               SEQUENCE LISTING

<110> Universität Würzburg

<120> Siglec-6-binding polypeptides

<130> 225871

<160> 46

<170> BiSSAP 1.3.6

<210> 1
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> GMCSF signal peptide

<400> 1
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro 
            20          

<210> 2
<211> 51
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 1

<400> 2
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc c              51


<210> 3
<211> 116
<212> PRT
<213> Artificial Sequence


<220> 
<223> JML-1 heavy chain variable domain (VH)

<400> 3
Lys Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          
Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp Gly Gln Gly Thr Met Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210> 4
<211> 348
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 3

<400> 4
aaggtgcagc tgctggaatc tggcggagga ctggttcagc ctggcagaag cctgagactg      60

tcttgtgccg ccagcggctt caccttcgac gattatggca tgcactgggt ccgacaggcc     120

cctggcaaag gacttgaatg ggtgtccggc atcagctgga acagcggctc tatcggctac     180

gccgattccg tgaagggcag attcaccatc agccgggaca acagcaagaa caccctgtac     240

ctgcagatga acagcctgag agccgaggac accgccgtgt actattgtgc tagaggcggc     300

cagaccatcg acatctgggg acagggaacc atggtcaccg tttctagc                  348


<210> 5
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4(GS)x3 linker

<400> 5
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  

<210> 6
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 5

<400> 6
ggaggcggag gttctggcgg cggaggaagt ggtggcggag gctct                     45


<210> 7
<211> 107
<212> PRT
<213> Artificial Sequence


<220> 
<223> JML-1 light chain variable domain (VL)

<400> 7
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105         

<210> 8
<211> 321
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 7

<400> 8
gatatccaga tgacacagag ccccagcagc ctgtctgcct ctgtgggaga cagagtgacc      60

atcacctgta gagccagcca gagcatcagc agctacctga actggtatca gcaaaagccc     120

ggcaaggccc ctaaactgct gatctacgct gcctccagtc tgcagagcgg agtgcctagc     180

agattttctg gctctggcag cggcaccgac ttcaccctga ccatatctag cctgcagcct     240

gaggacttcg ccacctacta ctgccagcag agctacagca cccctttcac atttggccct     300

ggcaccaagg tggacatcaa a                                               321


<210> 9
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> IgG4 hinge domain

<400> 9
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          

<210> 10
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 9

<400> 10
gagtctaagt acggaccgcc ttgtcctcct tgtcca                               36


<210> 11
<211> 32
<212> PRT
<213> Artificial Sequence


<220> 
<223> IgG3 hinge

<400> 11
Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys 
1               5                   10                  15      
Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
            20                  25                  30          



<210> 12
<211> 96
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 11

<400> 12
gagctgaaaa cccctctggg cgacaccaca cacacatgcc ctagatgtcc ggaacccaag     60

agctgcgata cccccccacc ttgccccaga tgcccc                               96


<210> 13
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28 transmembrane domain

<400> 13
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              

<210> 14
<211> 84
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 13

<400> 14
atgttttggg tgctggtggt cgtgggcgga gtgctggcct gttacagcct gctcgtgacc     60

gtggccttca tcatcttttg ggtc                                            84


<210> 15
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28 costimulatory domain

<400> 15
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 16
<211> 123
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 15

<400> 16
cgcagcaagc ggagcagagg cggccacagc gactacatga acatgacccc tagacggcct     60

ggccccacca gaaagcacta ccagccctac gcccctcccc gggactttgc cgcctacaga    120

agc                                                                  123


<210> 17
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4-1BB costimulatory domain

<400> 17
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          

<210> 18
<211> 126
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 17

<400> 18
aagcggggca gaaagaagct gctgtacatc tttaagcagc ccttcatgcg gcccgtgcag     60

accacccagg aagaggacgg ctgctcctgc agattccccg aggaagaaga aggcggctgc    120

gagctg                                                               126


<210> 19
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3zeta signaling domain

<400> 19
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         



<210> 20
<211> 336
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 19

<400> 20
cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca gaatcagctg      60

tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa gcggagaggc     120

cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg cctgtataac     180

gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa gggcgagcgg     240

aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac caaggatacc     300

tacgacgccc tgcacatgca ggccctgccc ccaagg                               336


<210> 21
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> T2A ribosomal skipping sequence

<400> 21
Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      
Val Glu Glu Asn Pro Gly Pro Arg 
            20                  

<210> 22
<211> 72
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 21

<400> 22
ctcgagggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat     60

cccggcccta gg                                                         72


<210> 23
<211> 335
<212> PRT
<213> Artificial Sequence


<220> 
<223> EGFRt

<400> 23
Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      
Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          
Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              
Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  
Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  
Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      
Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         
Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             
Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 
Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 
Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     
Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         
Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             
Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 
Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 
Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     
His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         
Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             
Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 
Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 
Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 

<210> 24
<211> 1008
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 23

<400> 24
cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct      60

acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg     120

gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat     180

attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac     240

aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat     300

ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc     360

aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat     420

acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac     480

agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag     540

ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg     600

gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct     660

gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga     720

cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag     780

acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc     840

ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt     900

gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc     960

ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgtga                 1008


<210> 25
<211> 238
<212> PRT
<213> Artificial Sequence


<220> 
<223> JML-1 scFv

<400> 25
Lys Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr 
            20                  25                  30          
Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile Gly Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp Gly Gln Gly Thr Met Val 
            100                 105                 110         
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             
Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
    130                 135                 140                 
Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile 
145                 150                 155                 160 
Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
                165                 170                 175     
Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg 
            180                 185                 190         
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
        195                 200                 205             
Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser 
    210                 215                 220                 
Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
225                 230                 235             

<210> 26
<211> 714
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 25

<400> 26
aaggtgcagc tgctggaatc tggcggagga ctggttcagc ctggcagaag cctgagactg      60

tcttgtgccg ccagcggctt caccttcgac gattatggca tgcactgggt ccgacaggcc     120

cctggcaaag gacttgaatg ggtgtccggc atcagctgga acagcggctc tatcggctac     180

gccgattccg tgaagggcag attcaccatc agccgggaca acagcaagaa caccctgtac     240

ctgcagatga acagcctgag agccgaggac accgccgtgt actattgtgc tagaggcggc     300

cagaccatcg acatctgggg acagggaacc atggtcaccg tttctagcgg aggcggaggt     360

tctggcggcg gaggaagtgg tggcggaggc tctgatatcc agatgacaca gagccccagc     420

agcctgtctg cctctgtggg agacagagtg accatcacct gtagagccag ccagagcatc     480

agcagctacc tgaactggta tcagcaaaag cccggcaagg cccctaaact gctgatctac     540

gctgcctcca gtctgcagag cggagtgcct agcagatttt ctggctctgg cagcggcacc     600

gacttcaccc tgaccatatc tagcctgcag cctgaggact tcgccaccta ctactgccag     660

cagagctaca gcaccccttt cacatttggc cctggcacca aggtggacat caaa           714


<210> 27
<211> 453
<212> PRT
<213> Artificial Sequence


<220> 
<223> full-length CAR with IgG4 hinge and CD28 costimulatory domain

<400> 27
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
            260                 265                 270         
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
        275                 280                 285             
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
    290                 295                 300                 
Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
305                 310                 315                 320 
Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
                325                 330                 335     
Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
            340                 345                 350         
Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
        355                 360                 365             
Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
    370                 375                 380                 
Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
385                 390                 395                 400 
Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
                405                 410                 415     
Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
            420                 425                 430         
Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
        435                 440                 445             
Ala Leu Pro Pro Arg 
    450             

<210> 28
<211> 714
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 27

<400> 28
aaggtgcagc tgctggaatc tggcggagga ctggttcagc ctggcagaag cctgagactg      60

tcttgtgccg ccagcggctt caccttcgac gattatggca tgcactgggt ccgacaggcc     120

cctggcaaag gacttgaatg ggtgtccggc atcagctgga acagcggctc tatcggctac     180

gccgattccg tgaagggcag attcaccatc agccgggaca acagcaagaa caccctgtac     240

ctgcagatga acagcctgag agccgaggac accgccgtgt actattgtgc tagaggcggc     300

cagaccatcg acatctgggg acagggaacc atggtcaccg tttctagcgg aggcggaggt     360

tctggcggcg gaggaagtgg tggcggaggc tctgatatcc agatgacaca gagccccagc     420

agcctgtctg cctctgtggg agacagagtg accatcacct gtagagccag ccagagcatc     480

agcagctacc tgaactggta tcagcaaaag cccggcaagg cccctaaact gctgatctac     540

gctgcctcca gtctgcagag cggagtgcct agcagatttt ctggctctgg cagcggcacc     600

gacttcaccc tgaccatatc tagcctgcag cctgaggact tcgccaccta ctactgccag     660

cagagctaca gcaccccttt cacatttggc cctggcacca aggtggacat caaa           714


<210> 29
<211> 454
<212> PRT
<213> Artificial Sequence


<220> 
<223> full-length CAR with IgG4 hinge and 4-1BB costimulatory domain

<400> 29
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
            260                 265                 270         
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
        275                 280                 285             
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg 
    290                 295                 300                 
Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
305                 310                 315                 320 
Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
                325                 330                 335     
Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
            340                 345                 350         
Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
        355                 360                 365             
Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
    370                 375                 380                 
Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
385                 390                 395                 400 
Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
                405                 410                 415     
Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
            420                 425                 430         
Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
        435                 440                 445             
Gln Ala Leu Pro Pro Arg 
    450                 

<210> 30
<211> 1362
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 29

<400> 30
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg      60

atccctaagg tgcagctgct ggaatctggc ggaggactgg ttcagcctgg cagaagcctg     120

agactgtctt gtgccgccag cggcttcacc ttcgacgatt atggcatgca ctgggtccga     180

caggcccctg gcaaaggact tgaatgggtg tccggcatca gctggaacag cggctctatc     240

ggctacgccg attccgtgaa gggcagattc accatcagcc gggacaacag caagaacacc     300

ctgtacctgc agatgaacag cctgagagcc gaggacaccg ccgtgtacta ttgtgctaga     360

ggcggccaga ccatcgacat ctggggacag ggaaccatgg tcaccgtttc tagcggaggc     420

ggaggttctg gcggcggagg aagtggtggc ggaggctctg atatccagat gacacagagc     480

cccagcagcc tgtctgcctc tgtgggagac agagtgacca tcacctgtag agccagccag     540

agcatcagca gctacctgaa ctggtatcag caaaagcccg gcaaggcccc taaactgctg     600

atctacgctg cctccagtct gcagagcgga gtgcctagca gattttctgg ctctggcagc     660

ggcaccgact tcaccctgac catatctagc ctgcagcctg aggacttcgc cacctactac     720

tgccagcaga gctacagcac ccctttcaca tttggccctg gcaccaaggt ggacatcaaa     780

gagtctaagt acggaccgcc ctgcccccct tgccctatgt tctgggtgct ggtggtggtc     840

ggaggcgtgc tggcctgcta cagcctgctg gtcaccgtgg ccttcatcat cttttgggtg     900

aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa     960

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt    1020

gaactgcggg tgaagttcag cagaagcgcc gacgcccctg cctaccagca gggccagaat    1080

cagctgtaca acgagctgaa cctgggcaga agggaagagt acgacgtcct ggataagcgg    1140

agaggccggg accctgagat gggcggcaag cctcggcgga agaaccccca ggaaggcctg    1200

tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg catgaagggc    1260

gagcggaggc ggggcaaggg ccacgacggc ctgtatcagg gcctgtccac cgccaccaag    1320

gatacctacg acgccctgca catgcaggcc ctgcccccaa gg                       1362


<210> 31
<211> 473
<212> PRT
<213> Artificial Sequence


<220> 
<223> full-length CAR with IgG3 hinge and CD28 costimulatory domain

<400> 31
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
            260                 265                 270         
Cys Pro Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys 
        275                 280                 285             
Pro Arg Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
    290                 295                 300                 
Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
305                 310                 315                 320 
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
                325                 330                 335     
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            340                 345                 350         
Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        355                 360                 365             
Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    370                 375                 380                 
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
385                 390                 395                 400 
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                405                 410                 415     
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            420                 425                 430         
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        435                 440                 445             
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    450                 455                 460                 
Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470             

<210> 32
<211> 1419
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 31

<400> 32
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg      60

atccctaagg tgcagctgct ggaatctggc ggaggactgg ttcagcctgg cagaagcctg     120

agactgtctt gtgccgccag cggcttcacc ttcgacgatt atggcatgca ctgggtccga     180

caggcccctg gcaaaggact tgaatgggtg tccggcatca gctggaacag cggctctatc     240

ggctacgccg attccgtgaa gggcagattc accatcagcc gggacaacag caagaacacc     300

ctgtacctgc agatgaacag cctgagagcc gaggacaccg ccgtgtacta ttgtgctaga     360

ggcggccaga ccatcgacat ctggggacag ggaaccatgg tcaccgtttc tagcggaggc     420

ggaggttctg gcggcggagg aagtggtggc ggaggctctg atatccagat gacacagagc     480

cccagcagcc tgtctgcctc tgtgggagac agagtgacca tcacctgtag agccagccag     540

agcatcagca gctacctgaa ctggtatcag caaaagcccg gcaaggcccc taaactgctg     600

atctacgctg cctccagtct gcagagcgga gtgcctagca gattttctgg ctctggcagc     660

ggcaccgact tcaccctgac catatctagc ctgcagcctg aggacttcgc cacctactac     720

tgccagcaga gctacagcac ccctttcaca tttggccctg gcaccaaggt ggacatcaaa     780

gagctgaaaa cccctctggg cgacaccaca cacacatgcc ctagatgtcc ggaacccaag     840

agctgcgata cccccccacc ttgccccaga tgccccatgt tttgggtgct ggtggtcgtg     900

ggcggagtgc tggcctgtta cagcctgctc gtgaccgtgg ccttcatcat cttttgggtc     960

cgcagcaagc ggagcagagg cggccacagc gactacatga acatgacccc tagacggcct    1020

ggccccacca gaaagcacta ccagccctac gcccctcccc gggactttgc cgcctacaga    1080

agccgggtga agttcagcag aagcgccgac gcccctgcct accagcaggg ccagaatcag    1140

ctgtacaacg agctgaacct gggcagaagg gaagagtacg acgtcctgga taagcggaga    1200

ggccgggacc ctgagatggg cggcaagcct cggcggaaga acccccagga aggcctgtat    1260

aacgaactgc agaaagacaa gatggccgag gcctacagcg agatcggcat gaagggcgag    1320

cggaggcggg gcaagggcca cgacggcctg tatcagggcc tgtccaccgc caccaaggat    1380

acctacgacg ccctgcacat gcaggccctg cccccaagg                           1419


<210> 33
<211> 474
<212> PRT
<213> Artificial Sequence


<220> 
<223> full-length CAR with IgG3 hinge and 4-1BB costimulatory domain

<400> 33
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
            260                 265                 270         
Cys Pro Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys 
        275                 280                 285             
Pro Arg Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
    290                 295                 300                 
Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
305                 310                 315                 320 
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
                325                 330                 335     
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            340                 345                 350         
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        355                 360                 365             
Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    370                 375                 380                 
Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
385                 390                 395                 400 
Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                405                 410                 415     
Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            420                 425                 430         
Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        435                 440                 445             
Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    450                 455                 460                 
Ala Leu His Met Gln Ala Leu Pro Pro Arg 
465                 470                 

<210> 34
<211> 1422
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 33

<400> 34
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg      60

atccctaagg tgcagctgct ggaatctggc ggaggactgg ttcagcctgg cagaagcctg     120

agactgtctt gtgccgccag cggcttcacc ttcgacgatt atggcatgca ctgggtccga     180

caggcccctg gcaaaggact tgaatgggtg tccggcatca gctggaacag cggctctatc     240

ggctacgccg attccgtgaa gggcagattc accatcagcc gggacaacag caagaacacc     300

ctgtacctgc agatgaacag cctgagagcc gaggacaccg ccgtgtacta ttgtgctaga     360

ggcggccaga ccatcgacat ctggggacag ggaaccatgg tcaccgtttc tagcggaggc     420

ggaggttctg gcggcggagg aagtggtggc ggaggctctg atatccagat gacacagagc     480

cccagcagcc tgtctgcctc tgtgggagac agagtgacca tcacctgtag agccagccag     540

agcatcagca gctacctgaa ctggtatcag caaaagcccg gcaaggcccc taaactgctg     600

atctacgctg cctccagtct gcagagcgga gtgcctagca gattttctgg ctctggcagc     660

ggcaccgact tcaccctgac catatctagc ctgcagcctg aggacttcgc cacctactac     720

tgccagcaga gctacagcac ccctttcaca tttggccctg gcaccaaggt ggacatcaaa     780

gagctgaaaa cccctctggg cgacaccaca cacacatgcc ctagatgtcc ggaacccaag     840

agctgcgata cccccccacc ttgccccaga tgccccatgt tttgggtgct ggtggtcgtg     900

ggcggagtgc tggcctgtta cagcctgctc gtgaccgtgg ccttcatcat cttttgggtc     960

aagcggggca gaaagaagct gctgtacatc tttaagcagc ccttcatgcg gcccgtgcag    1020

accacccagg aagaggacgg ctgctcctgc agattccccg aggaagaaga aggcggctgc    1080

gagctgagag tgaagttcag cagatccgcc gacgcccctg cctatcagca gggccagaac    1140

cagctataca acgagctgaa cctgggcaga cgggaagagt acgacgtgct ggacaagaga    1200

agaggccggg accctgagat gggcggaaag cccagaagaa agaaccccca ggaaggcctg    1260

tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg aatgaagggc    1320

gagcggcgga gaggcaaggg ccacgatgga ctgtatcagg gcctgagcac cgccaccaag    1380

gacacctatg acgccctgca catgcaggcc ctgcccccta ga                       1422


<210> 35
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> extracellular domain with IgG4 hinge

<400> 35
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
            260                 265                 270         



<210> 36
<211> 816
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 35

<400> 36
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg      60

atccctaagg tgcagctgct ggaatctggc ggaggactgg ttcagcctgg cagaagcctg     120

agactgtctt gtgccgccag cggcttcacc ttcgacgatt atggcatgca ctgggtccga     180

caggcccctg gcaaaggact tgaatgggtg tccggcatca gctggaacag cggctctatc     240

ggctacgccg attccgtgaa gggcagattc accatcagcc gggacaacag caagaacacc     300

ctgtacctgc agatgaacag cctgagagcc gaggacaccg ccgtgtacta ttgtgctaga     360

ggcggccaga ccatcgacat ctggggacag ggaaccatgg tcaccgtttc tagcggaggc     420

ggaggttctg gcggcggagg aagtggtggc ggaggctctg atatccagat gacacagagc     480

cccagcagcc tgtctgcctc tgtgggagac agagtgacca tcacctgtag agccagccag     540

agcatcagca gctacctgaa ctggtatcag caaaagcccg gcaaggcccc taaactgctg     600

atctacgctg cctccagtct gcagagcgga gtgcctagca gattttctgg ctctggcagc     660

ggcaccgact tcaccctgac catatctagc ctgcagcctg aggacttcgc cacctactac     720

tgccagcaga gctacagcac ccctttcaca tttggccctg gcaccaaggt ggacatcaaa     780

gagtctaagt acggaccgcc ctgcccccct tgccct                               816


<210> 37
<211> 292
<212> PRT
<213> Artificial Sequence


<220> 
<223> extracellular domain with IgG3 hinge

<400> 37
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro Lys Val Gln Leu Leu Glu Ser Gly Gly Gly 
            20                  25                  30          
Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              
Phe Thr Phe Asp Asp Tyr Gly Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  
Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  
Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         
Thr Ala Val Tyr Tyr Cys Ala Arg Gly Gly Gln Thr Ile Asp Ile Trp 
        115                 120                 125             
Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser 
145                 150                 155                 160 
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys 
                165                 170                 175     
Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn Trp Tyr Gln Gln Lys 
            180                 185                 190         
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln 
        195                 200                 205             
Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
    210                 215                 220                 
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
225                 230                 235                 240 
Cys Gln Gln Ser Tyr Ser Thr Pro Phe Thr Phe Gly Pro Gly Thr Lys 
                245                 250                 255     
Val Asp Ile Lys Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr 
            260                 265                 270         
Cys Pro Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys 
        275                 280                 285             
Pro Arg Cys Pro 
    290         

<210> 38
<211> 876
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 37

<400> 38
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg      60

atccctaagg tgcagctgct ggaatctggc ggaggactgg ttcagcctgg cagaagcctg     120

agactgtctt gtgccgccag cggcttcacc ttcgacgatt atggcatgca ctgggtccga     180

caggcccctg gcaaaggact tgaatgggtg tccggcatca gctggaacag cggctctatc     240

ggctacgccg attccgtgaa gggcagattc accatcagcc gggacaacag caagaacacc     300

ctgtacctgc agatgaacag cctgagagcc gaggacaccg ccgtgtacta ttgtgctaga     360

ggcggccaga ccatcgacat ctggggacag ggaaccatgg tcaccgtttc tagcggaggc     420

ggaggttctg gcggcggagg aagtggtggc ggaggctctg atatccagat gacacagagc     480

cccagcagcc tgtctgcctc tgtgggagac agagtgacca tcacctgtag agccagccag     540

agcatcagca gctacctgaa ctggtatcag caaaagcccg gcaaggcccc taaactgctg     600

atctacgctg cctccagtct gcagagcgga gtgcctagca gattttctgg ctctggcagc     660

ggcaccgact tcaccctgac catatctagc ctgcagcctg aggacttcgc cacctactac     720

tgccagcaga gctacagcac ccctttcaca tttggccctg gcaccaaggt ggacatcaaa     780

gagctgaaaa cccctctggg cgacaccaca cacacatgcc ctagatgtcc ggaacccaag     840

agctgcgata cccccccacc ttgccccaga tgcccc                               876


<210> 39
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> intracellular domain with CD28 costimulatory domain

<400> 39
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              
Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            100                 105                 110         
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    130                 135                 140                 
Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             

<210> 40
<211> 459
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 39

<400> 40
cgcagcaagc ggagcagagg cggccacagc gactacatga acatgacccc tagacggcct      60

ggccccacca gaaagcacta ccagccctac gcccctcccc gggactttgc cgcctacaga     120

agccgggtga agttcagcag aagcgccgac gcccctgcct accagcaggg ccagaatcag     180

ctgtacaacg agctgaacct gggcagaagg gaagagtacg acgtcctgga taagcggaga     240

ggccgggacc ctgagatggg cggcaagcct cggcggaaga acccccagga aggcctgtat     300

aacgaactgc agaaagacaa gatggccgag gcctacagcg agatcggcat gaagggcgag     360

cggaggcggg gcaagggcca cgacggcctg tatcagggcc tgtccaccgc caccaaggat     420

acctacgacg ccctgcacat gcaggccctg cccccaagg                            459


<210> 41
<211> 154
<212> PRT
<213> Artificial Sequence


<220> 
<223> intracellular domain with 4-1BB costimulatory domain

<400> 41
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              
Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  
Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  
Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      
Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         
Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             
Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 
Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 

<210> 42
<211> 462
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding SEQ ID NO: 41

<400> 42
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa      60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt     120

gaactgcggg tgaagttcag cagaagcgcc gacgcccctg cctaccagca gggccagaat     180

cagctgtaca acgagctgaa cctgggcaga agggaagagt acgacgtcct ggataagcgg     240

agaggccggg accctgagat gggcggcaag cctcggcgga agaaccccca ggaaggcctg     300

tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg catgaagggc     360

gagcggaggc ggggcaaggg ccacgacggc ctgtatcagg gcctgtccac cgccaccaag     420

gatacctacg acgccctgca catgcaggcc ctgcccccaa gg                        462


<210> 43
<211> 225
<212> DNA
<213> Artificial Sequence


<220> 
<223> left IR/DR segment

<400> 43
cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac     60

tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact    120

ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata    180

attcactgta tcacaattcc agtgggtcag aagtttacat acact                    225


<210> 44
<211> 225
<212> DNA
<213> Artificial Sequence


<220> 
<223> right IR/DR segment

<400> 44
agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa     60

tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac    120

tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt    180

taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactg                    225


<210> 45
<211> 340
<212> PRT
<213> Artificial Sequence


<220> 
<223> Sleeping Beauty amino acid sequence

<400> 45
Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      
Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          
Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              
His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  
Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  
Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      
Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         
Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             
Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 
Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 
Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     
Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         
Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             
Ile Asp Gly Ile Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 
His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 
Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     
Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         
Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             
Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 
Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 
Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     
Ala Thr Lys Tyr 
            340 

<210> 46
<211> 1359
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence encoding full-length siglec-6

<400> 46
atgcagggag cccaggaagc ctccgcctca gagatgctac cgctgctgct gcccctgctg      60

tgggcagggg ccctggctca ggagcggaga ttccagctgg aggggccaga gtcactgacg     120

gtgcaggagg gtctgtgcgt cctcgtaccc tgcagattgc ccactaccct tccagcctcg     180

tactatggtt atggctactg gttcctggaa ggggctgatg ttccagtggc cacaaacgac     240

ccagacgaag aagtgcagga ggagacccgg ggccgattcc acctcctctg ggatcccaga     300

aggaagaact gctccctgag catcagagat gcccggagga gggacaatgc tgcatacttc     360

tttcggttga agtccaaatg gatgaaatac ggttatacat cttccaagct ctctgtgcgt     420

gtgatggccc tgacccacag gcccaacatc tccatcccag ggaccctgga gtctggccat     480

cccagcaatc tgacctgctc tgtgccctgg gtctgtgagc aggggacgcc ccccatcttc     540

tcctggatgt cagctgcccc cacctccctg ggccccagga ccacccagtc ctcggtgctc     600

acaatcaccc cacggcccca ggaccacagc accaacctca cctgtcaggt gacgttccct     660

ggagccggtg tgaccatgga gagaaccatc cagctcaatg tctcctatgc tccacagaaa     720

gtggccatca gcatcttcca aggaaacagc gcagccttca aaatcctgca aaacacctcg     780

tccctccctg tcctggaggg ccaggctctg cggctgctct gtgatgctga cggcaacccc     840

cctgcacacc tgagctggtt ccagggcttc cccgccctga acgccacccc catctccaat     900

accggggtcc tggagctgcc tcaagtaggg tctgcagaag aaggagattt cacctgccgt     960

gctcagcatc ctctgggctc cctgcaaatc tctctgagtc tctttgtgca ttggaaacca    1020

gaaggcaggg ctggtggtgt cctgggagca gtctggggag ctagcatcac aaccctggtt    1080

ttcctctgtg tttgcttcat cttcagagtg aagactagaa ggaagaaagc agcccagcca    1140

gtgcaaaaca cggatgatgt gaaccccgtc atggtctcag gctccagggg tcatcagcac    1200

cagttccaga caggcatagt ttcagaccac cctgctgagg ctggccccat ctcagaagat    1260

gagcaggagc tccactacgc tgtcctacac ttccacaagg tgcaacctca ggaaccaaag    1320

gtcaccgaca ctgagtactc agaaatcaag atacacaag                           1359


