                         SEQUENCE LISTING

<110>  Hadasit Medical Research Services and Development Ltd.
 
<120>  A DISINTEGRIN AND METALLOPROTEINASE WITH A THROMBOSPONDIN TYPE 1 
       MOTIF, MEMBER 13 (ADAMTS-13), MUTANTS, COMPOSITIONS AND 
       THERAPEUTIC METHODS THEREOF

<130>  2810995 SL/j

<150>  US 63/065,204
<151>  2020-08-13

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  4966
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  ADAMTS13 NM_139025.4

<400>  1
aaccacgatg tctttggcac agcctctcat ctgtcagatg ggagcgggga ccccggagag       60

ggagtcagcc gaggtcctgg cattccttgt gaacccccgt ctgtgggttt ctggtccagt      120

gtcccttctc cagattagat ggcttaggcc tcctctaagg gggtgggcgt gcacatccgg      180

agagctgtct ggtgtgcagg actgggctgc aggttaccct gaactgcaac catcttagag      240

caaggcccag cttgcagcag gaggagctgc aggccgccca ccctagccac ggcccctgcc      300

ctggcaggaa gcttccaaga gtaaacactg cctaatcgtc ccgcccagta gtgagcaggc      360

ctgtcccatt ccatactgac cagattccca gtcaccaagg ccccctctca ctccgctcca      420

ctcctcgggc tggctctcct gaggatgcac cagcgtcacc cccgggcaag atgccctccc      480

ctctgtgtgg ccggaatcct tgcctgtggc tttctcctgg gctgctgggg accctcccat      540

ttccagcaga gttgtcttca ggctttggag ccacaggccg tgtcttctta cttgagccct      600

ggtgctccct taaaaggccg ccctccttcc cctggcttcc agaggcagag gcagaggcag      660

aggcgggctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc      720

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      780

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      840

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      900

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      960

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg     1020

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag     1080

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg     1140

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac     1200

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc     1260

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc     1320

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgccgc     1380

gtggccttcg gccccaaggc tgtcgcctgc accttcgcca gggagcacct ggatatgtgc     1440

caggccctct cctgccacac agacccgctg gaccaaagca gctgcagccg cctcctcgtt     1500

cctctcctgg atgggacaga atgtggcgtg gagaagtggt gctccaaggg tcgctgccgc     1560

tccctggtgg agctgacccc catagcagca gtgcatgggc gctggtctag ctggggtccc     1620

cgaagtcctt gctcccgctc ctgcggagga ggtgtggtca ccaggaggcg gcagtgcaac     1680

aaccccagac ctgcctttgg ggggcgtgca tgtgttggtg ctgacctcca ggccgagatg     1740

tgcaacactc aggcctgcga gaagacccag ctggagttca tgtcgcaaca gtgcgccagg     1800

accgacggcc agccgctgcg ctcctcccct ggcggcgcct ccttctacca ctggggtgct     1860

gctgtaccac acagccaagg ggatgctctg tgcagacaca tgtgccgggc cattggcgag     1920

agcttcatca tgaagcgtgg agacagcttc ctcgatggga cccggtgtat gccaagtggc     1980

ccccgggagg acgggaccct gagcctgtgt gtgtcgggca gctgcaggac atttggctgt     2040

gatggtagga tggactccca gcaggtatgg gacaggtgcc aggtgtgtgg tggggacaac     2100

agcacgtgca gcccacggaa gggctctttc acagctggca gagcgagaga atatgtcacg     2160

tttctgacag ttacccccaa cctgaccagt gtctacattg ccaaccacag gcctctcttc     2220

acacacttgg cggtgaggat cggagggcgc tatgtcgtgg ctgggaagat gagcatctcc     2280

cctaacacca cctacccctc cctcctggag gatggtcgtg tcgagtacag agtggccctc     2340

accgaggacc ggctgccccg cctggaggag atccgcatct ggggacccct ccaggaagat     2400

gctgacatcc aggtttacag gcggtatggc gaggagtatg gcaacctcac ccgcccagac     2460

atcaccttca cctacttcca gcctaagcca cggcaggcct gggtgtgggc cgctgtgcgt     2520

gggccctgct cggtgagctg tggggcaggg ctgcgctggg taaactacag ctgcctggac     2580

caggccagga aggagttggt ggagactgtc cagtgccaag ggagccagca gccaccagcg     2640

tggccagagg cctgcgtgct cgaaccctgc cctccctact gggcggtggg agacttcggc     2700

ccatgcagcg cctcctgtgg gggtggcctg cgggagcggc cagtgcgctg cgtggaggcc     2760

cagggcagcc tcctgaagac attgccccca gcccggtgca gagcaggggc ccagcagcca     2820

gctgtggcgc tggaaacctg caacccccag ccctgccctg ccaggtggga ggtgtcagag     2880

cccagctcat gcacatcagc tggtggagca ggcctggcct tggagaacga gacctgtgtg     2940

ccaggggcag atggcctgga ggctccagtg actgaggggc ctggctccgt agatgagaag     3000

ctgcctgccc ctgagccctg tgtcgggatg tcatgtcctc caggctgggg ccatctggat     3060

gccacctctg caggggagaa ggctccctcc ccatggggca gcatcaggac gggggctcaa     3120

gctgcacacg tgtggacccc tgcggcaggg tcgtgctccg tctcctgcgg gcgaggtctg     3180

atggagctgc gtttcctgtg catggactct gccctcaggg tgcctgtcca ggaagagctg     3240

tgtggcctgg caagcaagcc tgggagccgg cgggaggtct gccaggctgt cccgtgccct     3300

gctcggtggc agtacaagct ggcggcctgc agcgtgagct gtgggagagg ggtcgtgcgg     3360

aggatcctgt attgtgcccg ggcccatggg gaggacgatg gtgaggagat cctgttggac     3420

acccagtgcc aggggctgcc tcgcccggaa ccccaggagg cctgcagcct ggagccctgc     3480

ccacctaggt ggaaagtcat gtcccttggc ccatgttcgg ccagctgtgg ccttggcact     3540

gctagacgct cggtggcctg tgtgcagctc gaccaaggcc aggacgtgga ggtggacgag     3600

gcggcctgtg cggcgctggt gcggcccgag gccagtgtcc cctgtctcat tgccgactgc     3660

acctaccgct ggcatgttgg cacctggatg gagtgctctg tttcctgtgg ggatggcatc     3720

cagcgccggc gtgacacctg cctcggaccc caggcccagg cgcctgtgcc agctgatttc     3780

tgccagcact tgcccaagcc ggtgactgtg cgtggctgct gggctgggcc ctgtgtggga     3840

cagggtacgc ccagcctggt gccccacgaa gaagccgctg ctccaggacg gaccacagcc     3900

acccctgctg gtgcctccct ggagtggtcc caggcccggg gcctgctctt ctccccggct     3960

ccccagcctc ggcggctcct gcccgggccc caggaaaact cagtgcagtc cagtgcctgt     4020

ggcaggcagc accttgagcc aacaggaacc attgacatgc gaggcccagg gcaggcagac     4080

tgtgcagtgg ccattgggcg gcccctcggg gaggtggtga ccctccgcgt ccttgagagt     4140

tctctcaact gcagtgcggg ggacatgttg ctgctttggg gccggctcac ctggaggaag     4200

atgtgcagga agctgttgga catgactttc agctccaaga ccaacacgct ggtggtgagg     4260

cagcgctgcg ggcggccagg aggtggggtg ctgctgcggt atgggagcca gcttgctcct     4320

gaaaccttct acagagaatg tgacatgcag ctctttgggc cctggggtga aatcgtgagc     4380

ccctcgctga gtccagccac gagtaatgca gggggctgcc ggctcttcat taatgtggct     4440

ccgcacgcac ggattgccat ccatgccctg gccaccaaca tgggcgctgg gaccgaggga     4500

gccaatgcca gctacatctt gatccgggac acccacagct tgaggaccac agcgttccat     4560

gggcagcagg tgctctactg ggagtcagag agcagccagg ctgagatgga gttcagcgag     4620

ggcttcctga aggctcaggc cagcctgcgg ggccagtact ggaccctcca atcatgggta     4680

ccggagatgc aggaccctca gtcctggaag ggaaaggaag gaacctgagg gtcattgaac     4740

atttgttccg tgtctggcca gccctggagg gttgacccct ggtctcagtg ctttccaatt     4800

cgaacttttt ccaatcttag gtatctactt tagagtcttc tccaatgtcc aaaaggctag     4860

ggggttggag gtggggactc tggaaaagca gcccccattt cctcgggtac caataaataa     4920

aacatgcagg ctgaccggcg tttttttctt aaaaaaaaaa aaaaaa                    4966


<210>  2
<211>  1427
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  ADAMTS NP_620594.1

<400>  2

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Arg Val Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  3
<211>  2954
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  tissue plasminogen activator, NM_001319189.1

<400>  3
gaacttcctc cccctgcttt ataaaaacag gcctgcctca gctccctcat ggccctgtcc       60

actgagcatc ctcccgccac acagaaaccc gcccagccgg ggccaccgac cccaccccct      120

gcctggaaac ttaaaggagg ccggagctgt ggggagctca gagctgagat cctacaggag      180

tccagggctg gagagaaaac ctctgcgagg aaagggaagg agcaagccgt gaatttaagg      240

gacgctgtga agcaatcatg gatgcaatga agagagggct ctgctgtgtg ctgctgctgt      300

gtggagcagt cttcgtttcg cccagccagg aaatccatgc ccgattcaga agaggagcca      360

gatcttacca agtgatctgc agagatgaaa aaacgcagat gatataccag caacatcagt      420

catggctgcg ccctgtgctc agaagcaacc gggtggaata ttgctggtgc aacagtggca      480

gggcacagtg ccactcagtg cctgtcaaaa gttgcagcga gccaaggtgt ttcaacgggg      540

gcacctgcca gcaggccctg tacttctcag atttcgtgtg ccagtgcccc gaaggatttg      600

ctgggaagtg ctgtgaaata ggaaacagtg actgctactt tgggaatggg tcagcctacc      660

gtggcacgca cagcctcacc gagtcgggtg cctcctgcct cccgtggaat tccatgatcc      720

tgataggcaa ggtttacaca gcacagaacc ccagtgccca ggcactgggc ctgggcaaac      780

ataattactg ccggaatcct gatggggatg ccaagccctg gtgccacgtg ctgaagaacc      840

gcaggctgac gtgggagtac tgtgatgtgc cctcctgctc cacctgcggc ctgagacagt      900

acagccagcc tcagtttcgc atcaaaggag ggctcttcgc cgacatcgcc tcccacccct      960

ggcaggctgc catctttgcc aagcacagga ggtcgcccgg agagcggttc ctgtgcgggg     1020

gcatactcat cagctcctgc tggattctct ctgccgccca ctgcttccag gagaggtttc     1080

cgccccacca cctgacggtg atcttgggca gaacataccg ggtggtccct ggcgaggagg     1140

agcagaaatt tgaagtcgaa aaatacattg tccataagga attcgatgat gacacttacg     1200

acaatgacat tgcgctgctg cagctgaaat cggattcgtc ccgctgtgcc caggagagca     1260

gcgtggtccg cactgtgtgc cttcccccgg cggacctgca gctgccggac tggacggagt     1320

gtgagctctc cggctacggc aagcatgagg ccttgtctcc tttctattcg gagcggctga     1380

aggaggctca tgtcagactg tacccatcca gccgctgcac atcacaacat ttacttaaca     1440

gaacagtcac cgacaacatg ctgtgtgctg gagacactcg gagcggcggg ccccaggcaa     1500

acttgcacga cgcctgccag ggcgattcgg gaggccccct ggtgtgtctg aacgatggcc     1560

gcatgacttt ggtgggcatc atcagctggg gcctgggctg tggacagaag gatgtcccgg     1620

gtgtgtacac caaggttacc aactacctag actggattcg tgacaacatg cgaccgtgac     1680

caggaacacc cgactcctca aaagcaaatg agatcccgcc tcttcttctt cagaagacac     1740

tgcaaaggcg cagtgcttct ctacagactt ctccagaccc accacaccgc agaagcggga     1800

cgagacccta caggagaggg aagagtgcat tttcccagat acttcccatt ttggaagttt     1860

tcaggacttg gtctgatttc aggatactct gtcagatggg aagacatgaa tgcacactag     1920

cctctccagg aatgcctcct ccctgggcag aaagtggcca tgccaccctg ttttcagcta     1980

aagcccaacc tcctgacctg tcaccgtgag cagctttgga aacaggacca caaaaatgaa     2040

agcatgtctc aatagtaaaa gataacaaga tctttcagga aagacggatt gcattagaaa     2100

tagacagtat atttatagtc acaagagccc agcagggcct caaagttggg gcaggctggc     2160

tggcccgtca tgttcctcaa aagcaccctt gacgtcaagt ctccttcccc tttccccact     2220

ccctggctct cagaaggtat tccttttgtg tacagtgtgt aaagtgtaaa tcctttttct     2280

ttataaactt tagagtagca tgagagaatt gtatcatttg aacaactagg cttcagcata     2340

tttatagcaa tccatgttag tttttacttt ctgttgccac aaccctgttt tatactgtac     2400

ttaataaatt cagatatatt tttcacagtt tttccaaaat cagagtggaa tggttttgtt     2460

atagatgctg tatcccactc tttattcatg ttcacatttt aaaatcattt ggaattctgc     2520

ttcactcgct taacatatac acaacacctg taacatacaa ggcaatgggc taggtgctcc     2580

agaccgggaa aaggagggac aggaatgctt ggtctgatgg gctaatatgg catttagaga     2640

agtaccaagg tacagtggag ccggtcacaa aagggcagac ttgtagtaga attcagttgc     2700

aagagggatt ggggaatctt aaggaaaaaa tagaatctta aggaaaaaat aactgggtga     2760

gacgtggact gtggacaggt gtggaaaagg cactctccat ggaggtatga atatgtagag     2820

ggccaagaga ggggagtaca gggagaaatg agttgagctt gtctgaagtg aacttcagga     2880

agaggaacat aggctggaat ttagattatg ggggctctga acaccaaact gagtttggac     2940

ttaattgact tctg                                                       2954


<210>  4
<211>  473
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  tissue plasminogen activator, NP_001306118.1

<400>  4

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Ser Gln Glu Ile His Ala Arg Phe Arg Arg 
            20                  25                  30          


Gly Ala Arg Ser Tyr Gln Val Ile Cys Arg Asp Glu Lys Thr Gln Met 
        35                  40                  45              


Ile Tyr Gln Gln His Gln Ser Trp Leu Arg Pro Val Leu Arg Ser Asn 
    50                  55                  60                  


Arg Val Glu Tyr Cys Trp Cys Asn Ser Gly Arg Ala Gln Cys His Ser 
65                  70                  75                  80  


Val Pro Val Lys Ser Cys Ser Glu Pro Arg Cys Phe Asn Gly Gly Thr 
                85                  90                  95      


Cys Gln Gln Ala Leu Tyr Phe Ser Asp Phe Val Cys Gln Cys Pro Glu 
            100                 105                 110         


Gly Phe Ala Gly Lys Cys Cys Glu Ile Gly Asn Ser Asp Cys Tyr Phe 
        115                 120                 125             


Gly Asn Gly Ser Ala Tyr Arg Gly Thr His Ser Leu Thr Glu Ser Gly 
    130                 135                 140                 


Ala Ser Cys Leu Pro Trp Asn Ser Met Ile Leu Ile Gly Lys Val Tyr 
145                 150                 155                 160 


Thr Ala Gln Asn Pro Ser Ala Gln Ala Leu Gly Leu Gly Lys His Asn 
                165                 170                 175     


Tyr Cys Arg Asn Pro Asp Gly Asp Ala Lys Pro Trp Cys His Val Leu 
            180                 185                 190         


Lys Asn Arg Arg Leu Thr Trp Glu Tyr Cys Asp Val Pro Ser Cys Ser 
        195                 200                 205             


Thr Cys Gly Leu Arg Gln Tyr Ser Gln Pro Gln Phe Arg Ile Lys Gly 
    210                 215                 220                 


Gly Leu Phe Ala Asp Ile Ala Ser His Pro Trp Gln Ala Ala Ile Phe 
225                 230                 235                 240 


Ala Lys His Arg Arg Ser Pro Gly Glu Arg Phe Leu Cys Gly Gly Ile 
                245                 250                 255     


Leu Ile Ser Ser Cys Trp Ile Leu Ser Ala Ala His Cys Phe Gln Glu 
            260                 265                 270         


Arg Phe Pro Pro His His Leu Thr Val Ile Leu Gly Arg Thr Tyr Arg 
        275                 280                 285             


Val Val Pro Gly Glu Glu Glu Gln Lys Phe Glu Val Glu Lys Tyr Ile 
    290                 295                 300                 


Val His Lys Glu Phe Asp Asp Asp Thr Tyr Asp Asn Asp Ile Ala Leu 
305                 310                 315                 320 


Leu Gln Leu Lys Ser Asp Ser Ser Arg Cys Ala Gln Glu Ser Ser Val 
                325                 330                 335     


Val Arg Thr Val Cys Leu Pro Pro Ala Asp Leu Gln Leu Pro Asp Trp 
            340                 345                 350         


Thr Glu Cys Glu Leu Ser Gly Tyr Gly Lys His Glu Ala Leu Ser Pro 
        355                 360                 365             


Phe Tyr Ser Glu Arg Leu Lys Glu Ala His Val Arg Leu Tyr Pro Ser 
    370                 375                 380                 


Ser Arg Cys Thr Ser Gln His Leu Leu Asn Arg Thr Val Thr Asp Asn 
385                 390                 395                 400 


Met Leu Cys Ala Gly Asp Thr Arg Ser Gly Gly Pro Gln Ala Asn Leu 
                405                 410                 415     


His Asp Ala Cys Gln Gly Asp Ser Gly Gly Pro Leu Val Cys Leu Asn 
            420                 425                 430         


Asp Gly Arg Met Thr Leu Val Gly Ile Ile Ser Trp Gly Leu Gly Cys 
        435                 440                 445             


Gly Gln Lys Asp Val Pro Gly Val Tyr Thr Lys Val Thr Asn Tyr Leu 
    450                 455                 460                 


Asp Trp Ile Arg Asp Asn Met Arg Pro 
465                 470             


<210>  5
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated R312K truncated

<400>  5

Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val Gly Pro 
1               5                   10                  15      


Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr 
            20                  25                  30          


Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala 
        35                  40                  45              


Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu Pro Glu 
    50                  55                  60                  


Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu Ser Val 
65                  70                  75                  80  


Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly 
                85                  90                  95      


His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro 
            100                 105                 110         


Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly Ala Cys 
        115                 120                 125             


Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu 
    130                 135                 140                 


Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu Glu His 
145                 150                 155                 160 


Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val Met Ala 
                165                 170                 175     


Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser 
            180                 185                 190         


Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg Cys Val 
        195                 200                 205             


Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro Pro Asp 
    210                 215                 220                 


Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Lys Val Ala 
225                 230                 235                 240 


Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His Leu Asp 
                245                 250                 255     


Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln Ser Ser 
            260                 265                 270         


Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys Gly Val 
        275                 280                 285             


Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu Leu Thr 
    290                 295                 300                 


Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser 
305                 310                 315                 320 


Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg Arg Gln 
                325                 330                 335     


Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val Gly Ala 
            340                 345                 350         


Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln 
        355                 360                 365             


Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu 
    370                 375                 380                 


Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala Ala Val 
385                 390                 395                 400 


Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg Ala Ile 
                405                 410                 415     


Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr 
            420                 425                 430         


Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys 
        435                 440                 445             


Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met Asp Ser 
    450                 455                 460                 


Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn Ser Thr 
465                 470                 475                 480 


Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr 
                485                 490                 495     


Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala 
            500                 505                 510         


Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly Gly Arg 
        515                 520                 525             


Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro 
    530                 535                 540                 


Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu Thr Glu 
545                 550                 555                 560 


Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln 
                565                 570                 575     


Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly 
            580                 585                 590         


Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro 
        595                 600                 605             


Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp 
    610                 615                 620                 


Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630             


<210>  6
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated WT ADAMTS13

<400>  6
agatctgctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc       60

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      120

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      180

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      240

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      300

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg      360

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag      420

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg      480

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac      540

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc      600

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc      660

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgccgc      720

gtggccttcg gccccaaggc tgtcgcctgc accttcgcca gggagcacct ggatatgtgc      780

caggccctct cctgccacac agacccgctg gaccaaagca gctgcagccg cctcctcgtt      840

cctctcctgg atgggacaga atgtggcgtg gagaagtggt gctccaaggg tcgctgccgc      900

tccctggtgg agctgacccc catagcagca gtgcatgggc gctggtctag ctggggtccc      960

cgaagtcctt gctcccgctc ctgcggagga ggtgtggtca ccaggaggcg gcagtgcaac     1020

aaccccagac ctgcctttgg ggggcgtgca tgtgttggtg ctgacctcca ggccgagatg     1080

tgcaacactc aggcctgcga gaagacccag ctggagttca tgtcgcaaca gtgcgccagg     1140

accgacggcc agccgctgcg ctcctcccct ggcggcgcct ccttctacca ctggggtgct     1200

gctgtaccac acagccaagg ggatgctctg tgcagacaca tgtgccgggc cattggcgag     1260

agcttcatca tgaagcgtgg agacagcttc ctcgatggga cccggtgtat gccaagtggc     1320

ccccgggagg acgggaccct gagcctgtgt gtgtcgggca gctgcaggac atttggctgt     1380

gatggtagga tggactccca gcaggtatgg gacaggtgcc aggtgtgtgg tggggacaac     1440

agcacgtgca gcccacggaa gggctctttc acagctggca gagcgagaga atatgtcaca     1500

tttctgacag ttacccccaa cctgaccagt gtctacattg ccaaccacag gcctctcttc     1560

acacacttgg cggtgaggat cggagggcgc tatgtcgtgg ctgggaagat gagcatctcc     1620

cctaacacca cctacccctc cctcctggag gatggtcgtg tcgagtacag agtggccctc     1680

accgaggacc ggctgccccg cctggaggag atccgcatct ggggacccct ccaggaagat     1740

gctgacatcc aggtttacag gcggtatggc gaggagtatg gcaacctcac ccgcccagac     1800

atcaccttca cctacttcca gcctaagcca cggcaggccg actacaaaga ccatgacggt     1860

gattataaag atcatgatat cgattacaag gatgacgatg acaag                     1905


<210>  7
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated WT ADAMTS13

<400>  7

Arg Ser Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val 
1               5                   10                  15      


Gly Pro Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val 
            20                  25                  30          


Leu Thr Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu 
        35                  40                  45              


Gly Ala Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu 
    50                  55                  60                  


Pro Glu Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu 
65                  70                  75                  80  


Ser Val Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp 
                85                  90                  95      


Pro Gly His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu 
            100                 105                 110         


Leu Pro Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly 
        115                 120                 125             


Ala Cys Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe 
    130                 135                 140                 


Asp Leu Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu 
145                 150                 155                 160 


Glu His Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val 
                165                 170                 175     


Met Ala Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro 
            180                 185                 190         


Cys Ser Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg 
        195                 200                 205             


Cys Val Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro 
    210                 215                 220                 


Pro Asp Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Arg 
225                 230                 235                 240 


Val Ala Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His 
                245                 250                 255     


Leu Asp Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln 
            260                 265                 270         


Ser Ser Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys 
        275                 280                 285             


Gly Val Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu 
    290                 295                 300                 


Leu Thr Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro 
305                 310                 315                 320 


Arg Ser Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg 
                325                 330                 335     


Arg Gln Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val 
            340                 345                 350         


Gly Ala Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys 
        355                 360                 365             


Thr Gln Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln 
    370                 375                 380                 


Pro Leu Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala 
385                 390                 395                 400 


Ala Val Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg 
                405                 410                 415     


Ala Ile Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp 
            420                 425                 430         


Gly Thr Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser 
        435                 440                 445             


Leu Cys Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met 
    450                 455                 460                 


Asp Ser Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn 
465                 470                 475                 480 


Ser Thr Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg 
                485                 490                 495     


Glu Tyr Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr 
            500                 505                 510         


Ile Ala Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly 
        515                 520                 525             


Gly Arg Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr 
    530                 535                 540                 


Tyr Pro Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu 
545                 550                 555                 560 


Thr Glu Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro 
                565                 570                 575     


Leu Gln Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu 
            580                 585                 590         


Tyr Gly Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro 
        595                 600                 605             


Lys Pro Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp 
    610                 615                 620                 


His Asp Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630                 635 


<210>  8
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primers forward to mutate R312 to K

<400>  8
tacagcgcca acgagcagtg ccgcgtggcc ttcggcccca aggct                       45


<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer REVERSE to K

<400>  9
tacagcgcca acgagcagtg caaggtggcc ttcggcccca aggct                       45


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer AntiK

<400>  10
agccttgggg ccgaaggcca ccttgcactg ctcgttggcg ctgta                       45


<210>  11
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADAMTS-13 R312K

<400>  11

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Lys Val Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  12
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADAMTS-13 R312A

<400>  12

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Ala Val Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  13
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADAMTS-13 V313A

<400>  13

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Arg Ala Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  14
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADAMTS-13 V313D

<400>  14

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Arg Asp Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  15
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated R312A truncated

<400>  15

Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val Gly Pro 
1               5                   10                  15      


Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr 
            20                  25                  30          


Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala 
        35                  40                  45              


Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu Pro Glu 
    50                  55                  60                  


Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu Ser Val 
65                  70                  75                  80  


Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly 
                85                  90                  95      


His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro 
            100                 105                 110         


Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly Ala Cys 
        115                 120                 125             


Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu 
    130                 135                 140                 


Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu Glu His 
145                 150                 155                 160 


Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val Met Ala 
                165                 170                 175     


Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser 
            180                 185                 190         


Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg Cys Val 
        195                 200                 205             


Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro Pro Asp 
    210                 215                 220                 


Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Ala Val Ala 
225                 230                 235                 240 


Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His Leu Asp 
                245                 250                 255     


Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln Ser Ser 
            260                 265                 270         


Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys Gly Val 
        275                 280                 285             


Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu Leu Thr 
    290                 295                 300                 


Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser 
305                 310                 315                 320 


Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg Arg Gln 
                325                 330                 335     


Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val Gly Ala 
            340                 345                 350         


Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln 
        355                 360                 365             


Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu 
    370                 375                 380                 


Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala Ala Val 
385                 390                 395                 400 


Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg Ala Ile 
                405                 410                 415     


Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr 
            420                 425                 430         


Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys 
        435                 440                 445             


Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met Asp Ser 
    450                 455                 460                 


Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn Ser Thr 
465                 470                 475                 480 


Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr 
                485                 490                 495     


Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala 
            500                 505                 510         


Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly Gly Arg 
        515                 520                 525             


Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro 
    530                 535                 540                 


Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu Thr Glu 
545                 550                 555                 560 


Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln 
                565                 570                 575     


Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly 
            580                 585                 590         


Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro 
        595                 600                 605             


Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp 
    610                 615                 620                 


Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630             


<210>  16
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated V313A truncated

<400>  16

Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val Gly Pro 
1               5                   10                  15      


Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr 
            20                  25                  30          


Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala 
        35                  40                  45              


Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu Pro Glu 
    50                  55                  60                  


Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu Ser Val 
65                  70                  75                  80  


Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly 
                85                  90                  95      


His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro 
            100                 105                 110         


Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly Ala Cys 
        115                 120                 125             


Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu 
    130                 135                 140                 


Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu Glu His 
145                 150                 155                 160 


Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val Met Ala 
                165                 170                 175     


Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser 
            180                 185                 190         


Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg Cys Val 
        195                 200                 205             


Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro Pro Asp 
    210                 215                 220                 


Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Arg Ala Ala 
225                 230                 235                 240 


Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His Leu Asp 
                245                 250                 255     


Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln Ser Ser 
            260                 265                 270         


Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys Gly Val 
        275                 280                 285             


Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu Leu Thr 
    290                 295                 300                 


Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser 
305                 310                 315                 320 


Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg Arg Gln 
                325                 330                 335     


Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val Gly Ala 
            340                 345                 350         


Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln 
        355                 360                 365             


Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu 
    370                 375                 380                 


Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala Ala Val 
385                 390                 395                 400 


Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg Ala Ile 
                405                 410                 415     


Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr 
            420                 425                 430         


Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys 
        435                 440                 445             


Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met Asp Ser 
    450                 455                 460                 


Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn Ser Thr 
465                 470                 475                 480 


Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr 
                485                 490                 495     


Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala 
            500                 505                 510         


Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly Gly Arg 
        515                 520                 525             


Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro 
    530                 535                 540                 


Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu Thr Glu 
545                 550                 555                 560 


Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln 
                565                 570                 575     


Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly 
            580                 585                 590         


Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro 
        595                 600                 605             


Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp 
    610                 615                 620                 


Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630             


<210>  17
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated V313D truncated

<400>  17

Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val Gly Pro 
1               5                   10                  15      


Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr 
            20                  25                  30          


Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala 
        35                  40                  45              


Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu Pro Glu 
    50                  55                  60                  


Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu Ser Val 
65                  70                  75                  80  


Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly 
                85                  90                  95      


His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro 
            100                 105                 110         


Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly Ala Cys 
        115                 120                 125             


Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu 
    130                 135                 140                 


Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu Glu His 
145                 150                 155                 160 


Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val Met Ala 
                165                 170                 175     


Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser 
            180                 185                 190         


Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg Cys Val 
        195                 200                 205             


Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro Pro Asp 
    210                 215                 220                 


Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Arg Asp Ala 
225                 230                 235                 240 


Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His Leu Asp 
                245                 250                 255     


Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln Ser Ser 
            260                 265                 270         


Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys Gly Val 
        275                 280                 285             


Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu Leu Thr 
    290                 295                 300                 


Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser 
305                 310                 315                 320 


Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg Arg Gln 
                325                 330                 335     


Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val Gly Ala 
            340                 345                 350         


Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln 
        355                 360                 365             


Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu 
    370                 375                 380                 


Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala Ala Val 
385                 390                 395                 400 


Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg Ala Ile 
                405                 410                 415     


Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr 
            420                 425                 430         


Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys 
        435                 440                 445             


Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met Asp Ser 
    450                 455                 460                 


Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn Ser Thr 
465                 470                 475                 480 


Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr 
                485                 490                 495     


Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala 
            500                 505                 510         


Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly Gly Arg 
        515                 520                 525             


Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro 
    530                 535                 540                 


Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu Thr Glu 
545                 550                 555                 560 


Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln 
                565                 570                 575     


Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly 
            580                 585                 590         


Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro 
        595                 600                 605             


Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp 
    610                 615                 620                 


Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630             


<210>  18
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated mutated ADAMTS13 R312K

<400>  18
agatctgctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc       60

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      120

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      180

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      240

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      300

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg      360

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag      420

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg      480

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac      540

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc      600

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc      660

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgcaag      720

gtggccttcg gccccaaggc tgtcgcctgc accttcgcca gggagcacct ggatatgtgc      780

caggccctct cctgccacac agacccgctg gaccaaagca gctgcagccg cctcctcgtt      840

cctctcctgg atgggacaga atgtggcgtg gagaagtggt gctccaaggg tcgctgccgc      900

tccctggtgg agctgacccc catagcagca gtgcatgggc gctggtctag ctggggtccc      960

cgaagtcctt gctcccgctc ctgcggagga ggtgtggtca ccaggaggcg gcagtgcaac     1020

aaccccagac ctgcctttgg ggggcgtgca tgtgttggtg ctgacctcca ggccgagatg     1080

tgcaacactc aggcctgcga gaagacccag ctggagttca tgtcgcaaca gtgcgccagg     1140

accgacggcc agccgctgcg ctcctcccct ggcggcgcct ccttctacca ctggggtgct     1200

gctgtaccac acagccaagg ggatgctctg tgcagacaca tgtgccgggc cattggcgag     1260

agcttcatca tgaagcgtgg agacagcttc ctcgatggga cccggtgtat gccaagtggc     1320

ccccgggagg acgggaccct gagcctgtgt gtgtcgggca gctgcaggac atttggctgt     1380

gatggtagga tggactccca gcaggtatgg gacaggtgcc aggtgtgtgg tggggacaac     1440

agcacgtgca gcccacggaa gggctctttc acagctggca gagcgagaga atatgtcaca     1500

tttctgacag ttacccccaa cctgaccagt gtctacattg ccaaccacag gcctctcttc     1560

acacacttgg cggtgaggat cggagggcgc tatgtcgtgg ctgggaagat gagcatctcc     1620

cctaacacca cctacccctc cctcctggag gatggtcgtg tcgagtacag agtggccctc     1680

accgaggacc ggctgccccg cctggaggag atccgcatct ggggacccct ccaggaagat     1740

gctgacatcc aggtttacag gcggtatggc gaggagtatg gcaacctcac ccgcccagac     1800

atcaccttca cctacttcca gcctaagcca cggcaggccg actacaaaga ccatgacggt     1860

gattataaag atcatgatat cgattacaag gatgacgatg acaag                     1905


<210>  19
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated mutated ADAMTS13 R312-A

<400>  19
agatctgctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc       60

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      120

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      180

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      240

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      300

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg      360

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag      420

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg      480

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac      540

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc      600

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc      660

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgcgcg      720

gtggccttcg gccccaaggc tgtcgcctgc accttcgcca gggagcacct ggatatgtgc      780

caggccctct cctgccacac agacccgctg gaccaaagca gctgcagccg cctcctcgtt      840

cctctcctgg atgggacaga atgtggcgtg gagaagtggt gctccaaggg tcgctgccgc      900

tccctggtgg agctgacccc catagcagca gtgcatgggc gctggtctag ctggggtccc      960

cgaagtcctt gctcccgctc ctgcggagga ggtgtggtca ccaggaggcg gcagtgcaac     1020

aaccccagac ctgcctttgg ggggcgtgca tgtgttggtg ctgacctcca ggccgagatg     1080

tgcaacactc aggcctgcga gaagacccag ctggagttca tgtcgcaaca gtgcgccagg     1140

accgacggcc agccgctgcg ctcctcccct ggcggcgcct ccttctacca ctggggtgct     1200

gctgtaccac acagccaagg ggatgctctg tgcagacaca tgtgccgggc cattggcgag     1260

agcttcatca tgaagcgtgg agacagcttc ctcgatggga cccggtgtat gccaagtggc     1320

ccccgggagg acgggaccct gagcctgtgt gtgtcgggca gctgcaggac atttggctgt     1380

gatggtagga tggactccca gcaggtatgg gacaggtgcc aggtgtgtgg tggggacaac     1440

agcacgtgca gcccacggaa gggctctttc acagctggca gagcgagaga atatgtcaca     1500

tttctgacag ttacccccaa cctgaccagt gtctacattg ccaaccacag gcctctcttc     1560

acacacttgg cggtgaggat cggagggcgc tatgtcgtgg ctgggaagat gagcatctcc     1620

cctaacacca cctacccctc cctcctggag gatggtcgtg tcgagtacag agtggccctc     1680

accgaggacc ggctgccccg cctggaggag atccgcatct ggggacccct ccaggaagat     1740

gctgacatcc aggtttacag gcggtatggc gaggagtatg gcaacctcac ccgcccagac     1800

atcaccttca cctacttcca gcctaagcca cggcaggccg actacaaaga ccatgacggt     1860

gattataaag atcatgatat cgattacaag gatgacgatg acaag                     1905


<210>  20
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primers to mutate R312 to A

<400>  20
tacagcgcca acgagcagtg cgcggtggcc ttcggcccca aggct                       45


<210>  21
<211>  1908
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated mutated ADAMTS13 V313-D

<400>  21
agatctgctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc       60

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      120

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      180

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      240

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      300

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg      360

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag      420

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg      480

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac      540

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc      600

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc      660

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgccgc      720

aaggtggcct tcggccccaa ggctgtcgcc tgcaccttcg ccagggagca cctggatatg      780

tgccaggccc tctcctgcca cacagacccg ctggaccaaa gcagctgcag ccgcctcctc      840

gttcctctcc tggatgggac agaatgtggc gtggagaagt ggtgctccaa gggtcgctgc      900

cgctccctgg tggagctgac ccccatagca gcagtgcatg ggcgctggtc tagctggggt      960

ccccgaagtc cttgctcccg ctcctgcgga ggaggtgtgg tcaccaggag gcggcagtgc     1020

aacaacccca gacctgcctt tggggggcgt gcatgtgttg gtgctgacct ccaggccgag     1080

atgtgcaaca ctcaggcctg cgagaagacc cagctggagt tcatgtcgca acagtgcgcc     1140

aggaccgacg gccagccgct gcgctcctcc cctggcggcg cctccttcta ccactggggt     1200

gctgctgtac cacacagcca aggggatgct ctgtgcagac acatgtgccg ggccattggc     1260

gagagcttca tcatgaagcg tggagacagc ttcctcgatg ggacccggtg tatgccaagt     1320

ggcccccggg aggacgggac cctgagcctg tgtgtgtcgg gcagctgcag gacatttggc     1380

tgtgatggta ggatggactc ccagcaggta tgggacaggt gccaggtgtg tggtggggac     1440

aacagcacgt gcagcccacg gaagggctct ttcacagctg gcagagcgag agaatatgtc     1500

acatttctga cagttacccc caacctgacc agtgtctaca ttgccaacca caggcctctc     1560

ttcacacact tggcggtgag gatcggaggg cgctatgtcg tggctgggaa gatgagcatc     1620

tcccctaaca ccacctaccc ctccctcctg gaggatggtc gtgtcgagta cagagtggcc     1680

ctcaccgagg accggctgcc ccgcctggag gagatccgca tctggggacc cctccaggaa     1740

gatgctgaca tccaggttta caggcggtat ggcgaggagt atggcaacct cacccgccca     1800

gacatcacct tcacctactt ccagcctaag ccacggcagg ccgactacaa agaccatgac     1860

ggtgattata aagatcatga tatcgattac aaggatgacg atgacaag                  1908


<210>  22
<211>  1908
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated mutated ADAMTS13 V313-A

<400>  22
agatctgctg caggcggcat cctacacctg gagctgctgg tggccgtggg ccccgatgtc       60

ttccaggctc accaggagga cacagagcgc tatgtgctca ccaacctcaa catcggggca      120

gaactgcttc gggacccgtc cctgggggct cagtttcggg tgcacctggt gaagatggtc      180

attctgacag agcctgaggg tgctccaaat atcacagcca acctcacctc gtccctgctg      240

agcgtctgtg ggtggagcca gaccatcaac cctgaggacg acacggatcc tggccatgct      300

gacctggtcc tctatatcac taggtttgac ctggagttgc ctgatggtaa ccggcaggtg      360

cggggcgtca cccagctggg cggtgcctgc tccccaacct ggagctgcct cattaccgag      420

gacactggct tcgacctggg agtcaccatt gcccatgaga ttgggcacag cttcggcctg      480

gagcacgacg gcgcgcccgg cagcggctgc ggccccagcg gacacgtgat ggcttcggac      540

ggcgccgcgc cccgcgccgg cctcgcctgg tccccctgca gccgccggca gctgctgagc      600

ctgctcagcg caggacgggc gcgctgcgtg tgggacccgc cgcggcctca acccgggtcc      660

gcggggcacc cgccggatgc gcagcctggc ctctactaca gcgccaacga gcagtgccgc      720

gcggtggcct tcggccccaa ggctgtcgcc tgcaccttcg ccagggagca cctggatatg      780

tgccaggccc tctcctgcca cacagacccg ctggaccaaa gcagctgcag ccgcctcctc      840

gttcctctcc tggatgggac agaatgtggc gtggagaagt ggtgctccaa gggtcgctgc      900

cgctccctgg tggagctgac ccccatagca gcagtgcatg ggcgctggtc tagctggggt      960

ccccgaagtc cttgctcccg ctcctgcgga ggaggtgtgg tcaccaggag gcggcagtgc     1020

aacaacccca gacctgcctt tggggggcgt gcatgtgttg gtgctgacct ccaggccgag     1080

atgtgcaaca ctcaggcctg cgagaagacc cagctggagt tcatgtcgca acagtgcgcc     1140

aggaccgacg gccagccgct gcgctcctcc cctggcggcg cctccttcta ccactggggt     1200

gctgctgtac cacacagcca aggggatgct ctgtgcagac acatgtgccg ggccattggc     1260

gagagcttca tcatgaagcg tggagacagc ttcctcgatg ggacccggtg tatgccaagt     1320

ggcccccggg aggacgggac cctgagcctg tgtgtgtcgg gcagctgcag gacatttggc     1380

tgtgatggta ggatggactc ccagcaggta tgggacaggt gccaggtgtg tggtggggac     1440

aacagcacgt gcagcccacg gaagggctct ttcacagctg gcagagcgag agaatatgtc     1500

acatttctga cagttacccc caacctgacc agtgtctaca ttgccaacca caggcctctc     1560

ttcacacact tggcggtgag gatcggaggg cgctatgtcg tggctgggaa gatgagcatc     1620

tcccctaaca ccacctaccc ctccctcctg gaggatggtc gtgtcgagta cagagtggcc     1680

ctcaccgagg accggctgcc ccgcctggag gagatccgca tctggggacc cctccaggaa     1740

gatgctgaca tccaggttta caggcggtat ggcgaggagt atggcaacct cacccgccca     1800

gacatcacct tcacctactt ccagcctaag ccacggcagg ccgactacaa agaccatgac     1860

ggtgattata aagatcatga tatcgattac aaggatgacg atgacaag                  1908


<210>  23
<211>  1427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double mutant R312K, V313D (full length)

<400>  23

Met His Gln Arg His Pro Arg Ala Arg Cys Pro Pro Leu Cys Val Ala 
1               5                   10                  15      


Gly Ile Leu Ala Cys Gly Phe Leu Leu Gly Cys Trp Gly Pro Ser His 
            20                  25                  30          


Phe Gln Gln Ser Cys Leu Gln Ala Leu Glu Pro Gln Ala Val Ser Ser 
        35                  40                  45              


Tyr Leu Ser Pro Gly Ala Pro Leu Lys Gly Arg Pro Pro Ser Pro Gly 
    50                  55                  60                  


Phe Gln Arg Gln Arg Gln Arg Gln Arg Arg Ala Ala Gly Gly Ile Leu 
65                  70                  75                  80  


His Leu Glu Leu Leu Val Ala Val Gly Pro Asp Val Phe Gln Ala His 
                85                  90                  95      


Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr Asn Leu Asn Ile Gly Ala 
            100                 105                 110         


Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala Gln Phe Arg Val His Leu 
        115                 120                 125             


Val Lys Met Val Ile Leu Thr Glu Pro Glu Gly Ala Pro Asn Ile Thr 
    130                 135                 140                 


Ala Asn Leu Thr Ser Ser Leu Leu Ser Val Cys Gly Trp Ser Gln Thr 
145                 150                 155                 160 


Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly His Ala Asp Leu Val Leu 
                165                 170                 175     


Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro Asp Gly Asn Arg Gln Val 
            180                 185                 190         


Arg Gly Val Thr Gln Leu Gly Gly Ala Cys Ser Pro Thr Trp Ser Cys 
        195                 200                 205             


Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu Gly Val Thr Ile Ala His 
    210                 215                 220                 


Glu Ile Gly His Ser Phe Gly Leu Glu His Asp Gly Ala Pro Gly Ser 
225                 230                 235                 240 


Gly Cys Gly Pro Ser Gly His Val Met Ala Ser Asp Gly Ala Ala Pro 
                245                 250                 255     


Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser Arg Arg Gln Leu Leu Ser 
            260                 265                 270         


Leu Leu Ser Ala Gly Arg Ala Arg Cys Val Trp Asp Pro Pro Arg Pro 
        275                 280                 285             


Gln Pro Gly Ser Ala Gly His Pro Pro Asp Ala Gln Pro Gly Leu Tyr 
    290                 295                 300                 


Tyr Ser Ala Asn Glu Gln Cys Lys Asp Ala Phe Gly Pro Lys Ala Val 
305                 310                 315                 320 


Ala Cys Thr Phe Ala Arg Glu His Leu Asp Met Cys Gln Ala Leu Ser 
                325                 330                 335     


Cys His Thr Asp Pro Leu Asp Gln Ser Ser Cys Ser Arg Leu Leu Val 
            340                 345                 350         


Pro Leu Leu Asp Gly Thr Glu Cys Gly Val Glu Lys Trp Cys Ser Lys 
        355                 360                 365             


Gly Arg Cys Arg Ser Leu Val Glu Leu Thr Pro Ile Ala Ala Val His 
    370                 375                 380                 


Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser Pro Cys Ser Arg Ser Cys 
385                 390                 395                 400 


Gly Gly Gly Val Val Thr Arg Arg Arg Gln Cys Asn Asn Pro Arg Pro 
                405                 410                 415     


Ala Phe Gly Gly Arg Ala Cys Val Gly Ala Asp Leu Gln Ala Glu Met 
            420                 425                 430         


Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln Leu Glu Phe Met Ser Gln 
        435                 440                 445             


Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu Arg Ser Ser Pro Gly Gly 
    450                 455                 460                 


Ala Ser Phe Tyr His Trp Gly Ala Ala Val Pro His Ser Gln Gly Asp 
465                 470                 475                 480 


Ala Leu Cys Arg His Met Cys Arg Ala Ile Gly Glu Ser Phe Ile Met 
                485                 490                 495     


Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr Arg Cys Met Pro Ser Gly 
            500                 505                 510         


Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys Val Ser Gly Ser Cys Arg 
        515                 520                 525             


Thr Phe Gly Cys Asp Gly Arg Met Asp Ser Gln Gln Val Trp Asp Arg 
    530                 535                 540                 


Cys Gln Val Cys Gly Gly Asp Asn Ser Thr Cys Ser Pro Arg Lys Gly 
545                 550                 555                 560 


Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr Val Thr Phe Leu Thr Val 
                565                 570                 575     


Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala Asn His Arg Pro Leu Phe 
            580                 585                 590         


Thr His Leu Ala Val Arg Ile Gly Gly Arg Tyr Val Val Ala Gly Lys 
        595                 600                 605             


Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro Ser Leu Leu Glu Asp Gly 
    610                 615                 620                 


Arg Val Glu Tyr Arg Val Ala Leu Thr Glu Asp Arg Leu Pro Arg Leu 
625                 630                 635                 640 


Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln Glu Asp Ala Asp Ile Gln 
                645                 650                 655     


Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly Asn Leu Thr Arg Pro Asp 
            660                 665                 670         


Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro Arg Gln Ala Trp Val Trp 
        675                 680                 685             


Ala Ala Val Arg Gly Pro Cys Ser Val Ser Cys Gly Ala Gly Leu Arg 
    690                 695                 700                 


Trp Val Asn Tyr Ser Cys Leu Asp Gln Ala Arg Lys Glu Leu Val Glu 
705                 710                 715                 720 


Thr Val Gln Cys Gln Gly Ser Gln Gln Pro Pro Ala Trp Pro Glu Ala 
                725                 730                 735     


Cys Val Leu Glu Pro Cys Pro Pro Tyr Trp Ala Val Gly Asp Phe Gly 
            740                 745                 750         


Pro Cys Ser Ala Ser Cys Gly Gly Gly Leu Arg Glu Arg Pro Val Arg 
        755                 760                 765             


Cys Val Glu Ala Gln Gly Ser Leu Leu Lys Thr Leu Pro Pro Ala Arg 
    770                 775                 780                 


Cys Arg Ala Gly Ala Gln Gln Pro Ala Val Ala Leu Glu Thr Cys Asn 
785                 790                 795                 800 


Pro Gln Pro Cys Pro Ala Arg Trp Glu Val Ser Glu Pro Ser Ser Cys 
                805                 810                 815     


Thr Ser Ala Gly Gly Ala Gly Leu Ala Leu Glu Asn Glu Thr Cys Val 
            820                 825                 830         


Pro Gly Ala Asp Gly Leu Glu Ala Pro Val Thr Glu Gly Pro Gly Ser 
        835                 840                 845             


Val Asp Glu Lys Leu Pro Ala Pro Glu Pro Cys Val Gly Met Ser Cys 
    850                 855                 860                 


Pro Pro Gly Trp Gly His Leu Asp Ala Thr Ser Ala Gly Glu Lys Ala 
865                 870                 875                 880 


Pro Ser Pro Trp Gly Ser Ile Arg Thr Gly Ala Gln Ala Ala His Val 
                885                 890                 895     


Trp Thr Pro Ala Ala Gly Ser Cys Ser Val Ser Cys Gly Arg Gly Leu 
            900                 905                 910         


Met Glu Leu Arg Phe Leu Cys Met Asp Ser Ala Leu Arg Val Pro Val 
        915                 920                 925             


Gln Glu Glu Leu Cys Gly Leu Ala Ser Lys Pro Gly Ser Arg Arg Glu 
    930                 935                 940                 


Val Cys Gln Ala Val Pro Cys Pro Ala Arg Trp Gln Tyr Lys Leu Ala 
945                 950                 955                 960 


Ala Cys Ser Val Ser Cys Gly Arg Gly Val Val Arg Arg Ile Leu Tyr 
                965                 970                 975     


Cys Ala Arg Ala His Gly Glu Asp Asp Gly Glu Glu Ile Leu Leu Asp 
            980                 985                 990         


Thr Gln Cys Gln Gly Leu Pro Arg  Pro Glu Pro Gln Glu  Ala Cys Ser 
        995                 1000                 1005             


Leu Glu  Pro Cys Pro Pro Arg  Trp Lys Val Met Ser  Leu Gly Pro 
    1010                 1015                 1020             


Cys Ser  Ala Ser Cys Gly Leu  Gly Thr Ala Arg Arg  Ser Val Ala 
    1025                 1030                 1035             


Cys Val  Gln Leu Asp Gln Gly  Gln Asp Val Glu Val  Asp Glu Ala 
    1040                 1045                 1050             


Ala Cys  Ala Ala Leu Val Arg  Pro Glu Ala Ser Val  Pro Cys Leu 
    1055                 1060                 1065             


Ile Ala  Asp Cys Thr Tyr Arg  Trp His Val Gly Thr  Trp Met Glu 
    1070                 1075                 1080             


Cys Ser  Val Ser Cys Gly Asp  Gly Ile Gln Arg Arg  Arg Asp Thr 
    1085                 1090                 1095             


Cys Leu  Gly Pro Gln Ala Gln  Ala Pro Val Pro Ala  Asp Phe Cys 
    1100                 1105                 1110             


Gln His  Leu Pro Lys Pro Val  Thr Val Arg Gly Cys  Trp Ala Gly 
    1115                 1120                 1125             


Pro Cys  Val Gly Gln Gly Thr  Pro Ser Leu Val Pro  His Glu Glu 
    1130                 1135                 1140             


Ala Ala  Ala Pro Gly Arg Thr  Thr Ala Thr Pro Ala  Gly Ala Ser 
    1145                 1150                 1155             


Leu Glu  Trp Ser Gln Ala Arg  Gly Leu Leu Phe Ser  Pro Ala Pro 
    1160                 1165                 1170             


Gln Pro  Arg Arg Leu Leu Pro  Gly Pro Gln Glu Asn  Ser Val Gln 
    1175                 1180                 1185             


Ser Ser  Ala Cys Gly Arg Gln  His Leu Glu Pro Thr  Gly Thr Ile 
    1190                 1195                 1200             


Asp Met  Arg Gly Pro Gly Gln  Ala Asp Cys Ala Val  Ala Ile Gly 
    1205                 1210                 1215             


Arg Pro  Leu Gly Glu Val Val  Thr Leu Arg Val Leu  Glu Ser Ser 
    1220                 1225                 1230             


Leu Asn  Cys Ser Ala Gly Asp  Met Leu Leu Leu Trp  Gly Arg Leu 
    1235                 1240                 1245             


Thr Trp  Arg Lys Met Cys Arg  Lys Leu Leu Asp Met  Thr Phe Ser 
    1250                 1255                 1260             


Ser Lys  Thr Asn Thr Leu Val  Val Arg Gln Arg Cys  Gly Arg Pro 
    1265                 1270                 1275             


Gly Gly  Gly Val Leu Leu Arg  Tyr Gly Ser Gln Leu  Ala Pro Glu 
    1280                 1285                 1290             


Thr Phe  Tyr Arg Glu Cys Asp  Met Gln Leu Phe Gly  Pro Trp Gly 
    1295                 1300                 1305             


Glu Ile  Val Ser Pro Ser Leu  Ser Pro Ala Thr Ser  Asn Ala Gly 
    1310                 1315                 1320             


Gly Cys  Arg Leu Phe Ile Asn  Val Ala Pro His Ala  Arg Ile Ala 
    1325                 1330                 1335             


Ile His  Ala Leu Ala Thr Asn  Met Gly Ala Gly Thr  Glu Gly Ala 
    1340                 1345                 1350             


Asn Ala  Ser Tyr Ile Leu Ile  Arg Asp Thr His Ser  Leu Arg Thr 
    1355                 1360                 1365             


Thr Ala  Phe His Gly Gln Gln  Val Leu Tyr Trp Glu  Ser Glu Ser 
    1370                 1375                 1380             


Ser Gln  Ala Glu Met Glu Phe  Ser Glu Gly Phe Leu  Lys Ala Gln 
    1385                 1390                 1395             


Ala Ser  Leu Arg Gly Gln Tyr  Trp Thr Leu Gln Ser  Trp Val Pro 
    1400                 1405                 1410             


Glu Met  Gln Asp Pro Gln Ser  Trp Lys Gly Lys Glu  Gly Thr 
    1415                 1420                 1425         


<210>  24
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Double mutant R312K, V313D truncated

<400>  24

Ala Ala Gly Gly Ile Leu His Leu Glu Leu Leu Val Ala Val Gly Pro 
1               5                   10                  15      


Asp Val Phe Gln Ala His Gln Glu Asp Thr Glu Arg Tyr Val Leu Thr 
            20                  25                  30          


Asn Leu Asn Ile Gly Ala Glu Leu Leu Arg Asp Pro Ser Leu Gly Ala 
        35                  40                  45              


Gln Phe Arg Val His Leu Val Lys Met Val Ile Leu Thr Glu Pro Glu 
    50                  55                  60                  


Gly Ala Pro Asn Ile Thr Ala Asn Leu Thr Ser Ser Leu Leu Ser Val 
65                  70                  75                  80  


Cys Gly Trp Ser Gln Thr Ile Asn Pro Glu Asp Asp Thr Asp Pro Gly 
                85                  90                  95      


His Ala Asp Leu Val Leu Tyr Ile Thr Arg Phe Asp Leu Glu Leu Pro 
            100                 105                 110         


Asp Gly Asn Arg Gln Val Arg Gly Val Thr Gln Leu Gly Gly Ala Cys 
        115                 120                 125             


Ser Pro Thr Trp Ser Cys Leu Ile Thr Glu Asp Thr Gly Phe Asp Leu 
    130                 135                 140                 


Gly Val Thr Ile Ala His Glu Ile Gly His Ser Phe Gly Leu Glu His 
145                 150                 155                 160 


Asp Gly Ala Pro Gly Ser Gly Cys Gly Pro Ser Gly His Val Met Ala 
                165                 170                 175     


Ser Asp Gly Ala Ala Pro Arg Ala Gly Leu Ala Trp Ser Pro Cys Ser 
            180                 185                 190         


Arg Arg Gln Leu Leu Ser Leu Leu Ser Ala Gly Arg Ala Arg Cys Val 
        195                 200                 205             


Trp Asp Pro Pro Arg Pro Gln Pro Gly Ser Ala Gly His Pro Pro Asp 
    210                 215                 220                 


Ala Gln Pro Gly Leu Tyr Tyr Ser Ala Asn Glu Gln Cys Lys Asp Ala 
225                 230                 235                 240 


Phe Gly Pro Lys Ala Val Ala Cys Thr Phe Ala Arg Glu His Leu Asp 
                245                 250                 255     


Met Cys Gln Ala Leu Ser Cys His Thr Asp Pro Leu Asp Gln Ser Ser 
            260                 265                 270         


Cys Ser Arg Leu Leu Val Pro Leu Leu Asp Gly Thr Glu Cys Gly Val 
        275                 280                 285             


Glu Lys Trp Cys Ser Lys Gly Arg Cys Arg Ser Leu Val Glu Leu Thr 
    290                 295                 300                 


Pro Ile Ala Ala Val His Gly Arg Trp Ser Ser Trp Gly Pro Arg Ser 
305                 310                 315                 320 


Pro Cys Ser Arg Ser Cys Gly Gly Gly Val Val Thr Arg Arg Arg Gln 
                325                 330                 335     


Cys Asn Asn Pro Arg Pro Ala Phe Gly Gly Arg Ala Cys Val Gly Ala 
            340                 345                 350         


Asp Leu Gln Ala Glu Met Cys Asn Thr Gln Ala Cys Glu Lys Thr Gln 
        355                 360                 365             


Leu Glu Phe Met Ser Gln Gln Cys Ala Arg Thr Asp Gly Gln Pro Leu 
    370                 375                 380                 


Arg Ser Ser Pro Gly Gly Ala Ser Phe Tyr His Trp Gly Ala Ala Val 
385                 390                 395                 400 


Pro His Ser Gln Gly Asp Ala Leu Cys Arg His Met Cys Arg Ala Ile 
                405                 410                 415     


Gly Glu Ser Phe Ile Met Lys Arg Gly Asp Ser Phe Leu Asp Gly Thr 
            420                 425                 430         


Arg Cys Met Pro Ser Gly Pro Arg Glu Asp Gly Thr Leu Ser Leu Cys 
        435                 440                 445             


Val Ser Gly Ser Cys Arg Thr Phe Gly Cys Asp Gly Arg Met Asp Ser 
    450                 455                 460                 


Gln Gln Val Trp Asp Arg Cys Gln Val Cys Gly Gly Asp Asn Ser Thr 
465                 470                 475                 480 


Cys Ser Pro Arg Lys Gly Ser Phe Thr Ala Gly Arg Ala Arg Glu Tyr 
                485                 490                 495     


Val Thr Phe Leu Thr Val Thr Pro Asn Leu Thr Ser Val Tyr Ile Ala 
            500                 505                 510         


Asn His Arg Pro Leu Phe Thr His Leu Ala Val Arg Ile Gly Gly Arg 
        515                 520                 525             


Tyr Val Val Ala Gly Lys Met Ser Ile Ser Pro Asn Thr Thr Tyr Pro 
    530                 535                 540                 


Ser Leu Leu Glu Asp Gly Arg Val Glu Tyr Arg Val Ala Leu Thr Glu 
545                 550                 555                 560 


Asp Arg Leu Pro Arg Leu Glu Glu Ile Arg Ile Trp Gly Pro Leu Gln 
                565                 570                 575     


Glu Asp Ala Asp Ile Gln Val Tyr Arg Arg Tyr Gly Glu Glu Tyr Gly 
            580                 585                 590         


Asn Leu Thr Arg Pro Asp Ile Thr Phe Thr Tyr Phe Gln Pro Lys Pro 
        595                 600                 605             


Arg Gln Ala Asp Tyr Lys Asp His Asp Gly Asp Tyr Lys Asp His Asp 
    610                 615                 620                 


Ile Asp Tyr Lys Asp Asp Asp Asp Lys 
625                 630             


