                         SEQUENCE LISTING

<110>  Nuclera Nucleics Ltd
 
<120>  Methods relating to de novo enzymatic nucleic acid synthesis

<130>  P33070WO1

<140>  PCT/GB2021/052095
<141>  2021-08-12

<150>  GB2012544.9
<151>  2020-08-12

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  494
<212>  PRT
<213>  Lepisosteus oculatus

<400>  1

Met Leu His Ile Pro Ile Phe Pro Pro Ile Lys Lys Arg Gln Lys Leu 
1               5                   10                  15      


Pro Glu Ser Arg Asn Ser Cys Lys Tyr Glu Val Lys Phe Ser Glu Val 
            20                  25                  30          


Ala Ile Phe Leu Val Glu Arg Lys Met Gly Ser Ser Arg Arg Lys Phe 
        35                  40                  45              


Leu Thr Asn Leu Ala Arg Ser Lys Gly Phe Arg Ile Glu Asp Val Leu 
    50                  55                  60                  


Ser Asp Ala Val Thr His Val Val Ala Glu Asp Asn Ser Ala Asp Glu 
65                  70                  75                  80  


Leu Trp Gln Trp Leu Gln Asn Ser Ser Leu Gly Asp Leu Ser Lys Ile 
                85                  90                  95      


Glu Val Leu Asp Ile Ser Trp Phe Thr Glu Cys Met Gly Ala Gly Lys 
            100                 105                 110         


Pro Val Gln Val Glu Ala Arg His Cys Leu Val Lys Ser Cys Pro Val 
        115                 120                 125             


Ile Asp Gln Tyr Leu Glu Pro Ser Thr Val Glu Thr Val Ser Gln Tyr 
    130                 135                 140                 


Ala Cys Gln Arg Arg Thr Thr Met Glu Asn His Asn Gln Ile Phe Thr 
145                 150                 155                 160 


Asp Ala Phe Ala Ile Leu Ala Glu Asn Ala Glu Phe Asn Glu Ser Glu 
                165                 170                 175     


Gly Pro Cys Leu Ala Phe Met Arg Ala Ala Ser Leu Leu Lys Ser Leu 
            180                 185                 190         


Pro His Ala Ile Ser Ser Ser Lys Asp Leu Glu Gly Leu Pro Cys Leu 
        195                 200                 205             


Gly Asp Gln Thr Lys Ala Val Ile Glu Asp Ile Leu Glu Tyr Gly Gln 
    210                 215                 220                 


Cys Ser Lys Val Gln Asp Val Leu Cys Asp Asp Arg Tyr Gln Thr Ile 
225                 230                 235                 240 


Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys Thr Ala Glu Lys 
                245                 250                 255     


Trp Tyr Arg Lys Gly Phe His Ser Leu Glu Glu Val Gln Ala Asp Asn 
            260                 265                 270         


Ala Ile His Phe Thr Lys Met Gln Lys Ala Gly Phe Leu Tyr Tyr Asp 
        275                 280                 285             


Asp Ile Ser Ala Ala Val Cys Lys Ala Glu Ala Gln Ala Ile Gly Gln 
    290                 295                 300                 


Ile Val Glu Glu Thr Val Arg Leu Ile Ala Pro Asp Ala Ile Val Thr 
305                 310                 315                 320 


Leu Thr Gly Gly Phe Arg Arg Gly Lys Glu Cys Gly His Asp Val Asp 
                325                 330                 335     


Phe Leu Ile Thr Thr Pro Glu Met Gly Lys Glu Val Trp Leu Leu Asn 
            340                 345                 350         


Arg Leu Ile Asn Arg Leu Gln Asn Gln Gly Ile Leu Leu Tyr Tyr Asp 
        355                 360                 365             


Ile Val Glu Ser Thr Phe Asp Lys Thr Arg Leu Pro Cys Arg Lys Phe 
    370                 375                 380                 


Glu Ala Met Asp His Phe Gln Lys Cys Phe Ala Ile Ile Lys Leu Lys 
385                 390                 395                 400 


Lys Glu Leu Ala Ala Gly Arg Val Gln Lys Asp Trp Lys Ala Ile Arg 
                405                 410                 415     


Val Asp Phe Val Ala Pro Pro Val Asp Asn Phe Ala Phe Ala Leu Leu 
            420                 425                 430         


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Phe Ala 
        435                 440                 445             


Arg His Glu Arg Lys Met Leu Leu Asp Asn His Ala Leu Tyr Asp Lys 
    450                 455                 460                 


Thr Lys Lys Ile Phe Leu Pro Ala Lys Thr Glu Glu Asp Ile Phe Ala 
465                 470                 475                 480 


His Leu Gly Leu Asp Tyr Ile Asp Pro Trp Gln Arg Asn Ala 
                485                 490                 


<210>  2
<211>  355
<212>  PRT
<213>  Lepisosteus oculatus

<400>  2

Thr Val Ser Gln Tyr Ala Cys Gln Arg Arg Thr Thr Met Glu Asn His 
1               5                   10                  15      


Asn Gln Ile Phe Thr Asp Ala Phe Ala Ile Leu Ala Glu Asn Ala Glu 
            20                  25                  30          


Phe Asn Glu Ser Glu Gly Pro Cys Leu Ala Phe Met Arg Ala Ala Ser 
        35                  40                  45              


Leu Leu Lys Ser Leu Pro His Ala Ile Ser Ser Ser Lys Asp Leu Glu 
    50                  55                  60                  


Gly Leu Pro Cys Leu Gly Asp Gln Thr Lys Ala Val Ile Glu Asp Ile 
65                  70                  75                  80  


Leu Glu Tyr Gly Gln Cys Ser Lys Val Gln Asp Val Leu Cys Asp Asp 
                85                  90                  95      


Arg Tyr Gln Thr Ile Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu 
            100                 105                 110         


Lys Thr Ala Glu Lys Trp Tyr Arg Lys Gly Phe His Ser Leu Glu Glu 
        115                 120                 125             


Val Gln Ala Asp Asn Ala Ile His Phe Thr Lys Met Gln Lys Ala Gly 
    130                 135                 140                 


Phe Leu Tyr Tyr Asp Asp Ile Ser Ala Ala Val Cys Lys Ala Glu Ala 
145                 150                 155                 160 


Gln Ala Ile Gly Gln Ile Val Glu Glu Thr Val Arg Leu Ile Ala Pro 
                165                 170                 175     


Asp Ala Ile Val Thr Leu Thr Gly Gly Phe Arg Arg Gly Lys Glu Cys 
            180                 185                 190         


Gly His Asp Val Asp Phe Leu Ile Thr Thr Pro Glu Met Gly Lys Glu 
        195                 200                 205             


Val Trp Leu Leu Asn Arg Leu Ile Asn Arg Leu Gln Asn Gln Gly Ile 
    210                 215                 220                 


Leu Leu Tyr Tyr Asp Ile Val Glu Ser Thr Phe Asp Lys Thr Arg Leu 
225                 230                 235                 240 


Pro Cys Arg Lys Phe Glu Ala Met Asp His Phe Gln Lys Cys Phe Ala 
                245                 250                 255     


Ile Ile Lys Leu Lys Lys Glu Leu Ala Ala Gly Arg Val Gln Lys Asp 
            260                 265                 270         


Trp Lys Ala Ile Arg Val Asp Phe Val Ala Pro Pro Val Asp Asn Phe 
        275                 280                 285             


Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp 
    290                 295                 300                 


Leu Arg Arg Phe Ala Arg His Glu Arg Lys Met Leu Leu Asp Asn His 
305                 310                 315                 320 


Ala Leu Tyr Asp Lys Thr Lys Lys Ile Phe Leu Pro Ala Lys Thr Glu 
                325                 330                 335     


Glu Asp Ile Phe Ala His Leu Gly Leu Asp Tyr Ile Asp Pro Trp Gln 
            340                 345                 350         


Arg Asn Ala 
        355 


<210>  3
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  3

Glu Ser Thr Phe Glu Lys Leu Arg Leu Pro Ser Arg Lys Val Asp Ala 
1               5                   10                  15      


Leu Asp His Phe 
            20  


<210>  4
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  4

His Ser Cys Cys Glu Ser Pro Thr Arg Leu Ala Gln Gln Ser His Met 
1               5                   10                  15      


Asp Ala Phe 
            


<210>  5
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo D649 seq


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  An internal phosphorothioate bond is located between residues 1 
       and 2; 2 and 3; 3 and 4.

<400>  5
tttttttttt tugagtcctc agcacacgac gctcttccga tctagaagac tt               52


<210>  6
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo D412 seq


<220>
<221>  misc_feature
<222>  (69)..(70)
<223>  An internal phosphorothioate bond is located between residues 69 
       and 70.

<400>  6
aatgatacgg cgaccaccga gatctacacc acagactaca ctctttccct acacgacgct       60

cttccgatct                                                              70


<210>  7
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo D413 seq


<220>
<221>  misc_feature
<222>  (69)..(70)
<223>  An internal phosphorothioate bond is located between residues 69 
       and 70.

<400>  7
aatgatacgg cgaccaccga gatctacacc gacacttaca ctctttccct acacgacgct       60

cttccgatct                                                              70


<210>  8
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo D470 seq


<220>
<221>  misc_feature
<222>  (52)..(53)
<223>  An internal phosporothioate bond is located between residues 52 
       and 53.

<400>  8
caagcagaag acggcatacg agatagacat gcgtgactgg agttcagacg tgt              53


<210>  9
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligo D475 seq


<220>
<221>  misc_feature
<222>  (52)..(53)
<223>  An internal phosphorothioate bond is located between residues 52 
       and 53.

<400>  9
caagcagaag acggcatacg agatgccttc ttgtgactgg agttcagacg tgt              53


