn Lys Gln Gly Thr Arg Val Ile 
    130                 135                 140                 


Arg Thr Ile Pro Trp Arg Phe Leu Ala Gly Gly Asp Asn Ser Gly Ile 
145                 150                 155                 160 


Ala Glu Asp Thr Ser Lys Tyr Pro Asn Thr Pro Glu Gly Asn Lys Ala 
                165                 170                 175     


Leu Ala Lys Ala Ile Val Asp Glu Tyr Val Tyr Lys Tyr Asn Leu Asp 
            180                 185                 190         


Gly Leu Asp Val Asp Val Glu His Asp Ser Ile Pro Lys Val Asp Lys 
        195                 200                 205             


Lys Glu Asp Thr Ala Gly Val Glu Arg Ser Ile Gln Val Phe Glu Glu 
    210                 215                 220                 


Ile Gly Lys Leu Ile Gly Pro Lys Gly Val Asp Lys Ser Arg Leu Phe 
225                 230                 235                 240 


Ile Met Asp Ser Thr Tyr Met Ala Asp Lys Asn Pro Leu Ile Glu Arg 
                245                 250                 255     


Gly Ala Pro Tyr Ile Asn Leu Leu Leu Val Gln Val Tyr Gly Ser Gln 
            260                 265                 270         


Gly Glu Lys Gly Gly Trp Glu Pro Val Ser Asn Arg Pro Glu Lys Thr 
        275                 280                 285             


Met Glu Glu Arg Trp Gln Gly Tyr Ser Lys Tyr Ile Arg Pro Glu Gln 
    290                 295                 300                 


Tyr Met Ile Gly Phe Ser Phe Tyr Glu Glu Asn Ala Gln Glu Gly Asn 
305                 310                 315                 320 


Leu Trp Tyr Asp Ile Asn Ser Arg Lys Asp Glu Asp Lys Ala Asn Gly 
                325                 330                 335     


Ile Asn Thr Asp Ile Thr Gly Thr Arg Ala Glu Arg Tyr Ala Arg Trp 
            340                 345                 350         


Gln Pro Lys Thr Gly Gly Val Lys Gly Gly Ile Phe Ser Tyr Ala Ile 
        355                 360                 365             


Asp Arg Asp Gly Val Ala His Gln Pro Lys Lys Tyr Ala Lys Gln Lys 
    370                 375                 380                 


Glu Phe Lys Asp Ala Thr Asp Asn Ile Phe His Ser Asp Tyr Ser Val 
385                 390                 395                 400 


Ser Lys Ala Leu Lys Thr Val Met Leu Lys Asp Lys Ser Tyr Asp Leu 
                405                 410                 415     


Ile Asp Glu Lys Asp Phe Pro Asp Lys Ala Leu Arg Glu Ala Val Met 
            420                 425                 430         


Ala Gln Val Gly Thr Arg Lys Gly Asp Leu Glu Arg Phe Asn Gly Thr 
        435                 440                 445             


Leu Arg Leu Asp Asn Pro Ala Ile Gln Ser Leu Glu Gly Leu Asn Lys 
    450                 455                 460                 


Phe Lys Lys Leu Ala Gln Leu Asp Leu Ile Gly Leu Ser Arg Ile Thr 
465                 470                 475                 480 


Lys Leu Asp Arg Ser Val Leu Pro Ala Asn Met Lys Pro Gly Lys Asp 
                485                 490                 495     


Thr Leu Glu Thr Val Leu Glu Thr Tyr Lys Lys Asp Asn Lys Glu Glu 
            500                 505                 510         


Pro Ala Thr Ile Pro Pro Val Ser Leu Lys Val Ser Gly Leu Thr Gly 
        515                 520                 525             


Leu Lys Glu Leu Asp Leu Ser Gly Phe Asp Arg Glu Thr Leu Ala Gly 
    530                 535                 540                 


Leu Asp Ala Ala Thr Leu Thr Ser Leu Glu Lys Val Asp Ile Ser Gly 
545                 550                 555                 560 


Asn Lys Leu Asp Leu Ala Pro Gly Thr Glu Asn Arg Gln Ile Phe Asp 
                565                 570                 575     


Thr Met Leu Ser Thr Ile Ser Asn His Val Gly Ser Asn Glu Gln Thr 
            580                 585                 590         


Val Lys Phe Asp Lys Gln Lys Pro Thr Gly His Tyr Pro Asp Thr Tyr 
        595                 600                 605             


Gly Lys Thr Ser Leu Arg Leu Pro Val Ala Asn Glu Lys Val Asp Leu 
    610                 615                 620                 


Gln Ser Gln Leu Leu Phe Gly Thr Val Thr Asn Gln Gly Thr Leu Ile 
625                 630                 635                 640 


Asn Ser Glu Ala Asp Tyr Lys Ala Tyr Gln Asn His Lys Ile Ala Gly 
                645                 650                 655     


Arg Ser Phe Val Asp Ser Asn Tyr His Tyr Asn Asn Phe Lys Val Ser 
            660                 665                 670         


Tyr Glu Asn Tyr Thr Val Lys Val Thr Asp Ser Thr Leu Gly Thr Thr 
        675                 680                 685             


Thr Asp Lys Thr Leu Ala Thr Asp Lys Glu Glu Thr Tyr Lys Val Asp 
    690                 695                 700                 


Phe Phe Ser Pro Ala Asp Lys Thr Lys Ala Val His Thr Ala Lys Val 
705                 710                 715                 720 


Ile Val Gly Asp Glu Lys Thr Met Met Val Asn Leu Ala Glu Gly Ala 
                725                 730                 735     


Thr Val Ile Gly Gly Ser Ala Asp Pro Val Asn Ala Arg Lys Val Phe 
            740                 745                 750         


Asp Gly Gln Leu Gly Ser Glu Thr Asp Asn Ile Ser Leu Gly Trp Asp 
        755                 760                 765             


Ser Lys Gln Ser Ile Ile Phe Lys Leu Lys Glu Asp Gly Leu Ile Lys 
    770                 775                 780                 


His Trp Arg Phe Phe Asn Asp Ser Ala Arg Asn Pro Glu Thr Thr Asn 
785                 790                 795                 800 


Lys Pro Ile Gln Glu Ala Ser Leu Gln Ile Phe Asn Ile Lys Asp Tyr 
                805                 810                 815     


Asn Leu Asp Asn Leu Leu Glu Asn Pro Asn Lys Phe Asp Asp Glu Lys 
            820                 825                 830         


Tyr Trp Ile Thr Val Asp Thr Tyr Ser Ala Gln Gly Glu Arg Ala Thr 
        835                 840                 845             


Ala Phe Ser Asn Thr Leu Asn Asn Ile Thr Ser Lys Tyr Trp Arg Val 
    850                 855                 860                 


Val Phe Asp Thr Lys Gly Asp Arg Tyr Ser Ser Pro Val Val Pro Glu 
865                 870                 875                 880 


Leu Gln Ile Leu Gly Tyr Pro Leu Pro Asn Ala Asp Thr Ile Met Lys 
                885                 890                 895     


Thr Val Thr Thr Ala Lys Glu Leu Ser Gln Gln Lys Asp Lys Phe Ser 
            900                 905                 910         


Gln Lys Met Leu Asp Glu Leu Lys Ile Lys Glu Met Ala Leu Glu Thr 
        915                 920                 925             


Ser Leu Asn Ser Lys Ile Phe Asp Val Thr Ala Ile Asn Ala Asn Ala 
    930                 935                 940                 


Gly Val Leu Lys Asp Cys Ile Glu Lys Arg Gln Leu Leu Lys Lys 
945                 950                 955                 


<210>  18
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lactobacillus casei 1,6-fucosidase (1-344)

<400>  18

Met Asn Asp Asn Val Ala Trp Phe Lys Gln Ala Lys Tyr Gly Met Met 
1               5                   10                  15      


Ile His Trp Gly Leu Tyr Ser Leu Leu Ala Gly Glu Tyr Arg Gly Glu 
            20                  25                  30          


Ser Ser Ser Ala Tyr Ala Glu Trp Ile Gln Ser Lys Phe Gln Ile Pro 
        35                  40                  45              


Asn Ala Glu Tyr Gly Asn Leu Ala Thr Ala Phe Asn Pro Leu Tyr Phe 
    50                  55                  60                  


Asp Ala Lys Lys Ile Val Ala Leu Ala Lys Gln Cys Gly Met Gln Tyr 
65                  70                  75                  80  


Leu Val Val Thr Thr Lys His His Asp Gly Phe Ala Met Tyr His Ser 
                85                  90                  95      


Lys Val Asp Ala Tyr Asn Val Tyr Asp Ala Thr Pro Phe His Arg Asp 
            100                 105                 110         


Ile Ile Gly Glu Leu Ala Glu Ala Cys Gln Lys Ala Gly Leu Lys Phe 
        115                 120                 125             


Gly Leu Tyr Tyr Ser Gln Asp Leu Asp Trp His Asp Pro Asn Gly Gly 
    130                 135                 140                 


Gly Tyr Lys Ser Asn Asp Val Glu Thr Ala Gly Thr Thr Trp Asp Asn 
145                 150                 155                 160 


Ser Trp Asp Phe Pro Asp Glu Asp Gln Lys Asn Phe Asp Leu Cys Phe 
                165                 170                 175     


Asp Asn Lys Ile Leu Pro Gln Ile Lys Glu Ile Met Ser Asn Tyr Gly 
            180                 185                 190         


Asp Ile Ala Thr Ala Trp Phe Asp Val Pro Met Thr Leu Ser Glu Ala 
        195                 200                 205             


Gln Ser Gln Thr Ile Tyr Asp Thr Val Arg Glu Leu Gln Pro Asn Cys 
    210                 215                 220                 


Leu Ile Asn Ser Arg Leu Gly Asn Gly Lys Tyr Asp Phe Val Ser Leu 
225                 230                 235                 240 


Gly Asp Asn Glu Ile Pro Lys Asn Lys Glu Asp Met Asn Lys Thr Asp 
                245                 250                 255     


Val Asp Tyr Asn Glu Ile Thr Gly Phe Lys Pro Ser Pro Leu Gly Leu 
            260                 265                 270         


Tyr Glu Thr Ala Gly Thr Ile Asn Asp Ser Trp Gly Phe Ser Tyr His 
        275                 280                 285             


Asp Gln Asn Trp Lys Thr Pro Arg Thr Leu Tyr Arg Tyr Lys Gln His 
    290                 295                 300                 


Leu Asn Asp Phe Gly Ile Asn Tyr Leu Leu Asn Val Gly Leu Asp Pro 
305                 310                 315                 320 


Leu Gly Arg Val Pro Met Met Ala Glu Glu Asn Leu Leu Ala Ala Lys 
                325                 330                 335     


Ala Leu Glu Asp Glu Ala Asn Arg 
            340                 


<210>  19
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  19
aaaaagcagg ctctgaaaac ttgtactttc aaggcggctc g                           41


<210>  20
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  20
ttgtacaaga aagctgggtc ctagctcggt gtcccgatgt c                           41


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo vector forward primer

<400>  21
gacccagctt tcttgtacaa agtg                                              24


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo vector reverse primer

<400>  22
gttttcagag cctgcttttt tgt                                               23


<210>  23
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-termianl LPETGG tag, LPETGG-COOH

<400>  23

Leu Pro Glu Thr Gly Gly 
1               5       

