                         SEQUENCE LISTING

<110>  SHANDONG BOAN BIOTECHNOLOGY CO., LTD.
 
<120>  Stealth Chimeric Antigen Receptor and Use Thereof in Reducing 
       Cytotoxicity towards Normal Cells

<130>  BA11

<150>  US 63/067,870
<151>  2020-08-19

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2R transmembrane domain (tm)

<400>  1

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2R degradation sequence (DT)

<400>  2

Pro Ser Lys Phe Phe Ser Gln Leu 
1               5               


<210>  3
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2R truncated cytoplasmic domain

<400>  3

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu 
            20                  25          


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL2R juxtamembrane sequence (jm)

<400>  4

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp 
            


<210>  5
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv

<400>  5

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe Asp Phe Thr Thr Tyr 
            20                  25                  30          


Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu 
    50                  55                  60                  


Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser Val Ala Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr 
            180                 185                 190         


Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Val Glu Ile Lys Gly 
                245     


<210>  6
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv-CD8Hinge-CD8tm-4-1BB-CD3 (LBC001)

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
305                 310                 315                 320 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
                325                 330                 335     


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            340                 345                 350         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        355                 360                 365             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490 


<210>  7
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-MN14op CEA scFv-CD8Hinge-CD8tm-4-1BB-CD3 (MLB001)

<400>  7

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Glu Val 
            20                  25                  30          


Gln Leu Val Glu Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser Leu 
        35                  40                  45              


Arg Leu Ser Cys Ser Ala Ser Gly Phe Asp Phe Thr Thr Tyr Trp Met 
    50                  55                  60                  


Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu 
65                  70                  75                  80  


Ile His Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu Lys Asp 
                85                  90                  95      


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln 
            100                 105                 110         


Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala Ser 
        115                 120                 125             


Leu Tyr Phe Gly Phe Pro Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
    130                 135                 140                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
                165                 170                 175     


Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Thr Cys 
            180                 185                 190         


Lys Ala Ser Gln Asp Val Gly Thr Ser Val Ala Trp Tyr Gln Gln Lys 
        195                 200                 205             


Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr Arg His 
    210                 215                 220                 


Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
225                 230                 235                 240 


Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr 
                245                 250                 255     


Cys Gln Gln Tyr Ser Leu Tyr Arg Ser Phe Gly Gly Gly Thr Lys Val 
            260                 265                 270         


Glu Ile Lys Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        275                 280                 285             


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    290                 295                 300                 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
305                 310                 315                 320 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
                325                 330                 335     


Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
            340                 345                 350         


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
        355                 360                 365             


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
    370                 375                 380                 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 


Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     


Pro Pro Arg 
            


<210>  8
<211>  507
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-MN14ops cFv-CD8Hinge-CD8 tm-4-1BB-CD3- DT (MLB002)

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Glu Val 
            20                  25                  30          


Gln Leu Val Glu Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser Leu 
        35                  40                  45              


Arg Leu Ser Cys Ser Ala Ser Gly Phe Asp Phe Thr Thr Tyr Trp Met 
    50                  55                  60                  


Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu 
65                  70                  75                  80  


Ile His Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu Lys Asp 
                85                  90                  95      


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln 
            100                 105                 110         


Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala Ser 
        115                 120                 125             


Leu Tyr Phe Gly Phe Pro Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
    130                 135                 140                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
                165                 170                 175     


Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Thr Cys 
            180                 185                 190         


Lys Ala Ser Gln Asp Val Gly Thr Ser Val Ala Trp Tyr Gln Gln Lys 
        195                 200                 205             


Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr Arg His 
    210                 215                 220                 


Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
225                 230                 235                 240 


Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr 
                245                 250                 255     


Cys Gln Gln Tyr Ser Leu Tyr Arg Ser Phe Gly Gly Gly Thr Lys Val 
            260                 265                 270         


Glu Ile Lys Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        275                 280                 285             


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    290                 295                 300                 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
305                 310                 315                 320 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
                325                 330                 335     


Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
            340                 345                 350         


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
        355                 360                 365             


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
    370                 375                 380                 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 


Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     


Pro Pro Arg Pro Ser Lys Phe Phe Ser Gln Leu 
            500                 505         


<210>  9
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-MN14op CEA scFv-CD8Hinge-IL2R tm jm DT-4-1BB-CD3 (MLB003)

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Glu Val 
            20                  25                  30          


Gln Leu Val Glu Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser Leu 
        35                  40                  45              


Arg Leu Ser Cys Ser Ala Ser Gly Phe Asp Phe Thr Thr Tyr Trp Met 
    50                  55                  60                  


Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu 
65                  70                  75                  80  


Ile His Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu Lys Asp 
                85                  90                  95      


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln 
            100                 105                 110         


Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala Ser 
        115                 120                 125             


Leu Tyr Phe Gly Phe Pro Trp Phe Ala Tyr Trp Gly Gln Gly Thr Thr 
    130                 135                 140                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
                165                 170                 175     


Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Thr Cys 
            180                 185                 190         


Lys Ala Ser Gln Asp Val Gly Thr Ser Val Ala Trp Tyr Gln Gln Lys 
        195                 200                 205             


Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr Arg His 
    210                 215                 220                 


Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
225                 230                 235                 240 


Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr 
                245                 250                 255     


Cys Gln Gln Tyr Ser Leu Tyr Arg Ser Phe Gly Gly Gly Thr Lys Val 
            260                 265                 270         


Glu Ile Lys Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        275                 280                 285             


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    290                 295                 300                 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
305                 310                 315                 320 


Asp Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe 
                325                 330                 335     


Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg Asn Thr Gly 
            340                 345                 350         


Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp Pro Ser Lys 
        355                 360                 365             


Phe Phe Ser Gln Leu Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    370                 375                 380                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
385                 390                 395                 400 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
                405                 410                 415     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
            420                 425                 430         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        435                 440                 445             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    450                 455                 460                 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
465                 470                 475                 480 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
                485                 490                 495     


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
            500                 505                 510         


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525         


<210>  10
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR-DTscFv

<400>  10

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
65                  70                  75                  80  


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                85                  90                  95      


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            100                 105                 110         


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
        115                 120                 125             


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    130                 135                 140                 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
145                 150                 155                 160 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                165                 170                 175     


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            180                 185                 190         


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        195                 200                 205             


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Pro 
    210                 215                 220                 


Ser Lys Phe Phe Ser Gln Leu 
225                 230     


<210>  11
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR- IL2R TM scFv

<400>  11

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Pro Trp 
        35                  40                  45              


Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile 
    50                  55                  60                  


Leu Val Tyr Leu Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys 
65                  70                  75                  80  


Lys Val Leu Lys Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln 
                85                  90                  95      


Leu Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            100                 105                 110         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        115                 120                 125             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    130                 135                 140                 


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
145                 150                 155                 160 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                165                 170                 175     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            180                 185                 190         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        195                 200                 205             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    210                 215                 220                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
225                 230                 235                 240 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250     


<210>  12
<211>  751
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv -CD8Hinge-CD8 tm-4-1BB-CD3-P2A-GFP (MLB010)

<400>  12

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
305                 310                 315                 320 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
                325                 330                 335     


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            340                 345                 350         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        355                 360                 365             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn 
                485                 490                 495     


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
            500                 505                 510         


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
        515                 520                 525             


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
    530                 535                 540                 


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
545                 550                 555                 560 


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
                565                 570                 575     


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
            580                 585                 590         


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
        595                 600                 605             


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
    610                 615                 620                 


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
625                 630                 635                 640 


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
                645                 650                 655     


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
            660                 665                 670         


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
        675                 680                 685             


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
    690                 695                 700                 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
705                 710                 715                 720 


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
                725                 730                 735     


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            740                 745                 750     


<210>  13
<211>  754
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin 18.2 scFv-CD8Hinge-CD8 tm-4-1BB-CD3-P2A-GFP 
       (MLB026)

<400>  13

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr 
        115                 120                 125             


Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser 
            180                 185                 190         


Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
                485                 490                 495     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            500                 505                 510         


Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
        515                 520                 525             


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
    530                 535                 540                 


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
545                 550                 555                 560 


Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
                565                 570                 575     


Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
            580                 585                 590         


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
        595                 600                 605             


Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
    610                 615                 620                 


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
625                 630                 635                 640 


Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
                645                 650                 655     


Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys 
            660                 665                 670         


Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
        675                 680                 685             


Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
    690                 695                 700                 


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
705                 710                 715                 720 


Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
                725                 730                 735     


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
            740                 745                 750         


Tyr Lys 
        


<210>  14
<211>  779
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv-CD8Hinge-IL2R tm jm DT-4-1BB-CD3-P2A-GFP 
       (MLB020)

<400>  14

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Pro Trp Leu Gly His Leu Leu 
305                 310                 315                 320 


Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu 
                325                 330                 335     


Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys 
            340                 345                 350         


Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Lys Arg Gly Arg 
        355                 360                 365             


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
    370                 375                 380                 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
385                 390                 395                 400 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                405                 410                 415     


Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            420                 425                 430         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        435                 440                 445             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
465                 470                 475                 480 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                485                 490                 495     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            500                 505                 510         


Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu 
        515                 520                 525             


Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys 
    530                 535                 540                 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
545                 550                 555                 560 


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
                565                 570                 575     


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
            580                 585                 590         


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
        595                 600                 605             


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
    610                 615                 620                 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
625                 630                 635                 640 


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
                645                 650                 655     


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
            660                 665                 670         


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
        675                 680                 685             


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
    690                 695                 700                 


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
705                 710                 715                 720 


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
                725                 730                 735     


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro 
            740                 745                 750         


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
        755                 760                 765             


Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775                 


<210>  15
<211>  786
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-MN14op CEA scFv-CD8Hinge-IL2R tm jm DT-4-1BB-CD3-P2A-GFP 
       (MLB013)

<400>  15

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Met Glu 
            20                  25                  30          


Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Lys Pro Gly Gly Ser 
        35                  40                  45              


Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe Asp Phe Thr Thr Tyr Trp 
    50                  55                  60                  


Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly 
65                  70                  75                  80  


Glu Ile His Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu Lys 
                85                  90                  95      


Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
            100                 105                 110         


Gln Met Asp Ser Leu Arg Pro Glu Asp Thr Gly Val Tyr Tyr Cys Ala 
        115                 120                 125             


Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
    130                 135                 140                 


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln 
                165                 170                 175     


Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Thr 
            180                 185                 190         


Cys Lys Ala Ser Gln Asp Val Gly Thr Ser Val Ala Trp Tyr Gln Gln 
        195                 200                 205             


Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Trp Thr Ser Thr Arg 
    210                 215                 220                 


His Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
225                 230                 235                 240 


Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro Glu Asp Ile Ala Thr Tyr 
                245                 250                 255     


Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser Phe Gly Gly Gly Thr Lys 
            260                 265                 270         


Val Glu Ile Lys Gly Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
        275                 280                 285             


Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
    290                 295                 300                 


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
305                 310                 315                 320 


Cys Asp Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala 
                325                 330                 335     


Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg Asn Thr 
            340                 345                 350         


Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp Pro Ser 
        355                 360                 365             


Lys Phe Phe Ser Gln Leu Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
    370                 375                 380                 


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
385                 390                 395                 400 


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
                405                 410                 415     


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
            420                 425                 430         


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
        435                 440                 445             


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
    450                 455                 460                 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
465                 470                 475                 480 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                485                 490                 495     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            500                 505                 510         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525             


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
    530                 535                 540                 


Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
545                 550                 555                 560 


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
                565                 570                 575     


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
            580                 585                 590         


Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
        595                 600                 605             


Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp 
    610                 615                 620                 


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
625                 630                 635                 640 


Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
                645                 650                 655     


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
            660                 665                 670         


Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
        675                 680                 685             


Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys 
    690                 695                 700                 


Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu 
705                 710                 715                 720 


Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
                725                 730                 735     


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln 
            740                 745                 750         


Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
        755                 760                 765             


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
    770                 775                 780                 


Tyr Lys 
785     


<210>  16
<211>  759
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv-CD8Hinge- CD8 tm-4-1BB-CD3-IL2R DT-P2A-GFP 
       (MLB025)

<400>  16

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala 
305                 310                 315                 320 


Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys 
                325                 330                 335     


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            340                 345                 350         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        355                 360                 365             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
    370                 375                 380                 


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
385                 390                 395                 400 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                405                 410                 415     


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            420                 425                 430         


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        435                 440                 445             


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    450                 455                 460                 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
465                 470                 475                 480 


Ala Leu His Met Gln Ala Leu Pro Pro Arg Pro Ser Lys Phe Phe Ser 
                485                 490                 495     


Gln Leu Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
            500                 505                 510         


Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu 
        515                 520                 525             


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
    530                 535                 540                 


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
545                 550                 555                 560 


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                565                 570                 575     


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
            580                 585                 590         


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
        595                 600                 605             


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
    610                 615                 620                 


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
625                 630                 635                 640 


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                645                 650                 655     


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
            660                 665                 670         


Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile 
        675                 680                 685             


Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    690                 695                 700                 


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
705                 710                 715                 720 


Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                725                 730                 735     


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            740                 745                 750         


Gly Met Asp Glu Leu Tyr Lys 
        755                 


<210>  17
<211>  781
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv-CD8Hinge-SEZ6L2 tm jm-4-1BB-CD3-P2A-GFP 
       (MLB047)

<400>  17

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Leu Ala Leu Ala Ile Leu Leu Pro 
305                 310                 315                 320 


Leu Gly Leu Val Ile Val Leu Gly Ser Gly Val Tyr Ile Tyr Tyr Thr 
                325                 330                 335     


Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly Ser His Ser Tyr 
            340                 345                 350         


Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro Leu Tyr Lys Arg 
        355                 360                 365             


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
    370                 375                 380                 


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
385                 390                 395                 400 


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
                405                 410                 415     


Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            420                 425                 430         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        435                 440                 445             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    450                 455                 460                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
465                 470                 475                 480 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                485                 490                 495     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            500                 505                 510         


His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn Phe Ser 
        515                 520                 525             


Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val 
    530                 535                 540                 


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
545                 550                 555                 560 


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
                565                 570                 575     


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
            580                 585                 590         


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr 
        595                 600                 605             


Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His 
    610                 615                 620                 


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
625                 630                 635                 640 


Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys 
                645                 650                 655     


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
            660                 665                 670         


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr 
        675                 680                 685             


Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile 
    690                 695                 700                 


Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln 
705                 710                 715                 720 


Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val 
                725                 730                 735     


Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys 
            740                 745                 750         


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
        755                 760                 765             


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775                 780     


<210>  18
<211>  767
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MN14op CEA scFv-CD8Hinge-LDLR tm jm-4-1BB-CD3-P2A-GFP (MLB048)

<400>  18

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val 
            20                  25                  30          


Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Phe 
        35                  40                  45              


Asp Phe Thr Thr Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile His Pro Asp Ser Ser Thr Ile Asn 
65                  70                  75                  80  


Tyr Ala Pro Ser Leu Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Pro Glu Asp Thr 
            100                 105                 110         


Gly Val Tyr Tyr Cys Ala Ser Leu Tyr Phe Gly Phe Pro Trp Phe Ala 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Asp Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
                165                 170                 175     


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ser 
            180                 185                 190         


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        195                 200                 205             


Tyr Trp Thr Ser Thr Arg His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    210                 215                 220                 


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
225                 230                 235                 240 


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Leu Tyr Arg Ser 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Thr Thr Thr Pro Ala 
            260                 265                 270         


Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser 
        275                 280                 285             


Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr 
    290                 295                 300                 


Arg Gly Leu Asp Phe Ala Cys Asp Ala Leu Ser Ile Val Leu Pro Ile 
305                 310                 315                 320 


Val Leu Leu Val Phe Leu Cys Leu Gly Val Phe Leu Leu Trp Lys Asn 
                325                 330                 335     


Trp Arg Leu Lys Asn Ile Asn Ser Ile Asn Phe Asp Asn Pro Val Tyr 
            340                 345                 350         


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
        355                 360                 365             


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
    370                 375                 380                 


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
385                 390                 395                 400 


Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn 
                405                 410                 415     


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            420                 425                 430         


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
        435                 440                 445             


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
    450                 455                 460                 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
465                 470                 475                 480 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
                485                 490                 495     


Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn 
            500                 505                 510         


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
        515                 520                 525             


Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
    530                 535                 540                 


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
545                 550                 555                 560 


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
                565                 570                 575     


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
            580                 585                 590         


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
        595                 600                 605             


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
    610                 615                 620                 


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
625                 630                 635                 640 


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
                645                 650                 655     


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
            660                 665                 670         


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
        675                 680                 685             


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
    690                 695                 700                 


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
705                 710                 715                 720 


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
                725                 730                 735     


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
            740                 745                 750         


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        755                 760                 765         


<210>  19
<211>  784
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin 18.2 scFv-CD8Hinge-SEZ6L2 tm jm-4-1BB-CD3-P2A-GFP 
       (MLB054)

<400>  19

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr 
        115                 120                 125             


Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser 
            180                 185                 190         


Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Leu Ala Leu Ala Ile 
305                 310                 315                 320 


Leu Leu Pro Leu Gly Leu Val Ile Val Leu Gly Ser Gly Val Tyr Ile 
                325                 330                 335     


Tyr Tyr Thr Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly Ser 
            340                 345                 350         


His Ser Tyr Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro Leu 
        355                 360                 365             


Tyr Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    370                 375                 380                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
385                 390                 395                 400 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
                405                 410                 415     


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
            420                 425                 430         


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        435                 440                 445             


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
    450                 455                 460                 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
465                 470                 475                 480 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
                485                 490                 495     


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
            500                 505                 510         


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr 
        515                 520                 525             


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 


Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
545                 550                 555                 560 


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
                565                 570                 575     


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
            580                 585                 590         


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
        595                 600                 605             


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
    610                 615                 620                 


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
625                 630                 635                 640 


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
                645                 650                 655     


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
            660                 665                 670         


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
        675                 680                 685             


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
    690                 695                 700                 


Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
705                 710                 715                 720 


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
                725                 730                 735     


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
            740                 745                 750         


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
        755                 760                 765             


Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775                 780                 


<210>  20
<211>  1169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin 18.2 scFv-CD8Hinge-SEZ6L2 tm jm-4-1BB-CD3-P2A- 
       PD-L1 scFv-CD8Hinge-CD8 tm-CD28-(G4S)2-GFP (MLB055)

<400>  20

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr 
        115                 120                 125             


Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser 
            180                 185                 190         


Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Leu Ala Leu Ala Ile 
305                 310                 315                 320 


Leu Leu Pro Leu Gly Leu Val Ile Val Leu Gly Ser Gly Val Tyr Ile 
                325                 330                 335     


Tyr Tyr Thr Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly Ser 
            340                 345                 350         


His Ser Tyr Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro Leu 
        355                 360                 365             


Tyr Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    370                 375                 380                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
385                 390                 395                 400 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
                405                 410                 415     


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
            420                 425                 430         


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        435                 440                 445             


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
    450                 455                 460                 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
465                 470                 475                 480 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
                485                 490                 495     


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
            500                 505                 510         


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr 
        515                 520                 525             


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 


Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu 
545                 550                 555                 560 


Leu His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Gly Gly 
                565                 570                 575     


Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
            580                 585                 590         


Phe Thr Phe Ser Asn Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
        595                 600                 605             


Lys Gly Leu Glu Trp Val Ala Ile Ile Thr Tyr Ala Gly Ser Asn Glu 
    610                 615                 620                 


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
625                 630                 635                 640 


Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp 
                645                 650                 655     


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Arg Ile Trp Val Asp Tyr Trp 
            660                 665                 670         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        675                 680                 685             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
    690                 695                 700                 


Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys 
705                 710                 715                 720 


Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu 
                725                 730                 735     


Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
            740                 745                 750         


Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        755                 760                 765             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu 
    770                 775                 780                 


Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro Leu Thr 
785                 790                 795                 800 


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Thr Thr Thr Pro Ala Pro 
                805                 810                 815     


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            820                 825                 830         


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        835                 840                 845             


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    850                 855                 860                 


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg 
865                 870                 875                 880 


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
                885                 890                 895     


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            900                 905                 910         


Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        915                 920                 925             


Gly Ser Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro 
    930                 935                 940                 


Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
945                 950                 955                 960 


Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys 
                965                 970                 975     


Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val 
            980                 985                 990         


Thr Thr Leu Thr Tyr Gly Val Gln  Cys Phe Ser Arg Tyr  Pro Asp His 
        995                 1000                 1005             


Met Lys  Gln His Asp Phe Phe  Lys Ser Ala Met Pro  Glu Gly Tyr 
    1010                 1015                 1020             


Val Gln  Glu Arg Thr Ile Phe  Phe Lys Asp Asp Gly  Asn Tyr Lys 
    1025                 1030                 1035             


Thr Arg  Ala Glu Val Lys Phe  Glu Gly Asp Thr Leu  Val Asn Arg 
    1040                 1045                 1050             


Ile Glu  Leu Lys Gly Ile Asp  Phe Lys Glu Asp Gly  Asn Ile Leu 
    1055                 1060                 1065             


Gly His  Lys Leu Glu Tyr Asn  Tyr Asn Ser His Asn  Val Tyr Ile 
    1070                 1075                 1080             


Met Ala  Asp Lys Gln Lys Asn  Gly Ile Lys Val Asn  Phe Lys Ile 
    1085                 1090                 1095             


Arg His  Asn Ile Glu Asp Gly  Ser Val Gln Leu Ala  Asp His Tyr 
    1100                 1105                 1110             


Gln Gln  Asn Thr Pro Ile Gly  Asp Gly Pro Val Leu  Leu Pro Asp 
    1115                 1120                 1125             


Asn His  Tyr Leu Ser Thr Gln  Ser Ala Leu Ser Lys  Asp Pro Asn 
    1130                 1135                 1140             


Glu Lys  Arg Asp His Met Val  Leu Leu Glu Phe Val  Thr Ala Ala 
    1145                 1150                 1155             


Gly Ile  Thr Leu Gly Met Asp  Glu Leu Tyr Lys 
    1160                 1165                 


<210>  21
<211>  777
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 scFv-CD8Hinge-IL2R tm jm DT-4-1BB-CD3-P2A-GFP (MLB038)

<400>  21

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
    50                  55                  60                  


Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His 
            100                 105                 110         


Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
145                 150                 155                 160 


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
                165                 170                 175     


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
            180                 185                 190         


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
        195                 200                 205             


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
    210                 215                 220                 


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro 
            260                 265                 270         


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
        275                 280                 285             


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
    290                 295                 300                 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Pro Trp Leu Gly His Leu 
305                 310                 315                 320 


Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu 
                325                 330                 335     


Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys 
            340                 345                 350         


Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Lys Arg Gly 
        355                 360                 365             


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
    370                 375                 380                 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
385                 390                 395                 400 


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
                405                 410                 415     


Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            420                 425                 430         


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        435                 440                 445             


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
    450                 455                 460                 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
465                 470                 475                 480 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
                485                 490                 495     


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
            500                 505                 510         


Met Gln Ala Leu Pro Pro Arg Ala Thr Asn Phe Ser Leu Leu Lys Gln 
        515                 520                 525             


Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu 
    530                 535                 540                 


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
545                 550                 555                 560 


Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
                565                 570                 575     


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
            580                 585                 590         


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln 
        595                 600                 605             


Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
    610                 615                 620                 


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
625                 630                 635                 640 


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
                645                 650                 655     


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
            660                 665                 670         


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
        675                 680                 685             


Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe 
    690                 695                 700                 


Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
705                 710                 715                 720 


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
                725                 730                 735     


Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu 
            740                 745                 750         


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 
        755                 760                 765             


Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775         


<210>  22
<211>  776
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 scFv-CD8Hinge-IL2R tm jm DT- CD28-CD3-P2A-GFP (MLB039)

<400>  22

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
    50                  55                  60                  


Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His 
            100                 105                 110         


Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
145                 150                 155                 160 


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
                165                 170                 175     


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
            180                 185                 190         


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
        195                 200                 205             


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
    210                 215                 220                 


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro 
            260                 265                 270         


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
        275                 280                 285             


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
    290                 295                 300                 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Pro Trp Leu Gly His Leu 
305                 310                 315                 320 


Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu 
                325                 330                 335     


Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys 
            340                 345                 350         


Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Arg Ser Lys 
        355                 360                 365             


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
    370                 375                 380                 


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
385                 390                 395                 400 


Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
                405                 410                 415     


Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
            420                 425                 430         


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
        435                 440                 445             


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
    450                 455                 460                 


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
465                 470                 475                 480 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
                485                 490                 495     


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
            500                 505                 510         


Gln Ala Leu Pro Pro Arg Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala 
        515                 520                 525             


Gly Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu 
    530                 535                 540                 


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
545                 550                 555                 560 


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
                565                 570                 575     


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
            580                 585                 590         


Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys 
        595                 600                 605             


Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
    610                 615                 620                 


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
625                 630                 635                 640 


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
                645                 650                 655     


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
            660                 665                 670         


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
        675                 680                 685             


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
    690                 695                 700                 


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
705                 710                 715                 720 


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
                725                 730                 735     


His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
            740                 745                 750         


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
        755                 760                 765             


Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775     


<210>  23
<211>  752
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 scFv-CD8Hinge-CD8 tm-4-1BB-CD3-P2A-GFP (MLB079)

<400>  23

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
    50                  55                  60                  


Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His 
            100                 105                 110         


Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
145                 150                 155                 160 


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
                165                 170                 175     


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
            180                 185                 190         


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
        195                 200                 205             


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
    210                 215                 220                 


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro 
            260                 265                 270         


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
        275                 280                 285             


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
    290                 295                 300                 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu 
305                 310                 315                 320 


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
                325                 330                 335     


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            340                 345                 350         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        355                 360                 365             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    370                 375                 380                 


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
385                 390                 395                 400 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                405                 410                 415     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            420                 425                 430         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        435                 440                 445             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    450                 455                 460                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
465                 470                 475                 480 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr 
                485                 490                 495     


Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
            500                 505                 510         


Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
        515                 520                 525             


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
    530                 535                 540                 


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
545                 550                 555                 560 


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
                565                 570                 575     


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
            580                 585                 590         


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
        595                 600                 605             


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
    610                 615                 620                 


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
625                 630                 635                 640 


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
                645                 650                 655     


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys 
            660                 665                 670         


Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
        675                 680                 685             


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
    690                 695                 700                 


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
705                 710                 715                 720 


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
                725                 730                 735     


Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            740                 745                 750         


<210>  24
<211>  782
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 scFv-CD8Hinge-SEZ6L2 tm jm-4-1BB-CD3-P2A-GFP (MLB080)

<400>  24

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala 
    50                  55                  60                  


Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                85                  90                  95      


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His 
            100                 105                 110         


Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
145                 150                 155                 160 


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
                165                 170                 175     


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
            180                 185                 190         


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
        195                 200                 205             


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
    210                 215                 220                 


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
225                 230                 235                 240 


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
                245                 250                 255     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr Thr Thr Pro 
            260                 265                 270         


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
        275                 280                 285             


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
    290                 295                 300                 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Leu Ala Leu Ala Ile Leu Leu 
305                 310                 315                 320 


Pro Leu Gly Leu Val Ile Val Leu Gly Ser Gly Val Tyr Ile Tyr Tyr 
                325                 330                 335     


Thr Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly Ser His Ser 
            340                 345                 350         


Tyr Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro Leu Tyr Lys 
        355                 360                 365             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    370                 375                 380                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
385                 390                 395                 400 


Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
                405                 410                 415     


Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            420                 425                 430         


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        435                 440                 445             


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    450                 455                 460                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
465                 470                 475                 480 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                485                 490                 495     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            500                 505                 510         


Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly Ala Thr Asn Phe 
        515                 520                 525             


Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
    530                 535                 540                 


Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
545                 550                 555                 560 


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
                565                 570                 575     


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
            580                 585                 590         


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
        595                 600                 605             


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
    610                 615                 620                 


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
625                 630                 635                 640 


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
                645                 650                 655     


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
            660                 665                 670         


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
        675                 680                 685             


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
    690                 695                 700                 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
705                 710                 715                 720 


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
                725                 730                 735     


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
            740                 745                 750         


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
        755                 760                 765             


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
    770                 775                 780         


<210>  25
<211>  1033
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin18.2 scFv-CD8Hinge-CD8 tm-4-1BB-CD3-P2A-HER2 
       scFv-IL2R tm jm DT-CD28-CD3 (MLB040)

<400>  25

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr 
        115                 120                 125             


Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser 
            180                 185                 190         


Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
                485                 490                 495     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            500                 505                 510         


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
        515                 520                 525             


Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro 
    530                 535                 540                 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
545                 550                 555                 560 


Ala Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro 
                565                 570                 575     


Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser 
            580                 585                 590         


Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr 
        595                 600                 605             


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
    610                 615                 620                 


Gln Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val 
625                 630                 635                 640 


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                645                 650                 655     


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            660                 665                 670         


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        675                 680                 685             


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    690                 695                 700                 


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
705                 710                 715                 720 


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                725                 730                 735     


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            740                 745                 750         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        755                 760                 765             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr 
    770                 775                 780                 


Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
785                 790                 795                 800 


Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
                805                 810                 815     


Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Pro Trp Leu 
            820                 825                 830         


Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu 
        835                 840                 845             


Val Tyr Leu Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys 
    850                 855                 860                 


Val Leu Lys Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu 
865                 870                 875                 880 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
                885                 890                 895     


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            900                 905                 910         


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        915                 920                 925             


Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    930                 935                 940                 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
945                 950                 955                 960 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                965                 970                 975     


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            980                 985                 990         


Ser Glu Ile Gly Met Lys Gly Glu  Arg Arg Arg Gly Lys  Gly His Asp 
        995                 1000                 1005             


Gly Leu  Tyr Gln Gly Leu Ser  Thr Ala Thr Lys Asp  Thr Tyr Asp 
    1010                 1015                 1020             


Ala Leu  His Met Gln Ala Leu  Pro Pro Arg 
    1025                 1030             


<210>  26
<211>  994
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin 18.2 scFv-CD8Hinge-CD8 tm-4-1BB-CD3-P2A-HER2 
       scFv-SEZ6L2 tm jm-CD3 (MLB108)

<400>  26

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly 
        35                  40                  45              


Thr Phe Ser Ser Tyr Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn 
65                  70                  75                  80  


Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser 
                85                  90                  95      


Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr 
        115                 120                 125             


Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
145                 150                 155                 160 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val 
                165                 170                 175     


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser 
            180                 185                 190         


Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
        195                 200                 205             


Ile Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
225                 230                 235                 240 


Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro 
                245                 250                 255     


Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
                485                 490                 495     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            500                 505                 510         


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
        515                 520                 525             


Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro 
    530                 535                 540                 


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
545                 550                 555                 560 


Ala Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro 
                565                 570                 575     


Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser 
            580                 585                 590         


Gly Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr 
        595                 600                 605             


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
    610                 615                 620                 


Gln Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val 
625                 630                 635                 640 


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                645                 650                 655     


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
            660                 665                 670         


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        675                 680                 685             


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    690                 695                 700                 


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
705                 710                 715                 720 


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                725                 730                 735     


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            740                 745                 750         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        755                 760                 765             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Thr 
    770                 775                 780                 


Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser 
785                 790                 795                 800 


Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly 
                805                 810                 815     


Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Leu Ala Leu Ala 
            820                 825                 830         


Ile Leu Leu Pro Leu Gly Leu Val Ile Val Leu Gly Ser Gly Val Tyr 
        835                 840                 845             


Ile Tyr Tyr Thr Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly 
    850                 855                 860                 


Ser His Ser Tyr Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro 
865                 870                 875                 880 


Leu Tyr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys 
                885                 890                 895     


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            900                 905                 910         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        915                 920                 925             


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    930                 935                 940                 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
945                 950                 955                 960 


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                965                 970                 975     


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            980                 985                 990         


Pro Arg 
        


<210>  27
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane linking juxtamembrane of IL2R

IL2R tm jm

<400>  27

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg Asn Thr Gly Pro 
            20                  25                  30          


Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp 
        35                  40                  


<210>  28
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane linking juxtamembrane of LDLR

LDLR tm jm

<400>  28

Ala Leu Ser Ile Val Leu Pro Ile Val Leu Leu Val Phe Leu Cys Leu 
1               5                   10                  15      


Gly Val Phe Leu Leu Trp Lys Asn Trp Arg Leu Lys Asn Ile Asn Ser 
            20                  25                  30          


Ile Asn Phe Asp Asn Pro Val Tyr 
        35                  40  


<210>  29
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane linking juxtamembrane of SEZ6LSEZ6L2 tm jm

<400>  29

Leu Ala Leu Ala Ile Leu Leu Pro Leu Gly Leu Val Ile Val Leu Gly 
1               5                   10                  15      


Ser Gly Val Tyr Ile Tyr Tyr Thr Lys Leu Gln Gly Lys Ser Leu Phe 
            20                  25                  30          


Gly Phe Ser Gly Ser His Ser Tyr Ser Pro Ile Thr Val Glu Ser Asp 
        35                  40                  45              


Phe Ser Asn Pro Leu Tyr 
    50                  


<210>  30
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  841 Claudin 18.2  scFv

<400>  30

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr Tyr Tyr Tyr Gly Met 
            100                 105                 110         


Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser Trp Leu Ala Trp Tyr 
                165                 170                 175     


Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser 
            180                 185                 190         


Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro Leu Thr Phe Gly Gly 
225                 230                 235                 240 


Gly Thr Lys Val Glu Ile Lys Arg Thr 
                245                 


<210>  31
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 scFv

<400>  31

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
        115                 120                 125             


Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
    130                 135                 140                 


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr 
145                 150                 155                 160 


Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala 
                165                 170                 175     


Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys 
            180                 185                 190         


Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu 
        195                 200                 205             


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser 
    210                 215                 220                 


Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
225                 230                 235                 240 


Thr Leu Val Thr Val Ser Ser 
                245         


<210>  32
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD-L1 scFv

<400>  32

Glu Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ile Ile Thr Tyr Ala Gly Ser Asn Glu Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Arg Ile Trp Val Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Asp Ser Leu Ala Val 
    130                 135                 140                 


Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val 
145                 150                 155                 160 


Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys 
                165                 170                 175     


Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
            180                 185                 190         


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
        195                 200                 205             


Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr 
    210                 215                 220                 


Cys Gln Gln Tyr Tyr Ser Thr Pro Leu Thr Phe Gly Gly Gly Thr Lys 
225                 230                 235                 240 


Val Glu Ile Lys 
                


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA

<400>  33

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  34
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 Hinge

<400>  34

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  35
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3

<400>  35

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  36
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB

<400>  36

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  37
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28

<400>  37

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  38
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane of LDLR LDLR tm 

<400>  38

Ala Leu Ser Ile Val Leu Pro Ile Val Leu Leu Val Phe Leu Cys Leu 
1               5                   10                  15      


Gly Val Phe Leu Leu Trp 
            20          


<210>  39
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  juxtamembrane of LDLRLDLR jm

<400>  39

Lys Asn Trp Arg Leu Lys Asn Ile Asn Ser Ile Asn Phe Asp Asn Pro 
1               5                   10                  15      


Val Tyr 
        


<210>  40
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane of SEZ6L2SEZ6L2 tm

<400>  40

Leu Ala Leu Ala Ile Leu Leu Pro Leu Gly Leu Val Ile Val Leu Gly 
1               5                   10                  15      


Ser Gly Val Tyr Ile 
            20      


<210>  41
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  juxtamembrane of SEZ6L2SEZ6L2 jm

<400>  41

Tyr Tyr Thr Lys Leu Gln Gly Lys Ser Leu Phe Gly Phe Ser Gly Ser 
1               5                   10                  15      


His Ser Tyr Ser Pro Ile Thr Val Glu Ser Asp Phe Ser Asn Pro Leu 
            20                  25                  30          


Tyr 
    


<210>  42
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane (CD8 tm)

<400>  42

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  43
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A-1

<400>  43

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  44
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A-2

<400>  44

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  45
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP

<400>  45

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  46
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leader Sequence

<400>  46

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  47
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of hCLDN18.2-808 antibody

<400>  47

Glu Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Leu Arg Phe Phe Asp Trp Leu Leu Gly Ser Ala Phe Asp 
            100                 105                 110         


Ile Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 


<210>  48
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of hCLDN18.2-808 antibody

<400>  48

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 
            20                  25                  30          


Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asp Tyr Asn Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  49
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of hCLDN18.2-841 antibody

<400>  49

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Ile Pro Ile Leu Gly Ile Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Val Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Arg Asp Asn Trp Asp Pro Tyr Tyr Tyr Tyr Tyr Gly Met 
            100                 105                 110         


Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


<210>  50
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of hCLDN18.2-841 antibody

<400>  50

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Phe Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


